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Objective This study investigated the efficacy of different concentrations of cholesterol-loaded cyclodextrin (CLC) on cryopreservation in boar sperm quality. Methods In this study, we treated boar sperm with different concentrations of CLC before freezing and analyzed the sperm cholesterol concentration, plasma membrane, acrosome integrity rate and total motility rate before and after freeze-thawing. We also investigated the levels of reactive oxygen species (ROS), malondialdehyde (MDA), adenosine triphosphate (ATP), and structural- and oxidative-damage related proteins in all groups after thawing. Results The results revealed that the cholesterol concentration of the CLC-treated groups was higher than that of the control group, both before freezing and after thawing (p

Antimicrobial peptide (AMP) surfaces are widely used to inhibit biofilm formation and bacterial infection. However, endpoint-immobilized AMPs on surfaces are totally different from free-state AMPs due to the constraints of the surface. In this work, the interactions between AMPs and bacterial cell membranes were analyzed through coarse-grained molecular dynamics and all-atom molecular dynamics simulations. This AMP disrupted membrane structure by altering the thickness and curvature of the membrane. Furthermore, the effect of surface-immobilized states of AMPs on their ability to disrupt membrane structure was revealed. The immobilized AMPs in the freeze-N system could bind to the membrane and disrupt the membrane structure through electrostatic forces between positively charged N-terminal amino acid residues and the negatively charged membrane, while the immobilized AMPs in the freeze-C system were repelled. The results will aid in the rational design of new AMP surfaces with enhanced efficacy and stability. [ABSTRACT FROM AUTHOR]

The fact that tension fabric structures are light and durable, as well as visually pleasing, has become a reason for preference, especially in contemporary architecture. In this article, in addition to a comprehensive evaluation of stretchable fabric structures, two important stretchable fabric systems, Bigo Arena and Denver International Airport, are used as case studies. By comparing these two case studies, this study pays attention to the differences and properties of the materials while also emphasizing the advantages of the tensile fabric structure. It aims to give ideas to researchers working on this subject by researching the materials and techniques used during construction and examining the possibilities, such as their impact on progress and sustainability in architecture. The Denver International Airport and Bigo Arena serve as prime examples of how membrane systems may be used successfully in architectural projects. While PTFE-coated glass fiber material is preferred in the construction of lightweight structures, it also provides the opportunity to capture solar radiation. Through the projects examined in this research, it is emphasized that fiberglass material coated with PTFE is an example of how interior spaces can be more useful by creating both aesthetic and well-lit spaces. In summary, the case studies demonstrate the successful application of membrane frameworks to achieve functional and aesthetically acceptable goals in architecture.

Nonsolvent-induced phase separation is a widely used technique in the manufacture of polymeric separators. This method involves fine-tuning porous structures through the phase separation of polymer solutions in Li-ion secondary battery systems. The phase separation properties and kinetics of heat-resistant poly(vinylidene fluoride-co-hexafluoropropylene) polymer solutions were characterized by adjusting the weight ratio of N-methyl-2-pyrrolidone (NMP) as a solvent and water as a nonsolvent using both macro- and micro-rheological techniques. The viscoelastic moduli, measured with a rotational rheometer as a macro-rheological technique, and autocorrelation functions describing the fast movements of tracer ceria particles within polymer solutions—quickly detected using the micro-rheological light scattering technique of multi-speckle diffusing wave spectroscopy—offered a comprehensive assessment of the phase separation status and its kinetics during changes in the NMP/water ratio. These results are expected to play a fundamental role in understanding and controlling the pore structures of actual separator membranes applied in Li-ion battery systems. [ABSTRACT FROM AUTHOR]

We describe how to use several machine learning techniques organized in a learning pipeline to segment and identify cell membrane structures from cryo electron tomograms. These tomograms are difficult to analyze with traditional segmentation tools. The learning pipeline in our approach starts from supervised learning via a special convolutional neural network trained with simulated data. It continues with semi-supervised reinforcement learning and/or a region merging technique that tries to piece together disconnected components belonging to the same membrane structure. A parametric or non-parametric fitting procedure is then used to enhance the segmentation results and quantify uncertainties in the fitting. Domain knowledge is used in generating the training data for the neural network and in guiding the fitting procedure through the use of appropriately chosen priors and constraints. We demonstrate that the approach proposed here works well for extracting membrane surfaces in two real tomogram datasets.

Although various studies on vibration control schemes exist, most of them focus on a one-time optimization of actuator layout. However, numerical results indicate significant drawbacks of fixed layouts on control performance, a factor rarely mentioned in the field of vibration control. This study aims to address this gap by proposing a novel rearrangement strategy that adaptively varies the actuator layout based on the vibration intensity pattern. The inspiration for this approach comes from the reorientation of cortical microtubules in plant cells, which adapt to stress patterns. The study first derives the definitions of vibration patterns and the index of intensity based on energy transfer. These definitions then guide the optimization of actuator layout throughout the control process. The study compares the fixed layout with the proposed adaptive varying layout, considering both full-state feedback and recognition feedback. The numerical results demonstrate that the proposed adaptive rearrangement of actuator layout significantly improves suppression performance, leading to higher utilization efficiency of control forces. Additionally, the presented strategy is tolerant to computation errors from recognition feedback, resulting in robust enhancements in vibration attenuation. Our study has introduced new possibilities for improving existing control schemes. • First-time practical dynamic rearrangement strategy adapts actuator layout based on system responses. • A bioinspired optimization framework aligns the actuator layout with a vibration intensity pattern in a fast-computing manner. • The strategy is compatible with both full-state feedback and recognition reconstructed from sensing signals. • Simulations show improved control performance with increased force utilization and error tolerance. [ABSTRACT FROM AUTHOR]

The organelles of eukaryotic cells maintain distinct protein and lipid compositions required for their specific functions. The mechanisms by which many of these components are sorted to their specific locations remain unknown. While some motifs mediating subcellular protein localization have been identified, many membrane proteins and most membrane lipids lack known sorting determinants. A putative mechanism for sorting of membrane components is based on membrane domains known as lipid rafts, which are laterally segregated nanoscopic assemblies of specific lipids and proteins. To assess the role of such domains in the secretory pathway, we applied a robust tool for synchronized secretory protein traffic (RUSH, Retention Using Selective Hooks) to protein constructs with defined affinity for raft phases. These constructs consist solely of single-pass transmembrane domains (TMDs) and, lacking other sorting determinants, constitute probes for membrane domain-mediated trafficking. We find that while raft affinity can be sufficient for steady-state PM localization, it is not sufficient for rapid exit from the endoplasmic reticulum (ER), which is instead mediated by a short cytosolic peptide motif. In contrast, we find that Golgi exit kinetics are highly dependent on raft affinity, with raft preferring probes exiting the Golgi ~2.5-fold faster than probes with minimal raft affinity. We rationalize these observations with a kinetic model of secretory trafficking, wherein Golgi export can be facilitated by protein association with raft domains. These observations support a role for raft-like membrane domains in the secretory pathway and establish an experimental paradigm for dissecting its underlying machinery.

