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Abstract Multiple-input multiple-output (MIMO) orthogonal frequency division multiplexing (OFDM) with index modulation (IM) MIMO-OFDM-IM is a modulation technique that has garnered significant interest in recent times owing to its attraction of smooth transition to greener communications, enhancing efficiency in terms of energy, spectrum and compatibility to previous existing standards without the need for drastic changes in the physical layer. It is also proved that even though OFDM-IM provides better immunity to carrier frequency offset compared to traditional OFDM, the sensitivity to frequency error is still a major issue and it has to be resolved on priority basis to fully exploit the potential offered by the IM technique. In this work, we propose a novel non-data-aided algorithm that efficiently estimates and eliminates the offset present in the received signal. The algorithm has been analyzed for the MIMO-OFDM-IMbased wireless local area network system using the high throughput task group (TGn) MIMO channel models in the presence of AWGN. In addition, it is compared with the other popular blind algorithms based on both pilots and inactive data tones. The simulation plots depict a clear improvement of the new estimator over the existing methods not only in the low signal-to-noise ratio (SNR) regions but also in the high SNR region.

Background and Aims: Pneumoperitoneum creation for laparoscopic surgery increases the intraabdominal pressure and causes alveolar atelectasis. We investigated the influence of an increase in intra-abdominal pressure (IAP) on ventilatory mechanical power (MP) delivery during pneumoperitoneum creation for laparoscopic cholecystectomy. Material and Methods: In a prospective cohort design, we enrolled 42 patients undergoing laparoscopic cholecystectomy. During pneumoperitoneum creation, the IAP was sequentially raised to three predefined IAP levels (8, 11 and 14 mmHg), keeping identical ventilatory settings (timepoints T1, T2, and T3). After that, positive end-expiratory pressure (PEEP) was sequentially raised from 5 to 8 to 11 cmH2O (timepoint T4 and T5). The primary outcome included ventilatory MP delivery at each timepoint. Other variables included respiratory driving pressure (DP), airway resistance (AR), and respiratory compliance (RC). Results: The MP increased linearly with a rise in IAP from T1 to T3 (r = 0.71, P < 0.001); the MP increased by 0.19 per unit rise in IAP (effect size 0.90, P < 0.001). A similar positive correlation was also observed between DP and IAP from T1 to T3 (r = 0.73, P < 0.001); the DP increased by 0.72 per unit rise in IAP (effect size 0.89, P < 0.001). The MP increased significantly on increasing PEEP from T3 to T5, while the DP decreased concomitantly (P < 0.001). The AR increased significantly from T1 to T3, while RC decreased concomitantly; vice-versa was observed at T4 and T5 (P < 0.001). Conclusions: The ventilatory MP delivery rises linearly with an increase in IAP. Targeting an IAP-guided MP level could be an attractive approach to minimize lung injury.

Background: As household food insecurity is directly connected with the amount and quality of nutrition available to a person, maternal food insecurity, therefore, might have a role to play in poor pregnancy outcomes such as low birth weight (LBW). Objectives: To find out whether maternal food insecurity was associated with LBW among infants born between May and June of 2020 in a tertiary care hospital of Eastern India. Materials and Methods: The study was a case–control design, conducted among a set of sex-matched cases and controls. 105 LBW infants were taken as cases and 105 infants with birth weight ≥2500 g were taken as controls. Multivariable logistic regression analysis was done to ascertain the relationship between the independent and dependent variables and crude and adjusted odds ratios (AOR) were calculated to find out the association between the exposure and the outcome. Results: The cases and controls were statistically comparable with respect to baseline characteristics. Univariable analysis showed that the odds of LBW were 7.77 (95% confidence interval [CI]: 3.73–16.16) times more in food-insecure households. After adjusting for other variables included in the analysis, the AOR for food insecurity was 8.04 (95% CI: 3.81–16.94). Conclusion: The odds of giving birth to a LBW infant were higher in mothers belonging to food-insecure households. Addressing the prevalent barriers to accessibility, affordability, and adequacy of nutrition can be effective in mitigating this problem.

Aaptamine (8,9-dimethoxy-1H-benzo[de][1,6]naphthyridine), an alkaloid obtained from marine sponges of the genus Aaptos (Demospongiae, Suberitida, Suberitidae), has attracted significant attention as a promising scaffold for the development of antioxidant, antibacterial, and anticancer agents. This review offers an extensive overview of updated research on aaptamine, focusing on its multifaceted pharmacological properties. The antioxidant potential of aaptamine reflects its potential ability for use in the DPPH free radical scavenging assay, for suppressing ROS, and subsequently deactivating the MAPK and AP-1 signaling pathway. Moreover, it demonstrates notable antibacterial activity against pathogenic bacteria, including mycobacterial active and dormant states, making it a potential candidate for combating bacterial infections. Additionally, aaptamine shows promising anticancer activity by inhibiting cancer cell proliferation, apoptosis induction, and suppressing tumor growth through various signaling pathways, including the regulation of PTEN/PI3K/Akt and CDK2/4, and the regulation of cyclin D1/E in cell cycle arrest. The unique chemical structure of aaptamine offers opportunities for structural modifications aimed at enhancing its antioxidant, antibacterial, and anticancer activities. The exploration of aaptamine as a scaffold in the development of novel therapeutic agents offers great promise for addressing various challenges associated with oxidative stress, bacterial infections, and cancer. This article underscores the potential of aaptamine as a valuable marine-derived scaffold in the fields of antioxidant, antibacterial, and anticancer therapy.

Background This paper investigated the connection between transactional leadership styles, contingent rewards, punishments, and employee performance while emphasizing employee engagement’s mediating role. Existing research has predominantly focused on isolated associations between contingent rewards, punishment, and employee performance, leaving gaps in the empirical exploration of these mediating mechanisms. To address this research gap, our study has introduced a conceptual framework to understand the multifaceted connection between contingent rewards, punishment, and their effects on employee performance, with a specific emphasis on the mediating function of employee engagement. Methods We involved 273 full-time non-clinical healthcare professionals employed in NABH-accredited hospitals in Jharkhand, India. A structured survey instrument was employed for data collection from the specific survey participants, with the investigation of the research hypotheses conducted through the application of partial least squares-structural equation modeling (PLS-SEM). Results Preliminary findings suggested that contingent rewards and punishment do not directly influence employee performance. Instead, our study highlighted the critical mediating role of employee engagement, particularly its dimensions of Vigor, absorption, and dedication. Conclusions This research has underscored rewards and punishments as essential tools for influencing employee behaviour, motivation, and performance. Employee engagement, as a multifaceted construct, not only benefits individual employees but also significantly impacts overall organizational performance and success.

Fused Deposition Modeling (FDM) has emerged as a pivotal additive manufacturing technology that enables the creation of complex geometries with high precision and repeatability, particularly in the fabrication of biomedical implants. The functional additive manufacturing components, such as custom prosthetics, dental implants, and surgical guides, are expected to have dimensional accuracy and superior surface finish to ensure biocompatibility, osseointegration, and optimal tissue-implant interaction. This study aimed to optimize the FDM process parameters to minimize circularity error and surface roughness (Ra) using the Taguchi method, with a focus on biomedical implant fabrication. The printing temperature, printing speed, and layer thickness were identified as significant factors affecting circularity and Ra. Orthogonal arrays of Taguchi L9 were devised with three levels of each factor. Circularity and Ra were measured on the printed specimens, which included biomedical implant prototypes. The signal-to-noise (S/N) ratio response analysis determined the optimal parameter combinations. Results showed that the most dominant factor for circularity error reduction was build orientation, while layer thickness had the highest influence on Ra. The confirmation runs validated that the Taguchi-optimized parameters reduced the circularity error by 42% and Ra by 67% compared to default settings. Therefore, the Taguchi method proved effective in identifying the ideal process combinations for biomedical implant fabrication. The findings can help FDM users in the biomedical sector minimize dimensional errors and improve surface finish quality, ultimately enhancing the performance and reliability of medical implants.

