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Hydrogen, as a kind of green and efficient energy, plays an increasingly important role in current social development. Hydrogen storage technology is considered to be one of the main bottlenecks in limiting the large-scale application of hydrogen energy. The solid-state hydrogen storage technology based on Mg-based materials has received extensive attention due to its advantages of high hydrogen capacity, good reversibility, and low cost, but there are still shortcomings such as high reaction temperature, large energy consumption, and slow reaction kinetics. In order to solve these problems, this article proposes a new method of using microwave plasma to ionize hydrogen into H− ion. The possible activation mechanism of microwave plasma to improve the hydrogen storage properties is put forward. Based on the activation mechanism, the thermodynamic performance of Mg-based hydrogen storage is evaluated using density functional theory. It is concluded that the reaction temperature is significantly reduced from 339°C to 109°C with the help of microwave plasma. In addition, the comparison between the conventional heating hydrogen storage process based on MgH2 and microwave enhanced advanced hydrogen storage process based on MgH2 systems coupled with solid oxide fuel cells for heat and power generation is conducted to evaluate the economic feasibility. The results show that the energy consumption cost of the proposed microwave plasma enhancing hydrogen storage system is approximately 1.71 $/kgH2, which is about 50% of the energy consumption cost of the conventional system. [ABSTRACT FROM AUTHOR]

The impact of plasma temperature on the formation and evolution of distinct and unique molecular species recorded as molecular signatures from laser induced plasma (LIP) was examined in this study. A definitive correlation between plasma temperature and the formation of molecular species has been established through a comprehensive temporal analysis of BO and BO2 molecular bands. The utilization of the signal-to-envelope ratio diagrams allowed for pinpointing the optimal temperature range for molecule emission. Notably, the molecule BO exhibited an ideal temperature range of 10,000–11,000 K for the proper formation of molecular emission bands, while BO2 preferred temperatures between 8,000–9,000 K. These optimal temperature ranges remained consistent regardless of the laser irradiation wavelength or ambient gas conditions of Ar, air or He studied in this work. These key findings highlight the significant influence of plasma temperature in shaping the molecular species observed in LIP. [ABSTRACT FROM AUTHOR]

The theory of ion-acoustic solitons in nonrelativistic fully degenerate plasmas and nonrelativistic and ultra-relativistic degenerate plasmas at low temperatures is known. We consider a multi-component relativistic degenerate electron-positron-ion plasma at finite temperatures. Specifically, we focus on the intermediate region where the particle's thermal energy (k B T) and the rest mass energy (m c 2) do not differ significantly, i.e., k B T ∼ m c 2 . However, the Fermi energy (k B T F) is larger than the thermal energy and the normalized chemical energy ( ξ = μ / k B T ) is positive and finite. Two different parameter regimes with β ≡ k B T / m c 2 < 1 and β > 1 , relevant for astrophysical plasmas, are defined, and the existence of small amplitude ion-acoustic solitons in these regimes are studied, including the critical cases where the known KdV (Korteweg–de Vries) theory fails. We show that while the solitons with both the positive (compressive) and negative (rarefactive) potentials coexist in the case of β < 1 , only compressive solitons can exist in the other regime (β > 1) . Furthermore, while the rarefactive solitons within the parameter domains of β and ξ can evolve with increasing amplitude and hence increasing energy, the energy of compressive solitons reaches a steady state. [ABSTRACT FROM AUTHOR]

A possibility of studying effects of the main background ions formed by the main elements of inductively coupled plasma (H, N, O, and Ar) at the working parameters of the normal ("hot") plasma mode by thermodynamic modeling is assessed. Such ions, responsible for the strongest spectral interferences in the mass spectra are always observed upon the injection of aqueous ("wet") sample solutions into inductively coupled plasma mass spectrometers (ICP MS). The quantitative composition of the main background ions in an ICP MS is calculated as a function of plasma temperature in the temperature range from 3000 to 8000 K using thermodynamic modeling. The results of modeling were compared with the experimental data on the measured mass spectra of the main background ions and a high degree of correlation between the theoretical and experimental results was shown. The agreement between the results of calculations the experimental data validates the thermodynamic model of thermochemical processes in an ICP MS used and its applicability to subsequent calculations in fulfilling analytical tasks. A possibility of the unambiguous assessment of gas-kinetic plasma temperature is confirmed by comparing the theoretical and experimental mass spectra of the main ICP background ions in a normal mode. It was found that the calculated and experimental data on the concentration of only NO+ ions do not agree with the regularities noticed for the other background ions in the normal ICP mode. [ABSTRACT FROM AUTHOR]

In this investigation, a spectroscopic optical emission test was used to investigate the characteristics of gold plasmas that were produced in the surrounding environment using an Nd: YAG system at a wavelength of 1064 nm and had an energy output that varied from 400 to 600 mJ. The emission spectrum profiles of (Au) were analyzed and recorded using a spectrometer to aid in the extraction of vital plasma features (i.e., T e and n e ). Plasma temperature was determined using the Boltzmann plot method, and electron density was determined using the Stark broadening method. The temperature was between (0.91 and 0.94) eV, while the electron density ranged between (4.79 and 5.26) × 1 0 1 7 cm − 3 . The rest of the plasma parameters were calculated by the mathematical equations of the method used (Boltzmann Plott). [ABSTRACT FROM AUTHOR]

Flow ultrasound generators are devices that emit high-frequency sound waves due to the hydrodynamic instability of the supersonic flow. In the electric power industry, such generators are used in arc quenching systems of high-voltage gas blast circuit breakers. The design of the flow ultrasound generator includes a nozzle and a hollow cylindrical resonator. Self-oscillations of the sealing waves occur when a supersonic gas jet collides with a resonator. This article is devoted to the analysis of the arrester operation, which has design features of flow ultrasound generators. The paper includes both experimental investigations of the proposed spark gap arrester design and mathematical modeling of the processes occurring in it. A description of the methods used is presented, and a comparison of the time dependences of currents and voltages obtained as a result of experimental studies and as a result of calculations is performed in the next section. The calculation results include oscillograms of the voltage and current, and the plasma temperature distribution in the arrester chamber at different moments of time. The investigations show that the presence of a nozzle and resonator leads to an intensification of the gas dynamic effect on the electric arc. [ABSTRACT FROM AUTHOR]

