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Background: Exogenous gonadotropin-controlled ovarian stimulation is the critical step in animal reproductive management, such as pig, sheep, bovine and other species. It helps synchronize ovulation or stimulate multiple ovulations. However, a number of evidence indicated an unexpected decrease in pregnancy outcomes following ovarian stimulation. This study aimed to explore the underlying mechanism of the pregnancy defect and develop a practical rescue strategy. Results: Compared with those in the control group, gilts that underwent ovarian stimulation showed a decrease in pregnancy rate, farrowing rate, and total number of piglets born. Stimulated gilts also showed an increase in estradiol (E2) levels. The supraphysiological E2 level was correlated with the decrease in the number of piglets born. Furthermore, we found that high levels of E2 impair uterine receptivity, as shown by the overproliferation of endometrial epithelial cells. In vitro mechanistic studies demonstrated that high levels of E2 hyperactivate FGF-FGFR-ERK signaling cascade in the uterine endometrium, and in turn induces overproliferation of endometrial epithelial cells. Of note, N-acetyl-L-cysteine (NAC) supplementation effectively inhibits ERK hyperphosphorylation and ameliorates endometrial epithelial overproliferation. Importantly, in vivo experiments indicated that dietary NAC supplementation, compared with ovarian stimulation group, improves the uterine receptivity in gilts, and significantly increases the pregnancy rate and total number of piglets born. Conclusions: Ovarian stimulation-induced supraphysiological levels of E2 impairs uterine receptivity by hyperactivating FGF-FGFR-ERK signaling cascade, thereby reducing pregnancy rate and litter size. Supplementing NAC to a conventional diet for gilts ameliorates hyperactivated ERK signaling and improves uterine receptivity, thus rescuing adverse pregnancy outcomes following ovarian stimulation. [ABSTRACT FROM AUTHOR]

Introduction: Getah virus (GETV) is a multi-host virus found in pigs, horses, and blue foxes. Clinically, GETV can cause fever, diarrhea, and reproductive disorders in pigs, representing significant threats to pig breeding. At present, few studies have examined the pathogenicity of GETV in pigs of different ages. Methods: In the present study, a new strain, named GETV-QJ, was isolated from clinically ill pigs, and whole genome sequencing analysis was performed. Besides the pathogenicity of piglets and pregnant sows of this strain was further studied. Results: the results illustrated that the strain belonged to group III. The strain had 93.6%-96.3% homology with other subtypes, and its homology with the same subtype strain ranged 96.5%-99%. Further studies on the pathogenicity of the virus indicated that this strain caused severe diarrhea, fever, and intestinal and lung damage in 7-day-old piglets, resulting in their death. The piglet survival rate was 0%. In pregnant sows, this strain did not cause fever, death, or abortion, but it induced viremia, which affected the farrowing performance of sows and led to reduced piglet survival. Discussion: In this study, we isolated a highly virulent group III and comprehensively established a pathogenic model of GETV in piglets and sows, providing a reference and guidance for the prevention and control of this infection. [ABSTRACT FROM AUTHOR]

Animal boredom is a potentially prevalent, but underresearched animal welfare concern. To study the characteristics of boredom and its welfare consequences, we need to be sure that animals are actually bored and do not suffer from other negatively valenced states like apathy and depression. Animals' responses towards stimuli of different valence (positive, ambiguous, negative) have been suggested to help differentiating between these states. Apathetic animals are hypothesised to show a decreased interest in stimuli of all valences, whereas depressed animals are thought to be less interested in positive stimuli only, due to anhedonia, a key symptom of depression. In contrast, bored animals are hypothesised to show an increased interest in all types of stimuli, including negative ones. To ensure that the applied stimuli are indeed judged as positive, ambiguous or negative by the animals, we aimed to validate the valence of a range of stimuli in domesticated pigs, a species commonly kept under barren and monotonous conditions likely to induce boredom, as a basis for developing a task to distinguish between different negative states. Applying a within-subject design, 39 pigs (20 weaned piglets, 19 gilts) were individually exposed to twelve stimuli pre-classified as positive, ambiguous or negative in an approach-avoidance paradigm. The effects of stimulus, age (piglet, gilt) and their interaction on various approach and avoidance measures were analysed. Stimulus had a statistically significant effect on all measures and the observed pattern was according to pre-classification for many stimuli, but not all, resulting in a re-classification of the valence of five stimuli. The significant interaction between stimulus and age for some outcome measures indicates that age differences should be considered. Our study paves the ground for the selection of stimuli as well as outcome measures of future tasks aiming to differentiate between boredom, depression and apathy in pigs. [ABSTRACT FROM AUTHOR]

Erysipelas still causes large economic losses to pig industry. Maternal immunity is critical to prevent erysipelas in young animals, thus, intensive vaccination protocols or practices focused on the improvement of the maternally derived immunity could provide substantial benefits. The present study evaluates potential changes in antibodies levels in sows and their offspring using two types of tests (commercial ELISA, Ingenasa or rSpaA415 ELISA) when two different vaccination programs (before farrowing or after farrowing) against Erysipelothrix rhusiopathiae were applied to sows from Iberian (A) or conventional Large White-Landrace (B) pig farms. The results showed a statistical correlation between titers found in sows and their one-week old piglets in both tests. The overall mean of (log) antibody titers in farm B measured by the commercial ELISA test was significantly higher in pre-farrowing vaccinated sows compared to the post-farrowing vaccine protocol (p = 0.0278). Additionally, using the rSpaA415 ELISA test, the overall mean of (log) antibody titers was significantly higher in pre-farrowing sows (p = 0.0056) compared to sows following post-farrowing vaccine protocol (p = 0.0003) or non- vaccinated sows. None of the above-mentioned differences were found in farm A. The overall mean of (log) antibody titers in piglets from the pre-farrowing vaccination protocol was significantly higher than piglets from the post-farrowing vaccination protocol in farm A (p = 0.0059; rSpaA415 ELISA) and farm B (p = 0.0168 and p = 0.0098 for the commercial and rSpaA415 ELISA data, respectively). Additionally, higher proportion of piglets from pre-farrowing vaccinated sows remained seropositive during the post-weaning period (days 42 to 84) compared to piglets from non-vaccinated or post-farrowing vaccinated groups in both farms A and B. [ABSTRACT FROM AUTHOR]

Background: Progressive oxidative stress easily occurs as a result of a gradual increase in the intensity of maternal metabolism due to rapid foetal development and increased intensity of lactation. However, studies on the effects of processive oxidative stress on nutrient transport in the placenta have received little attention. The present study was conducted on sows at 85 days of gestation to study the effects of pterostilbene (PTE) on maternal oxidative stress status and placental nutrient transport. Results: PTE increased the antioxidant capacity and immunoglobulin content in mothers' blood and milk, reduced the level of inflammatory factors, and improved the nutrient content of milk. PTE also reduced sow backfat loss and the number of weak sons, and increased piglet weaning weight and total weaning litter weight. We subsequently found that PTE enhanced placental glucose and fatty acid transport and further affected glycolipid metabolism by increasing the expression of LAL, PYGM, and Gbe-1, which activated the PI3K phosphorylation pathway. Moreover, PTE addition altered the relative abundance of the Firmicutes, Proteobacteria, Parabacillus, and Bacteroidetes-like RF16 groups in sow faeces. PTE increased the levels of acetate, propionate, butyrate and isovalerate in the faeces. Conclusions: These findings reveal that the addition of PTE during pregnancy and lactation mitigates the effects of processive oxidative stress on offspring development by altering maternal microbial and placental nutrient transport capacity. [ABSTRACT FROM AUTHOR]

Background: Lawsonia intracellularis is the causative agent of Porcine Proliferative Enteropathy (PPE), one of the most prevalent pig enteric diseases worldwide, but with sparse information about early infections in suckling piglets in the epidemiology of PPE. With that aim, this study evaluates the prevalence of L. intracellularis in 3-week-old piglets by analysing ileal digesta content and mucosal scrapings from 383 pigs from 16 farms (aprox., 25 pigs/batch) by real-time qPCR and droplet digital PCR (ddPCR). Results: Forty-nine samples yielded a qPCR positive result. Eleven samples from eight farms were confirmed as positive with concentrations of L. intracellularis from 3.5 log10 to 4.5 log10 bacteria/g of sample. Another 16 samples, eight farms, were classified as low positive (2.07–2.38 log10 bacteria/g) and 22 provided an uncertain result. Finally, 334 samples tested negative for L. intracellularis. At batch level, half of the farms included in the study had at least one positive sample and in 10 farms (62.5%) there was at least one low positive sample. The ddPCR was run in 50 of the 383 samples based on their PCR output (including low positive, uncertain and negative samples). Correlation analyses revealed a strong association between qPCR and the ddPCR results (ρ = 0.75; p < 0.001). The ddPCR allowed us to detect and confirm a positive result in the 19 samples classified as uncertain by the qPCR and detect L. intracellularis in 8 of 15 negatives by qPCR. Conclusions: The results of the study demonstrate that a number of piglets are already infected with L. intracellularis during the suckling period evidencing early infection in certain animals, adding information of PPE epidemiology and opening new research topics such as sow-piglet transmission. Study results also evidence the usefulness of a combination of qPCR and ddPCR to improve qPCR sensitivity but assuring high specificity. [ABSTRACT FROM AUTHOR]

