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Implant infection is one of the most common and serious complications in orthopedics, and it is also an important reason for the failure of implant surgery. When bacteria form a biofilm on the implant surface, it is extremely difficult to be eliminated and attracts more bacteria and fungi. A large number of studies have shown that the use of surface modification technology can effectively reduce the adhesion and accumulation of pathogenic bacteria, thereby preventing peri-implant infection. The formation process of bacterial biofilm on the surface of orthopedic implants and the antibacterial mechanism of metal antibacterial agents were first analyzed. Then, some of the most widely used metal-based inorganic antibacterial coatings at home and abroad and their related preparation processes were reviewed, the problems and improvement methods in the application of these coatings were also discussed, and the development direction of inorganic antibacterial coatings in the future was prospected, such as synergistic antibacterial coatings and bone-promoting antibacterial coatings.

Safety production is an important way to ensure the personal safety of workers and the safety of national property. This project focuses on addressing the safety generation problem in the power marketing profession, designing a set of intelligent dynamic warning systems for power marketing operations. MobileNetV1 is used as the backbone feature extraction network of SSD to create a lightweight target detection network, and it is combined with OpenVINO and NCS2 neural computation bars to construct an image analysis and dynamic warning model for power operation based on the SSD MobileNetV1 algorithm. We use the images of power marketing operation sites in a city as a dataset to test and compare the constructed model with other target detection algorithms, analyzing its dynamic warning effect on power operations and exploring the effectiveness of the model’s practical application. The results show that the SSD network model can significantly improve the detection efficiency and accuracy of the dynamic early warning system for electric power operations, and its accuracy is 15.15% and 18.82% higher than that of the Faster⁃RCNN algorithm and the YOLO algorithm, respectively. The early warning system reduced the irregular behavior of electric power marketing operations by 135.71%, demonstrating the effectiveness of the SSD model in detecting and warning electric power targets. This model not only meets practical requirements but also effectively reduces the irregular production behaviors of electric power marketing operations, thereby enhancing the safety coefficient of marketing field operations.

C19H16Br4CuN2O2: monoclinic, P21/c (no. 14), a = 10.2649(3) Å, b = 10.1903(3) Å, c = 21.2494(6) Å, β = 100.522(1)°, V = 2185.36(11) Å3, Z = 4, wR 2 = 0.0730, T = 169.0 K.

C22H26Br2MnN5O4, monoclinic, P21/n (no. 14), a = 8.0106(4) Å, b = 14.5925(7) Å, c = 21.0425(11) Å, β = 100.330(2)°, V = 2419.9(2) Å3, Z = 4, Rgt(F) = 0.0372, wRref(F2) = 0.0766, T = 296.15 K.

C19H18Cl2CuN2O2, monoclinic, space group C2/c (no. 15), a = 21.010(3) Å, b = 11.3886(15) Å, c = 7.9578(10) Å, β = 100.725(4)°, V = 1870.8(4) Å3, Z = 4, wR 2 = 0.1620, T = 170.0 K.

C14H10BrClN2O2, orthorhombic, Pca21 (no. 29), a = 32.4137(12) Å, b = 4.6683(2) Å, c = 8.8873(3) Å, V = 1344.80(9) Å3, Z = 4, Rgt(F) = 0.0239, wRref(F2) = 0.0611, T = 150(2) K.

C15H14Br2N2O3, triclinic, P1‾$P‾{1}$ (no. 2), a = 7.2748(3) Å, b = 9.0786(4) Å, c = 12.8613(5) Å, α = 99.458(2)°, β = 100.401(2)°, γ = 99.499(2)°, V = 807.35(6) Å3, Z = 2, Rgt(F) = 0.0253, wRref(F2) = 0.0673, T = 150.0 K.

C20H18Br2Cl2N2O2, monoclinic, P21/c (no. 14), a = 10.6105(8) Å, b = 17.1664(9) Å, c = 11.9848(9) Å, β = 97.682(3)°, V = 2163.4(3) Å3, Z = 4, Rgt(F) = 0.0330, wRref(F2) = 0.0823, T = 100(2) K.

