1. [Effects of matrix metallopeptidase 9 on the proliferation, apoptosis, type I and III procollagen synthesis of rat dermal fibroblasts]
- Author
-
Diao-Zhu, Lin, Chuan, Yang, Li-Hong, Chen, Meng, Ren, Ping, Zhu, Sheng-Neng, Xue, and Li, Yan
- Subjects
Matrix Metalloproteinase 9 ,Animals ,Apoptosis ,Fibroblasts ,Procollagen ,Cell Line ,Cell Proliferation ,Rats - Abstract
To explore the effects of matrix metallopeptidase 9 (MMP-9) on the proliferation, apoptosis, type I and III procollagen synthesis of rat dermal fibroblasts.Lipopolysaccharide (LPS) at 0.1 and 1.0 µg/ml was used to stimulate fibroblasts to up-regulate the expression of MMP-9 for 18 and 48 h. And then RNA interference was employed to inhibit the high expression of MMP-9. Cell proliferation was tested by CCK-8, cell apoptosis by flow cytometry and type I and III procollagen expressions by quantitative reverse transcriptase PCR (qRT-PCR).The MMP-9 expression of fibroblasts increased after the stimulation of LPS. And the 1.0 µg/ml LPS stimulation of 48 hours was 14.25 times of mRNA expression and 2.31 times of protein expression versus that of the normal group (both P0.05). The RNA interference obviously inhibited the high expression of MMP-9. The mRNA expression was 1/8 and protein expression 1/3 (both P0.05) as compared with the control group. Cell proliferation decreased with the rising expression of MMP-9 to some extent [(1.08 ± 0.08) vs (1.18 ± 0.09), P0.05] and improved after the inhibition of high expression of MMP-9 [(1.78 ± 0.17) vs (1.53 ± 0.15), P0.01]. There was no change of apoptosis accompanied with high expression of MMP-9 (P0.05). Apoptosis decreased after the inhibition of high expression of MMP-9 for 48 hours (3.53% ± 0.22% vs 4.47% ± 0.46%, P0.05). The synthesis of type I procollagen was the same no matter up-regulation or down-regulation of MMP-9 expression (P0.05). As the expression of MMP-9 increased, the synthesis of type III procollagen decreased (P0.01), but not increased by 1.02 folds after the inhibited expression of MMP-9 (P0.05).MMP-9 can affect the biological behaviors of rat dermal fibroblasts.
- Published
- 2012