Background: A Tonsil-Oral-Scrubbing (TOSc) method was developed to sample the sow's oropharyngeal and tonsillar area without snaring and has shown comparable porcine reproductive and respiratory syndrome virus (PRRSV) RNA detection rates with tonsil scraping in infected sows. This study investigated the effect of specific TOSc collection factors on the PRRSV RT-rtPCR results (detection rates and Ct values). Those factors include whether the sow was snared or not snared at TOSc collection ("snared" vs. "not snared"); whether the sow was laying down or standing at collection ("laying down" vs. "standing"); and type of collectors used for TOSc collection ("TOSc prototype" vs. "Spiral-headed AI catheter (SHAC)"). Volume of fluid was compared between "snared" and "not snared" groups, and collection time was compared between "laying down" and "standing" groups as well. Results: The effect for each factor was assessed in three independent studies following the same design: TOSc was collected twice from each studied sow, once with the baseline level for a factor ("not snared", or "standing", or "TOSc prototype"), and another time followed by the other level of the paired factor ("snared", "laying down", or "SHAC", correspondingly). Results showed that "not snared" TOSc had numerically higher PRRSV RNA detection rate (60.7% vs. 52.5%, p = 0.11), significantly lower median Ct values (31.9 vs. 32.3, p < 0.01), and significantly higher volume of fluid than "snared" samples (1.8 mL vs. 1.2 mL, p < 0.01); "laying down" TOSc samples did not differ statistically (60.7% vs. 60.7%) in the PRRSV RNA detection rate, obtained numerically lower median Ct values (30.9 vs. 31.3, p = 0.19), but took 40% less collection time compared to "standing" TOSc samples; samples collected using the "TOSc prototype" had numerically higher PRRSV RNA detection rate (91.7% vs. 88.3%, p = 0.27) and significantly lower median Ct values (32.8 vs. 34.5, p < 0.01) than that from "SHAC". Conclusions: Under the conditions of this study best practices for TOSc collection aiming higher detection rate of PRRSV RNA while minimizing time for collection were suggested to be sampling TOSc without snaring, when sows are laying down, and using a prototype TOSc collector. [ABSTRACT FROM AUTHOR]

Simple Summary: This study reports the results of two studies aiming to determine, under commercial farm conditions, the effects of electronic sow feeders on the production and economic yields of lactating sows. The results indicate remarkable technical and economic outputs compared with traditional feeders due to the weaning of heavier piglets with a lower amount of feed per kg of weaned piglet. Adequate nutritional management in maternities is one of the most challenging aspects of swine production. This study reports the results of two studies aiming to determine, under commercial farm conditions, the effects of precision feeding (electronic sow feeders, ESFs) on the production and economic yields of lactating sows and possible nutritional and metabolic differences when compared to a control group fed with traditional feeders. The first trial showed that sows fed with ESFs weaned heavier piglets than sows fed with traditional feeders. Feed intake during the lactation period was similar in the sows of both groups; consequently, the amount of feed per kg of weaned piglet was lower in the sows fed with ESFs, which is a remarkable economic output. The second trial confirmed these findings and showed that, despite similar feed intakes, the sows fed with ESFs had lower bodyweight losses during the lactation period, but there were no major differences in milk composition or metabolic traits of sows and piglets. [ABSTRACT FROM AUTHOR]

Nowadays, video monitoring of farrowing and automatic video evaluation using Deep Learning have become increasingly important in farm animal science research and open up new possibilities for addressing specific research questions like the determination of husbandry relevant indicators. A robust detection performance of newborn piglets is essential for reliably monitoring the farrowing process and to access important information about the welfare status of the sow and piglets. Although object detection algorithms are increasingly being used in various scenarios in the field of livestock farming, their usability for detecting newborn piglets has so far been limited. Challenges such as frequent animal occlusions, high overlapping rates or strong heterogeneous animal postures increase the complexity and place new demands on the detection model. Typically, new data is manually annotated to improve model performance, but the annotation effort is expensive and time-consuming. To address this problem, we propose a Noisy Student approach to automatically generate annotation information and train an improved piglet detection model. By using a teacher-student model relationship we transform the image structure and generate pseudo-labels for the object classes piglet and tail. As a result, we improve the initial detection performance of the teacher model from 0.561, 0.838, 0.672 to 0.901, 0.944, 0.922 for the performance metrics Recall, Precision and F1-score, respectively. The results of this study can be used in two ways. Firstly, the results contribute directly to the improvement of piglet detection in the context of birth monitoring systems and the evaluation of the farrowing progress. Secondly, the approach presented can be transferred to other research questions and species, thereby reducing the problem of cost-intensive annotation processes and increase training efficiency. In addition, we provide a unique dataset for the detection and evaluation of newborn piglets and sow body parts to support researchers in the task of monitoring the farrowing process. [ABSTRACT FROM AUTHOR]

Serum from 88 pregnant sows and gilts was sampled 24 and 28 days after their first insemination or mating day. The oestrone sulphate (E1S) concentration in the samples was assessed with a commercially available radioimmunoassay kit modified for use with swine serum. The first aim was to test whether it was possible to predict litters of total number <10 piglets at term. The second aim was to compare the use of day 24 or day 28 samples, or of both, in this prediction. Day 24 E1S levels were positively correlated with litter size at term (R2 = 0.26; p < 0.001). E1S levels on day 28 were correlated with the levels on day 24 in the same animals but could not be used for prediction of large or small litters. The odds ratio for a small litter size was 0.16 (p < 0.01). This means that odds for a litter size <10 piglets decreased by 84% when E1S levels increased by 1.0 ng/ml. [ABSTRACT FROM AUTHOR]

Background: The administration of a gonadotropin releasing factor (GnRF) analog to pigs has proven to induce antibodies against endogenous GnRF. In gilts (young female pigs), the subsequent blocking of GnRF activity by specific antibodies results in a temporary suppression of ovarian activity and sexual maturation. One pre-clinical and two clinical studies were conducted to assess the ability of the GnRF analog to produce immunologically suppression of the ovarian function, preventing gilts from reaching puberty before harvest, at 27 weeks of age. Results: In the three studies, a significant reduction of size and weight of reproductive organs and gilts in oestrus was demonstrated in vaccinated gilts compared with intact gilts. A significant increase in anti-GnRF antibody levels in sera was observed after the 2nd dose, which lasted until the end of the study in each of the protocols used. Progesterone levels were significantly reduced from 6 to 8 weeks after 2nd vaccination in clinical studies 2 and 1 respectively, and from 6 weeks after 2nd vaccination in the pre-clinical study. Estradiol levels were below the limit of detection for clinical study 2 and significantly reduced in vaccinated gilts at the end of the pre-clinical study and the clinical study 1. Conclusions: Vaccination of gilts with a GnRF analog with two different protocols (1st dose from 10 to 14 weeks of age, and a 2nd dose 8 or 4 weeks later) was effective in reducing the development of puberty for at least 9 weeks post 2nd dose. These results confirm the flexibility of vaccination programs for veterinarians and producers which can be adapted to pig management practices in commercial farms. [ABSTRACT FROM AUTHOR]

The stress response of pig herds poses a significant challenge in the pig breeding industry, and investigating strategies to mitigate this stress is of paramount importance. The objective of this study was to investigate the impacts of supplemental feeding of Chinese herbal mixtures to perinatal sows on antioxidant capacity and gut microbiota of sows and their offspring piglets. A total of 60 healthy sows (Large white) at fourth parity were randomly assigned to five treatment groups. The control group received a basal diet, while the TRT1 group received a basal diet supplemented with 2kg/t Bazhen powder (BZP). The TRT2, TRT3, and TRT4 groups were fed a basal diet supplemented with 1kg/t, 2kg/t, and 3kg/t Qi-Zhu- Gui-Shao soothing liver and replenishing blood powder (QZGSP), respectively. The trial lasted for 5weeks, starting from day 100 of gestation until day 21 of delivery. The results demonstrated that the inclusion of 2kg/t and 3kg/t QZGSP significantly enhanced the antioxidant capacity of sows and their offspring piglets to different degrees, thereby effectively alleviating oxidative stress. Analysis of gut microbiota revealed that QZGSP influenced the composition of gut microbiota in both sows and their offspring piglets. Specifically, at the genus level, the abundance of Christensenellaceae&lowbar;R-7&lowbar;group in the gut microbiota of sows in the TRT4 group was significantly lower than that in the TRT1 group (p < 0.05), while the relative abundance of Lactobacillus in the gut microbiota of sows in the TRT4 group was significantly higher than that in the CON group (p < 0.05). Furthermore, at the genus level, compared to those in the TRT1 group, piglets from the TRT4 group exhibited a significant decrease in relative abundance of Escherichia-Shigella, Parabacteroides, and Methanobrevivacter (p < 0.05), but a significant increase in Phascolarctobacterium (p < 0.05). Spearman correlation analysis indicated a positive correlation between relative abundance of Christensenellaceae&lowbar;R-7&lowbar;group and serum contents of T-AOC and CAT (p < 0.05), as well as a negative correlation with serum concentration MDA (p < 0.05). Additionally, there was a positive correlation between relative abundance Lactobacillus and serum levels SOD (p < 0.01) and GSH-Px (p < 0.05). Therefore, supplementation of 3kg/t QZGSP in the periparturient sow diet significantly augmented antioxidant capacity in both sows and offspring piglets, while concurrently modulating the composition and structure of their intestinal microflora. The findings from this study demonstrate that QZGSP represents a beneficial feed additive for perinatal sows. [ABSTRACT FROM AUTHOR]

