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1. Development of a resonant laser ionization gas cell for high-energy, short-lived nuclei

Author

Sonoda, T., Wada, M., Tomita, H., Sakamoto, C., Takatsuka, T., Furukawa, T., Iimura, H., Ito, Y., Kubo, T., Matsuo, Y., Mita, H., Naimi, S., Nakamura, S., Noto, T., Schury, P., Shinozuka, T., Wakui, T., Miyatake, H., Jeong, S., Ishiyama, H., Watanabe, Y. X., Hirayama, Y., Okada, K., and Takamine, A.

Subjects
Physics - Instrumentation and Detectors, Nuclear Experiment
Abstract

A new laser ion source configuration based on resonant photoionization in a gas cell has been developed at RIBF RIKEN. This system is intended for the future PArasitic RI-beam production by Laser Ion-Source (PALIS) project which will be installed at RIKEN's fragment separator, BigRIPS. A novel implementation of differential pumping, in combination with a sextupole ion beam guide (SPIG), has been developed. A few small scroll pumps create a pressure difference from 1000 hPa - 10^-3 Pa within a geometry drastically miniaturized compared to conventional systems. This system can utilize a large exit hole for fast evacuation times, minimizing the decay loss for short-lived nuclei during extraction from a buffer gas cell, while sufficient gas cell pressure is maintained for stopping high energy RI-beams. In spite of the motion in a dense pressure gradient, the photo-ionized ions inside the gas cell are ejected with an assisting force gas jet and successfully transported to a high-vacuum region via SPIG followed by a quadrupole mass separator. Observed behaviors agree with the results of gas flow and Monte Carlo simulations.

Published
2012
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4. Mixed coating mortars using tire rubber: performance in the face of water.

Author

Sakamoto, C. A., Pagoto, L. M., Akasaki, J. L., Pinheiro Melges, J. L., and Fabiano Fioriti, C.

Subjects
*MORTARS (Ordnance), *GRAIN size, *COMPOSITE materials, *CONSTRUCTION project management, *REINFORCED concrete, *COMPRESSIVE strength
Abstract

This paper evaluated the behavioural performance to coating mortar water with rubberized tyres. #0.60 mm and #1.19 mm grain sizes have been used with the incorporation of 10 and 20% (in volume), replacing the small units. The methodology has elapsed with capillary and drying tests; humidification and drying procedures with mass control, infrared thermography and measurement of humidity. The results indicated that in capillarity and drying the trace A20F (#0.60 mm particle size and incorporation of 20%) had have a low absorption rate and helped in the process of water disposal. In the humidification and drying process, both active as well as passive, the rubber possibly blocks the pores of mass, for the grain traits size #0.60 mm (A10F and primarily A20F) have proven easy to lose water, suggesting that the majority of water absorbed would be concentrated on the surface part of the composite. [ABSTRACT FROM AUTHOR]

Published
2023
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5. Assessing the Relationship of Patient Reported Outcome Measures With Functional Status in Dysferlinopathy: A Rasch Analysis Approach

Author

Mayhew, A. G., James, M. K., Moore, U., Sutherland, H., Jacobs, M., Feng, J., Lowes, L. P., Alfano, L. N., Muni Lofra, R., Rufibach, L. E., Rose, K., Duong, T., Bello, L., Pedrosa-Hernandez, I., Holsten, S., Sakamoto, C., Canal, A., Sanchez-Aguilera Praxedes, N., Thiele, S., Siener, C., Vandevelde, B., Dewolf, B., Maron, E., Gordish-Dressman, H., Hilsden, H., Guglieri, M., Hogrel, J. -Y., Blamire, A. M., Carlier, P. G., Spuler, S., Day, J. W., Jones, K. J., Bharucha-Goebel, D. X., Salort-Campana, E., Pestronk, A., Walter, M. C., Paradas, C., Stojkovic, T., Mori-Yoshimura, M., Bravver, E., Diaz-Manera, J., Pegoraro, E., Mendell, J. R., Jain COS Consortium, Straub, V., Jain Foundation, John Walton Centre Muscular Dystrophy Research Centre, and MRC Centre Neuromuscular Biobank (UK)

Subjects
PROMs, Neurology, quality of life, Neurology (clinical), clinical outcome assessments, dysferlinopathy, limb girdle muscular dystrophy, Function and Dysfunction of the Nervous System
Abstract

