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A liquid chromatography-mass spectrometry method was developed for the simultaneous quantitation of (2R,3R)-Dihydromyricetin and Myricetin in rat plasma samples to facilitate analysis of samples collected after administration of SenDeng-4 medicine. The plasma was subjected to protein precipitation with HCl (containing 10% ascorbic acid) and analyzed using electrospray ionization mass spectrometry in selected ion monitoring mode with Paeoniflorin as an internal standard. Good linearity for the two compounds was observed. The intra- and inter-day precision of analysis was, Author(s): Pu Bai [sup.1] [sup.2] [sup.3], Xin Jia [sup.1] [sup.2] [sup.4], Yu Dong [sup.1] [sup.2], Chula Sa [sup.1], Guoyuan Sun [sup.2] [sup.5], Xiaoling Zhu [sup.2] [sup.5] Author Affiliations: (1) grid.410612.0, [...]

Global warming and human activities are complicating the spatial and temporal relationships between basin hydrologic processes and ecosystem quality (EQ), especially in arid and semi-arid regions. Knowledge of the synergy between hydrological processes and ecosystems in arid and semi-arid zones is an effective measure to achieve ecologically sustainable development. In this study, the inland river basin Ulagai River Basin (URB), a typical arid and semi-arid region in Northern China, was used as the study area; based on an improved hydrological model and remote-sensing and in situ measured data, this URB-focused study analyzed the spatial and temporal characteristics of hydrological process factors, such as precipitation, evapotranspiration (ET), surface runoff, lateral flow, groundwater recharge, and EQ and the synergistic relationships between them. It was found that, barring snowmelt, the hydrological process factors such as precipitation, ET, surface runoff, lateral flow, and groundwater recharge had a rising trend in the URB, since the 20th century. The rate of change was higher in the downstream areas when compared with what it was in the upstream and midstream areas. The multi-year average of EQ in the basin is 53.66, which is at a medium level and has an overall improving trend, accounting for 95.14% of the total area, mainly in the upstream, downstream southern, and downstream northern areas of the basin. The change in relationship between the hydrological process factors and EQ was found to have a highly synergistic effect. Temporally, EQ was consistent with the interannual trends of precipitation, surface runoff, lateral flow, and groundwater recharge. The correlation between the hydrological process factors and EQ was found to be higher than 0.7 during the study period. Spatially, the hydrological process factors had a synergistic relationship with EQ from strong to weak upstream, midstream, and downstream, respectively. In addition, ecosystem improvements were accelerated by government initiatives such as the policy of Returning Grazing Land to Grassland Project, which has played an important role in promoting soil and water conservation and EQ. This study provides theoretical support for understanding the relationship between hydrological processes and ecological evolution in arid and semi-arid regions, and it also provides new ideas for related research. [ABSTRACT FROM AUTHOR]

Objective. Heyi disease, Xila disease, and Badagan disease are three common diseases in Mongolian medicine. The changes in intestinal microbiota may be associated with the occurrence, development, and treatment of these diseases. This study aimed to investigate the effects of herbal treatment on intestinal microbiota and serum metabolites in rats with these three diseases. Methods. Firstly, Heyi, Xila, and Badagan disease model rats were established by environmental, diet, and drug intervention. Then, 16S rRNA gene sequencing and metabolomics analysis were used to analyze the changes in intestinal microbiota and serum metabolites after treatment. PICRUSt analysis was applied to predict the potential functions of intestinal microbiota, and OPLS-DA multivariate model was applied to screen differential serum metabolites. Results. 16S rRNA gene sequencing showed that herbal treatment significantly increased the species diversity and changed the composition of intestinal microbiota in Heyi disease and Xila disease rats. After treatment, there were 10, 9, and 3 bacterial biomarkers that were increased in Heyi, Xila, and Badagan disease rats, respectively. In the Heyi disease model, treatment resulted in 45 differential serum metabolites, involving 4 pathways. In the Badagan disease model, treatment resulted in 62 differential serum metabolites, involving 4 pathways. However, there was no significant difference in serum metabolites between TreatB and ConB in the Xila disease model. Conclusions. Herbal treatment significantly changed the intestinal microbiota and serum metabolites of rats with three Mongolian medicine diseases. [ABSTRACT FROM AUTHOR]

Background. Mongolian medicine is a systematic theoretical system, which is based on the balance among Heyi, Xila, and Badagan. However, the underlying mechanisms remain unclear. This study aimed to explore the characteristics of intestinal microbiota and metabolites in different rat models of Mongolian medicine. Methods. After establishing rat models of Heyi, Xila, and Badagan, we integrated 16S rRNA gene sequencing and metabolomics. Results. Heyi, Xila, and Badagan rats had significantly altered intestinal microbial composition compared with rats in the MCK group. They showed 11, 18, and 8 significantly differential bacterial biomarkers and 22, 11, and 15 differential metabolites, respectively. The glucosinolate biosynthesis pathway was enriched only in Heyi rats; the biosynthesis of phenylpropanoids pathway and phenylpropanoid biosynthesis pathway were enriched only in Xila rats; the isoflavonoid biosynthesis pathway, the glycine, serine, and threonine metabolism pathway, and the arginine and proline metabolism pathway were enriched only in Badagan rats. Conclusions. The intestinal microbiota, metabolites, and metabolic pathways significantly differed among Heyi, Xila, and Badagan rats compared with control group rats. [ABSTRACT FROM AUTHOR]