Simple Summary: Sow parturition, the process of giving birth to piglets, is a highly stressful process, with outcomes affecting farm economics as well as sow and piglet welfare. Protocols that assist with the identification of abnormal parturition are required for optimal production outcomes. The eye is an easily accessible and anatomically consistent body region, often used to aid medical diagnoses. A novel three-level ocular scoring system was devised and used to investigate the relationship between changes in ocular appearance and farrowing kinetics. It was determined that ocular appearance scoring can be used at farrowing to aid identification of sows experiencing an abnormal parturition, as changes are associated with increased straining and extended farrowing durations. This is a first step in developing a scale that may aid in predicting an extended parturition (>300 min), identifying sows that require assistance and so aiding staff management, sow welfare, and piglet survival. Protocols that enable prompt identification of sows in need of assistance during farrowing are important for optimal production outcomes. Change in the colour and appearance of the sclera of the eye can indicate increased stress. This warrants investigation into its use for the identification of sows in need of assistance at the time of parturition. To this end, a three-level ocular scoring system for the pig was devised and subsequently used in a preliminary investigation into the relationship between farrowing kinetics and visual changes in the sclera at farrowing. Data were collected and analysed from twenty randomly selected Large White × Landrace crossbred sows during farrowing. It was determined that sows with a severe ocular score were more likely to experience a prolonged farrowing duration (p = 0.013) and incur increased parturient straining of either total straining time (p = 0.011) or straining per piglet (p = 0.025). There was a significant association between ocular score and litter size (p = 0.043). Ocular score was not associated with sow parity (p = 0.728) and inter-piglet interval (p = 0.075). The proposed three-level scoring system successfully identified sows experiencing an abnormal parturition as defined by a prolonged farrowing duration and increased straining time. Findings from this study suggest the potential application of this simple ocular scoring tool for identifying sows experiencing an abnormal farrowing via in-person application or integration into remote monitoring systems in the future. [ABSTRACT FROM AUTHOR]

The Maternity Ring was developed as a free farrowing alternative to crates that preserved space whilst providing the sow with unrestricted movement. This experiment aimed to apply the Five Domains model to assess sow welfare in the Maternity Ring in comparison with the farrowing crate. Eighty-eight sows were housed in a farrowing crate (FC) and 83 in a Maternity Ring (MR), and measures collected focussed on nutrition, environment, health, behaviour, and mental state outcomes. MR sows consumed less feed than FC sows (total feed intake: 93.8  ±  3.06  kg vs. 111.2  ±  3.13  kg; p  <  0.001) but had reduced P2 backfat loss during lactation (0.0  ±  0.11 vs. 1.2  ±  0.11, p  <  0.001). Fewer frustrated and pain-related behaviours during farrowing were observed in MR sows (bar biting: FC 3.3  ±  2.12 vs. MR 0.5  ±  0.29 events, p  =  0.038, and back leg forward: FC 227  ±  50.7 vs. MR 127  ±  26.4 events, p  =  0.019), and a decreased proportion of MR sows had facial injuries after farrowing (10% CI [5, 20] vs. 67% CI [47, 95], p  <  0.001). More FC sows had udder damage at weaning (70% CI [48, 97] vs. 10% CI [6, 24], p  <  0.001), and their piglets were medicated more frequently when compared to those in MR (51% CI [40, 61] vs. 30% [21, 41], p  =  0.008). MR sows tended to have a higher reaction score to piglet processing (MR 2.0  ±  0.38 vs. FC 1.2  ±  0.27, p  =  0.094) and had more contact with piglets once the procedure was complete than FC sows (13.5  ±  2.55 vs. 6.9  ±  1.26 events, respectively, p  =  0.016). Whilst there was no difference in anticipation of a feeding event (p  >  0.05), MR sows displayed a reduced startle response to an aversive noise stimulus at day 18 (FC 2.8  ±  0.35, MR 0.7  ±  0.16, p  <  0.001). Using the Five Domains framework, sows housed in the MR during farrowing and lactation have improved welfare than those in FC and can be thought of as being in a positive affective state. [ABSTRACT FROM AUTHOR]

The flow cytometry method is used in many fields, not only scientific, but also clinical. In science, flow cytometry is used in immunology, molecular biology, microbiology or plant biology. In medicine, has its use, in the diagnosis of tumours, in reproductive and prenatal diagnosis, in transplants or in haematology. In our experimental work, we used this method to analyse colostrum and milk of sows. The aim of this study was to determine the number of somatic cells, the number of leukocytes and the number of T-lymphocytes. Colostrum samples were collected within 24 hours of delivery and then milk samples were collected at weekly intervals during 3 weeks. Statistical analysis revealed significant differences in the number of leukocytes (CD45+) in colostrum and milk. The most significant difference was noted between colostrum and milk in weeks 2 and 3 of the experiment (P < 0.0001), with the highest values found in colostrum. On the other hand, we found that the levels of helper T-cells (CD4+) and cytotoxic T-cells (CD8+) gradually increased over time (P > 0.05), with the highest values observed in the third week after farrowing. We can conclude that flow cytometry can be successfully used for the examination of somatic cells in sows' milk. [ABSTRACT FROM AUTHOR]

Abstract Background Progressive oxidative stress easily occurs as a result of a gradual increase in the intensity of maternal metabolism due to rapid foetal development and increased intensity of lactation. However, studies on the effects of processive oxidative stress on nutrient transport in the placenta have received little attention. The present study was conducted on sows at 85 days of gestation to study the effects of pterostilbene (PTE) on maternal oxidative stress status and placental nutrient transport. Results PTE increased the antioxidant capacity and immunoglobulin content in mothers’ blood and milk, reduced the level of inflammatory factors, and improved the nutrient content of milk. PTE also reduced sow backfat loss and the number of weak sons, and increased piglet weaning weight and total weaning litter weight. We subsequently found that PTE enhanced placental glucose and fatty acid transport and further affected glycolipid metabolism by increasing the expression of LAL, PYGM, and Gbe-1, which activated the PI3K phosphorylation pathway. Moreover, PTE addition altered the relative abundance of the Firmicutes, Proteobacteria, Parabacillus, and Bacteroidetes-like RF16 groups in sow faeces. PTE increased the levels of acetate, propionate, butyrate and isovalerate in the faeces. Conclusions These findings reveal that the addition of PTE during pregnancy and lactation mitigates the effects of processive oxidative stress on offspring development by altering maternal microbial and placental nutrient transport capacity. Graphical Abstract

This study aimed to investigate genetic parameters for sow pelvic organ prolapse in purebred and crossbred herds. Pelvic organ prolapse was recorded as normal or prolapsed on the individual sow level across 32 purebred and 8 crossbred farms. In total, 75,162 purebred Landrace sows from a single maternal line were recorded between 2018 and 2023, while 18,988 commercial two-way crossbred (Landrace x Large White) sows were available between 2020 and 2023. There were 5,122,005 animals included in the pedigree. The prolapse in purebreds and crossbreds was considered two different traits in the model. Pedigrees of the crossbred sows were determined based on genotypes through parentage assignment. The average incidence rates were 1.81% and 3.93% for purebreds and crossbreds, respectively. The bivariate model incorporated fixed effects of parity group and region with random effects of contemporary group (farm and mating year and month at the first parity), additive genetic, and residual. Genetic parameter estimates were obtained using BLUPF90+ with the AIREML option. The estimated additive variance was larger in crossbreds than in purebreds. Estimates of heritability in the observed scale were 0.09 (0.006) for purebreds and 0.11 (0.014) for crossbreds, with a genetic correlation of 0.83 using a linear model. Results suggested that including data from crossbreds with higher incidence rate is beneficial and selection to reduce the prolapse incidence in purebred sow herds would also benefit commercial crossbred sow herds. [ABSTRACT FROM AUTHOR]

Introduction: Claw lesions significantly contribute to lameness, greatly affecting sow welfare. This study investigated different factors that would impact the severity of claw lesions in the sows of Brazilian commercial herds. Methods: A total of 129 herds (n = 12,364 sows) were included in the study. Herds were in the Midwest, Southeast, or South regions of Brazil. Inventory sizes were stratified into 250-810 sows, 811-1,300 sows, 1,301-3,000 sows, and 3,001-10,000 sows. Herds belonged to Cooperative (Coop), Integrator, or Independent structures. The herd management was conducted either maintaining breeds from stock on-site (internal), or through purchase of commercially available genetics (external). Herds adopted either individual crates or group housing during gestation. Within each farm, one randomly selected group of sows was scored by the same evaluator (two independent experts evaluated a total of 129 herds) from 0 (none) to 3 (severe) for heel overgrowth and erosion (HOE), heel-sole crack (HSC), separation along the white line (WL), horizontal (CHW) and vertical (CVW) wall cracks, and overgrown toes (T), or dewclaws (DC) in the hind legs after parturition. The study assessed differences and similarities between herds using Principal Component Analysis (PCA) and Hierarchical Agglomerative Clustering (HAC) analysis. The effects of factors (i.e., production structure, management, housing during gestation, and region) were assessed using the partial least squares method (PLS). Results and discussion: Heel overgrowth and erosion had the highest prevalence, followed by WL and CHW, while the lowest scores were observed for T DC, and CVW. Herds were grouped in three clusters (i.e., Cl, C2, and C3). Heel overgrowth and erosion, HSC, WL, CHW, CVW, and T were decreased by 17, 25, 11, 25, 21, and 17%, respectively, in C3 compared to CI and 2 combined. Independent structure increased the L-lndex in all three clusters. Furthermore, individual housing increased the L-lndex regardless of the cluster. The results suggest that shifting toward larger, more technologically advanced herds could potentially benefit claw health. Additionally, adopting group gestation housing appears to mitigate the adverse effects on claw health, although further validation is necessary, as Brazil has only recently transitioned from individual housing practices. [ABSTRACT FROM AUTHOR]