Dysferlinopathy is a muscular dystrophy with a highly variable functional disease progression in which the relationship of function to some patient reported outcome measures (PROMs) has not been previously reported. This analysis aims to identify the suitability of PROMs and their association with motor performance.Two-hundred and four patients with dysferlinopathy were identified in the Jain Foundation's Clinical Outcome Study in Dysferlinopathy from 14 sites in 8 countries. All patients completed the following PROMs: Individualized Neuromuscular Quality of Life Questionnaire (INQoL), International Physical Activity Questionnaire (IPAQ), and activity limitations for patients with upper and/or lower limb impairments (ACTIVLIMs). In addition, nonambulant patients completed the Egen Klassifikation Scale (EK). Assessments were conducted annually at baseline, years 1, 2, 3, and 4. Data were also collected on the North Star Assessment for Limb Girdle Type Muscular Dystrophies (NSAD) and Performance of Upper Limb (PUL) at these time points from year 2. Data were analyzed using descriptive statistics and Rasch analysis was conducted on ACTIVLIM, EK, INQoL. For associations, graphs (NSAD with ACTIVLIM, IPAQ and INQoL and EK with PUL) were generated from generalized estimating equations (GEE). The ACTIVLIM appeared robust psychometrically and was strongly associated with the NSAD total score (Pseudo R 2 0.68). The INQoL performed less well and was poorly associated with the NSAD total score (Pseudo R 2 0.18). EK scores were strongly associated with PUL (Pseudo R 2 0.69). IPAQ was poorly associated with NSAD scores (Pseudo R 2 0.09). This study showed that several of the chosen PROMs demonstrated change over time and a good association with functional outcomes. An alternative quality of life measure and method of collecting data on physical activity may need to be selected for assessing dysferlinopathy., The estimated US $4 million needed to fund this study was provided by the Jain Foundation (www.jainfoundation.org). The John Walton Muscular Dystrophy Research Centre is part of the MRC Centre for Neuromuscular Diseases (Grant Number MR/K000608/1).

Published
2022

9. Monocyte chemoattractant protein 1 (MCP-1) released from Helicobacter pylori stimulated gastric epithelial cells induces cyclooxygenase 2 expression and activation in T cells

Author

Futagami, S, Hiratsuka, T, Tatsuguchi, A, Suzuki, K, Kusunoki, M, Shinji, Y, Shinoki, K, Iizumi, T, Akamatsu, T, Nishigaki, H, Wada, K, Miyake, K, Gudis, K, Tsukui, T, and Sakamoto, C

Subjects
Gastric mucosa -- Physiological aspects, Cytokines -- Physiological aspects, Helicobacter infections -- Physiological aspects, Monocytes -- Physiological aspects, Health, Physiological aspects
Abstract

Background and aims: To clarify the interaction between gastric epithelial and mucosal T cells, we examined the role of cytokines released from epithelial cells in response to Helicobacter pylori water [...]

Published
2003

12. Assessment of disease progression in dysferlinopathy: a 1-year cohort study

Author

Moore, U., Jacobs, Marni, James, Meredith K, Mayhew, A. G., Fernandez-Torron, Roberto, Feng, J., Cnaan, A., Eagle, M., Bettinson, K., Rufibach, L. E., Lofra, R. M., Blamire, A. M., Carlier, P. G., Mittal, P., Lowes, L. P., Alfano, L., Rose, K., Duong, T., Berry, K. M., Montiel-Morillo, E., Pedrosa-Hernández, I., Holsten, S., Sanjak, M., Ashida, A., Sakamoto, C., Tateishi, T., Yajima, H., Canal, A., Ollivier, G., Decostre, V., Mendez, J. B., Praxedes, N. S. A., Thiele, S., Siener, C., Shierbecker, J., Florence, J. M., Vandevelde, B., Dewolf, B., Hutchence, M., Gee, R., Prügel, J., Maron, E., Hilsden, Heather, Lochmüller, H., Grieben, U., Spuler, Simone, Rocha, C. T., Day, J. W., Jones, K. J., Bharucha-Goebel, D. X., Salort-Campana, Emmanuelle, Harms, M., Pestronk, Alan, Krause, S., Schreiber-Katz, Olivia, Walter, M. C., Paradas, C., Hogrel, J. Y., Stojkovic, T., Takeda, S., Mori-Yoshimura, M., Bravver, Elena, Sparks, S., Diaz-Manera, Jordi., Bello, Luca, Semplicini, C., Pegoraro, E., Mendell, J. R., Bushby, Kate, Straub, V., Universitat Autònoma de Barcelona, and Jain Foundation

