100 results on '"Silano M"'
Search Results
2. Junk food concept: seconds out
- Author
-
Milani, G P, Silano, M, Pietrobelli, A, and Agostoni, C
- Published
- 2017
- Full Text
- View/download PDF
3. Policy implementation and priorities to create healthy food environments using the Healthy Food Environment Policy Index (Food-EPI): A pooled level analysis across eleven European countries
- Author
-
Pineda, E, Poelman, Mp, Aaspõllu, A, Bica, M, Bouzas, C, Carrano, E, De Miguel-Etayo, P, Djojosoeparto, S, Blenkuš, Mg, Graca, P, Geffert, K, Hebestreit, A, Helldan, A, Henjum, S, Huseby, C, Gregório, Mj, Kamphuis, C, Laatikainen, T, Løvhaug, Al, Leydon, C, Luszczynska, A, Mäki, P, Martínez, Ja, Raulio, S, Romaniuk, P, Roos, G, Salvador, C, Sassi, F, Silano, M, Sotlar, I, Specchia, Maria Lucia, de Arriaga, Mt, Terragni, L, Torheim, Le, Tur, Ja, von Philipsborn, P, Harrington, Jm, Vandevijvere, S, Specchia ML (ORCID:0000-0002-3859-4591), Pineda, E, Poelman, Mp, Aaspõllu, A, Bica, M, Bouzas, C, Carrano, E, De Miguel-Etayo, P, Djojosoeparto, S, Blenkuš, Mg, Graca, P, Geffert, K, Hebestreit, A, Helldan, A, Henjum, S, Huseby, C, Gregório, Mj, Kamphuis, C, Laatikainen, T, Løvhaug, Al, Leydon, C, Luszczynska, A, Mäki, P, Martínez, Ja, Raulio, S, Romaniuk, P, Roos, G, Salvador, C, Sassi, F, Silano, M, Sotlar, I, Specchia, Maria Lucia, de Arriaga, Mt, Terragni, L, Torheim, Le, Tur, Ja, von Philipsborn, P, Harrington, Jm, Vandevijvere, S, and Specchia ML (ORCID:0000-0002-3859-4591)
- Abstract
Background: Food environments have been recognised as highly influential on population diets. Government policies have great potential to create healthy food environments to promote healthy diets. This study aimed to evaluate food environment policy implementation in European countries and identify priority actions for governments to create healthy food environments. Methods: The Healthy Food Environment Policy Index (Food-EPI) was used to evaluate the level of food environment policy and infrastructure support implementation in Estonia, Finland, Germany, Ireland, Italy, the Netherlands, Norway, Poland, Portugal, Slovenia, and Spain in 2019-2021. Evidence of implementation of food environment policies was compiled in each country and validated by government officials. National experts evaluated the implementation of policies and identified priority recommendations. Findings: Finland had the highest proportion (32%, n = 7/22) of policies shaping food environments with a "high" level of implementation. Slovenia and Poland had the highest proportion of policies rated at very low implementation (42%, n = 10/24 and 36%, n = 9/25 respectively). Policies regarding food provision, promotion, retail, funding, monitoring, and health in all policies were identified as the most important gaps across the European countries. Experts recommended immediate action on setting standards for nutrients of concern in processed foods, improvement of school food environments, fruit and vegetable subsidies, unhealthy food and beverage taxation, and restrictions on unhealthy food marketing to children. Interpretation: Immediate implementation of policies and infrastructure support that prioritize action towards healthy food environments is urgently required to tackle the burden of obesity and diet-related non-communicable diseases in Europe
- Published
- 2022
4. Implementation level of best practice policies by Italian Government for healthier food environments: Healthy Food Environment Policy Index (Food-EPI)
- Author
-
Zace, D, Di Pilla, A, Silano, M, Carini, E, Cacciatore, P, Frisicale, EM, Vandevijvere, S, Sassi, F, and Specchia, ML
- Abstract
Background. This work aims to identify policies implemented for healthy food environments in Italy within The Healthy Food Environment Policy Index (Food-EPI) project. Methods. Food-EPI tool, which includes two components, thirteen domains and fifty good practice indicators, was adapted for the Italian context. Evidence for implementation was gathered and summarized for all fifty indicators from data sources such as governmental websites, non-government organizations publications and websites and via direct contact with Government officials. Results. The highest level of evidence was found within five domains: food composition (2/2 indicators), labelling (3/4), promotion (4/5), provision (4/5) and leadership (5/5). The domains with less identified evidence were food prices (1/4), food in retail (0/4), trade and investment (0/2) and platforms and interaction (1/4). Conclusions. The evidence summarization and the upcoming stakeholders’ meeting to rate the level of implementation for each indicator in Italy have the potential to improve Government commitment to shape healthier food environments.
- Published
- 2022
5. Autophagy suppresses the pathogenic immune response to dietary antigens in cystic fibrosis
- Author
-
Villella, V. R., Esposito, S., Ferrari, E., Monzani, R., Tosco, A., Rossin, F., Castaldo, A., Silano, M., Marseglia, G. L., Romani, L., Barlev, N. A., Piacentini, M., Raia, V., Kroemer, G., Maiuri, L., IRCCS San Raffaele Scientific Institute [Milan, Italie], Università degli studi di Napoli Federico II, Università degli Studi di Roma Tor Vergata [Roma], Istituto Superiore di Sanita [Rome], Università degli Studi di Perugia (UNIPG), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut Gustave Roussy (IGR), Service de biologie [CHU HEGP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Karolinska Institutet [Stockholm], Karolinska University Hospital [Stockholm], Villella, Vr, Esposito, S, Ferrari, E, Monzani, R, Tosco, A, Rossin, F, Castaldo, A, Silano, M, Marseglia, Gl, Romani, L, Barlev, Na, Piacentini, M, Raia, V, Kroemer, G, Maiuri, L, IRCSS San Raffaele Scientific Institute [Milan, Italie], École pratique des hautes études (EPHE)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), and Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)
- Subjects
Heterozygote ,Settore BIO/06 ,Cystic Fibrosis ,AUTOIMMUNITY ,Cysteamine ,Cystic Fibrosis Transmembrane Conductance Regulator ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Article ,Gliadin ,EPITHELIAL STRESS ,ACTIVATION ,Mice ,INFLAMMATION ,GTP-Binding Proteins ,Autophagy ,Animals ,Humans ,TISSUE TRANSGLUTAMINASE ,Protein Glutamine gamma Glutamyltransferase 2 ,lcsh:QH573-671 ,Mice, Knockout ,Transglutaminases ,lcsh:Cytology ,Homozygote ,CELIAC-DISEASE ,Epithelial Cells ,TRIGGERS ,digestive system diseases ,Peptide Fragments ,Mice, Inbred C57BL ,T-CELLS ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,Cytokines ,GLIADIN ,CYSTEAMINE ,Beclin-1 ,Caco-2 Cells - Abstract
International audience; Under physiological conditions, a finely tuned system of cellular adaptation allows the intestinal mucosa to maintain the gut barrier function while avoiding excessive immune responses to non-self-antigens from dietary origin or from commensal microbes. This homeostatic function is compromised in cystic fibrosis (CF) due to loss-of-function mutations in the CF transmembrane conductance regulator (CFTR). Recently, we reported that mice bearing defective CFTR are abnormally susceptible to a celiac disease-like enteropathy, in thus far that oral challenge with the gluten derivative gliadin elicits an inflammatory response. However, the mechanisms through which CFTR malfunction drives such an exaggerated response to dietary protein remains elusive. Here we demonstrate that the proteostasis regulator/transglutaminase 2 (TGM2) inhibitor cysteamine restores reduced Beclin 1 (BECN1) protein levels in mice bearing cysteamine-rescuable F508del-CFTR mutant, either in homozygosis or in compound heterozygosis with a null allele, but not in knock-out CFTR mice. When cysteamine restored BECN1 expression, autophagy was increased and gliadin-induced inflammation was reduced. The beneficial effects of cysteamine on F508del-CFTR mice were lost when these mice were backcrossed into a Becn1 haploinsufficient/autophagy-deficient background. Conversely, the transfection-enforced expression of BECN1 in human intestinal epithelial Caco-2 cells mitigated the pro-inflammatory cellular stress response elicited by the gliadin-derived P31-43 peptide. In conclusion, our data provide the proof-of-concept that autophagy stimulation may mitigate the intestinal malfunction of CF patients.
- Published
- 2019
6. Correction: PD-L1 in small bowel adenocarcinoma is associated with etiology and tumor-infiltrating lymphocytes, in addition to microsatellite instability (Modern Pathology, (2020), 33, 7, (1398-1409), 10.1038/s41379-020-0497-0)
- Author
-
Giuffrida, P., Arpa, G., Grillo, F., Klersy, C., Sampietro, G., Ardizzone, S., Fociani, P., Fiocca, R., Latella, G., Sessa, F., D'Errico, A., Malvi, D., Mescoli, C., Rugge, M., Nesi, G., Ferrero, S., Furlan, D., Poggioli, G., Rizzello, F., Macciomei, M. C., Santini, D., Volta, U., De Giorgio, R., Caio, G., Calabro, A., Ciacci, C., D'Armiento, M., Rizzo, A., Solina, G., Martino, M., Tonelli, F., Villanacci, V., Cannizzaro, R., Canzonieri, V., Florena, A. M., Biancone, L., Monteleone, G., Caronna, R., Ciardi, A., Elli, L., Caprioli, F., Vecchi, M., D'Inca, R., Zingone, F., D'Odorico, A., Lenti, M. V., Oreggia, B., Bonetti, L. R., Astegiano, M., Biletta, E., Cantoro, L., Giannone, A. G., Orlandi, A., Papi, C., Perfetti, V., Quaquarini, E., Sandri, G., Silano, M., Usai, P., Barresi, V., Ciccocioppo, R., Luinetti, O., Pedrazzoli, P., Pietrabissa, A., Viglio, A., Paulli, M., Corazza, G. R., Solcia, E., Vanoli, A., and Di Sabatino, A.
- Published
- 2020
7. Role of extracellular microvesicles in celiac disease as potential pathogenetic agents and biomarkers of intestinal inflammation
- Author
-
D'Ambrosio, A, Margutti, P, Vincentini, O, Donato, G, Zamboni, S, Sassano, S, Silano, M, Greco, N, Di Loreto, F, Borghini, R, and Picarelli, A
- Subjects
microvescicles ,inflammation ,biomarkers ,celiac disease - Published
- 2020
8. Implementation level of best practice policies by Italian government for healthier food environments
- Author
-
Zace, D, primary, Specchia, M L, additional, Di Pilla, A, additional, Carini, E, additional, Cacciatore, P, additional, Frisicale, E, additional, Silano, M, additional, Ricciardi, W, additional, and Sassi, F, additional
