58 results on '"Ribeiro RT"'
Search Results
2. Machine learning techniques to predict the risk of developing diabetic nephropathy: a literature review
- Author
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Mesquita, F., primary, Bernardino, J., additional, Henriques, J., additional, Raposo, JF., additional, Ribeiro, RT., additional, and Paredes, S., additional
- Published
- 2023
- Full Text
- View/download PDF
3. PERFIL INICIAL DOS ATENDIMENTOS NO AMBULATÓRIO DE HEMATOLOGIA DA UNIVERSIDADE FEDERAL DE ALFENAS-MG
- Author
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Caetano, FM, primary, Silva, NB, additional, Bochi, GS, additional, Couto, DS, additional, Vieira, LS, additional, Menezes, LLS, additional, Ribeiro, RT, additional, Nogueira, LS, additional, and Rabelo, IB, additional
- Published
- 2023
- Full Text
- View/download PDF
4. LIGA DE HEMATOLOGIA DE ALFENAS: DO SONHO À CONCRETIZAÇÃO
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Silva, NB, primary, Couto, DS, additional, Menezes, LLS, additional, Bochi, GS, additional, Caetano, FM, additional, Vieira, LS, additional, Silva, ANF, additional, Ribeiro, RT, additional, Nogueira, LS, additional, and Rabelo, IB, additional
- Published
- 2022
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- View/download PDF
5. Development of an Interactive Lifestyle Programme for Adolescents at Risk of Developing Type 2 Diabetes: PRE-STARt
- Author
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Harrington, DM, Brady, EM, Weihrauch-Bluher, S, Edwardson, CL, Gray, LJ, Hadjiconstantinou, M, Jarvis, J, Khunti, K, Vergara, I, Erreguerena, I, Ribeiro, RT, Troughton, J, Vazeou, A, Davies, MJ, Harrington, DM, Brady, EM, Weihrauch-Bluher, S, Edwardson, CL, Gray, LJ, Hadjiconstantinou, M, Jarvis, J, Khunti, K, Vergara, I, Erreguerena, I, Ribeiro, RT, Troughton, J, Vazeou, A, and Davies, MJ
- Abstract
BACKGROUND: Type 2 diabetes (T2D) is increasing in young people. Reporting on the processes used when developing prevention interventions is needed. We present the development of a family-based interactive lifestyle intervention for adolescents with risk factors for T2D in the future. METHOD: A multidisciplinary team in the UK site led the intervention development process with sites in Portugal, Greece, Germany and Spain. Potential programme topics and underpinning theory were gathered from literature and stakeholders. A theoretical framework based on self-efficacy theory and the COM-B (capability, opportunity, motivation, behaviour) model was developed. Sessions and supporting resources were developed and refined via two iterative cycles of session and resource piloting, feedback, reflection and refinement. Decision on delivery and content were made by stakeholders (young people, teachers, parents, paediatricians) and all sites. Materials were translated to local languages. Site-specific adaptations to the language, content and supporting resources were made. RESULTS: The "PRE-STARt" programme is eight 90-min interactive sessions with supporting curriculum and resources. Iterative development work provided valuable feedback on programme content and delivery. CONCLUSION: Reporting on the intervention development process, which includes stakeholder input, could yield a flexible approach for use in this emerging 'at risk' groups and their families.
- Published
- 2021
6. FOCAL ADHESION KINASE INHIBITION DECREASES CELL VIABILITY AND INDUCES APOPTOSIS IN SET-2 CELLS
- Author
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Valente, ACMM, Pereira, MBM, and Ribeiro, RT
- Published
- 2022
- Full Text
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7. EHMTI-0267. Plasma anandamide concentration after aerobic exercise training in healthy individuals and episodic migraine patients
- Author
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Oliveira, AB, Ribeiro, RT, Mello, MT, Tufik, S, and Peres, MFP
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- 2014
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8. EHMTI-0200. Aerobic exercise training at the ventilatory threshold prevents migraine and improves mood
- Author
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Oliveira, AB, Ribeiro, RT, Mello, MT, Tufik, S, and Peres, MFP
- Published
- 2014
- Full Text
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9. Una oportunitat per intercanviar bones pràctiques en l’atenció a la cronicitat a nivell europeu: el projecte JA-CHRODIS
- Author
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Robles, Noemí, Giménez, Emmanuel, Carrion-Ribas, Carme, Ribeiro, Rogério T., Reynolds, Jillian, Barbaglia, María G., Domingo-Torrell, Laia, Massip-Salcedo, Marta, Serra-Sutton, Vicky, Espallargues-Carreras, Mireia, [Robles N] Universitat Autònoma de Barcelona (UAB), Bellaterra, Cerdanyola del Vallès, Spain. Red de Investigación en Servicios de Salud en Enfermedades Crónicas (REDISSEC), Barcelona, Spain. [Giménez E, Reynolds J] Agència de Qualitat i Avaluació Sanitàries de Catalunya (AQuAS), Departament de Salut, Generalitat de Catalunya, Barcelona, Spain. [Carrion C] Red de Investigación en Servicios de Salud en Enfermedades Crónicas (REDISSEC), Barcelona, Spain. Estudis de Ciències de la Salut, Universitat Oberta de Catalunya (UOC), Barcelona, Spain. [Ribeiro RT] Associação Protectora dos Diabéticos de Portugal (APDP), Lisboa, Portugal. [Barbaglia MG] Red de Investigación en Servicios de Salud en Enfermedades Crónicas (REDISSEC), Barcelona, Spain. Agència de Salut Pública de Barcelona (ASPB), Ajuntament de Barcelona, Barcelona, Spain. [Domingo L] Red de Investigación en Servicios de Salud en Enfermedades Crónicas (REDISSEC), Barcelona, Spain. Institut Hospital del Mar d’Investigacions Mèdiques (IMIM), Barcelona, Spain. [Massip-Salcedo M] Estudis de Ciències de la Salut, Universitat Oberta de Catalunya (UOC), Barcelona, Spain. [Serra-Sutton V] Agència de Qualitat i Avaluació Sanitàries de Catalunya (AQuAS), Departament de Salut, Generalitat de Catalunya, Barcelona, Spain. CIBER de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain. [Espallargues M] Red de Investigación en Servicios de Salud en Enfermedades Crónicas (REDISSEC), Barcelona, Spain. Agència de Qualitat i Avaluació Sanitàries de Catalunya (AQuAS), Departament de Salut, Generalitat de Catalunya, Barcelona, Spain, and Departament de Salut
- Subjects
Unión Europea ,Promoció de la salut - Europa ,afecciones patológicas, signos y síntomas::procesos patológicos::atributos de la enfermedad::enfermedad crónica [ENFERMEDADES] ,Pathological Conditions, Signs and Symptoms::Pathologic Processes::Disease Attributes::Chronic Disease [DISEASES] ,Malalties cròniques - Prevenció ,European Union ,Atención a la Salud::Promoción de la Salud [SALUD PÚBLICA] ,Malalts crònics - Assistència mèdica ,Health Care (Public Health)::Health Promotion [PUBLIC HEALTH] - Abstract
Good practices; Chronic patient; JA-CHRODIS Buenas prácticas; Paciente crónico; JA-CHRODIS Bones pràctiques; Pacient crònic; JA-CHRODIS El nombre de persones que pateixen malalties cròniques ha augmentat considerablement durant les últimes dècades. Aquesta situación ha provocat que el sistema sanitari hagi hagut de reorientar-se i desenvolupar diverses pràctiques que pretenen optimitzar l’atenció als pacients crònics. La Comissió Europea va posar en marxa el 2014 una acció conjunta en la qual han participat 60 institucions que pertanyen a 26 països de la Unió Europea; el projecte JA-CHRODIS. L’objectiu de l’acció ha consistit en identificar les millors experiències que s’estan duent a terme actualment a Europa en atenció a la cronicitat, a partir d’uns criteris d’avaluació unificats i consensuats, per tal de posar-les a disposició tant dels professionals com dels gestors i responsables polítics de serveis d’atenció a la salut a través d’un repositori; es permet així l’intercanvi d’informació sobre aquelles experiències més exitoses i es promou la transferència i la implementació de les millors pràctiques. El número de personas que sufren enfermedades crónicas ha aumentado considerablemente durante las últimas décadas. Esta situación ha provocado que el sistema sanitario haya tenido que reorientarse y desarrollar varias prácticas que pretenden optimizar la atención a los pacientes crónicos. La Comisión Europea puso en marcha el 2014 una acción conjunta en la cual han participado 60 instituciones que pertenecen en 26 países de la Unión Europea; el proyecto JA-CHRODIS. El objetivo de la acción ha consistido en identificar las mejores experiencias que se están llevando a cabo actualmente en Europa en atención a la cronicidad, a partir de unos criterios de evaluación unificados y consensuados, para ponerlas a disposición tanto de los profesionales cómo de los gestores y responsables políticos de servicios de atención a la salud a través de un repositorio; se permite así el intercambio de información sobre aquellas experiencias más exitosas y se promueve la transferencia y la implementación de las mejores prácticas. The number of people are living with a chronic disease has increased considerably during the last decades. This situation has caused a change in the health system. It has to reorient itself and develop several practices that aim to optimize the care of chronic patients. The European Commission launched in 2014 a joint action in which 60 institutions that belong in 26 countries of the European Union have participated; the JA-CHRODIS project. The objective of the action has been to identify the best experiences that is being currently carried out in Europe about attention to chronicity, based on unified and agreed evaluation criteria, to make them available to both professionals and managers and policy makers of health care services through a repository; In this way allows the exchange of information on those most successful experiences and promotes the transfer and implementation of best practices.
10. The Ex-Timing trial: evaluating morning, afternoon, and evening exercise on the circadian clock in individuals with type 2 diabetes and overweight/obesity-a randomized crossover study protocol.
