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4 results on '"Oil Crops Research Institute, Key Laboratory of Biology and Genetic Improvement of Oil Crops"'

1. PDAT regulates PE as transient carbon sink alternative to triacylglycerol in Nannochloropsis

Author

Juan Yang, Jin Liu, Yufang Pan, Eric Maréchal, Alberto Amato, Meijing Liu, Yangmin Gong, Yantao Li, Hanhua Hu, Key Laboratory of Algal Biology, College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Peking University [Beijing], LIPID, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Oil Crops Research Institute, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture-Chinese Academy of Agricultural Sciences (CAAS), Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science and University of Maryland Baltimore County, The National Natural Science Foundation of China (41976119 and 91751117), Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDPB18), A CEA-Chinese Academy of Sciences bilateral program, The National Key R&D Program of China (2018YFA0902500), ANR-10-LABX-0049,GRAL,Grenoble Alliance for Integrated Structural Cell Biology(2010), and ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017)

Subjects
Carbon Sequestration, Physiology, Phosphatidylethanolamines, Saccharomyces cerevisiae, Plant Science, Carbon Dioxide, Plants, Microalgae, Genetics, lipids (amino acids, peptides, and proteins), [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Diacylglycerol O-Acyltransferase, Acyltransferases, Triglycerides, Research Articles
Abstract

Triacylglycerols (TAGs) are the main storage lipids in photosynthetic organisms under stress. In the oleaginous alga Nannochloropsis oceanica, while multiple acyl CoA:diacylglycerol (DAG) acyltransferases (NoDGATs) are involved in TAG production, the role of the unique phospholipid:DAG acyltransferase (NoPDAT) remains unknown. Here, we performed a functional complementation assay in TAG-deficient yeast (Saccharomyces cerevisiae) and an in vitro assay to probe the acyltransferase activity of NoPDAT. Subcellular localization, overexpression, and knockdown (KD) experiments were also conducted to elucidate the role of NoPDAT in N. oceanica. NoPDAT, residing at the outermost plastid membrane, does not phylogenetically fall into the clades of algae or plants and uses phosphatidylethanolamine (PE) and phosphatidylglycerol with 16:0, 16:1, and 18:1 at position sn-2 as acyl-donors in vivo. NoPDAT KD, not triggering any compensatory mechanism via DGATs, led to an ∼30% decrease of TAG content, accompanied by a vast accumulation of PEs rich in 16:0, 16:1, and 18:1 fatty acids (referred to as “LU-PE”) that was positively associated with CO2 availability. We conclude that the NoPDAT pathway is parallel to and independent of the NoDGAT pathway for oil production. LU-PE can serve as an alternative carbon sink for photosynthetically assimilated carbon in N. oceanica when PDAT-mediated TAG biosynthesis is compromised or under stress in the presence of high CO2 levels.

Published
2022
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2. A Two-step Strategy for High-Value-Added Utilization of Rapeseed Meal by Concurrent Improvement of Phenolic Extraction and Protein Conversion for Microbial Iturin A Production

Author

Meng Wang, Chen Yang, Jean Marie François, Xia Wan, Qianchun Deng, Danyang Feng, Shiyu Deng, Shouwen Chen, Fenghong Huang, Wenchao Chen, Yangmin Gong, Chinese Academy of Agricultural Sciences (CAAS), Oil Crops Research Institute, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture-Chinese Academy of Agricultural Sciences (CAAS), Oil Crops and Lipids Process Technology National and Local Joint Engineering Laboratory, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Hubei University, National Natural Science Foundation of China (NSFC)32001620, Natural Science Foundation of Hubei Province2019CFB378, Youth talent development program of Hubei province, Earmarked Fund for China Agriculture Research SystemCARS-12, and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
0106 biological sciences, Histology, Antioxidant, Rapeseed, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Bacillus amyloliquefaciens, medicine.medical_treatment, Biomedical Engineering, Bioengineering, phenolic compounds, Bacterial growth, Raw material, protein residue, 01 natural sciences, 03 medical and health sciences, medicine, Food science, Original Research, 030304 developmental biology, 2. Zero hunger, 0303 health sciences, Residue (complex analysis), biology, Chemistry, Extraction (chemistry), Bioengineering and Biotechnology, biology.organism_classification, fungal pretreatment, two-step strategy, rapeseed meal, Fermentation, microbial iturin A production, TP248.13-248.65, 010606 plant biology & botany, Biotechnology
Abstract