- Published
- 2020
- Full Text
- View/download PDF
9. Nuclear fluorescence serum reactivity on monkey oesophagus: a new antibody for the follow-up of coeliac disease?
- Author
-
Picarelli, A, Sabbatella, L, Di Tola, M, Silano, M, Nicolussi, A, DʼInzeo, S, and Coppa, A
- Published
- 2010
- Full Text
- View/download PDF
10. Growth patterns of breast fed and formula fed infants in the first 12 months of life: an Italian study
- Author
-
Agostoni, C, Grandi, F, Giannì, M L, Silano, M, Torcoletti, M, Giovannini, M, and Riva, E
- Published
- 1999
11. Small bowel carcinomas in celiac or Crohn's disease: Distinctive histophenotypic, molecular and histogenetic patterns
- Author
-
Vanoli, A., Di Sabatino, A., Martino, M., Klersy, C., Grillo, F., Mescoli, C., Nesi, G., Volta, U., Fornino, D., Luinetti, O., Fociani, P., Villanacci, V., D'Armiento, F. P., Cannizzaro, R., Latella, G., Ciacci, C., Biancone, L., Paulli, M., Sessa, F., Rugge, M., Fiocca, R., Corazza, G. R., Solcia, E., Ardizzone, S., Astegiano, M., Caio, G., Calabro, A., Canzonieri, V., Cerutti, R., Ciardi, A., Coppola, L., D'Inca, R., Elli, L., Ferrero, S., Florena, A. M., Furlan, D., Giannone, A. G., Giuffrida, P., Macciomei, M. C., Maccioni, A., Monteleone, G., Migliora, P., Orlandi, A., Papi, C., Perfetti, V., Bonetti, L. R., Rizzo, A., Salemme, M., Sandri, G., Sampietro, G., Santeusanio, G., Santini, D., Silano, M., Solina, G., Tonelli, F., Trapani, D., Usai, P., Vanoli, Alessandro, Di Sabatino, Antonio, Martino, Michele, Klersy, Catherine, Grillo, Federica, Mescoli, Claudia, Nesi, Gabriella, Volta, Umberto, Fornino, Daniele, Luinetti, Ombretta, Fociani, Paolo, Villanacci, Vincenzo, D'Armiento, Francesco P., Cannizzaro, Renato, Latella, Giovanni, Ciacci, Carolina, Biancone, Livia, Paulli, Marco, Sessa, Fausto, Rugge, Massimo, Fiocca, Roberto, Corazza, Gino R., Solcia, Enrico, D'Armiento, Francesco P, and Corazza, Gino R
- Subjects
0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,Pathology ,small bowel carcinoma, celiac disease, immunology ,gastrointestinal cancer ,Kaplan-Meier Estimate ,Histogenesis ,Biology ,Small ,Gastroenterology ,Pathology and Forensic Medicine ,NO ,Surgical pathology ,immunology ,inflammatory bowel disease ,03 medical and health sciences ,small bowel carcinoma ,0302 clinical medicine ,Crohn Disease ,Internal medicine ,Intestine, Small ,Intestinal Neoplasms ,medicine ,Carcinoma ,Humans ,2734 small bowel carcinoma, coeliac disease, Crohn's disease ,Proportional Hazards Models ,Retrospective Studies ,Crohn's disease ,Settore MED/12 - Gastroenterologia ,Celiac Disease ,Female ,Middle Aged ,Microsatellite instability ,Anatomical pathology ,medicine.disease ,digestive system diseases ,Intestine ,030104 developmental biology ,Dysplasia ,030220 oncology & carcinogenesis ,Hematopathology ,celiac disease - Abstract
Non-familial small bowel carcinomas are relatively rare and have a poor prognosis. Two small bowel carcinoma subsets may arise in distinct immune-inflammatory diseases (celiac disease and Crohn's disease) and have been recently suggested to differ in prognosis, celiac disease-associated carcinoma cases showing a better outcome, possibly due to their higher DNA microsatellite instability and tumor-infiltrating T lymphocytes. In this study, we investigated the histological structure (glandular vs diffuse/poorly cohesive, mixed or solid), cell phenotype (intestinal vs gastric/pancreatobiliary duct type) and Wnt signaling activation (beta-catenin and/or SOX-9 nuclear expression) in a series of 26 celiac disease-associated small bowel carcinoma, 25 Crohn's disease-associated small bowel carcinoma and 25 sporadic small bowel carcinoma cases, searching for new prognostic parameters. In addition, non-tumor mucosa of celiac and Crohn's disease patients was investigated for epithelial precursor changes (hyperplastic, metaplastic or dysplastic) to help clarify carcinoma histogenesis. When compared with non-glandular structure and non-intestinal phenotype, both glandular structure and intestinal phenotype were associated with a more favorable outcome at univariable or stage-and microsatellite instability/tumor-infiltrating lymphocyte-inclusive multivariable analysis. The prognostic power of histological structure was independent of the clinical groups while the non-intestinal phenotype, associated with poor outcome, was dominant among Crohn's disease-associated carcinoma. Both nuclear beta-catenin and SOX-9 were preferably expressed among celiac disease-associated carcinomas; however, they were devoid, per se, of prognostic value. We obtained findings supporting an origin of celiac disease-associated carcinoma in SOX-9-positive immature hyperplastic crypts, partly through flat beta-catenin-positive dysplasia, and of Crohn's disease-associated carcinoma in a metaplastic (gastric and/or pancreatobiliary-type) mucosa, often through dysplastic polypoid growths of metaplastic phenotype. In conclusion, despite their common origin in a chronically inflamed mucosa, celiac disease-associated and Crohn's disease-associated small bowel carcinomas differ substantially in histological structure, phenotype, microsatellite instability/tumor-infiltrating lymphocyte status, Wnt pathway activation, mucosal precursor lesions and prognosis.
- Published
- 2017
12. Clinical features of chronic C virus hepatitis in patients with celiac disease
- Author
-
Silano, M., Volta, U., Vincentini, O., De Vincenzi, M., Gasbarrini Italian Registry Of The Complications Of Celiac Disease, (., De Vitis, V., Greco, L., Auricchio, S., Santini, D., Scaggiante, F., Vincenzi, M., Federici, Castellano, E., Calvi, A., Sategna, Guidetti, Grosso, S., Campanella, J., Corazza, G. R., Sandri, G., Giorgetti, G., Amici, M., Parisi, C., Lo Perfido, S., Perri, F., Festa, V., Pelli, M. A., Cavalletti, M. L., Segato, S., Curzio, M., Pennazio, M., Rossini, F. P., Picarelli, Antonio, Pera, A., Ercole, E., Passaleva, M. T., Barbato, M., Usai, P., Dore, M. F., Chilovi, F., Piazzi, L., Zancanella, L., Boarino, V., and Ferrari, A.
- Subjects
Microbiology (medical) ,Adult ,Male ,medicine.medical_specialty ,Hepatitis C virus ,Population ,Autoimmune hepatitis ,medicine.disease_cause ,Gastroenterology ,Antiviral Agents ,Coeliac disease ,Primary sclerosing cholangitis ,Primary biliary cirrhosis ,Internal medicine ,medicine ,Prevalence ,Humans ,education ,Hepatitis ,education.field_of_study ,Anemia, Iron-Deficiency ,business.industry ,Depression ,General Medicine ,Hepatitis C ,Hepatitis C, Chronic ,medicine.disease ,Arthralgia ,digestive system diseases ,Celiac Disease ,Infectious Diseases ,Diabetes Mellitus, Type 2 ,Immunology ,Female ,business - Abstract
The association between celiac disease (CD) and several liver disorders has long been documented. About 40% of adult celiac patients have been reported to have mild to moderate hypertransaminasemia (up to five times the upper limit of normal) at the time of diagnosis of CD [1, 2]. In addition, CD has been found in roughly 10% of patients with unexplained hypertransaminasemia, and the majority of them have had their liver enzyme levels normalized after one year of following a strict gluten-free diet [3, 4]. In addition, an increased prevalence of primary biliary cirrhosis, primary sclerosing cholangitis, and autoimmune hepatitis among CD patients has been reported [5, 6]. CD might also be linked to very severe liver conditions such as end-stage liver failure and hepatocellular carcinoma [7]. There is also evidence, even contrasting reports, about the association of CD with nonalcoholic steatohepatitis and fatty liver disease [8]. In contrast, no definitive evidence is available about the association between chronic hepatitis C (hepatitis C virus [HCV]) and CD. Fine et al. described a three-fold increase of CD prevalence among HCV patients compared to noninfected celiac individuals [9]. It has also been reported the activation of silent CD during the antiviral treatment for HCV with interferon-α and ribavirin, both alone and in combination [10]. Consequently, a routine serological screening for CD has been proposed in HCV patients before starting antiviral therapy. In case of HCV positivity, the achievement of the histological normalization of the intestinal mucosa after following a gluten-free diet has been advised before starting the therapy [10]. On the contrary, some recent prospective studies have not shown increased prevalence of CD among HCV patients and reported that the link between these two conditions is biased by the route of transmission [11, 12]. Among the 3,725 celiac patients included in the Italian Registry of the Complication of Celiac Disease, we identified 34 individuals (0.91%) that had an HCV chronic hepatitis at the time of diagnosis of CD. For the diagnosis of HCV, we considered the serological positivity of antiHCV antibodies. Some of the patients had the diagnosis made in the early 1980s, when the molecular tests for the detection of the viral antigens were not yet available. The demographic and clinical features of the patients with both CD and HCV with respect to those of patients with CD only are listed in Table 1. The prevalence of HCV among our celiac series is lower than the overall prevalence of HCV among the general population in Italy, matched for age and gender, which is estimated to be around 2% [13]. This finding does not support the hypothesis of a potential correlation between these two disorders. It has been assumed that antiviral therapy with INF-α and ribavirin may precipitate the onset of CD in susceptible individuals, promoting a Th1-specific response in the small intestine [14]. However, in our series, only 12 of the 34 celiac patients with HCV had antiviral therapy before CD diagnosis. Looking at our series, it seems more likely that an overall increased risk of CD in HCV patients exists, due to the predisposition for autoimmune diseases related to Eur J Clin Microbiol Infect Dis (2009) 28:1267–1269 DOI 10.1007/s10096-009-0769-6
- Published
- 2009
13. The Modern treatment of early gastric cancer: our experience an an Italian cohort
- Author
-
Catalano, F, Trecca, A, Rodella, L, Lombardo, F, Tomezzoli, Anna, Battista, S, Silano, M, Gaj, F, and de Manzoni, G.
- Subjects
Early Gastric Cancer ,Endoscopy ,Endoscopic Resection - Published
- 2009
14. Degradazione del glutine mediante l’utilizzo di batteri lattici e proteasi fungine: nuove prospettive per la celiachia
- Author
-
DE ANGELIS, M., Rizzello, C, DI CAGNO, R., Camarca, A., Silano, M., Losito, I., DE VINCENZI, M., DE BARI, M. D., Palmisano, F., Maurano, F., and Gobbetti, C. GIANFRANI AND M.
- Published
- 2008
15. Degradazione del glutine mediante l’utilizzo di batteri lattici e proteasi fungine: nuove prospettive per la celiachia. II Congresso nazionale
- Author
-
DE ANGELIS, M, Rizzello, C. G., DI CAGNO, R., Camarca, A., Silano, M., Losito, I., DE VINCENZI, M., DE BARI, M. D., Palmisano, F., Maurano, F., and Gobbetti, C. GIANFRANI M.
- Published
- 2008
16. Degradazione del glutine mediante l’utilizzo di batteri lattici e proteasi fungine: nuove prospettive per la celiachia
- Author
-
DE ANGELIS, M, Rizzello, C, DI CAGNO, R, Camarca, A, Silano, M, Losito, I, DE VINCENZI, M, DE BARI, M. D., Palmisano, F, Maurano, F, Gianfrani, C, and Gobbetti, M
- Published
- 2008
17. VSL#3 probiotic preparation has the capacity to hydrolyze gliadin polypeptides responsible for Celiac Sprue
- Author
-
DE ANGELIS, Maria, Rizzello, CARLO GIUSEPPE, Fasano, A, Clemente, M. G., DE SIMONE, C, Silano, M, DE VINCENZI, M, Losito, Ilario, and Gobbetti, Marco
- Subjects
Wheat flour ,CD3+ ,Proteolysis ,Celiac Sprue ,Probiotic ,Gliadin ,Zonulin - Published
- 2006
18. Small bowel malignancy at diagnosis of coeliac disease
- Author
-
Silano, M and De Vincenzi, M
- Subjects
Letter - Published
- 2005
19. Potential new mechanisms of placental damage in celiac disease: anti-transglutaminase antibodies impair human endometrial angiogenesis
- Author
-
Di Simone, Nicoletta, De Spirito, Marco, Di Nicuolo, Fiorella, Tersigni, Chiara, Castellani, Roberta, Silano, M, Maulucci, Giuseppe, Papi, Massimiliano, Marana, R, Scambia, Giovanni, Gasbarrini, Antonio, Di Simone, Nicoletta (ORCID:0000-0003-1273-3335), De Spirito, Marco (ORCID:0000-0003-4260-5107), Maulucci, Giuseppe (ORCID:0000-0002-2154-319X), Papi, Massimiliano (ORCID:0000-0002-0029-1309), Scambia, Giovanni (ORCID:0000-0003-2758-1063), Gasbarrini, Antonio (ORCID:0000-0002-7278-4823), Di Simone, Nicoletta, De Spirito, Marco, Di Nicuolo, Fiorella, Tersigni, Chiara, Castellani, Roberta, Silano, M, Maulucci, Giuseppe, Papi, Massimiliano, Marana, R, Scambia, Giovanni, Gasbarrini, Antonio, Di Simone, Nicoletta (ORCID:0000-0003-1273-3335), De Spirito, Marco (ORCID:0000-0003-4260-5107), Maulucci, Giuseppe (ORCID:0000-0002-2154-319X), Papi, Massimiliano (ORCID:0000-0002-0029-1309), Scambia, Giovanni (ORCID:0000-0003-2758-1063), and Gasbarrini, Antonio (ORCID:0000-0002-7278-4823)
- Abstract
Celiac disease (CD) is an autoimmune enteropathy triggered by gluten ingestion and characterized by circulating anti-transglutaminase type 2 (anti-TG2) autoantibodies. An epidemiological link between maternal CD and increased risk of pregnancy failure has been established; however, the mechanism underlying this association is still poorly understood. Because proper endometrial angiogenesis and decidualization are prerequisites for placental development, we investigated the effect of anti-TG2 antibodies on the process of endometrial angiogenesis. Binding of anti-TG2 antibodies to human endometrial endothelial cells (HEECs) was evaluated by ELISA. Angiogenesis was studied in vitro on HEECs and in vivo in a murine model. In particular, we investigated the effect of anti-TG2 antibodies on HEEC matrix metalloprotease-2 (MMP-2) activity by gelatin zymography, cytoskeletal organization and membrane properties by confocal microscopy, and activation of extracellular signal-regulated kinases (ERKs) and focal adhesion kinase (FAK) by Western blot analysis. Anti-TG2 antibodies bound to HEECs and decreased newly formed vessels both in vitro and in vivo. Anti-TG2 antibodies impaired angiogenesis by inhibiting the activation of MMP-2, disarranging cytoskeleton fibers, changing the physical and mechanical properties of cell membranes, and inhibiting the intracellular phosphorylation of FAK and ERK. Anti-TG2 antibodies inhibit endometrial angiogenesis affecting the TG2-dependent migration of HEECs and extracellular matrix degradation, which are necessary to form new vessels. Our results identify pathogenic mechanisms of placental damage in CD.