Research on porous membrane technology is proliferating, especially in the process of fabrication of membranes. Different methods in membrane fabrication can affect the physical and chemical properties of the produced membrane. This study aims to investigate the influence of temperature, molecular weight, and non-solvent type on the physical-chemical properties of PVDF membranes. The membrane was produced by the immersion precipitation method with varying PVDF molecular weights of 64 kDa, 352 kDa (Solef 1010), 534 kDa, and 573 kDa (Solef 1015); non-solvent variations of alcohol (methanol, ethanol, isopropyl alcohol, and butanol); and drying temperature variations of 40, 50, and 60°C. The produced membranes were analyzed using ATR-FTIR, XRD, TGA, DSC, and SEM, and their wettability properties were evaluated using water contact angles. The optimal drying temperature for membrane production was 60°C. The ATR-FTIR data showed that molecular weight impacted membrane structure, where PVDF MW 534 kDa membrane had the highest percentage of β phase (77.47%). Non-solvent changes also affected membrane structure; PVDF Solef 1010 with non-solvent isopropyl alcohol had the highest percentage of β phase (67.45%). This is supported by the XRD diffractogram that displayed peaks at 2θ values between 20.24° and 20.66°, indicating the presence of a phase β PVDF. The thermal analysis exhibited three stages of degradation for Solef 1010 with ethanol non-solvent and two for the other seven membranes. The degradation temperature increases with the increase in molecular weight and the difference in non-solvents. The highest thermal stability membrane was PVDF Solef 1010 with isopropyl alcohol non-solvent (430°C). SEM images showed the membrane with non-solvent isopropyl alcohol, displaying a dense sponge-like morphology. The wettability of membranes is affected by molecular weight and non-solvent type. The membrane with isopropyl alcohol non-solvent obtained the smallest contact angle (54.77°) and indicated the most wettability membrane.

Antimicrobial peptide (AMP) surfaces are widely used to inhibit biofilm formation and bacterial infection. However, endpoint-immobilized AMPs on surfaces are totally different from free-state AMPs due to the constraints of the surface. In this work, the interactions between AMPs and bacterial cell membranes were analyzed through coarse-grained molecular dynamics and all-atom molecular dynamics simulations. This AMP disrupted membrane structure by altering the thickness and curvature of the membrane. Furthermore, the effect of surface-immobilized states of AMPs on their ability to disrupt membrane structure was revealed. The immobilized AMPs in the freeze-N system could bind to the membrane and disrupt the membrane structure through electrostatic forces between positively charged N-terminal amino acid residues and the negatively charged membrane, while the immobilized AMPs in the freeze-C system were repelled. The results will aid in the rational design of new AMP surfaces with enhanced efficacy and stability.

Rice sheath blight is a fungal disease caused mainly by Rhizoctonia solani AG1-IA. Toxins are a major pathogenic factor of R. solani, and some studies have reported their toxin components; however, there is no unified conclusion. In this study, we reported the toxin components and their targets that play a role in R. solani AG1-IA. First, toxins produced by R. solani AG1-IA were examined. Several important phytotoxins, including benzoic acid (BZA), 5-hydroxymethyl-2-furanic aid (HFA), and catechol (CAT), were identified by comparative analysis of secondary metabolites from AG1-IA, AG1-IB, and healthy rice. Follow-up studies have shown that the toxin components of this fungus can rapidly disintegrate the biofilm structure while maintaining the content of host plant membrane components, thereby affecting the organelles, which may also explain the lack of varieties highly resistant to sheath blight. [ABSTRACT FROM AUTHOR]

Research on porous membrane technology is proliferating, especially in the process of fabrication of membranes. Different methods in membrane fabrication can affect the physical and chemical properties of the produced membrane. This study aims to investigate the influence of temperature, molecular weight, and non-solvent type on the physical-chemical properties of PVDF membranes. The membrane was produced by the immersion precipitation method with varying PVDF molecular weights of 64 kDa, 352 kDa (Solef 1010), 534 kDa, and 573 kDa (Solef 1015); non-solvent variations of alcohol (methanol, ethanol, isopropyl alcohol, and butanol); and drying temperature variations of 40, 50, and 60°C. The produced membranes were analyzed using ATR-FTIR, XRD, TGA, DSC, and SEM, and their wettability properties were evaluated using water contact angles. The optimal drying temperature for membrane production was 60°C. The ATR-FTIR data showed that molecular weight impacted membrane structure, where PVDF MW 534 kDa membrane had the highest percentage of ß phase (77.47%). Nonsolvent changes also affected membrane structure; PVDF Solef 1010 with nonsolvent isopropyl alcohol had the highest percentage of β phase (67.45%). This is supported by the XRD diffractogram that displayed peaks at 2° values between 20.24° and 20.66°, indicating the presence of a phase β PVDF. The thermal analysis exhibited three stages of degradation for Solef 1010 with ethanol nonsolvent and two for the other seven membranes. The degradation temperature increases with the increase in molecular weight and the difference in nonsolvents. The highest thermal stability membrane was PVDF Solef 1010 with isopropyl alcohol non-solvent (430°C). SEM images showed the membrane with non-solvent isopropyl alcohol, displaying a dense sponge-like morphology. The wettability of membranes is affected by molecular weight and non-solvent type. The membrane with isopropyl alcohol non-solvent obtained the smallest contact angle (54.77°) and indicated the most wettability membrane. [ABSTRACT FROM AUTHOR]

