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29 results on '"Ricciutelli, Massimo"'

1. Assessment and Comparison of Phytochemical Constituents and Biological Activities between Full Flowering and Late Flowering of Hypericum perforatum L.

Author

Piatti, Diletta, Marconi, Riccardo, Caprioli, Giovanni, Angeloni, Simone, Ricciutelli, Massimo, Zengin, Gokhan, Maggi, Filippo, Pagni, Luca, and Sagratini, Gianni

Subjects
HYPERICUM perforatum, SCIENTIFIC knowledge, PHENOLS, ANTIOXIDANT testing, NEURODEGENERATION, PHENOL oxidase, FLOWERING time
Abstract

This study assessed the impact of full and late flowering stages on the polyphenols and enzyme inhibitory properties of Hypericum perforatum from Poland. Recognizing the significance of phenolic compounds in disease prevention and melatonin's emerging protective role, we employed an UHPLC-MS/MS system to quantify 38 phenolic compounds, not typical of St. John's wort, and to develop a new method for melatonin quantification. Afterward, the extracts were tested for their antioxidant capabilities (using phosphomolybdenum, DPPH, ABTS, FRAP, CUPRAC and ferrous chelating assays). Moreover, we investigated enzymes (acetylcholinesterase, butyrylcholinesterase and tyrosinase) involved in neurodegenerative disorders and (α-amylase and α-glucosidase) in diabetes. This study recognized the importance of phenolic compounds in disease prevention and explored the emerging protective role of melatonin, taking into account the floral ontogeny of the plant. Indeed, the full-flowering plant contained the greatest concentration of phenolic compounds (a total of 65,276.5 µg/g): hyperoside (18,726.59 µg/g), isoquercitrin (11,895.02 µg/g) and delphindin-3.5-diglucoside (10,619.51 µg/g), and showed the highest inhibitory enzyme activity. Moreover, only full-flowering St. John's wort contained melatonin (40 ng/g). Our results offer additional perspectives on the chemical-biological characteristics of H. perforatum and scientific knowledge that testifies to the importance of considering plant growth conditions for the development of nutraceuticals. [ABSTRACT FROM AUTHOR]

Published
2023
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2. Fecal Protein Profile in Eight Dogs Suffering from Acute Uncomplicated Diarrhea before and after Treatment

Author

Cerquetella, Matteo, primary, Mangiaterra, Sara, additional, Rossi, Giacomo, additional, Gavazza, Alessandra, additional, Marchegiani, Andrea, additional, Sagratini, Gianni, additional, Ricciutelli, Massimo, additional, Angeloni, Simone, additional, Fioretti, Licia, additional, Marini, Carlotta, additional, Pucciarelli, Stefania, additional, and Vincenzetti, Silvia, additional

Published
2023
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6. Dual Anta-Inhibitors Targeting Protein Kinase CK1δ and A 2A Adenosine Receptor Useful in Neurodegenerative Disorders.

Author

Francucci, Beatrice, Angeloni, Simone, Dal Ben, Diego, Lambertucci, Catia, Ricciutelli, Massimo, Spinaci, Andrea, Smirnov, Aleksei, Volpini, Rosaria, Buccioni, Michela, and Marucci, Gabriella

Subjects
PROTEIN kinases, BILAYER lipid membranes, NEURODEGENERATION, INFLAMMATION, INTESTINAL absorption, ADENOSINES
Abstract

Currently, the number of patients with neurodegenerative pathologies is estimated at over one million, with consequences also on the economic level. Several factors contribute to their development, including overexpression of A2A adenosine receptors (A2AAR) in microglial cells and up-regulation and post-translational alterations of some casein kinases (CK), among them, CK-1δ. The aim of the work was to study the activity of A2AAR and CK1δ in neurodegeneration using in-house synthesized A2A/CK1δ dual anta-inhibitors and to evaluate their intestinal absorption. Experiments were performed on N13 microglial cells, which were treated with a proinflammatory CK cocktail to simulate an inflammatory state typical of neurodegenerative diseases. Results showed that the dual anta-inhibitors have the ability to counteract the inflammatory state, even if compound 2 is more active than compound 1. In addition, compound 2 displayed an important antioxidant effect similar to the reference compound ZM241385. Since many known kinase inhibitors are very often unable to cross lipid bilayer membranes, the ability of A2A/CK1δ double anta-inhibitors to cross the intestinal barrier was investigated by an everted gut sac assay. HPLC analysis revealed that both compounds are able to cross the intestinal barrier, making them promising candidates for oral therapy. [ABSTRACT FROM AUTHOR]