- Published
- 2013
20. Potential new mechanisms of placental damage in celiac disease: anti-transglutaminase antibodies impair human endometrial angiogenesis
- Author
-
De Spirito, Marco, Di Nicuolo, Fiorella, Tersigni, Chiara, Castellani, Roberta, Silano, M, Maulucci, Giuseppe, Papi, Massimiliano, Marana, Riccardo, Scambia, Giovanni, Gasbarrini, Antonio, De Spirito, Marco (ORCID:0000-0003-4260-5107), Maulucci, Giuseppe (ORCID:0000-0002-2154-319X), Papi, Massimiliano (ORCID:0000-0002-0029-1309), Marana, Riccardo (ORCID:0000-0003-1616-7836), Scambia, Giovanni (ORCID:0000-0003-2758-1063), Gasbarrini, Antonio (ORCID:0000-0002-7278-4823), De Spirito, Marco, Di Nicuolo, Fiorella, Tersigni, Chiara, Castellani, Roberta, Silano, M, Maulucci, Giuseppe, Papi, Massimiliano, Marana, Riccardo, Scambia, Giovanni, Gasbarrini, Antonio, De Spirito, Marco (ORCID:0000-0003-4260-5107), Maulucci, Giuseppe (ORCID:0000-0002-2154-319X), Papi, Massimiliano (ORCID:0000-0002-0029-1309), Marana, Riccardo (ORCID:0000-0003-1616-7836), Scambia, Giovanni (ORCID:0000-0003-2758-1063), and Gasbarrini, Antonio (ORCID:0000-0002-7278-4823)
- Abstract
Celiac disease (CD) is an autoimmune enteropathy triggered by gluten ingestion and characterized by circulating anti-transglutaminase type 2 (anti-TG2) autoantibodies. An epidemiological link between maternal CD and increased risk of pregnancy failure has been established; however, the mechanism underlying this association is still poorly understood. Because proper endometrial angiogenesis and decidualization are prerequisites for placental development, we investigated the effect of anti-TG2 antibodies on the process of endometrial angiogenesis. Binding of anti-TG2 antibodies to human endometrial endothelial cells (HEECs) was evaluated by ELISA. Angiogenesis was studied in vitro on HEECs and in vivo in a murine model. In particular, we investigated the effect of anti-TG2 antibodies on HEEC matrix metalloprotease-2 (MMP-2) activity by gelatin zymography, cytoskeletal organization and membrane properties by confocal microscopy, and activation of extracellular signal-regulated kinases (ERKs) and focal adhesion kinase (FAK) by Western blot analysis. Anti-TG2 antibodies bound to HEECs and decreased newly formed vessels both in vitro and in vivo. Anti-TG2 antibodies impaired angiogenesis by inhibiting the activation of MMP-2, disarranging cytoskeleton fibers, changing the physical and mechanical properties of cell membranes, and inhibiting the intracellular phosphorylation of FAK and ERK. Anti-TG2 antibodies inhibit endometrial angiogenesis affecting the TG2-dependent migration of HEECs and extracellular matrix degradation, which are necessary to form new vessels. Our results identify pathogenic mechanisms of placental damage in CD.
- Published
- 2013
21. 31-43 AMINOACIDIC SEQUENCE OF THE ALPHA-GLIADIN INDUCES ANTIENDOMYSIAL ANTIBODY PRODUCTION DURING IN VITRO CHALLENGE
- Author
-
Picarelli, Antonio, DI TOLA, M, Sabbatella, L, Anania, Mc, DI CELLO, T, Greco, R, Silano, M, and DE VINCENZI, M.
- Published
- 1999
22. Anti-tissue transglutaminase antibodies from celiac patients are responsible for trophoblast damage via apoptosis in vitro
- Author
-
Di Simone, Nicoletta, Silano, M, Castellani, Roberta, Di Nicuolo, Fiorella, D'Alessio, Maria Clara, Franceschi, Francesco, Tritarelli, Alessandra, Leone, Antonio Maria, Tersigni, Chiara, Gasbarrini, Giovanni Battista, Gentiloni Silveri, Nicolo', Caruso, Alessandro, Gasbarrini, Antonio, Di Simone, Nicoletta (ORCID:0000-0003-1273-3335), Franceschi, Francesco (ORCID:0000-0001-6266-445X), Leone, Antonio Maria (ORCID:0000-0002-1276-9883), Caruso, Alessandro (ORCID:0000-0002-4749-3207), Gasbarrini, Antonio (ORCID:0000-0002-7278-4823), Di Simone, Nicoletta, Silano, M, Castellani, Roberta, Di Nicuolo, Fiorella, D'Alessio, Maria Clara, Franceschi, Francesco, Tritarelli, Alessandra, Leone, Antonio Maria, Tersigni, Chiara, Gasbarrini, Giovanni Battista, Gentiloni Silveri, Nicolo', Caruso, Alessandro, Gasbarrini, Antonio, Di Simone, Nicoletta (ORCID:0000-0003-1273-3335), Franceschi, Francesco (ORCID:0000-0001-6266-445X), Leone, Antonio Maria (ORCID:0000-0002-1276-9883), Caruso, Alessandro (ORCID:0000-0002-4749-3207), and Gasbarrini, Antonio (ORCID:0000-0002-7278-4823)
- Abstract
OBJECTIVES: The association between maternal celiac disease (CD) and both reduced fertility and increased risk of adverse pregnancy-related events has been long documented. However, no evidences are available regarding the pathogenic mechanisms of this link. The aim of this study was to determine whether anti-tissue transglutaminase (anti-tTG) antibodies are involved in the damage of trophoblastic cells in vitro . METHODS: Human primary trophoblastic cells, isolated from term placenta, were exposed to anti-tTG immunoglobulin G (IgG) antibodies, both commercially available and separated from sera of three untreated celiac women. The ability of anti-tTG antibodies to bind to trophoblastic cells, invasiveness of placental cells through a layer of extracellular matrix, and the activity of cellular matrix metalloprotease (MMP) and cellular apoptosis were evaluated, as indicators of trophoblast damage, by TdT-mediated dUTP digoxigenin nick end labeling (TUNEL) and annexin V expression. RESULTS: Anti-tTG IgG showed a specifi c dose- and time-dependent binding to human trophoblast. In addition, trophoblastic cells, after being exposed to anti-tTG IgG antibodies, both commercially available and separated from sera of celiac women, showed an impaired invasiveness, a decreased activity of cellular MMP, and a greater percentage of TUNEL positivity and annexin V positivity. CONCLUSIONS: We showed that the binding of anti-tTG antibodies to trophoblast might represent a key mechanism by which the embryo implantation and pregnancy outcome are impaired in untreated celiac pregnant women. Because healthy trophoblast development is essential for placental and fetal development, these data provide a novel mechanism for CD-induced infertility, early pregnancy loss, and intrauterine growth retardation.
- Published
- 2010
23. GROWTH INDEXES AT BIRTH AND 1 YEAR OF AGE IN ITALIAN INFANTS BREASTFED FOR 12 MONTHS
- Author
-
Agostoni, C, primary, Grandi, F, additional, Torcoletti, M, additional, Silano, M, additional, Gianni, M. L, additional, Giovannini, M, additional, and Riva, E, additional
- Published
- 1999
- Full Text
- View/download PDF
24. Association Between the Use of the Pacifier and Shorter Duration of Breastfeeding in Italy
- Author
-
Banderali, G, primary, Radaelli, G, additional, Agostoni, C, additional, Silano, M, additional, Giovannini, M, additional, and Riva, E, additional
- Published
- 1998
- Full Text
- View/download PDF
25. Geographical Differences of Breastfeeding Rates in Italy
- Author
-
Banderali, G, primary, Radaelli, G, additional, Agostoni, C, additional, Silano, M, additional, Giovannini, M, additional, and Riva, E, additional
- Published
- 1998
- Full Text
- View/download PDF
26. Delayed diagnosis of coeliac disease increases cancer risk
- Author
-
Di Benedetto Rita, Dessì Mariarita, Mecchia Anna, Volta Umberto, Silano Marco, and De Vincenzi Massimo
- Subjects
Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Abstract Background The association between coeliac disease (CD) and neoplasms has been long established, but few data are available about the risk factors. The aim of this paper is to estimate the risk of developing a neoplasm among non diagnosed coeliac patients and to evaluate if this risk correlates with the age of patients at diagnosis of coeliac disease. Methods The study population consists of patients (n = 1968) diagnosed with CD at 20 Italian gastroenterology referral Centers between 1st January 1982 and 31st March 2005. Results The SIR for all cancers resulted to be 1.3; 95% CI = 1.0–1.7 p < 0.001. The specific SIRs for non Hodgkin lymphoma was 4.7; 95% CI = 2.9–7.3 p < 0.001, for the small bowel carcinoma 25; 95% CI = 8.5–51.4 p < 0.001, for non Hodgkin lymphoma 10; 95% CI = 2.7–25 p = 0.01, finally for the stomach carcinoma 3; 95% CI = 1.3–4.9 p < 0.08. The mean age at diagnosis of CD of patients that developed sooner or later a neoplasm was 47,6 ± 10.2 years versus 28.6 ± 18.2 years of patients who did not. Conclusion Coeliac patients have an increased risk of developing cancer in relation to the age of diagnosis of CD. This risk results higher for malignancies of the gastro-intestinal sites. An accurate screening for tumors should be performed in patients diagnosed with CD in adulthood and in advancing age.