Large White and Meishan sows differ in maternal ability and early piglet growth. We investigated the relationships between 100 maternal traits, grouped into 11 blocks according to the biological function they describe and litter growth over three successive periods after birth (D0–D1, D1–D3 and D3–D7; D0 starting at the onset of farrowing), as a measure of sow investment in early piglet production. Within- and between-breed variation was exploited to cover a maximum of the variability existing in pig maternal populations. The objective was to quantify the contribution of maternal traits, including functional traits and behavioural traits, to early litter growth. Multivariate analyses were used to depict correlations among traits. A partial least square multiblock analysis allowed quantifying the effect of maternal traits on early growth traits. Partial triadic analyses highlighted how sow behaviour changed with days, and whether it resulted in changes in litter growth. Several behavioural traits (standing activity, reactivity to different stimuli, postural activity) and functional traits (body reserves, udder quality) at farrowing contributed substantially to litter growth from D0 to D7. Sow aggression towards piglets and time spent standing at D0 were unfavourably correlated to D1–D3 litter growth. Time spent lying with udder exposed at D0 was favourably correlated to D1–D3 litter growth. The farrowing duration was negatively correlated to D0–D1 and D1–D3 litter growth. Furthermore, D3–D7 litter growth was positively correlated to feed intake in the same period. Several behavioural traits and some functional traits influence early litter growth. The contribution of sow behaviour was greater in the critical period around farrowing than in later days. [ABSTRACT FROM AUTHOR]

Introduction: Sow mortality in the U.S. swine industry has increased in recent years, for which pelvic organ prolapse (POP) is a major contributor, accounting for 21% of all sow mortality. Dysbiosis of microbial communities has been associated with disease and reproductive dysfunction in several species, and previous studies have shown changes in vaginal microbiota in sows with increased risk for POP during late gestation. However, there is insufficient knowledge surrounding the potential relationship between fecal microbiota and POP in sows. Therefore, the study objective was to identify differences in sow fecal microbiota and determine if fecal and vaginal microbial communities are correlated in relation to POP risk. Methods: Sows were evaluated for POP risk using an established perineal scoring system, with a perineal score (PS) of 1 (PS1) presuming little to no risk of POP to a PS of 3 (PS3) presuming high risk of POP. In the current study, 2,864 sows were scored during gestation week 15, and 1.0%, 2.7%, and 23.4% of PS1, PS2, and PS3 sows, respectively, subsequently experienced POP. Fecal swabs (n = 215) were collected between gestation days 108-115, DNA was extracted, and 16S rRNA gene amplicon sequencing libraries were analyzed using mothur, phyloseq and SAS in reference to PS and POP outcome. Additionally, co-occurrence networks were constructed using CoNet to compare fecal and vaginal microbiota from the same cohort of sows and identify correlations between different taxa. Results: Differences in fecal community composition (PERMANOVA; P < 0.05), structure (alpha diversity measurements; P < 0.05), and 13 individual operational taxonomic units (OTUs) were revealed between PS1 and PS3 assigned sows. No differences in fecal microbiota were detected as a result of POP outcome. However, the abundances of several taxa were correlated across sample collection sites, suggesting the fecal and vaginal microbial communities may be related to one another. Discussion: Collectively, fewer differences in the fecal microbiota exist in sows with differing risk for POP compared to the vaginal microbiota, suggesting the vaginal microbiome may be more relevant in relation to POP outcome, although correlations between fecal and vaginal communities may provide insight for strategies to combat POP. [ABSTRACT FROM AUTHOR]

Background and Aims: The effects of second litter syndrome (SLS) on subsequent reproductive performance remain poorly understood. This study examined the impact of SLS on reproductive parameters such as piglets born alive (PBA), accumulative number of PBA (APBA), farrowing interval (FI), and risk of decreased PBA (DPBA) up to parity 5. Materials and Methods: Data on 5,464 litters were recorded from 1,507 sow cards collected on five swine farms in northern Vietnam. A linear mixed-effect model was used to analyze the effect of SLS on the PBA, APBA, and FI. A generalized linear mixed model was used to analyze the effect of DPBA in parity n on the risk of DPBA in parity n + 1. Results: About 47.8% of the sows contracted SLS (720/1507). Only APBA1-2 was significantly decreased by SLS. The APBA3-5 in SLS sows was comparable to that in non-SLS sows (41.8 vs. 41.9). Non-DPBA2 upped the risk for DPBA3 by 3.6-fold (95% confidence interval [CI]: 2.8-4.6). Moreover, non-DPBA3 increased the risk of DPBA4 (odds ratio [OR] = 2.7, 95% CI = 2.1-3.7), and non-DPBA4 increased the risk of DPBA5 (OR = 3.2, 95% CI = 2.3-4.7). The risks of developing DPBA4 and DPBA5 remained unchanged following SLS (p > 0.05). About 98.4% of sows underwent PBA fluctuations during their first five parities. Conclusion: SLS does not appear to detrimentally affect PBA, APBA, and FI in subsequent parities. Therefore, SLS sows do not necessarily have future low reproductive performance or be culled. Future investigations should explore the mechanism of alternate decrease/increase patterns in PBA. [ABSTRACT FROM AUTHOR]

Objective: Three experiments were conducted to evaluate the effects of Saccharomyces yeast postbiotics (SYP) in feeds for sows on the growth of offspring (Exp. 1), for nursery pigs on their growth (Exp. 2), and for nursery and finishing pigs on their growth (Exp. 3). Methods: Exp. 1 had 80 sows at breeding assigned to 4 groups with SYP at 0, 0.050, 0.175, and 0.500 g/kg. Offspring were fed a common diet for 126 d. Exp. 2 had 144 barrows at 8 kg body weight (BW) allotted to CON (no SYP); YPC (SYP at 0.175 g/kg; d 0 to 42); and YPD (SYP at 1.25, 0.75, and 0 g/kg; d 0 to 7, d 8 to 21, and d 22 to 42, respectively) with 8 pens/treatment (6 pigs/pen). Exp. 3 had 96 barrows at 8 kg BW allotted to CON (no SYP); YPN (SYP at 0.175 g/kg; d 0 to 42); YPF (SYP at 0.100 g/kg; d 43 to 119); and YPA (SYP at 0.175 and 0.100 g/kg; d 0 to 42 and d 43 to 119, respectively) with 8 pens/treatment (3 pigs/pen). Results: In Exp. 1, increasing SYP increased (p<0.05, quadratic) the sow body score (maximum at 0.30% SYP), reduced (p<0.05, quadratic) the days-wean-to-estrus (minimum at 0.27% SYP), and increased (p<0.05) offspring BW at weaning and their average daily gain (ADG) and feed efficiency (G:F) at d 126. In Exp. 2, ADG, average daily feed intake (ADFI), and G:F of YPC were the greatest (p<0.05). The ADG and ADFI of YPD were greater (p<0.05) than CON. Fecal score of YPC and YPD was smaller (p<0.05) than CON. In Exp. 3, YPA had the greatest (p<0.05) ADG and YPN and YPF had greater (p<0.05) ADG than CON. Conclusion: SYP enhanced sow performance, offspring growth, growth of nursery and growing pigs with the greater efficacy at 0.27 to 0.32 g/kg feed. [ABSTRACT FROM AUTHOR]

Objective: Litter size and piglet loss at birth significantly impact piglet production and are closely associated with sow parity. Understanding how these traits vary across different parities is crucial for effective herd management. This study investigates the patterns of the number of born alive piglets (NBA), number of piglet losses (NPL), and the proportion of piglet losses (PPL) at birth in Landrace sows under tropical conditions. Additionally, it aims to identify the most suitable model for describing these patterns. Methods: A dataset comprising 2,322 consecutive reproductive records from 258 Landrace sows, spanning parities from 1 to 9, was analyzed. Modeling approaches including 2nd and 3rd degree polynomial models, the Wood gamma function, and a longitudinal model were applied at the individual level to predict NBA, NPL, and PPL. The choice of the best-fitting model was determined based on the lowest mean and standard deviation of the difference between predicted and actual values, Akaike information criterion (AIC), and Bayesian information criterion (BIC). Results: Sow parity significantly influenced NBA, NPL, and PPL (p<0.0001). NBA increased until the 4th parity and then declined. In contrast, NPL and PPL decreased until the 2nd parity and then steadily increased until the 8th parity. The 2nd and 3rd degree polynomials, and longitudinal models showed no significant differences in predicting NBA, NPL, and PPL (p>0.05). The 3rd degree polynomial model had the lowest prediction standard deviation and yielded the smallest AIC and BIC. Conclusion: The 3rd degree polynomial model offers the most suitable description of NBA, NPL, and PPL patterns. It holds promise for applications in genetic evaluations to enhance litter size and reduce piglet loss at birth in sows. These findings highlight the importance of accounting for sow parity effects in swine breeding programs, particularly in tropical conditions, to optimize piglet production and sow performance. [ABSTRACT FROM AUTHOR]

