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1. Contrast efficacy of novel phase convertible nanodroplets for safe CEUS imaging

Author

R. Riaz, S. Shafiq, M. Fatima, M. A. Siddique, S. Shah, and S. R. Abbas

Subjects
Nanodroplets, Echocardiography, Ultrasound, Sonovue, Poly glycerol sebacate, Contrast agent, Medicine, Science
Abstract

Abstract Microbubble contrast agents in ultrasound/echocardiography are used to increase the echogenicity of the target tissues, thereby raising the contrast resolution of the resultant image. Recently, the trend has shifted toward the development of phase-convertible nanodroplets as ultrasound contrast agents due to their promising theragnostic potential by switching capability at the active site. Herein, we fabricated pre-PGS- perfluoropentane phase convertible nanodroplets and checked their in vitro and in vivo enhancement and safety profile. For this, we performed experiments on 20 male Wistar rats and 2 dogs. Biochemical assays of both rats and dogs included complete blood profiles, liver function tests, and renal function tests. For rat vitals, monitoring and histopathological analysis were also performed. Converted nanodroplets showed excellent contrast enhancement, better than Sonovue upon in vitro testing, with an enhancement time of up to 14 min. In vivo, experiments showed comparable opacification of the ventricles of both rats and dogs. All biochemical assays remained within the normal range during the study period. The histopathological analysis did not show any signs of drug-induced toxicity, showing the safety of these nanodroplets. Pre-PGS-PFP nanodroplets hold great potential for use in echocardiography and abdominal imaging in both human and veterinary applications after clinical trials.

Published
2024
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2. Tuberculosis detection from raw sputum samples using Au-electroplated screen-printed electrodes as E-DNA sensor

Author

M. N. Sharif, S. Taufiq, M. Sohail, and S. R. Abbas

Subjects
IS-6110, mtb detection, tuberculosis, screen printed electrode (SPE), electrochemical biosensing, Chemistry, QD1-999
Abstract

Tuberculosis (TB) remains a leading cause of death globally, especially in underdeveloped nations. The main impediment to TB eradication is a lack of efficient diagnostic tools for disease diagnosis. In this work, label free and ultrasensitive electrochemical DNA biosensor for detecting Mycobacterium tuberculosis has been developed based on the electrodeposition of gold nanoparticles on the surface of carbon screen-printed carbon electrode (Zensors) for signal amplification. Particularly, screen-printed electrodes were modified by electrochemical deposition of Au to enhance the conductivity and facilitate the immobilization of ssDNA probes via Au-S bonds. The electrochemically modified SPEs were characterized using Scanning electron microscopy/Energy Dispersive X-Ray Analysis (SEM/EDX) and X-Ray Diffraction (XRD). Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques were used to investigate the DNA hybridization between single-stranded (ssDNA) probe and target DNA (tDNA). Under the ideal conditions, DPV exhibited a correlation coefficient R2 = 0.97, when analyzed with different tDNA concentrations. The proposed DNA biosensor exhibits a good detection range from 2 to 10 nm with a low detection limit of 1.91 nm, as well as high selectivity that, under ideal conditions, distinguishes non-complementary DNA from perfectly matched tDNA. By eliminating the need for DNA purification, this work paves the path for creating disposable biosensors capable of detecting DNA from raw sputum samples.

Published
2022
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3. Induction of secondary metabolites on nanoparticles stress in callus culture of Artemisia annua L.

Author

K. Fatima, S. R. Abbas, M. Zia, S. M. Sabir, R. T. Khan, A. A. Khan, Z. Hassan, and R. Zaman

Subjects
nanoparticles, Artemisia annua L., callus, HPLC, antioxidant activity, total reducing power, Science, Biology (General), QH301-705.5, Zoology, QL1-991, Botany, QK1-989
Abstract

Abstract Nanoparticles are known bio elicitors in plant biotechnology. Different concentrations of ZnO, CuO and CoO nanoparticles were used for the enhanced accumulation of secondary metabolites and antioxidant activities in the callus derived from root, shoot and leaf of Artemisia annua L. Biomass of callus was somehow affected on high concentrations of Nps. Phenolic content was observed maximum (60µg) in shoot callus at 0.1mg/l of CuONps. Total antioxidant activity was observed maximum (33µg) in root callus at 0.1mg/l of ZnOnps. Total reducing power maximum (33µg) was observed in root callus at concentration of 0.05 mg/l of CoONps. Maximum radical scavenging activity was observed in shoot callus at 0.05mg/l of ZnONps. Rutin gallic acid and caffic acid were also determined in most of the samples by HPLC. The study concludes that different Nps have positive effect on the induction of secondary metabolites in A.annua plant.