The dynamic of hypervelocity projectile impacting inflatable multilayer flexible membrane structure is studied in this paper. The velocity increment of the inflatable multilayer flexible membrane structure under hypervelocity impact comes from two parts. One is the acceleration caused by the pulling of the membrane when the hypervelocity projectile is in direct contact with the membrane, and the other is the acceleration provided by the reverse impulse caused by the gas leakage in the inflatable structure. The hypervelocity projectile will be decelerated in the impact process, and finally fly with the accelerated multilayer flexible membrane structure at the same velocity, which means that the projectile is captured by the membrane structure. According to the above process, the influence of the mechanical property parameters of the flexible membrane on the structural parameters of the multi-layer flexible membrane can be calculated, which can be used to guide the engineering design. Finally, the hypervelocity impact process is verified by the numerical simulation. • Space soft capture method achieved through flexible inflatable membrane structure. • Hypervelocity impact dynamics of flexible inflatable membrane structure. • Verify the dynamic process of hypervelocity impact using numerical simulation. • The influence of membrane mechanical properties on the parameters of the structure. [ABSTRACT FROM AUTHOR]

The existing large inflatable membrane structure is complex in structure and takes a long time to be extended. In this paper, an air-ribbed skeleton membrane structure is proposed to meet the requirement of camp and emergency tents needing to be set up quickly and easily. Ten single-arch air ribs are arranged side by side to support the tarpaulin, and wind ropes are added to the end and side faces of the tarpaulin to remain stable. The finite element model of the air-ribbed skeleton membrane structure is established to analyze the stress and the displacement of the membrane structure under combined wind and snow loads. The maximum displacement (439.4 mm) and maximum stress (29.94 MPa) are both within the safe standard. The stress and the displacement of the membrane structure in the wind load case is affected by the angle of the wind. The value of maximum stress and displacement at the wind angle of 90° are both lower than those at 0° and 45° in the wind load case. It is advisable to align the site layout of the membrane structure at the wind angle of 90°. The effect of the angle of wind ropes on the stress and displacement of the membrane structure is also studied. The maximum stress and displacement in the case when the angle of wind rope is 30° is smaller than those in the case when the angle of wind ropes is 45°. It is recommended that the wind rope should be laid at 30° to reinforce the membrane structure. [ABSTRACT FROM AUTHOR]

Lipids constitute a significant group of biological metabolites and the building blocks of all cell membranes. The abundance and stoichiometries of different lipid species are known to vary across the lifespan and metabolic state, yet the functional effects of these changes have been challenging to understand. Here we review the potentially powerful intersection of lipid metabolism, which determines membrane composition, and aging. We first introduce several key lipid classes that are associated with aging and aging-related disease, where they are found in organisms, and how they act on membrane structure and function. Instead of neutral lipids, which have primary roles in energy storage and homeostasis, we review known functions for polar lipids that control the physicochemical properties of cell membranes. We then focus on aging processes in the central nervous system (CNS), which is enriched in lipids and is highly dependent on membrane structure for function. Recent studies show how lipids act not just as biomarkers of aging and associated changes in the CNS, but as direct mediators of these processes. As a model system, we explore how fatty acid composition in the retina impact aging and aging-related disease. We propose that the biophysical effects of membrane structure on fundamental eukaryotic processes - mitochondrial respiration and autophagy - provide avenues by which lipid dysregulation can accelerate aging processes. Finally, we lay out ways in which an increased understanding of lipid membrane biology can be applied to studies of aging and lifespan.

Introduction: Antifreeze peptides regulate the physiological functions of frozen cells and even their apoptosis; however, the mechanisms by which antifreeze peptides regulate these processes remain unclear, although the interactions between cell membranes and ice are well known to be important in this process. Objectives: Our study aims to investigate how antifreeze peptides regulate cell physiological functions during the freezing process. Methods: We investigated the cryoprotective effect of rsfAFP on the physiological functions of S. thermophilus under freezing stress by measuring cellular metabolism activity, intracellular enzyme activity, cell membrane characterization, and cell apoptosis. The mechanism by which rsfAFP impacts S. thermophilus physiological functions under freezing stress was investigated using multispectral techniques and cryo-TEM. Results: We show that a recombinant antifreeze peptide (rsfAFP) interacts with the extracellular capsular polysaccharides and peptidoglycan of Streptococcus thermophilus and ice to cover the outer layer of the membrane, forming a dense protective layer that regulates the molecular structure of extracellular ice crystals, which results in reduced extracellular membrane damage, depressed apoptosis and increased intracellular metabolic activity. This interaction mechanism was indicated by the fact that S. thermophilus better maintained its permeability barrier, membrane fluidity, membrane structural integrity, and cytoplasmic membrane potential during freezing stress with rsfAFP treatment. Conclusion: These results provide new insights into the mechanism by which rsfAFP regulates frozen cell physiological functions and apoptosis under freezing stress.

The membrane structure roof is widely used in large-span buildings such as stadiums and gymnasiums because of its full use of natural light sources and flexible forms. In order to solve the prominent problems such as poor thermal insulation performance and prone to external environmental factors, the multi-layer membrane structure design, laying of insulation layers, and other schemes are applied to engineering practice. However, there is still a gap in the relevant research of thermal environment monitoring and analysis. In order to study the thermal environment of the double-layer PTFE (polytetrafluoroethylene)-aerogel roof, multi-point thermometers were uniformly arranged to monitor, and the overall temperature field was constructed using the measured data. A thermophysical model which could accurately reflect the change of temperature field was established, whose average error was less than 5%. With the laying of roof insulation layer as the variable, three working conditions, i.e., no insulation layer, only rock wool insulation layer, and all aerogel insulation layer were constructed based on the model. The comparison indicates that the laying of aerogel reduces the average temperature of indoor space by 2.0 ℃, the original working condition has the best thermal insulation effect, and the average temperature difference between indoor and outdoor is 9.6 ℃. This paper can provide reference for the thermal insulation design of membrane structure roofs.

When encountering heavy rain, the membrane surface with a relatively small slope is easy to accumulate water. Accurately simulating the deformation of the membrane surface at this time will help ensure the safety of the structure. The linear constitutive model of membrane material used in existing research is not suitable for simulating the deformation of membrane structure in rainstorm. This study conducts a uniform load test on a membrane structure to simulate the mechanical behavior of the membrane surface in rainstorm and obtains the deformation form of the structure when water is accumulated. The linear constitutive model and the double broken line constitutive model of membrane material are used in the finite element model of the membrane structure for load analysis. By comparing the deformation of the finite element model and the actual structure, it selects the constitutive model suitable for simulating the deformation of the membrane structure in heavy rain. The numerical simulation results show that the structural deformation simulated by the double broken line constitutive model is closer to the deformation measured in the experiment than the linear constitutive model. The research results can provide a reference for the selection of the membrane constitutive model and the analysis of membrane structure in rainstorm.