This work presents a characterisation model for the temperature distribution at different substrate depths during the atmospheric plasma spray (APS) coating process. The torch heat flow in this model is simulated as forced convection defined by a surface, a temperature profile, and a convection coefficient. The simulation model considers three plasma temperature profiles of the Al2O3 coating on a 5 mm thickness flat aluminium substrate. The simple and low-cost experimental procedure, based on a thermocouple, measures the plasma plume temperature distribution of the APS coating system, and their results are used to obtain the parameter values of each of the three proposed plasma temperature profiles. The experimental method for in situ non-contact temperature measurements inside the substrate is based on an infrared pyrometry technique and validates the simulation results. The Gaussian temperature profile shows excellent accuracy with the measured temperatures. The Gaussian approach could be a powerful tool for predicting residual stress through a coupled one-way thermal-mechanical analysis of the APS process. [ABSTRACT FROM AUTHOR]

In this study, we have investigated the relationship between the plasma temperature in remote laser-induced breakdown spectroscopy (LIBS) experiments and the hydrophobic properties of silicone rubber insulators (SIRs). Contact angle and LIBS measurements were conducted on both artificially-aged (accelerated aging) and field-aged SIRs. This study reveals a clear connection between plasma temperature and the properties of aged SIRs on artificially-aged SIR specimens. Specifically, the plasma temperature exhibits a consistent increase with the duration of the accelerated aging test. The hydrophobicity of the artificially-aged SIRs was assessed by performing contact angle measurements, revealing a decrease in the hydrophobicity with increased aging test duration. Furthermore, we extended our investigation to the study of nine field-aged SIRs that had been in use on 150 kV overhead transmission lines for 0 to 21 years. We find that the laser absorption and hardness of the material do not relate to the plasma temperature. In summary, we observe a direct connection of plasma temperature to both contact-angle measurements and operation time of the in-service insulators. These results strongly suggest the potential use of LIBS for remotely evaluating the hydrophobicity and aging degree of silicone rubber insulators, thus assessing their real-time on-site operational quality. [ABSTRACT FROM AUTHOR]

One of the most significant medicinal plants used to treat numerous illnesses is Swertia chirayita. The present study demonstrated the compositional analysis of the Swertia chirayita (S. chirayita) plant using an emerging and non-destructive laser-induced breakdown spectroscopy (LIBS) technique. Mg, Ca, K, Fe, Sr, Cr, and Na were verified as necessary elements by the optical emission investigations, while Al, Ti, Si, Ba, Mn, and Li were non-essential. Using the Boltzmann plot technique with stark broadening parameters, plasma temperature and electron number density were calculated in the range of (10,000–12,000) K ±1000 K and (1.5–1.8) × 1017 cm-3, respectively. Finally, compositional analysis was carried out using calibration-free (CF-LIBS) analysis and results were compared with ICP-MS. It was observed that the concentration of Ca and Fe is higher than other detected elements. All the toxic elements are found to be within the safe limit. So, this medicinal plant can be used to cure a variety of diseases that arise due to the deficiency of these elements. [ABSTRACT FROM AUTHOR]

Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Laser propagation in an electron-positron-ion (EPI) plasma medium having a constant density gradient is studied for the soliton evolution in the presence of a parallelly applied strong magnetic field. In a nonlinear regime, laser-plasma interaction forms an electron density hill that moves backward due to the density gradient. This hill localizes laser energy by the mechanism of resonance absorption (ωl = ωpe) and remains stable for a longer period, qualifying for the evolution of electromagnetic soliton. The position of the soliton from where it starts moving backward depends on the slope of the linear density variation. If the slope increases, then the position of the soliton is closer to the plasma boundary (x = 0). Another interesting property of the soliton is that its height decreases, and width increases as the temperature of plasma species rises. We simulate this phenomenon of electromagnetic soliton evolution in 1-D and 2-D by using Particle-In-Cell (PIC) simulation using the EPOCH-4.17.10. [ABSTRACT FROM AUTHOR]

The magnetospheric plasma (hot and thin) and the solar wind plasma (cold and dense) are separated by the Earth's magnetopause, in which plasmas of both origins coexist. Different types of plasma diffusions are found due to this plasma mixing, and kinetic Alfvén solitary waves (KASWs) are one of them. In this work, a theoretical approach is taken to study the fundamental properties of heavy ion acoustic KASWs (HIAKASWs) in a magnetized plasma system whose constituents are nonextensive q-distributed two temperature electrons with dynamical heavy ions. The perturbations of the magnetized collisionless plasma system are investigated using the reductive perturbation technique to deduce the Korteweg–de Vries (K–DV) and modified K–DV (MK–DV) equations to determine the fundamental characteristics of small, but finite amplitude HIAKASWs. The presence of nonextensive electrons, magnetic field, obliquity angle (the angle between the external magnetic field and wave propagation), plasma particle number densities, and the temperature of various plasma species are observed to significantly alter the fundamental properties of HIAKASWs. The findings of our present study may be useful for comprehending the nonlinear wave properties in diverse interstellar plasma environments. [ABSTRACT FROM AUTHOR]

Singly charged background argide ions ArH+, ArN+, ArO+, and Ar2+ create strong spectral interferences in elemental and isotopic analysis by inductively coupled plasma mass spectrometry (ICP MS). In this study, we experimentally investigated the behavior of these ions depending on variations in high-frequency plasma power and argon carrier gas flow rate. Theoretical analysis was performed using thermodynamic modeling to predict the behavior of these argide ions on changes in plasma temperature and argon flow rate. Common patterns in the intensity of these major background ions and changes in their formation efficiency at different working parameters of the ICP are noted. A good agreement between the observed experimental dependences and those derived from thermodynamic models is noted. [ABSTRACT FROM AUTHOR]

There is a lack of information about transforming growth factor beta-1 (TGF-β1) and cytokines contained in pure platelet-rich plasma (P-PRP) and release from pure-platelet-rich gel supernatants (P-PRGS) might be affected by the temperature and time factors; P-PRP from 6 heifers was activated with calcium gluconate. Thereafter, P-PRG and their supernatants (P-PRGS) were maintained at −80, −20, 4, 21, and 37 °C and collected at 3, 6, 12, 24, 48, 96, 144, 192, 240, and 280 h for subsequent determination of TGF-β1, tumor necrosis factor alfa (TNF-α), interleukin (IL)-2, and IL-6; TGF-β1 concentrations were significantly (p < 0.05) higher in PRGS maintained at 21 and 37 °C when compared to PRGS maintained at 4, −20, and −80 °C; PRGS TNF-α concentrations were not influenced by temperature and time factors. However, PRGS maintained at 4 °C showed significantly (p < 0.05) higher concentrations when compared to PRGS maintained at −20, and −80 °C at 144, and 192 h. IL-6 concentrations were significantly (p < 0.05) higher in PRGS stored at −20, and −80 over the first 48 h and at 10 days when compared to PRGS stored at 4, 21, and 37 °C. These results could suggest that P-PRP/P-PRGS could be maintained and well preserved for at least 12 days at room temperature for clinical use in bovine therapeutic massive protocols. [ABSTRACT FROM AUTHOR]