This study aimed to determine the effects of different doses of Acremonium terricola culture (ATC) on lactation performance, immune function, antioxidant capacity, and intestinal flora of sows. Forty-five Landrace sows (3–6 parity) were randomly assigned to the following three treatments from 85 days of gestation to 21 days after farrowing: a control diet (CON, basal diet), a low-dose Acremonium terricola culture diet (0.2% ATC, basal diet + 0.2% ATC), and a high-dose Acremonium terricola culture diet (0.4% ATC, basal diet + 0.4% ATC). Compared with the CON group, the supplementation of 0.2% ATC increased the average daily milk yield of sows by 4.98%, increased milk fat, total solids, and freezing point depression on day 1 postpartum (p < 0.05), increased serum concentration of Triiodothyronine, Thyroxin, and Estradiol on day 21 postpartum (p < 0.05). Compared with the CON group, the supplementation of 0.4% ATC increased the average daily milk yield of sows by 9.38% (p < 0.05). Furthermore, the supplementation of 0.2% ATC increased serum concentration of IgG, IgM, and IFN-γ, CD4 on day 1 postpartum (p < 0.05) and increased serum concentration of immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM), complement 3 (C3), cluster of differentiation 4 (CD4), cluster of differentiation 8 (CD8), interferon-γ (IFN-γ) on day 21 postpartum (p < 0.05), while the supplementation of 0.4% ATC reduced serum concentration of IL-2 on day 21 postpartum (p < 0.05). Moreover, the supplementation of 0.4% ATC significantly increased serum concentration of catalase (CAT) (p < 0.05). Additionally, the supplementation of ATC affected the relative abundance of the intestinal flora at different taxonomic levels in sows and increased the abundance of beneficial bacteria such as in the norank_f__Eubacterium_coprostanoligenes group, Eubacterium_coprostanoligenes group, and Lachnospiraceae_XPB1014 group of sows, while reducing the abundance of harmful bacteria such as Phascolarctobacterium and Clostridium_sensu_stricto_1. These data revealed that the supplementation of ATC during late gestation and lactation can improve lactation performance, immune function, antioxidant capacity, and the gut microbiota. Compared with supplementation of 0.4% ATC, 0.2% ATC enhances the levels of thyroid-related hormones, specific antibodies, and cytokines in serum, promotes the diversity of beneficial gut microbiota, beneficial bacteria in the intestine, reduces the population of harmful bacteria, and thereby bolsters the immunity of sows. Hence, 0.2% ATC is deemed a more optimal concentration. [ABSTRACT FROM AUTHOR]

The production management of lactating sows is a crucial aspect of pig farm operations, as their health directly impacts the farm's production efficiency. The feeding behavior of lactating sows can reflect their health and welfare status, and monitoring this behavior is essential for precise feeding and management. To address the issues of time-consuming and labor-intensive manual inspection of lactating sows' feeding behavior and the reliance on breeders' experience, we propose a method based on the improved YOLO (You Only Look Once) v5s algorithm and image segmentation for detecting the feeding behavior of lactating sows. Based on the YOLOv5s algorithm, the SE (Squeeze-and-Excitation) attention module was added to enhance the algorithm's performance and reduce the probability of incorrect detection. Additionally, the loss function was replaced by WIoU (Weighted Intersection over Union) to accelerate the model's convergence speed and improve detection accuracy. The improved YOLOv5s-C3SE-WIoU model is designed to recognize pre-feeding postures and feed trough conditions by detecting images of lactating sows. Compared to the original YOLOv5s, the improved model achieves an 8.9% increase in mAP@0.5 and a 4.7% increase in mAP@0.5 to 0.95. This improvement satisfies the requirements for excellent detection performance, making it suitable for deployment in large-scale pig farms. From the model detection results, the trough remnant image within the detection rectangle was extracted. This image was further processed using image processing techniques to achieve trough remnant image segmentation and infer the remnant amount. Based on the detection model and residue inference method, video data of lactating sows' feeding behavior were processed to derive the relationship between feeding behavior, standing time, and residue amount. Using a standing duration of 2 s and a leftover-feed proportion threshold of 2% achieves the highest accuracy, enabling the identification of abnormal feeding behavior. We analyzed the pre-feeding postures and residual feed amounts of abnormal and normal groups of lactating sows. Our findings indicated that standing time was significantly lower and residual feed amount was higher in the abnormal groups compared to the normal groups. By combining standing time and residual feed amount information, accurate detection of the feeding status of lactating sows can be realized. This approach facilitates the accurate detection of abnormal feeding behaviors of lactating sows in large-scale pig farm environments. [ABSTRACT FROM AUTHOR]

Background: Teat number is a vital reproductive trait in sows, crucial for providing immunity and nutrition to piglets during lactation. However, "missing heritability" in Single Nucleotide Polymorphism (SNP)-based Genome-Wide Association Studies (GWAS) has led to an increasing focus on structural variations in the genetic analysis of complex biological traits. Results: In this study, we generated a comprehensive CNV map in a population of French Yorkshire pigs (n = 644) and identified 429 CNVRs. Notably, 44% (189 CNVRs) of these were detected for the first time. Subsequently, we conducted GWAS for teat number in the French Yorkshire pig population using both 80K chip and its imputed data, as well as a GWAS analysis based on CNV regions (CNVR). Interestingly, 80K chip GWAS identified two SNPs located on Sus scrofa chromosome 5 (SSC5) that were simultaneously associated with Total Teat Number (TTN), Left Teat Number (LTN), and Right Teat Number (RTN). The leading SNP (WU_10.2_5_76130558) explained 3.33%, 2.69%, and 2.67% of the phenotypic variance for TTN, LTN, and RTN, respectively. Moreover, through imputed GWAS, we successfully identified 30 genetic variants associated with TTN located within the 73.22 -73.30 Mb region on SSC5. The two SNPs identified in the 80K chip GWAS were also located in this region. In addition, CNVR-based GWAS revealed three significant CNVRs associated with TTN. Finally, through gene annotation, we pinpointed two candidate genes, TRIM66 and PRICKLE1, which are related to diverse processes such as breast cancer and abnormal vertebral development. Conclusions: Our research provides an in-depth analysis of the complex genetic structure underlying teat number, contributing to the genetic enhancement of sows with improved reproductive performance and, ultimately, bolstering the economic benefits of swine production enterprises. [ABSTRACT FROM AUTHOR]

Lameness affects animal mobility, causing pain and discomfort. Lameness in early stages often goes undetected due to a lack of observation, precision, and reliability. Automated and non-invasive systems offer precision and detection ease and may improve animal welfare. This study was conducted to create a repository of images and videos of sows with different locomotion scores. Our goal is to develop a computer vision model for automatically identifying specific points on the sow's body. The automatic identification and ability to track specific body areas, will allow us to conduct kinematic studies with the aim of facilitating the detection of lameness using deep learning. The video database was collected on a pig farm with a scenario built to allow filming of sows in locomotion with different lameness scores. Two stereo cameras were used to record 2D videos images. Thirteen locomotion experts assessed the videos using the Locomotion Score System developed by Zinpro Corporation. From this annotated repository, computational models were trained and tested using the open-source deep learning-based animal pose tracking framework SLEAP (Social LEAP Estimates Animal Poses). The top-performing models were constructed using the LEAP architecture to accurately track 6 (lateral view) and 10 (dorsal view) skeleton keypoints. The architecture achieved average precisions values of 0.90 and 0.72, average distances of 6.83 and 11.37 in pixel, and similarities of 0.94 and 0.86 for the lateral and dorsal views, respectively. These computational models are proposed as a Precision Livestock Farming tool and method for identifying and estimating postures in pigs automatically and objectively. The 2D video image repository with different pig locomotion scores can be used as a tool for teaching and research. Based on our skeleton keypoint classification results, an automatic system could be developed. This could contribute to the objective assessment of locomotion scores in sows, improving their welfare. [ABSTRACT FROM AUTHOR]

Simple Summary: Plant extracts (PEs) are a complex mixture of compounds that have been reported to possess multiple biological functions, including antioxidant, immunomodulatory, anti-inflammatory, and anti-microbial activities. PEs have become a viable alternative to antibiotics in animal diets, including sows. The present study showed that dietary Lonicera flos and Sucutellaria baicalensis mixed extract (LSE) supplementation improved the reproductive performance, immune status, reproductive hormone levels, and colostrum immunoglobulin contents in sows. This study can provide a reference for the rational utilization and application of honeysuckle and Scutellaria scutellaria in the sow diet. The present study aimed to determine the effects of dietary Lonicera flos and Sucutellaria baicalensis mixed extract (LSE) supplementation during the late-pregnancy period on the reproductive performance, umbilical cord blood hematological parameters, umbilical cord serum biochemical parameters, immune indices, hormone levels, colostrum ingredients, and immunoglobulin contents of sows. A total of 40 hybrid pregnant sows were randomly assigned to the control group (CON; sows fed a basal diet) and LSE group (LSE; sows fed a basal diet supplemented with 500 g/t PE). The results indicated that dietary LSE supplementation significantly increased (p < 0.05) the number of alive and healthy piglets and the litter weight at birth, and significantly increased (p < 0.05) the platelet counts in umbilical cord blood. Dietary LSE supplementation significantly increased (p < 0.05) the levels of prolactin (PRL) and growth hormone (GH), and the content of interleukin 2 (IL-2) in umbilical cord serum. Moreover, immunoglobulin A (IgA) and immunoglobulin M (IgM) in the colostrum were increased with PE supplementation (p < 0.05). In conclusion, dietary LSE supplementation in late-pregnancy sows could improve reproductive performance and colostrum quality, and could also regulate the levels of reproductive hormone in umbilical cord serum. [ABSTRACT FROM AUTHOR]

International interest in loose-housed farrowing is growing and there are ongoing discussions within the European Union (EU) on new legal requirements. However, there is a lack of empirical data on loose-housed farrowing pen sizes and sow dimensions in commercial production. The aim of this study was to map and describe sow size and loose-housing farrowing pen size on commercial piglet-producing farms in Sweden. The study included 146 sows and 51 pen types on 35 medium sized to large Swedish piglet-producing farms (ranging from 106 to 1300 sows in production). Sow length ranged from 129 to 238 cm (mean ± SD 191.3 ± 19.3 cm) and sow height from 74 to 133 cm (86.7 ± 7.7 cm). Floor space occupied by the sow when lying down (length x height) ranged from 1.0 to 3.2 m2 (1.7 ± 0.3 m2). Pen length ranged from 259 to 415 cm (315.1 ± 24.3 cm), pen width from 188 to 245 cm (207.0 ± 10.7 cm), total pen area from 5.7 to 8.9 m2 (6.5 ± 0.5 m2), piglet corner area from 0.5 to 1.8 m2 (1.1 ± 0.4 m2) and area available for the sow (total area - piglet corner area) from 3.9 to 6.4 m2 (5.4 ± 0.6 m2). These results show that there is substantial variation in sow, pen and piglet corner size on commercial piglet-producing farms in Sweden. This poses a risk of mismatches between sow and pen size (pens too short in relation to sow dimensions), especially for older sows. These findings are of practical significance for animal welfare and production and emphasise the importance of designing loose-housed pens adapted to future sow, litter and piglet size. [ABSTRACT FROM AUTHOR]

