1. Antigenic Proteins Involved in Occupational Rhinitis and Asthma Caused by Obeche Wood (Triplochiton Scleroxylon)
- Author
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Inmaculada Doña, Arantxa Palacín, Araceli Díaz-Perales, Ana Aranda, Cristina Gomez-Casado, Paloma Campo, Miguel Blanca, Luisa Galindo, [Aranda,A] Allergy Research Laboratory, Carlos Haya Hospital, Málaga, Spain. [Campo,P, Doña,I, Galindo,L, Blanca,M] Unidad de Gestión Clínica Allergy, Carlos Haya Hospital, Málaga, Spain. [Palacin,A, Gomez-Casado,C, Díaz-Perales,A] Biotechnology Department, Center for Plant Biotechnology and Genomics, Pozuelo de Alarcon, Madrid, Spain., Ministry of Health grant FIS/PI071175, Andalusian Health Division grants PI-0182/2008, grant from the Spanish Society of Allergy, Ministry of Science and Innovation-DGI, Spain (grant BIO2009-07050), and and Thematic Network FIS-RIRAAF (RD07/0064).
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Male ,Triplochiton scleroxylon ,Allergy ,Pulmonology ,Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Globulins::Serum Globulins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin E [Medical Subject Headings] ,lcsh:Medicine ,Named Groups::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings] ,Plant Science ,Biochemistry ,Trees ,Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] ,Diseases::Occupational Diseases [Medical Subject Headings] ,0302 clinical medicine ,Alérgenos ,Anatomy::Plant Structures::Xylem::Wood [Medical Subject Headings] ,Medicine ,lcsh:Science ,Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Plant Proteins [Medical Subject Headings] ,Malvaceae ,Rinitis ,Plant Proteins ,Rhinitis ,0303 health sciences ,Multidisciplinary ,Allergy and Hypersensitivity ,Middle Aged ,Plants ,Occupational and Industrial Health ,Diseases::Respiratory Tract Diseases::Nose Diseases::Rhinitis [Medical Subject Headings] ,Wood ,Madera ,3. Good health ,Occupational Diseases ,Electroforesis en gel de poliacrilamida ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunoassay::Immunoenzyme Techniques::Enzyme-Linked Immunosorbent Assay [Medical Subject Headings] ,Named Groups::Persons::Age Groups::Adolescent [Medical Subject Headings] ,visual_art ,Plant biochemistry ,Diseases::Respiratory Tract Diseases::Respiratory Hypersensitivity::Asthma [Medical Subject Headings] ,visual_art.visual_art_medium ,Electrophoresis, Polyacrylamide Gel ,Female ,Exposición Profesional ,Public Health ,Occupational exposure ,Enfermedades profesionales ,Occupational asthma ,Research Article ,Adult ,Named Groups::Persons::Age Groups::Adult::Young Adult [Medical Subject Headings] ,Adolescent ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Electrochemical Techniques::Electrophoresis::Electrophoresis, Polyacrylamide Gel [Medical Subject Headings] ,Immunology ,Check Tags::Male [Medical Subject Headings] ,Immunoglobulins ,Enzyme-Linked Immunosorbent Assay ,Environmental and Occupational Lung Diseases ,complex mixtures ,Young Adult ,03 medical and health sciences ,Occupational Exposure ,Named Groups::Persons::Age Groups::Adult [Medical Subject Headings] ,Humans ,Biology ,Asma ,Skin Tests ,030304 developmental biology ,Asthma ,Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Globulins::Serum Globulins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin G [Medical Subject Headings] ,Enzimoinmunoanálisis por adsorción ,business.industry ,lcsh:R ,Proteins ,Health Care::Environment and Public Health::Public Health::Environmental Pollution::Environmental Exposure::Occupational Exposure [Medical Subject Headings] ,Allergens ,Immunoglobulin E ,medicine.disease ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Immunologic Tests::Skin Tests [Medical Subject Headings] ,respiratory tract diseases ,Check Tags::Female [Medical Subject Headings] ,030228 respiratory system ,Immunoglobulin G ,lcsh:Q ,Clinical Immunology ,Chemicals and Drugs::Biological Factors::Antigens::Allergens [Medical Subject Headings] ,Organisms::Eukaryota::Plants::Viridiplantae::Streptophyta::Embryophyta::Angiosperms::Malvaceae [Medical Subject Headings] ,business - Abstract
Journal Article; Research Support, Non-U.S. Gov't; BACKGROUND Obeche wood dust is a known cause of occupational asthma where an IgE-mediated mechanism has been demonstrated. OBJECTIVE To characterize the allergenic profile of obeche wood dust and evaluate the reactivity of the proteins by in vitro, ex vivo and in vivo assays in carpenters with confirmed rhinitis and/or asthma MATERIALS AND METHODS An in-house obeche extract was obtained, and two IgE binding bands were purified (24 and 12 kDa) and sequenced by N-terminal identity. Specific IgE and IgG, basophil activation tests and skin prick tests (SPTs) were performed with whole extract and purified proteins. CCD binding was analyzed by ELISA inhibition studies. RESULTS Sixty-two subjects participated: 12 with confirmed occupational asthma/rhinitis (ORA+), 40 asymptomatic exposed (ORA-), and 10 controls. Of the confirmed subjects, 83% had a positive SPT to obeche. There was a 100% recognition by ELISA in symptomatic subjects vs. 30% and 10% in asymptomatic exposed subjects and controls respectively (p
- Published
- 2013
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