Published
2020
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4. Study of resveratrol against bone loss by using in-silico and in-vitro methods

Author

S. R. Abbas, R. T. Khan, S. Shafique, S. Mumtaz, A. A. Khan, A. M. Khan, Z. Hassan, S. A. Hussain, S. Abbas, M. R. Abbas, A. Batool, and M. A. Safder

Subjects
resveratrol, protein docking, in-vitro, receptor activator of nuclear factor-kappa-Β ligand [RANKL], fluorescence microscopy, Science, Biology (General), QH301-705.5, Zoology, QL1-991, Botany, QK1-989
Abstract

Abstract By applying the in-silico method, resveratrol was docked on those proteins which are responsible for bone loss. The Molecular docking data between the resveratrol and Receptor activator of nuclear factor-kappa-Β ligand [RANKL] receptors proved that resveratrol binds tightly to the receptors, showed the highest binding affinities of −6.9, −7.6, −7.1, −6.9, −6.7, and −7.1 kcal/mol. According to in-vitro data, Resveratrol reduced the osteoclasts after treating Marrow-Derived Macrophages [BMM] with Macrophage colony-stimulating factor [MCSF] 20ng / ml and RANKL 50ng / ml, with different concentrations of resveratrol (2.5, 10 μg / ml) For 7 days, the cells were treated with MCSF (20 ng / ml) and RANKL (40 ng / ml) together with concentrated trimethyl ether and resveratrol (2.5, 10 μg / ml) within 12 hours. Which, not affect cell survival. After fixing osteoclast cells with formaldehyde fixative on glass coverslip followed by incubation with 0.1% Triton X-100 in PBS for 5 min and after that stain with rhodamine phalloidin staining for actin and Hoechst for nuclei. Fluorescence microscopy was performed to see the distribution of filaments actin [F.actin]. Finally, resveratrol reduced the actin ring formation. Resveratrol is the best bioactive compound for drug preparation against bone loss.

Published
2021
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5. The inhibitory effect of Cannabis Sativa L. and Morus nigra L. against lipid peroxidation in goat liver and brain homogenates

Author

S. A. Hussain, S. R. Abbas, S. M. Sabir, R. T. Khan, S. Ali, M. A. Nafees, S. W. Khan, A. Hussain, Q. Abbas, M. Ali, and S. A. E. Bukhari

Subjects
Cannabis sativa, Morus nigra, antioxidant activity, lipid peroxidation, ABTS assay, liver and brain homogenates, Science, Biology (General), QH301-705.5, Zoology, QL1-991, Botany, QK1-989
Abstract

Abstract The present study was aimed to evaluate the antioxidant potential and inhibitory effect ofCannabis sativa and Morus nigra against lipid peroxidation in goat brain and liver homogenates. The formation of free radicals, highly reactive oxygen species (ROS) and reactive nitrogen species (RNS) is a normal metabolic process for cellular signaling and countering the antigens. However, they may cause serious damage if they produced at amplified tolls. In addition, metabolic disorders also serve as sources of these reactive species. Although the issue can be addressed through supplements and other phytochemicals. In this study, two plant species were evaluated for their biological potential by employing a spectrum of antioxidant assays. The antioxidant activity was performed by lipid peroxidation assay. The water extract prepared from leaves of Cannabis sativa and Morus nigra showed significant (P

Published
2021
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6. Estudo do resveratrol contra a perda óssea usando métodos in silico e in vitro

Author

S. R. Abbas, R. T. Khan, S. Shafique, S. Mumtaz, A. A. Khan, A. M. Khan, Z. Hassan, S. A. Hussain, S. Abbas, M. R. Abbas, A. Batool, and M. A. Safder

Subjects
QH301-705.5, Science, microscopia de fluorescência, RANK Ligand, receptor activator of nuclear factor-kappa-Β ligand [RANKL], Botany, Osteoclasts, Cell Differentiation, resveratrol, in-vitro, fluorescence microscopy, protein docking, Molecular Docking Simulation, docking de proteína, QL1-991, QK1-989, ligante do receptor ativador do fator nuclear kappa-Β [RANKL], Biology (General), General Agricultural and Biological Sciences, Zoology
Abstract

By applying the in-silico method, resveratrol was docked on those proteins which are responsible for bone loss. The Molecular docking data between the resveratrol and Receptor activator of nuclear factor-kappa-Β ligand [RANKL] receptors proved that resveratrol binds tightly to the receptors, showed the highest binding affinities of −6.9, −7.6, −7.1, −6.9, −6.7, and −7.1 kcal/mol. According to in-vitro data, Resveratrol reduced the osteoclasts after treating Marrow-Derived Macrophages [BMM] with Macrophage colony-stimulating factor [MCSF] 20ng / ml and RANKL 50ng / ml, with different concentrations of resveratrol (2.5, 10 μg / ml) For 7 days, the cells were treated with MCSF (20 ng / ml) and RANKL (40 ng / ml) together with concentrated trimethyl ether and resveratrol (2.5, 10 μg / ml) within 12 hours. Which, not affect cell survival. After fixing osteoclast cells with formaldehyde fixative on glass coverslip followed by incubation with 0.1% Triton X-100 in PBS for 5 min and after that stain with rhodamine phalloidin staining for actin and Hoechst for nuclei. Fluorescence microscopy was performed to see the distribution of filaments actin [F.actin]. Finally, resveratrol reduced the actin ring formation. Resveratrol is the best bioactive compound for drug preparation against bone loss. Resumo Com a aplicação do método in-silico, o resveratrol foi ancorado nas proteínas responsáveis pela perda óssea. Os dados de docking molecular entre o resveratrol e o ligante do receptor ativador do fator nuclear kappa-Β [Receptor Activator of Nuclear Factor kappa-B Ligant (RANKL)] provaram que o resveratrol se liga fortemente aos receptores, mostraram as afinidades de ligação mais altas de −6,9, −7,6, −7,1, −6,9, - 6,7 e -7,1 kcal / mol. De acordo com dados in-vitro, o resveratrol reduziu os osteoclastos após o tratamento de macrófagos derivados da medula óssea [Bone Marrow-derived Macrophage (BMM)] com fator estimulador de colônias de macrófagos [Macrophage Colony-Stimulating Factor (MCSF)] 20ng / ml e RANKL 50ng / ml, com diferentes concentrações de resveratrol (2,5, 10 μg / ml). Durante sete dias, as células foram tratadas com MCSF (20 ng / ml) e RANKL (40 ng / ml) juntamente com éter trimetílico concentrado e resveratrol (2,5, 10 μg / ml) em 12 horas, processo que não afeta a sobrevivência celular. Após a fixação de células de osteoclastos com fixador de formaldeído em lamela de vidro seguido de incubação com 0,1% Triton X-100 em PBS por 5 min, foi realizado posteriormente o procedimento para corar com rodamina faloidina a actina e Hoechst os núcleos. A microscopia de fluorescência foi realizada para ver a distribuição dos filamentos de actina [F.actina]. Finalmente, o resveratrol reduziu a formação do anel de actina. O resveratrol é o melhor composto bioativo para o preparo de medicamentos contra a perda óssea.