This work explores microcombustion technologies enhanced by plasma-assisted combustion, focusing on a novel simulation model for a Y-shaped device with a non-premixed hydrogen-air mixture. The simulation integrates the ZDPlasKin toolbox to determine plasma-produced species concentrations to Particle-In-Cell with Monte Carlo Collision analysis for momentum and power density effects. The study details an FE-DBD plasma actuator operating under a sinusoidal voltage from 150 to 325 V peak-to-peak and a 162.5 V DC bias. At potentials below 250 V, no hydrogen dissociation occurs. The equivalence ratio fitting curve for radical species is incorporated into the plasma domain, ensuring local composition accuracy. Among the main radical species produced, H reaches a maximum mass fraction of 8% and OH reaches 1%. For an equivalence ratio of 0.5, the maximum temperature reached 2238 K due to kinetic and joule heating contributions. With plasma actuation with radicals in play, the temperature increased to 2832 K, and with complete plasma actuation, it further rose to 2918.45 K. Without plasma actuation, the temperature remained at 300 K, reflecting ambient conditions and no combustion phenomena. At lower equivalence ratios, temperatures in the plasma area consistently remained around 2900 K. With reduced thermal power, the flame region decreased, and at Φ = 0.1, the hot region was confined primarily to the plasma area, indicating a potential blow-off limit. The model aligns with experimental data and introduces relevant functionalities for modeling plasma interactions within microcombustors, providing a foundation for future validation and numerical models in plasma-assisted microcombustion applications. [ABSTRACT FROM AUTHOR]

The paper presents the results of the study of tungsten surface structure modification under helium plasma irradiation. It is revealed that during irradiation of samples, surface modification in the form of relief development as a result of irradiation is observed. X‐ray phase analysis showed that no new phases of the W system were found after irradiation, only an increase in the intensity of diffraction lines was observed. There are significant differences in the microstructure of tungsten depending on the temperature of helium plasma irradiation in the above range. It is assumed that the cause of defects is the extremely low solubility of helium in tungsten. Metallographic analysis has shown that at irradiation of tungsten samples in time modes 1 and 1.5 hours, the degree of relief development is not high in comparison with the tungsten sample irradiated in the 0.5‐hour mode. The greatest increase in surface roughness of the sample irradiated for 1 hour was registered, which is associated with the formation of small cracks, bubbles, and pores in the surface layer. At the same time, in the samples irradiated for 1.5 hours, the surface of which is characterized by chaotically located protrusions and depressions of various shapes, the roughness parameter Ra was 0.0603 μm. It was found that at temperature regimes T = 1200°C and T = 1500°C, the microhardness of tungsten increases by 10% and 11%, respectively. [ABSTRACT FROM AUTHOR]

Immunodetection of cardiac isoforms of troponin I (cTnI) and troponin T (cTnT) in blood samples is widely used for the diagnosis of acute myocardial infarction. The cardiac troponin complex (ITC-complex), comprising cTnI, cTnT, and troponin C (TnC), makes up a large portion of troponins released into the bloodstream after the necrosis of cardiomyocytes. However, the stability of the ITC-complex has not been fully investigated. This study aimed to investigate the stability of the ITC-complex in blood samples. A native ITC-complex was incubated in buffer solutions, serum, and citrate, heparin, or EDTA plasma at various temperatures. Western blotting and gel filtration were performed, and troponins were detected using specific monoclonal antibodies. The ITC-complex dissociated at 37 °C in buffers with or without anticoagulants, in citrate, heparin, and EDTA plasmas, and in serum, into a binary cTnI-TnC complex (IC-complex) and free cTnT. In plasma containing heparin and EDTA, the IC-complex further dissociated into free TnC and cTnI. No dissociation was found at 4 °C or at room temperature (RT) in all matrices within 24 h except for EDTA plasma. After incubation at 37 °C in EDTA plasma and serum, dissociation was accompanied by proteolytic degradation of both cTnI and cTnT. The presence of anti-troponin autoantibodies in the sample impeded dissociation of the ITC-complex. The ITC-complex dissociates in vitro to form the IC-complex and free cTnT at 37 °C but is mostly stable at 4 °C or RT. Further dissociation of the IC-complex occurs at 37 °C in plasmas containing heparin and EDTA. [ABSTRACT FROM AUTHOR]

The study considers a possibility of studying the manifestation of the major background ions derived from the main elements (H, N, O, and Ar) of inductively coupled plasma under low-temperature ("cold") plasma conditions through thermodynamic simulation. These ions, known to induce significant spectral interferences, are always observed when aqueous samples are injected into inductively coupled plasma mass spectrometers (ICP–MS). Using thermodynamic simulation in the temperature range from 2000 to 5000 K, the quantitative composition of the major background ions in ICP–MS was determined as a function of plasma temperature. A comparison of the theoretical calculations and experimental data from mass spectral measurements of the major background ions was conducted, revealing a high degree of correlation between the two sets of the results. This agreement between the calculations and experiments confirms the validity of the thermodynamic model used for thermochemical processes in ICP–MS and its applicability to subsequent calculations in addressing analytical challenges. Additionally, a method is proposed for the unambiguous evaluation of the gas kinetic temperature of the plasma, while simultaneously considering practically all major background ions. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To assess the temperature stability of an autodefrost freezer commonly used in veterinary practices, whether the use of a Styrofoam cooler within the freezer provides temperature stability, and the ease of use of a remote monitoring system for the notification of temperature elevations. ANIMALS: None. METHODS: Temperature in the freezer and 2 Styrofoam coolers were assessed with remote monitoring thermometers every 15 minutes. Temperature values were monitored from October 11 to December 18, 2023 (for a 68-day period). Data analysis focused on temperatures for the freezer exceeding 0 °C and the elevations in temperatures within the coolers relative to the freezer. RESULTS: The freezer had an increase in temperature approximately every 16 hours. Over 68 days, the freezer had a temperature greater than 0 °C 27 times, representing 26 separate elevations. The Styrofoam coolers within the freezer never registered a temperature higher than -5 °C. Elevations in temperature within the freezer were larger in magnitude than temperature elevations within the coolers, which showed smaller-magnitude changes in temperature. [ABSTRACT FROM AUTHOR]