Published
2023
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11. Isofuranodiene, a Natural Sesquiterpene Isolated from Wild Celery (Smyrnium olusatrum L.), Protects Rats against Acute Ischemic Stroke

Author

Yousefi-Manesh, Hasan, primary, Dehpour, Ahmad Reza, additional, Shirooie, Samira, additional, Bagheri, Fariba, additional, Farrokhi, Vida, additional, Mousavi, Seyyedeh Elaheh, additional, Ricciutelli, Massimo, additional, Cappellacci, Loredana, additional, López, Víctor, additional, Maggi, Filippo, additional, and Petrelli, Riccardo, additional

Published
2021
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12. Faecal proteome in clinically healthy dogs and cats: Findings in pooled faeces from 10 cats and 10 dogs

Author

Cerquetella, Matteo, primary, Marchegiani, Andrea, additional, Mangiaterra, Sara, additional, Rossi, Giacomo, additional, Gavazza, Alessandra, additional, Tesei, Beniamino, additional, Spaterna, Andrea, additional, Sagratini, Gianni, additional, Ricciutelli, Massimo, additional, Polzonetti, Valeria, additional, Pucciarelli, Stefania, additional, and Vincenzetti, Silvia, additional

Published
2021
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13. Phytochemical Profile and Biological Activities of Crude and Purified Leonurus cardiaca Extracts

Author

Angeloni, Simone, primary, Spinozzi, Eleonora, additional, Maggi, Filippo, additional, Sagratini, Gianni, additional, Caprioli, Giovanni, additional, Borsetta, Germana, additional, Ak, Gunes, additional, Sinan, Kouadio Ibrahime, additional, Zengin, Gokhan, additional, Arpini, Sabrina, additional, Mombelli, Giacomo, additional, and Ricciutelli, Massimo, additional

Published
2021
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14. Studying GGDEF Domain in the Act: Minimize Conformational Frustration to Prevent Artefacts

Author

Mantoni, Federico, primary, Scribani Rossi, Chiara, additional, Paiardini, Alessandro, additional, Di Matteo, Adele, additional, Cappellacci, Loredana, additional, Petrelli, Riccardo, additional, Ricciutelli, Massimo, additional, Paone, Alessio, additional, Cutruzzolà, Francesca, additional, Giardina, Giorgio, additional, and Rinaldo, Serena, additional

Published
2021
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15. Chemical and Sensory Profiling of Monovarietal Extra Virgin Olive Oils from the Italian Marche Region

Author

Pacetti, Deborah, primary, Boarelli, Maria Chiara, additional, Giovannetti, Rita, additional, Ferraro, Stefano, additional, Conti, Paolo, additional, Alfei, Barbara, additional, Caprioli, Giovanni, additional, Ricciutelli, Massimo, additional, Sagratini, Gianni, additional, Fedeli, Donatella, additional, Gabbianelli, Rosita, additional, and Fiorini, Dennis, additional

Published
2020
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16. Proteomics of canine feces from healthy Boxer dogs: a pilot study

Author

Cerquetella, Matteo, Rossi, Giacomo, Spaterna, Andrea, Tesei, Beniamino, Gavazza, Alessandra, Marchegiani, Andrea, Pengo, Graziano, Scortichini, Luca, Felicioli, Antonio, Sagratini, Gianni, Ricciutelli, Massimo, and Vincenzetti, Silvia

Published
2019

17. Article Clinicopathological and Fecal Proteome Evaluations in 16 Dogs Presenting Chronic Diarrhea Associated with Lymphangiectasia.

