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5. Additional file 1 of Bioactive isopimarane and 3,4-seco isopimarane diterpenoids from Isodon amethystoides

Author

Zhao, Chenliang, Zhou, Lang, Xie, Wenjian, Zhao, Lihan, Zhang, Chiyuan, He, Kang, Ye, Jianghai, Zhang, Jingjie, Pan, Lutai, Zou, Juan, and Zhang, Hongjie

Abstract

Additional file 1: Figure S1. 1H NMR (600 MHz, CDCl3) spectrum of isoamethinol A (1). Figure S2. 13C NMR (150 MHz, CDCl3) spectrum of isoamethinol A (1). Figure S3. DEPT spectrum of isoamethinol A (1). Figure S4. HSQC spectrum of isoamethinol A (1). Figure S5. HMBC spectrum of isoamethinol A (1). Figure S6. 1H-1H COSY spectrum of isoamethinol A (1). Figure S7. NOESY spectrum of isoamethinol A (1). Figure S8. IR spectrum of isoamethinol A (1). Figure S9. HR-ESI-MS spectrum of isoamethinol A (1). Figure S10. UV spectrum of isoamethinol A (1). Figure S11. 1H NMR (600 MHz, CDCl3) spectrum of isoamethinol B (2). Figure S12. 13C NMR (150 MHz, CDCl3) spectrum of isoamethinol B (2). Figure S13. DEPT spectrum of isoamethinol B (2). Figure S14. HSQC spectrum of isoamethinol B (2). Figure S15. HMBC spectrum of isoamethinol B (2). Figure S16. 1H-1H COSY spectrum of isoamethinol B (2). Figure S17. NOESY spectrum of isoamethinol B (2). Figure S18. IR spectrum of isoamethinol B (2). Figure S19. HR-ESI-MS spectrum of isoamethinol B (2). Figure S20. UV spectrum of isoamethinol B (2). Figure S21. 1H NMR (600 MHz, CDCl3) spectrum of isoamethinol C (3). Figure S22. 13C NMR (150 MHz, CDCl3) spectrum of isoamethinol C (3). Figure S23. DEPT spectrum of isoamethinol C (3). Figure S24. HSQC spectrum of isoamethinol C (3). Figure S25. HMBC spectrum of isoamethinol C (3). Figure S26. 1H-1H COSY spectrum of isoamethinol C (3). Figure S27. NOESY spectrum of isoamethinol C (3). Figure S28. IR spectrum of isoamethinol C (3). Figure S29. HR-ESI-MS spectrum of isoamethinol C (3). Figure S30. 1H NMR (600 MHz, CDCl3) spectrum of isoamethinol D (4). Figure S31. 13C NMR (150 MHz, CDCl3) spectrum of isoamethinol D (4). Figure S32. DEPT spectrum of isoamethinol D (4). Figure S33. HSQC spectrum of isoamethinol D (4). Figure S34. HMBC spectrum of isoamethinol D (4). Figure S35. 1H-1H COSY spectrum of isoamethinol D (4). Figure S36. NOESY spectrum of isoamethinol D (4). Figure S37. IR spectrum of isoamethinol D (4). Figure S38. HR-ESI-MS spectrum of isoamethinol D (4). Figure S39. UV spectrum of isoamethinol D (4). Figure S40. 1H NMR (600 MHz, CDCl3) spectrum of isoamethinol E (5). Figure S41. 13C NMR (150 MHz, CDCl3) spectrum of isoamethinol E (5). Figure S42. DEPT spectrum of isoamethinol E (5). Figure S43. HSQC spectrum of isoamethinol E (5). Figure S44. HMBC spectrum of isoamethinol E (5). Figure S45. 1H-1H COSY spectrum of isoamethinol E (5). Figure S46. NOESY spectrum of isoamethinol E (5). Figure S47. IR spectrum of isoamethinol E (5). Figure S48. HR-ESI-MS spectrum of isoamethinol E (5). Figure S49. UV spectrum of isoamethinol E (5). Figure S50. 1H NMR spectrum of compound 6. Figure S51. 13C NMR spectrum of compound 6.

Published
2022
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8. Two New Cytotoxic Steroidal Alkaloids from Sarcococca Hookeriana.

Author

Huo, Shaojie, Wu, Jichun, He, Xicheng, Pan, Lutai, Du, Jiang, and D'Auria, John C.

Subjects
STEROIDAL alkaloids, CANCER cells, CELL lines, CELL-mediated cytotoxicity, X-ray crystallography
Abstract

Two new steroidal alkaloids, named hookerianine A (1) and hookerianine B (2) were isolated from the stems and roots of Sarcococca hookeriana Baill., along with two known compounds, sarcorucinine G (3) and epipachysamine D (4). On the basis of spectroscopic methods and by comparison with literature data, their structures were determined. As well as X-ray crystallography was performed to confirm compound 4. To identify novel antitumor inhibitors, all compounds were performed a CCK-8 assay against five human cancer cell lines SW480, SMMC-7721, PC3, MCF-7 and K562 in vitro. Compound 2 exhibited moderate cytotoxic activities to all cell lines with IC50 values in the range of 5.97–19.44 μM. Compound 3 was the most effective one against SW480 and K562 cell lines with IC50 values of 5.77 and 6.29 μM, respectively. [ABSTRACT FROM AUTHOR]

Published
2019
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