Titanium tetrachloride (TiCl4) is a well-known source of titanium (Ti) for the formation of titanium nitride (TiN) barrier material in the semiconductor interconnection process; however, the reaction of by-products with airborne molecules can cause unexpected pump trips and equipment breakdown from the by-product powder build-up. Plasma scrubbers are used to decompose by-products, but hydrogen chloride (HCl) and nitrogen oxides are produced during and after the process. The process mechanisms change when the temperature and applied power of the heat source change. In this paper, we study the influence of the reactor temperature and applied power to the heat source on the decomposition capacity of TiCl4 in a plasma pretreatment system (PPS). We examine the effect of the temperature and heat source power to understand the reaction mechanisms for the composition and decomposition of gaseous species with chemical reactions through simultaneous methods. We analyzed the system with computational fluid dynamics (CFD) and chemical kinetic simulation to investigate the changes of the system mechanism. Subsequently, we achieved results for the correlation between the temperature of the reactor, power applied to the heat source, composition and decomposition of species, and chemical reaction mechanisms. [ABSTRACT FROM AUTHOR]

An edge-confined single-species plasma will relax to create a potential energy hill that climbs from the boundary. This hill represents a potential well for species of the opposite sign and can be a means to confine the second species. With this ultimate application in mind, we have studied the relation between the plasma temperature, the number of confined particles, and the electrostatic potential well that forms in a fully non-neutral plasma of electrons in a trapping volume with an artificially structured boundary (ASB). An ASB is a structure that produces periodic short-range static electric and magnetic fields for confining a plasma. To perform a detailed analysis on this topic, simulations using a particle-in-cell code have been performed. By varying the configurational elements of the ASB, such as the bias on the boundary electrodes and the internal radius of the structure, coupled with a course thermalization process and a prescribed threshold for particle leakage, potential well values were determined for a range of plasma temperatures and confinement conditions. Maximum well depths were observed below a threshold plasma temperature in each configuration. This study gives insight into the limitations of primary particle confinement with this type of structure and optimal conditions for the formation of a potential well that might be utilized to confine a second species. [ABSTRACT FROM AUTHOR]

This paper focuses on the theoretical study of the burning rate of D-T fuel in Z-pinch devices with magneto-inertial confinement. The investigated nuclear fusion process involved fast laser ignition of a mixed D-T fuel contained in a capsule at a temperature of 10 keV, influenced by a strong electromagnetic field. The D-T, D-D, D-3He, 3He-3He, and T-T fusion reactions were employed in the calculations. Based on modern experimental fit data of nuclear fusion reaction rates, the particle and energy balance equations, along with their numerical solutions, were considered, utilizing the ion densities of charged particles such as protons, deuterium, tritium, 3He, and 4He ions. The plasma was in a hot, ultra-dense state, under the quasi-neutrality condition, with initial deuterium and tritium densities of 5 × 10 23 cm−3 and an electron density of 10 × 10 23 cm−3. The ion and electron temperatures were considered equal in this paper. The time dependencies of the ion densities, plasma temperature, energy yield from charged ions and neutrons, fusion power density, and bremsstrahlung radiation loss were investigated. [ABSTRACT FROM AUTHOR]

Calcium silicate ceramics were successfully prepared at various temperatures by spark plasma sintering technique (SPS) using two different compositions, and their morphological and structural characteristics were thoroughly analyzed and compared by scanning electron microscope, X‐ray diffraction, and apparent density measurements. The effect of the sintering method and temperature on microstructural properties has also been studied. The sintered samples prepared by SPS through a heat input of 800°C showed the highest densification as results 800°C was suggested as optimum sintering temperature. Different phases presented in pseudo‐wollastonite, wollastonite, and triclinic Ca3(SiO4)O have been observed in the case of 50C50C with lowest porosity ∼6%$\sim \;6\% $ and highest density 2.6 g/cm3, whereas the composition 10C90S resulted in a fully amorphous material presented in granular microstructure with density of 1.7 g/cm3. [ABSTRACT FROM AUTHOR]

Inonotus obliquus, a medicinal fungus, has garnered significant attention in scientific research and medical applications. In this study, protoplasts of the I. obliquus HS819 strain were prepared using an enzymatic method and achieved a regeneration rate of 5.83%. To enhance polysaccharide production of I. obliquus HS819, atmospheric and room temperature plasma (ARTP) technology was employed for mutagenesis of the protoplasts. Through liquid fermentation, 32 mutant strains exhibiting diverse characteristics in morphology, color of the fermentation broth, mycelial pellet size, and biomass were screened. Secondary screening identified mutant strain A27, which showed a significant increase in polysaccharide production up to 1.67 g/L and a mycelial dry weight of 17.6 g/L, representing 137.67% and 15% increases compared to the HS819 strain, respectively. Furthermore, the fermentation period was reduced by 2 days, and subsequent subculture cultivation demonstrated stable polysaccharide production and mycelial dry weight. The genome resequencing analysis of the HS819 strain and mutant strain A27 revealed 3790 InDel sites and mutations affecting 612 functional genes associated with polysaccharide synthesis. We predict that our findings will be helpful for high polysaccharide production through genetic engineering of I. obliquus. [ABSTRACT FROM AUTHOR]

Agar oligosaccharides from the degradation of agar harbor great potential in the food and pharmaceutical industries. An agar-degrading bacterium, Vibrio natriegens WPAGA4, was isolated from the deep sea in our previous work. However, the agar-degrading activity of WPAGA4 remains to be improved for more production benefits of this strain. The aim of this study was to enhance the agar-degrading activity of WPAGA4 by using atmospheric and room temperature plasma (ARTP) mutagenesis. Three mutant strains, including T1, T2, and T3, with good genetic stability were obtained, and the agar-degrading activities of these strains increased by 136%, 141%, and 135%, respectively. The optimal temperature and pH for agar degradation were slightly changed in the mutant strains. No sequence mutation was detected in all the agarase genes of WPAGA4, including agaW3418, agaW3419, agaW3420, and agaW3472. However, ARPT mutagenesis increased the relative expression levels of agaW3418, agaW3419, and agaW3420 in the mutant strains, which could be the reason for the improvement of degradation activities in the mutant strains. Furthermore, T3 had the lowest consumption rate of agar oligosaccharide, which was 21% less than the wild-type strain. Therefore, T3 possessed a preferable production value due to its higher degrading activity and lower consumption of agar oligosaccharides. The current work enhanced the agar-degrading activity of WPAGA4 and offered strains with greater potential for agar oligosaccharide production, thereby laying the foundation for industrial applications. [ABSTRACT FROM AUTHOR]