Background: Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is a significant swine pathogen, yet the immune response components contributing to protection remain incompletely understood. Broadly reactive neutralizing antibodies (bNAs) may play a crucial role in preventing reinfections by heterologous viruses, although their occurrence is considered low under both field and experimental conditions. This study aimed to assess the frequency of sows exhibiting bNAs against PRRSV under field conditions and to analyze the epidemiological factors influencing the occurrence of these elite neutralizers. Blood samples were collected from breeding sows across eleven unrelated pig farms, with samples categorized by parity. Serum obtained was utilized in virus neutralization assays (VNs) against six PRRSV field isolates and two MLV strains. Results: Approximately 7% of the sows exhibited neutralization activity against all viruses in the panel, with a geometric mean of the titer (GMT) of NAs at or exceeding 4 log2. Exclusion of the PRRSV-2 isolate from the panel increased the proportion of elite neutralizers to around 15%. Farm-specific analysis revealed significant variations in both GMT of NAs and proportion of elite neutralizers. PRRSV unstable farms and those with a PRRS outbreak in the last 12 months displayed higher GMT of NAs compared to stable farms without recent outbreaks. The GMT of NAs showed a gradual, albeit moderate, increase with the parity of the sows. Parity's impact on bNA response was consistently observed in stable farms but not necessarily in unstable farms or those with recent outbreaks. Finally, the results indicated that vaccinated animals had higher NA titers against the vaccine virus used in the farm than against field viruses. Conclusion: bNAs against heterologous isolates induced by PRRSV infection under field conditions are generally low, often falling below titers necessary for protection against reproductive failure. However, a subset of sows (approximately 15%) can be considered elite neutralizers, efficiently recognizing various PRRSV strains. Repeated exposures to PRRSV play a crucial role in eliciting these bNAs, with a higher frequency observed in unstable farms and those with recent outbreaks. In stable farms, parity only marginally influences bNA titers, highlighting its limited role compared to the impact of PRRSV exposure history. [ABSTRACT FROM AUTHOR]

Background: Most sows will experience negative energy balance during lactation resulting in impaired follicular development. This study aimed to treat 28-day lactating sows with altrenogest (ALT) to suppress follicle enlargement during lactation, and to assess the estrus and reproductive performance post-weaning. Methods: In this study, we conducted two trials. In trial 1, we monitored the follicular development of lactating sows including 10 primiparous sows and 10 multiparous sows during the whole lactation to confirm the ALT administration time. In trial 2, a total of 42 primiparous and 111 multiparous sows were allocated to three treatments: Ctrl (control group, n = 51): no treatment; TAI (timed artificial insemination group, n = 51): sows were injected with equine chorionic gonadotropin (eCG) after weaning 24 h and gonadotropin-releasing hormone (GnRH) when they expressed estrus; and AT-TAI (ALT treatment-timed artificial insemination group, n = 51): base on the process of TAI group, the sows were fed with 20 mg ALT per day before weaning 10 days. All sows were artificially inseminated twice at 12 h and 36 h after estrus. The follicle size changes and serum hormone levels were explored in this process. Results: Although the follicle size of multiparous sows was larger than primiparous sows during the whole lactation (P < 0.05), similar change trends of follicle size were observed in primiparous and multiparous sows. Meanwhile, the FSH, LH and E2 levels of multiparous sows were higher than primiparous sows. The ALT treatment significantly inhibits the increase in follicle size (P < 0.05) and reduces the serum levels of FSH, LH and E2 (P > 0.05). Additionally, ALT treatment increases estrus concentration and the preovulatory follicle size (P < 0.05), meanwhile, it delays the weaning-to-estrus interval (WEI, P < 0.001). However, the estrus rate, pregnancy rate, total pigs born and born alive did not differ between treatments (P > 0.05). Conclusions: There were significant differences in the size of follicles in the lactation between primiparous and multiparous sows. ALT treatment during the last ten days of lactation concentrated estrus expression leading to higher work efficiency of breeder in batch production, however, with no improvement in reproductive performance. [ABSTRACT FROM AUTHOR]

Background: Sows commonly experience insulin resistance in late gestation and lactation, causing lower feed intake and milk production, which can lead to higher mortality rates in newborn piglets. The probiotic Lactobacillus rhamnosus GG (LGG) is known to improve insulin resistance. However, whether supplementing LGG can improve insulin sensitivity in sows and enhance lactation performance, particularly the early survival of offspring remains unclear. Hence, we explored the effects and mechanisms of supplementing LGG during late gestation and lactation on sow insulin sensitivity, lactation performance, and offspring survival. In total, 20 sows were randomly allocated to an LGG (n = 10) and control group (n = 10). Results: In sows, LGG supplementation significantly improved insulin sensitivity during late gestation and lactation, increased feed intake, milk production and colostrum lactose levels in early lactation, and enhanced newborn piglet survival. Moreover, LGG treatment significantly reshaped the gut microbiota in sows, notably increasing microbiota diversity and enriching the relative abundance of insulin sensitivity-associated probiotics such as Lactobacillus, Bifidobacterium, and Bacteroides. Serum metabolite and amino acid profiling in late-gestation sows also revealed decreased branched-chain amino acid and kynurenine serum levels following LGG supplementation. Further analyses highlighted a correlation between mitigated insulin resistance in late pregnancy and lactation by LGG and gut microbiota reshaping and changes in serum amino acid metabolism. Furthermore, maternal LGG enhanced immunity in newborn piglets, reduced inflammation, and facilitated the establishment of a gut microbiota. Conclusions: We provide the first evidence that LGG mitigates insulin resistance in sows and enhances offspring survival by modulating the gut microbiota and amino acid metabolism. [ABSTRACT FROM AUTHOR]

The emerging risk of resistance to antimicrobials has brought about the need to document animal production in the European Union. The required documentation of treatment data on-farm may withhold viable information, which can be analysed if made accessible in a digital format. The aim of the study was to use a digital system for the documentation of treatments and to investigate relationships between treatments, performance parameters and breeds. Data were collected between August 2020 and September 2022 on a combined pig farm in northern Germany. A digital tool was used to obtain data on treatments, while sows' performance information and breed data were drawn from the sow planner of the farm. Treatment indices were generated for the sows (TIS, treatment index sow) and the sows' litters (treatment index litter, TIL). During the time of data collection, 17 % (n = 113) of the farrowing events (n = 661) took place with farrowing-related treatments (FRTs), and 43.5 % (n = 94) of the sows had to receive FRT at least once. Linear mixed models were used to investigate the dependent variables TIS, TIL and FRT and the performance parameters piglets born alive, stillborn piglets and weaned piglets. The explanatory variables parity, season, year, the interaction of season and year, and the breed of the sow (crossbred Large White × Landrace and purebred Large White), as well as the random effect of the sow nested in the breed of the sow, were included in the models for all dependent variables. Additionally, the breed of the boar (Duroc, Pietrain), TIS, TIL and FRT were investigated in the models of the performance parameters. The following effects were all significant (p<0.05). Parity had an effect on TIS, piglets born alive and stillborn piglets. Sows with parity of 1 had the highest least-squares mean (LSM) = 0.42 (standard error (SE) = 0.04) for TIS, which decreased over parities of 2–3, 4–5 and 6–7 (LSM = 0.16, SE = 0.04) and increased for parity of ≥8 (LSM = 0.34, SE = 0.05). While sows with parity of 1 produced the lowest numbers and sows with parities of 4–5 the highest numbers of piglets born alive, the number of stillborn piglets increased with parity number, with the lowest numbers for parity of 1 and the highest numbers for parity of ≥8. Overall, the performance of the farm improved in the second year of data collection with less FRT, more weaned piglets and fewer stillborn piglets. Crossbred sows produced more piglets born alive and weaned piglets but also more stillborn piglets. The use of Pietrain boars led to more piglets being born alive. FRT and high treatment indices of the sow related with an increase in stillborn piglets and high treatment indices of the sow resulted in fewer piglets born alive. In conclusion, digitalized treatment data allow for analyses of the herd's health status, and relations between production performance and treatments can be investigated. [ABSTRACT FROM AUTHOR]