Published
2023

7. Induction of secondary metabolites on nanoparticles stress in callus culture of Artemisia annua L

Author

Rehmat Zaman, Azhar Abbas Khan, S R Abbas, Syed Mubashar Sabir, Muhammad Zia, K Fatima, Rizwan Taj Khan, and Zeshan Hassan

Subjects
Antioxidant, QH301-705.5, medicine.medical_treatment, Science, Artemisia annua, antioxidant activity, 02 engineering and technology, Biology, High-performance liquid chromatography, Artemisiaannua L, nanopartículas, Antioxidants, 030308 mycology & parasitology, potência redutora total, 03 medical and health sciences, chemistry.chemical_compound, Rutin, Phenols, callus, medicine, atividade antioxidante, total reducing power, Gallic acid, Biology (General), Artemisia annua L, 0303 health sciences, fungi, Botany, food and beverages, calo, 021001 nanoscience & nanotechnology, biology.organism_classification, Plant Leaves, Horticulture, chemistry, QL1-991, Callus, QK1-989, Shoot, Nanoparticles, nanoparticles, HPLC, 0210 nano-technology, General Agricultural and Biological Sciences, Zoology
Abstract

Nanoparticles are known bio elicitors in plant biotechnology. Different concentrations of ZnO, CuO and CoO nanoparticles were used for the enhanced accumulation of secondary metabolites and antioxidant activities in the callus derived from root, shoot and leaf of Artemisia annua L. Biomass of callus was somehow affected on high concentrations of Nps. Phenolic content was observed maximum (60µg) in shoot callus at 0.1mg/l of CuONps. Total antioxidant activity was observed maximum (33µg) in root callus at 0.1mg/l of ZnOnps. Total reducing power maximum (33µg) was observed in root callus at concentration of 0.05 mg/l of CoONps. Maximum radical scavenging activity was observed in shoot callus at 0.05mg/l of ZnONps. Rutin gallic acid and caffic acid were also determined in most of the samples by HPLC. The study concludes that different Nps have positive effect on the induction of secondary metabolites in A.annua plant. Resumo Nanopartículas são bio-elicitores conhecidos em biotecnologia de plantas. Diferentes concentrações de nanopartículas de ZnO, CuO e CoO foram usadas para o acúmulo aumentado de metabólitos secundários e atividades antioxidantes no calo derivado da raiz, parte aérea e folha de Artemisiaannua L. A biomassa do calo foi de alguma forma afetada em altas concentrações de Nps. O conteúdo fenólico foi observado no máximo (60 µg) no calo da parte aérea a 0,1 mg / l de CuONps. A atividade antioxidante total foi observada no máximo (33µg) no calo radicular a 0,1mg / l de ZnOnps. O poder de redução total máximo (33µg) foi observado no calo radicular na concentração de 0,05 mg / l de CoONps. Atividade máxima de eliminação de radicais foi observada no calo da parte aérea a 0,05mg / l de ZnONps. O ácido rutina-gálico e o ácido caffic também foram determinados na maioria das amostras por HPLC. O estudo conclui que diferentes Nps têm efeito positivo na indução de metabólitos secundários na planta de A.annua.