Key Clinical Message Normal pressure hydrocephalus is rarely associated with Belly dancer's dyskinesia and seizure. Abstract Belly dancer's dyskinesia (BDD) is characterized by bilateral, sluggish, involuntary, repetitive, and rhythmic motions of the anterior abdominal wall. We present a rare case of a 78‐year‐old man diagnosed with normal pressure hydrocephalus associated with BDD and seizure who presented with left‐sided weakness of the body and abnormal body movements.

Introduction: The term 'patient feedback' can be measured through various formal reactions, including levels of patient comfort, experience, perspectives, and assessments of care in terms of convenience, continuity, and quality. By recording the patient’s viewpoint on the quality of care provided by a tertiary healthcare facility, it can be acknowledged through patient feedback. This data can then be utilised to improve health services and enhance the overall patient experience. Aim: To assess patient satisfaction with the services provided at a tertiary care hospital in Banda. Materials and Methods: This descriptive cross-sectional study was conducted at the government medical college in Banda, a district in the Bundelkhand region of Uttar Pradesh, a northern state of India. The study took place from December 2022 to February 2023 at the medical college and its associated hospital. A total of 400 participants who consented to be part of the study were included. Purposive sampling technique was employed for data collection. Outpatients were interviewed upon exiting the clinics, while inpatients were interviewed in the wards. Results: In the questionnaire, there was a question asking, about patient's overall satisfaction during the visit to the hospital. Since everyone’s score was higher than the 'poor' category, all categories were merged into one word, 'satisfied,' except for the 'poor' category to facilitate better understanding. The majority of patients from the Outpatient Department (OPD) (91, 45.5%) and Inpatient Department (IPD) (198, 99%) were satisfied with the attitude and communication of the doctor. Additionally, 98.5% of IPD patients were satisfied with the doctor's knowledge. A total of 99% of participants responded "yes" when questioned about their preference to return to the hospital for treatment. Conclusion: Overall, it was observed that the behaviour and care provided by hospital staff satisfied the majority of patients, who also expressed satisfaction with the indoor and outdoor services of the hospital.

Bullying is an intimidating behavior that can be internalized as a learned behavior. The Study aimed to create thoughtfulness among employees about how and what makes an individual become a bully or engage in bullying with no awareness. The Study explored the view of an employee to understand how unconscious behavior can influence one’s actions and behavior toward bullying. This Study practiced a Focus Group Discussion and conducted the discussion virtually on a sample of 31 employees of the Service and Non-Service sectors. The researcher discussed with 11 participants in one group and 20 in another on two different days. This discussion helped to provide awareness about unconsciousness in bullying behavior at the workplace. This discussion helped to gather views of working people from various sectors. It illustrated that focus groups could enhance awareness and empower one’s thoughts and beliefs. An important finding can be that the group identified the challenges of Unconscious bullying, like lack of self-awareness, marginal ethical and moral guidance, and eminence. In conclusion, it is observed from the discussion and responses from employees that there is a need to have healthy communication abilities in stating their feelings assertively.

The study investigates whether adverse conditions associated with disability stimulate entrepreneurial pursuits, emphasizing perceived discrimination in entrepreneurship. It investigates the direct effect of Entrepreneurial Competency (EC) on Entrepreneurial Intention (EI) and Entrepreneurial Behavior (EB) among Persons with Spinal Cord Injuries (PSCI) in South Karnataka. Additionally, it assesses the moderating effect of Perceived Discrimination (PD) on the EC and EB relationship. A structured questionnaire based on validated scales to measure EC, EI, EB, and PD was administered to 49 participants. Data were analyzed using Partial Least Square Structural Equation Modeling (PLS-SEM) to test the hypotheses. Results demonstrate that EC significantly enhances EI and EB, with path coefficients of 0.608 and 0.334, respectively. EI significantly influences EB with a path coefficient of 0.55. The interaction between PD and EC showed a positive relationship with EI at the 90% confidence interval (p = 0.096). The R-squared values indicated moderate predictive validity for the model. This research highlights the importance of EC in fostering EI and EB among PSCI. PD may strengthen these relationships, showing a nuanced impact that aligns with literature that suggests PD can catalyze activism and collective action among PWDs. The findings highlight the necessity of acknowledging PD’s dual role as an impediment and a potential motivator for entrepreneurial endeavors, emphasizing the imperative for tailored interventions that mitigate the negative impacts of PD and harness its potential to drive entrepreneurial innovation and resilience among PSCI.

Necrotizing enterocolitis (NEC) remains a devastating disease in preterm and term neonates. Despite significant progress made in understanding NEC pathogenesis over the last 50 years, the inability of current definitions to discriminate the various pathophysiological processes underlying NEC has led to an umbrella term that limits clinical and research progress. In this mini review, we provide a historical perspective on how NEC definitions and pathogenesis have evolved to our current understanding of NEC endotypes. We also discuss how artificial intelligence-based approaches are influencing our knowledge of risk-factors, classification and prognosis of NEC and other neonatal intestinal injury phenotypes.

The emergence of non-albicans Candida as a potential causative agent of candidiasis following Candida albicans is occurring globally. It is known that phenolic cresols, thymol, and carvacrol have anti-Candida characteristics. However, there are few papers that disclose the mechanism of action, which is characterized by a damaged cell wall and ergosterol synthesis, as well as calcium-induced death in C. albicans. This work utilized in-silico docking analysis to examine the molecular targets of thymol and carvacrol in Candida glabrata. The results were then validated in vitro by antifungal susceptibility testing and growth curve analysis. The study incorporated C. glabrata deletion mutants for the target proteins to gain understanding of the function of these proteins in the antifungal effects of the two monoterpenoids. After conducting molecular docking and in silico toxicity evaluations, thymol and carvacrol were chosen for in vitro investigations. Experimental tests conducted in a laboratory setting have shown that both phytocompounds exhibit strong binding affinity towards cell wall synthesis proteins (Kre1p, Kre2p, Ecm33p), calcium channel proteins (Mid1p, Ecm7p), and proteins involved in the ergosterol synthesis pathway (Erg5p). These proteins are likely to be the specific targets for the anti-Candida properties of the two isomeric monoterpenoids in C. glabrata. Minimum inhibitory concentration (MIC50) values for thymol and carvacrol range from 50 µg/ml to 75 µg/ml. The wild-type strains exhibit a minimum fungicidal concentration of 100 µg/ml for thymol and 125 µg/ml for carvacrol. The results underscore the significance of these proteins in the fungal reaction to thymol and carvacrol and also indicate a promising opportunity for the development of novel antifungal treatment methods utilizing these proteins.

