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18 results on '"Pradhan, Nilotpala"'

7. Biodegradation Of Dibenzothiophene And Its Application In The Production Of Clean Coal

Author

Mishra, Srabani, Pradhan, Nilotpala, Panda, Sandeep, and Akcil, Ata

Subjects
technology, industry, and agriculture, otorhinolaryngologic diseases, respiratory system, complex mixtures, respiratory tract diseases
Abstract

In the current era, the alarming rate at which coal is being burned as a fuel is causing concern with regard to the release of sulphur oxides. According to reports, global coal consumption has increased by 0.4% in 2014. While countries like UK, Ukraine etc. have witnessed a decline in coal consumption, some others like India, China, Africa and North America have increased their use of coal to meet the energy requirements of the growing human population. The increasing use of coal has led to extensive investigations for finding an ecofriendly clean coal technology. Dibenzothiophene (DBT) and some of its alkylated derivatives present in the form of organic sulphur in coal have received a great deal of attention in the past few years because of their recalcitrant nature. Considering the economic and environmental prospects, biodesulphurization is being regarded as an effective tool for the degradation of DBT, with concomitant application towards sulphur removal from coal. Owing to the importance of microbial applications towards production of clean coal, the present review discusses some of the recent findings in the area of DBT biodegradation. In addition, current advances in coal biodesulphurization are reviewed, concluding with a consideration of future prospects for the rapidly growing energy sector. (C) 2016 Elsevier B.V. All rights reserved.

Published
2016

15. Cyclic lipopeptide biosurfactant from Bacillus tequilensisexhibits multifarious activity

Author

Pradhan, Arun, Rath, Animesha, Pradhan, Nilotpala, Hazra, Rupenangshu, Nayak, Rati, and Kanjilal, Sanjit

Abstract

Bacillus tequilensisstrain CH had been previously shown to produce a biosurfactant. In this study, chemical structure of the purified biosurfactant was determined by using high performance liquid chromatography and liquid chromatography-mass spectroscopy as a 10 amino acid cyclic lipopeptide (CL). The cyclic lipopeptide was found to be active against Anopheles culicifacieslarvae with a LC50of 110 µg/ml in 2 days. 1 ppm cadmium (Cd) which had a profound mutagenic effect on the cell division of onion (Allium cepa) root tip cell resulting in abnormal metaphase, abnormal anaphase and nuclei elongation was partially reversed in the presence of 0.1 mg/ml of CL (52% cells dividing normally and 8% with abnormal division) and was comparable to control experiment where no Cd was present. Thus, the CL described in this report may have applications in eliminating larvae from water repository systems and in reversing the effects of cadmium pollution.

Published
2018
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17. Controlled Synthesis of Gold Nanoparticles Using Aspergillus terreus IF0 and Its Antibacterial Potential against Gram Negative Pathogenic Bacteria.

Author

Priyadarshini, Eepsita, Pradhan, Nilotpala, Sukla, Lala B., and Panda, Prasanna K.

Subjects
GOLD nanoparticle synthesis, ASPERGILLUS terreus, ANTIBACTERIAL agents, GRAM-negative bacteria, NANOBIOTECHNOLOGY, FOURIER transform infrared spectroscopy
Abstract

Biosynthesis of monodispersed nanoparticles, along with determination of potential responsible biomolecules, is the major bottleneck in the area of bionanotechnology research. The present study focuses on an ecofriendly, ambient temperature protocol for size controlled synthesis of gold nanoparticles, using the fungus Aspergillus terreus IF0. Gold nanoparticles were formed immediately, with the addition of chloroauric acid to the aqueous fungal extract. Synthesized nanoparticles were characterized by UV-Vis spectroscopy, TEM-EDX, and XRD analysis. Particle diameter and dispersity of nanoparticles were controlled by varying the pH of the fungal extract. At pH 10, the average size of the synthesized particles was in the range of 10-19 nm. Dialysis to obtain high and lowmolecular weight fraction followed by FTIR analysis revealed that biomolecules larger than 12 kDa and having -CH, - NH, and -SH functional groups were responsible for bioreduction and stabilization. In addition, the synthesized gold nanoparticles were found to be selectively bactericidal against the pathogenic gram negative bacteria, Escherichia coli. [ABSTRACT FROM AUTHOR]

Published
2014
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18. Fluorescence microscopic visualization of halophile Bacillus tequilensisCH and mixed culture of acidophilic bacteria

Author

Panda, Sandeep, Pradhan, Arun Kumar, Mishra, Srabani, and Pradhan, Nilotpala

Abstract

Halophiles have been used as bioremediation agents in degradation of organic pollutants and acidophilic microorganisms are considered as efficient metal leaching agents for mineral-metal bearing wastes. In the present study, halophilic bacteria (Bacillus tequilensisCH) and a mixed culture of acidophilic bacteria were used for their visual detection over fluorescence microscope. With regards to B. tequilensisCH, bacterial presence and its biofilm formation was studied over glass cover slips; while for the acidophilic culture, comparisons were made to observe bacterial presence over glass cover slips and a sample ore surface. It was observed that for both the microbial cultures, green fluorescence was detected at different magnifications for the bacteria as well as the biofilm formed (better visualization in case of B. tequilensis); thus, confirming the adsorption of calcein-AM dye by the live bacterial cells. Furthermore, the 3D intensity plots, in case of the acidophiles, confirmed the presence of bacterial clusters and a possible formation of bacterial biofilm over the sample ore surface.

Published
2021
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