Author

Rossi, Giacomo, Gavazza, Alessandra, Vincenzetti, Silvia, Mangiaterra, Sara, Galosi, Livio, Marchegiani, Andrea, Pengo, Graziano, Sagratini, Gianni, Ricciutelli, Massimo, and Cerquetella, Matteo

Subjects
DIARRHEA, C-reactive protein, TRANSTHYRETIN, CITRULLINE in the body, DOG diseases
Abstract

Canine intestinal lymphangiectasia (IL) is a condition characterized by variably severe gastrointestinal signs, frequently associated with laboratory abnormalities; the research for markers allowing a better understanding of the severity degree and/or obtaining an early diagnosis and/or monitoring is continuously progressing. In the present study, we investigated possible new diagnostic/follow-up markers in IL dogs, namely, serum C-reactive protein, serum bacterial lipopolysaccharide, serum cleaved cytokeratin 18, serum citrulline, and zonulin (in both serum and feces). A fecal proteomic study looking for possible confirmation and/or new marker candidates was also performed. All markers in both substrates, with the exception of serum citrulline, significantly differed between diseased and control dogs. Fecal proteomics allowed the retrieval of three proteins in IL dogs (Fc fragment of IgG-binding protein; transthyretin; proproteinase E) that were not previously found in clinically healthy subjects. Although further studies are needed, C-reactive protein, bacterial lipopolysaccharide, cleaved cytokeratin 18, and zonulin (in both serum and feces) resulted as promising markers for canine IL; similarly, fecal proteomics represents a road worthy of being pursued in the search for candidate biomarkers. [ABSTRACT FROM AUTHOR]

Published
2021
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21. Fecal Proteomic Analysis in Healthy Dogs and in Dogs Suffering from Food Responsive Diarrhea

Author

Cerquetella, Matteo, primary, Rossi, Giacomo, additional, Spaterna, Andrea, additional, Tesei, Beniamino, additional, Gavazza, Alessandra, additional, Pengo, Graziano, additional, Pucciarelli, Stefania, additional, Scortichini, Luca, additional, Sagratini, Gianni, additional, Ricciutelli, Massimo, additional, Marchegiani, Andrea, additional, and Vincenzetti, Silvia, additional

Published
2019
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22. Insights into the GTP-dependent allosteric control of c-di-GMP hydrolysis from the crystal structure of PA0575 protein from Pseudomonas aeruginosa

Author

Mantoni, Federico, Paiardini, Alessandro, Brunotti, Paolo, D'Angelo, Cecilia, Cervoni, Laura, Paone, Alessio, Cappellacci, Loredana, Petrelli, Riccardo, Ricciutelli, Massimo, Leoni, Livia, Rampioni, Giordano, Arcovito, Alessandro, Rinaldo, Serena, Cutruzzolà, Francesca, Giardina, Giorgio, Arcovito, Alessandro (ORCID:0000-0002-8384-4844), Mantoni, Federico, Paiardini, Alessandro, Brunotti, Paolo, D'Angelo, Cecilia, Cervoni, Laura, Paone, Alessio, Cappellacci, Loredana, Petrelli, Riccardo, Ricciutelli, Massimo, Leoni, Livia, Rampioni, Giordano, Arcovito, Alessandro, Rinaldo, Serena, Cutruzzolà, Francesca, Giardina, Giorgio, and Arcovito, Alessandro (ORCID:0000-0002-8384-4844)

Abstract

Bis-(3'-5')-cyclic diguanylic acid (c-di-GMP) belongs to the class of cyclic dinucleotides, key carriers of cellular information in prokaryotic and eukaryotic signal transduction pathways. In bacteria, the intracellular levels of c-di-GMP and their complex physiological outputs are dynamically regulated by environmental and internal stimuli, which control the antagonistic activities of diguanylate cyclases (DGCs) and c-di-GMP specific phosphodiesterases (PDEs). Allostery is one of the major modulators of the c-di-GMP-dependent response. Both the c-di-GMP molecule and the proteins interacting with this second messenger are characterized by an extraordinary structural plasticity, which has to be taken into account when defining and possibly predicting c-di-GMP-related processes. Here, we report a structure-function relationship study on the catalytic portion of the PA0575 protein from Pseudomonas aeruginosa, bearing both putative DGC and PDE domains. The kinetic and structural studies indicate that the GGDEF-EAL portion is a GTP-dependent PDE. Moreover, the crystal structure confirms the high degree of conformational flexibility of this module. We combined structural analysis and protein engineering studies to propose the possible molecular mechanism guiding the nucleotide-dependent allosteric control of catalysis; we propose that the role exerted by GTP via the GGDEF domain is to allow the two EAL domains to form a dimer, the species competent to enter PDE catalysis. This article is protected by copyright. All rights reserved.

