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4 results on '"Purser, Lauren"'

1. Rare, convergent antibodies targeting the stem helix broadly neutralize diverse betacoronaviruses

Author

Dacon, Cherrelle, primary, Peng, Linghang, additional, Lin, Ting-Hui, additional, Tucker, Courtney, additional, Lee, Chang-Chun D., additional, Cong, Yu, additional, Wang, Lingshu, additional, Purser, Lauren, additional, Cooper, Andrew J.R., additional, Williams, Jazmean K., additional, Pyo, Chul-Woo, additional, Yuan, Meng, additional, Kosik, Ivan, additional, Hu, Zhe, additional, Zhao, Ming, additional, Mohan, Divya, additional, Peterson, Mary, additional, Skinner, Jeff, additional, Dixit, Saurabh, additional, Kollins, Erin, additional, Huzella, Louis, additional, Perry, Donna, additional, Byrum, Russell, additional, Lembirik, Sanae, additional, Murphy, Michael, additional, Zhang, Yi, additional, Yang, Eun Sung, additional, Chen, Man, additional, Leung, Kwanyee, additional, Weinberg, Rona S., additional, Pegu, Amarendra, additional, Geraghty, Daniel E., additional, Davidson, Edgar, additional, Doranz, Benjamin J., additional, Douagi, Iyadh, additional, Moir, Susan, additional, Yewdell, Jonathan W., additional, Schmaljohn, Connie, additional, Crompton, Peter D., additional, Mascola, John R., additional, Holbrook, Michael R., additional, Nemazee, David, additional, Wilson, Ian A., additional, and Tan, Joshua, additional

Published
2023
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2. Uptake and Retention of Nanoplastics in Quagga Mussels

Author

Merzel, Rachel L., primary, Purser, Lauren, additional, Soucy, Taylor L., additional, Olszewski, Monica, additional, Colón‐Bernal, Isabel, additional, Duhaime, Melissa, additional, Elgin, Ashley K., additional, and Banaszak Holl, Mark M., additional

Published
2019
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3. Uptake and Retention of Nanoplastics in Quagga Mussels.

Author

Merzel, Rachel L., Purser, Lauren, Soucy, Taylor L., Olszewski, Monica, Colón‐Bernal, Isabel, Duhaime, Melissa, Elgin, Ashley K., and Banaszak Holl, Mark M.

Subjects
MUSSELS, PHOTOTHERMAL spectroscopy, INFRARED spectroscopy, FRESHWATER mussels, FLUORESCENCE spectroscopy
Abstract

Here, a set of experiments to assess the feasibility of using an invasive and widespread freshwater mussel (Dreissena rostrformis bugensis) as a sentinel species for nanoplastic detection is reported. Under laboratory experimental conditions, mussels ingest and retain fluorescent polystyrene (PS) beads with carboxylic acid (COOH) termination over a size range of 200–2000 nm. The number of beads the mussels ingested is quantified using fluorescence spectroscopy and the location of the beads in the mussels is imaged using fluorescence microscopy. PS beads of similar size (1000–2000 nm) to mussels' preferred food are trafficked in the ciliated food grooves of the gills. Beads of all sizes are observed in the mussels' digestive tracts, indicating that the mussels do not efficiently reject the beads as unwanted foreign material, regardless of size. Fluorescence microscopy shows all sizes of beads are concentrated in the siphons and are retained there for longer than one month postexposure. Combined atomic force microscopy–infrared spectroscopy and photothermal infrared spectroscopy are used to locate, image, and chemically identify the beads in the mussel siphons. In sum, these experiments demonstrate the potential for using mussels, specifically their siphons, to monitor environmental accumulation of aquatic nanoplastics. [ABSTRACT FROM AUTHOR]

Published
2020
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4. Broadly neutralizing antibodies target the coronavirus fusion peptide.

Author

Dacon C, Tucker C, Peng L, Lee CD, Lin TH, Yuan M, Cong Y, Wang L, Purser L, Williams JK, Pyo CW, Kosik I, Hu Z, Zhao M, Mohan D, Cooper A, Peterson M, Skinner J, Dixit S, Kollins E, Huzella L, Perry D, Byrum R, Lembirik S, Zhang Y, Yang ES, Chen M, Leung K, Weinberg RS, Pegu A, Geraghty DE, Davidson E, Douagi I, Moir S, Yewdell JW, Schmaljohn C, Crompton PD, Holbrook MR, Nemazee D, Mascola JR, Wilson IA, and Tan J

Abstract

The potential for future coronavirus outbreaks highlights the need to develop strategies and tools to broadly target this group of pathogens. Here, using an epitope-agnostic approach, we identified six monoclonal antibodies that bound to spike proteins from all seven human-infecting coronaviruses. Epitope mapping revealed that all six antibodies target the conserved fusion peptide region adjacent to the S2' cleavage site. Two antibodies, COV44-62 and COV44-79, broadly neutralize a range of alpha and beta coronaviruses, including SARS-CoV-2 Omicron subvariants BA.1 and BA.2, albeit with lower potency than RBD-specific antibodies. In crystal structures of Fabs COV44-62 and COV44-79 with the SARS-CoV-2 fusion peptide, the fusion peptide epitope adopts a helical structure and includes the arginine at the S2' cleavage site. Importantly, COV44-79 limited disease caused by SARS-CoV-2 in a Syrian hamster model. These findings identify the fusion peptide as the target of the broadest neutralizing antibodies in an epitope-agnostic screen, highlighting this site as a candidate for next-generation coronavirus vaccine development., One-Sentence Summary: Rare monoclonal antibodies from COVID-19 convalescent individuals broadly neutralize coronaviruses by targeting the fusion peptide.

Published
2022
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