- Published
- 2007
- Full Text
- View/download PDF
27. PD-L1 in small bowel adenocarcinoma is associated with etiology and tumor-infiltrating lymphocytes, in addition to microsatellite instability
- Author
-
Giovanni Arpa, Gabriella Nesi, Catherine Klersy, Carolina Ciacci, Antonietta D'Errico, Anna D'Odorico, Marco Paulli, Gino Roberto Corazza, Fausto Sessa, Valeria Barresi, Vittorio Perfetti, Federica Grillo, Vincenzo Canzonieri, Renato Cannizzaro, Roberto Fiocca, Stefano Ferrero, Luca Reggiani Bonetti, Deborah Malvi, Giovanni Latella, Paolo Pedrazzoli, Antonio Calabrò, Roberto De Giorgio, Alessandra Viglio, Fernando Rizzello, Flavio Caprioli, Roberto Caronna, Daniela Furlan, Antonino Giulio Giannone, Marco Silano, Maurizio Vecchi, Michele Martino, Francesco Tonelli, Laura Cantoro, Antonio Di Sabatino, Maria D'Armiento, Enrico Solcia, Paolo Giuffrida, Gianluca M. Sampietro, Ada Maria Florena, Giovanni Monteleone, Livia Biancone, Claudia Mescoli, G. Solina, Andrea Pietrabissa, Umberto Volta, Renata D'Incà, Ombretta Luinetti, Vincenzo Villanacci, Luca Elli, Massimo Rugge, Maria Cristina Macciomei, Paolo Fociani, Marco Astegiano, Rachele Ciccocioppo, Fabiana Zingone, Claudio Papi, Giacomo Caio, G. Sandri, Barbara Oreggia, Alessandro Vanoli, Aroldo Rizzo, Elena Biletta, Augusto Orlandi, Gilberto Poggioli, Antonio Ciardi, Marco Vincenzo Lenti, Paolo Usai, Erica Quaquarini, Donatella Santini, Sandro Ardizzone, Giuffrida, Paolo, Arpa, Giovanni, Grillo, Federica, Klersy, Catherine, Sampietro, Gianluca, Ardizzone, Sandro, Fociani, Paolo, Fiocca, Roberto, Latella, Giovanni, Sessa, Fausto, D'Errico, Antonietta, Malvi, Deborah, Mescoli, Claudia, Rugge, Massimo, Nesi, Gabriella, Ferrero, Stefano, Furlan, Daniela, Poggioli, Gilberto, Rizzello, Fernando, Macciomei, Maria C, Santini, Donatella, Volta, Umberto, De Giorgio, Roberto, Caio, Giacomo, Calabrò, Antonio, Ciacci, Carolina, D'Armiento, Maria, Rizzo, Aroldo, Solina, Gaspare, Martino, Michele, Tonelli, Francesco, Villanacci, Vincenzo, Cannizzaro, Renato, Canzonieri, Vincenzo, Florena, Ada M, Biancone, Livia, Monteleone, Giovanni, Caronna, Roberto, Ciardi, Antonio, Elli, Luca, Caprioli, Flavio, Vecchi, Maurizio, D'Incà, Renata, Zingone, Fabiana, D'Odorico, Anna, Lenti, Marco Vincenzo, Oreggia, Barbara, Reggiani Bonetti, Luca, Astegiano, Marco, Biletta, Elena, Cantoro, Laura, Giannone, Antonino G, Orlandi, Augusto, Papi, Claudio, Perfetti, Vittorio, Quaquarini, Erica, Sandri, Giancarlo, Silano, Marco, Usai, Paolo, Barresi, Valeria, Ciccocioppo, Rachele, Luinetti, Ombretta, Pedrazzoli, Paolo, Pietrabissa, Andrea, Viglio, Alessandra, Paulli, Marco, Corazza, Gino R, Solcia, Enrico, Vanoli, Alessandro, Di Sabatino, Antonio, Giuffrida P., Arpa G., Grillo F., Klersy C., Sampietro G., Ardizzone S., Fociani P., Fiocca R., Latella G., Sessa F., D'Errico A., Malvi D., Mescoli C., Rugge M., Nesi G., Ferrero S., Furlan D., Poggioli G., Rizzello F., Macciomei M.C., Santini D., Volta U., De Giorgio R., Caio G., Calabro A., Ciacci C., D'Armiento M., Rizzo A., Solina G., Martino M., Tonelli F., Villanacci V., Cannizzaro R., Canzonieri V., Florena A.M., Biancone L., Monteleone G., Caronna R., Ciardi A., Elli L., Caprioli F., Vecchi M., D'Inca R., Zingone F., D'Odorico A., Lenti M.V., Oreggia B., Reggiani Bonetti L., Astegiano M., Biletta E., Cantoro L., Giannone A.G., Orlandi A., Papi C., Perfetti V., Quaquarini E., Sandri G., Silano M., Usai P., Barresi V., Ciccocioppo R., Luinetti O., Pedrazzoli P., Pietrabissa A., Viglio A., Paulli M., Corazza G.R., Solcia E., Vanoli A., Di Sabatino A., Giuffrida, P., Arpa, G., Grillo, F., Klersy, C., Sampietro, G., Ardizzone, S., Fociani, P., Fiocca, R., Latella, G., Sessa, F., D'Errico, A., Malvi, D., Mescoli, C., Rugge, M., Nesi, G., Ferrero, S., Furlan, D., Poggioli, G., Rizzello, F., Macciomei, M. C., Santini, D., Volta, U., De Giorgio, R., Caio, G., Calabro, A., Ciacci, C., D'Armiento, M., Rizzo, A., Solina, G., Martino, M., Tonelli, F., Villanacci, V., Cannizzaro, R., Canzonieri, V., Florena, A. M., Biancone, L., Monteleone, G., Caronna, R., Ciardi, A., Elli, L., Caprioli, F., Vecchi, M., D'Inca, R., Zingone, F., D'Odorico, A., Lenti, M. V., Oreggia, B., Reggiani Bonetti, L., Astegiano, M., Biletta, E., Cantoro, L., Giannone, A. G., Orlandi, A., Papi, C., Perfetti, V., Quaquarini, E., Sandri, G., Silano, M., Usai, P., Barresi, V., Ciccocioppo, R., Luinetti, O., Pedrazzoli, P., Pietrabissa, A., Viglio, A., Paulli, M., Corazza, G. R., Solcia, E., Vanoli, A., Di Sabatino, A., and Vincenzo Lenti, Marco
- Subjects
0301 basic medicine ,Male ,PD-L1 - small bowel adenocarcinoma - tumor-infiltrating lymphocytes - microsatellite instability ,Pathology ,BLOCKADE ,Colorectal cancer ,Lymphocyte ,Small bowel adenocarcinoma ,Gastroenterology ,B7-H1 Antigen ,Settore MED/12 ,0302 clinical medicine ,Crohn Disease ,Intestine, Small ,small bowel adenocarcinoma ,Small bowel adenocarcinomas ,MEDULLARY CARCINOMA ,MORPHOLOGY ,EXPRESSION ,CANCER ,biology ,microsatelliteinstability ,Middle Aged ,medicine.anatomical_structure ,Medullary carcinoma ,tumor infiltrating lymphocytes ,030220 oncology & carcinogenesis ,tumor-infiltrating lymphocytes ,Adenocarcinoma ,Female ,Microsatellite Instability ,PD-L1 ,Adult ,medicine.medical_specialty ,small bowel adenocarcinoma, tumor-infiltrating lymphocytes, microsatelliteinstability ,Settore MED/08 - Anatomia Patologica ,PD-L1, small bowel adenocarcinoma ,NO ,Pathology and Forensic Medicine ,03 medical and health sciences ,Lymphocytes, Tumor-Infiltrating ,Internal medicine ,expression ,Intestinal Neoplasms ,Biomarkers, Tumor ,medicine ,Humans ,PD-L1 in small bowel adenocarcinoma, MSI-H ,Small bowel adenocarcinoma, expression, microsatellite instability, biomarkers ,Aged ,Retrospective Studies ,business.industry ,Tumor-infiltrating lymphocytes ,biomarkers ,Cancer ,Correction ,Microsatellite instability ,medicine.disease ,Celiac Disease ,030104 developmental biology ,biology.protein ,Etiology ,business - Abstract
Small bowel adenocarcinomas (SBAs) are often associated with poor prognosis and have limited therapeutic options. Programmed cell death protein-1 (PD-1)/programmed cell death ligand 1 (PD-L1) pathway blockade is an effective treatment in many microsatellite instability-high (MSI-H) solid tumors. We aimed at investigating PD-L1 and PD-1 expression in non-hereditary, non-ampullary SBAs, associated with celiac disease (CeD), Crohn’s disease (CrD), or sporadic, recruited through the Small Bowel Cancer Italian Consortium. We assessed PD-L1 and PD-1 by immunohistochemistry in a series of 121 surgically resected SBAs, including 34 CeD-SBAs, 49 CrD-SBAs, and 38 sporadic SBAs. PD-L1 and PD-1 expression was correlated with several clinico-pathological features, such as the etiology, microsatellite instability status, and tumor-infiltrating lymphocyte (TIL) density. The prevalence of PD-L1 positivity according to combined positive score (CPS) was 26% in the whole cohort of SBAs, with significantly (p = 0.001) higher percentage (35%) in both CeD-SBAs and CrD-SBAs in comparison with sporadic SBAs (5%). CPS ≥ 1 SBAs were significantly (p = 0.013) more frequent in MSI-H cases (41%) than in non-MSI-H ones (18%); however, 15 CPS ≥ 1 microsatellite stable SBAs were also identified. CPS ≥ 1 SBAs showed higher TIL and PD-1+ immune cell density, more frequently medullary histotype, as well as a better outcome in comparison with CPS < 1 cases. This study demonstrates an increased proportion of PD-L1+ cases in both CeD-SBAs and CrD-SBAs in comparison with sporadic SBAs. In addition, the identification of a subset of PD-L1+ microsatellite stable SBAs supports the need to ascertain additional biomarkers of response to immune checkpoint inhibitors along with MSI-H.
- Published
- 2020
28. Small Bowel Carcinomas in Coeliac or Crohn’s Disease: Clinico-pathological, Molecular, and Prognostic Features. A Study From the Small Bowel Cancer Italian Consortium
- Author
-
Claudia Mescoli, Michele Martino, Fausto Sessa, Massimo Rugge, Ombretta Luinetti, Gabriella Nesi, Vincenzo Canzonieri, Alessandro Vanoli, Luca Reggiani Bonetti, Ada Maria Florena, Giovanni Monteleone, Daniela Furlan, Umberto Volta, Paolo Fociani, Vincenzo Villanacci, Antonino Giulio Giannone, Marco Silano, Antonio Di Sabatino, Antonio Maccioni, Paolo Usai, G. Solina, Vittorio Perfetti, Federica Grillo, Maria Cristina Macciomei, Rachele Manca, Stefano Ferrero, Renato Cannizzaro, Aroldo Rizzo, Livia Biancone, Luca Elli, Claudio Papi, Giacomo Caio, Giovanni Latella, Antonio Calabrò, Roberta Cerutti, Marianna Salemme, Paolo Giuffrida, Gianluca M. Sampietro, Gino Roberto Corazza, Paola Migliora, Donatella Santini, Sandro Ardizzone, Augusto Orlandi, Francesco Tonelli, Antonio Ciardi, Catherine Klersy, Carolina Ciacci, Davide Trapani, Renata D'Incà, Francesco Paolo D'Armiento, Marco Paulli, Marco Astegiano, Roberto Caronna, Roberto Fiocca, Luigi Michele Coppola, Paola Alberizzi, Enrico Solcia, Giuseppe Santeusanio, G. Sandri, Roberta Riboni, Vanoli, Alessandro, Sabatino, Antonio Di, Furlan, Daniela, Klersy, Catherine, Grillo, Federica, Fiocca, Roberto, Mescoli, Claudia, Rugge, Massimo, Nesi, Gabriella, Fociani, Paolo, Sampietro, Gianluca, Ardizzone, Sandro, Luinetti, Ombretta, Calabrò, Antonio, Tonelli, Francesco, Volta, Umberto, Santini, Donatella, Caio, Giacomo, Giuffrida, Paolo, Elli, Luca, Ferrero, Stefano, Latella, Giovanni, Ciardi, Antonio, Caronna, Roberto, Solina, Gaspare, Rizzo, Aroldo, Ciacci, Carolina, D'Armiento, FRANCESCO PAOLO, Salemme, Marianna, Villanacci, Vincenzo, Cannizzaro, Renato, Canzonieri, Vincenzo, Bonetti, Luca Reggiani, Biancone, Livia, Monteleone, Giovanni, Orlandi, Augusto, Santeusanio, Giuseppe, Macciomei, Maria C, D'Incà, Renata, Perfetti, Vittorio, Sandri, Giancarlo, Silano, Marco, Florena, Ada M, Giannone, Antonino G, Papi, Claudio, Coppola, Luigi, Usai, Paolo, Maccioni, Antonio, Astegiano, Marco, Migliora, Paola, Manca, Rachele, Martino, Michele, Trapani, Davide, Cerutti, Roberta, Alberizzi, Paola, Riboni, Roberta, Sessa, Fausto, Paulli, Marco, Solcia, Enrico, Corazza, Gino R., Di Sabatino, Antonio, D’Armiento, Francesco P., Reggiani Bonetti, Luca, Macciomei, Maria C., D’Incà, Renata, Florena, Ada M., Giannone, Antonino G., Vanoli A., Di Sabatino A., Furlan D., Klersy C., Grillo F., Fiocca R., Mescoli C., Rugge M., Nesi G., Fociani P., Sampietro G., Ardizzone S., Luinetti O., Calabro A., Tonelli F., Volta U., Santini D., Caio G., Giuffrida P., Elli L., Ferrero S., Latella G., Ciardi A., Caronna R., Solina G., Rizzo A., Ciacci C., D'Armiento F.P., Salemme M., Villanacci V., Cannizzaro R., Canzonieri V., Bonetti L.R., Biancone L., Monteleone G., Orlandi A., Santeusanio G., Macciomei M.C., D'Inca R., Perfetti V., Sandri G., Silano M., Florena A.M., Giannone A.G., Papi C., Coppola L., Usai P., Maccioni A., Astegiano M., Migliora P., Manca R., Martino M., Trapani D., Cerutti R., Alberizzi P., Riboni R., Sessa F., Paulli M., Solcia E., and Corazza G.R.