Published
2020

8. HAEMODIALYSIS TECHNIQUES AND ADEQUACY 2

Author

P. Chamney, U. Moissl, P. Wabel, C. Amato, S. Stuard, M. Menzer, C. Vollmeier, G. Williams, R. Shrivastava, J. Chess, E. Catling, C. Brown, E. Baker, R. Ashcroft, A. Mikhail, L. Djukanovic, Z. Djuric, V. Knezevic, T. Lazarevic, S. Ljubenovic, R. Markovic, V. Rabrenovic, J. Marinkovic, N. Dimkovic, L. Lebourg, C. Ridel, H. De Preneuf, F. Le Roy, T. Petitclerc, M. Wester, F. Simonis, J. P. Kooman, W. H. Boer, K. G. F. Gerritsen, J. A. Joles, K.-i. Yamamoto, K. Eguchi, S. Hirakawa, J. Murakami, T. Akiba, M. Mineshima, D. Stamopoulos, N. Mpakirtzi, A. Lavranos, M. Panagiotou, D. Barbarousi, C. Matsouka, E. Grapsa, S. R. Abbas, F. Zhu, G. A. Kaysen, P. Kotanko, N. W. Levin, A. Vasilevsky, G. Konoplev, O. Stepanova, A. Rubinsky, A. Zemchenkov, R. Gerasimchuk, A. Frorip, T. Abe, K.-I. Yamamoto, I. Ishimori, M. Kusztal, T. Go Biowski, K. Letachowicz, P. Koni Ski, G. Witkowski, P. Pozna Ski, W. Weyde, M. Klinger, M. Ito, S. Ito, M. Suzuki, I. Masakane, D. Navarro, C. Goncalves, A. C. Ferreira, C. Jorge, C. Gil, I. Aires, P. Matias, M. Mendes, A. Azevedo, F. Gomes, A. Ferreira, C. Perazzini, L. Scutiero, L. Brighenti, A. Surace, D. Steckiph, P. Rovatti, S. Severi, J. Soltysiak, A. Warzywoda, A. Musielak, D. Ostalska-Nowicka, J. Zachwieja, T. Goeksel, H. Garnier, M. Ritzerfeld, H. Mann, F. Babinet, B. Allard, V. Todorova, C. Hamont, R. Begri, M. Dekker, M. Taks, C. Konings, V. Scharnhorst, J. Borawski, J. Gozdzikiewicz-Lapinska, B. Naumnik, C. A. Lodi, E. Grandi, E. Mancini, A. Santoro, L. Sereni, M. Caiazzo, L. Corazza, M. Atti, G. Palladino, K. Sakurai, T. Saito, H. Hosoya, F. Yamauchi, T. Kurihara, Y. Tanibayashi, N. Ikebe, M. Antonic, J. Gubensek, A. Drozg, E. Vannier, E. Mattio, A. Ragon, P. Brunet, W. Klimm, K. Pleskacz, B. Pietrzak, S. Niemczyk, J. K. Leypoldt, A. Bernardo, M. Muller, T. C. Marbury, B. F. Culleton, A. A. Zeraati, R. Hekmat, H. R. Reyhani, F. Sharifipoor, P. Bolasco, I. Sitzia, A. Monni, M. C. Mereu, A. M. Pinna, F. Logias, T. Ghisu, M. Passaghe, L. Gazzanelli, M. Ganadu, A. Piras, M. Cossu, B. Contu, S. Palleschi, B. Rossi, P. M. Ghezzi, S. Kron, D. Schneditz, T. Leimbach, S. Aign, J. Kron, A. Seker Kockara, M. Kayatas, C. Huzmeli, F. Candan, M. B. Yilmaz, B. A. Ahmed, C. N. Bejosano, S. A. Samra Abouchacra, S. Z. Al Falahi, K. M. Abdul Moniem, H. Dastoor, S. Kim, J. Oh, Y. Sin, J. Kim, and J. Lee

Subjects
03 medical and health sciences, Transplantation, medicine.medical_specialty, 0302 clinical medicine, Nephrology, business.industry, 030232 urology & nephrology, Medicine, 030204 cardiovascular system & hematology, business, Intensive care medicine
Published
2014