- Author
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Magalhães JP, Oliveira EC, Hetherington-Rauth M, Jesus F, Rodrigues MC, Raposo JF, Ribeiro RT, Caetano C, and Sardinha LB
- Subjects
- Humans, Time Factors, Circadian Clocks, Middle Aged, Male, Female, Overweight therapy, Overweight physiopathology, Exercise Therapy methods, Treatment Outcome, Aged, Glycemic Control methods, Exercise, Diabetes Mellitus, Type 2 blood, Diabetes Mellitus, Type 2 therapy, Diabetes Mellitus, Type 2 physiopathology, Cross-Over Studies, Obesity therapy, Obesity physiopathology, Obesity blood, Blood Glucose metabolism, Randomized Controlled Trials as Topic, High-Intensity Interval Training methods
- Abstract
Background: Exercise is known to provide multiple metabolic benefits such as improved insulin sensitivity and glucose control in individuals with type 2 diabetes mellitus (T2DM) and those at risk. Beyond the traditional exercise dose, exercise timing is perceived as a contemporary hot topic, especially in the field of T2DM; however, the number of intervention studies assessing exercise timing and glucose metabolism is scarce. Our aim is to test the effect of exercise timing (i.e., morning, afternoon, or evening) on the inter-individual response variability in glycemic control and related metabolic health parameters in individuals with T2DM and those at risk during a 12-week intervention., Methods: A randomized crossover exercise intervention will be conducted involving two groups: group 1, individuals with T2DM; group 2, age-matched older adults with overweight/obesity. The intervention will consist of three 2-week blocks of supervised post-prandial exercise using high-intensity interval training (HIIT). Between each training block, a 2-week washout period, where participants avoid structured exercise, will take place. Assessments will be conducted in both groups before and after each exercise block. The primary outcomes include the 24-h area under the curve continuous glucose monitoring-based glucose. The secondary outcomes include body composition, resting energy expenditure, insulin response to a meal tolerance test, maximal aerobic capacity, peak power output, physical activity, sleep quality, and insulin and glucose levels. All primary and secondary outcomes will be measured at each assessment point., Discussion: Outcomes from this trial will provide us additional insight into the role of exercise timing on the inter-individual response variability in glycemic control and other related metabolic parameters in two distinct populations, thus contributing to the development of more effective exercise prescription guidelines for individuals with T2DM and those at risk., Trial Registration: ClinicalTrials.gov NCT06136013. Registered on November 18, 2023., (© 2024. The Author(s).)
- Published
- 2024
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11. 3-Hydroxy-3-Methylglutaric Acid Disrupts Brain Bioenergetics, Redox Homeostasis, and Mitochondrial Dynamics and Affects Neurodevelopment in Neonatal Wistar Rats.
- Author
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Silveira JA, Marcuzzo MB, da Rosa JS, Kist NS, Hoffmann CIH, Carvalho AS, Ribeiro RT, Quincozes-Santos A, Netto CA, Wajner M, and Leipnitz G
- Abstract
3-Hydroxy-3-methylglutaric acidemia (HMGA) is a neurometabolic inherited disorder characterized by the predominant accumulation of 3-hydroxy-3-methylglutaric acid (HMG) in the brain and biological fluids of patients. Symptoms often appear in the first year of life and include mainly neurological manifestations. The neuropathophysiology is not fully elucidated, so we investigated the effects of intracerebroventricular administration of HMG on redox and bioenergetic homeostasis in the cerebral cortex and striatum of neonatal rats. Neurodevelopment parameters were also evaluated. HMG decreased the activity of glutathione reductase (GR) and increased catalase (CAT) in the cerebral cortex. In the striatum, HMG reduced the activities of superoxide dismutase, glutathione peroxidase, CAT, GR, glutathione S-transferase, and glucose-6-phosphate dehydrogenase. Regarding bioenergetics, HMG decreased the activities of succinate dehydrogenase and respiratory chain complexes II-III and IV in the cortex. HMG also decreased the activities of citrate synthase and succinate dehydrogenase, as well as complex IV in the striatum. HMG further increased DRP1 levels in the cortex, indicating mitochondrial fission. Finally, we found that the HMG-injected animals showed impaired performance in all sensorimotor tests examined. Our findings provide evidence that HMG causes oxidative stress, bioenergetic dysfunction, and neurodevelopmental changes in neonatal rats, which may explain the neuropathophysiology of HMGA.
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- 2024
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12. Sulfite Impairs Bioenergetics and Redox Status in Neonatal Rat Brain: Insights into the Early Neuropathophysiology of Isolated Sulfite Oxidase and Molybdenum Cofactor Deficiencies.
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Pramio J, Grings M, da Rosa AG, Ribeiro RT, Glanzel NM, Signori MF, Marcuzzo MB, Bobermin LD, Wyse ATS, Quincozes-Santos A, Wajner M, and Leipnitz G
- Subjects
- Animals, Rats, Animals, Newborn, Oxidation-Reduction, Rats, Wistar, Homeostasis, Mitochondria metabolism, Antioxidants metabolism, Energy Metabolism, Sulfites adverse effects, Sulfite Oxidase metabolism, Molybdenum Cofactors metabolism, Cerebral Cortex drug effects, Cerebral Cortex metabolism
- Abstract
Isolated sulfite oxidase (ISOD) and molybdenum cofactor (MoCD) deficiencies are genetic diseases biochemically characterized by the toxic accumulation of sulfite in the tissues of patients, including the brain. Neurological dysfunction and brain abnormalities are commonly observed soon after birth, and some patients also have neuropathological alterations in the prenatal period (in utero). Thus, we investigated the effects of sulfite on redox and mitochondrial homeostasis, as well as signaling proteins in the cerebral cortex of rat pups. One-day-old Wistar rats received an intracerebroventricular administration of sulfite (0.5 µmol/g) or vehicle and were euthanized 30 min after injection. Sulfite administration decreased glutathione levels and glutathione S-transferase activity, and increased heme oxygenase-1 content in vivo in the cerebral cortex. Sulfite also reduced the activities of succinate dehydrogenase, creatine kinase, and respiratory chain complexes II and II-III. Furthermore, sulfite increased the cortical content of ERK1/2 and p38. These findings suggest that redox imbalance and bioenergetic impairment induced by sulfite in the brain are pathomechanisms that may contribute to the neuropathology of newborns with ISOD and MoCD. Sulfite disturbs antioxidant defenses, bioenergetics, and signaling pathways in the cerebral cortex of neonatal rats. CII: complex II; CII-III: complex II-III; CK: creatine kinase; GST: glutathione S-transferase; HO-1: heme oxygenase-1; SDH: succinate dehydrogenase; SO
3 2- : sulfite., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)- Published
- 2023
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13. Transient Coatings from Nanoparticles Achieving Broad-Spectrum and High Antimicrobial Performance.
- Author
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Zaia R, Quinto GM, Camargo LCS, Ribeiro RT, and Carmona-Ribeiro AM
- Abstract
Cationic and hydrophilic coatings based on casting and drying water dispersions of two different nanoparticles (NPs) onto glass are here described and evaluated for antimicrobial activity. Discoid cationic bilayer fragments (BF) surrounded by carboxy-methylcellulose (CMC) and poly (diallyl dimethyl ammonium) chloride (PDDA) NPs and spherical gramicidin D (Gr) NPs dispersed in water solution were cast onto glass coverslips and dried, forming a coating quantitatively evaluated against Pseudomonas aeruginosa , Staphylococcus aureus and Candida albicans . From plating and colony forming units (CFU) counting, all strains interacting for 1 h with the coatings lost viability from 10
5 to 106 , to zero CFU, at two sets of Gr and PDDA doses: 4.6 and 25 μg, respectively, or, 0.94 and 5 μg, respectively. Combinations produced broad spectrum, antimicrobial coatings; PDDA electrostatically attached to the microbes damaging cell walls, allowing Gr NPs interaction with the cell membrane. This concerted action promoted optimal activity at low Gr and PDDA doses. Further washing and drying of the deposited dried coatings showed that they were washed out so that antimicrobial activity was no longer present on the glass surface. Significant applications in biomedical materials can be foreseen for these transient coatings.- Published
- 2023
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14. Disruption of mitochondrial bioenergetics and calcium homeostasis by phytanic acid in the heart: Potential relevance for the cardiomyopathy in Refsum disease.
- Author
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Zemniaçak ÂB, Roginski AC, Ribeiro RT, Bender JG, Marschner RA, Wajner SM, Wajner M, and Amaral AU
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- Rats, Animals, Phytanic Acid pharmacology, Phytanic Acid metabolism, Calcium metabolism, Rats, Wistar, Energy Metabolism, Mitochondria, Heart metabolism, Fatty Acids metabolism, Mitochondrial Permeability Transition Pore metabolism, Homeostasis, Refsum Disease metabolism, Cardiomyopathies drug therapy, Cardiomyopathies metabolism
- Abstract
Refsum disease is an inherited peroxisomal disorder caused by severe deficiency of phytanoyl-CoA hydroxylase activity. Affected patients develop severe cardiomyopathy of poorly known pathogenesis that may lead to a fatal outcome. Since phytanic acid (Phyt) concentrations are highly increased in tissues of individuals with this disease, it is conceivable that this branched-chain fatty acid is cardiotoxic. The present study investigated whether Phyt (10-30 μM) could disturb important mitochondrial functions in rat heart mitochondria. We also determined the influence of Phyt (50-100 μM) on cell viability (MTT reduction) in cardiac cells (H9C2). Phyt markedly increased mitochondrial state 4 (resting) and decreased state 3 (ADP-stimulated) and uncoupled (CCCP-stimulated) respirations, besides reducing the respiratory control ratio, ATP synthesis and the activities of the respiratory chain complexes I-III, II, and II-III. This fatty acid also reduced mitochondrial membrane potential and induced swelling in mitochondria supplemented by exogenous Ca
2+ , which were prevented by cyclosporin A alone or combined with ADP, suggesting the involvement of the mitochondrial permeability transition (MPT) pore opening. Mitochondrial NAD(P)H content and Ca2+ retention capacity were also decreased by Phyt in the presence of Ca2+ . Finally, Phyt significantly reduced cellular viability (MTT reduction) in cultured cardiomyocytes. The present data indicate that Phyt, at concentrations found in the plasma of patients with Refsum disease, disrupts by multiple mechanisms mitochondrial bioenergetics and Ca2+ homeostasis, which could presumably be involved in the cardiomyopathy of this disease., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
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15. Uncovering Actions of Type 3 Deiodinase in the Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD).