DNA methylation of the ELOVL2 (Elongation Of Very Long Chain Fatty Acids-Like 2) promoter is one of the most robust molecular biomarkers for chronological age, but whether ELOVL2 plays a functional role in aging has not been explored. ELOVL2 encodes a transmembrane protein involved in the synthesis of very long polyunsaturated fatty acids (VLC-PUFAs). These fatty acids play important roles in retinal biology and photoreceptor renewal, key processes implicated in age-related eye diseases such as age-related macular degeneration (AMD). Here, we summarize our work deciphering the role of ELOVL2 in the eye emphasizing the potential functional role of age-related DNA methylation in the pathophysiology of AMD.

The design of suitable and efficient membranes for fuel and electrolyzer cells has been a long standing problem that still seeks for technical improvements. The structural properties of proton conducting poly [2,2-(m-phenylene)-5,5-bibenzimidazole] (PBI) membranes doped with phosphoric acid as electrolyte have been investigated prior and after thermal treatment by small angle neutron scattering (SANS), neutron diffraction with polarization analysis and transmission electron microscopy (TEM). Our observations indicate that during the first heating attempt stable crystalline domains in the PBI are formed and they persist in all further thermal cycles typical for electrolyte membrane operation. The combination of real space TEM picture under cryogenic conditions and reciprocal space scattering pattern with enhanced contrast by partial deuteration allows obtaining unique insight into the structure of proton conducting membranes, i.e., characteristic distances associated with irreversible changes as well as volume fraction of the different phases of the sample.

The author first describes his childhood in the South and the ways in which it fostered the values he has espoused throughout his life, his development of a keen fascination with science, and the influences that supported his progress toward higher education. His experiences in ROTC as a student, followed by two years in the US Army during the Vietnam War, honed his leadership skills. The bulk of the autobiography is a chronological journey through his scientific career, beginning with arrival at the University of California, Irvine in 1972, with an emphasis on the postdoctoral students and colleagues who have contributed substantially to each phase of his lab's progress. White's fundamental findings played a key role in the development of membrane biophysics, helping establish it as fertile ground for research. A story gradually unfolds that reveals the deeply collaborative and painstakingly executed work necessary for a successful career in science. [ABSTRACT FROM AUTHOR]

[Display omitted] • This study provides new insights into the mechanism by which antifreeze peptides regulate cell physiological functions and apoptosis under freezing stress. • A novel interaction model between frozen cells and ice crystals with antifreeze peptide intervention was revealed. • This study provides a scientific basis for the use of an antifreeze peptide as an effective cryopreservation agent for LAB and other microbes. Antifreeze peptides regulate the physiological functions of frozen cells and even their apoptosis; however, the mechanisms by which antifreeze peptides regulate these processes remain unclear, although the interactions between cell membranes and ice are well known to be important in this process. Our study aims to investigate how antifreeze peptides regulate cell physiological functions during the freezing process. We investigated the cryoprotective effect of rsfAFP on the physiological functions of S. thermophilus under freezing stress by measuring cellular metabolism activity, intracellular enzyme activity, cell membrane characterization, and cell apoptosis. The mechanism by which rsfAFP impacts S. thermophilus physiological functions under freezing stress was investigated using multispectral techniques and cryo-TEM. We show that a recombinant antifreeze peptide (rsfAFP) interacts with the extracellular capsular polysaccharides and peptidoglycan of Streptococcus thermophilus and ice to cover the outer layer of the membrane, forming a dense protective layer that regulates the molecular structure of extracellular ice crystals, which results in reduced extracellular membrane damage, depressed apoptosis and increased intracellular metabolic activity. This interaction mechanism was indicated by the fact that S. thermophilus better maintained its permeability barrier, membrane fluidity, membrane structural integrity, and cytoplasmic membrane potential during freezing stress with rsfAFP treatment. These results provide new insights into the mechanism by which rsfAFP regulates frozen cell physiological functions and apoptosis under freezing stress. [ABSTRACT FROM AUTHOR]

Due to their lightness, the capacity to adapt to the flow conditions, and the safety when operating near humans, the use of membrane-resistant structures has increased in fields as micro aerial vehicles and yachts sails. This work focuses on the computational methodology required for simulating the aeroelastic coupling of the structure with the incident wind flow. A semi-monocoque structure (composed of a main spar, a set of ribs, and an external membrane) inside a wind tunnel is simulated using two different methodologies. Firstly, a complete fluid-structure interaction is calculated by combining the finite element methodology for the solid and the unsteady Reynolds average Navier-Stokes computational fluid dynamics for the air, including nonlinear effects and prestress. Then, a low-fidelity model is applied, obtaining the linear aeroelastic eigenvalues and the temporal response of the wing. Both methodologies results are in agreement with estimating the transient mean deformation and flutter velocity. However, the modal analysis tends to overestimate the aeroelastic effects, as it calculates potential aerodynamics, predicting an instability velocity lower than that provided by the transient simulations. • Development and validation of a low-fidelity model for aeroelastic simulation of flexible membrane wings. • Analysis of the shell behavior of the membrane and limitations of the low-fidelity model. • Analysis of the effects of prestressing the membrane. • Prediction of aeroelastic instabilities through the low-fidelity model. • Analysis of the interaction between the membrane distortion and the aeroelastic behavior of the wing. [ABSTRACT FROM AUTHOR]

Membrane technology plays a vital role in drinking water and wastewater treatments. Among a number of factors affecting membrane performance, temperature is one of the dominant factors determining membrane performance. In this review, the impact of temperature on membrane structure, fouling, chemical cleaning, and membrane performance is reviewed and discussed with a particular focus on cold temperature effects. The findings from the literature suggest that cold temperatures have detrimental impacts on membrane structure, fouling, and chemical cleaning, and thus could negatively affect membrane filtration operations and performance, while warm and hot temperatures might expand membrane pores, increase membrane flux, improve membrane chemical cleaning efficiency, and interfere with biological processes in membrane bioreactors. The research gaps, challenges, and directions of temperature effects are identified and discussed indepth. Future studies focusing on the impact of temperature on membrane processes used in water and wastewater treatment and the development of methods that could reduce the adverse effect of temperature on membrane operations are needed.