9. PERITONEAL DIALYSIS 2

Author

C. A. Vlahu, M. De Graaff, D. G. Struijk, R. T. Krediet, H.-S. Shin, E.-S. Ryu, H.-S. Choi, D.-R. Ryu, K.-B. Choi, D.-H. Kang, E. Sanchez-Alvarez, C. Rodriguez-Suarez, J. A. Galvan-.Hernandez, Y. L. Kim, Y. K. Kee, M. J. Lee, H. J. Oh, J. T. Park, S. H. Han, T.-H. Yoo, S.-W. Kang, F. Zhu, S. R. Abbas, R. Bologa, B. Lanto, P. Kotanko, A. Parikova, W. Smit, M. Rroji ( Molla), S. Seferi, M. Cafka, N. Thereska, C.-C. Huang, I.-K. Wang, Y.-T. Shiao, L. Teixeira, I. Sousa, A. Rodrigues, D. Mendonca, A. Ueda, M. Iwase, T. Usui, A. Hirayama, K. Nagai, C. Saito, K. Yamagata, V. La Milia, G. Pontoriero, F. Locatelli, S. M. Kim, T. Y. Kim, J. E. Lee, D. Teta, M.-P. Guillodo, A. Kolko-Labadens, C. Lasseur, M. Levannier, M. Panaye, D. Fouque, C. HAMADA, K. Hara, S. H. Kang, K. H. Cho, J. W. Park, K. W. Yoon, J. Y. Do, I. Dogan, B. Biro Dr, G. Zakar Dr, Z. Foldine, S. Staudt, A. R. Martins, R. Vizinho, P. Q. Branco, M. A. Gaspar, J. D. Barata, D. Sikorska, P. Klysz, B. Posnik, E. Baum, K. Hoppe, K. Schwermer, M. Wanic-Kossowska, D. Frankiewicz, K. Pawlaczyk, B. Lindholm, A. Oko, M. Busuioc, P. Trolliet, A. Guerraoui, A. Caillette-Beaudoin, P. Hallonet, J.-O. Yang, M. Gursu, D. Topcuoglu, L. K. Koc, L. Yucel, A. Sumnu, E. Cebeci, B. Doner, O. Ozkan, A. Behlul, L. Koc, S. Ozturk, R. Kazancioglu, A. I. I. Casas Parra, M. T. T. Gonzalez, D. A. Sandoval, G. C. Carlota, J. M. M. Grinyo, C.-H. Tseng, C.-T. Chao, C.-J. Yen, C.-K. Chiang, K.-Y. Hung, J.-W. Huang, J. S. Al Wakeel, M. Al Ghonaim, A. Al Suwaida, A. Al Harbi, Z. Makoshi, S. Abdullah, Y. Matsushita, N. Basic-Jukic, D. Coen-Herak, Z. Martinovic, M. Radi -Antoli, P. Kes, T.-J. Wu, J.-S. Chen, S.-H. Lin, J.-C. Shiang, C.-C. Wu, D. Munteanu, M. Gemene, G. Mircescu, S. Opatrna, A. Popperlova, V. Tesar, I. Rychlik, O. Viklicky, K. Jin, B.-S. Park, H. J. Jeong, Y.-W. Kim, S. Hogas, L. Voroneanu, M. Onofriescu, I. Nistor, M. Apetrii, D. Siriopol, M. Cujba, M. Hogas, and A. Covic

Subjects
Transplantation, medicine.medical_specialty, Nephrology, business.industry, medicine.medical_treatment, Urology, Medicine, business, Peritoneal dialysis
Published
2014

10. Nutrition, inflammation and oxidative stress - CKD 1-5

Author

O. Susla, H. S. Shin, Y. S. Jung, H. Rim, T. Speer, F. O. Owala, M. Razawi, E. Holy, B. Ferdinand, F. Danilo, T. F. Luscher, F. C. Tanner, A. Markaki, J. Kyriazis, I. Petrakis, V. Mavroeidi, K. Perakis, G. A. Fragkiadakis, M. Venyhaki, M. Tzanakakis, E. Vardaki, K. Maraki, T. Doskas, E. Daphnis, R. Bregman, B. Vale, C. Lemos, L. Kawakami, M. I. Silva, F. Zhu, G. Kaysen, P. Kotanko, S. R. Abbas, Y. Dou, S. Heymsfield, N. W. Levin, K. Turkmen, H. Kayikcioglu, I. Guney, L. Altintepe, O. Ozbek, H. Z. Tonbul, G. A. Kaysen, L. A. Usvyat, S. Thijssen, R. Mutluay, C. Konca Degertekin, U. Derici, M. I. Yilmaz, F. Akkiyal, S. Gultekin, S. Gonen, S. M. Deger, T. Arinsoy, S. Sindel, M. Hueso, J. Torras, M. Carrera, A. Vidal, E. Navarro, I. Rivas, I. Rama, N. Bolanos, C. Varela, A. Martinez-Castelao, J. M. Grinyo, F. Harving, M. Svensson, E. B. Schmidt, K. A. Jorgensen, J. H. Christensen, J. H. Park, E. H. Koo, H. K. Kim, M. S. Kim, A. J. Cho, J. E. Lee, H. R. Jang, W. Huh, D. J. Kim, Y.-G. Kim, H. Y. Oh, A. Zawiasa, D. Nowak, M. Nowicki, N. Nathalie, G. Griet, S. Eva, V. Raymond, K. P. Ng, S. Stringer, M. Jesky, M. Dutton, C. Ferro, P. Cockwell, T. Jia, T. Gama Axelsson, B. Lindholm, O. Heimburger, P. Barany, P. Stenvinkel, A. R. Qureshi, B. Quiroga, M. Goicoechea, S. Garcia de Vinuesa, U. Verdalles, J. Reque, N. Panizo, D. Arroyo, A. Santos, N. Macias, J. Luno, H. Honda, T. Hirano, M. Ueda, S. Kojima, S. Mashiba, Y. Hayase, T. Michihata, T. Akizawa, O. Gungor, M. Sezis Demirci, F. Kircelli, E. Tatar, E. Hur, S. Sen, H. Toz, A. Basci, E. Ok, V. Sepe, P. Albrizio, M. Gnecchi, E. Cervio, P. Esposito, T. Rampino, C. Libetta, A. Dal Canton, M. S. Faria, S. Ribeiro, G. Silva, H. Nascimento, P. Rocha-Pereira, V. Miranda, E. Vieira, R. Santos, D. Mendonca, A. Quintanilha, E. Costa, L. Belo, A. Santos-Silva, M. Pruijm, L. Hofmann, E. Heuvelin, V. Forni, A. Coristine, M. Stuber, B. Vogt, M. Burnier, M. G. Chiappini, T. Ammann, L. Muzzi, A. Grosso, A. Sabry, V. Bansal, D. Hoppensteadt, W. Jeske, and J. Fareed