Widespread usage of antifungals has led to the development of antifungal resistance, causing a change in the epidemiology of the responsible agents from albicans to non-Candida albicans species. Pharmaceutical repurposing is an alternate strategy that has provided a cost-effective method to address the increasing resistance to antifungal medications. The objective of this work was to examine the antifungal properties of Alexidine dihydrochloride (AXD) and Hexachlorophene (HCP) against a non-Albicans Candida model, C. glabrata. The lowest inhibitory doses of AXD and HCP against C. glabrata were determined by in vitro methods to be 0.69-1.03 µM and 14.75-19.66 µM, respectively. The minimum doses of AXD and HCP that caused fungicidal effects were defined as 1.375 µM and 61.44 µM, respectively. Three proteins involved in crucial physiological pathways, namely cell wall production (Kre1p, Kre2p, Ecm33p), membrane calcium channel (Mid1p, Ecm7p), and ergosterol biosynthesis (Erg5p), were chosen as potential targets for the medications due to their functions in survival and disease development. SWISS MODEL was used to create the 3D structures of predicted targets of C. glabrata. The quality of these structures was assessed using Ramachandran plot statistics. AXD and HCP were analyzed by docking software AutoDock Vina against these targets. The findings of computational investigations have shown that both medicines exhibit interaction affinities with all the selected protein types. The binding energy profiles of AXD and HCP showed that Mid1p had the lowest binding energies at -10.1 kcal/mol and -9.2 kcal/mol, respectively. Kre2p had binding energies of -7.9 kcal/mol and -7.1 kcal/mol, respectively. Erg5p had binding energies of -6.6 kcal/mol and -6.2 kcal/mol, respectively. Ecm7p had binding energies of -6.6 kcal/mol and -6.1 kcal/mol, respectively. Recm7p had binding energies of -4.8 kcal/mol and -7.7 kcal/mol, respectively. These results suggest that these genes are likely targets of the two drugs in C. glabrata.

Background and Objectives: Radiation therapy (RT) plays a crucial role in managing prostate cancer, offering effective disease control and improving survival rates in both localized and recurrent cases. However, RT can lead to hemorrhagic cystitis, a significant late complication resulting in chronic morbidity and other health issues. This study aims to evaluate the real-world incidence of radiation-induced hemorrhagic cystitis requiring surgical intervention. Materials and Methods: This retrospective cohort study analyzed data from prostate cancer survivors treated for hematuria at our center between January 2014 and January 2024. Patients were included if cystoscopy identified radiation cystitis as the cause of hematuria. Descriptive statistics were used, and binomial logistic regression analyses with univariate and multivariate analysis were performed to identify risk factors for worse outcomes. Results: Fifty-two patients met the inclusion criteria. The estimated cumulative incidence at a median follow-up of 5.3 years was 4.5%. Among the participants, 21.2% required more than two transurethral bladder fulguration (TUBF) procedures, and 38.5% needed more than two hospital admissions for hematuria management. The median time to the first fulguration was 64 months. Blood transfusions were necessary in 53.8% of cases, and 38.5% required hyperbaric oxygen therapy. Ultimately, 5.8% of the patients underwent cystectomy. Univariate analysis identified ischemic heart disease (IHD) and antiplatelet therapy as significant risk factors (OR: 5.17 and 5.18, respectively), along with longer time to first fulguration (OR: 5.02). Multivariate analysis confirmed antiplatelet therapy (OR: 2.8, p = 0.05) and time to first TUBF (OR: 1.8, p = 0.02) as significant predictors of multiple procedures. Conclusions: Radiation cystitis remains a significant burden on prostate cancer survivors. Patients on antithrombotic agents, those with delayed initial presentations, and those who received radiation as salvage therapy are more likely to experience higher morbidity.

This review examines ultra-low frequency (ULF) waves across different planetary environments, focusing on Earth, Mercury, and Saturn. Data from spacecraft missions (CHAMP, Swarm, and Oersted for Earth; MESSENGER for Mercury; and Cassini for Saturn) provide insights into ULF wave dynamics. At Earth, compressional ULF waves, particularly Pc3 waves, show significant power near the equator and peak around Magnetic Local Time (MLT) = 11. These waves interact complexly with Alfvén waves, impacting ionospheric responses and geomagnetic field line resonances. At Mercury, ULF waves transition from circular to linear polarization, indicating resonant interactions influenced by compressional components. MESSENGER data reveal a lower occurrence rate of ULF waves in Mercury’s foreshock compared to Earth’s, attributed to reduced backstreaming protons and lower solar wind Alfvénic Mach numbers, as ULF wave activity increases with heliocentric distance. Short Large-Amplitude Magnetic Structures (SLAMS) observed at Mercury and Saturn show distinct characteristics compared to those of Earth, including the presence of whistler precursos waves. However, due to the large differences in heliospheric distances, SLAMS (their temporal scale size correlate with the ULF wave frequency) at Mercury are significantly shorter in duration than at Earth or Saturn, since the ULF wave frequency primarily depends on the strength of the interplanetary magnetic field. This review highlights the variability of ULF waves and SLAMS across planetary environments, emphasizing Earth’s well-understood ionospheric interactions and the unique behaviours observed for Mercury and Saturn. These findings enhance our understanding of space plasma dynamics and underline the need for further research regarding planetary magnetospheres.

This research study investigates the effect of intrinsic and extrinsic motivation on employee performance, with a specific focus on the moderating role of employees' work experience. This investigation utilizes a proposed framework, focusing on higher educational institutions in West Bengal, India. It contributes to the human resource management field by comparing teacher performance in private and government academic institutions based on their motivation levels. The study employs a quantitative approach, collecting data from 250 teachers in West Bengal, India, using a structured questionnaire. The dataset underwent analysis employing Partial Least Squares Structural Equation Modeling (PLS-SEM) due to its inherent capacity to accommodate smaller sample sizes while delivering precise and insightful outcomes. The results indicate a strong positive relationship between intrinsic and extrinsic motivation and teacher performance in both types of institutions. Work experience moderates the connection between intrinsic motivation and performance in both sectors but has no significant impact on the relationship between extrinsic motivation and performance in private academic institutions. This study links a gap in the literature by empirically exploring the impact of teacher motivation on their performance and provides valuable insights into the complex interplay among motivation, work experience, and performance. Practically, it emphasizes the importance of employee motivation and accumulated work experience in enhancing performance. This study attempts to underscore the role of work experience as a moderating variable, thereby contributing to the novel discourse in the educational landscape of the post-pandemic era. The findings demand to identification of diverse organizational developmental drivers as work experience does not exhibit a strong mediation effect. However, limitations such as potential response bias should be considered in future research in this area.

The next-generation of wireless local area network systems are being conceptualized with new applications, smart devices and use cases which mandate unprecedented levels of high data rates, spectral efficiency, reliability, low latency and high energy efficiency. The index modulated orthogonal frequency division multiplexing (OFDM-IM) stands out as the most endearing candidate for physical layer modulation technique which provides a smooth transit to green communications. However, OFDM-IM being a multicarrier technique similar to classical OFDM is also very sensitive to frequency synchronization errors and needs to be addressed on priority. In this article, a novel algorithm is proposed which estimates and corrects the carrier frequency offset at the receiver and the algorithm's performance is compared with two frequency domain variants of OFDM-IM and the classical OFDM under the same channel conditions and the simulation results show that our algorithm is not only capable of meeting the standard requirement of +/-20ppm but can handle higher offsets till +/-30ppm.