Subjects
0301 basic medicine, medicine.medical_specialty, Dysferlinopathy, business.industry, Wrist, medicine.disease, 3. Good health, Test (assessment), Clinical trial, Manual Muscle Testing, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, medicine.anatomical_structure, Cardiovascular and Metabolic Diseases, Ambulatory, Physical therapy, medicine, Clinical endpoint, Neurology (clinical), business, 030217 neurology & neurosurgery, Cohort study
Abstract

Jain COS Consortium., [Objective] To assess the ability of functional measures to detect disease progression in dysferlinopathy over 6 months and 1 year., [Methods] One hundred ninety-three patients with dysferlinopathy were recruited to the Jain Foundation's International Clinical Outcome Study for Dysferlinopathy. Baseline, 6-month, and 1-year assessments included adapted North Star Ambulatory Assessment (a-NSAA), Motor Function Measure (MFM-20), timed function tests, 6-minute walk test (6MWT), Brooke scale, Jebsen test, manual muscle testing, and hand-held dynamometry. Patients also completed the ACTIVLIM questionnaire. Change in each measure over 6 months and 1 year was calculated and compared between disease severity (ambulant [mild, moderate, or severe based on a-NSAA score] or nonambulant [unable to complete a 10-meter walk]) and clinical diagnosis., [Results] The functional a-NSAA test was the most sensitive to deterioration for ambulant patients overall. The a-NSAA score was the most sensitive test in the mild and moderate groups, while the 6MWT was most sensitive in the severe group. The 10-meter walk test was the only test showing significant change across all ambulant severity groups. In nonambulant patients, the MFM domain 3, wrist flexion strength, and pinch grip were most sensitive. Progression rates did not differ by clinical diagnosis. Power calculations determined that 46 moderately affected patients are required to determine clinical effectiveness for a hypothetical 1-year clinical trial based on the a-NSAA as a clinical endpoint., [Conclusion] Certain functional outcome measures can detect changes over 6 months and 1 year in dysferlinopathy and potentially be useful in monitoring progression in clinical trials., [ClinicalTrials.gov identifier] NCT01676077., The estimated US $4 million needed to fund this study is being provided by the Jain Foundation. The John Walton Centre Muscular Dystrophy Research Centre is part of the MRC Centre for Neuromuscular Diseases (grant MR/K000608/1).

Published
2019

21. A high-level and regulatable production system for recombinant glycoproteins using a human interferon-α promoter-based expression vector

Author

Fukuda S, Murakami T, Fujii M, Sato M, Mori T, Yamamoto K, Kurimoto M, Koide K, Sakamoto C, and Ohta T

Subjects
DNA, Complementary, Genetic Vectors, Hamster, Alpha interferon, law.invention, Transformation, Genetic, law, Cricetinae, Complementary DNA, Tumor Cells, Cultured, Genetics, Animals, Humans, Promoter Regions, Genetic, Erythropoietin, Glycoproteins, Expression vector, biology, Tumor Necrosis Factor-alpha, Chinese hamster ovary cell, Interferon-alpha, General Medicine, biology.organism_classification, Molecular biology, Recombinant Proteins, Sendai virus, Recombinant DNA, Tumor necrosis factor alpha
Abstract

A novel expression vector for human cells, pIFP, which expresses a cloned gene under the control of the human interferon-α-encoding gene ( IFN -α) promoter ( pIFN ) was constructed. As a model of glycoprotein production, a human erythropoietin-encoding cDNA ( EPO ) inserted downstream from pIFN in pIFP was introduced into human B-cell leukemia-derived BALL-1 cells, and EPO-producing cells were established. Upon stimulation with Sendai virus, the cells produced human EPO at high levels. The highest production level and the highest inducibility were 872 IU/ml and 67-fold, respectively. Simultaneously, the transformed cells also produced IFN-α and tumor necrosis factor-α (TNF-α), as the parental BALL-1 cells did. Comparing the amounts of the substances produced, activity of the exogenous pIFN introduced seemed much higher than that of the endogenous one. Further, the transformed cells could be obtained in a large quantity by being applied to the ‘in vivo cell proliferation method (hamster method)’. Human EPO produced by the transformed cells had a molecular mass range of 35 to 42 kDa, similar to that of EPO produced by CHO cells. The processing of EPO seemed to occur properly. The combination of the human pIFN , BALL-1 cells and the hamster method provides us with a useful production system for bioactive glycoproteins of human origin.