- Subjects
Male ,0301 basic medicine ,Neuroblastoma RAS viral oncogene homolog ,Oncology ,Survival ,Receptor, ErbB-2 ,Colorectal cancer ,medicine.disease_cause ,Inflammatory bowel disease ,tumour-infiltrating lymphocyte ,ErbB-2 ,0302 clinical medicine ,Crohn Disease ,Retrospective Studie ,Risk Factors ,80 and over ,Child ,Class I Phosphatidylinositol 3-Kinase ,Aged, 80 and over ,Colonic Neoplasm ,Settore MED/12 - Gastroenterologia ,Crohn's disease ,MLH1 methylation ,Tumour-infiltrating lymphocytes ,Gastroenterology ,General Medicine ,Middle Aged ,Prognosis ,Microsatellite instability ,MLH1 promoter methylation ,030220 oncology & carcinogenesis ,Colonic Neoplasms ,Survival Analysi ,KRAS ,Human ,Receptor ,Adult ,Proto-Oncogene Proteins B-raf ,medicine.medical_specialty ,Prognosi ,Class I Phosphatidylinositol 3-Kinases ,Settore MED/08 - Anatomia Patologica ,NO ,Proto-Oncogene Proteins p21(ras) ,Young Adult ,03 medical and health sciences ,Internal medicine ,medicine ,Carcinoma ,Humans ,inflammatory bowel disease ,microsatellite instability ,survival ,tumour-infiltrating lymphocytes ,neoplasms ,Aged ,Retrospective Studies ,Celiac Disease ,Microsatellite Instability ,Survival Analysis ,Tumor Suppressor Protein p53 ,business.industry ,Tumour-infiltrating lymphocyte ,Risk Factor ,Cancer ,medicine.disease ,eye diseases ,digestive system diseases ,030104 developmental biology ,business - Abstract
Background and aims An increased risk of small bowel carcinoma [SBC] has been reported in coeliac disease [CD] and Crohn's disease [CrD]. We explored clinico-pathological, molecular, and prognostic features of CD-associated SBC [CD-SBC] and CrD-associated SBC [CrD-SBC] in comparison with sporadic SBC [spo-SBC]. Methods A total of 76 patients undergoing surgical resection for non-familial SBC [26 CD-SBC, 25 CrD-SBC, 25 spo-SBC] were retrospectively enrolled to investigate patients' survival and histological and molecular features including microsatellite instability [MSI] and KRAS/NRAS, BRAF, PIK3CA, TP53, HER2 gene alterations. Results CD-SBC showed a significantly better sex-, age-, and stage-adjusted overall and cancer-specific survival than CrD-SBC, whereas no significant difference was found between spo-SBC and either CD-SBC or CrD-SBC. CD-SBC exhibited a significantly higher rate of MSI and median tumour-infiltrating lymphocytes [TIL] than CrD-SBC and spo-SBC. Among the whole SBC series, both MSI─which was the result of MLH1 promoter methylation in all but one cases─and high TIL density were associated with improved survival at univariable and stage-inclusive multivariable analysis. However, only TILs retained prognostic power when clinical subgroups were added to the multivariable model. KRAS mutation and HER2 amplification were detected in 30% and 7% of cases, respectively, without prognostic implications. Conclusions In comparison with CrD-SBC, CD-SBC patients harbour MSI and high TILs more frequently and show better outcome. This seems mainly due to their higher TIL density, which at multivariable analysis showed an independent prognostic value. MSI/TIL status, KRAS mutations and HER2 amplification might help in stratifying patients for targeted anti-cancer therapy.
- Published
- 2017
29. Genistein antagonizes gliadin-induced CFTR malfunction in models of celiac disease
- Author
-
Marco Silano, Guido Kroemer, Valeria Raia, Manuela D’Eletto, Alice Castaldo, Mauro Piacentini, Romina Monzani, Gianni Bona, Antonella Tosco, Eleonora Ferrari, Speranza Esposito, Luigina Romani, Valeria Rachela Villella, Gian Luigi Marseglia, Alessandro Luciani, Luigi Maiuri, Federica Rossin, Esposito, S, Villella, Vr, Ferrari, E, Monzani, R, Tosco, A, Rossin, F, D'Eletto, M, Castaldo, A, Luciani, A, Silano, M, Bona, G, Marseglia, Gl, Romani, L, Piacentini, M, Raia, V, Kroemer, G, and Maiuri, L
- Subjects
Male ,Aging ,Cystic Fibrosis Transmembrane Conductance Regulator ,Genistein ,Gliadin ,CFTR ,celiac disease ,genistein ,gluten peptides ,inflammation ,Ivacaftor ,Gene Knockout Techniques ,Mice ,chemistry.chemical_compound ,Gluten peptides ,Celiac disease ,Intestinal Mucosa ,Mice, Inbred BALB C ,biology ,Chemistry ,food and beverages ,Inflammation ,Cell biology ,Female ,medicine.symptom ,Protein Binding ,Research Paper ,medicine.drug ,congenital, hereditary, and neonatal diseases and abnormalities ,Settore BIO/06 ,Models, Biological ,digestive system ,Peripheral blood mononuclear cell ,Interferon-gamma ,Antigen ,Cell surface receptor ,medicine ,Animals ,Humans ,nutritional and metabolic diseases ,Cell Biology ,Potentiator ,Peptide Fragments ,digestive system diseases ,Disease Models, Animal ,coeliac disease genistein CFTR ,Dietary Supplements ,biology.protein ,Caco-2 Cells - Abstract
In celiac disease (CD), an intolerance to dietary gluten/gliadin, antigenic gliadin peptides trigger an HLA-DQ2/DQ8-restricted adaptive Th1 immune response. Epithelial stress, induced by other non-antigenic gliadin peptides, is required for gliadin to become fully immunogenic. We found that cystic-fibrosis-transmembrane-conductance-regulator (CFTR) acts as membrane receptor for gliadin-derived peptide P31-43, as it binds to CFTR and impairs its channel function. P31-43-induced CFTR malfunction generates epithelial stress and intestinal inflammation. Maintaining CFTR in an active open conformation by the CFTR potentiators VX-770 (Ivacaftor) or Vrx-532, prevents P31-43 binding to CFTR and controls gliadin-induced manifestations. Here, we evaluated the possibility that the over-the-counter nutraceutical genistein, known to potentiate CFTR function, would allow to control gliadin-induced alterations. We demonstrated that pre-treatment with genistein prevented P31-43-induced CFTR malfunction and an epithelial stress response in Caco-2 cells. These effects were abrogated when the CFTR gene was knocked out by CRISP/Cas9 technology, indicating that genistein protects intestinal epithelial cells by potentiating CFTR function. Notably, genistein protected gliadin-sensitive mice from intestinal CFTR malfunction and gliadin-induced inflammation as it prevented gliadin-induced IFN-γ production by celiac peripheral-blood-mononuclear-cells (PBMC) cultured ex-vivo in the presence of P31-43-challenged Caco-2 cells. Our results indicate that natural compounds capable to increase CFTR channel gating might be used for the treatment of CD.
- Published
- 2019
30. Targeting autophagy as a novel strategy for facilitating the therapeutic action of potentiators on ΔF508 cystic fibrosis transmembrane conductance regulator
- Author
-
Guido Kroemer, Massimo Pettoello-Mantovani, Marco Silano, Bob J. Scholte, Ilaria Russo, Stefano Guido, Valeria Raia, Antonella De Matteis, Valeria Rachela Villella, Manuela Gavina, Alessandro Luciani, Rosa Carnuccio, Luigi Maiuri, Maria Chiara Maiuri, Speranza Esposito, Alberto Luini, Luciani, A, Villella, Vr, Esposito, S, Gavina, M, Russo, I, Silano, M, Guido, Stefano, Pettoello Mantovani, M, Carnuccio, Rosa, Scholte, B, DE MATTEIS, Maria Antonietta, Maiuri, MARIA CHIARA, Raia, Valeria, Luini, A, Kroemer, G, Maiuri, L., and Cell biology
- Subjects
Lipopolysaccharides ,Male ,Cystic Fibrosis ,Cystic Fibrosis Transmembrane Conductance Regulator ,Pharmacology ,Cystic fibrosis ,Antioxidants ,Epithelium ,Mice ,chemistry.chemical_compound ,Sequestosome-1 Protein ,Autophagy ,CFTR potentiators ,Cystamine ,Therapy ,Adaptor Proteins, Signal Transducing ,Adolescent ,Animals ,Apoptosis Regulatory Proteins ,Beclin-1 ,Cell Membrane ,Child ,Epithelial Cells ,Female ,Genistein ,Heat-Shock Proteins ,Humans ,Inflammation ,Lung ,Membrane Proteins ,Nasal Mucosa ,Nasal Polyps ,Organometallic Compounds ,Salicylates ,Molecular Targeted Therapy ,Molecular Biology ,Cell Biology ,biology ,Adaptor Proteins ,BECN1 ,respiratory system ,Cystic fibrosis transmembrane conductance regulator ,Translational Research Paper ,congenital, hereditary, and neonatal diseases and abnormalities ,medicine ,Protein Glutamine gamma Glutamyltransferase 2 ,ΔF508 ,Signal Transducing ,Potentiator ,medicine.disease ,digestive system diseases ,respiratory tract diseases ,chemistry ,Immunology ,biology.protein ,Ex vivo - Abstract
Channel activators (potentiators) of cystic fibrosis (CF) transmembrane conductance regulator (CFTR), can be used for the treatment of the small subset of CF patients that carry plasma membrane-resident CFTR mutants. However, approximately 90% of CF patients carry the misfolded Delta F508-CFTR and are poorly responsive to potentiators, because Delta F508-CFTR is intrinsically unstable at the plasma membrane (PM) even if rescued by pharmacological correctors. We have demonstrated that human and mouse CF airways are autophagy deficient due to functional sequestration of BECN1 and that the tissue transglutaminase-2 inhibitor, cystamine, or antioxidants restore BECN1-dependent autophagy and reduce SQSTM1/p62 levels, thus favoring Delta F508-CFTR trafficking to the epithelial surface. Here, we investigated whether these treatments could facilitate the beneficial action of potentiators on Delta F508-CFTR homozygous airways. Cystamine or the superoxide dismutase (SO D)/catalase-mimetic EUK-134 stabilized Delta F508-CFTR at the plasma membrane of airway epithelial cells and sustained the expression of CFTR at the epithelial surface well beyond drug withdrawal, overexpressing BECN1 and depleting SQSTM1. This facilitates the beneficial action of potentiators in controlling inflammation in ex vivo Delta F508-CFTR homozygous human nasal biopsies and in vivo in mouse Delta F508-CFTR lungs. Direct depletion of Sqstm1 by shRNAs in vivo in Delta F508-CFTR mice synergized with potentiators in sustaining surface CFTR expression and suppressing inflammation. Cystamine pre-treatment restored Delta F508-CFTR response to the CFTR potentiators genistein, Vrx-532 or Vrx-770 in freshly isolated brushed nasal epithelial cells from Delta F508-CFTR homozygous patients. These findings delineate a novel therapeutic strategy for the treatment of CF patients with the Delta F508-CFTR mutation in which patients are first treated with cystamine and subsequently pulsed with CFTR potentiators.