Blastocystis sp., a parasitic eukaryote, widely colonizes the intestines of humans and a large number of animals, including rodents and lagomorphs. More than 30 million bamboo rats (Rhizomys sinensis) are farmed in China as a source of meat for human consumption. However, there have been no published articles on Blastocystis infection in Chinese bamboo rats prior to the present study. Herein, 480 fresh faecal samples were collected from R. sinensis on six farms located in four cities (Wugang, Chenzhou, Huaihua and Jishou) in Hunan Province, south-central China, and were examined for Blastocystis infection using polymerase chain reaction (PCR) targeting the small subunit ribosomal RNA (SSU rRNA) gene. The total prevalence of Blastocystis in R. sinensis was 4.58% (22/480), and significant differences in prevalence were detected among four age groups (24 months), with the highest prevalence (7.81%) in rats aged 6–12 months but with no positive samples in rats over 24 months. All farms, except for one in Jishou, were positive for Blastocystis infection, with the prevalence ranging from 1.80% to 7.27%. Sequence and phylogenetic analyses revealed two potentially zoonotic subtypes (namely ST4 and ST5) in these rodents, with ST4 predominant in all except one farm in Huaihua. Seven and five sequence types were identified within ST4 and ST5, respectively. This is the first report of Blastocystis infection in Chinese bamboo rats and the findings suggest the potential of R. sinensis to transmit Blastocystis to humans.

The aim of this study was to investigate the effect of PV modules mounted on top of a greenhouse, on the growth of strawberries and microclimate conditions as well as to estimate the generated energy. In this study, two greenhouses with the same volume were established. One greenhouse was equipped with the opaque photovoltaic (OPV) modules which accounted for 25.9% of the roof area, and the other was equipped with the semi-transparent photovoltaic (STPV) modules which accounted for 20% of the roof area. The maximum annual power generation of OPV and STPV modules was 880 and 388 kWh with 30° tilt angle, respectively, by simulating different tilt angles. The temperature under the OPV and STPV modules was 2.9 and 1.1 °C lower than the unshaded part in the greenhouses, respectively, at noon in clear weather, and had little effect on relative humidity. The photosynthetically active radiation (PAR) under OPV and STPV modules was reduced by 43.5% and 31.7%, respectively, under the PE film greenhouse. The contents of soluble solids in strawberries in OPV and STPV greenhouses were 16.4 and 15.7 mg/g respectively, which were higher than those in unshaded samples. The quality and yield of the strawberry samples under the shade of OPV were better than those of the STPV shade.

BackgroundThe monoclonal antibody daratumumab, approved for treating myeloma, targets CD38, a protein on myeloma and also on CD34+ hematopoietic progenitor cells. Because mobilized CD34+ cells are critical for stem cell transplant, we investigated the in vitro activity of daratumumab on mobilized CD34+ cells from myeloma patients with no prior exposure to daratumumab.MethodsWe determined the number of CD38 molecules per CD34+ cell, and whether daratumumab bound to CD34+ cells, whether C1q bound to daratumumab-coated CD34+ cells and whether daratumumab-related complement-dependent cytotoxicity (CDC) occurred. We also examined CD34+ cell progenitor cell colony capacity in assays with pre-plating incubation of CD34+ cells with daratumumab alone or with daratumumab and the CD59 inhibitory antibody BRIC229, and also assessed CD34+ cell responses to increasing doses of daratumumab in caspase 3/7 activity assays.ResultsAlthough 75% of mobilized CD34+ cells co-express CD38, CD38 was minimally present on CD34+ cells compared to Daudi and KG-1 controls, C1q did not bind to daratumumab-coated CD34+ cells, and CDC did not occur. CD34+ cells incubated in complement-rich human serum with daratumumab alone or with daratumumab and BRIC229, and then plated in progenitor cell assays, produced similar numbers of colonies as controls. In progenitor cell assays with cryopreserved or fresh unselected or CD34-selected cells, daratumumab did not affect progenitor cell capacity, and in caspase 3/7 activity assays CD34+ cells were not affected by increasing doses of daratumumab.ConclusionIn vitro, daratumumab is not toxic to mobilized CD34+ progenitor cells from myeloma patients.