Simple Summary: Modern sows face increased nutritional challenges due to inadequate feeding programs causing excessive maternal tissue loss and reproductive failure. Rapid genetic improvements in reproductive performance should be nutritionally supported; however, current feeding programs have limitations to support the needs of modern sows. Litter size at birth and the birthweight of piglets have increased, increasing the nutrient needs for sows during gestation and lactation. This review also addresses physiological challenges facing modern sows including high oxidative stress, pelvic organ prolapse, and lameness as well as negative impacts on colostrum and milk quality. To mitigate these challenges, there is growing interest in investigating the functional roles of select bioactive compounds as feed additives. Such bioactive compounds have been utilized to reduce disease and illness related to physical stressors, improve colostrum and milk quality, and support sow intestinal health. This review demonstrates that the feeding of modern sows poses unique challenges for nutritionists and the rapid genetic improvements to reproductive performance warrants updated feeding programs and selective use of bioactive compounds. The objectives of this review are to identify the nutritional challenges faced by modern sows and present potential solutions to mitigate excessive maternal tissue loss and reproductive failure as it relates to recent genetic improvements. Current feeding programs have limitations to support the rapid genetic improvements in reproductive performance for modern sows. Since 2012, both litter size at birth and fetal weight have increased by 2.26 pigs per litter and 0.22 kg per piglet, respectively, thereby increasing the nutrient needs for sows during gestation and lactation. Prediction models generated in this review predict that modern sows would need 31% more lysine during gestation when compared with current feeding programs. Physiological challenges facing modern sows are also addressed in this review. High oxidative stress, pelvic organ prolapse, and lameness can directly affect the sow, whereas these physiological challenges can have negative impacts on colostrum and milk quality. In response, there is growing interest in investigating the functional roles of select bioactive compounds as feed additives to mitigate the severity of these challenges. Selenium sources, catechins, and select plant extracts have been utilized to reduce oxidative stress, calcium chloride and phytase have been used to mitigate pelvic organ prolapse and lameness, algae and yeast derivatives have been used to improve colostrum and milk quality, and fiber sources and probiotics have been commonly utilized to improve sow intestinal health. Collectively, this review demonstrates the unique challenges associated with managing the feeding programs for modern sows and the opportunities for revision of the amino acid requirements as well as the use of select bioactive compounds to improve reproductive performance. [ABSTRACT FROM AUTHOR]

Simple Summary: Today, sows produce larger litter sizes than they did several years ago. Modern hyperprolific sows with greater parity numbers experience longer farrowing durations and require more farrowing assistance compared to those with lower parity numbers. Among reproductive hormones, oxytocin plays a critical role during parturition and is often given to sows during farrowing to shorten its duration. Oxytocin is a uterotonic hormone originating from the hypothalamus and released from the posterior pituitary gland, and it is important for the expulsion of fetuses. Aging is a well-known factor that affects productivity and increases farrowing duration in modern prolific sows. In the current study, we demonstrated a decrease in the immunolocalization of oxytocin receptors in the uterine tissue of older sows, supporting a previous study that showed weaker uterine contractions in sows with a greater parity number, which possibly affects the duration of farrowing. This study investigated the gross morphology of reproductive organs and oxytocin receptor distribution across different parities in hyperprolific sows. A total of thirty-two reproductive organs from Landrace × Yorkshire crossbred sows were categorized into three groups based on parity numbers: 1 (n = 10), 2–5 (n = 12), and ≥6 (n = 10). All sows were culled due to management problems, and none had reproductive disorders. A gross morphology examination of the ovaries, uterus, and the rest of the reproductive tract was conducted. Using immunohistochemistry, the levels of oxytocin receptor were evaluated in five layers of the uterus, the epithelial, superficial glandular, deep glandular, and circular and longitudinal smooth muscles of the myometrium, and were quantified using an H-score. On average, the age and body weight of sows and the total number of piglets born per litter were 799.8 ± 327.8 days, 213.2 ± 31.7 kg, and 15.5 ± 4.8, respectively. The numbers of ovulations in sows in parity number 1 (19.9 ± 2.4) were lower than those in sows in parity numbers 2–5 (29.7 ± 2.0, p = 0.004) and ≥6 (27.7 ± 2.1, p = 0.022). The uterine weights of sows in parity number 1 (902.9 ± 112.5 g) were lower than those of parity numbers 2–5 (1442.1 ± 111.8 g, p = 0.001) and ≥6 (1394.3 ± 125.1 g, p = 0.004). The length of the uterus in sows with parity number 1 (277.9 ± 26.1 cm) was shorter than those in sows with parity numbers 2–5 (354.6 ± 25.9 cm, p = 0.033) and tended to be shorter than those in sows with parity numbers ≥ 6 (346.6 ± 29.0 cm, p = 0.068). The immunolocalization of oxytocin receptors could be detected in various parts of the porcine endometrium and myometrium. Among the five tissue layers of the uterus, the H-score of oxytocin receptors in the deep uterine glands was greater than in the superficial uterine glands (p = 0.023) and the circular muscle layer of the myometrium (p = 0.011), but it did not differ from the epithelial layer of the endometrium (p = 0.428) or the longitudinal muscle layer of the myometrium (p = 0.081). Sows with parity numbers ≥ 6 had a lower oxytocin receptor H-score than those with parity numbers 1 (p < 0.001) and 2–5 (p < 0.001). In conclusion, these data emphasize the notable variations in several reproductive parameters and the levels of oxytocin receptor within the uterus of hyperprolific sows. Across the majority of uterine tissue layers, there was a marked decrease in the H-score of the oxytocin receptor in the older sows. [ABSTRACT FROM AUTHOR]

Introduction: This study was conducted to evaluate the effects of dietary galacto-oligosaccharides (GOS) and hyocholic acids (HCA) during late gestation and lactation on reproductive performance, colostrum quality, antioxidant capacity and gut microbiota in multiparous sows. Methods: A total of 60 healthy multiparous cross-bred sows (Landrace × Yorkshire) were randomly fed 4 groups diets as follows: the basal diets (CTRL group), or the basal diets containing only 600  mg/kg GOS (GOS group), 600  mg/ kg GOS  +  100  mg/kg HCA (GOS  +  Low HCA group), and 600  mg/kg  +  200  mg/ kg HCA (GOS  +  High HCA group) from d 85 of gestation to weaning. Multiple parameters of sows were determined. Results: There was a trend of shortening the labor process of sows (p  =  0.07) in the GOS group and GOS  +  Low/High HCA group. Compared with the CTRL group, the GOS  +  Low/High HCA group increased the average piglets weight at birth (p  <  0.05), and increased the IgA concentration of colostrum (p  <  0.05). In addition, serum triglyceride (TG) concentration was lower (p  <  0.05), and serum total antioxidant capacity (T-AOC) was higher (p  <  0.05) in the GOS and GOS  +  Low/High HCA groups than in the CTRL group at farrowing. Serum catalase (CAT) activities was higher in the GOS and GOS  +  High HCA groups than in the CTRL group at farrowing. The 16S rRNA analysis showed that GOS combination with high-dose HCA shaped the composition of gut microbiota in different reproductive stages (d 107 of gestation, G107; d 0 of lactation, L0; d 7 of lactation, L7). At the phylum level, the relative abundance of Bacteroidota and Desulfobacterota in G107, Bacteroidota, and Proteobacteria in L0, and Planctomycetota in L7 was increased in GOS  +  High HCA group (p  <  0.05). Spearman correlation analysis showed that Streptococcus was positively correlated with the serum TG but negatively correlated with the average piglets weight at birth (p  <  0.05). Conclusion: This investigation demonstrated that the administration of galactooligosaccharides (GOS) in conjunction with hyocholic acids (HCA), to sows with nutrient restrictions during late gestation and lactation, further improved their antioxidant capacity and milk quality. The observed beneficial effects of GOS  +  HCA supplementation could potentially be linked to an improvement in gut microbiota disorders of the sows. [ABSTRACT FROM AUTHOR]

Simple Summary: At present, it is particularly important to reduce costs and improve economic efficiency in farm production. However, in intensive production, the situation of insufficient milk production in sows and the poor growth of piglets has become increasingly serious. In this context, how to increase sow milk production and improve piglet growth is the primary goal of researchers. Some studies have shown that feeding MSG to sows can enhance the growth of newborn pigs, but some researchers suspect that the addition of MSG is not safe for sows. Therefore, this experiment aims to study the impact of MSG on sows and piglets. Our research results prove that MSG is safe and effective, can reduce costs, improve economic benefits, and is suitable for intensive production. In most current farm operations, lactating sows need to overcome reproductive and environmental stresses that have resulted in poor sow production performance and piglet growth. Therefore, this study aimed to investigate the effects of in-feed supplementation of monosodium glutamate (MSG) in sows during late gestation lactation in regard to litter performance. The study subjects were 12 multi-parity sows (Landrace × Large White), farrowing sows with an average parity of four (three with three parities, seven with four parities, and two with five parities). They were randomly divided into the following two diet groups: the basal diet as a control (CON) group based on corn and soybean meal; and the basal diet + 2% MSG group. The experimental time ranged from 109 days before delivery to 21 days after delivery. There were six sows in each group, and each sow served as the experimental unit. There were no significant differences (p > 0.05) in body weight (BW), back fat (BF) thickness and estrus interval between sows supplemented with 2% MSG in their diets before and after farrowing and during weaning (p > 0.05). However, MSG-treated sows tended to increase BW loss at farrowing more than the CON group (p = 0.093) but lost less weight during lactation than the CON group (p = 0.019). There were no significant differences in the body condition scores (BCSs) and BF loss of the two groups of sows before and after farrowing and at weaning (p > 0.05). There was no significant difference in the weight of newborn piglets between the two groups of sows (p > 0.05). The weaning weight (p = 0.020) and average daily gain (ADG) (p = 0.045) of suckling piglets were higher in the MSG treated group compared to the CON group. The daily milk production of sows in the MSG treatment group was higher compared to the CON group (p = 0.045). The protein concentration of milk at week 3 (p = 0.060) and fat concentration of milk at week 5 (p = 0.095) of the MSG-supplemented sows tended to increase more than the CON group. In summary, the dietary inclusion of MSG supplementation had a beneficial effect on the late gestating sows and their piglet's growth and milk production. Our research has shown that the addition of 2% MSG in late gestation and lactation diet would be beneficial for both sow and piglet production. [ABSTRACT FROM AUTHOR]