- Author
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Marschner RA, Roginski AC, Ribeiro RT, Longo L, Álvares-da-Silva MR, and Wajner SM
- Subjects
- Rats, Animals, Male, Rats, Sprague-Dawley, Thyroid Hormones metabolism, Triiodothyronine metabolism, Iodide Peroxidase metabolism, Non-alcoholic Fatty Liver Disease
- Abstract
Metabolic dysfunction-associated fatty liver disease (MAFLD) has gained worldwide attention as a public health problem. Nonetheless, lack of enough mechanistic knowledge restrains effective treatments. It is known that thyroid hormone triiodothyronine (T3) regulates hepatic lipid metabolism, and mitochondrial function. Liver dysfunction of type 3 deiodinase (D3) contributes to MAFLD, but its role is not fully understood., Objective: To evaluate the role of D3 in the progression of MAFLD in an animal model., Methodology: Male/adult Sprague Dawley rats (n = 20) were allocated to a control group (2.93 kcal/g) and high-fat diet group (4.3 kcal/g). Euthanasia took place on the 28th week. D3 activity and expression, Uncoupling Protein 2 (UCP2) and type 1 deiodinase (D1) expression, oxidative stress status, mitochondrial, Krebs cycle and endoplasmic reticulum homeostasis in liver tissue were measured., Results: We observed an increase in D3 activity/expression ( p < 0.001) related to increased thiobarbituric acid reactive substances (TBARS) and carbonyls and diminished reduced glutathione (GSH) in the MAFLD group ( p < 0.05). There was a D3-dependent decrease in UCP2 expression ( p = 0.01), mitochondrial capacity, respiratory activity with increased endoplasmic reticulum stress in the MAFLD group ( p < 0.001). Surprisingly, in an environment with lower T3 levels due to high D3 activity, we observed an augmented alpha-ketoglutarate dehydrogenase (KGDH) and glutamate dehydrogenase (GDH) enzymes activity ( p < 0.05)., Conclusion: Induced D3, triggered by changes in the REDOX state, decreases T3 availability and hepatic mitochondrial capacity. The Krebs cycle enzymes were altered as well as endoplasmic reticulum stress. Taken together, these results shed new light on the role of D3 metabolism in MAFLD.
- Published
- 2023
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16. Sensitive Zika biomarker detection assisted by quantum dot-modified electrochemical immunosensing platform.
- Author
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Ribeiro JFF, Melo JRS, Santos CL, Chaves CR, Cabral Filho PE, Pereira G, Santos BS, Pereira GAL, Rosa DS, Ribeiro RT, and Fontes A
- Subjects
- Humans, Tellurium chemistry, Biomarkers metabolism, Quantum Dots chemistry, Zika Virus metabolism, Zika Virus Infection diagnosis, Cadmium Compounds chemistry, Biosensing Techniques methods
- Abstract
We report the development of a new nanostructured electrochemical immunosensing platform for the detection of the Zika virus envelope protein (EP-ZIKV). For this, quantum dots (QDs) were explored in combination with screen-printed carbon electrodes (SPCEs) functionalized with a conductor polymeric film, formed from 2-(1H-pyrrol-1-yl)ethanamine (Py
am ), and anti-EP DIII ZIKV antibodies. Carboxylated CdTe QDs were synthesized, characterized by optical and structural techniques, and covalently immobilized onto the SPCE/PPyam surface. Then, anti-EP ZIKV antibodies were also covalently conjugated to QDs. All stages of platform assembly were evaluated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The detection of EP-ZIKV was performed by differential pulse voltammetry (DPV). Results indicated that QDs were efficiently immobilized, and did not show oxidation, under the conditions evaluated, for at least 7 months. Anti-EP ZIKV antibodies were effectively immobilized on the PPyam /QDs surface, even after 2 months of electrode storage. The platform enabled the detection of EP-ZIKV with high sensitivity using minimal sample volumes (LOD = 0.1 ng mL-1 and LOQ = 0.4 ng mL-1 ). The platform was also able to detect EP-ZIKV in spiked serum samples. Moreover, the platform showed specificity, not detecting the EP-DENV 3 nor a mixture of four DENV serotypes antigens. Thus, the proposed combination favored the development of a sensitive immunosensing platform, promising for the detection of Zika in the viremic phase, which also holds potential for transposition to other arboviruses., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)- Published
- 2023
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17. Increased Intake of Both Caffeine and Non-Caffeine Coffee Components Is Associated with Reduced NAFLD Severity in Subjects with Type 2 Diabetes.
- Author
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Coelho M, Patarrão RS, Sousa-Lima I, Ribeiro RT, Meneses MJ, Andrade R, Mendes VM, Manadas B, Raposo JF, Macedo MP, and Jones JG
- Subjects
- Humans, Coffee, Caffeine, Theophylline, Chromatography, Liquid, Overweight complications, Tandem Mass Spectrometry, Liver Cirrhosis complications, Surveys and Questionnaires, Obesity complications, Non-alcoholic Fatty Liver Disease complications, Diabetes Mellitus, Type 2 complications
- Abstract
Coffee may protect against non-alcoholic fatty liver disease (NAFLD), but the roles of the caffeine and non-caffeine components are unclear. Coffee intake by 156 overweight subjects (87% with Type-2-Diabetes, T2D) was assessed via a questionnaire, with 98 subjects (all T2D) also providing a 24 h urine sample for quantification of coffee metabolites by LC-MS/MS. NAFLD was characterized by the fatty liver index (FLI) and by Fibroscan
® assessment of fibrosis. No associations were found between self-reported coffee intake and NAFLD parameters; however, total urine caffeine metabolites, defined as Σcaffeine (caffeine + paraxanthine + theophylline), and adjusted for fat-free body mass, were significantly higher for subjects with no liver fibrosis than for those with fibrosis. Total non-caffeine metabolites, defined as Σncm (trigonelline + caffeic acid + p -coumaric acid), showed a significant negative association with the FLI. Multiple regression analyses for overweight/obese T2D subjects (n = 89) showed that both Σcaffeine and Σncm were negatively associated with the FLI, after adjusting for age, sex, HbA1c , ethanol intake and glomerular filtration rate. The theophylline fraction of Σcaffeine was significantly increased with both fibrosis and the FLI, possibly reflecting elevated CYP2E1 activity-a hallmark of NAFLD worsening. Thus, for overweight/obese T2D patients, higher intake of both caffeine and non-caffeine coffee components is associated with less severe NAFLD. Caffeine metabolites represent novel markers of NAFLD progression.- Published
- 2022
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18. Interpersonal Relationships in Diabetes: Views and Experience of People with Diabetes, Informal Carers, and Healthcare Professionals in Portugal.
- Author
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Nascimento do Ó D, Serrabulho L, Ribeiro RT, Silva S, Covinhas A, Afonso MJ, Boavida JM, and Raposo JF
- Subjects
- Adult, Humans, Portugal, Caregivers, Self Care psychology, Delivery of Health Care, Quality of Life, Diabetes Mellitus therapy
- Abstract
Introduction: The increasing burden of diabetes poses a great challenge to healthcare systems and economy worldwide. Although modern therapeutic strategies for diabetes are widely available, most patients still fail to achieve optimal clinical targets and well-being. The primary objective of this study was to assess and explore potential drivers and successful management of diabetes among people with diabetes, family members and healthcare professionals in Portugal, by applying the protocol of the multinational study "Diabetes, Attitudes, Wishes and Needs (DAWN2)"., Material and Methods: A total of 767 adults, including 417 people with diabetes, 123 family members and 227 healthcare professionals, participated in the study. Surveys assessed health-related quality of life, self-management, attitudes/beliefs, social support and priorities for improvement areas in diabetes care., Results: Diabetes has a negative impact on the physical health and emotional well-being of patients in Portugal and is also a psychological burden for family members. Earlier diagnosis and treatment of diabetes were mentioned as a major area of improvement. Healthcare professionals indicated the need for diabetes self-management education., Conclusion: We have used for the first time in Portugal the DAWN2 protocol to address the wishes, needs, and attitudes of Portuguese diabetes patients, their relatives, and healthcare professionals regarding the disease.
- Published
- 2022
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19. Characterization and Differential Cytotoxicity of Gramicidin Nanoparticles Combined with Cationic Polymer or Lipid Bilayer.
- Author
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Pérez-Betancourt Y, Zaia R, Evangelista MF, Ribeiro RT, Roncoleta BM, Mathiazzi BI, and Carmona-Ribeiro AM
- Abstract
Gramicidin (Gr) nanoparticles (NPs) and poly (diallyl dimethyl ammonium) chloride (PDDA) water dispersions were characterized and evaluated against Gram-positive and Gram-negative bacteria and fungus. Dynamic light scattering for sizing, zeta potential analysis, polydispersity, and colloidal stability over time characterized Gr NPs/PDDA dispersions, and plating and colony-forming units counting determined their microbicidal activity. Cell viabilities of Staphylococcus aureus , Pseudomonas aeruginosa , and Candida albicans in the presence of the combinations were reduced by 6, 7, and 7 logs, respectively, at 10 μM Gr/10 μg·mL
-1 PDDA, 0.5 μM Gr/0. 5μg·mL-1 PDDA, and 0.5 μM Gr/0.5 μg·mL-1 PDDA, respectively. In comparison to individual Gr doses, the combinations reduced doses by half ( S. aureus ) and a quarter ( C. albicans ); in comparison to individual PDDA doses, the combinations reduced doses by 6 times ( P. aeruginosa ) and 10 times ( C. albicans ). Gr in supported or free cationic lipid bilayers reduced Gr activity against S. aureus due to reduced Gr access to the pathogen. Facile Gr NPs/PDDA disassembly favored access of each agent to the pathogen: PDDA suctioned the pathogen cell wall facilitating Gr insertion in the pathogen cell membrane. Gr NPs/PDDA differential cytotoxicity suggested the possibility of novel systemic uses for the combination.- Published
- 2022
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20. Influence of Altered Thyroid Hormone Mechanisms in the Progression of Metabolic Dysfunction Associated with Fatty Liver Disease (MAFLD): A Systematic Review.