Secondary metabolites, such as phenols and salicylic, play a crucial role in the regulation of development and tolerance mechanisms against a wide range of stresses. During adverse conditions such as biotic and abiotic stresses, plants induce the biosynthesis of phenolic compounds to provide tolerance. Phenolics are secondary aromatic metabolites synthesized through the shikimate/phenylpropanoid pathway or polyketide acetate/malonate pathway, which produce monomeric and polymeric phenolics. Phenolic compounds in plants not only take part in preventing stresses but also in regulating physiological activities. These compounds significantly regulate both below- and above-ground defense mechanisms. Plants synthesize thousands of phenolic compounds throughout their evolution to survive in changing environments. Environmental factors, such as high light, cold, drought, heavy metals, etc., increase the accumulation of phenolics to neutralize any toxic effects. This review focuses on the biosynthesis of phenolic compounds and their updated studies against abiotic stresses.

Background: Severe acute malnutrition (SAM) is one of the major causes of mortality and morbidity among children in developing countries and is an important contributing factor to deaths occurring from preventable causes in children

Diabetic ulcers remain a significant challenge in wound care due to loss of epithelial cell migration. Aligned nanofibrous scaffolds mimicking the skin's extracellular matrix (ECM) are promising candidates for diabetic wound healing. In this study, a composition of poly(ε-caprolactone), gelatin, and dopamine-containing varying amounts of ε-polylysine (ε-PL) was electrospun to prepare aligned nanofiber wound dressings (ANFDs). We then investigated the morphological, physicochemical, mechanical, and biological properties of fabricated ANDFs. The presence of ε-PL confers bactericidal properties while promoting epithelial and fibroblast adhesion, proliferation, and migration, confirming its cell selectivity. The clinical importance of the ANFDs was then demonstrated in a mice model of full-thickness diabetic wounds. The results confirm that ANFD treatment resulted in a higher rate of wound closure in the linear range of wound closure than wounds treated with silver dressings. Taken together, these results suggest the potential of antimicrobial ANFDs for the treatment of diabetic wounds.

In Eastern Indo-Gangetic Plains, traditional rice-wheat cropping system, reliant on wet puddling for rice and conventional tillage for wheat, has led to the soil degradation and stagnant yields, highlighting the need for sustainable practices. A field experiment (2018–2021) evaluated the impacts of tillage and residue management on productivity, energy efficiency, and soil health across three cropping systems: rice-wheat (RW), rice-maize (RM), and rice-chickpea (RC). Three tillage practices-conventional tillage (CT), reduced tillage (RT), and reduced tillage with 30 % residue retention (RTR30) were arranged in a randomized block design. The results showed that partial conservation agriculture (pCA) practices significantly enhanced the system productivity, with RM under RT achieving the highest yield (12.2 Mg ha−1). Reduced tillage consistently the minimized energy use, while pulses-based systems had the highest energy ratio (3.97). On average, total earthworm counts were 0.92 and 6.5 times higher during the rainy and post-rainy seasons in pCA treatments in comparison to CT. Earthworm species diversity was more in pCA-based production systems (RT/RTR30) than in CT. In rice-wheat cropping system, maximum bacterial and actinomycetes counts were observed in CT/FP followed by pCA production system. Maize-based systems under CT had the highest global warming potential, while the pulse-based systems had the lowest. The RM cropping system under RTR30 demonstrated the greatest increase in the soil organic carbon. In conclusion, adopting pCA management practices and incorporating the pulses can improve crop productivity, soil health, and sustainability, offering a promising path for enhancing food and nutritional security in rice-based production systems.

Intense cultivation with narrow row spacing in wheat, a common practice in the Indo-Gangetic plains of South Asia, renders the crop more susceptible to lodging during physiological maturity. This susceptibility, compounded by the use of traditional crop cultivars, has led to a substantial decline in overall crop productivity. In response to these challenges, a two-year field study on the system of wheat intensification (SWI) was conducted. The study involved three different cultivation methods in horizontal plots and four wheat genotypes in vertical plots, organized in a strip plot design. Our results exhibited that adoption of SWI at 20 cm × 20 cm resulted in significantly higher intercellular CO2 concentration (5.9-6.3%), transpiration rate (13.2-15.8%), stomatal conductance (55-59%), net photosynthetic rate (126-160%), and photosynthetically active radiation (PAR) interception (1.6-25.2%) over the existing conventional method (plant geometry 22.5 cm × continuous plant to plant spacing) of wheat cultivation. The lodging resistance capacity of both the lower and upper 3rd nodes was significantly higher in the SWI compared to other cultivation methods. Among different genotypes, HD 2967 demonstrated the highest recorded value for lodging resistance capacity, followed by HD 2851, HD 3086, and HD 2894. In addition, adoption of the SWI at 20 cm × 20 cm enhanced crop grain yield by 36.9-41.6%, and biological yield by 27.5-29.8%. Significantly higher soil dehydrogenase activity (12.06 μg TPF g-1 soil hr-1), arylsulfatase activity (82.8 μg p-nitro phenol g-1 soil hr-1), alkaline phosphatase activity (3.11 n moles ethylene g-1 soil hr-1), total polysaccharides, soil microbial biomass carbon, and soil chlorophyll content were also noted under SWI over conventional method of the production. Further, increased root volumes, surface root density and higher NPK uptake were recorded under SWI at 20×20 cm in comparison to rest of the treatments. Among the tested wheat genotypes, HD-2967 and HD-3086 had demonstrated notable increases in grain and biological yields, as well as improvements in the photosynthetically active radiation (PAR) and chlorophyll content. Therefore, adoption of SWI at 20 cm ×20 cm (square planting) with cultivars HD 2967 might be the best strategy for enhancing crop productivity and resource-use efficiency under the similar wheat growing conditions of India and similar agro-ecotypes of the globe.

Introduction:Cannabis sativa is utilized mainly for palliative care worldwide. Ovarian cancer (OC) is a lethal gynecologic cancer. A particular cannabis extract fraction ('F7′) and the Poly(ADP-Ribose) Polymerase 1 (PARP1) inhibitor niraparib act synergistically to promote OC cell apoptosis. Here we identified genetic pathways that are altered by the synergistic treatment in OC cell lines Caov3 and OVCAR3.Materials and methods: Gene expression profiles were determined by RNA sequencing and quantitative PCR. Microscopy was used to determine actin arrangement, a scratch assay to determine cell migration and flow cytometry to determine apoptosis, cell cycle and aldehyde dehydrogenase (ALDH) activity. Western blotting was used to determine protein levels.Results: Gene expression results suggested variations in gene expression between the two cell lines examined. Multiple genetic pathways, including Hippo/Wnt, TGF-β/Activin and MAPK were enriched with genes differentially expressed by niraparib and/or F7 treatments in both cell lines. Niraparib + F7 treatment led to cell cycle arrest and endoplasmic reticulum (ER) stress, inhibited cell migration, reduced the % of ALDH positive cells in the population and enhanced PARP1 cleavage.Conclusion: The synergistic effect of the niraparib + F7 may result from the treatment affecting multiple genetic pathways involving cell death and reducing mesenchymal characteristics.