Copyright of Journal of Shanghai Jiao Tong University (1006-2467) is the property of Journal of Shanghai Jiao Tong University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Shanghai Jiao Tong University (1006-2467) is the property of Journal of Shanghai Jiao Tong University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Wave-based active vibration control of a membrane structure by using the Active Sink Method is studied in this paper. Unlike the modal-based vibration control method which attempts to suppress several vibration modes that have already been excited, wave-based active controller can keep vibration modes inactive by stopping the formation of standing waves in the structure. First, the wave transfer matrix is deduced to characterize the wave transmission in the membrane structure. Then, feedforward wave control laws are derived analytically to absorb reflected waves or eliminate transmitted waves. The validity of the proposed active wave controllers is verified through numerical simulations. Simulation results show that by using the active wave controllers no standing waves will be produced in the structure, and the vibration of the membrane structure is suppressed significantly. [ABSTRACT FROM AUTHOR]

Membranes are molecular interfaces that compartmentalize cells to control the flow of nutrients and information. These functions are facilitated by diverse collections of lipids, nearly all of which are distributed asymmetrically between the two bilayer leaflets. Most models of biomembrane structure and function often include the implicit assumption that these leaflets have similar abundances of phospholipids. Here, we show that this assumption is generally invalid and investigate the consequences of lipid abundance imbalances in mammalian plasma membranes (PM). Using quantitative lipidomics, we discovered that cytoplasmic leaflets of human erythrocyte membranes have >50% overabundance of phospholipids compared to exoplasmic leaflets. This imbalance is enabled by an asymmetric interleaflet distribution of cholesterol, which regulates cellular cholesterol homeostasis. These features produce unique functional characteristics, including low PM permeability and resting tension in the cytoplasmic leaflet that regulates protein localization. These largely overlooked aspects of membrane asymmetry represent an evolution of classic paradigms of biomembrane structure and physiology., Competing Interests: Declaration of interests: The authors declare no competing interests.

Myelin is a natural and dynamic multilamellar membrane structure that continues to be of significant biological and neurological interest, especially with respect to its biosynthesis and assembly during its normal formation, maintenance, and pathological breakdown. To explore the usefulness of neutron diffraction in the structural analysis of myelin, we investigated the use of in vivo labeling by metabolically incorporating non-toxic levels of deuterium (2H; D) via drinking water into a pregnant dam (D-dam) and her developing embryos. All of the mice were sacrificed when the pups (D-pups) were 55 days old. Myelinated sciatic nerves were dissected, fixed in glutaraldehyde and examined by neutron diffraction. Parallel samples that were unfixed (trigeminal nerves) were frozen for mass spectrometry (MS). The diffraction patterns of the nerves from deuterium-fed mice (D-mice) vs. the controls (H-mice) had major differences in the intensities of the Bragg peaks but no appreciable differences in myelin periodicity. Neutron scattering density profiles showed an appreciable increase in density at the center of the lipid-rich membrane bilayer. This increase was greater in D-pups than in D-dam, and its localization was consistent with deuteration of lipid hydrocarbon, which predominates over transmembrane protein in myelin. MS analysis of the lipids isolated from the trigeminal nerves demonstrated that in the pups the percentage of lipids that had one or more deuterium atoms was uniformly high across lipid species (97.6% ​± ​2.0%), whereas in the mother the lipids were substantially less deuterated (60.6% ​± ​26.4%) with levels varying among lipid species and subspecies. The mass distribution pattern of deuterium-containing isotopologues indicated the fraction (in %) of each lipid (sub-)species having one or more deuteriums incorporated: in the D-pups, the pattern was always bell-shaped, and the average number of D atoms ranged from a low of ∼4 in fatty acid to a high of ∼9 in cerebroside. By contrast, in D-dam most lipids had more complex, overlapping distributions that were weighted toward a lower average number of deuteriums, which ranged from a low of ∼3–4 in fatty acid and in one species of sulfatide to a high of 6–7 in cerebroside and sphingomyelin. The consistently high level of deuteration in D-pups can be attributed to their de novo lipogenesis during gestation and rapid, postnatal myelination. The widely varying levels of deuteration in D-dam, by contrast, likely depends on the relative metabolic stability of the particular lipid species during myelin maintenance. Our current findings demonstrate that stably-incorporated D label can be detected and localized using neutron diffraction in a complex tissue such as myelin; and moreover, that MS can be used to screen a broad range of deuterated lipid species to monitor differential rates of lipid turnover. In addition to helping to develop a comprehensive understanding of the de novo synthesis and turnover of specific lipids in normal and abnormal myelin, our results also suggest application to studies on myelin proteins (which constitute only 20–30% by dry mass of the myelin, vs. 70–80% for lipid), as well as more broadly to the molecular constituents of other biological tissues.

Rotenone is a widely used botanical insecticide, which can inhibit the mitochondrial respiratory chain of various insect cells, while the mechanism of its toxicity to insect cells needs further investigation. The purpose of this study was to understand the toxicity level of low (0.2 µg/mL) and high (20 µg/mL) concentrations of rotenone in Spodoptera litura cells (SL-1) using trypan blue and Coomassie brilliant blue staining assays. Our study found that rotenone possessed cytotoxicity against SL-1 cells with varying effects of action between high and low concentrations. After low-concentration rotenone treatment, the SL-1 cells showed obvious aggregation time-dependently, with the fastest aggregation rate observed under the first 8 h of treatment time, but no such phenomenon was observed at high concentration. Furthermore, this aggregation phenomenon caused SL-1 cells to squeeze each other and led to the destruction of the cell membrane structure and function. Taken together, the results suggested that treatment with a low concentration of rotenone exhibited a chronic toxic effect that was significantly different from treatment with a high concentration of rotenone, which provides new insight into the cytotoxic mechanism of rotenone. [ABSTRACT FROM AUTHOR]

The pretension of building membrane structures may relax over its service lifetime, which may cause engineering failure under external loads. Therefore, the pretension of building membrane structures should be monitored or estimated regularly to compare the actual pretension to its design pretension and then to adopt some strengthening measures to mitigate future problems. Based on the geometrically nonlinear vibration of a rectangular orthotropic membrane structure, a nondestructive detection method for monitoring its pretension is developed in this article. This method is achieved by impacting a low-velocity pellet onto the membrane surface to generate vibration and detecting its response amplitude. Then the detected amplitude is converted into a pretension estimate via a derived formula. In addition, experiments for three kinds of conventional membrane material (Heytex H5573, Xing Yi Da, and ZZF 3010) were carried out according to the theoretical idea. The experimental results proved this method is feasible and verified the theoretical derivation is reasonable.