- Author
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Marschner RA, Arenhardt F, Ribeiro RT, and Wajner SM
- Abstract
We performed a systematic review of the mechanisms of thyroid hormones (THs) associated with metabolic dysfunction associated with fatty liver disease (MAFLD). This systematic review was registered under PROSPERO (CRD42022323766). We searched the MEDLINE (via PubMed) and Embase databases from their inception to March 2022. We included studies that assessed thyroid function by measuring the serum level of THs and those involved in MAFLD. We excluded reviews, case reports, editorials, letters, duplicate studies and designed controls. Forty-three studies included MAFLD, eleven analyzed THs, and thirty-two evaluated the mechanisms of THs in MAFLD. Thyroid hormones are essential for healthy growth, development and tissue maintenance. In the liver, THs directly influence the regulation of lipid and carbohydrate metabolism, restoring the homeostatic state of the body. The selected studies showed an association of reduced levels of THs with the development and progression of MAFLD. In parallel, reduced levels of T3 have a negative impact on the activation of co-regulators in the liver, reducing the transcription of genes important in hepatic metabolism. Overall, this is the first review that systematically synthesizes studies focused on the mechanism of THs in the development and progression of MAFLD. The data generated in this systematic review strengthen knowledge of the impact of TH changes on the liver and direct new studies focusing on therapies that use these mechanisms.
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- 2022
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21. Prediabetes blunts DPP4 genetic control of postprandial glycaemia and insulin secretion.
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Patarrão RS, Duarte N, Coelho I, Ward J, Ribeiro RT, Meneses MJ, Andrade R, Costa J, Correia I, Boavida JM, Duarte R, Gardete-Correia L, Medina JL, Pell J, Petrie J, Raposo JF, Macedo MP, and Penha-Gonçalves C
- Subjects
- Animals, Blood Glucose metabolism, C-Peptide metabolism, Dipeptidyl Peptidase 4 metabolism, Glucose Tolerance Test, Humans, Insulin metabolism, Insulin Secretion genetics, Mice, Diabetes Mellitus, Type 2 metabolism, Prediabetic State metabolism
- Abstract
Aims/hypothesis: Imbalances in glucose metabolism are hallmarks of clinically silent prediabetes (defined as impaired fasting glucose and/or impaired glucose tolerance) representing dysmetabolism trajectories leading to type 2 diabetes. CD26/dipeptidyl peptidase 4 (DPP4) is a clinically proven molecular target of diabetes-controlling drugs but the DPP4 gene control of dysglycaemia is not proven., Methods: We dissected the genetic control of post-OGTT and insulin release responses by the DPP4 gene in a Portuguese population-based cohort of mainly European ancestry that comprised individuals with normoglycaemia and prediabetes, and in mouse experimental models of Dpp4 deficiency and hyperenergetic diet., Results: In individuals with normoglycaemia, DPP4 single-nucleotide variants governed glycaemic excursions (rs4664446, p=1.63x10
-7 ) and C-peptide release responses (rs2300757, p=6.86x10-5 ) upon OGTT. Association with blood glucose levels was stronger at 30 min OGTT, but a higher association with the genetic control of insulin secretion was detected in later phases of the post-OGTT response, suggesting that the DPP4 gene directly senses glucose challenges. Accordingly, in mice fed a normal chow diet but not a high-fat diet, we found that, under OGTT, expression of Dpp4 is strongly downregulated at 30 min in the mouse liver. Strikingly, no genetic association was found in prediabetic individuals, indicating that post-OGTT control by DPP4 is abrogated in prediabetes. Furthermore, Dpp4 KO mice provided concordant evidence that Dpp4 modulates post-OGTT C-peptide release in normoglycaemic but not dysmetabolic states., Conclusions/interpretation: These results showed the DPP4 gene as a strong determinant of post-OGTT levels via glucose-sensing mechanisms that are abrogated in prediabetes. We propose that impairments in DPP4 control of post-OGTT insulin responses are part of molecular mechanisms underlying early metabolic disturbances associated with type 2 diabetes., (© 2021. The Author(s).)- Published
- 2022
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22. Plinia trunciflora Extract Administration Prevents HI-Induced Oxidative Stress, Inflammatory Response, Behavioral Impairments, and Tissue Damage in Rats.
- Author
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Carvalho AVS, Ribeiro RT, Durán-Carabali LE, Martini APR, Hoeper E, Sanches EF, Konrath EL, Dalmaz C, Wajner M, and Netto CA
- Subjects
- Animals, Animals, Newborn, Behavior, Animal drug effects, Brain drug effects, Brain pathology, Brain physiopathology, Fruit chemistry, Glutathione Peroxidase metabolism, Hypoxia-Ischemia, Brain complications, Hypoxia-Ischemia, Brain physiopathology, Lipid Peroxidation drug effects, Male, Neurons pathology, Oxidation-Reduction drug effects, Oxidative Stress drug effects, Rats, Rats, Wistar, Hypoxia-Ischemia, Brain drug therapy, Myrtaceae chemistry, Neuroinflammatory Diseases prevention & control, Neuroprotective Agents, Plant Extracts administration & dosage
- Abstract
The disruption of redox homeostasis and neuroinflammation are key mechanisms in the pathogenesis of brain hypoxia-ischemia (HI); medicinal plants have been studied as a therapeutic strategy, generally associated with the prevention of oxidative stress and inflammatory response. This study evaluates the neuroprotective role of the Plinia trunciflora fruit extract (PTE) in neonatal rats submitted to experimental HI. The HI insult provoked a marked increase in the lipoperoxidation levels and glutathione peroxidase (GPx) activity, accompanied by a decrease in the brain concentration of glutathione (GSH). Interestingly, PTE was able to prevent most of the HI-induced pro-oxidant effects. It was also observed that HI increased the levels of interleukin-1β in the hippocampus, and that PTE-treatment prevented this effect. Furthermore, PTE was able to prevent neuronal loss and astrocyte reactivity induced by HI, as demonstrated by NeuN and GFAP staining, respectively. PTE also attenuated the anxiety-like behavior and prevented the spatial memory impairment caused by HI. Finally, PTE prevented neural tissue loss in the brain hemisphere, the hippocampus, cerebral cortex, and the striatum ipsilateral to the HI. Taken together our results provide good evidence that the PTE extract has the potential to be investigated as an adjunctive therapy in the treatment of brain insult caused by neonatal hypoxia-ischemia.
- Published
- 2022
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23. Hypoplasia of C1's posterior arch: Is there an ideal anatomical classification?
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Junior MGP, Dos Santos NADSQ, Ribeiro RT, Landeiro JA, and Pessoa BL
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Background: Congenital anomalies of the atlas are rare and usually occur in conjunction with other congenital variants. They include a wide spectrum of anomalies ranging from clefts to hypoplasia or aplasia of its arches that may contribute to spinal cord compressive syndrome., Case Description: A 54-year-old male presented with the sudden onset of a severe quadriparesis and loss of proprioception after a minor fall. The magnetic resonance (MR) scan showed cord compression at the C1 level attributed to C1 arch hypoplasia. Two months following a decompressive C1 laminectomy without fusion, and the patient was symptom free., Conclusion: Posterior C1 arch hypoplasia is a rare anomaly that can contribute to cervical cord compression and myelopathy. The optimal surgical management may include, as in this case, a posterior decompression without fusion., Competing Interests: There are no conflicts of interest., (Copyright: © 2021 Surgical Neurology International.)
- Published
- 2021
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24. Effects of Meal Fructose/Glucose Composition on Postprandial Glucose Appearance and Hepatic Glycogen Synthesis in Healthy Subjects.
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Barosa C, Ribeiro RT, Andrade R, Raposo JF, and Jones JG
- Abstract
Dietary fructose overshadows glucose in promoting metabolic complications. Intestinal fructose metabolism (IFM) protects against these effects in rodents, by favoring gluconeogenesis, but the extent of IFM in humans is not known. We therefore aimed to infer the extent of IFM by comparing the contribution of dietary fructose to systemic glucose and hepatic glycogen appearance postprandially. Twelve fasting healthy subjects ingested two protein meals in random order, one supplemented with 50 g 5/95 fructose/glucose (LF) and the other with 50 g 55/45 fructose/glucose (HF). Sources of postprandial plasma glucose appearance and hepatic glycogen synthesis were determined with deuterated water. Plasma glucose excursions, as well as pre- and post-meal insulin, c-peptide, and triglyceride levels were nearly identical for both meals. The total gluconeogenic contribution to plasma glucose appearance was significantly higher for HF versus LF (65 ± 2% vs. 34 ± 3%, p < 0.001). For HF, Krebs cycle anaplerosis accounted for two-thirds of total gluconeogenesis (43 ± 2%) with one-third from Triose-P sources (22 ± 1%). With LF, three-quarters of the total gluconeogenic contribution originated via Krebs cycle anaplerosis (26 ± 2%) with one-quarter from Triose-P sources (9 ± 2%). HF and LF gave similar direct and indirect pathway contributions to hepatic glycogen synthesis. Increasing the fructose/glucose ratio had significant effects on glucose appearance sources but no effects on hepatic glycogen synthesis sources, consistent with extensive IFM. The majority of fructose carbons were converted to glucose via the Krebs cycle.
- Published
- 2021
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25. Development of an Interactive Lifestyle Programme for Adolescents at Risk of Developing Type 2 Diabetes: PRE-STARt.
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Harrington DM, Brady EM, Weihrauch-Bluher S, Edwardson CL, Gray LJ, Hadjiconstantinou M, Jarvis J, Khunti K, Vergara I, Erreguerena I, Ribeiro RT, Troughton J, Vazeou A, and Davies MJ
- Abstract
Background: Type 2 diabetes (T2D) is increasing in young people. Reporting on the processes used when developing prevention interventions is needed. We present the development of a family-based interactive lifestyle intervention for adolescents with risk factors for T2D in the future., Method: A multidisciplinary team in the UK site led the intervention development process with sites in Portugal, Greece, Germany and Spain. Potential programme topics and underpinning theory were gathered from literature and stakeholders. A theoretical framework based on self-efficacy theory and the COM-B (capability, opportunity, motivation, behaviour) model was developed. Sessions and supporting resources were developed and refined via two iterative cycles of session and resource piloting, feedback, reflection and refinement. Decision on delivery and content were made by stakeholders (young people, teachers, parents, paediatricians) and all sites. Materials were translated to local languages. Site-specific adaptations to the language, content and supporting resources were made., Results: The "PRE-STARt" programme is eight 90-min interactive sessions with supporting curriculum and resources. Iterative development work provided valuable feedback on programme content and delivery., Conclusion: Reporting on the intervention development process, which includes stakeholder input, could yield a flexible approach for use in this emerging 'at risk' groups and their families.
- Published
- 2021
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26. Metabolic Footprint, towards Understanding Type 2 Diabetes beyond Glycemia.