We present measurements of the 2p-3d transition opacity of a hot molybdenum–scandium sample with nearly half-vacant molybdenum M-shell configurations. A plastic-tamped molybdenum–scandium foil sample is radiatively heated to high temperature in a compact D-shaped gold Hohlraum driven by ∼30 kJ laser energy at the SG-100 kJ laser facility. X rays transmitted through the molybdenum and scandium plasmas are diffracted by crystals and finally recorded by image plates. The electron temperatures in the sample in particular spatial and temporal zones are determined by the K-shell absorption of the scandium plasma. A combination of the IRAD3D view factor code and the MULTI hydrodynamic code is used to simulate the spatial distribution and temporal behavior of the sample temperature and density. The inferred temperature in the molybdenum plasma reaches a average of 138 ± 11 eV. A detailed configuration-accounting calculation of the n = 2–3 transition absorption of the molybdenum plasma is compared with experimental measurements and quite good agreement is found. The present measurements provide an opportunity to test opacity models for complicated M-shell configurations. [ABSTRACT FROM AUTHOR]

Background: Human lysozyme (hLYZ) is a natural antibacterial protein with broad applications in food and pharmaceutical industries. Recombinant production of hLYZ in Komagataella phaffii (K. phaffii) has attracted considerable attention, but there are very limited strategies for its hyper-production in yeast. Results: Here through Atmospheric and Room Temperature Plasma (ARTP)-based mutagenesis and transcriptomic analysis, the expression of two genes MYO1 and IQG1 encoding the cytokinesis core proteins was identified downregulated along with higher hLYZ production. Deletion of either gene caused severe cytokinesis defects, but significantly enhanced hLYZ production. The highest hLYZ yield of 1,052,444 ± 23,667 U/mL bioactivity and 4.12 ± 0.11 g/L total protein concentration were obtained after high-density fed-batch fermentation in the Δmyo1 mutant, representing the best production of hLYZ in yeast. Furthermore, O-linked mannose glycans were characterized on this recombinant hLYZ. Conclusions: Our work suggests that cytokinesis-based morphology engineering is an effective way to enhance the production of hLYZ in K. phaffii. [ABSTRACT FROM AUTHOR]

ZrB 2 -based composite coatings with addition of Al 2 TiO 5 were prepared via very low-pressure plasma spraying using mechanically mixed powder of ZrB 2 and Al 2 O 3 -40 wt%TiO 2 (AT40). The ablation behavior of the composite coating was investigated using a high temperature plasma jet at a temperature of ∼2500oC. Results demonstrate the addition of AT40 improved the ablation resistance of the ZrB 2 coatings at high temperatures. The coating with 17 vol%AT40 addition exhibited the lowest ablation rate, approximately 1.33 μm/s, which is only one-third of that of the pure ZrB 2 coating. The added AT40 filled the pores between ZrO 2 crystals during ablation, reducing oxygen penetration and lowering the ablation rate. However, excessive addition of AT40 hindered gaseous B 2 O 3 release and thus led to large pore formation, impairing the protective effect. The ZrB 2 -AT40 coating is a promising candidate for high-temperature applications. [ABSTRACT FROM AUTHOR]

To investigate the influences of Cr2AlC mass fraction and supersonic plasma spraying process on the microstructure and mechanical properties of Cr2AlC reinforced 410 stainless steel composite coatings, the coatings containing different mass fractions of Cr2AlC were prepared and investigated. The composite coating exhibited low porosity and high adhesion strength. The addition of Cr2AlC significantly enhanced the hardness of the composite coatings through particle strengthening. However, when the mass fraction of Cr2AlC was 20%, the aggregation of Cr2AlC resulted in a strong decrease in the coating preparation efficiency, as well as a decline in adhesion strength. In the supersonic plasma spraying process, the Ar flow rate mainly influenced the flight velocity of the particles, while the H2 flow rate and the current mainly affected the temperature of the plasma torch. Consequently, all of them influenced the melting degree of particles and the quality of the coating. The lowest porosity and the highest hardness and adhesion strength could be obtained when the Ar flow rate is 125 L/min, the H2 flow rate is 25 L/min, and the current is 385 A. [ABSTRACT FROM AUTHOR]

Plasma electrolytic oxidation (PEO) is normally carried out under conditions with electrolyte cooling. However, the effect of the temperature of the electrolytes on the PEO behavior and properties of the resulting coatings is seldom investigated. In this study, PEO of pure Al was carried out in a dilute aluminate electrolyte with the electrolyte temperature being controlled under low (~10–30 °C), medium (~40–50 °C) and high (~70–80 °C) temperature ranges, respectively. The morphology, microstructure, composition and phase component of the coatings fabricated under the different temperature ranges were analyzed by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD). The corrosion resistances of the coatings were evaluated by electrochemical methods. The hardness of the coatings and substrate following the PEO treatment in the different electrolyte temperature ranges were also tested. It was found that a higher electrolyte temperature resulted in a higher growth rate and rougher coatings. Moreover, the α-Al2O3 content was reduced as the electrolyte temperature increased. The highest corrosion resistance was registered for the coating obtained under the lowest temperature range, whereas the corrosion resistance of the coating obtained under the highest temperature range was the worst. The higher growth rate under high electrolyte temperatures was associated with the increased kinetics of the PEO reactions. However, the temperature of the electrolyte should be controlled under a suitable range to ensure reasonable coating properties. [ABSTRACT FROM AUTHOR]

For low costs, numerical simulation is an effective method to reveal the internal mechanisms inside a thermal plasma torch. Various simulation models for studying the inside or outside characteristics of thermal plasma torches have been built and discussed. However, to reveal the inside mechanisms of thermal plasma torches under various working conditions to support the materials processing, more attention should be paid to building precise models of laminar plasma torches. Thus, based on the user-defined function (UDF) and user-defined scalar (UDS) of ANSYS Fluent software, the assumptions, governing equations, boundary conditions, and solving method were discussed in detail, and a corresponding numerical model of a homemade laminar plasma torch was first built. For verifying the effectiveness of the proposed numerical model and studying the influence of the gas components on the arc characteristics, the working conditions and experimental setups were introduced in sequence. Finally, the numerical and experimental results of the homemade laminar plasma torch were obtained and discussed in detail. The study results show that: ① The axial temperature of the plasma torch could be divided into three sections along the axis: peak temperature area (10 mm < x < 20 mm), stable temperature area (20 mm < x < 62 mm) and decrease temperature area (62 mm < x < 95 mm). Under the same input conditions, when pure argon gas was used, the peak temperature at the outlet was reached at approximately 7590 K, while for pure nitrogen and 50%Ar + 50%N2, the corresponding peak temperatures were 6785 K and 7402.2 K, respectively. ② The axial velocity of pure nitrogen is much higher than that of pure argon and 50%Ar + 50%N2, while that of pure argon and 50%Ar + 50%N2 has little difference. In addition, when nitrogen gas was used, the peak velocity at the outlet reached 185 m/s, whereas, for argon gas and 50%Ar + 50%N2, the corresponding peak velocities were 146 m/s and 169 m/s, respectively. ③ The simulated arc voltage trends under different working conditions are well in accordance with the experimental arc voltage trends. [ABSTRACT FROM AUTHOR]