The inner boundary and the cristae membrane are connected by pore-like structures termed crista junctions (CJs). The MICOS complex is required for CJ formation and enriched at CJs. Here, we address the roles of the MICOS subunits Mic27 and Mic10. We observe a positive genetic interaction between Mic27 and Mic60 and deletion of Mic27 results in impaired formation of CJs and altered cristae membrane curvature. Mic27 acts in an antagonistic manner to Mic60 as it promotes oligomerization of the F1FO-ATP synthase and partially restores CJ formation in cells lacking Mic60. Mic10 impairs oligomerization of the F1FO-ATP synthase similar to Mic60. Applying complexome profiling, we observed that deletion of Mic27 destabilizes the MICOS complex but does not impair formation of a high molecular weight Mic10 subcomplex. Moreover, this Mic10 subcomplex comigrates with the dimeric F1FO-ATP synthase in a Mic27-independent manner. Further, we observed a chemical crosslink of Mic10 to Mic27 and of Mic10 to the F1FO-ATP synthase subunit e. We corroborate the physical interaction of the MICOS complex and the F1FO-ATP synthase. We propose a model in which part of the F1FO-ATP synthase is linked to the MICOS complex via Mic10 and Mic27 and by that is regulating CJ formation.

The existing large inflatable membrane structure is complex in structure and takes a long time to be extended. In this paper, an air-ribbed skeleton membrane structure is proposed to meet the requirement of camp and emergency tents needing to be set up quickly and easily. Ten single-arch air ribs are arranged side by side to support the tarpaulin, and wind ropes are added to the end and side faces of the tarpaulin to remain stable. The finite element model of the air-ribbed skeleton membrane structure is established to analyze the stress and the displacement of the membrane structure under combined wind and snow loads. The maximum displacement (439.4 mm) and maximum stress (29.94 MPa) are both within the safe standard. The stress and the displacement of the membrane structure in the wind load case is affected by the angle of the wind. The value of maximum stress and displacement at the wind angle of 90° are both lower than those at 0° and 45° in the wind load case. It is advisable to align the site layout of the membrane structure at the wind angle of 90°. The effect of the angle of wind ropes on the stress and displacement of the membrane structure is also studied. The maximum stress and displacement in the case when the angle of wind rope is 30° is smaller than those in the case when the angle of wind ropes is 45°. It is recommended that the wind rope should be laid at 30° to reinforce the membrane structure.

Fault diagnostics present a crucial technical issue in the areas of both the condition monitoring of machines and the monitoring of structural health. The identification of faults at an early stage in their development has an immense effect on the safety of monitored structures. Correct identification allows for the monitoring of the development of faults and the choosing of optimal operation strategies. This article discusses a method of monitoring structural health, based on the application of the Hilbert transforms (GHT and FrHT) for detecting fault formations and their development in membrane structures. A signal resulting from the HT is analyzed using spectral analysis to identify features indicating the technical state. [ABSTRACT FROM AUTHOR]

The Fluid–Mosaic Model has been the accepted general or basic model for biomembrane structure and organization for the last 50 years. In order to establish a basic model for biomembranes, some general principles had to be established, such as thermodynamic assumptions, various molecular interactions, component dynamics, macromolecular organization and other features. Previous researchers placed most membrane proteins on the exterior and interior surfaces of lipid bilayers to form trimolecular structures or as lipoprotein units arranged as modular sheets. Such membrane models were structurally and thermodynamically unsound and did not allow independent lipid and protein lateral movements. The Fluid–Mosaic Membrane Model was the only model that accounted for these and other characteristics, such as membrane asymmetry, variable lateral movements of membrane components, cis- and transmembrane linkages and dynamic associations of membrane components into multimolecular complexes. The original version of the Fluid–Mosaic Membrane Model was never proposed as the ultimate molecular description of all biomembranes, but it did provide a basic framework for nanometer-scale biomembrane organization and dynamics. Because this model was based on available 1960s-era data, it could not explain all of the properties of various biomembranes discovered in subsequent years. However, the fundamental organizational and dynamic aspects of this model remain relevant to this day. After the first generation of this model was published, additional data on various structures associated with membranes were included, resulting in the addition of membrane-associated cytoskeletal, extracellular matrix and other structures, specialized lipid–lipid and lipid–protein domains, and other configurations that can affect membrane dynamics. The presence of such specialized membrane domains has significantly reduced the extent of the fluid lipid membrane matrix as first proposed, and biomembranes are now considered to be less fluid and more mosaic with some fluid areas, rather than a fluid matrix with predominantly mobile components. However, the fluid–lipid matrix regions remain very important in biomembranes, especially those involved in the binding and release of membrane lipid vesicles and the uptake of various nutrients. Membrane phospholipids can associate spontaneously to form lipid structures and vesicles that can fuse with various cellular membranes to transport lipids and other nutrients into cells and organelles and expel damaged lipids and toxic hydrophobic molecules from cells and tissues. This process and the clinical use of membrane phospholipid supplements has important implications for chronic illnesses and the support of healthy mitochondria, plasma membranes and other cellular membrane structures. [ABSTRACT FROM AUTHOR]

In urban open spaces, especially in large cities with warm climates, users are experiencing high thermal loads, which causes thermal discomfort. Thermal comfort in open spaces can be improved; by shading. The problem is shading and protecting open spaces from stresses caused by overheating of the earth's surface and environment. The importance of shade and reducing radiation in achieving thermal comfort in open urban spaces is to increase human presence, create climate change, increase comfort conditions. Recognizing factors that create shadows, such as canopies and their characteristics, can create a favorable space to enjoy the capabilities of outdoor space. New membranes have many characteristics of nomadic tents, and, due to creating shade and natural ventilation, are very suitable for areas with hot climates. Introducing an optimal model and dimensions of a lightweight membrane canopy can create outdoor thermal comfort and increase the efficiency of outdoor spaces. In this paper, library, field, and simulation studies have been used. According to field studies, the presence of membrane canopy can cause temperature differences up to 7.8 C. The simulation results with Ansys, ENVI-met, and Ladybug showed that the membrane canopy cools the space below and prevents overheating. Between four canopy models, the saddle canopy is suitable with a 40.63% impact on the environment and creates cooler space under the canopy. Therefore, a lightweight saddle membrane canopy with dimensions of 5*5 m^2 and a useful height of 3 meters is introduced as a suitable model of membrane canopy for the hot climate of Semnan.