Subjects
Transplantation, Nephrology
Published
2012

11. Nutrition, inflammation and oxidative stress - CKD 5D

Author

L. A. Usvyat, J. Raimann, S. Thijssen, F. M. van der Sande, J. Kooman, N. W. Levin, P. Kotanko, G. Von Gersdorff, M. Schaller, I. Bayh, M. Etter, A. Grassmann, A. Guinsburg, M. Lam, D. Marcelli, C. Marelli, L. Scatizzi, A. Tashman, T. Toffelmire, L. Usvyat, F. Van der Sande, Y. Wang, C. Barth, T. Moffitt, F. Hariton, M. Devlin, P. Garrett, M. Hannon-Fletcher, M. Ekramzadeh, Z. Sohrabi, M. Salehi, M. K. Fallahzadeh, M. Ayatollahi, B. Geramizadeh, J. Hassanzadeh, M. M. Sagheb, I. Beberashvili, I. Sinuani, A. Azar, H. Kadoshi, G. Shapiro, L. Feldman, Z. Averbukh, J. Weissgarten, Y. Abe, M. Watanabe, K. Ito, Y. Sasatomi, S. Ogahara, H. Nakashima, T. Saito, S. Witt, R. Kunze, H. J. Guth, H. Skarabis, J. Vienken, P. Nowak, R. Wilk, B. Mamelka, A. Prymont-Przyminska, A. Zwolinska, A. Sarniak, A. Wlodarczyk, J. Rysz, D. Nowak, L. Trajceska, P. Dzekova-Vidimliski, S. Gelev, S. Arsov, A. Sikole, M. Sonikian, A. Dona, I. Skarakis, P. Metaxaki, C. Chiotis, I. Papoutsis, A. Karaitianou, C. Spiliopoulou, F. M. Van der Sande, D. Teta, L. Tappy, N. Theumann, G. Halabi, T. Gauthier, C. Mathieu, S. Tremblay, P. Coti, M. Burnier, A. Zanchi, A. Martinez Vea, C. Cabre, D. Villa, M. Munoz, J. P. Vives, M. Arruche, J. Soler, M. T. Compte, J. Aguilera, M. Romeu, M. Giralt, G. Barril, S. Anaya, C. Vozmediano, A. Celayeta, R. Novillo, V. Bernal, I. Beiret, E. Huarte, J. Martin, H. Santana, G. Torres, F. Sousa, R. Sanchez, A. Lopez-Montes, F. Tornero, J. Uson, M. Pousa, M. Giorgi, B. Rdez Cubillo, R. Malhotra, S. R. Abbas, S. Thjissen, M. Carter, G. von Gersdorff, N. Levin, R. Jens, M. Tepel, E. Katharina, H. Andrea, F. Simone, S. Florian, O. Slusanschi, L. Garneata, R. Moraru, E. Preoteasa, C. Barbulescu, C. Santimbrean, C. Klein, D. Dragomir, G. Mircescu, T. Idorn, F. Knop, J. J. Holst, M. Hornum, B. Feldt-Rasmussen, Y. K. Son, W. S. An, S. E. Kim, K. H. Kim, S. Borrelli, R. Minutolo, L. De Nicola, G. Conte, W. De Simone, B. Zito, P. Guastaferro, F. Nigro, A. Bassi, L. Leone, O. Credendino, R. Genualdo, M. Capuano, G. Iulianiello, M. R. Auricchio, S. Sezer, Z. Bal, E. Tutal, M. Erkmen Uyar, F. N. Ozdemir Acar, S. Ribeiro, M. S. Faria, F. Melo, J. Sereno, I. Freitas, M. Mendonca, H. Nascimento, J. Fernandes, P. Rocha-Pereira, V. Miranda, D. Mendonca, A. Quintanilha, L. Belo, E. Costa, F. Reis, A. Santos-Silva, R. Valtuille, M. E. Casos, and E. A. Fernandez

Subjects
Transplantation, Nephrology, business.industry, Immunology, medicine, Inflammation, medicine.symptom, medicine.disease_cause, business, Oxidative stress
Published
2012

13. Effect of Raphanus raphanistrum on chronic kidney disease induced by ethanol in animal model rats

Author

R. Mushtaq, S. Khwaja, A. Akram, A. Karim, S. R. Abbasi, and K. Baqa

Subjects
Raphanus raphanistrum (radish), reactive oxygen species, alcohol, Science, Biology (General), QH301-705.5, Zoology, QL1-991, Botany, QK1-989
Abstract

Abstract The aim of this study is to investigate the effect of Raphanus raphanistrum (radish) on chronic kidney disease damage by reactive oxygen species or free radicals in animal model rats. Total of 18 rats were used in this study, divided into 3 groups and each group consist of 6 rats. Group 1 control (C), group 2 model (M) and group 3 test (T). Model and test group were treated with alcohol to produce chronic kidney disease by reactive oxygen spices for 9 weeks a dose of 1 ml. After that test group was treated with Raphanus raphanistrum juice for 4 weeks 80mg/kg body weight to determine it effect. Raphanus raphanistrum juice effect on behavior of rats through increases the locomotor activity and anxiety. The serum creatinine and uric acid level were significantly improved in T group. The reactive oxygen enzyme test shows that Super Oxide Dismutase (SOD) and Glutathione Peroxidase (GPx) was increase in T group. The Glutathione S-Transferases (GST) and Catalase (CAT) enzyme level was nearly same in C and T groups. This study concludes that compound 1,1-diphenyl-2-picrylhydrazyl found in Raphanus raphanistrum juice and possess strong antioxidant activity on Chronic kidney disease induce by ethanol through reactive oxygen species. There is need of more researches to determine the use of natural compound to treat different disease.