Published
1994

26. ANTI-HELMÍNTICA DE NEMATÓDEOS GASTRINTESTINAIS DE OVINOS NA REGIÃO DE UMUARAMA-PR.

Author

BARRAGAN, F. G., LORGA, A. D., CATUSSI, B. L. C., BENEDITO, G. S., NAKATANI, M. T. M., and SAKAMOTO, C. A. M.

Abstract

Copyright of Archives of Veterinary Science is the property of Archives of Veterinary Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2016

27. AVALIAÇÃO ENDOPARASITICIDA DAS MOLÉCULAS MOXIDECTINA E IVERMECTINA CONTRA NEMATÓDEOS.

Author

LORGA, A. D., SOUZA, A. F. P., COLLI, M. H. A., RUIVO, M. A., AMIM, M. B., MARTINEZ, A. C., and SAKAMOTO, C. A. M.

Abstract

Copyright of Archives of Veterinary Science is the property of Archives of Veterinary Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2016

32. Preventive therapy for non-steroidal anti-inflammatory drug-induced ulcers in Japanese patients with rheumatoid arthritis: the current situation and a prospective controlled-study of the preventive effects of lansoprazole or famotidine

Author

Miyake, K., primary, Ueki, N., additional, Suzuki, K., additional, Shinji, Y., additional, Kusunoki, M., additional, Hiratsuka, T., additional, Nishigaki, H., additional, Tatsuguchi, A., additional, Futagami, S., additional, Wada, K., additional, Tsukui, T., additional, Nakajima, A., additional, Yoshino, S., additional, and Sakamoto, C., additional

Published
2005
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37. Testing the iron hypothesis in ecosystems of the equatorial Pacific Ocean

Author

Martin, J.H., Coale, K.H., Johnson, K.S., Fitzwater, S.E., Gordon, R.M., Tanner, S.J., Hunter, C.N., Elrod, V.A., Nowicki, J.L., Coley, T.L., Barber, R.T., Lindley, S., Watson, A.J., Van Scoy, K., Law, C.S., Liddicoat, M.I., Ling, R., Stanton, T., Stockel, J., Collins, C., Anderson, A., Bidigare, R., Ondrusek, M., Latasa, M., Millero, F.J., Lee, K., Yao, W., Zhang, J.Z., Friederich, G., Sakamoto, C., Chavez, F., Buck, K., Kolber, Z., Greene, R., Falkowski, P., Chisholm, S.W., Hoge, F., Swift, R., Yungel, J., Turner, S., Nightingale, P., Hatton, A., Liss, P., Tindale, N.W., Martin, J.H., Coale, K.H., Johnson, K.S., Fitzwater, S.E., Gordon, R.M., Tanner, S.J., Hunter, C.N., Elrod, V.A., Nowicki, J.L., Coley, T.L., Barber, R.T., Lindley, S., Watson, A.J., Van Scoy, K., Law, C.S., Liddicoat, M.I., Ling, R., Stanton, T., Stockel, J., Collins, C., Anderson, A., Bidigare, R., Ondrusek, M., Latasa, M., Millero, F.J., Lee, K., Yao, W., Zhang, J.Z., Friederich, G., Sakamoto, C., Chavez, F., Buck, K., Kolber, Z., Greene, R., Falkowski, P., Chisholm, S.W., Hoge, F., Swift, R., Yungel, J., Turner, S., Nightingale, P., Hatton, A., Liss, P., and Tindale, N.W.

