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5 results on '"Seng, Jingjing"'

1. EZH2 Contributes To Cisplatin Resistance In Breast Cancer By Epigenetically Suppressing miR-381 Expression

Author

Dou, Dongwei, Ge, Xin, Wang, Xinxing, Xu, Xiaodong, Zhang, Zhe, Seng, Jingjing, Cao, Zhang, Gu, Yuanting, and Han, Mingli

Subjects
inorganic chemicals, breast cancer, miR-381, CDDP, enhancer of zeste homolog 2, macromolecular substances, skin and connective tissue diseases, neoplasms, female genital diseases and pregnancy complications, OncoTargets and Therapy, Original Research
Abstract

Dongwei Dou,* Xin Ge,* Xinxing Wang, Xiaodong Xu, Zhe Zhang, Jingjing Seng, Zhang Cao, Yuanting Gu, Mingli Han Department of Breast Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 475000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Mingli HanDepartment of Breast Surgery, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe East Road, Zhengzhou 450000, Henan Province, People’s Republic of ChinaTel +86-371-66273586Email mlhanzzuniver@126.comBackground: Emerging evidence reveals the vital role of enhancer of zeste homolog 2 (EZH2) in cancer chemoresistance. However, its function and molecular mechanisms in breast cancer chemoresistance remain largely unknown.Methods: Gene expression was evaluated using quantitative real-time PCR (qRT-PCR) and Western blot analysis. The functional roles of EZH2 and miR-381 in breast cancer were explored using cell MTT assay and flow cytometry analysis. The effect of EZH2 on miR-381 expression in transcriptional level was determined using Chromatin immunoprecipitation (ChIP) assay and Luciferase reporter assay.Results: In this study, we found that EZH2 was up-regulated in CDDP-resistant breast cancer tissues and cell lines. Breast cancer patients with high EZH2 expression had a poor prognosis. EZH2 silencing improved the sensitivity of MCF-7/CDDP and MDA-MB-231/CDDP cells towards CDDP. Moreover, EZH2 could epigenetically silence miR-381. miR-381 overexpression could overcome CDDP resistance in CDDP-resistant breast cancer cells. miR-381 knockdown weakened the inductive effect of EZH2 silencing on CDDP sensitivity of MCF-7/CDDP and MDA-MB-231/CDDP cells. Furthermore, EZH2 knockdown facilitated CDDP sensitivity of CDDP-resistant breast cancer cells in vivo.Conclusions: Collectively, EZH2 depletion overcame CDDP resistance of breast cancer through epigenetically silencing miR-381, providing a novel therapeutic target for breast cancer chemoresistance.Keywords: breast cancer, CDDP, enhancer of zeste homolog 2, miR-381

Published
2019

2. EZH2 Contributes To Cisplatin Resistance In Breast Cancer By Epigenetically Suppressing miR-381 Expression

Author

Dou,Dongwei, Ge,Xin, Wang,Xinxing, Xu,Xiaodong, Zhang,Zhe, Seng,Jingjing, Cao,Zhang, Gu,Yuanting, Han,Mingli, Dou,Dongwei, Ge,Xin, Wang,Xinxing, Xu,Xiaodong, Zhang,Zhe, Seng,Jingjing, Cao,Zhang, Gu,Yuanting, and Han,Mingli

Abstract

Dongwei Dou,* Xin Ge,* Xinxing Wang, Xiaodong Xu, Zhe Zhang, Jingjing Seng, Zhang Cao, Yuanting Gu, Mingli Han Department of Breast Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 475000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Mingli HanDepartment of Breast Surgery, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe East Road, Zhengzhou 450000, Henan Province, People’s Republic of ChinaTel +86-371-66273586Email mlhanzzuniver@126.comBackground: Emerging evidence reveals the vital role of enhancer of zeste homolog 2 (EZH2) in cancer chemoresistance. However, its function and molecular mechanisms in breast cancer chemoresistance remain largely unknown.Methods: Gene expression was evaluated using quantitative real-time PCR (qRT-PCR) and Western blot analysis. The functional roles of EZH2 and miR-381 in breast cancer were explored using cell MTT assay and flow cytometry analysis. The effect of EZH2 on miR-381 expression in transcriptional level was determined using Chromatin immunoprecipitation (ChIP) assay and Luciferase reporter assay.Results: In this study, we found that EZH2 was up-regulated in CDDP-resistant breast cancer tissues and cell lines. Breast cancer patients with high EZH2 expression had a poor prognosis. EZH2 silencing improved the sensitivity of MCF-7/CDDP and MDA-MB-231/CDDP cells towards CDDP. Moreover, EZH2 could epigenetically silence miR-381. miR-381 overexpression could overcome CDDP resistance in CDDP-resistant breast cancer cells. miR-381 knockdown weakened the inductive effect of EZH2 silencing on CDDP sensitivity of MCF-7/CDDP and MDA-MB-231/CDDP cells. Furthermore, EZH2 knockdown facilitated CDDP sensitivity of CDDP-resistant breast cancer cells in vivo.Conclusions: Collectively, EZH2 depletion overcame CDDP resistance of breast cancer through epigenetically silencing miR-381, providing a novel therapeutic target for breast cancer chem

Published
2019

5. Diagnosis and surgical treatment of ruptured aneurysm in sinus of Valsalva.

Author

Zhao G, Seng J, Yan B, Wei H, Qiao C, Zhao S, Zhao W, and Zhi X

Subjects
Adolescent, Adult, Child, Female, Humans, Male, Middle Aged, Aortic Aneurysm diagnosis, Aortic Aneurysm surgery, Aortic Rupture diagnosis, Aortic Rupture surgery, Sinus of Valsalva
Abstract

Objective: To evaluate the methods used to diagnose and surgically treat ruptured aneurysm in sinus of Valsalva (RASV)., Methods: Thirty-seven hospitalized patients with ruptured aneurysms in the sinus of Valsalva from September 1981 to April 2001, including 21 cases (56.7%) of RASV associated with ventricular septal defects (VSD) and 11 (29.7%) with aortic valvular prolapse were given surgical interventions. Under hypothermia and extracorporeal circulation, we successfully performed the surgical correction of RASV for all 37 patients VSD repair in 21 patients, aortic valvuloplasty in 6 and aortic valvular replacement in 2., Results: There was no hospital deaths among these patients, although residual shunting occurred in two patients and acute renal failure was found one. Follow-up study of one month to 20 years in the patients undergoing repair of RASV revealed that the mostly individuals treated with operation obtained satisfactory cardiac function., Conclusion: Correct diagnosis of ruptured aneurysm in sinus of Valsalva should be confirmed immediately and surgical correction should be carried out as soon as possible.

Published
2003

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