Simple Summary: Listeria pathogenicity island 4 (LIPI-4)—which is composed of six genes, including EⅡB—has been identified as a specific virulence factor closely related to infections of the nervous system and the placenta. This study examined the virulence effect of LIPI-4 EⅡB. Deletion of EⅡB reduced the biofilm formation ability and cell intracellular proliferation capacity of Listeria monocytogenes, as well as bacterial load in mice. In addition, EⅡB was found to regulate the transcription levels of genes related to virulence and biofilm formation. These findings provide a foundation for further examinations of the pathogenic mechanisms of LIPI-4 and EⅡB in L. monocytogenes. To explore the role of the membrane permease ⅡB (EⅡB) gene of Listeria pathogenicity island 4 (LIPI-4) in the virulence of Listeria monocytogenes, both an EⅡB deletion strain (∆EⅡB) and a complemented strain were constructed. In vitro experiments demonstrated that EⅡB deletion affected the biofilm formation ability of the wild-type strain (Lm928). Moreover, this deletion decreased the intracellular proliferation abilities of L. monocytogenes. Mice infected with ∆EⅡB survived longer and experienced less weight loss on days 1, 2, and 3 post-infection. The bacterial load in the liver tissue of ∆EⅡB-infected mice was significantly reduced, and a considerable decrease in the blood levels of inflammatory cytokines IL-β, IL-6, IL-10, and TNF-α were observed. Following EⅡB deletion, 65% (13/20) of genes were downregulated, 25% (5/20) were upregulated, and 10% (2/20) showed no change. These findings suggest that EⅡB deletion may reduce both the in vivo and in vitro virulence levels as well as the biofilm formation ability of Lm928 by downregulating the transcription levels of genes associated with virulence and biofilm formation. These findings provide a foundation for further examining the pathogenic mechanisms of LIPI-4 and EⅡB in L. monocytogenes. [ABSTRACT FROM AUTHOR]

Listeria monocytogenes (L. monocytogenes) is a foodborne pathogen that causes listeriosis in humans and other animals. Surface proteins with the LPXTG motif have important roles in the virulence of L. monocytogenes. Lmo0159 is one such protein, but little is known about its role in L. monocytogenes virulence, motility, and biofilm formation. Here, we constructed and characterized a deletion mutant of lmo0159 (∆lmo0159). We analyzed not only the capacity of biofilm formation, motility, attachment, and intracellular growth in different cell types but also LD50; bacterial load in mice's liver, spleen, and brain; expression of virulence genes; and survival time of mice after challenge. The results showed that the cross-linking density of the biofilm of ∆lmo0159 strain was lower than that of WT by microscopic examination. The expression of biofilm-formation and virulence genes also decreased in the biofilm state. Subsequently, the growth and motility of ∆lmo0159 in the culture medium were enhanced. Conversely, the growth and motility of L. monocytogenes were attenuated by ∆lmo0159 at both the cellular and mouse levels. At the cellular level, ∆lmo0159 reduced plaque size; accelerated scratch healing; and attenuated the efficiency of adhesion, invasion, and intracellular proliferation in swine intestinal epithelial cells (SIEC), RAW264.7, mouse-brain microvascular endothelial cells (mBMEC), and human-brain microvascular endothelial cells (hCMEC/D3). The expression of virulence genes was also inhibited. At the mouse level, the LD50 of the ∆lmo0159 strain was 100.97 times higher than that of the WT strain. The bacterial load of the ∆lmo0159 strain in the liver and spleen was lower than that of the WT strain. In a mouse model of intraperitoneal infection, the deletion of the lmo0159 gene significantly prolonged the survival time of the mice, suggesting that the lmo0159 deletion mutant also exhibited reduced virulence. Thus, our study identified lmo0159 as a novel virulence factor among L. monocytogenes LPXTG proteins. [ABSTRACT FROM AUTHOR]