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8 results on '"Msuthwana, Petunia"'

2. Additional file 1 of Regulation of feather follicle development and Msx2 gene SNP degradation in Hungarian white goose

Author

Song, Yupu, Liu, Chang, Zhou, Yuxuan, Lin, Guangyu, Xu, Chenguang, Msuthwana, Petunia, Wang, Sihui, Ma, Jingyun, Zhuang, Fangming, Fu, Xianou, Wang, Yudong, Liu, Tuoya, Liu, Qianyan, Wang, Jingbo, Sui, Yujian, and Sun, Yongfeng

Abstract

Additional file 1. The agarose gel electrophoresis diagram of the construction of the overexpression vector is shown in the fig. S1.

Published
2023
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3. In ovo injection of CHIR-99021 promotes feather follicles development via activating Wnt/β-catenin signaling pathway during chick embryonic period

Author

Feng, Ziqiang, primary, Mabrouk, Ichraf, additional, Msuthwana, Petunia, additional, Zhou, Yuxuan, additional, Song, Yupu, additional, Gong, Haizhou, additional, Li, Shengyi, additional, Min, Changguo, additional, Ju, Anqi, additional, Duan, Aoyi, additional, Niu, Jiangting, additional, Fu, Jinhong, additional, Yan, Xiaomin, additional, Xu, Xiaohui, additional, Li, Chuanghang, additional, and Sun, Yongfeng, additional

Published
2022
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4. Characterization of Embryonic Skin Transcriptome in Anser cygnoides at Three Feather Follicles Developmental Stages

Author

Liu, Chang, primary, Sello, Cornelius Tlotliso, additional, Sui, Yujian, additional, Hu, Jingtao, additional, Chen, Shaokang, additional, Msuthwana, Petunia, additional, Zhou, Yuxuan, additional, Wachiebine, Sulleyman Kassim, additional, Sun, Yue, additional, Liu, Jing, additional, Li, Shengyi, additional, Yang, Wei, additional, Song, Yupu, additional, Xu, Yunpeng, additional, Guo, Chanying, additional, Sui, Qihui, additional, and Sun, Yongfeng, additional

Published
2020
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5. Variations in the Expression Pattern of HSP27 and MSK1 Genes During the Development of Prehierarchical Follicles in the Zi Geese (Anser Cygnoides)

Author

Sello, Cornelius Tlotliso, primary, Liu, Chang, additional, Lu, Hongtao, additional, Wang, Ziqiu, additional, Msuthwana, Petunia, additional, Tyasi, Thobela Louis, additional, Sun, Yue, additional, Liu, Jing, additional, Xu, Chenguang, additional, Zhou, Yuxuan, additional, Li, Shengyi, additional, Yang, Hui, additional, Sui, Yujian, additional, Hu, Jingtao, additional, and Sun, Yongfeng, additional

Published
2020
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6. De Novo Assembly and Comparative Transcriptome Profiling of Anser anser and Anser cygnoides Geese Species’ Embryonic Skin Feather Follicles

Author

Sello, Cornelius Tlotliso, primary, Liu, Chang, additional, Sun, Yongfeng, additional, Msuthwana, Petunia, additional, Hu, Jingtao, additional, Sui, Yujian, additional, Chen, Shaokang, additional, Zhou, Yuxuan, additional, Lu, Hongtao, additional, Xu, Chenguang, additional, Sun, Yue, additional, Liu, Jing, additional, Li, Shengyi, additional, and Yang, Wei, additional

Published
2019
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7. Variations in the Expression Pattern of HSP27and MSK1Genes During the Development of Prehierarchical Follicles in the Zi Geese (Anser Cygnoides)

Author

Sello, Cornelius Tlotliso, Liu, Chang, Lu, Hongtao, Wang, Ziqiu, Msuthwana, Petunia, Tyasi, Thobela Louis, Sun, Yue, Liu, Jing, Xu, Chenguang, Zhou, Yuxuan, Li, Shengyi, Yang, Hui, Sui, Yujian, Hu, Jingtao, and Sun, Yongfeng

Abstract

The p38MAPKs (mitogen-activated protein kinases) signaling contributes a pivotal role in mammalian ovarian follicular development; however, the knowledge regarding their expression in geese remains unresolved. The objective of the current study was to determine the spatio-temporal expression of heat shock protein 27 (HSP27)and mitogen- and stress-activated protein kinase 1 (MSK1) genes in the prehierarchical follicles during geese ovarian development. The prehierarchical follicles samples were harvested from 35- to 37-week-old healthy laying geese. HSP27and MSK1relative expression in various sized prehierachical follicles was detected by real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting. Follicular wall localization of HSP27and MSK1was examined by using immunohistochemistry. Our results at mRNA level indicated that HSP27was highly expressed in middle white follicles whereas MSK1was predominantly expressed in small white follicles. The western blotting results for HSP27and MSK1were inconsistent with the RT-qPCR results in various stages of prehierachical follicular development but noticeably, HSP27proteins were still expressed more in middle white follicles while MSK1proteins were more abundant in primary follicles. At different stages of prehierarchical development, immunodetections in the granulosa and theca cells revealed that HSP27was intensively localized in middle white follicles while strong detections of MSK1were observed in large white follicles. These results indicate HSP27and MSK1might be associated to the key regulators of folliculogenesis in geese.

Published
2020
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8. Characterization of Embryonic Skin Transcriptome in Anser cygnoides at Three Feather Follicles Developmental Stages.

Author

Chang Liu, Sello, Cornelius Tlotliso, Yujian Sui, Jingtao Hu, Shaokang Chen, Msuthwana, Petunia, Yuxuan Zhou, Wachiebine, Sulleyman Kassim, Yue Sun, Jing Liu, Shengyi Li, Wei Yang, Yupu Song, Yunpeng Xu, Chanying Guo, Qihui Sui, and Yongfeng Sun

Subjects
*BONE morphogenetic protein receptors, *FIBROBLAST growth factors, *FIBROBLAST growth factor 2, *BONE morphogenetic proteins, *MITOGEN-activated protein kinases, *FEATHERS, *GENE expression profiling
Abstract

In order to enrich the Anser cygnoides genome and identify the gene expression profiles of primary and secondary feather follicles development, de novo transcriptome assembly of skin tissues was established by analyzing three developmental stages at embryonic day 14, 18, and 28 (E14, E18, E28). Sequencing output generated 436,730,608 clean reads from nine libraries and de novo assembled into 56,301 unigenes. There were 2,298, 9,423 and 12,559 unigenes showing differential expression in three stages respectively. Furthermore, differentially expressed genes (DEGs) were functionally classified according to genes ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and series-cluster analysis. Relevant specific GO terms such as epithelium development, regulation of keratinocyte proliferation, morphogenesis of an epithelium were identified. In all, 15,144 DEGs were clustered into eight profiles with distinct expression patterns and 2,424 DEGs were assigned to 198 KEGG pathways. Skin development related pathways (mitogen-activated protein kinase signaling pathway, extra-cellular matrix -receptor interaction, Wingless-type signaling pathway) and genes (delta like canonical Notch ligand 1, fibroblast growth factor 2, Snail family transcriptional repressor 2, bone morphogenetic protein 6, polo like kinase 1) were identified, and eight DEGs were selected to verify the reliability of transcriptome results by real-time quantitative PCR. The findings of this study will provide the key insights into the complicated molecular mechanism and breeding techniques underlying the developmental characteristics of skin and feather follicles in Anser cygnoides. [ABSTRACT FROM AUTHOR]

Published
2020
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