Published
2018

25. Insights into the GTP‐dependent allosteric control of c‐di‐GMP hydrolysis from the crystal structure of PA0575 protein from Pseudomonas aeruginosa

Author

Mantoni, Federico, primary, Paiardini, Alessandro, additional, Brunotti, Paolo, additional, D'Angelo, Cecilia, additional, Cervoni, Laura, additional, Paone, Alessio, additional, Cappellacci, Loredana, additional, Petrelli, Riccardo, additional, Ricciutelli, Massimo, additional, Leoni, Livia, additional, Rampioni, Giordano, additional, Arcovito, Alessandro, additional, Rinaldo, Serena, additional, Cutruzzolà, Francesca, additional, and Giardina, Giorgio, additional

Published
2018
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26. SPME-GC-MS analysis of commercial henna samples (Lawsonia inermis L.)

Author

Ricciutelli, Massimo, Maggi, Filippo, Mengoni, Tamara, Peregrina, Dolores Vargas, Censi, Roberta, Cortese, Manuela, and Martino, Piera Di

Abstract

The aim of this work was to provide a characterisation of volatile constituents from different commercial batches of henna (Lawsonia inermis) leaves of different geographic origin. Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography–mass spectrometry (GC–MS) was used for the purpose. A total of 72 components were identified by GC–MS in the headspace of different henna samples which proved to differ considerably from each other, because they were characterised by different classes of components, mainly aliphatic compounds (9.0–64.7%), terpenoids (5.8–45.5%) and aromatics (7.9–45.2%), with alkanes (0.9–18.5%), aldehydes (2.1–18.8%) and carboxylic acids (3.1–29.3%), monoterpenes (3.4–30.0%) and sesquiterpenes (0.8–23.7%) and phenyl propanoids (0.6–43.1%), being the most abundant, respectively. Major representatives of these groups were n-hexadecane (0.5–4.7%), (2E)-hexenal (0.5–11.7%) and acetic acid (2.8–24.5%), limonene (0.8–14.7%), carvol (3.8–7.1%), geranyl acetone (1.4–7.9%) and (E)-caryophyllene (3.3–8.4%), and (E)-anethole (0.6–35.0%), respectively. We assume that factors such as the manufacturing process, the storage conditions and the different geographic origin of the samples may contribute to such variability.

Published
2015
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27. Chemical and microbiological stability studies of an aqueous solution of pravastatin sodium salt for drug therapy of the dysphagic patients

Author

Bonacucina, Giulia, primary, Logrippo, Serena, additional, Cespi, Marco, additional, Ganzetti, Roberta, additional, Casettari, Luca, additional, Sestili, Matteo, additional, Perinelli, Diego Romano, additional, Ricciutelli, Massimo, additional, Marziali, Antonella, additional, Polidori, Carlo, additional, and Palmieri, Giovanni Filippo, additional

Published
2016
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28. Insights into the GTP-dependent allosteric control of c-di-GMP hydrolysis from the crystal structure of PA0575 protein from Pseudomonas aeruginosa

Author

Serena Rinaldo, Federico Mantoni, Alessio Paone, Alessandro Paiardini, Laura Cervoni, Riccardo Petrelli, Giordano Rampioni, Loredana Cappellacci, Alessandro Arcovito, Paolo Brunotti, Massimo Ricciutelli, Francesca Cutruzzolà, Giorgio Giardina, Livia Leoni, Cecilia D'Angelo, Mantoni, Federico, Paiardini, Alessandro, Brunotti, Paolo, D'Angelo, Cecilia, Cervoni, Laura, Paone, Alessio, Cappellacci, Loredana, Petrelli, Riccardo, Ricciutelli, Massimo, Leoni, Livia, Rampioni, Giordano, Arcovito, Alessandro, Rinaldo, Serena, Cutruzzolà, Francesca, and Giardina, Giorgio