Abstract In recent times Gallbladder cancer (GBC) incidences increased many folds in India and are being reported from arsenic hotspots identified in Bihar. The study aims to establish association between arsenic exposure and gallbladder carcinogenesis. In the present study, n = 200 were control volunteers and n = 152 confirmed gallbladder cancer cases. The studied GBC patient’s biological samples-gallbladder tissue, gallbladder stone, bile, blood and hair samples were collected for arsenic estimation. Moreover, n = 512 gallbladder cancer patients blood samples were also evaluated for the presence of arsenic to understand exposure level in the population. A significantly high arsenic concentration (p

The activity of daily living (ADL) related to stair climbing is one of the most compromised ADLs among the elders. It requires good muscle strength, balance, and range of motion (ROM) in the lower limb. We aimed to investigate the effects of Yoga complemented with physiotherapy (Intervention group) compared to only physiotherapy (control group) on lower limb muscle strength, balance, and ROMs among elders with ADL limitation in stair climbing. Sixty-five community-dwelling elders with compromised stair climbing were enrolled. Elders who consented to yoga along with physiotherapy were enrolled in the intervention arm. Data were collected and analyzed following 12 weeks of intervention. Endline assessment showed that the intervention group had more improvement in all test scores and ROMs as compared to the control group, although there were improvements in both arms as compared to baseline. Considering the beneficial effects of both interventions, yoga can be added to gain additional advantages. Trial Registration: Indian Clinical Trials Registry number CTRI/2021/08/035825.

Abstract Background and objectives Smart technology in nursing home settings has the potential to elevate an operation that manages more significant number of older residents. However, the concepts, definitions, and types of smart technology, integrated medical services, and stakeholders’ acceptability of smart nursing homes are less clear. This scoping review aims to define a smart nursing home and examine the qualitative evidence on technological feasibility, integration of medical services, and acceptability of the stakeholders. Methods Comprehensive searches were conducted on stakeholders’ websites (Phase 1) and 11 electronic databases (Phase 2), for existing concepts of smart nursing home, on what and how technologies and medical services were implemented in nursing home settings, and acceptability assessment by the stakeholders. The publication year was inclusive from January 1999 to September 2021. The language was limited to English and Chinese. Included articles must report nursing home settings related to older adults ≥ 60 years old with or without medical demands but not bed-bound. Technology Readiness Levels were used to measure the readiness of new technologies and system designs. The analysis was guided by the Framework Method and the smart technology adoption behaviours of elder consumers theoretical model. The results were reported according to the PRISMA-ScR. Results A total of 177 literature (13 website documents and 164 journal articles) were selected. Smart nursing homes are technology-assisted nursing homes that allow the life enjoyment of their residents. They used IoT, computing technologies, cloud computing, big data and AI, information management systems, and digital health to integrate medical services in monitoring abnormal events, assisting daily living, conducting teleconsultation, managing health information, and improving the interaction between providers and residents. Fifty-five percent of the new technologies were ready for use in nursing homes (levels 6–7), and the remaining were proven the technical feasibility (levels 1–5). Healthcare professionals with higher education, better tech-savviness, fewer years at work, and older adults with more severe illnesses were more acceptable to smart technologies. Conclusions Smart nursing homes with integrated medical services have great potential to improve the quality of care and ensure older residents’ quality of life.

Abstract Background The impact of misinformation about vapes’ relative harms compared with smoking may lead to increased tobacco-related burden of disease. To date, no systematic efforts have been made to chart interventions that mitigate vaping-related misinformation. We plan to conduct a scoping review that seeks to fill gaps in the current knowledge of interventions that mitigate vaping-related misinformation. Methods A scoping review focusing on interventions that mitigate vaping-related misinformation will be conducted. We will search (no date restrictions) MEDLINE, Scopus, EMBASE, CINAHL, PsycINFO, Web of Science Core Collection, Global Health, ERIC, and Sociological Abstracts. Gray literature will be identified using Disaster Lit, Google Scholar, Open Science Framework, governmental websites, and preprint servers (e.g., EuropePMC, PsyArXiv, MedRxiv, JMIR Preprints). Study selection will conform to Joanna Briggs Institute Reviewers’ Manual 2020 Methodology for JBI Scoping Reviews. Only English language, original studies will be considered for inclusion. Two reviewers will independently screen all citations, full-text articles, and abstract data. A narrative summary of findings will be conducted. Data analysis will involve quantitative (e.g., frequencies) and qualitative (e.g., content and thematic analysis) methods. Where possible, a single effect size of exposure to the mitigation of vaping-related misinformation will be calculated per sample. Similarly, where possible, each study will be coded for moderating characteristics to find and account for systematic differences in the size of the effect or outcome that is being analyzed. Quality will be appraised with the study quality assessment tools utilized by the National Heart, Lung, and Blood Institute. Findings will be subjected to several different publication bias tests: Egger’s regression test, Begg and Mazumdar’s ran correlation test, and generation of a funnel plot with effect sizes plotted against a corresponding standard error. Discussion Original research is urgently needed to design interventions to mitigate vaping-related misinformation. The planned scoping review will help to address this gap. Systematic review registration Open Science Framework osf/io/hy3tk.

Background: Effective communication that integrates the value of patient-centered care is important in healthcare encounters. Communication skills training (CST) has been indicated as effective in improving patient-centered communication behaviors. However, there is a paucity of studies on the impact of CST among Malaysian hospital pharmacists. Objective: This study aimed to evaluate the effects of a patient-centered CST program on patient-centered communication scores, communication self-efficacy, and attitudes toward concordance among pharmacists in public hospitals. Methods: A communication skills training (CST) program was conducted among hospital pharmacists. This training intervention was developed based on patient-centered communication frameworks and techniques, namely the Four Habits Model and motivational interviewing. A pre-test/post-test quasi-experimental design was implemented for the evaluation. Pharmacists underwent pre-test/post-test audiotaped simulated consultations and completed questionnaires, including the Revised United States–Leeds Attitudes Toward Concordance scale (RUS-LATCon) and Communication Self-Efficacy scale. The Four Habits Coding Scheme (FHCS) was used to evaluate patient-centered communication scores from the audiotapes, and the Wilcoxon signed-rank test was used to analyze for differences in the pre- and post-intervention scores. Results: A total of 38 pharmacists from four tertiary hospitals participated in this study and completed the pre-test. However, due to the impact of COVID-19, only 23 pharmacists completed the post-test data collection. Improvements were noted in the FHCS scores post-training, including items related to exploring patients' concerns, acceptability, and barriers to treatment. Based on the questionnaire, there was an improvement in recognizing patients' needs and potential medication uncertainty and an increase in the overall communication self-efficacy scores after the training. Conclusions: CST may help improve the adoption of patient-centered communication in pharmacists' consultations with patients.

ObjectiveThis study investigates whether volumes of intake in the first 24 h of life (24 HOL), in relation to birth weight (BW) and gestational age (GA), impact neonatal feeding intolerance (FI).MethodsThis study employed a retrospective chart review of 6,650 infants born at ≥35 weeks. The volumes of each formula feed per kg BW in the first 24 HOL were assessed. FI was defined as evidenced by chart documentation of emesis, abdominal distension, abdominal x-ray, and/or switching to a sensitive formula.ResultsOverall, the maximum volume of formula intake per feed was inversely correlated with GA and was higher in infants with FI (β = −1.39, p 8 ml/kg [adjusted odds ratio (AOR) = 2.5, 95% confidence interval (CI): 1.4–4.6] and formula switching in the exclusively formula-fed group with volumes >10.5 ml/kg [AOR = 2.2, 95% CI (1.8–2.6)] were high. In the breastfeeding group, the odds of FI increased by 2.8-, 4.6-, and 5.2-fold with 5–10, 10–15, and >15 ml/kg of supplementations, respectively.ConclusionA higher volume of intake in relation to BW often exceeds the physiological stomach capacity of newborns and is associated with early FI. Optimizing early feeding volumes based on infant BW and GA may decrease FI, which may be an issue of volume intolerance.