Abstract

The idea that iron might limit phytoplankton growth in large regions of the ocean has been tested by enriching an area of 64 km2 in the open equatorial Pacific Ocean with iron. This resulted in a doubling of plant biomass, a threefold increase in chlorophyll and a fourfold increase in plant production. Similar increases were found in a chlorophyll-rich plume down-stream of the Galapagos Islands, which was naturally enriched in iron. These findings indicate that iron limitation can control rates of phytoplankton productivity and biomass in the ocean.

Published
1994

38. Testing the iron hypothesis in ecosystems of the equatorial Pacific Ocean

Author

Meteorology, Martin, J.H., Coale, K.H., Johnson, K.S., Fitzwater, S.E., Gordon, R.M., Tanner, S.J., Hunter, C.N., Elrod, V.A., Nowicki, J.L., Coley, T.L., Barber, R.T., Lindley, S., Watson, A.J., Van Scoy, K., Law, C.S., Liddicoat, M.I., Ling, R., Stanton, Timothy P., Stockel, J., Collins, C., Anderson, A., Bidigare, R., Ondrusek, M., Latasa, M., Millero, F.J., Lee, K., Yao, W., Zhang, J.Z., Friederich, G., Sakamoto, C., Chavez, F., Buck, K., Kolber, Z., Greene, R., Falkowski, P., Chisholm, S.W., Hoge, F., Swift, R., Yungel, J., Turner, S., Nightingale, P., Hatton, A., Liss, P., Tindale, N.W., Meteorology, Martin, J.H., Coale, K.H., Johnson, K.S., Fitzwater, S.E., Gordon, R.M., Tanner, S.J., Hunter, C.N., Elrod, V.A., Nowicki, J.L., Coley, T.L., Barber, R.T., Lindley, S., Watson, A.J., Van Scoy, K., Law, C.S., Liddicoat, M.I., Ling, R., Stanton, Timothy P., Stockel, J., Collins, C., Anderson, A., Bidigare, R., Ondrusek, M., Latasa, M., Millero, F.J., Lee, K., Yao, W., Zhang, J.Z., Friederich, G., Sakamoto, C., Chavez, F., Buck, K., Kolber, Z., Greene, R., Falkowski, P., Chisholm, S.W., Hoge, F., Swift, R., Yungel, J., Turner, S., Nightingale, P., Hatton, A., Liss, P., and Tindale, N.W.

Abstract

The idea that iron might limit phytoplankton growth in large regions of the ocean has been tested by enriching an area of 64 km in the open equatorial Pacific Ocean with iron. This resulted in a doubling of plant biomass, a threefold increase in chlorophyll and a fourfold increase in plant production. Similar increases were found in a chlorophyll-rich plume down-stream of the Galapagos Islands, which was naturally enriched in iron. These findings indicate that iron limitation can control rates of phytoplankton productivity and biomass in the ocean.

Published
1994

41. Testing the iron hypothesis in ecosystems of the equatorial Pacific Ocean

Author

Martin, J. H., primary, Coale, K. H., additional, Johnson, K. S., additional, Fitzwater, S. E., additional, Gordon, R. M., additional, Tanner, S. J., additional, Hunter, C. N., additional, Elrod, V. A., additional, Nowicki, J. L., additional, Coley, T. L., additional, Barber, R. T., additional, Lindley, S., additional, Watson, A. J., additional, Van Scoy, K., additional, Law, C. S., additional, Liddicoat, M. I., additional, Ling, R., additional, Stanton, T., additional, Stockel, J., additional, Collins, C., additional, Anderson, A., additional, Bidigare, R., additional, Ondrusek, M., additional, Latasa, M., additional, Millero, F. J., additional, Lee, K., additional, Yao, W., additional, Zhang, J. Z., additional, Friederich, G., additional, Sakamoto, C., additional, Chavez, F., additional, Buck, K., additional, Kolber, Z., additional, Greene, R., additional, Falkowski, P., additional, Chisholm, S. W., additional, Hoge, F., additional, Swift, R., additional, Yungel, J., additional, Turner, S., additional, Nightingale, P., additional, Hatton, A., additional, Liss, P., additional, and Tindale, N. W., additional

Published
1994
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45. RESISTÊNCIA MÚLTIPLA DE Rhipicephalus microplus FRENTE AOS ACARICIDAS NA REGIÃO DE UMUARAMA-PR.