Herbig Ae/Be (HAeBe) stars are intermediate-mass pre-main-sequence stars, characterized by infrared (IR) excess and emission lines. They are observed to emit X-rays, whose origin is a matter of discussion and not settled yet. X-ray emission is not expected in HAeBe stars, as they lack the subsurface convective zone. In this study, we retrieved observations from the Chandra archive for 62 HAeBe stars, among which 44 sources (detection fraction ∼71  per cent) were detected in X-rays, with 7 being new detections. We use this sample as a test bed to conduct a comparative analysis of the X-ray properties of HAeBe stars and their low-mass counterparts, T Tauri stars (TTSs). Further, we compare the X-ray properties of HAeBe stars and TTSs with optical and IR properties to constrain the X-ray emission mechanism in HAeBe stars. We found no correlation between X-ray emission and disc properties of HAeBe stars, confirming that X-rays are not related to accretion shocks. About 56  per cent of HAeBe stars without any known subarcsec companions have lower plasma temperatures (kT ≤ 2 keV). We observe flaring/variability in HAeBe stars with confirmed low-mass companions. These stars show plasma temperatures > 2 keV, similar to TTSs. Guided by this information, we discuss the role of a T Tauri companion for X-ray emission seen in our sample of HAeBe stars. From the results obtained in this paper, we suggest that X-ray emission from HAeBe stars may not be related to accretion shocks or hidden TTS, but rather can be due to magnetically driven coronal emission. [ABSTRACT FROM AUTHOR]

We study the loop oscillations after a solar flare on 19 January 2023, in the active region N11E40 3196, which is well observed by the SDO/AIA. After tracing the loop position and fitting, we find that the loop oscillations have a period between 3 and 9 min at various locations, such as from the leg to the top or from the inner to the outer loop. Their oscillating amplitudes decrease with time. Two loops display the position oscillation simultaneously with their brightness oscillation. After the analysis of the differential emission measure (DEM), we find that two of their loop position oscillations resulted from the plasma density fluctuation. Meanwhile, it is interesting that the brightness of these two position oscillations displays a typical period of about 4 min, similar to that of the position oscillation. This is possible due to both the plasma density and temperature fluctuation there. Our findings provide the physical clues for studying and understanding the mechanism of the loop position and brightness oscillations. [ABSTRACT FROM AUTHOR]

Saccharomyces cerevisiae D65 isolated from the Malus asiatica naturally fermented liquid was used as the parental strain for mutation breeding of atmospheric and room temperature plasma (ARTP). The mutant strains with excellent characteristics were obtained through acid resistance test, esterase activity test, and fermentation test multi-step screening. The results showed that the specific activity of total esterase of D65-4D10 was 2428.30 U·g−1, and the ester content in the fermented Malus asiatica wine was 5710.75 ug·L−1, which was 20.69% and 41.64% higher than that of the parental strain, respectively. Moreover, the Malus asiatica wine fermented by D65-4D10 had stronger antioxidant capacity than the control, the highest score in sensory evaluation, and completed fermentation 24 h earlier than commercial yeast. After seven generations of continuous culture, the fermentation performance of D65-4D10 remained stable and had the potential to be applied to Malus asiatica wine fermentation. [ABSTRACT FROM AUTHOR]

The wavelengths and transition rates of W40+-W42+ ions within the range of 40–140 Å, have been calculated using the Flexible Atomic Code of the Dirac-Fock-Slater method with a central potential. We investigated the charge state distribution of W38+-W45+ ions at different temperatures by constructing an appropriate rate equation and demonstrate the importance of the dielectronic recombination process. Additionally, we simulated the emission spectra of W40+-W42+ ions in a Tokamak plasma environment using collisional-radiative modeling. Our findings demonstrate strong agreement with experimental results and other related theoretical investigations. Finally, we propose certain pairs of transition lines as diagnostic tools for plasma temperature and density, leveraging the correlation between line intensity ratio and electron temperature and density. [ABSTRACT FROM AUTHOR]

G352.7−0.1 is a mixed-morphology (MM) supernova remnant (SNR) with multiple radio arcs and has a disputed supernova origin. We conducted a spatially resolved spectroscopic study of the remnant with XMM–Newton X-ray data to investigate its explosion mechanism and explain its morphology. The global X-ray spectra of the SNR can be adequately reproduced using a metal-rich thermal plasma model with a temperature of ∼2 keV and ionization time-scale of ∼3 × 1010 cm−3 s. Through a comparison with various supernova nucleosynthesis models, we found that observed metal properties from Mg to Fe can be better described using core-collapse supernova models, while thermonuclear models fail to explain the observed high Mg/Si ratio. The best-fit supernova model suggests a ∼13 M⊙ progenitor star, consistent with previous estimates using the wind bubble size. We also discussed the possible mechanisms that may lead to SNR G352.7−0.1 being an MMSNR. By dividing the SNR into several regions, we found that the temperature and abundance do not significantly vary with regions, except for a decreased temperature and abundance in a region interacting with molecular clouds. The brightest X-ray emission of the SNR spatially matches with the inner radio structure, suggesting that the centrally filled X-ray morphology results from a projection effect. [ABSTRACT FROM AUTHOR]

Predicting the temperature in the graphite purification zone of arc plasma is not only useful for guiding the smoothness of the production process, which affects the final quality of the graphite product, but also provides decision support for the implementation of fine temperature control. This study conducted an analysis of nine variables associated with the temperature of the graphite purification zone within arc plasma to develop temperature prediction models. Using the random forest algorithm for feature selection, four key variables were identified with importance exceeding 0.4. These variables include the main gas flow rate, powder flow rate, voltage, and current. Subsequently, three different algorithms, namely Error Back Propagation (BP), Extreme Learning Machine (ELM), and Long Short-Term Memory (LSTM), were employed to develop various models for predicting temperature. Ultimately, the predictive performance of the models was assessed by comparing the temperature prediction models for various operating scenarios using different evaluation criteria. The experimental results show that the three models have their own advantages and disadvantages in different working conditions. The LSTM model exhibited superior predictive performance in C1~C2 and C6~C9 working conditions, the ELM model demonstrated superior predictive performance in C3~C4 working conditions, and the BP model demonstrated superior predictive performance in C5 working conditions. Hence, in the actual purification process, a variety of prediction models can be used in combination to facilitate the continuous monitoring of temperature variations in the graphite purification zone. [ABSTRACT FROM AUTHOR]