IntroductionWe investigated the effects of hormonal and non-hormonal oral contraceptives (OCs) on bone mass, mineralization, composition, mechanical properties, and metabolites in pubertal female SD rats.MethodsOCs were given for 3-, and 7 months at human equivalent doses. The combined hormonal contraceptive (CHC) was ethinyl estradiol and progestin, whereas the non-hormonal contraceptive (NHC) was ormeloxifene. MicroCT was used to assess bone microarchitecture and BMD. Bone formation and mineralization were assessed by static and dynamic histomorphometry. The 3-point bending test, nanoindentation, FTIR, and cyclic reference point indentation (cRPI) measured the changes in bone strength and material composition. Bone and serum metabolomes were studied to identify potential biomarkers of drug efficacy and safety and gain insight into the underlying mechanisms of action of the OCs.ResultsNHC increased bone mass in the femur metaphysis after 3 months, but the gain was lost after 7 months. After 7 months, both OCs decreased bone mass and deteriorated trabecular microarchitecture in the femur metaphysis and lumbar spine. Also, both OCs decreased the mineral: matrix ratio and increased the unmineralized matrix after 7 months. After 3 months, the OCs increased carbonate: phosphate and carbonate: amide I ratios, indicating a disordered hydroxyapatite crystal structure susceptible to resorption, but these changes mostly reversed after 7 months, indicating that the early changes contributed to demineralization at the later time. In the femur 3-point bending test, CHC reduced energy storage, resilience, and ultimate stress, indicating increased susceptibility to micro-damage and fracture, while NHC only decreased energy storage. In the cyclic loading test, both OCs decreased creep indentation distance, but CHC increased the average unloading slope, implying decreased microdamage risk and improved deformation resistance by the OCs. Thus, reduced bone mineralization by the OCs appears to affect bone mechanical properties under static loading, but not its cyclic loading ability. When compared to an age-matched control, after 7 months, CHC affected 24 metabolic pathways in bone and 9 in serum, whereas NHC altered 17 in bone and none in serum. 6 metabolites were common between the serum and bone of CHC rats, suggesting their potential as biomarkers of bone health in women taking CHC.ConclusionBoth OCs have adverse effects on various skeletal parameters, with CHC having a greater negative impact on bone strength.

The thermodynamical study of a mathematical model of unsteady natural convective MHD oscillatory flow through a porous medium-filled channel of infinite plates with chemical reaction and thermal radiation effect is taken into account in this respective research. The time-dependent flow governing equations (PDEs); comprises momentum, energy and concentration equations are derived for the concerned physical model and converted into dimensionless second-order ordinary differential equations (ODEs) for fluctuation of small amplitude. Further, the set of ODEs is solved by MATLAB's built-in dsolve function. The graphical analysis of fluid velocity, temperature and concentration profiles has been explicated for flow parameters. According to the results, the transient velocity and concentration profiles expand when the chemical reaction parameter and Schmidt number increase, whereas a strong magnetic field retards the transient velocity profile. In addition, the velocity, temperature, and concentration profiles decline whenever the frequency of oscillation is increased. The elucidated flow model and mathematical results are significant to be used in real-life applications, including packed bed reactors, chemical reactors, cooling the towers and rocket engines, designing of heat exchangers, sewage and wastewater treatment, chemical and magnetic filtration, and separation etc.

Summary: Sodium sulfate decahydrate (Na2SO4.10H2O, SSD), a low-cost phase change material (PCM), can store thermal energy. However, phase separation and unstable energy storage capacity (ESC) limit its use. To address these concerns, eight polymer additives—sodium polyacrylate (SPA), carboxymethyl cellulose (CMC), Fumed silica (SiO2), potassium polyacrylate (PPA), cellulose nanofiber (CNF), hydroxyethyl cellulose (HEC), dextran sulfate sodium (DSS), and poly(sodium 4-styrenesulfonate) (PSS)—were used to explore several stabilization mechanisms. The ESC of PCMs deteriorated when thickeners, SPA, PPA, and CNF, were added. DSS-modified PCMs exhibited greater stability up to 150 cycles. Rheology measurements indicated that DSS did not impact SSD viscosity significantly during stabilization. Dynamic light scattering showed that DSS reduces SSD particle size and electrostatically suspends salt particles in a stable homogeneous solution, avoiding phase separation. This study proposes a promising method to improve the thermal stability of salt hydrate PCMs by utilizing polyelectrolyte-salt hydrate mixture for thermal energy storage applications.

Abstract Given the spasmodic increment in antimicrobial resistance (AMR), world is on the verge of “post-antibiotic era”. It is anticipated that current SARS-CoV2 pandemic would worsen the situation in future, mainly due to the lack of new/next generation of antimicrobials. In this context, nanoscale materials with antimicrobial potential have a great promise to treat deadly pathogens. These functional materials are uniquely positioned to effectively interfere with the bacterial systems and augment biofilm penetration. Most importantly, the core substance, surface chemistry, shape, and size of nanomaterials define their efficacy while avoiding the development of AMR. Here, we review the mechanisms of AMR and emerging applications of nanoscale functional materials as an excellent substitute for conventional antibiotics. We discuss the potential, promises, challenges and prospects of nanobiotics to combat AMR. Graphical Abstract

Type 2 diabetes (T2D) affects the functional behavior of vertebra bone by altering its structural and mechanical properties. The vertebral bones are responsible to carry the body weight and it remains under prolonged constant load which results to viscoelastic deformation. The effect of T2D on the viscoelastic behavior of vertebral bone is not well explored yet. In this study, the effects of T2D on the creep and stress relaxation behavior of vertebral bone are investigated. Also, this study established a correlation between T2D associated alteration in macromolecular structure and viscoelastic behavior of vertebra. In this study T2D female rat SD model was used. The obtained results demonstrated a significant reduction in the amount of creep strain (p ≤ 0.05) and stress relaxation (p ≤ 0.01) in T2D specimens than the control. Also, the creep rate was found significantly lower in T2D specimens. On the other hand, molecular structural parameters such as mineral-to-matrix ratio (control vs T2D: 2.93 ± 0.78 vs 3.72 ± 0.53; p = 0.02), and non-enzymatic cross link ratio (NE-xL) (control vs T2D: 1.53 ± 0.07 vs 3.84 ± 0.20; p = 0.01) were found significantly altered in T2D specimens. Pearson linear correlation tests show a significant correlation; between creep rate and NE-xL (r = −0.94, p

