1. CsIVP functions in vasculature development and downy mildew resistance in cucumber
- Author
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Yan, Shuangshuang, Ning, Kang, Wang, Zhongyi, Liu, Xiaofeng, Zhong, Yanting, Ding, Lian, Zi, Hailing, Cheng, Zhihua, Li, Xuexian, Shan, Hongyan, Lv, Qingyang, Luo, Laixin, Liu, Renyi, Yan, Liying, Zhou, Zhaoyang, Lucas, William John, and Zhang, Xiaolan
- Subjects
Agricultural ,Veterinary and Food Sciences ,Plant Biology ,Biological Sciences ,Crop and Pasture Production ,Stem Cell Research - Nonembryonic - Non-Human ,Genetics ,Biotechnology ,Stem Cell Research ,Basic Helix-Loop-Helix Transcription Factors ,Cucumis sativus ,Disease Resistance ,Gene Expression ,Gene Expression Regulation ,Plant ,Indoleacetic Acids ,Morphogenesis ,Phylogeny ,Plant Diseases ,Plant Proteins ,Plant Vascular Bundle ,Plants ,Genetically Modified ,Protein Binding ,Salicylic Acid ,Signal Transduction ,Agricultural and Veterinary Sciences ,Medical and Health Sciences ,Developmental Biology ,Agricultural ,veterinary and food sciences ,Biological sciences ,Biomedical and clinical sciences - Abstract
Domesticated crops with high yield and quality are frequently susceptible to pathogen attack, whereas enhancement of disease resistance generally compromises crop yield. The underlying mechanisms of how plant development and disease resistance are coordinately programed remain elusive. Here, we showed that the basic Helix-Loop-Helix (bHLH) transcription factor Cucumis sativus Irregular Vasculature Patterning (CsIVP) was highly expressed in cucumber vascular tissues. Knockdown of CsIVP caused severe vasculature disorganization and abnormal organ morphogenesis. CsIVP directly binds to vascular-related regulators YABBY5 (CsYAB5), BREVIPEDICELLUS (CsBP), and AUXIN/INDOLEACETIC ACIDS4 (CsAUX4) and promotes their expression. Knockdown of CsYAB5 resulted in similar phenotypes as CsIVP-RNA interference (RNAi) plants, including disturbed vascular configuration and abnormal organ morphology. Meanwhile, CsIVP-RNAi plants were more resistant to downy mildew and accumulated more salicylic acid (SA). CsIVP physically interacts with NIM1-INTERACTING1 (CsNIMIN1), a negative regulator in the SA signaling pathway. Thus, CsIVP is a novel vasculature regulator functioning in CsYAB5-mediated organ morphogenesis and SA-mediated downy mildew resistance in cucumber.
- Published
- 2020