This work used spectroscopic plasma emission analysis to investigate the parameters of ZnTi plasma formed in the atmosphere by a Q-switched Nd:YAG nanosecond laser system emitting infrared (1064 nm) laser light with an energy output spanning 200-500 mJ. The spectrometer was used to examine and record the emission spectrum profiles of (ZnTi), which helped to extract crucial plasma characteristics (i.e., T e and n e). The electron density was measured using the Stark broadening, and the plasma temperature was calculated using the Boltzmann plot technique. The electron temperature was between 2.04 and 2.15 eV, and the electron density was between (15.72 and 18.99) × 10 1 6 cm − 3 . The Debye length (l) , the number of particles in the Debye sphere ( N d) , and the plasma frequency ( f p) can all be estimated from the electron temperature ( T e) and the electron density ( n e) ( f p). [ABSTRACT FROM AUTHOR]

The paper proposes an explanation of the physicochemical processes occurring during the electric-arc plasma-chemical synthesis of carbon nanomaterials. A diagram of the action of forces on charged particles and a diagram of forces acting on the motion of ions in an arc in the presence of a magnetic field for the plasma-chemical synthesis of carbon nanomaterials are presented and considered. The levels of organization of matter in an arc discharge during the formation of carbon vapour with an increase in temperature are considered. A comparative characterization and a conditional scheme for the formation of various carbon nanostructures during plasma-chemical synthesis are proposed. The sequence of carbon transformations during the formation of carbon nanomaterials and the conditional levels of organization of matter with the types of processes occurring on each of them during structure formation, as the classification of carbon structures by size levels under conditions of temperature decrease from plasma temperature to room one are established. Carbon nanomaterials are synthesized by the plasma-chemical method, and the analysis of the obtained and processed carbon nanostructures is carried out by the following methods: scanning electron microscopy, transmission electron microscopy, Raman spectral analysis, UV–VIS spectroscopy, spectrophotometric analysis. The presence of fullerenes in the products of plasma-chemical synthesis is established, and fullerenes are extracted from the synthesized wall soot. [ABSTRACT FROM AUTHOR]

In this paper, we first briefly describe the constructed Inductively Coupled Plasma Source device, ICP-13.56, which includes two vacuum and atmospheric pressure torches. Afterward, focusing on the vacuum-torch operation, we discuss the plasma temperature characterization and examine the phenomenon of E to H mode-transition during the variation of experiment parameters including the initial pressure and input RF power. Based on the spectroscopic measurements of argon gas at pressure 4 mbar and input RF power 400 W, the electron excitation temperature of 11636 °K was obtained using the Boltzmann line method. Additionally, we have calculated the absorbed power in terms of plasma density and gas pressure using equations governing electromagnetic fields in ICP plasma. According to these relations, behaviors of load characteristics have been analyzed and E to H mode transition has been identified.

In this study, we have investigated the relationship between the plasma temperature in remote laser-induced breakdown spectroscopy (LIBS) experiments and the hydrophobic properties of silicone rubber insulators (SIRs). Contact angle and LIBS measurements were conducted on both artificially-aged (accelerated aging) and field-aged SIRs. This study reveals a clear connection between plasma temperature and the properties of aged SIRs on artificially-aged SIR specimens. Specifically, the plasma temperature exhibits a consistent increase with the duration of the accelerated aging test. The hydrophobicity of the artificially-aged SIRs was assessed by performing contact angle measurements, revealing a decrease in the hydrophobicity with increased aging test duration. Furthermore, we extended our investigation to the study of nine field-aged SIRs that had been in use on 150 kV overhead transmission lines for 0 to 21 years. We find that the laser absorption and hardness of the material do not relate to the plasma temperature. In summary, we observe a direct connection of plasma temperature to both contact-angle measurements and operation time of the in-service insulators. These results strongly suggest the potential use of LIBS for remotely evaluating the hydrophobicity and aging degree of silicone rubber insulators, thus assessing their real-time on-site operational quality.

We study kink oscillations of the system of two parallel magnetic tubes in the presence of plasma cooling. We assume that the characteristic cooling time is much larger than the characteristic time of kink oscillations. Using the ratio of two characteristic times as a small parameter, we derive the expression for the adiabatic invariant, which is a quantity that remains constant during the cooling process. Then, we study in detail a particular case where the plasma densities in the two tubes are the same, the plasma temperature outside of the tube does not change, and the plasma temperature inside the tubes decreases exponentially. We found that cooling causes the increase of the oscillation frequencies and amplitudes. These results are the generalization of similar results previously obtained for a single magnetic tube. We compared the efficiency of amplification of kink oscillations caused by cooling in counteracting the damping of oscillations due to resonant absorption in two models of coronal magnetic loops: monolithic and consisting of two parallel filaments. [ABSTRACT FROM AUTHOR]

In order to evaluate the effect of cutoff criteria on spectral temperature measurement, partition functions are calculated for argon plasma at 3000–30 000 K using three different cutoff criteria (semi-empirical method, NIST data, and Griem's theory). The effects of cutoff criteria on particle number density and plasma temperature measurements were discussed. The results indicate that as the plasma temperature increases, the degree of particle ionization increases and the cutoff effect weakens. The cutoff criteria have little impact on the calculation of the number density of the main components of the plasma but have a significant impact on the calculation of the number density of non-major components. The cutoff criterion using the NIST data can be reliably used for the temperature measurement, especially when the standard temperature method is used. Nevertheless, Griem's theory should be considered for accurate calculation of radiation intensity. [ABSTRACT FROM AUTHOR]

The prevention and treatment of organic pollutants in wastewater have attracted extensive attention because they threaten ecological balance and human health. Traditional wastewater treatment technologies, such as physical, chemical, biological methods, etc., are still limiting and challenging due to some factors, such as high energy consumption, chemical agents, low efficiency, and secondary pollution. At present, gliding arc plasma, based on equilibrium/non-equilibrium in plasma temperature, demonstrates itself to be one of the most promising technologies for the degradation of refractory organics with its fast degradation rates, no secondary contamination, high efficiency, and so on. The theoretical mechanisms and typical applications of gliding arc plasma degradation of wastewater are introduced in detail in this paper. The physical and chemical characteristics of gliding arc plasma and the role of the reactive species in the plasma are reviewed. [ABSTRACT FROM AUTHOR]