[Display omitted] Continuous flow injection is a key technology for serial crystallography measurements of protein crystals suspended in the lipidic cubic phase (LCP). To date, there has been little discussion in the literature regarding the impact of the injection process itself on the structure of the lipidic phase. This is despite the fact that the phase of the injection matrix is critical for the flow properties of the stream and potentially for sample stability. Here we report small-angle X-ray scattering measurements of a monoolein:water mixture during continuous delivery using a high viscosity injector. We observe both an alignment and modification of the LCP as a direct result of the injection process. The orientation of the cubic lattice with respect to the beam was estimated based on the anisotropy of the diffraction pattern and does not correspond to a single low order zone axis. The solvent fraction was also observed to impact the stability of the cubic phase during injection. In addition, depending on the distance traveled by the lipid after exiting the needle, the phase is observed to transition from a pure diamond phase (Pn 3 m) to a mixture containing both gyriod (Ia 3 d) and lamellar ( L α ) phases. Finite element modelling of the observed phase behaviour during injection indicates that the pressure exerted on the lipid stream during extrusion accounts for the variations in the phase composition of the monoolein:water mixture. [ABSTRACT FROM AUTHOR]

The polymer electrolyte membrane and its contact with electrodes has a significant effect on the performance of fuel and electrolysis cells but the choice of commercially available membranes is limited. In this study, membranes for direct methanol fuel cells (DMFCs) were made by ultrasonic spray deposition from commercial Nafion solution; the effect of the drying temperature and presence of high boiling solvents on the membrane properties was then analyzed. When choosing suitable conditions, membranes with similar conductivity, water uptake, and higher crystallinity than comparable commercial membranes can be obtained. These show similar or superior performance in DMFC operation compared to commercial Nafion 115. Furthermore, they exhibit low permeability for hydrogen, which makes them attractive for electrolysis or hydrogen fuel cells. The findings from our work will allow for the adjustment of membrane properties to the specific requirements of fuel cells or water electrolysis, as well as the inclusion of additional functional components for composite membranes.

Membrane asymmetry is critical for maintenance of several different processes such as cell signaling, apoptosis, and vesicular transport in various eukaryotic systems. Flippases of the P4-ATPase family are associated with flipping phospholipids from the luminal or exoplasmic leaflet to the cytosolic leaflet. P4-ATPases belong to the P-type ATPase family, which are activated by phosphorylation and couple ATPase activity to substrate translocation. These proteins possess a transmembrane domain responsible for substrate transport, while the cytosolic machinery performs the necessary ATP hydrolysis for this process. Several high-resolution structures of human or yeast P4-ATPases have recently been resolved, but a comprehensive overview of the changes for reaction cycle in different members was crucial for future research. In this review, we have compiled available data reflecting the reaction cycle-associated changes in conformation of P4-ATPases. Together, this will provide an improved understanding of the similarities and differences between these members, which will drive further structural, functional, and computational studies to understand the mechanisms of these flippases., Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

The plasma membrane of a biological cell is a complex assembly of lipids and membrane proteins, which tightly regulate transmembrane transport. When a cell is exposed to strong electric field, the membrane integrity becomes transiently disrupted by formation of transmembrane pores. This phenomenon termed electroporation is already utilized in many rapidly developing applications in medicine including gene therapy, cancer treatment, and treatment of cardiac arrhythmias. However, the molecular mechanisms of electroporation are not yet sufficiently well understood; in particular, it is unclear where exactly pores form in the complex organization of the plasma membrane. In this study, we combine coarse-grained molecular dynamics simulations, machine learning methods, and Bayesian survival analysis to identify how formation of pores depends on the local lipid organization. We show that pores do not form homogeneously across the membrane, but colocalize with domains that have specific features, the most important being high density of polyunsaturated lipids. We further show that knowing the lipid organization is sufficient to reliably predict poration sites with machine learning. Additionally, by analysing poration kinetics with Bayesian survival analysis we show that poration does not depend solely on local lipid arrangement, but also on membrane mechanical properties and the polarity of the electric field. Finally, we discuss how the combination of atomistic and coarse-grained molecular dynamics simulations, machine learning methods, and Bayesian survival analysis can guide the design of future experiments and help us to develop an accurate description of plasma membrane electroporation on the whole-cell level. Achieving this will allow us to shift the optimization of electroporation applications from blind trial-and-error approaches to mechanistic-driven design.

By coating the alveolar air-liquid interface, lung surfactant overwhelms surface tension forces that, otherwise, would hinder the lifetime effort of breathing. Years of research have provided a picture of how highly hydrophobic and specialized proteins in surfactant promote rapid and efficient formation of phospholipid-based complex three-dimensional films at the respiratory surface, highly stable under the demanding breathing mechanics. However, recent evidence suggests that the structure and performance of surfactant typically isolated from bronchoalveolar lung lavages may be far from that of nascent, still unused, surfactant as freshly secreted by type II pneumocytes into the alveolar airspaces. In the present work, we report the isolation of lung surfactant from human amniotic fluid (amniotic fluid surfactant, AFS) and a detailed description of its composition, structure, and surface activity in comparison to a natural surfactant (NS) purified from porcine bronchoalveolar lavages. We observe that the lipid/ protein complexes in AFS exhibit a substantially higher lipid packing and dehydration than in NS. AFS shows melting transitions at higher temperatures than NS and a conspicuous presence of nonlamellar phases. The surface activity of AFS is not only comparable with that of NS under physiologically meaningful conditions but displays significantly higher resistance to inhibition by serum or meconium, agents that inactivate surfactant in the context of severe respiratory pathologies. We propose that AFS may be the optimal model to study the molecular mechanisms sustaining pulmonary surfactant performance in health and disease, and the reference material to develop improved therapeutic surfactant preparations to treat yet unresolved respiratory pathologies. [ABSTRACT FROM AUTHOR]

Multifunctional membrane space structures have been developed in the past to realize solar arrays, array antennas, and other applications. However, the deployment of a folded membrane often requires fluctuating forces with high peaks, caused by the stiffness of the crease lines. This study proposes the use of a plain-woven textile as the base membrane for the planar deployment structures primarily because of its high packaging efficiency. The deployment forces required for the folded plain-woven textile structures to be employed in future space applications are experimentally measured. The deployment forces are compared between the proposed textile structures and the conventional film-based membranes to characterize the deployment behavior of the textile structures. Two types of experiments are conducted in this study for the one-dimensional deployment of v-folded membranes and the two-dimensional deployment of quarter Flasher origami structures, respectively. The advantages presented by the deployable textiles are as follows: (i) they require smaller and smoother deployment forces, and (ii) they are insensitive to imperfect boundary conditions. These features are important for the reliable deployment of future space structures. • Membrane structures will be compactly stored and deployed more smoothly when woven textile is used. • The attachment of thin-film devices on the textile will enable innovative space applications. • The forces for one-dimensional and two-dimensional deployment were experimentally measured. • Comparison of deployment forces between polymer film and textile clarified unique characteristics. • Extremely low shear stiffness of the plain-woven textile results in smaller deployment force and smoother deployment. [ABSTRACT FROM AUTHOR]