Published
2022
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14. Extracorporeal dialysis: techniques and adequacy

Author

C. Donadio, A. Kanaki, A. Martin-Gomez, S. Garcia, M. Palacios-Gomez, D. Calia, E. Colombini, F. DI Francesco, S. Ghimenti, M. Onor, D. Tognotti, R. Fuoco, E. Marka-Castro, M. I. Torres Zamora, J. Giron-Mino, M. A. Jaime-Solis, L. M. Arteaga, H. Romero, A. Akonur, K. Leypoldt, M. Asola, B. Culleton, S. Eloot, G. Glorieux, N. Nathalie, R. Vanholder, A. Perez de Jose, U. Verdalles Guzman, S. Abad Esttebanez, A. Vega Martinez, D. Barraca, C. Yuste, L. Bucalo, A. Rincon, J. M. Lopez-Gomez, P. Bataille, P. Celine, A. Raymond, G. Francois, L. Herve, D. Michel, R. Jean Louis, F. Zhu, P. Kotanko, S. Thijssen, N. W. Levin, N. Papamichail, M. Bougiakli, C. Gouva, S. Antoniou, S. Gianitsi, A. Vlachopanou, S. Chachalos, K. Naka, D. Kaarsavvidou, K. Katopodis, L. Michalis, K. Sasaki, K. Yasuda, M. Yamato, A. Surace, P. Rovatti, D. Steckiph, R. Bandini, S. Severi, A. Dellacasa Bellingegni, A. Santoro, M. Arias, A. Sentis, N. Perez, N. Fontsere, M. Vera, N. Rodriguez, C. Arcal, N. Ortega, F. Uriza, A. Cases, F. Maduell, S. R. Abbas, P. Georgianos, P. Sarafidis, P. Nikolaidis, A. Lasaridis, A. Ahmed, H. Kaoutar, B. Mohammed, O. Zouhir, P. Balter, N. Ginsberg, P. Taylor, T. Sullivan, L. A. Usvyat, P. Zabetakis, U. Moissl, M. Ferrario, F. Garzotto, P. Wabel, D. Cruz, C. Tetta, M. G. Signorini, S. Cerutti, A. Brendolan, C. Ronco, J. Heaf, M. Axelsen, R. S. Pedersen, H. Amine, Z. Oualim, A. L. Ammirati, N. K. Guimaraes de Souza, T. Nemoto Matsui, M. Luiz Vieira, W. A. Alves de Oliveira, C. H. Fischer, F. Dias Carneiro, I. J. Iizuka, M. Aparecida de Souza, A. C. Mallet, M. C. Cruz Andreoli, B. F. Cardoso Dos Santos, L. Rosales, Y. Dou, M. Carter, A. Testa, L. Sottini, B. Giacon, E. Prati, C. Loschiavo, M. Brognoli, C. Marseglia, A. Tommasi, L. Sereni, G. Palladino, S. Bove, G. Bosticardo, E. Schillaci, P. Detoma, R. Bergia, J. W. Park, S. J. Moon, H. Y. Choi, S. K. Ha, H.-C. Park, Y. Liao, L. Zhang, P. Fu, H. Igarashi, N. Suzuki, S. Esashi, I. Masakane, V. Panichi, G. De Ferrari, S. Saffiotti, A. Sidoti, M. Biagioli, S. Bianchi, P. Imperiali, C. Gabrielli, P. Conti, P. Patrone, G. Rombola, V. Falqui, C. Mura, A. Icardi, A. Rosati, F. Santori, A. Mannarino, A. Bertucci, J. Jeong, O. K. Kim, N. H. Kim, M. Bots, C. Den Hoedt, M. P. Grooteman, N. C. Van der Weerd, A. H. A. Mazairac, R. Levesque, P. M. Ter Wee, M. J. Nube, P. Blankestijn, M. A. Van den Dorpel, Y. Park, J. Jeon, N. Tessitore, V. Bedogna, D. Girelli, L. Corazza, P. Jacky, Q. Guillaume, B. Julien, W. Marcinkowski, M. Drozdz, A. Milkowski, T. Rydzynska, T. Prystacki, R. August, E. Benedyk-Lorens, K. Bladek, J. Cina, G. Janiszewska, A. Kaczmarek, T. Lewinska, M. Mendel, M. Paszkot, E. Trafidlo, M. Trzciniecka-Kloczkowska, A. Vasilevsky, G. Konoplev, O. Lopatenko, A. Komashnya, K. Visnevsky, R. Gerasimchuk, I. Neivelt, A. Frorip, M. Vostry, J. Racek, D. Rajdl, J. Eiselt, L. Malanova, U. Pechter, A. Selart, M. Ots-Rosenberg, D. H. Krieter, S. Seidel, K. Merget, H.-D. Lemke, C. Wanner, B. Canaud, A. Rodriguez, A. Morgenroth, K. Von Appen, G.