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Pina AF, Patarrão RS, Ribeiro RT, Penha-Gonçalves C, Raposo JF, Gardete-Correia L, Duarte R, M Boavida J, L Medina J, Henriques R, and Macedo MP
- Abstract
Type 2 diabetes (T2D) heterogeneity is a major determinant of complications risk and treatment response. Using cluster analysis, we aimed to stratify glycemia within metabolic multidimensionality and extract pathophysiological insights out of metabolic profiling. We performed a cluster analysis to stratify 974 subjects (PREVADIAB2 cohort) with normoglycemia, prediabetes, or non-treated diabetes. The algorithm was informed by age, anthropometry, and metabolic milieu (glucose, insulin, C-peptide, and free fatty acid (FFA) levels during the oral glucose tolerance test OGTT). For cluster profiling, we additionally used indexes of metabolism mechanisms (e.g., tissue-specific insulin resistance, insulin clearance, and insulin secretion), non-alcoholic fatty liver disease (NAFLD), and glomerular filtration rate (GFR). We found prominent heterogeneity within two optimal clusters, mainly representing normometabolism (Cluster-I) or insulin resistance and NAFLD (Cluster-II), at higher granularity. This was illustrated by sub-clusters showing similar NAFLD prevalence but differentiated by glycemia, FFA, and GFR (Cluster-II). Sub-clusters with similar glycemia and FFA showed dissimilar insulin clearance and secretion (Cluster-I). This work reveals that T2D heterogeneity can be captured by a thorough metabolic milieu and mechanisms profiling- metabolic footprint . It is expected that deeper phenotyping and increased pathophysiology knowledge will allow to identify subject's multidimensional profile, predict their progression, and treat them towards precision medicine.
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- 2020
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27. Insights from qualitative research on NAFLD awareness with a cohort of T2DM patients: time to go public with insulin resistance?
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Alemany-Pagès M, Moura-Ramos M, Araújo S, Macedo MP, Ribeiro RT, do Ó D, Ramalho-Santos J, and Azul AM
- Subjects
- Age Factors, Aged, Aged, 80 and over, Cohort Studies, Female, Humans, Male, Middle Aged, Non-alcoholic Fatty Liver Disease prevention & control, Portugal epidemiology, United States, United States Public Health Service, Diabetes Mellitus, Type 2, Insulin Resistance, Non-alcoholic Fatty Liver Disease epidemiology
- Abstract
Background: It is estimated that around 70% of Type 2 Diabetes Mellitus patients (T2DM) have Non-Alcoholic Fatty Liver Disease (NAFLD). Awareness and education are amongst the major shortcomings of the public health response to the increasing threat of NAFLD. Characterizing the specific NAFLD-related information needs of particular high-risk metabolic communities, for instance, T2DM patients, might aid in the development of evidence-based health promotion strategies, ultimately promoting NAFLD-awareness, treatment adherence and therapeutic success rates., Methods: Semi-structured interviews with T2DM patients were conducted to gain insight into their awareness of NAFLD, including its relationship with insulin resistance and T2DM., Results: Awareness of NAFLD as a disease entity, as well as its progression to end-stage liver disease or its relationship with other metabolic conditions, including insulin resistance and T2DM was low. Surveillance behaviours were also suboptimal and perceptions on the self-management knowledge and praxis regarding lifestyle intervention components of T2DM treatment seemed detached from those of NAFLD., Conclusions: Our findings could inform the integration of NAFLD-related content in T2DM health promotion strategies. Rising awareness on NAFLD progression and its relationship with T2DM using culturally and community-relevant constructs might facilitate the development of primary and secondary prevention programmes to promote the adherence to lifestyle interventions by influencing NAFLD threat perceptions.
- Published
- 2020
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28. Anandamide Is Related to Clinical and Cardiorespiratory Benefits of Aerobic Exercise Training in Migraine Patients: A Randomized Controlled Clinical Trial.
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Oliveira AB, Ribeiro RT, Mello MT, Tufik S, and Peres MFP
- Abstract
Introduction: Since endocannabinoids have been implicated in migraine pathophysiology, we conducted a randomized, controlled clinical trial to test the effects of a 12-week aerobic exercise intervention on plasma anandamide (AEA) and its relation with clinical, psychological, and cardiorespiratory outcomes. Materials and Methods: Episodic migraine patients taking no preventive drugs and nonheadache individuals were recruited from Hospital São Paulo and a tertiary headache clinic between March 2012 and March 2015. Participants were randomly assigned to receive aerobic exercise or enter the waitlist. Primary outcome was changes in plasma AEA; secondary outcome was number of days with migraine/month; and other clinical variables, mood scores, and cardiorespiratory fitness were chosen as tertiary outcomes. Measurements were taken on headache-free days. Data were analyzed by generalized linear models. Discussion: Fifty participants concluded the study (mean±SD age=36.2±10.9, and BMI=26.5±4.5). The plasma AEA reduced in migraine exercise ( p <0.05) and control exercise groups ( p <0.01). The number of days with migraine ( p <0.01), migraine attacks ( p <0.05), and abortive medication used ( p <0.05) reduced in the migraine exercise group, whereas cardiorespiratory fitness increased in migraine exercise and control exercise groups (both p <0.05). Anxiety, depression, anger, and fatigue scores improved in the migraine exercise group ( p <0.05 for all). Significant correlations between reduction in abortive medication used and cardiorespiratory fitness ( r =-0.81 p <0.001), and reduced AEA ( r =0.68 p <0.05) were found. Conclusions: This study suggests that peripheral AEA metabolism may be partly linked to the clinical and cardiorespiratory benefits of regular aerobic exercise in migraine patients. Trials registration: #NCT01972607., Competing Interests: No competing financial interests exist., (Copyright 2019, Mary Ann Liebert, Inc., publishers.)
- Published
- 2019
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29. Microbicidal Dispersions and Coatings from Hybrid Nanoparticles of Poly (Methyl Methacrylate), Poly (Diallyl Dimethyl Ammonium) Chloride, Lipids, and Surfactants.
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Ribeiro RT, Galvão CN, Betancourt YP, Mathiazzi BI, and Carmona-Ribeiro AM
- Subjects
- Anti-Bacterial Agents pharmacology, Microbial Sensitivity Tests, Staphylococcus aureus drug effects, Allyl Compounds chemistry, Anti-Bacterial Agents chemistry, Anti-Infective Agents chemistry, Lipids chemistry, Nanoparticles chemistry, Polymers chemistry, Polymethyl Methacrylate chemistry, Quaternary Ammonium Compounds chemistry, Surface-Active Agents chemistry
- Abstract
Hybrid and antimicrobial nanoparticles (NPs) of poly (methyl methacrylate) (PMMA) in the presence of poly (diallyl dimethyl ammonium) chloride (PDDA) were previously obtained by emulsion polymerization in absence of surfactant with low conversion. In the presence of amphiphiles such as cetyl trimethyl ammonium bromide (CTAB), dioctadecyl dimethyl ammonium bromide (DODAB) or soybean lecithin, we found that conversion increased substantially. In this work, the effect of the amphiphiles on the NPs core-shell structure and on the antimicrobial activity of the NPs was evaluated. NPs dispersions casted on silicon wafers, glass coverslips or polystyrene substrates were also used to obtain antimicrobial coatings. Methods for characterizing the dispersions and coatings were based on scanning electron microscopy, dynamic light scattering, determination of thickness, rugosity, and wettability for the coatings and determination of colony-forming unities (log CFU/mL) of microbia after 1 h interaction with the coatings or dispersions. The amphiphiles used during PMMA/PDDA/amphiphile NPs synthesis reduced the thickness of the NPs PDDA shell surrounding each particle. The antimicrobial activity of the dispersions and coatings were due to PDDA-the amphiphiles were either washed out by dialysis or remained in the PMMA polymeric core of the NPs. The most active NPs and coatings were those of PMMA/PDDA/CTAB-the corresponding coatings showed the highest rugosity and total surface area to interact with the microbes. The dispersions and coatings obtained by casting of the NPs dispersions onto silicon wafers were hydrophilic and exhibited microbicidal activity against Escherichia coli , Staphylococcus aureus , and Candida albicans . In addition, a major effect of reduction in particle size revealed the suitability of nanometric and cationic NPs (sizes below 100 nm) represented by PMMA/PDDA/CTAB NPs to yield maximal microbicidal activity from films and dispersions against all microbia tested. The reduction of cell viability by coatings and dispersions amounted to 6-8 logs from [PDDA] ≥ minimal microbicidal concentration.
- Published
- 2019
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30. l-Carnitine prevents oxidative stress in striatum of glutaryl-CoA dehydrogenase deficient mice submitted to lysine overload.
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Guerreiro G, Amaral AU, Ribeiro RT, Faverzani J, Groehs AC, Sitta A, Deon M, Wajner M, and Vargas CR
- Subjects
- Amino Acid Metabolism, Inborn Errors metabolism, Amino Acid Metabolism, Inborn Errors pathology, Amino Acid Metabolism, Inborn Errors veterinary, Animals, Brain Diseases, Metabolic metabolism, Brain Diseases, Metabolic pathology, Brain Diseases, Metabolic veterinary, Carnitine analogs & derivatives, Carnitine metabolism, Diet veterinary, Disease Models, Animal, Glutaryl-CoA Dehydrogenase deficiency, Glutaryl-CoA Dehydrogenase metabolism, Glutathione Peroxidase metabolism, Lysine blood, Mice, Mice, Knockout, Reactive Oxygen Species metabolism, Superoxide Dismutase metabolism, Carnitine pharmacology, Corpus Striatum metabolism, Glutaryl-CoA Dehydrogenase genetics, Lysine pharmacology, Oxidative Stress drug effects
- Abstract
The deficiency of the enzyme glutaryl-CoA dehydrogenase leads to predominant accumulation of glutaric acid (GA) in the organism and is known as glutaric acidemia type I (GA1). Despite the mechanisms of brain damage involved in GA1 are not fully understood, oxidative stress may be involved in this process. Treatment is based on protein/lysine (Lys) restriction and l-carnitine (L-car) supplementation. L-car was recently shown to have an important antioxidant role. A knockout mice model (Gcdh
-/- ) submitted to a dietary overload of Lys was developed to better understand the GA1 pathogenesis. In this study, we evaluated L-car and glutarylcarnitine levels, the lipid and protein damage, reactive oxygen species (ROS) production and antioxidant enzymes activities in striatum of Gcdh-/- and wild-type (WT) mice. We also determined the effect of the L-car treatment on these parameters. Thirty-day-old Gcdh-/- and WT mice were fed a normal chow (0.9% Lys) or submitted to a high Lys diet (4.7%) for 72 h. Additionally, these animals were administered with three intraperitoneal injections of saline or L-car in different times. Gcdh-/- mice were deficient in L-car and presented a higher glutarylcarnitine levels. They also presented lipid and protein damage, an increased ROS production and altered antioxidant enzymes compared to WT mice. Additionally, mice exposed to Lys overload presented higher alterations in these parameters than mice under normal diet, which were significantly decreased or normalized in those receiving L-car. Thus, we demonstrated a new beneficial effect of the L-car treatment attenuating or abolishing the oxidative stress process in Gcdh-/- mice., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2019
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31. Effects of combined training with different intensities on vascular health in patients with type 2 diabetes: a 1-year randomized controlled trial.