- Published
- 2012
31. Low risk of colon cancer in patients with celiac disease
- Author
-
Volta, Umberto, Vincentini, Olimpia, Quintarelli, Federica, Felli, Cristina, Silano, Marco, Gasbarrini, G, De Vitis, V, Santini, D, Scaggiante, F, Castellano, E, Grosso, S, Campanella, J, Corazza, GR, Sandri, G, Giorgetti, G, Caio, G, Lo Perfido, S, Perri F, Festa V, Pelli MA, Cavalletti ML, Segato S, Curzio M, Pennazio M, Rossini FP, Picarelli A, Pera A, Ercole E, Passaleva MT, Barbato M, Usai P, Dore MF, Chilovi F, Piazzi L, Zancanella L, Boarino V, Ferrari A., GRECO, LUIGI, AURICCHIO, SALVATORE, Volta, Umberto, Vincentini, Olimpia, Quintarelli, Federica, Felli, Cristina, Silano, Marco, Gasbarrini, G, De Vitis, V, Greco, Luigi, Auricchio, Salvatore, Santini, D, Scaggiante, F, Castellano, E, Grosso, S, Campanella, J, Corazza, Gr, Sandri, G, Giorgetti, G, Caio, G, Lo, Perfido, S, Perri, F, Festa, V, Pelli, Ma, Cavalletti, Ml, Segato, S, Curzio, M, Pennazio, M, Rossini, Fp, Picarelli, A, Pera, A, Ercole, E, Passaleva, Mt, Barbato, M, Usai, P, Dore, Mf, Chilovi, F, Piazzi, L, Zancanella, L, Boarino, V, Ferrari, A., Volta U, Vincentini O, Quintarelli F, Felli C, Silano M, and Collaborating Centres of the Italian Registry of the Complications of Celiac Disease
- Subjects
Male ,Colorectal cancer ,COLON CANCER ,Disease ,Gastroenterology ,Colon carcinoma ,Retrospective Studie ,Medicine ,Child ,Colonic Neoplasm ,education.field_of_study ,Medicine (all) ,Incidence ,Celiac disease ,Gluten-free diet ,Adolescent ,Adult ,Carcinoma ,Celiac Disease ,Child, Preschool ,Colonic Neoplasms ,Diet, Gluten-Free ,Female ,Follow-Up Studies ,Humans ,Infant ,Infant, Newborn ,Italy ,Middle Aged ,Patient Compliance ,Retrospective Studies ,Risk Assessment ,Young Adult ,Population study ,Human ,Cohort study ,medicine.medical_specialty ,Population ,Follow-Up Studie ,NO ,Internal medicine ,In patient ,Preschool ,education ,business.industry ,Newborn ,medicine.disease ,digestive system diseases ,Diet ,Standardized mortality ratio ,Gluten-Free ,Celiac disease, colon carcinoma, gluten-free diet ,business - Abstract
Objective. Celiac disease (CD) has strongly been established as associated with some site-specific gastrointestinal malignancies. On the contrary, according to the few reports available, the risk of colon carcinoma in CD patients has been described similar to that of general population. In this cohort study, we describe the risk of colon carcinoma in a group of Italian celiac patients. Materials and methods. The study population included all CD patients diagnosed at the Collaborating Centers of the Italian Registry of CD between 1st January 1982 and 31st December 2006. Upon diagnosis of CD and upon at every subsequent clinical control, the Collaborating Centers filled in a validated form for each CD patient reporting information about demographic data, possible occurrence of a neoplasm and adherence to a gluten-free diet. Results. Out of 1757 celiac patients enrolled, 6 developed a colon carcinoma during the follow-up period (mean: 18.1 years). The standardized incidence ratio (SIR) resulted 0.29 (95% CI = 0.07–0.45). Stratifying the risk for the dietary gluten intake, the SIR dropped to 0.07 (95% CI = 0.009–0.27) for CD patients with a strict adherence to a gluten-free diet. Conclusion. We confirm the previous finding that there is low risk to develop a colon cancer in celiac patients.
- Published
- 2014
32. Low-molecular-weight heparins induce decidual heparin-binding epidermal growth factor-like growth factor expression and promote survival of decidual cells undergoing apoptosis.
- Author
-
Di Simone N, Di Nicuolo F, Castellani R, Veglia M, Tersigni C, Silano M, Tritarelli A, Scambia G, Marana R, Di Simone, Nicoletta, Di Nicuolo, Fiorella, Castellani, Roberta, Veglia, Manuela, Tersigni, Chiara, Silano, Marco, Tritarelli, Alessandra, Scambia, Giovanni, and Marana, Riccardo
- Abstract
Objective: To evaluate the effects of low-molecular-weight heparins (LMWHs) on decidual heparin-binding epidermal growth factor-like growth factor (HB-EGF) expression/secretion and on TNF-α-induced decidual apoptosis.Design: Experimental study.Setting: Department of Obstetrics and Gynecology, Università Cattolica del Sacro Cuore, Rome, Italy.Patient(s): Cultures of primary decidual cells isolated from human term placenta.Intervention(s): The effects of LMWHs (tinzaparin and enoxaparin) on decidual HB-EGF expression and secretion were investigated by Western blot analysis and ELISA, respectively. TNF-α-induced decidual apoptosis was evaluated by annexin V staining, terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) assay, and caspase activities.Main Outcome Measure(s): Decidual HB-EGF expression/secretion and apoptotic rate induced by TNF-α were investigated.Result(s): Tinzaparin enhanced decidual HB-EGF expression and secretion. TNF-α reduced the number of viable cells by inducing apoptosis. Simultaneous addition of LMWHs (primarily tinzaparin) blocked the increase in annexin V- and TUNEL-positive cells and reduced the amount of caspase activities.Conclusion(s): Both LMWHs induced a significant increase in decidual HB-EGF expression/secretion and reduced TNF-α-induced decidual apoptosis. Tinzaparin demonstrated higher efficacy. [ABSTRACT FROM AUTHOR]- Published
- 2012
- Full Text
- View/download PDF
33. Analysis of suspected adverse reactions to food supplements containing beehive products: an update from the Italian Phytovigilance System.
- Author
-
Ippoliti I, Di Giacomo S, Mazzanti G, Silano M, and Menniti-Ippolito F
- Subjects
- Humans, Italy, Female, Male, Middle Aged, Adult, Aged, Young Adult, Adolescent, Pharmacovigilance, Adverse Drug Reaction Reporting Systems, Allergens adverse effects, Child, Dietary Supplements adverse effects
- Abstract
Introduction: Beehive products are widely used in food supplements; however, their composition variability and allergenic components have raised some concerns. This work aims to provide information about the beehive products safety profile by evaluating the suspected adverse reactions (ARs)., Methods: The suspected report of ARs collected within the Italian Phytovigilance System (IPS) were evaluated. The clinical and demographic characteristics of the cases were described, and the causality assessment performed., Results: 61 reports were analysed, mainly concerned women. Serious events were reported in 17 forms (28%). The ARs (n=116) referred to respiratory (25.0%), skin (24.1%), and gastrointestinal disorders (21.5%). Label warnings for atopic subjects were present only in 7 food supplements. The causality assessment was mostly probable (54.1%)., Conclusions: Present findings outline relevant information about the safety issues of beehive product consumption, especially in atopic or allergic subjects, and strengthen the importance of IPS to point out safety signals.
- Published
- 2024
- Full Text
- View/download PDF
34. Adherence to Mediterranean Diet and its main determinants in a sample of Italian adults: results from the ARIANNA cross-sectional survey.
- Author
-
Cardamone E, Iacoponi F, Di Benedetto R, Lorenzoni G, Di Nucci A, Zobec F, Gregori D, and Silano M
- Abstract
Introduction: Over the last years, many Mediterranean countries, including Italy, have witnessed a shift away from the Mediterranean Diet, thus contributing to the high rates of overweight and obesity. The survey "Adherence to Mediterranean Diet in Italy (ARIANNA)" aimed to evaluate the Adherence to Mediterranean Diet (AMD) and its main determinants in the Italian population., Materials and Methods: This study started on March 2023 and was addressed to adults aged ≥17 years, born and resident in Italy, proficient in Italian. Data are collected electronically through a voluntary, anonymous and self-administered questionnaire on the project website. Univariate and then multivariate logistic regressions were performed to evaluate associations between AMD and demographic characteristics, socio-economic status, health status, and lifestyle., Results: On a total of 3,732 completed questionnaires, the 87.70% of the respondents was female and the 71.28% was 17-40 years old. The 83.82% of the respondents had medium AMD, 11.33% low and only 4.85% high. The multivariate analysis revealed that being male ( p < 0.001), aged >40 years ( p < 0.05), workers ( p ≤ 0.001), and unemployed ( p < 0.05), determined the probability of having a lower AMD. Vegans and vegetarian's diets positively contributed to a higher AMD ( p < 0.001)., Discussion: These results highlighted a medium AMD in the Italian adult participants and suggested the necessity to implement tailored public health intervention strategies to improve food habits., Competing Interests: FZ works at Zeta Research Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Cardamone, Iacoponi, Di Benedetto, Lorenzoni, Di Nucci, Zobec, Gregori and Silano.)
- Published
- 2024
- Full Text
- View/download PDF
35. Nutrition, Physical Activity and Smoking Habit in the Italian General Adult Population: CUORE Project Health Examination Survey 2018-2019.
- Author
-
Donfrancesco C, Buttari B, Marcozzi B, Sieri S, Di Lonardo A, Lo Noce C, Profumo E, Vespasiano F, Agnoli C, Vannucchi S, Silano M, Galeone D, Bellisario P, Vaia F, and Palmieri L
- Abstract
Background: Tobacco consumption, incorrect nutrition and insufficient physical activity/sedentariness represent modifiable NCDs risk factors in Western countries. To evaluate recent lifestyle indicators in Italy, data from the national Health Examination Survey (HES), implemented in 2018-2019 within the CUORE Project, were assessed., Methods: Age-sex standardized results from random samples of Italian general population (35-74 years) were reported by sex, age-class, educational level and geographical area. From 2106 participants, 2090 were considered for smoking habit, 2016 for physical activity and 1578 for nutrition. Standardized questionnaires were used for smoking habit and physical activity, and the EPIC questionnaire for nutrition., Results: Total cigarette current smokers were 23% in men and 19% in women; sedentariness during leisure time was 34% in men and 45% in women and at work 45% and 47% in men and women, respectively. Prevalence of balanced eating behaviours for vegetables was 28% in men and 39% in women; and for fruits 50% and 52%, respectively; prevalence of correct lifestyle (not smoker, regular physical activity and following at least five correct eating behaviours) was 7% and 12% for men and women, respectively., Conclusions: In 2018-2019, levels of unhealthy lifestyles were found to be still epidemic and basically stable compared to 10 years earlier (slight smoking habit decrease, slight sedentariness increase and slight nutrition improvements); intersectoral strategies and monitoring need to be continued.
- Published
- 2024
- Full Text
- View/download PDF
36. Tackling the challenge of cardiovascular diseases and diabetes across Europe: a joint action by more than 300 public health professionals. Commentary.
- Author
-
Armocida B, Formenti B, Silano M, Aszalos A, Bueno H, Elyamani M, Zabala AF, Leonardi M, Lindström J, Michalak K, Morillo B, Nowicki M, Oprešnik D, Palmieri L, Papa R, Peltonen M, Safadi H, Shchetynina H, Skogberg N, Tolonen H, Wouters E, Zaletel J, and Onder G
- Subjects
- Humans, Europe, Cardiovascular Diseases prevention & control, Cardiovascular Diseases therapy, Diabetes Mellitus therapy, Diabetes Mellitus epidemiology, Public Health
- Abstract
Cardiovascular diseases (CVD) and diabetes pose significant health challenges in Europe, affecting millions and burdening healthcare systems. The recent EU4Health Programme places reducing the burden of non-communicable diseases (NCD) at the forefront, through a Joint Action focused on CVD and diabetes (JACARDI, Joint Action on CARdiovascular diseases and DIabetes). This initiative unites 21 European countries, including Ukraine, and over 300 experts. Employing an innovative approach and standardised methodology, JACARDI implements 142 pilot projects covering the entire "patient" journey. Particular focus will be given to improvement of data availability and quality. Additionally, JACARDI will emphasise transversal and intersectional aspects, such as health equity, determinants of health, and social, cultural, and ethnic diversity, while pioneering gender-transformative leadership. Committed to evidence-based interventions, JACARDI aims to harmonise strategies and disseminate knowledge for enhanced CVD and diabetes prevention and management. The goal is to identify effective strategies for wider implementation, fostering cross-national collaboration and fortifying Europe's health resilience.