The plasma membrane (PM) comprises distinct subcellular domains with diverse functions that need to be dynamically coordinated with intracellular events, one of the most impactful being mitosis. The Kv2.1 voltage-gated potassium channel is conditionally localized to large PM clusters that represent specialized PM:endoplasmic reticulum membrane contact sites (PM:ER MCS), and overexpression of Kv2.1 induces more exuberant PM:ER MCS in neurons and in certain heterologous cell types. Localization of Kv2.1 at these contact sites is dynamically regulated by changes in phosphorylation at one or more sites located on its large cytoplasmic C terminus. Here, we show that Kv2.1 expressed in COS-1 cells undergoes dramatic cell cycle-dependent changes in its PM localization, having diffuse localization in interphase cells, and robust clustering during M phase. The mitosis-specific clusters of Kv2.1 are localized to PM:ER MCS, and M phase clustering of Kv2.1 induces more extensive PM:ER MCS. These cell cycle-dependent changes in Kv2.1 localization and the induction of PM:ER MCS are accompanied by increased mitotic Kv2.1 phosphorylation at several C-terminal phosphorylation sites. Phosphorylation of exogenously expressed Kv2.1 is significantly increased upon metaphase arrest in COS-1 and CHO cells, and in a pancreatic β cell line that express endogenous Kv2.1. The M phase clustering of Kv2.1 at PM:ER MCS in COS-1 cells requires the same C-terminal targeting motif needed for conditional Kv2.1 clustering in neurons. The cell cycle-dependent changes in localization and phosphorylation of Kv2.1 were not accompanied by changes in the electrophysiological properties of Kv2.1 expressed in CHO cells. Together, these results provide novel insights into the cell cycle-dependent changes in PM protein localization and phosphorylation.

Abstract Unique membrane structures endow membranes with controlled ion transport properties in both biological and artificial systems, and they have shown broad application prospects from industrial production to biological interfaces. Herein, current advances in nanochannel‐structured membranes for manipulating ion transport are reviewed from the perspective of membrane structures. First, the controllability of ion transport through ion selectivity, ion gating, ion rectification, and ion storage is introduced. Second, nanochannel‐structured membranes are highlighted according to the nanochannel dimensions, including single‐dimensional nanochannels (i.e., 1D, 2D, and 3D) functioning by the controllable geometrical parameters of 1D nanochannels, the adjustable interlayer spacing of 2D nanochannels, and the interconnected ion diffusion pathways of 3D nanochannels, and mixed‐dimensional nanochannels (i.e., 1D/1D, 1D/2D, 1D/3D, 2D/2D, 2D/3D, and 3D/3D) tuned through asymmetric factors (e.g., components, geometric parameters, and interface properties). Then, ultrathin membranes with short ion transport distances and sandwich‐like membranes with more delicate nanochannels and combination structures are reviewed, and stimulus‐responsive nanochannels are discussed. Construction methods for nanochannel‐structured membranes are briefly introduced, and a variety of applications of these membranes are summarized. Finally, future perspectives to developing nanochannel‐structured membranes with unique structures (e.g., combinations of external macro/micro/nanostructures and the internal nanochannel arrangement) for mediating ion transport are presented.

The discoloration of building facades due to airborne algae is observed in our surroundings. The growth conditions of these algae are not yet fully understood, and efficient measures for preventing the growth of the algae are not presently available. The objective of this study was to investigate the effects of the ambient environment and building structure on algal growth. A residential building in a cold region of Japan was surveyed. The roof was a multi-layered structure comprising a semi-transparent film, an air layer, and a layer of insulation from the outside, supported by rafters. The soiled state was visually observed by taking photographs. On the northeast (NE) and northwest (NW) roofs, several black stripes appeared 4 months after cleaning. The soiling increased in the spring and autumn. The soiling first appeared on the film backed by the rafter and then extended to the film backed by the air layer. The condensation time during the day in the rafter part was longer than that in the air-layer part. Condensation occurred during the night, but its frequency exhibited no dependence on the orientation of the roof. Algae tend to die when exposed to an environment with a temperature higher than 45°C. The NE roof had the shortest period with a surface temperature of >45°C. These measurements agreed well with the survey results, which indicated that the soiling mainly occurred on the NE and NW sides of the roofs. The time for algal growth was estimated under the assumption that algae can grow at surface temperatures ranging from 0 to 45°C, in agreement with the observed soiling. The observed soiling changes were well explained by the algal population calculated via a growth predictive model according to the algal temperature and relative humidity. [ABSTRACT FROM AUTHOR]

Residual plasmin activity in whole ultra-instantaneous UHT (UI-UHT) milk causes rapid fat rise during storage, seriously affecting consumers' purchase intentions. In this work, the molecular mechanisms underlying fat destabilization in whole UI-UHT milk by added plasmin were investigated based on the hydrolysis behavior of interfacial proteins. By using SDS-PAGE and peptidomic analysis, we found that the hydrolysis of interfacial proteins by plasmin led to a decrease in the amount and coverage of interfacial proteins and an increase in zeta-potential value, causing the flocculation and coalescence of fat globules. Moreover, the hydrolysis pattern varied in different categories of interfacial proteins by plasmin. In total, 125 peptides in all samples were identified. Plasmin tended to hydrolyze most major milk fat globule membrane (MFGM) proteins into protein fragments (>10 kDa) rather than peptides (<10 kDa). In contrast, peptides derived from caseins were more preferentially identified within a relatively short incubation time. It was the co-hydrolysis of caseins and some major MFGM proteins as anchors that destroyed the stability of MFGM. Furthermore, studies on the effect of trilayer membrane structure remaining at the interface on the hydrolysis rate of major MFGM proteins by plasmin revealed that ADPH and BTN were very sensitive to plasmin action, while PAS 7 was very resistant to plasmin action. Overall, membrane structure reduced the susceptibility of some major MFGM proteins to plasmin and provided protective effects. Therefore, this study provided important insights into the hydrolysis behavior of interfacial proteins in whole UI-UHT milk induced by plasmin. • Plasmin tended to hydrolyze most major MFMG proteins into protein fragments. • The relationships between interfacial behavior and fat stability were revealed. • At the interface, ADPH and BTN were very sensitive to the action of plasmin. • Membrane structure reduced the susceptibility of major MFGM proteins to plasmin. • PAS 7 was more resistant to plasmin than other major MFGM proteins. [ABSTRACT FROM AUTHOR]