- Author
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Magalhães JP, Melo X, Correia IR, Ribeiro RT, Raposo J, Dores H, Bicho M, and Sardinha LB
- Subjects
- Antihypertensive Agents therapeutic use, Arterial Pressure, Brachial Artery diagnostic imaging, Carotid Arteries diagnostic imaging, Carotid Intima-Media Thickness, Diabetes Mellitus, Type 2 diagnosis, Diabetes Mellitus, Type 2 physiopathology, Diabetic Angiopathies diagnostic imaging, Diabetic Angiopathies physiopathology, Humans, Hypoglycemic Agents therapeutic use, Manometry, Portugal, Pulse Wave Analysis, Time Factors, Treatment Outcome, Vascular Stiffness, Brachial Artery physiopathology, Carotid Arteries physiopathology, Diabetes Mellitus, Type 2 therapy, Diabetic Angiopathies therapy, Hemodynamics, High-Intensity Interval Training, Resistance Training
- Abstract
Background: Exercise, when performed on a regular basis, is a well-accepted strategy to improve vascular function in patients with type 2 diabetes. However, the exercise intensity that yields maximal adaptations on structural and functional indices in patients with type 2 diabetes remains uncertain. Our objective was to analyze the impact of a 1-year randomized controlled trial of combined high-intensity interval training (HIIT) with resistance training (RT) vs. a combined moderate continuous training (MCT) with RT on structural and functional arterial indices in patients with type 2 diabetes., Methods: Patients with type 2 diabetes (n = 80) were randomized into an exercise intervention with three groups: control, combined HIIT with RT and combined MCT with RT. The 1-year intervention had 3 weekly exercise sessions. High-resolution ultrasonography of the common carotid artery and central and peripheral applanation tonometry were used to assess the changes in structural and functional arterial indices. Generalized estimating equations were used to model the corresponding outcomes., Results: After adjusting the models for sex, baseline moderate-to-vigorous physical activity, and mean arterial pressure changes, while using the intention-to-treat analysis, a significant interaction was observed on the carotid intima-media thickness (cIMT) for both the MCT (β = - 4.25, p < 0.01) and HIIT group (β = - 3.61, p < 0.01). However, only the HIIT observed favorable changes from baseline to 1-year on peripheral arterial stiffness indices such as carotid radial arterial pulse wave velocity (β = - 0.10, p = 0.044), carotid to distal posterior tibial artery pulse wave velocity (β = - 0.14, p < 0.01), and on the distensibility coefficient (β = - 0.00, p < 0.01). No effect was found for hemodynamic variables after the intervention., Conclusions: Following a 1-year intervention in patients with type 2 diabetes, both the MCT and HIIT group reduced their cIMT, whereas only the HIIT group improved their peripheral arterial stiffness indices and distensibility coefficient. Taken together, HIIT may be a meaningful tool to improve long-term vascular complications in type 2 diabetes. Trial registration clinicaltrials.gov ID: NCT03144505.
- Published
- 2019
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32. Treatment of Recurrent Painful Ophthalmoplegic Neuropathy: A Case Where Pregabalin Was Successfully Employed.
- Author
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Zamproni LN, Ribeiro RT, and Cardeal M
- Abstract
Recurrent painful ophthalmoplegic neuropathy, previously known as ophthalmoplegic migraine, is a rare condition that affects children and young adults. Its cause and classification are still controversial and, consequently, there are no published treatment guidelines or consensus. Glucocorticoids seem to be beneficial for some patients, but there is no established treatment when failure of this therapy occurs. The aim of this study was to report a case where pregabalin was successfully used after failure of glucocorticoid therapy in a patient with recurrent painful ophthalmoplegic neuropathy.
- Published
- 2019
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33. Antimicrobial Coatings from Hybrid Nanoparticles of Biocompatible and Antimicrobial Polymers.
- Author
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Galvão CN, Sanches LM, Mathiazzi BI, Ribeiro RT, Petri DFS, and Carmona-Ribeiro AM
- Subjects
- Colloids, Escherichia coli drug effects, Microbial Sensitivity Tests, Nanoparticles ultrastructure, Polyethylenes pharmacology, Polymethyl Methacrylate pharmacology, Quaternary Ammonium Compounds pharmacology, Solvents, Staphylococcus aureus drug effects, Surface-Active Agents pharmacology, Anti-Bacterial Agents pharmacology, Coated Materials, Biocompatible pharmacology, Nanoparticles chemistry, Polymers pharmacology
- Abstract
Hybrid nanoparticles of poly(methylmethacrylate) synthesized in the presence of poly (diallyldimethyl ammonium) chloride by emulsion polymerization exhibited good colloidal stability, physical properties, and antimicrobial activity but their synthesis yielded poor conversion. Here we create antimicrobial coatings from casting and drying of the nanoparticles dispersions onto model surfaces such as those of silicon wafers, glass coverslips, or polystyrene sheets and optimize conversion using additional stabilizers such as cetyltrimethyl ammonium bromide, dioctadecyldimethyl ammonium bromide, or soybean lecithin during nanoparticles synthesis. Methodology included dynamic light scattering, determination of wettability, ellipsometry of spin-coated films, scanning electron microscopy, and determination of colony forming unities (log CFU/mL) of bacteria after 1 h interaction with the coatings. The additional lipids and surfactants indeed improved nanoparticle synthesis, substantially increasing the conversion rates by stabilizing the monomer droplets in dispersion during the polymerization. The coatings obtained by spin-coating or casting of the nanoparticles dispersions onto silicon wafers were hydrophilic with contact angles increasing with the amount of the cationic polymer in the nanoparticles. Against Escherichia coli and Staphylococcus aureus , bacteria cell counts were reduced by approximately 7 logs upon interaction with the coatings, revealing their potential for several biotechnological and biomedical applications.
- Published
- 2018
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34. Screening diabetes mellitus 2 based on electronic health records using temporal features.
- Author
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Pimentel A, Carreiro AV, Ribeiro RT, and Gamboa H
- Subjects
- Area Under Curve, Blood Glucose analysis, Diabetes Mellitus, Type 2 classification, Female, Glycated Hemoglobin analysis, Humans, Male, Mass Screening standards, Middle Aged, Predictive Value of Tests, ROC Curve, Risk Factors, Diabetes Mellitus, Type 2 diagnosis, Electronic Health Records statistics & numerical data, Mass Screening methods
- Abstract
The prevalence of type 2 diabetes mellitus is increasing worldwide. Current methods of treating diabetes remain inadequate, and therefore, prevention with screening methods is the most appropriate process to reduce the burden of diabetes and its complications. We propose a new prognostic approach for type 2 diabetes mellitus based on electronic health records without using the current invasive techniques that are related to the disease (e.g. glucose level or glycated hemoglobin (HbA1c)). Our methodology is based on machine learning frameworks with data enrichment using temporal features. As as result our predictive model achieved an area under the receiver operating characteristics curve with a random forest classifier of 84.22 percent when including data information from 2009 to 2011 to predict diabetic patients in 2012, 83.19 percent when including temporal features, and 83.72 percent after applying temporal features and feature selection. We conclude that he pathology prediction is possible and efficient using the patient's progression information over the years and without using the invasive techniques that are currently used for type 2 diabetes mellitus classification.
- Published
- 2018
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35. Biomimetic Cationic Nanoparticles Based on Silica: Optimizing Bilayer Deposition from Lipid Films.
- Author
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Ribeiro RT, Braga VHA, and Carmona-Ribeiro AM
- Abstract
The optimization of bilayer coverage on particles is important for a variety of biomedical applications, such as drug, vaccine, and genetic material delivery. This work aims at optimizing the deposition of cationic bilayers on silica over a range of experimental conditions for the intervening medium and two different assemblies for the cationic lipid, namely, lipid films or pre-formed lipid bilayer fragments. The lipid adsorption on silica in situ over a range of added lipid concentrations was determined from elemental analysis of carbon, hydrogen, and nitrogen and related to the colloidal stability, sizing, zeta potential, and polydispersity of the silica/lipid nanoparticles. Superior bilayer deposition took place from lipid films, whereas adsorption from pre-formed bilayer fragments yielded limiting adsorption below the levels expected for bilayer adsorption., Competing Interests: The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.
- Published
- 2017
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36. Cationic Nanostructures against Foodborne Pathogens.
- Author
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Carrasco LD, Bertolucci R Jr, Ribeiro RT, Sampaio JL, and Carmona-Ribeiro AM
- Published
- 2016
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37. Syndrome of Hepatic Cirrhosis, Dystonia, Polycythemia, and Hypermanganesemia Caused by Mutations in SLC30A10, a Manganese Transporter in Man.
- Author
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Tuschl K, Clayton PT, Gospe SM Jr, Gulab S, Ibrahim S, Singhi P, Aulakh R, Ribeiro RT, Barsottini OG, Zaki MS, Del Rosario ML, Dyack S, Price V, Rideout A, Gordon K, Wevers RA, Chong WK, and Mills PB
- Published
- 2016
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38. In vitro antifungal activity of four chemotypes of Lippia alba (Verbenaceae) essential oils against Alternaria solani (Pleosporeaceae) isolates.