Abstract Background The duration and impact of the COVID-19 pandemic depends in a large part on individual and societal actions which is influenced by the quality and salience of the information to which they are exposed. Unfortunately, COVID-19 misinformation has proliferated. To date, no systematic efforts have been made to evaluate interventions that mitigate COVID-19-related misinformation. We plan to conduct a scoping review that seeks to fill several of the gaps in the current knowledge of interventions that mitigate COVID-19-related misinformation. Methods A scoping review focusing on interventions that mitigate COVID-19 misinformation will be conducted. We will search (from January 2020 onwards) MEDLINE, EMBASE, CINAHL, PsycINFO, Web of Science Core Collection, Africa-Wide Information, Global Health, WHO Global Literature on Coronavirus Disease Database, WHO Global Index Medicus, and Sociological Abstracts. Gray literature will be identified using Disaster Lit, Google Scholar, Open Science Framework, governmental websites, and preprint servers (e.g., EuropePMC, PsyArXiv, MedRxiv, JMIR Preprints). Study selection will conform to Joanna Briggs Institute Reviewers’ Manual 2020 Methodology for JBI Scoping Reviews. Only English language, original studies will be considered for inclusion. Two reviewers will independently screen all citations, full-text articles, and abstract data. A narrative summary of findings will be conducted. Data analysis will involve quantitative (e.g., frequencies) and qualitative (e.g., content and thematic analysis) methods. Discussion Original research is urgently needed to design interventions to mitigate COVID-19 misinformation. The planned scoping review will help to address this gap. Systematic review registrations Systematic Review Registration: Open Science Framework (osf/io/etw9d).

Abstract Background Despite the growing morbidity and mortality rates associated with opioid use disorder, a large gap still exists between treatment need and capacity. Low-threshold clinics utilizing medication for opioid use disorder (MOUD) treatment can increase treatment access but are understudied, and little is known about how patient demographic characteristics are associated with their social support and functioning in these settings. Methods We used multivariate regression to estimate associations between demographic characteristics and self-reported social support or functioning indicators among patients receiving MOUD in a low-threshold clinic using several validated instruments administered at intake: Behavior and Symptom Identification Scale, Brief Pain Inventory, and Life Events Checklist for DSM-5. Patients initiating MOUD treatment between April 1 and December 31, 2017, with complete surveys were included (N=582). Results Patients were primarily male (62%), aged 34 or older (53%), non-Hispanic White (79%), separated or not married (86%), and unemployed (64%). Over 20% did not live in a house or apartment in the past month. Women were more likely to “get along” with people outside their family or in social situations and to identify their partner as their source of support. Women, non-White, and older patients were at higher risk of social functioning-disrupting events (physical/sexual assaults or experiencing chronic pain), while employment and housing were protective against exposure to these trauma-related events. However, employment and housing also decreased the odds of talking with others about substance use. The aforementioned results were obtained from multivariate logistic regression models and were significant to p

Immune surveillance and adaptive immune responses, involving continuously circulating and tissue-resident T-lymphocytes, provide host defense against infectious agents and possible malignant transformation while avoiding autoimmune tissue damage. Activation, migration, and deployment of T-cells to affected tissue sites are crucial for mounting an adaptive immune response. An effective adaptive immune defense depends on the ability of T-cells to dynamically reprogram their metabolic requirements in response to environmental cues. Inability of the T-cells to adapt to specific metabolic demands may skew cells to become either hyporesponsive (creating immunocompromised conditions) or hyperactive (causing autoimmune tissue destruction). Here, we review maladaptive T-cell metabolic fitness that can cause autoimmune diseases and discuss how T-cell metabolic programs can potentially be modulated to achieve therapeutic benefits.

Abstract Objective News coverage around vaping-related events may have furthered misconceptions regarding the relative harms of vapes. Such information may influence the decisions of individuals who smoke, around switching to vaping, potentially affecting the overall tobacco mortality burden. Thus, it is prudent to study how news events (e.g., 2019 vaping illness epidemic) are associated with vape sales in the United States, to possibly reduce the tobacco mortality burden. Methods We used weekly retail sales data for e-cigarettes (30 December 2018 - 28 December 2019) from the US retail scanner data compiled by the Nielsen Company. We used an interrupted time series design with segmented regression analysis to determine immediate and longer-term impacts of individual news events (e.g. Trump administration’s planned ban on some flavored vaping products) on vape sales, controlling for pre-existing trends. Results Unexpectedly, we noted a statistically significant positive relationship between vape sales and the CDC announcing an investigation into vaping-related illnesses (Change: 6.59%, Estimate: 0.066; 95% CI: 0.036, 0.092; P

Abstract Background Open online forums like Reddit provide an opportunity to quantitatively examine COVID-19 vaccine perceptions early in the vaccine timeline. We examine COVID-19 misinformation on Reddit following vaccine scientific announcements, in the initial phases of the vaccine timeline. Methods We collected all posts on Reddit (reddit.com) from January 1 2020 - December 14 2020 (n=266,840) that contained both COVID-19 and vaccine-related keywords. We used topic modeling to understand changes in word prevalence within topics after the release of vaccine trial data. Social network analysis was also conducted to determine the relationship between Reddit communities (subreddits) that shared COVID-19 vaccine posts, and the movement of posts between subreddits. Results There was an association between a Pfizer press release reporting 90% efficacy and increased discussion on vaccine misinformation. We observed an association between Johnson and Johnson temporarily halting its vaccine trials and reduced misinformation. We found that information skeptical of vaccination was first posted in a subreddit (r/Coronavirus) which favored accurate information and then reposted in subreddits associated with antivaccine beliefs and conspiracy theories (e.g. conspiracy, NoNewNormal). Conclusions Our findings can inform the development of interventions where individuals determine the accuracy of vaccine information, and communications campaigns to improve COVID-19 vaccine perceptions, early in the vaccine timeline. Such efforts can increase individual- and population-level awareness of accurate and scientifically sound information regarding vaccines and thereby improve attitudes about vaccines, especially in the early phases of vaccine roll-out. Further research is needed to understand how social media can contribute to COVID-19 vaccination services.

Missing data are a universal data quality problem in many domains, leading to misleading analysis and inaccurate decisions. Much research has been done to investigate the different mechanisms of missing data and the proper techniques in handling various data types. In the last decade, machine learning has been utilized to replace conventional methods to address the problem of missing values more efficiently. By studying and analyzing recently proposed methods using machine learning approaches, vital adoptions in accuracy, performance, and time consumed can be highlighted. This study aimed to help data analysts and researchers address the limitations of machine learning imputation methods by conducting a systematic literature review to provide a comprehensive overview of using such methods to impute missing values. Novel proposed machine learning approaches used for data imputation are analyzed and summarized to assist researchers in selecting a proper machine learning method based on several factors and settings. The review was performed on research studies published between 2016 and 2021 on adopting machine learning to impute missing values, focusing on their strengths and limitations. A total of 684 research articles from various scientific databases were analyzed using search engines, and 94 of them were selected as primary studies. Finally, several recommendations were given to guide future researchers in applying machine learning to impute missing values.