Author

ENDO, V. T., NAKATANI, M. T. M., BARRAGAN, F. G., PINTO, A. A., MARTINEZ, A. C., and SAKAMOTO, C. A. M.

Abstract

Copyright of Archives of Veterinary Science is the property of Archives of Veterinary Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2016

49. Expression of heregulin @a, erbB2, and erbB3 and their influences on proliferation of gastric epithelial cells

Author

Noguchi, H., Sakamoto, C., Wada, K., Akamatsu, T., Uchida, T., Tatsuguchi, A., Matsui, H., Fukui, H., Fujimori, T., and Kasuga, M.

Abstract

Background & Aims: Heregulins (HRGs) regulate the proliferation and differentiation of various cell types. However, very little is known about their function in the gastrointestinal tract. The aim of this study was to investigate the role of HRGs on gastrointestinal cells. Methods: We examined the expression of erbB receptors and HRG-@a in human gastric cancer cell lines, rat gastrointestinal epithelial cells, and human gastric fibroblasts by Western blot analysis or reverse-transcription polymerase chain reaction. Receptor phosphorylation and heterodimerization induced by HRG-@a were detected by Western blot analysis. We also evaluated the in vitro effects of HRG-@a on cell proliferation and restitution. Results: Cancer cell lines and rat epithelial cells expressed erbB2 and erbB3, but protein expression of erbB4 was not detected. HRG-@a was detected only in gastric fibroblasts. HRG-@a activated tyrosine phosphorylation of epidermal growth factor receptor (EGFR), erbB2, and erbB3 and induced not only erbB3/erbB2 but also erbB3/EGFR and erbB2/EGFR heterodimer formation in MKN-28 cancer cells. Simultaneous cultivation of MKN-28 cells with gastric fibroblasts resulted in tyrosine phosphorylation of erbB3 in MKN-28 cells. HRG-@a also stimulated proliferation of MKN-28 cells and gastric epithelial cells. Conclusions: The data suggest that HRG-@a may affect epithelial cell proliferation through mesenchymal-epithelial interaction in the gastric mucosa. GASTROENTEROLOGY 1999;117:1119-1127

Published
1999
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50. Somatostatin receptors on rat cerebrocortical membranes. Structural characterization of somatostatin-14 and somatostatin-28 receptors and comparison with pancreatic type receptors.

Author

Sakamoto, C, Nagao, M, Matozaki, T, Nishizaki, H, Konda, Y, and Baba, S

Abstract

Somatostatin binding and cross-linking to its receptors on rat cerebrocortical membranes were characterized with [125I-Tyr1]somatostatin-14 and [125I-Leu8, D-Trp22, Tyr25]somatostatin-28. When [125I-Tyr1]somatostatin-14 was cross-linked to its receptors with the photoreactive cross-linker, N-(5-azido-2-nitrobenzoyloxy)succinimide, the hormone was specifically associated with a Mr = 72,000 protein band in the presence or absence of reducing agents. Affinity labeling of the Mr = 72,000 protein band was decreased with increasing concentrations of unlabeled somatostatin-14 and nonhydrolyzable guanine nucleotide analog, guanyl-5′-yl imidodiphosphate (Gpp(NH)p). Pretreatment of cerebrocortical membranes with islet-activating protein resulted in a decrease in subsequent labeled somatostatin-14 binding and affinity-labeling of the protein and abolished an inhibitory effect of somatostatin-14 on vasoactive intestinal peptide-stimulated increase in adenylate cyclase activity. When the affinity-labeled protein was solubilized with Zwittergent 3-12 and adsorbed to wheat germ agglutinin-agarose, it was eluted by N-acetylglucosamine. [125I-Leu8, D-Trp22, Tyr25]somatostatin-28 cross-linking to cerebrocortical and pancreatic membranes with the same photoreactive agent revealed specifically labeled protein bands of a Mr = 74,000 in cerebrocortical membranes and a Mr = 94,000 in pancreatic membranes, respectively. These results suggest that: 1) somatostatin receptor on cerebrocortical membranes is a monomeric glycoprotein with a Mr = 70,000 binding subunit, coupled to guanine nucleotide regulatory protein, and 2) the Mr = 70,000 protein may be a common receptor for somatostatin-28 and somatostatin-14 and is distinct from a common pancreatic type receptor.

Published
1988
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