- Author
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Tomazoni EZ, Pansera MR, Pauletti GF, Moura S, Ribeiro RT, and Schwambach J
- Subjects
- Alternaria drug effects, Lippia chemistry, Oils, Volatile pharmacology, Plant Oils pharmacology
- Abstract
Several volatile natural compounds produced by plant secondary metabolism have been proven to present antimicrobial action, enabling their use in phytopathogen control. They also present low environmental impact when compared to conventional pesticides. Essential oils contain these compounds and can be found in several plant species, such as Lippia alba (Mill.) N.E. Brown (Verbenaceae). Essential oils of four chemotypes of L. alba, characterized by their major compounds, namely camphor, citral, linalool and camphor/1,8-cineole, were tested against the phytopathogen Alternaria solani Sorauer (Pleosporaceae), which causes early blight on tomatoes and is responsible for great economic losses regarding production. Essential oils antifungal action was tested in vitro using potato dextrose agar medium with essential oil concentrations at 0.1, 0.5, 1.0, 1.5 and 2.0 µL mL-1. The chemotype that had the best performance was citral, showing significant inhibition compared to the others, starting at the 0.5 µL mL-1 concentration. The essential oil belonging to the linalool chemotype was efficient starting at the 1.5 µL mL-1 concentration. Conversely, the camphor chemotype did not show any action against the phytopathogen. Moreover, the essential oils had no remarkable effect on tomato germination and growth. In conclusion, these essential oils presented fungicidal action against A. solani.
- Published
- 2016
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39. Diabetes hinders community-acquired pneumonia outcomes in hospitalized patients.
- Author
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Martins M, Boavida JM, Raposo JF, Froes F, Nunes B, Ribeiro RT, Macedo MP, and Penha-Gonçalves C
- Abstract
Objectives: This study aimed to estimate the prevalence of diabetes mellitus (DM) in hospitalized patients with community-acquired pneumonia (CAP) and its impact on hospital length of stay and in-hospital mortality., Research Design and Methods: We carried out a retrospective, nationwide register analysis of CAP in adult patients admitted to Portuguese hospitals between 2009 and 2012. Anonymous data from 157 291 adult patients with CAP were extracted from the National Hospital Discharge Database and we performed a DM-conditioned analysis stratified by age, sex and year of hospitalization., Results: The 74 175 CAP episodes that matched the inclusion criteria showed a high burden of DM that tended to increase over time, from 23.7% in 2009 to 28.1% in 2012. Interestingly, patients with CAP had high DM prevalence in the context of the national DM prevalence. Episodes of CAP in patients with DM had on average 0.8 days longer hospital stay as compared to patients without DM (p<0.0001), totaling a surplus of 15 370 days of stay attributable to DM in 19 212 admissions. In-hospital mortality was also significantly higher in patients with CAP who have DM (15.2%) versus those who have DM (13.5%) (p=0.002)., Conclusions: Our analysis revealed that DM prevalence was significantly increased within CAP hospital admissions, reinforcing other studies' findings that suggest that DM is a risk factor for CAP. Since patients with CAP who have DM have longer hospitalization time and higher mortality rates, these results hold informative value for patient guidance and healthcare strategies.
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- 2016
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40. Use of Electronic Health Records and Geographic Information Systems in Public Health Surveillance of Type 2 Diabetes: A Feasibility Study.
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Laranjo L, Rodrigues D, Pereira AM, Ribeiro RT, and Boavida JM
- Abstract
Background: Data routinely collected in electronic health records (EHRs) offer a unique opportunity to monitor chronic health conditions in real-time. Geographic information systems (GIS) may be an important complement in the analysis of those data., Objective: The aim of this study was to explore the feasibility of using primary care EHRs and GIS for population care management and public health surveillance of chronic conditions, in Portugal. Specifically, type 2 diabetes was chosen as a case study, and we aimed to map its prevalence and the presence of comorbidities, as well as to identify possible populations at risk for cardiovascular complications., Methods: Cross-sectional study using individual-level data from 514 primary care centers, collected from three different types of EHRs. Data were obtained on adult patients with type 2 diabetes (identified by the International Classification of Primary Care [ICPC-2] code, T90, in the problems list). GISs were used for mapping the prevalence of diabetes and comorbidities (hypertension, dyslipidemia, and obesity) by parish, in the region of Lisbon and Tagus Valley. Descriptive statistics and multivariate logistic regression were used for data analysis., Results: We identified 205,068 individuals with the diagnosis of type 2 diabetes, corresponding to a prevalence of 5.6% (205,068/3,659,868) in the study population. The mean age of these patients was 67.5 years, and hypertension was present in 71% (144,938/205,068) of all individuals. There was considerable variation in diagnosed comorbidities across parishes. Diabetes patients with concomitant hypertension or dyslipidemia showed higher odds of having been diagnosed with cardiovascular complications, when adjusting for age and gender (hypertension odds ratio [OR] 2.16, confidence interval [CI] 2.10-2.22; dyslipidemia OR 1.57, CI 1.54-1.60)., Conclusions: Individual-level data from EHRs may play an important role in chronic disease surveillance, namely through the use of GIS. Promoting the quality and comprehensiveness of data, namely through patient involvement in their medical records, is crucial to enhance the feasibility and usefulness of this approach.
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- 2016
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41. Karyotype of Philodryas nattereri and Philodryas olfersii with a comparative analysis of the Dipsadidae family.
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Nery MD, Alves MA, Aquino HD, Nery EA, Bezerra LB, Ribeiro RT, and Monteiro HS
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- Animals, Chromosome Aberrations, Diploidy, Karyotype, Karyotyping, Snakes genetics
- Abstract
Cytogenetic studies of Philodryas nattereri and Philodryas olfersii revealed a diploid chromosome number 2n = 36 for both species (3 metacentrics, 4 submetacentrics, and 10 acrocentrics, with a fundamental number of 51 and 52, respectively). The results obtained are novel and similar to those previously described for species belonging to the Dipsadidae family. The conventional karyotype is also novel and divergent from other species of the Dipsadidae family, where a higher proportion of macrochromosomes predominate, revealing two distinct groups in this family. The data are reported and discussed considering the cytotaxonomy of the family. These results strongly support the current view that chromosomal alterations, such as centric fusion and Robertsonian's translocations, seems to support the distinct importance of chromosomal rearrangements in speciation within this group.
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- 2015
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42. Classification and staging of chronic liver disease from multimodal data.
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Ribeiro RT, Marinho RT, and Sanches JM
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- Bayes Theorem, Case-Control Studies, Databases, Factual, Hepatitis, Chronic, Humans, Liver diagnostic imaging, Liver pathology, Liver Cirrhosis, Liver Diseases diagnostic imaging, Liver Diseases pathology, Support Vector Machine, Ultrasonography, Wavelet Analysis, Diagnosis, Computer-Assisted methods, Liver Diseases classification, Liver Diseases diagnosis
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Chronic liver disease (CLD) is most of the time an asymptomatic, progressive, and ultimately potentially fatal disease. In this study, an automatic hierarchical procedure to stage CLD using ultrasound images, laboratory tests, and clinical records are described. The first stage of the proposed method, called clinical based classifier (CBC), discriminates healthy from pathologic conditions. When nonhealthy conditions are detected, the method refines the results in three exclusive pathologies in a hierarchical basis: 1) chronic hepatitis; 2) compensated cirrhosis; and 3) decompensated cirrhosis. The features used as well as the classifiers (Bayes, Parzen, support vector machine, and k -nearest neighbor) are optimally selected for each stage. A large multimodal feature database was specifically built for this study containing 30 chronic hepatitis cases, 34 compensated cirrhosis cases, and 36 decompensated cirrhosis cases, all validated after histopathologic analysis by liver biopsy. The CBC classification scheme outperformed the nonhierachical one against all scheme, achieving an overall accuracy of 98.67% for the normal detector, 87.45% for the chronic hepatitis detector, and 95.71% for the cirrhosis detector.
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- 2013
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43. Factors associated with acute medication overuse in chronic migraine patients.
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Guendler VZ, Mercante JP, Ribeiro RT, Zukerman E, and Peres MF
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- Adolescent, Adult, Aged, Body Mass Index, Brazil, Chronic Disease, Educational Status, Female, Humans, Male, Middle Aged, Risk Factors, Surveys and Questionnaires, Young Adult, Analgesics adverse effects, Mental Disorders chemically induced, Migraine Disorders drug therapy
- Abstract
Objective: To evaluate the prevalence of psychiatric disorders in patients diagnosed with chronic migraine with and without acute medication overuse., Methods: Seventy-two volunteers were recruited from a Family Health Program of the Paraisópolis community in São Paulo (SP), Brazil. These patients were submitted to a detailed headache questionnaire. All participants were submitted to physical and neurological examinations. The following variables were analyzed: age, gender, education level, body mass index, type of overused medication, headache characteristics, and caffeine consumption, lifetime anxiety and mood disorders., Results: Out of 72 patients, 50 (69%) had chronic migraine with medication overuse, and 22 (31%) had chronic migraine without medication overuse. Factors such as age, gender, education level, body mass index, type of overused medication, headache characteristics, and caffeine consumption were not significantly different between the two studied groups. Lifetime anxiety and mood disorders were more common in patients with acute medication overuse (p=0.003 and p=0.045, respectively)., Conclusion: This study has shown a significant association among chronic migraine and medication overuse with lifetime mood and anxiety disorders in patients of the studied population. No association was found for other researched psychiatric disorders.
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- 2012
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44. Syndrome of hepatic cirrhosis, dystonia, polycythemia, and hypermanganesemia caused by mutations in SLC30A10, a manganese transporter in man.