Subjects
0301 basic medicine, GTP', Protein Conformation, Two-hybrid screening, 030106 microbiology, Allosteric regulation, Crystallography, X-Ray, Biochemistry, 03 medical and health sciences, Allosteric Regulation, Bacterial Proteins, Hydrolase, GTP, RmcA, c-di-GMP, hybrid protein, phosphodiesterase, Settore BIO/10 - BIOCHIMICA, Molecular Biology, Cyclic GMP, Chemistry, Phosphoric Diester Hydrolases, Hydrolysis, Cell Biology, Protein engineering, GTP, RmcA, c-di-GMP, hybrid protein, phosphodiesterase, Pseudomonas, GGDEF domain, 030104 developmental biology, Second messenger system, Pseudomonas aeruginosa, Biophysics, Guanosine Triphosphate, Signal transduction, Protein Multimerization
Abstract

Bis-(3'-5')-cyclic diguanylic acid (c-di-GMP) belongs to the class of cyclic dinucleotides, key carriers of cellular information in prokaryotic and eukaryotic signal transduction pathways. In bacteria, the intracellular levels of c-di-GMP and their complex physiological outputs are dynamically regulated by environmental and internal stimuli, which control the antagonistic activities of diguanylate cyclases (DGCs) and c-di-GMP specific phosphodiesterases (PDEs). Allostery is one of the major modulators of the c-di-GMP-dependent response. Both the c-di-GMP molecule and the proteins interacting with this second messenger are characterized by an extraordinary structural plasticity, which has to be taken into account when defining and possibly predicting c-di-GMP-related processes. Here, we report a structure-function relationship study on the catalytic portion of the PA0575 protein from Pseudomonas aeruginosa, bearing both putative DGC and PDE domains. The kinetic and structural studies indicate that the GGDEF-EAL portion is a GTP-dependent PDE. Moreover, the crystal structure confirms the high degree of conformational flexibility of this module. We combined structural analysis and protein engineering studies to propose the possible molecular mechanism guiding the nucleotide-dependent allosteric control of catalysis; we propose that the role exerted by GTP via the GGDEF domain is to allow the two EAL domains to form a dimer, the species competent to enter PDE catalysis.

Published
2018

29. Fecal Proteomic Analysis in Healthy Dogs and in Dogs Suffering from Food Responsive Diarrhea.

Author

Cerquetella M, Rossi G, Spaterna A, Tesei B, Gavazza A, Pengo G, Pucciarelli S, Scortichini L, Sagratini G, Ricciutelli M, Marchegiani A, and Vincenzetti S

Subjects
Animals, Chromatography, Liquid, Dog Diseases epidemiology, Dogs, Proteomics methods, Tandem Mass Spectrometry, Animal Feed microbiology, Diarrhea veterinary, Dog Diseases metabolism, Dog Diseases microbiology, Feces chemistry, Foodborne Diseases veterinary, Proteome
Abstract

Different laboratory markers are routinely used in the diagnosis and management of gastrointestinal (GI) disease in dogs. In the present study, starting from feces from both healthy dogs and dogs suffering from food responsive diarrhea (FRD), we tried to find proteins differently expressed in the two groups of dogs, by using a proteomic approach. Interestingly, we found that the immunoglobulin J-chain isoform 1 (species: Canis lupus familiaris ) was identified only in diseased dogs (not in healthy). J-chain combines especially IgA monomers to IgA dimers and plays a crucial role for their secretions into mucosal interface. Being the first study of that kind in the dog, it is only possible to hypothesize that their presence could be likely due to an increased activation of the immune system or to a mucosal damage or both in FRD patients. Similarly, it is still impossible to assess whether this protein could be used as diagnostic/prognostic marker of GI disease; however, this study represents a promising first step toward fecal proteomics in canine GI disorders.

Published
2019
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