Rapeseed meal (RSM) is a major by-product of oil extraction from rapeseed, consists mainly of proteins and phenolic compounds. The use of RSM as protein feedstock for microbial fermentation is always hampered by phenolic compounds, which have antioxidant property with health-promoting benefits but inhibit bacterial growth. However, there is still not any good process that simultaneously improve extraction efficiency of phenolic compounds with conversion efficiency of protein residue into microbial production. Here we established a two-step strategy including fungal pretreatment followed by extraction of phenolic compounds. This could not only increase extraction efficiency and antioxidant property of phenolic compounds by about 2-fold, but also improve conversion efficiency of protein residue into iturin A production by Bacillus amyloliquefaciens CX-20 by about 33%. The antioxidant and antibacterial activities of phenolic extracts were influenced by both total phenolic content and profile, while microbial feedstock value of residue was greatly improved because protein content was increased by ∼5% and phenolic content was decreased by ∼60%. Moreover, this two-step process resulted in isolating more proteins from RSM, bringing iturin A production to 1.95 g/L. In conclusion, high-value-added and graded utilization of phenolic extract and protein residue from RSM with zero waste is realized by a two-step strategy, which combines both benefits of fungal pretreatment and phenolic extraction procedures.

Published
2021
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3. The genome of the mesopolyploid crop species Brassica rapa

Author

Binghang Liu, Wei Hua, Zhenyu Li, Chunfang Peng, Jeong-Sun Kim, J. Chris Pires, Beom-Seok Park, Shengyi Liu, Shu Zhang, Ye Yin, Chaobo Tong, Tae-Ho Lee, Yang Qiu, Di Shen, Sanwen Huang, Qingle Cai, Jin A Kim, Shunmou Huang, Lu Fang, Graham J.W. King, Nizar Drou, Bo Li, Gavin C. Conant, Bernd Weisshaar, Jiaohui Xu, Jun Wang, Zhiyong Xiong, Fei Li, Chi Song, Zhonghua Zhang, Matthew G. Links, Rifei Sun, Jinpeng Wang, Fiona Fraser, Chuyu Lin, Yongcui Liao, Chushin Koh, Wei Fan, Su-Ryun Choi, Shusei Sato, Shifeng Cheng, Xiang Zhao, Jeong-Hwan Mun, Hideki Hirakawa, Harry Belcram, Zhiwen Wang, Martin Trick, Alice Hayward, Xixiang Li, Shujiang Zhang, Desheng Mu, Satoshi Tabata, Silong Sun, Isobel A. P. Parkin, Bo Liu, Xu Tan, Nirala Ramchiary, Guusje Bonnema, Yinqi Bai, Yoshihiro Narusaka, Yongchen Du, Hui Wang, Julie Poulain, Xun Xu, Yupeng Wang, Yan Wang, Haibao Tang, Zhenyi Wang, Meixia Zhao, David Edwards, Jinling Meng, Chris Duran, Andrew H. Paterson, Soo-Jin Kwon, Hanzhong Wang, Feng Cheng, Heling Zhou, Geng Chunyu, Jacqueline Batley, Bo Wang, Yingrui Li, Junyi Wang, Gilles Lassalle, Boulos Chalhoub, Jianwen Li, Yong Pyo Lim, Quanfei Huang, Eleni Soumpourou, Jian Wu, Katsunori Hatakeyama, Xiaowu Wang, Paul J. Berkman, Ian Bancroft, Jérémy Just, Jingyin Yu, Hui Guo, Kui Wu, Li Wang, Ruiqiang Li, Xiyin Wang, Huizhe Jin, Haiping Wang, Andrew G. Sharpe, Michael Freeling, Hiroshi Abe, Mina Jin, Jie Deng, Jiumeng Min, Jingping Li, Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS), Zhejiang University, Oil Crops Research Institute, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture-Chinese Academy of Agricultural Sciences (CAAS), Department of Plant Pathology, University of Kentucky (UK), Rural Development Administration, Department of Biology, University of York [York, UK], Hasso Plattner Institute (HPI), University of Potsdam = Universität Potsdam, Institute of Vegetable sand Flowers, Key Laboratory for Ultrafine Materials, East China University of Science and Technology, Beijing Genom Inst Shenzhen, University of Georgia [USA], School of Life Sciences, Center of Genomics and Computational Biology, Hebei United University, University of California [Berkeley] (UC Berkeley), University of California (UC), The Hospital for sick children [Toronto] (SickKids), University of Toronto, Unité de recherche en génomique végétale (URGV), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), State Key Laboratory for Mineral Deposits Research, Nanjing University (NJU), Amélioration des Plantes et Biotechnologies Végétales (APBV), and Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST

Subjects
Genome evolution, Chromosomes, Artificial, Bacterial, lineages, diversification, [SDV]Life Sciences [q-bio], Molecular Sequence Data, Arabidopsis, Biology, Genes, Plant, Genome, Chromosomes, Plant, Evolution, Molecular, Polyploidy, Contig Mapping, Laboratorium voor Plantenveredeling, Gene density, Gene Duplication, Brassica rapa, evolution, genetics, Gene, Phylogeny, Genetics, Whole genome sequencing, repressor, EPS-3, fungi, duplicated genes, food and beverages, Molecular Sequence Annotation, alignment, Genome project, Sequence Analysis, DNA, sequence, Plant Breeding, Paleopolyploidy, plant arabidopsis-thaliana, Genome, Plant, triplication
Abstract

The Brassicaceae family which includes Arabidopsis thaliana, is a natural priority for reaching beyond botanical models to more deeply sample angiosperm genomic and functional diversity. Here we report the draft genome sequence and its annoation of Brassica rapa, one of the two ancestral species of oilseed rape. We modeled 41,174 protein-coding genes in the B. rapa genome. B. rapa has experienced only the second genome triplication reported to date, with its close relationship to A. thaliana providing a useful outgroup for investigating many consequences of triplication for its structural and functional evolution. The extent of gene loss (fractionation) among triplicated genome segments varies, with one copy containing a greater proportion of genes expected to have been present in its ancestor (70 percent) than the remaining two (46 percent and 36 percent). Both a generally rapid evolutionary rate, and specific copy number amplifications of particular gene families, may contribute to the remarkable propensity of Brassica species for the development of new morphological variants. The B. rapa genome provides a new resource for comparative and evolutionary analysis of the Brassicaceae genomes and also a platform for genetic improvement of Brassica oil and vegetable crops.

Published
2011
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4. Genome-wide analysis and expression patterns of lipid phospholipid phospholipase gene family in Brassica napus L.

Author

Su W, Raza A, Zeng L, Gao A, Lv Y, Ding X, Cheng Y, and Zou X

Subjects
Gene Expression Regulation, Plant, Multigene Family, Phospholipases, Phospholipids, Phylogeny, Plant Proteins genetics, Plant Proteins metabolism, Stress, Physiological genetics, Brassica napus genetics, Brassica napus metabolism
Abstract

Background: Lipid phosphate phosphatases (LPP) are critical for regulating the production and degradation of phosphatidic acid (PA), an essential signaling molecule under stress conditions. Thus far, the LPP family genes have not been reported in rapeseed (Brassica napus L.)., Results: In this study, a genome-wide analysis was carried out to identify LPP family genes in rapeseed that respond to different stress conditions. Eleven BnLPPs genes were identified in the rapeseed genome. Based on phylogenetic and synteny analysis, BnLPPs were classified into four groups (Group I-Group IV). Gene structure and conserved motif analysis showed that similar intron/exon and motifs patterns occur in the same group. By evaluating cis-elements in the promoters, we recognized six hormone- and seven stress-responsive elements. Further, six putative miRNAs were identified targeting three BnLPP genes. Gene ontology analysis disclosed that BnLPP genes were closely associated with phosphatase/hydrolase activity, membrane parts, phosphorus metabolic process, and dephosphorylation. The qRT-PCR based expression profiles of BnLPP genes varied in different tissues/organs. Likewise, several gene expression were significantly up-regulated under NaCl, PEG, cold, ABA, GA, IAA, and KT treatments., Conclusions: This is the first report to describe the comprehensive genome-wide analysis of the rapeseed LPP gene family. We identified different phytohormones and abiotic stress-associated genes that could help in enlightening the plant tolerance against phytohormones and abiotic stresses. The findings unlocked new gaps for the functional verification of the BnLPP gene family during stresses, leading to rapeseed improvement., (© 2021. The Author(s).)

Published
2021
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