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Tuschl K, Clayton PT, Gospe SM Jr, Gulab S, Ibrahim S, Singhi P, Aulakh R, Ribeiro RT, Barsottini OG, Zaki MS, Del Rosario ML, Dyack S, Price V, Rideout A, Gordon K, Wevers RA, Chong WK, and Mills PB
- Subjects
- Adolescent, Adult, Amino Acid Sequence, Brain metabolism, Cation Transport Proteins metabolism, Child, Child, Preschool, Chromosome Mapping methods, Female, Genetic Predisposition to Disease, Humans, Liver metabolism, Male, Manganese Poisoning metabolism, Molecular Sequence Data, Saccharomyces cerevisiae genetics, Sequence Alignment, Sequence Analysis, DNA, Young Adult, Zinc Transporter 8, Cation Transport Proteins genetics, Codon, Nonsense, Manganese metabolism, Manganese Poisoning genetics, Metabolic Diseases genetics, Metabolic Diseases metabolism, Mutation, Missense
- Abstract
Environmental manganese (Mn) toxicity causes an extrapyramidal, parkinsonian-type movement disorder with characteristic magnetic resonance images of Mn accumulation in the basal ganglia. We have recently reported a suspected autosomal recessively inherited syndrome of hepatic cirrhosis, dystonia, polycythemia, and hypermanganesemia in cases without environmental Mn exposure. Whole-genome mapping of two consanguineous families identified SLC30A10 as the affected gene in this inherited type of hypermanganesemia. This gene was subsequently sequenced in eight families, and homozygous sequence changes were identified in all affected individuals. The function of the wild-type protein and the effect of sequence changes were studied in the manganese-sensitive yeast strain Δpmr1. Expressing human wild-type SLC30A10 in the Δpmr1 yeast strain rescued growth in high Mn conditions, confirming its role in Mn transport. The presence of missense (c.266T>C [p.Leu89Pro]) and nonsense (c.585del [p.Thr196Profs(∗)17]) mutations in SLC30A10 failed to restore Mn resistance. Previously, SLC30A10 had been presumed to be a zinc transporter. However, this work has confirmed that SLC30A10 functions as a Mn transporter in humans that, when defective, causes Mn accumulation in liver and brain. This is an important step toward understanding Mn transport and its role in neurodegenerative processes., (Copyright © 2012 The American Society of Human Genetics. Published by Elsevier Inc. All rights reserved.)
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- 2012
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45. Migrainous infarction as a complication of sporadic hemiplegic migraine in childhood.
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Ribeiro RT, Pinto MM, Villa TR, Gamba LT, Tengan CH, and de Souza-Carvalho D
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- Cerebral Infarction diagnosis, Cerebral Infarction genetics, Child, Hemiplegia diagnosis, Hemiplegia genetics, Humans, Magnetic Resonance Imaging, Male, Migraine Disorders diagnosis, Migraine Disorders genetics, Cerebral Infarction etiology, Hemiplegia etiology, Migraine Disorders complications
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- 2009
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46. Trigeminal sensory neuropathy associated with systemic sclerosis: report of three Brazilian cases.
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Ribeiro RT, Fialho LM, de Souza LT, and Barsottini OG
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- Adult, Female, Humans, Male, Middle Aged, Multiple Sclerosis complications, Trigeminal Neuralgia etiology
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- 2009
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47. [Evolution of the prevalence of intestinal parasitosis among schoolchildren in Caxias do Sul, RS].
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Basso RM, Silva-Ribeiro RT, Soligo DS, Ribacki SI, Callegari-Jacques SM, and Zoppas BC
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- Adolescent, Animals, Brazil epidemiology, Child, Female, Helminthiasis parasitology, Humans, Intestinal Diseases, Parasitic parasitology, Prevalence, Protozoan Infections parasitology, Feces parasitology, Helminthiasis epidemiology, Intestinal Diseases, Parasitic epidemiology, Protozoan Infections epidemiology
- Abstract
Reports on the prevalence of intestinal parasitosis in Brazil have been local in nature, with descriptions of different populations, which makes comprehensive diagnosis difficult. With the aim of studying the variation in the prevalence of intestinal parasitosis among schoolchildren in Caxias do Sul, Rio Grande do Sul, over a 35-year period, 9,787 parasitological stool tests that had been performed using centrifugal sedimentation were evaluated. There were positive results from 5,655 samples (58%), and the most prevalent infestations were of Ascaris lumbricoides (47%), Trichuris trichiura (36%), Enterobius vermicularis (8%) and the protozoa Giardia lamblia (24%) and Entamoeba coli (20%). The overall prevalence diminished from 89% to 37%, indicating an average decrease of 1.4% per year. Reductions in prevalence were observed for Ascaris lumbricoides (61 to 26%) and Trichuris trichiura (38 to 18%). No significant change was observed for Giardia lamblia. The prevalence of Entamoeba coli increased from 29 to 46%. The decreases in helminth prevalence were probably due to infrastructure improvements and educational actions undertaken in schools.
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- 2008
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48. Glossopharyngeal neuralgia with syncope as a sign of neck cancer recurrence.
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Ribeiro RT, Souza NA, and Carvalho Dde S
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- Aged, Anticonvulsants therapeutic use, Carbamazepine therapeutic use, Glossopharyngeal Nerve Diseases diagnosis, Glossopharyngeal Nerve Diseases drug therapy, Humans, Male, Neoplasm Recurrence, Local diagnosis, Palliative Care, Pharyngeal Neoplasms diagnosis, Pharyngeal Neoplasms radiotherapy, Syncope diagnosis, Syncope drug therapy, Tomography, X-Ray Computed, Glossopharyngeal Nerve Diseases etiology, Neoplasm Recurrence, Local complications, Pharyngeal Neoplasms complications, Syncope etiology
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Glossopharyngeal neuralgia with syncope as a sign of neck cancer is a very rare condition. A review of the literature revealed only 29 cases formerly reported. We present the first Brazilian case of such association. A 68-year-old man presented with paroxysmal excruciating pain over the right side of the neck, sometimes followed by syncope. Given the suspicion of recurrent tumor from a previously treated neck malignancy, a computed tomography scan was performed and a right parapharyngeal tumor was shown. Pain and syncope were successfully controlled with carbamazepine and the patient underwent palliative radiotherapy.
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- 2007
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49. Hepatic-dependent and -independent insulin actions are impaired in the obese Zucker rat model.
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Afonso RA, Ribeiro RT, Fernandes AB, Patarrão RS, and Macedo MP
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- Animals, Atropine pharmacology, Insulin metabolism, Insulin Resistance, Liver drug effects, Liver innervation, Liver metabolism, Male, Nitric Oxide Synthase antagonists & inhibitors, Parasympathetic Nervous System drug effects, Parasympatholytics pharmacology, Rats, Rats, Zucker, Signal Transduction, Thinness, omega-N-Methylarginine pharmacology, Insulin physiology, Liver physiology, Obesity physiopathology
- Abstract
Objective: Whole-body insulin sensitivity (IS) depends on a hepatic pathway, involving parasympathetic activation and hepatic nitric oxide (NO) production. Both atropine and N-monomethyl-L-arginine (L-NMMA, NO synthase inhibitor) induce insulin resistance (IR). IR is associated with obesity. Because NO action was shown to be impaired in animal models of obesity, such as the obese Zucker rat (OZR), we tested the hypothesis that the hepatic-dependent pathway is diminished in OZR, resulting in IR., Research Methods and Procedures: Lean Zucker rats (LZRs) were used as OZR controls. IS was evaluated in terms of glucose disposal [milligrams of glucose per kilogram of body weight (bw)]. Two groups were submitted to two protocols. First, a control clamp was followed by a post-atropine (3 mg/kg intravenously) clamp. Second, after the control clamp, L-NMMA (0.73 mg/kg intraportally) was given, and a second clamp was performed. Hepatic-dependent IS was assessed by subtracting the response after atropine or L-NMMA from the basal response., Results: In the first protocol, basal IS was lower in OZR than in LZR (OZR, 73.7 +/- 14.2; LZR, 289.2 +/- 24.7 mg glucose/kg bw; p < 0.001), and atropine decreased IS in the same proportion for both groups (OZR, 41.3 +/- 8.0%; LZR, 40.1 +/- 6.5%). Equally, in the second protocol, OZR presented lower IS (OZR, 79.3 +/- 1.6; LZR, 287.4 +/- 22.7 mg glucose/kg bw; p < 0.001). L-NMMA induced IS inhibition in both groups (OZR, 48.3 +/- 6.6%; LZR, 46.4 +/- 4.1%), similar to that after atropine., Discussion: We show that the IR in OZR is due to similar impairment of both hepatic-dependent and -independent components of insulin action, suggesting the existence of a defect common to both pathways.
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- 2007
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50. Insulin resistance induced by sucrose feeding in rats is due to an impairment of the hepatic parasympathetic nerves.
- Author
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Ribeiro RT, Lautt WW, Legare DJ, and Macedo MP
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- Animals, Atropine pharmacology, Blood Glucose drug effects, Blood Glucose metabolism, Dietary Supplements, Injections, Intravenous, Insulin administration & dosage, Insulin pharmacology, Kinetics, Male, Mice, Models, Animal, Rats, Rats, Sprague-Dawley, Rats, Wistar, Dietary Sucrose pharmacology, Insulin Resistance physiology, Liver innervation, Parasympathetic Nervous System physiopathology
- Abstract
Aims/hypothesis: A considerable proportion of whole-body insulin-stimulated glucose uptake is dependent upon the hepatic insulin-sensitising substance (HISS) in a pathway mediated by the hepatic parasympathetic nerves (HPNs). We tested the hypothesis that a high-sucrose diet leads to the impairment of the HPN-dependent component of insulin action., Methods: We quantified insulin sensitivity using the rapid insulin sensitivity test, a modified euglycaemic clamp. Quantification of the HPN-dependent component was achieved by administration of a muscarinic receptor antagonist (atropine, 3 mg/kg)., Results: Insulin sensitivity was higher in standard-fed than in sucrose-fed Wistar rats (305.6+/-34.1 vs 193.9+/-13.7 mg glucose/kg body weight; p<0.005) and Sprague-Dawley rats (196.4+/-5.9 vs 95.5+/-16.3 mg glucose/kg body weight; p<0.01). The HPN-independent component was similar in the two diet groups. Insulin resistance was entirely due to an impairment of the HPN-dependent component in both Wistar rats (164.3+/-28.1 [standard-fed] vs 26.5+/-7.5 [sucrose-fed] mg glucose/kg body weight; p<0.0001) and Sprague-Dawley rats (111.7+/-9.5 vs 35.3+/-21.4 mg glucose/kg body weight; p<0.01). Furthermore, HPN-dependent insulin resistance in Sprague-Dawley rats was already evident after 2 weeks of a high-sucrose diet (28.5+/-7.6 [2 weeks], 35.3+/-21.4 [6 weeks], 17.9+/-5.4 [9 weeks] mg glucose/kg body weight) and was independent of the nature of sucrose supplementation (12.3+/-4.7 [solid] and 17.9+/-5.4 [liquid] mg glucose/kg body weight)., Conclusions/interpretation: Our results support the hypothesis that insulin resistance caused by sucrose feeding is due to an impairment of the HPN-dependent component of insulin action, leading to a dysfunction of the HISS pathway.
- Published
- 2005
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