This study focuses on optimizing the mutagenesis process for Morchella eximia (Mel-7) mycelia through atmospheric and room temperature plasma (ARTP) mutation and explores the resultant thermal adaptability and physiological responses of mutant strains. This research demonstrated a clear relationship between ARTP mutagenesis exposure duration and lethality rate, indicating that an exposure time of 40 s resulted in the optimal balance of inducing mutations without causing excessive mortality. Additionally, this study established 43 °C as the ideal screening temperature for identifying mutant strains with enhanced heat resistance, as this temperature significantly challenges the mycelia while allowing thermotolerant strains to be distinguishable. Among the screened mutants, strains L21, L23, L44, and L47 exhibited superior growth and high-temperature tolerance, with notable resilience at 30 °C, highlighting their enhanced adaptability to above-optimal temperatures. Furthermore, this research delved into biochemical responses, including lipid peroxidation and non-enzymatic antioxidant content, highlighting the diverse mechanisms, such as enhanced lipid peroxidation resistance and increased antioxidant content, employed by mutant strains to adapt to temperature fluctuations. The activities of antioxidant enzymes, including peroxidase (POD) and superoxide dismutase (SOD), were shown to be significantly influenced by temperature elevations, illustrating their critical roles in the thermal adaptation of mutant strains. These findings shed light on the importance of considering mutation duration and temperature screening in the development of thermotolerant fungal strains with potential applications in various industries. This study's breakthrough lies in its comprehensive understanding of the thermal adaptability of Mel-7 mycelia and the identification of promising mutant strains, offering valuable insights for both academic and industrial purposes. [ABSTRACT FROM AUTHOR]

The considerable expenses associated with carbon fiber (CF) production have imposed limitations on its widespread application across diverse industries, primarily due to the costs of precursor materials and energy−intensive post−treatment procedures. This research explores the potential utilization of Alberta oilsands asphaltenes (AOAs), a carbon−rich by−product derived from oilsands extraction, as a more cost−effective precursor for CF production. Polystyrene and poly(styrene–butadiene–styrene) were also used as polymer additives. In addition to conventional thermal post−treatment, microwave plasma was employed for the carbonization process. The CFs generated through this approach were subjected to a comprehensive analysis involving SEM, FTIR, TGA, XRD, and Raman spectroscopy. The best tensile strength and Young's modulus of the AOA carbon fibers when using conventional thermal post−treatment were 600 MPa and 70 GPa, respectively. The microwave plasma process indicates the higher temperature and promise of eliminating heteroatoms of AOA carbon fibers. The temperature for microwave plasma modelling was set using COMSOLTM, with the modelling temperature and detection temperature being established at 1600 K and 1568 K, respectively. [ABSTRACT FROM AUTHOR]

Spark plasma sintering is a process of rapid, low-temperature, and high-density sintering. Moreover, traditional sintering methods can solve the problems of large grain sizes and low densities. The sintering temperature plays a crucial role in influencing the physical properties of high-silicon–aluminum (Si-Al) composites. This work investigated the impact of temperature on the microstructure, interface, and physical properties of high-Si-Al composites by spark plasma sintering. The results demonstrate that when the powder was processed by ball milling at a sintering temperature of 565 °C, the material exhibited the densest microstructure with minimal pore formation. The average size of the silicon phase is the smallest. The material's thermal conductivity is 134.6 W/m·K, the thermal expansion coefficient is 8.55 × 10−6 K−1, the Brinell hardness is 219 HBW, the density is 2.415 g/cm3, and the density reaches 97.75%. An appropriate sintering temperature facilitates particle rearrangement and dissolution–precipitation processes, enhancing the material structure and performance. [ABSTRACT FROM AUTHOR]

Based on the detailed term accounting approach, the relationship between extreme ultraviolet conversion efficiency and plasma conditions, which range from 5 to 200 eV for plasma temperature and from 4.63 × 1017 to 4.63 × 1022 cm−3 for plasma density, is studied for lithium plasmas through spectral simulations involving very extended atomic configurations, including a benchmark set of autoionizing states. The theoretical limit of the EUV conversion efficiency and its dependence on sustained plasma time are given for different plasma densities. The present study provides the necessary understanding of EUV formation from the perspective of atomic physics and also provides useful knowledge for improving EUV conversion efficiency with different technologies. [ABSTRACT FROM AUTHOR]

(2S)-Naringenin, a dihydro-flavonoid, serves as a crucial precursor for flavonoid synthesis due to its extensive medicinal values and physiological functions. A pathway for the synthesis of (2S)-naringenin from glucose has previously been constructed in Saccharomyces cerevisiae through metabolic engineering. However, this synthetic pathway of (2S)-naringenin is lengthy, and the genes involved in the competitive pathway remain unknown, posing challenges in significantly enhancing (2S)-naringenin production through metabolic modification. To address this issue, a novel high-throughput screening (HTS) method based on color reaction combined with a random mutagenesis method called atmospheric room temperature plasma (ARTP), was established in this study. Through this approach, a mutant (B7-D9) with a higher titer of (2S)-naringenin was obtained from 9600 mutants. Notably, the titer was enhanced by 52.3% and 19.8% in shake flask and 5 L bioreactor respectively. This study demonstrates the successful establishment of an efficient HTS method that can be applied to screen for high-titer producers of (2S)-naringenin, thereby greatly improving screening efficiency and providing new insights and solutions for similar product screenings. [ABSTRACT FROM AUTHOR]

Measurement of the temporal evolution of laser-produced plasma temperature is very important for many of its applications, and several plasma diagnostic tools are routinely used by researchers. However, it is very challenging to measure the properties of the plasma at the early and late times of its evolution using a single diagnostic tool. In this study, we combined emission and laser absorption spectroscopy to compare the excitation temperatures of a laser-produced uranium plasma system. Several U I transitions in the near-infrared spectral range (775–800 nm) were considered, and the Boltzmann plot method was used to measure the excitation temperatures using both emission and absorption spectroscopy. Emission spectroscopy provided early-time temperature measurements of the plasma up to times 2–20 µs, while absorption spectroscopy provided temperature measurements at late times of plasma evolution (for times 5–80 µs). The emission and absorbance of U I transitions were found to follow the Boltzmann distribution, indicating the plasma is likely in the state of local thermodynamic equilibrium even at late times of its lifetime. The emission and absorption-based time-resolved excitation temperatures demonstrated good agreement at earlier times (≤15 µs) in the overlapped temporal region, while a deviation in the measured values was seen at times (≥15 µs), and potential reasons for such a disagreement are discussed. [ABSTRACT FROM AUTHOR]