-P. Dragoun, R. Fluck, D. Fouque, R. Lockridge, Y. Motomiya, Y. Uji, T. Hiramatsu, Y. Ando, M. Furuta, T. Kuragano, A. Kida, M. Yahiro, Y. Otaki, Y. Hasuike, H. Nonoguchi, T. Nakanishi, M. Sain, V. Kovacic, D. Ljutic, J. Radic, I. Jelicic, S. F. Yalin, S. Trabulus, A. S. Yalin, M. R. Altiparmak, K. Serdengecti, A. Ohtsuka, K. Fukami, K. Ishikawa, R. Ando, Y. Kaida, T. Adachi, K. Sugi, S. Okuda, O. B. Nesterova, E. D. Suglobova, R. V. Golubev, A. N. Vasiliev, V. A. Lazeba, A. V. Smirnov, K. Arita, E. Kihara, K. Maeda, H. Oda, S. Doi, T. Masaki, S. Hidaka, K. Ishioka, M. Oka, H. Moriya, T. Ohtake, S. Nomura, S. Kobayashi, S. Wagner, A. Gmerek, J. Wagner, V. Wizemann, N. Eftimovska - Otovic, K. Spaseska-Gjurovska, S. Bogdanovska, E. Babalj - Banskolieva, M. Milovanceva, R. Grozdanovski, A. Pisani, E. Riccio, A. Mancini, P. Ambuhl, S. Astrid, P. Ivana, H. Martin, K. Thomas, R. Hans-Rudolf, A. Daniel, K. Denes, M. Marco, R. P. Wuthrich, S. Andreas, S. Andrulli, P. Altieri, G. Sau, P. Bolasco, L. A. Pedrini, C. Basile, S. David, M. Feriani, P. E. Nebiolo, R. Ferrara, D. Casu, F. Logias, R. Tarchini, F. Cadinu, M. Passaghe, G. Fundoni, G. Villa, B. R. DI Iorio, C. Zoccali, F. Locatelli, M. Hamamoto, D.-Y. Lee, B. Kim, K. H. Moon, Z. LI, P. Ahrenholz, R. E. Winkler, G. Waitz, H. Wolf, G. Grundstrom, M. Alquist, M. Holmquist, A. Christensson, P. Bjork, M. Abdgawad, L. Ekholm, M. Segelmark, C. Corsi, J. De Bie, E. Mambelli, D. Mortara, D. Arroyo, N. Panizo, B. Quiroga, J. Reque, R. Melero, M. Rodriguez-Ferrero, P. Rodriguez-Benitez, F. Anaya, J. Luno, A. Ragon, A. James, P. Brunet, S. Ribeiro, M. S. Faria, S. Rocha, S. Rodrigues, C. Catarino, F. Reis, H. Nascimento, J. Fernandes, V. Miranda, A. Quintanilha, L. Belo, E. Costa, A. Santos-Silva, J. Arund, R. Tanner, I. Fridolin, M. Luman, C. Clajus, J. T. Kielstein, H. Haller, P. Libutti, P. Lisi, L. Vernaglione, F. Casucci, N. Losurdo, A. Teutonico, C. Lomonte, C. Krisp, D. A. Wolters, M. Matsuyama, T. Tomo, K. Ishida, K. Matsuyama, T. Nakata, J. Kadota, M. Caiazzo, E. Monari, A. Cuoghi, E. Bellei, S. Bergamini, A. Tomasi, T. Baranger, P. Seniuta, F. Berge, V. Drouillat, C. Frangie, E. Rosier, W. Labonia, A. Lescano, D. Rubio, N. Von der Lippe, J. A. Jorgensen, T. B. Osthus, B. Waldum, I. Os, M. Bossola, E. DI Stasio, M. Antocicco, L. Tazza, I. Griveas, A. Karameris, P. Pasadakis, V. Savica, D. Santoro, S. Saitta, V. Tigano, G. Bellinghieri, S. Gangemi, R. Daniela, I. A. Checherita, A. Ciocalteu, I. A. Vacaroiu, A. Niculae, E. Stefaniak, I. Pietrzak, D. Krupa, L. Garred, E. Mancini, L. Corrazza, M. Atti, B. Afsar, D. Stamopoulos, N. Mpakirtzi, B. Gogola, M. Zeibekis, D. Stivarou, M. Panagiotou, E. Grapsa, O. Vega Vega, D. Barraca Nunez, M. Fernandez-Lucas, A. Gomis, J. L. Teruel, S. Elias, C. Quereda, L. Hignell, S. Humphrey, N. Pacy, and N. Afentakis

Subjects
Transplantation, medicine.medical_specialty, Extracorporeal Dialysis, Nephrology, business.industry, Uremic toxins, Medicine, Identification (biology), business, Intensive care medicine, Microbiology
Published
2011

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