- Published
- 2024
- Full Text
- View/download PDF
37. Protein and growth during the first year of life: a systematic review and meta-analysis.
- Author
-
Milani GP, Edefonti V, De Cosmi V, Bettocchi S, Mazzocchi A, Silano M, Pietrobelli A, and Agostoni C
- Subjects
- Infant, Female, Humans, Breast Feeding, Milk, Human, Infant Nutritional Physiological Phenomena, Dietary Proteins, Infant Formula
- Abstract
Dietary protein intake in the first year of life might influence later growth. We conducted a systematic review to investigate the growth effects of interventions based on infant formula composition providing different amounts of protein within the first year of life of healthy term infants; in the absence of other comparable information over the investigated period, a meta-analysis further compared weight or length gain at 120 days from high- (>2.0 g/100 kcal) and low-protein (≤2.0 g/100 kcal) content formula groups. Twelve papers (n = 2275) were included and five of them (n = 677) contributed to the meta-analysis. Most studies compared a high-protein formula, a low-protein formula, and breastfeeding. Evidence from the systematic review was inconclusive due to heterogeneity in design and treatments. In the presence of modest heterogeneity but in the absence of publication bias, the weighted mean difference for weight gain at 120 days was -0.02 g/day (95% CI: -1.41, 1.45); with higher heterogeneity, the weighted MD estimate of length gain at 120 days was 0.004 cm/month (95% CI: -0.26, 0.27). Although limited and underpowered, evidence from the meta-analysis does not support the assumption that high- vs. low-protein content formulas during exclusive milk-feeding lead to different growth outcomes in the first months of life. Prospero registration number: CRD42017058535. IMPACT: The optimal amount of dietary protein that should be given to healthy full-term infants early in life is still debated. Despite heterogeneity in study design, treatments, and outcomes, this systematic review showed that there is no clear-cut effect on the growth of different amounts of protein intake from formulas or complementary feeding. Evidence from the meta-analysis based on the five articles enrolling infants <1 month of life does not support the previous assumption that high- vs. low-protein content formulas during exclusive milk-feeding lead to different growth outcomes in the first 4 months of life., (© 2023. The Author(s).)
- Published
- 2023
- Full Text
- View/download PDF
38. Role of Portion Size in the Context of a Healthy, Balanced Diet: A Case Study of European Countries.
- Author
-
Carruba MO, Ragni M, Ruocco C, Aliverti S, Silano M, Amico A, Vaccaro CM, Marangoni F, Valerio A, Poli A, and Nisoli E
- Subjects
- Humans, Diet, Healthy, Energy Intake, Vegetables, Europe, Diet, Portion Size, Eating
- Abstract
Over the past decades, a generalised increase in food portion sizes has probably contributed to the growing global obesity epidemic. Increasing awareness of appropriate portion sizes could contribute to reversing this trend through better control of calorie intake. In this study, a comparison of standard portion sizes in European countries for various food categories shows a wide variability of their importance for food, nutrient, and energy consumption according to government and institutional websites. On the other hand, the overall averages appear to be largely in line with the values indicated by the Italian Society of Human Nutrition, which is the most comprehensive and detailed document among those evaluated. The exceptions are milk and yoghurt, for which the reference portions in Europe are generally higher, and vegetables and legumes, for which portions are smaller than those reported in the Italian document. Moreover, the portion sizes of staple foods (e.g., pasta and potatoes) vary according to different food traditions. It is reasonable to consider that the creation of harmonised standard reference portions common to the European countries, based on international guidelines and scientific evidence, would significantly contribute to consumers' nutritional education and ability to make informed choices for a healthy diet.
- Published
- 2023
- Full Text
- View/download PDF
39. Adherence to Mediterranean diet in Italy (ARIANNA) cross-sectional survey: study protocol.
- Author
-
Cardamone E, Di Benedetto R, Lorenzoni G, Gallipoli S, Ghidina M, Zobec F, Iacoponi F, Gregori D, and Silano M
- Subjects
- Adolescent, Child, Female, Male, Humans, Cross-Sectional Studies, Italy, Administrative Personnel, Ethics Committees, Review Literature as Topic, Diet, Mediterranean
- Abstract
Introduction: There is evidence, although limited, that the Italian population has been no longer following a Mediterranean dietary pattern. The ARIANNA (Adherence to the Mediterranean Diet in Italy) project consists of a survey-based cross-sectional study with the objective of gaining a greater knowledge of adherence to the Mediterranean Diet and its main determinants in different age groups of the Italian population., Methods/analysis: The ARIANNA study will involve males and females aged ≥7 years, born and resident in Italy, and proficient in Italian. The voluntary enrolment will be in the period between March 2023 and May 2023. The data, which will include sociodemographic factors and dietary habits, will be collected through a web-based questionnaire. Adherence to the Mediterranean Diet will be assessed through the use of two validated score systems: the Mediterranean Diet Quality Index in children and adolescents for participants aged ≤16 years and the Mediterranean Diet Serving Score for participants aged ≥17 years. A review of the scientific literature will be carried out to collect historical data on adherence to the Mediterranean dietary pattern in the Italian population, which will be compared with those collected within this project., Ethics and Dissemination: The ARIANNA study has been approved by the Ethics Committee of Istituto Superiore di Sanità. The results will be disseminated through peer-reviewed papers, leaflets and documents for the general public. A report will be presented to the national policy makers, to give them the tools to implement appropriate intervention to improve, in necessary, the adherence to Mediterranean dietary pattern in Italy., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
- Published
- 2023
- Full Text
- View/download PDF
40. Evaluation of the Potential Anti-Inflammatory Activity of Black Rice in the Framework of Celiac Disease.
- Author
-
Piazza S, Colombo F, Bani C, Fumagalli M, Vincentini O, Sangiovanni E, Martinelli G, Biella S, Silano M, Restani P, Dell'Agli M, and Di Lorenzo C
- Abstract
Inflammation and oxidative stress are two mechanisms involved in the pathogenesis of celiac disease (CD). Since the direct effect of gliadin on the intestinal epithelia is less studied, the aims of this study were the development of a specific cellular model based on the use of gliadin as a pro-inflammatory stimulus and the evaluation of the potential antioxidant and anti-inflammatory properties of extracts from different black rice in the framework of CD. The rice extracts were in vitro digested, characterized in terms of phenolic compounds and antioxidant capacity, and tested on Caco-2 cells to investigate their inhibitory effect on Reactive Oxygen Species, the NF-κB transcription and the CXC chemokines (sICAM-1, IL-8, and CXCL-10). In addition, the role of the extracts in modulating the activation of epithelial cells in CD was confirmed by applying the K562(S) agglutination test. The black rice extracts showed inhibitory effects on the production of the oxidative and the inflammatory mediators considered, with particular reference to lymphocyte-attracting CXCL-10 both before and after digestion. The presence of anthocyanins and their digestion metabolites may account for the observed anti-inflammatory activity after in vitro digestion. This work provided preliminary data supporting the use of black rice as a healthy food or ingredient of food supplements for celiacs., Competing Interests: The authors declare no conflict of interest.
- Published
- 2022
- Full Text
- View/download PDF
41. Policy implementation and priorities to create healthy food environments using the Healthy Food Environment Policy Index (Food-EPI): A pooled level analysis across eleven European countries.
- Author
-
Pineda E, Poelman MP, Aaspõllu A, Bica M, Bouzas C, Carrano E, De Miguel-Etayo P, Djojosoeparto S, Blenkuš MG, Graca P, Geffert K, Hebestreit A, Helldan A, Henjum S, Huseby CS, Gregório MJ, Kamphuis C, Laatikainen T, Løvhaug AL, Leydon C, Luszczynska A, Mäki P, Martínez JA, Raulio S, Romaniuk P, Roos G, Salvador C, Sassi F, Silano M, Sotlar I, Specchia ML, de Arriaga MT, Terragni L, Torheim LE, Tur JA, von Philipsborn P, Harrington JM, and Vandevijvere S
- Abstract
Background: Food environments have been recognised as highly influential on population diets. Government policies have great potential to create healthy food environments to promote healthy diets. This study aimed to evaluate food environment policy implementation in European countries and identify priority actions for governments to create healthy food environments., Methods: The Healthy Food Environment Policy Index (Food-EPI) was used to evaluate the level of food environment policy and infrastructure support implementation in Estonia, Finland, Germany, Ireland, Italy, the Netherlands, Norway, Poland, Portugal, Slovenia, and Spain in 2019-2021. Evidence of implementation of food environment policies was compiled in each country and validated by government officials. National experts evaluated the implementation of policies and identified priority recommendations., Findings: Finland had the highest proportion (32%, n = 7/22) of policies shaping food environments with a "high" level of implementation. Slovenia and Poland had the highest proportion of policies rated at very low implementation (42%, n = 10/24 and 36%, n = 9/25 respectively). Policies regarding food provision, promotion, retail, funding, monitoring, and health in all policies were identified as the most important gaps across the European countries. Experts recommended immediate action on setting standards for nutrients of concern in processed foods, improvement of school food environments, fruit and vegetable subsidies, unhealthy food and beverage taxation, and restrictions on unhealthy food marketing to children., Interpretation: Immediate implementation of policies and infrastructure support that prioritize action towards healthy food environments is urgently required to tackle the burden of obesity and diet-related non-communicable diseases in Europe., Funding: This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 774548 and from the Joint Programming Initiative "A Healthy Diet for a Healthy Life"., Competing Interests: No conflicts of interests were declared., (© 2022 The Author(s).)
- Published
- 2022
- Full Text
- View/download PDF
42. Impact of the COVID-19 lockdown on psychological health and nutritional habits in Italy: results from the #PRESTOinsieme study.
- Author
-
Lorenzoni G, Azzolina D, Maresio E, Gallipoli S, Ghidina M, Baldas S, Berchialla P, Giron MC, Silano M, and Gregori D
- Subjects
- Anxiety epidemiology, Communicable Disease Control, Cross-Sectional Studies, Depression epidemiology, Female, Habits, Humans, Italy epidemiology, SARS-CoV-2, Stress, Psychological epidemiology, Surveys and Questionnaires, COVID-19 epidemiology, COVID-19 prevention & control
- Abstract
Objectives: The present work aims to present the results of the ' PRESTOinsieme ' (which is ' we will be together soon ' in English). The web-based survey (www.prestoinsieme.com) describes changes in lifestyle habits and symptoms of psychological discomfort in the Italian population during the COVID-19 lockdown., Design: Cross-sectional online survey disseminated by messaging apps (ie, WhatsApp and Telegram) and social networks (ie, Instagram, Facebook and LinkedIn)., Setting: Italy., Participants: Italian population older than 16 years of age., Exposure: COVID-19 lockdown., Main Outcomes and Measures: Survey respondents filled out a set of validated questionnaires aimed at assessing lifestyle habits and psychological health, that is, the General Health Questionnaire (GHQ-12) to screen for psychological distress, the Impact of Event Scale-Revised (IES-R) to screen for post-traumatic stress and the Center for Epidemiologic Studies Depression Scale (CES-D)., Results: Survey respondents totalled 5008. Moderate or severe psychological distress was reported in 25.5% and 22% of survey respondents, respectively. Lower age, female gender, being unemployed (OR 1.57, 95% CI 1.22 to 2.02) or being a student (OR 1.73, 95% CI 1.31 to 2.28) were predictors of more severe depressive symptoms., Conclusions: The present study is one of the largest population-based surveys conducted in Italy during the first COVID-19 lockdown, providing valuable data about the Italian population's psychological health. Further studies should be conducted to understand whether psychological distress persists after the end of the lockdown., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
- Published
- 2022
- Full Text
- View/download PDF
43. Circulating microRNAs as novel non-invasive biomarkers of paediatric celiac disease and adherence to gluten-free diet.
- Author
-
Felli C, Baldassarre A, Uva P, Alisi A, Cangelosi D, Ancinelli M, Caruso M, Paolini A, Montano A, Silano M, Vincentini O, Catassi C, Lionetti E, Gatti S, Ferretti F, and Masotti A
- Subjects
- Biomarkers, Child, Diet, Gluten-Free, Humans, Celiac Disease diagnosis, Celiac Disease genetics, Circulating MicroRNA, MicroRNAs genetics
- Abstract
Background: Celiac Disease (CD) is a multifactorial autoimmune enteropathy (with a prevalence of approximately 1% worldwide) that exhibits a wide spectrum of clinical, serological and histological manifestations. For the diagnosis of paediatric CD, the gold standard is the combination of serological tests (with high TGA-IgA values greater than 10 times the upper limit of normal) and duodenal biopsy (with a positive TGA-IgA but low titer). Therefore, a diagnostic test that totally excludes an invasive approach has not been discovered so far and the discovery of novel biological markers would represent an undoubted advantage for the diagnosis of CD and prognostic evaluation. MicroRNAs (miRNAs), small non-coding RNAs (18-22 nucleotides) that regulate gene expression at post-transcriptional level and play important roles in many biological processes, represent a novel class of potential disease biomarkers. Their presence in biological fluids (i.e., serum, plasma, saliva, urine) provides the opportunity to employ circulating miRNAs as novel non-invasive biomarkers., Methods: In our prospective observational study, we examined the expression of circulating miRNAs in a cohort of CD patients (both at diagnosis and on gluten-free diet, respectively referred as CD and GFD) compared to healthy controls. By small RNA-Seq we discovered a set of circulating miRNAs that were further validated by qPCR with specific assays., Findings: We found that out of the 13 miRNAs able to discriminate the three groups (i.e., CD, GFD and controls), three of them, namely miR-192-5p, miR-215-5p and miR-125b-5p (alone or in combination), were able to discriminate these three groups with high accuracy and specificity., Interpretation: Our conclusions emphasize that these circulating miRNAs can be employed not only for the diagnosis of CD patients with a low TGA-IgA titer but also to monitor the adherence to a gluten-free diet by CD patients. In conclusion, we suggest the use of the circulating miRNAs identified in this work as a novel diagnostic and follow-up tool for paediatric CD., Funding: This work was supported by Fondazione Celiachia Onlus (FC) Grant n° 018/FC/2013 and by Italian Ministry of Health (Ricerca Corrente)., Competing Interests: Declaration of interests All of the authors declare no conflict of interest., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
44. Implementation level of best practice policies by Italian Government for healthier food environments: Healthy Food Environment Policy Index (Food-EPI).