IntroductionIn obese humans, Coleus forskohlii root extract (CF) protects against weight gain owing to the presence of forskolin, an adenylate cyclase (AC) activator. As AC increases intracellular cyclic adenosine monophosphate (cAMP) levels in osteoblasts that has an osteogenic effect, we thus tested the skeletal effects of a standardized CF (CFE) in rats.MethodsConcentrations of forskolin and isoforskolin were measured in CFE by HPLC. CFE and forskolin (the most abundant compound present in CFE) were studied for their osteogenic efficacy in vitro by alkaline phosphatase (ALP), cAMP and cyclic guanosine monophosphate (cGMP) assays. Femur osteotomy model was used to determine the osteogenic dose of CFE. In growing rats, CFE was tested for its osteogenic effect in intact bone. In adult ovariectomized (OVX) rats, we assessed the effect of CFE on bone mass, strength and material. The effect of forskolin was assessed in vivo by measuring the expression of osteogenic genes in the calvarium of rat pups.ResultsForskolin content in CFE was 20.969%. CFE increased osteoblast differentiation and intracellular cAMP and cGMP levels in rat calvarial osteoblasts. At 25 mg/kg (half of human equivalent dose), CFE significantly enhanced calcein deposition at the osteotomy site. In growing rats, CFE promoted modeling-directed bone formation. In OVX rats, CFE maintained bone mass and microarchitecture to the level of sham-operated rats. Moreover, surface-referent bone formation in CFE treated rats was significantly increased over the OVX group and was comparable with the sham group. CFE also increased the pro-collagen type-I N-terminal propeptide: cross-linked C-telopeptide of type-I collagen (PINP : CTX-1) ratio over the OVX rats, and maintained it to the sham level. CFE treatment decreased the OVX-induced increases in the carbonate-to-phosphate, and carbonate-to-amide-I ratios. CFE also prevented the OVX-mediated decrease in mineral crystallinity. Nanoindentation parameters, including modulus and hardness, were decreased by OVX but CFE maintained these to the sham levels. Forskolin stimulated ALP, cAMP and cGMP in vitro and upregulated osteogenic genes in vivo.ConclusionCFE, likely due to the presence of forskolin displayed a bone-conserving effect via osteogenic and anti-resorptive mechanisms resulting in the maintenance of bone mass, microarchitecture, material, and strength.

Most studies reporting prevalence of obesity use actual weight and height measurements. Self-reported weight and height have been used in epidemiological studies as they have been shown to be reliable, convenient, and inexpensive alternatives to actual measurements. However, the accuracy of self-reported weight and height might vary in different regions because of the difference in health awareness and social influences. This study aims to determine the accuracy and reliability of self-reported weight and height compared to actual measured weight and height among adults in Malaysia. This was a cross-sectional study conducted at the community level during blood pressure screening campaigns. Participants self-reported their weight and height in a questionnaire survey. Their weight and height were validated using measurements by researchers on the same setting. Body mass index (BMI) was defined as underweight (

There are rising concerns among the medical community and the public regarding the side effects of different vaccines developed throughout the world and their short and long-term effects, particularly COVID19 vaccines. Most notably, post-vaccination demyelinating diseases such as acute disseminated encephalomyelitis, transverse myelitis, and multiple sclerosis relapses have been reported. We present a case of a 32-year-old male who presented with a 2 weeks history of acute confusional state and imbalance 1 week after receiving the second dose of COVID19 vaccination. MRI findings showed typical distribution of neuromyelitis optica spectrum disorder and the patient was positive for AQP4 IgG. The pathogenesis behind developing neuromyelitis optica and vaccines is still unknown. Few case reports have been reported of post-vaccination neuromyelitis optica spectrum disorder but to our knowledge, this would be the first case published of neuromyelitis optica following exposure to COVID19 vaccine.

Abstract Background There are limited studies on blocking and men who have sex with men (MSM) health outcomes. We need such data in China, to better understand the relationship between Chinese MSM gay app use and health outcomes, thus providing insight on risky sexual behaviors and HIV transmission among Chinese MSM - one of the world’s largest MSM communities. Blocking someone is when users select a function on an app to prevent another user from contacting them and being blocked is when someone is prevented from contacting another user. We studied the correlates of blocking on the world’s largest gay dating app among Chinese MSM (N = 208). Methods We conducted a cross-sectional survey as part of an HIV testing intervention in Guangzhou, China, May–December 2019. Using logistic regression models, we estimated the correlates of blocking (e.g. sociodemographic characteristics, sexual behavior, HIV testing history, social network data). Results MSM had a mean age of 27.9 years (SD = 7.1) and median of one sexual partner in the last 3 months. About 62% had blocked someone in their lifetime and 46% had been blocked in their lifetime. Each additional male partner was associated with an 87% (aOR = 1.87, 95%CI = 1.03, 3.40) increased chance of being blocked. Reporting a versatile sexual role was related with a 90% (aOR = 0.10, 95%CI = 0.02, 0.45) decreased likelihood of blocking behavior and an 86% (aOR = 0.14, 95%CI = 0.04, 0.46) reduced chance of being blocked. Conclusions Number of male partners may be associated with blocking behavior, with implications for the design of online sexual health interventions.

The auxin efflux transporter proteins called PINs ferry auxin from its source to sinks in particular directions depending on their polar localizations in the plasma membrane, thus facilitating the development of the entire plant architecture. The rice genome has 12 PIN genes distributed over eight chromosomes. To study their roles in plant development, abiotic stress responsiveness, and shaping an auxin-dependent root architecture, a genome-wide analysis was carried out. Based on phylogeny, cellular localization, and hydrophilic loop domain size, the PINs were categorized into canonical and noncanonical PINs. PINs were found expressed in all of the organs of plants that emphasized their indispensable role throughout the plant’s life cycle. We discovered that PIN5C and PIN9 were upregulated during salt and drought stress. We also found that regardless of its cellular level, auxin functioned as a molecular switch to turn on auxin biosynthesis genes. On the contrary, although PIN expression was upregulated upon initial treatment with auxin, prolonged auxin treatment not only led to their downregulation but also led to the development of auxin-dependent altered root formation in rice. Our study paves the way for developing stress-tolerant rice and plants with a desirable root architecture by genetic engineering.

The Kargil Formation in the region of Ladakh (northern India) is known for its late Oligocene mammal fauna of both large mammals and rodents. New excavations in the area yielded a maxillary fragment of an insectivore with three premolars and two roots of a canine. The fossil record of the insectivores on the Indian subcontinent is as yet scanty. Based on the peculiar morphology of the last premolar, the Ladakh fossil could be identified as belonging to a new species of Erinaceinae, Ladakhechinus iugummontis n. gen. n. sp. The new find confirms the large diversity among hedgehogs in Asia during the Oligocene.

Understanding the determinants of health is essential for designing effective strategies to advance economic growth, reduce disease and disability, and enhance quality of life. We undertake a comprehensive outlook on public health by incorporating three metrics — life expectancy (LE), healthy life expectancy (HLE), and the discrepancy between the two. We investigate the effects of various health and socio-economic factors on these metrics and employ causal machine learning and statistical methods such as propensity score matching, X-learners, and causal forests to calculate treatment effects. An increase in basic water services and public health expenditure significantly increased average LE whereas high human immunodeficiency virus (HIV) prevalence rates and poverty rates reduced average LE. High gross national income (GNI) per capita and moderate body mass index (BMI) increased HLE whilst high HIV prevalence rates decreased HLE. High public health expenditure and high GNI per capita expand the gap between HLE and LE whereas high HIV prevalence rates and moderate BMI diminish this gap. Results suggest that policymakers should utilize governmental resources to improve public health infrastructure rather than provide fiscal incentives to encourage private healthcare infrastructure. Additionally, more emphasis should be placed on increasing educational levels of the general public by increasing educational expenditure and making educational institutions, public and private, more accountable.