- Author
-
Zaçe D, Di Pilla A, Silano M, Carini E, Cacciatore P, Frisicale EM, Vandevijvere S, Sassi F, and Specchia ML
- Subjects
- Food, Humans, Italy, Marketing, Government, Policy
- Abstract
Background: This work aims to identify policies implemented for healthy food environments in Italy within The Healthy Food Environment Policy Index (Food-EPI) project., Methods: Food-EPI tool, which includes two components, thirteen domains and fifty good practice indicators, was adapted for the Italian context. Evidence for implementation was gathered and summarized for all fifty indicators from data sources such as governmental websites, non-government organizations publications and websites and via direct contact with Government officials., Results: The highest level of evidence was found within five domains: food composition (2/2 indicators), labelling (3/4), promotion (4/5), provision (4/5) and leadership (5/5). The domains with less identified evidence were food prices (1/4), food in retail (0/4), trade and investment (0/2) and platforms and interaction (1/4)., Conclusions: The evidence summarization and the upcoming stakeholders' meeting to rate the level of implementation for each indicator in Italy have the potential to improve Government commitment to shape healthier food environments.
- Published
- 2022
- Full Text
- View/download PDF
45. Pigmented Corn Varieties as Functional Ingredients for Gluten-Free Products.
- Author
-
Colombo F, Di Lorenzo C, Petroni K, Silano M, Pilu R, Falletta E, Biella S, and Restani P
- Abstract
Oxidative stress, one among the several factors responsible for the gluten toxicity in celiac disease, together with inflammation and duodenal mucosal injury, are only partially reduced by the gluten-free diet. Thanks to their phenolic profile, the pigmented varieties of corn could be an interesting source of dietary antioxidants for the formulation of new gluten-free ingredients. The aim of this research was: (1) to characterize the phenolic profile and the associated antioxidant properties of corn samples with different pigmentation, using spectrophotometric and chromatographic techniques and (2) to assess the stability of anthocyanins during the gastro-intestinal digestion. The pigmented varieties showed a significantly higher content of polyphenols compared to the common yellow varieties and, as a consequence, a higher antioxidant activity. Although corn is among the cereals most frequently used in gluten-free products, it can produce an inflammatory response in some celiac patients. Therefore, after the chemical characterization, the safety of the pigmented varieties for celiac patients was confirmed using different in vitro models (cell agglutination test and the measure of transepithelial electrical resistance). Although in vivo studies are necessary, the data collected in this study underline that the pigmented corn could have a role in reducing the oxidative stress at the intestinal level in celiac subjects.
- Published
- 2021
- Full Text
- View/download PDF
46. What Is the Nutritional Composition of Ultra-Processed Food Marketed in Italy?
- Author
-
Lorenzoni G, Di Benedetto R, Silano M, and Gregori D
- Subjects
- Consumer Behavior, Humans, Italy, Direct-to-Consumer Advertising, Fast Foods analysis, Food Handling, Food Labeling, Nutritive Value, Recommended Dietary Allowances
- Abstract
The present study aimed to provide a descriptive analysis of the nutrient profile of ultra-processed foods (UPFs) marketed in Italy according to three front-of-pack labeling (FOPL) schemes implemented by France, i.e., the Nutriscore; by the United Kingdom, i.e., Multiple Traffic Lights (MTL); and by Italy, i.e., the NutrInform battery. The analysis was made in fourteen food product categories, corresponding to 124 foods. The application of the Nutriscore scheme showed that a significant proportion of foods (23%) were awarded an A or B. Furthermore, the analysis according to the MTL showed that food products that were above the threshold ("red") for fat, saturated fats, sugars, and salt ranged from 13% to 31%. Interestingly, even though all foods considered in the analysis were UPF, they were heterogeneous in nutritional composition, as demonstrated by the FOPL schemes applied, showing that UPF represent a heterogeneous group of foods with different characteristics. Such a finding may have relevant implications for epidemiological studies that analyze the association between UPF consumption and health outcomes, suggesting the need for better characterization of the effects of UPF intake on human health.
- Published
- 2021
- Full Text
- View/download PDF
47. Psychological Effects of Lockdown Measures for the COVID-19 Outbreak in Patients with Systemic Lupus Erythematosus.
- Author
-
Quartuccio L, De Marchi G, Azzolina D, Maresio E, Colatutto D, Binutti M, Monte M, Gallipoli S, Zobec F, Lanera C, Lorenzoni G, Palese A, Silano M, De Vita S, and Gregori D
- Abstract
Objective: To compare the psychological impact of the lockdown measures contrasting the COVID-19 outbreak between systemic lupus erythematosus (SLE) and general population., Patients and Methods: From July 15th to August 15th 2020, a retrospective survey referring to the period March 9th to May 18th 2020 was administered to SLE patients and the results of the survey, called LEPRE (Lupus Erythematosus PREsto) study, were compared with those from the PRESTO (imPact of quaRantine mEasures againST cOvid19) project, the same survey provided to the general population. Consecutive patients >18 years old affected by SLE and regularly followed in a single rheumatologic centre were involved. Primary outcome was to compare the scores of the Impact of Events Scale-Revised (IES-R), the General Health Questionnaire 12 (GHQ-12) and the Center for Epidemiological Depression Scale (CES-D) between patients and general population., Results: A total of 64 patients completed the survey. After a propensity score matching, they were compared to 128 people from PRESTO project. The median age among patients was 43 years (I-III interquartile range 35-54.5), 88% were female and 100% Caucasian. IES-R [(score>23: 57% (34) vs 49% (58)], GHQ-12 [(score>13: 85% (52) vs 88% (106)], and CES-D [(score>15: 45% (28) vs 40% (46)] scores were not statistically different between patients and controls (p>0.05)., Conclusion: Restrictive measures for COVID-19 pandemic had no greater impact on patients with SLE than in the general population. Strategy for coping to the SLE might be useful during lockdown measures and may be helpful for other chronic conditions., Competing Interests: No authors have any financial support or other benefits from commercial sources for the work reported on in the manuscript, or any other financial interests which could create a potential conflict of interest or the appearance of a conflict of interest concerning the work. The authors reported no conflicts of interest for this work., (© 2021 Quartuccio et al.)
- Published
- 2021
- Full Text
- View/download PDF
48. Screening for celiac disease among the personnel in active service of an Italian Armed Force.
- Author
-
Panici M, Vincentini O, Ciardi S, De Vitis I, Ricci S, Bartoccioni E, and Silano M
- Subjects
- Adolescent, Adult, Cohort Studies, Humans, Mass Screening, Middle Aged, Prevalence, Young Adult, Celiac Disease diagnosis, Celiac Disease epidemiology, Military Personnel
- Abstract
Introduction and Objective: Celiac disease (CD) affects the 1% of the general population worldwide. Because of its clinical variability, roughly the 70% of CD patients are not correctly diagnosed and not adequately treated. Active military personnel represent an interesting cohort for a CD screening. Upon the enrollment in the Armed Forces, a complete health check is carried out to exclude any diseases. Aim of the present work is to assess the CD prevalence among the personnel of Carabinieri Corps, an Italian armed force, through a serological screening., Results and Discussion: Out of 291 militaries (281 M, 10 F age range: 18.2-61.5) enrolled, 2 resulted affected by CD (prevalence: 0.7%); 1 to have high serological anti-TG and EMA level without duodenal mucosal lesions and 1 to have high serological anti-TG, but not EMA., Conclusion: These results show that the CD prevalence among a cohort of Italian militaries is similar to that of the general population.
- Published
- 2021
- Full Text
- View/download PDF
49. Personalized nutrition approach in pediatrics: a narrative review.
- Author
-
Milani GP, Silano M, Mazzocchi A, Bettocchi S, De Cosmi V, and Agostoni C
- Subjects
- Child, Energy Intake, Energy Metabolism, Feeding Behavior, Humans, Machine Learning, Metabolomics, Neural Networks, Computer, Nutrigenomics, Nutritional Status, Nutritive Value, Pediatric Obesity epidemiology, Pediatric Obesity physiopathology, Therapy, Computer-Assisted, Child Nutritional Physiological Phenomena, Diet adverse effects, Nutrition Therapy, Pediatric Obesity prevention & control, Pediatrics, Precision Medicine
- Abstract
Dietary habits represent the main determinant of health. Although extensive research has been conducted to modify unhealthy dietary behaviors across the lifespan, obesity and obesity-associated comorbidities are increasingly observed worldwide. Individually tailored interventions are nowadays considered a promising frontier for nutritional research. In this narrative review, the technologies of importance in a pediatric clinical setting are discussed. The first determinant of the dietary balance is represented by energy intakes matching individual needs. Most emerging studies highlight the opportunity to reconsider the widely used prediction equations of resting energy expenditure. Artificial Neural Network approaches may help to disentangle the role of single contributors to energy expenditure. Artificial intelligence is also useful in the prediction of the glycemic response, based on the individual microbiome. Other factors further concurring to define individually tailored nutritional needs are metabolomics and nutrigenomic. Since most available data come from studies in adult groups, new efforts should now be addressed to integrate all these aspects to develop comprehensive and-above all-effective interventions for children. IMPACT: Personalized dietary advice, specific to individuals, should be more effective in the prevention of chronic diseases than general recommendations about diet. Artificial Neural Networks algorithms are technologies of importance in a pediatric setting that may help practitioners to provide personalized nutrition. Other approaches to personalized nutrition, while promising in adults and for basic research, are still far from practical application in pediatrics.
- Published
- 2021
- Full Text
- View/download PDF
50. Turmeric (Curcuma longa L.) food supplements and hepatotoxicity: an integrated evaluation approach.
- Author
-
Menniti-Ippolito F, Ippoliti I, Pastorelli AA, Altieri I, Scalise F, De Santis B, Debegnach F, Brera C, Pacifici R, Pichini S, Pellegrini M, Rotolo MC, Graziano S, Palazzino G, Multari G, Gallo FR, Neri B, Giannetti L, Russo K, Fedrizzi G, Bonan S, Mazzanti G, Moro PA, Salvi E, Firenzuoli F, Valeri A, Moretti U, Traversa G, Silano M, Stacchini P, and Boniglia C
- Subjects
- Adult, Aged, Female, Humans, Italy, Male, Middle Aged, Chemical and Drug Induced Liver Injury etiology, Curcuma adverse effects, Dietary Supplements adverse effects, Plant Extracts adverse effects
- Abstract
Introduction: Turmeric is the common name for the rhizome of Curcuma longa L. In the recent years, food supplements containing turmeric have been marketed and widely used by an increasing number of consumers. Spontaneous reports of suspected adverse reactions to food supplements are collected within the Phytovigilance system., Methods: An ad hoc multidisciplinary group investigated the suspected cases of hepatotoxicity reported to the Italian Phytovigilance system associated with the assumption of turmeric food supplements with the methodology specific to pharmacovigilance as well as for the evaluation of the quality and safety of food supplements., Results: A cluster of 28 spontaneous reports of acute hepatitis, mostly with cholestasis, associated with turmeric products were sent to the Italian Phytovigilance system in the first six months of 2019. In all cases, except one, the causality assessment was at least possible. The suspected products were collected and analysed for the presence of drugs, heavy metals, aflatoxins, pesticides, synthetic dyes and pyrrolizidine alkaloids., Conclusion: On the basis of the results of all the activities performed by multidisciplinary group, regulatory intervention was taken. This study highlights the importance of developing an integrated evaluation approach for the evaluation of the adverse effects associated with the use of food supplements.
- Published
- 2020
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.