Background: Immediately after birth, newborn piglets fight to establish a teat order. During this process, lesions appear on the piglets' faces and on the sows' teats, which is why tooth resection is carried out on many farms in Germany even though it is known that this procedure is frequently resulting in pulp openings. The opening of a pulp cave is suspected to cause painful tooth alterations and may be an entrance for infectious agents. The purpose of this study was to analyse the effect of tooth resection on skin lesions, development of bodyweight and behaviour in suckling piglets. Four days prepartum, 110 sows in farrow-to-finish production were assigned to one of three treatments. Litters had their teeth left intact (control group, CG), ground with a tea-cup roller head (Tea-cup head grinder group, TCG, Wilofa Diamant, D-56,133 Fachbach, Germany) or ground with a diamond rolling head (rolling head grinder group, RG, IBS/E Company Proxxon GmbH, 54,343 Föhren, Germany). The number of pulp openings in the RG and TCG was examined using a random sample. Piglet body weight and skin lesion scores were recorded within the first 24 h after birth and during each week of the suckling period. Each sow's udder was examined before farrowing, in the second week of lactation and at weaning. The behaviour of the litters from nine sows was video-recorded throughout the suckling period. The aim of this study was to investigate the effects of tooth grinding by a tea-cup head (compared to grinding by a diamond roller head and no grinding [control group]) on the behaviour and average daily gain of piglets as well as on skin lesions on sow udder. Results: The number of dental injuries was significantly greater in the RG than in the TCG (p < 0.01). Head lesions on piglets were significantly more common in the CG than in the RG (p = 0. 02). Compared to CG piglets, TCG piglets had a significantly greater weight at the end of the suckling period (p = 0.02). No significant difference between treatments was found in the sows' udder (parenchyma, skin, or teat) or in the behaviour of the litters. Conclusion: As tooth grinding is frequently inducing pulp openings, the necessity of the procedure should be carefully and critically scrutinised. In case tooth resection seems inevitable until the underlying management problems have been solved, the Tea-cup grinding head should be used due to significantly fewer pulp openings. [ABSTRACT FROM AUTHOR]

Background: Longitudinal records of automatically-recorded vaginal temperature (TV) could be a key source of data for deriving novel indicators of climatic resilience (CR) for breeding more resilient pigs, especially during lactation when sows are at an increased risk of suffering from heat stress (HS). Therefore, we derived 15 CR indicators based on the variability in TV in lactating sows and estimated their genetic parameters. We also investigated their genetic relationship with sows' key reproductive traits. Results: The heritability estimates of the CR traits ranged from 0.000 ± 0.000 for slope for decreased rate of TV (SlopeDe) to 0.291 ± 0.047 for sum of TV values below the HS threshold (HSUB). Moderate to high genetic correlations (from 0.508 ± 0.056 to 0.998 ± 0.137) and Spearman rank correlations (from 0.431 to 1.000) between genomic estimated breeding values (GEBV) were observed for five CR indicators, i.e. HS duration (HSD), the normalized median multiplied by normalized variance (Nor_medvar), the highest TV value of each measurement day for each individual (MaxTv), and the sum of the TV values above (HSUA) and below (HSUB) the HS threshold. These five CR indicators were lowly to moderately genetically correlated with shoulder skin surface temperature (from 0.139 ± 0.008 to 0.478 ± 0.048) and respiration rate (from 0.079 ± 0.011 to 0.502 ± 0.098). The genetic correlations between these five selected CR indicators and sow reproductive performance traits ranged from − 0.733 to − 0.175 for total number of piglets born alive, from − 0.733 to − 0.175 for total number of piglets born, and from − 0.434 to − 0.169 for number of pigs weaned. The individuals with the highest GEBV (most climate-sensitive) had higher mean skin surface temperature, respiration rate (RR), panting score (PS), and hair density, but had lower mean body condition scores compared to those with the lowest GEBV (most climate-resilient). Conclusions: Most of the CR indicators evaluated are heritable with substantial additive genetic variance. Five of them, i.e. HSD, MaxTv, HSUA, HSUB, and Nor_medvar share similar underlying genetic mechanisms. In addition, individuals with higher CR indicators are more likely to exhibit better HS-related physiological responses, higher body condition scores, and improved reproductive performance under hot conditions. These findings highlight the potential benefits of genetically selecting more heat-tolerant individuals based on CR indicators. [ABSTRACT FROM AUTHOR]

Influenza A virus (IAV) infections in swine are usually subclinical, but they can reach high morbidity rates. The mortality rate is normally low. In this study, six vaccinated, spontaneously deceased sows revealed IAV infection and enhanced neutrophilic bronchopneumonia with unexpectedly large numbers of infiltrating eosinophils. The purpose of this study was to characterize these lung lesions with special emphasis on the phenotypes of inflammatory cells, the presence of eosinophilic peroxidase (EPO), and neutrophil extracellular traps (NETs). The number of Sirius red-stained eosinophils was significantly higher in the lungs of IAV-infected sows compared to healthy pigs, indicating a migration of eosinophils from blood vessels into the lung tissue stimulated by IAV infection. The detection of intra- and extracellular EPO in the lungs suggests its contribution to pulmonary damage. The presence of CD3+ T lymphocytes, CD20+ B lymphocytes, and Iba-1+ macrophages indicates the involvement of cell-mediated immune responses in disease progression. Furthermore, high numbers of myeloperoxidase-positive cells were detected. However, DNA-histone-1 complexes were reduced in IAV-infected sows, leading to the hypothesis that NETs are not formed in the IAV-infected sows. In conclusion, our findings in the lungs of IAV-infected vaccinated sows suggest the presence of so far unreported field cases of vaccine-associated enhanced respiratory disease. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the effects of a polyphenolic phytogenic feed additive (PFA) based on plant extracts, such as Embelia officinalis, Ocimum sanctum and nut fibre, on the redox status, haematological parameters, and piglet mortality in sows. A total of 64 primiparous sows were divided into two groups: T1-control group: regular gestation (GF) and lactation feed (LF), T2 group: regular GF and LF supplemented with a PFA (10 g daily) for 14 days before and 7 days after the farrowing. Blood samples were collected 0–3 h after farrowing. Haematological parameters (Packed Cell Volume/PCV, White Blood Cells/WBC, Platelets/PLTs) were counted in blood smears. Thiobarbituric acid reactive substances (TBARS) and protein carbonyls (CARBS) levels were determined in sow plasma. The performance and reproductive parameters of sows at farrowing and weaning days were recorded. The mean numbers of PCV and PLT counts in the T2 group were higher in comparison to the T1 group (p = 0.041, p = 0.033, respectively). In contrast, the mean numbers of WBC and neutrophils were almost significantly higher in the T2 group (p = 0.051). The mean number of stillborn piglets was significantly higher in the T1 group (2.12) compared to the T2 group (1.03). The mean number of alive piglets 24 h after farrowing and the mean number of the weaned piglets were significantly higher in group T2 (13.9 vs. 15.4 and 12.6 vs. 14.3). Sows in group T2 had significantly more backfat at weaning than the sows in group T1 (13.3 vs. 12.7). The mean levels of CARBS (nmol/mL) and TBARS (μmol/L) in group T1 (24.8 and 18.7) were significantly higher in comparison to group T2 (18.3 and 14.9). In conclusion, the use of a polyphenolic PFA in sows has beneficial effects on their welfare and performance due to its antioxidative effects. Furthermore, PFAs appear to exert antithrombotic, anti-inflammatory, and protective effects on PLTs, WBCs, and RBCs, respectively. [ABSTRACT FROM AUTHOR]

Simple Summary: Handling of large litters in hyperprolific pig farms can only be done using a nurse sow system or artificial rearing system. Nurse sows are a group of sows used to nurse surplus piglets via extended lactation length. Understanding the husbandry aspects of the nurse sow system is required for improving pig farming practices. Furthermore, removal patterns focusing on nurse sows have not been carried out, and therefore, this research provides information that contributes to how culling decisions could be made in herds utilizing nurse sows. A high removal of young parity nurse sows affects herd structure. After weaning, nurse sows are highly removed due to failure to return to cycle and conceive. Return to estrus is considered the most important indicator of reproductive soundness of any weaned sow and therefore a crucial component in managing non-productive days in herds. Extended lactation length for nurse sows of two weeks appears to be the most appropriate period in which the nurse sow experiences a lesser chance of removal due to failure to express heat. This study aimed to characterize and quantify reasons for the removal of nurse sows and identify the removal associated with their extended lactation length (ELL). A total of 100,756 removed nurse sows within a period of 2016–2022 from 53 sow herds in the Midwest USA were analyzed. Reproductive failure was the most common removal reason (χ2 = 8748.421, p < 0.001) affecting P1, P2, and P3 nurse sows. Failure to conceive and absence of estrus were the main causes of reproductive failure (χ2 = 352.480, p < 0.001) affecting P1 and P2 nurse sows and P1 and P5 nurse sows, respectively. When P2 and P6 nurse sows had an ELL of 0–7 d, they faced a high chance (χ2 = 13.312, p = 0.021) of removal due to conception failure and failure to return to heat, respectively. When P2 and P5 nurse sows had an ELL of 8–14 d, they were highly vulnerable (χ2 = 59.847, p < 0.001) to removal due to failure to conceive and showing heat, respectively. Finally, when ELL was at 15–21 days, P4 and P5 nurse sows were more likely (χ2 = 41.751, p < 0.001) to be removed due to failure to express heat, whereas at the same time, P2 and P3 nurse sows experienced the same removal threat due to failing to conceive. These results could help producers manage nurse sow systems. [ABSTRACT FROM AUTHOR]

Simple Summary: Maintaining the health of sows in pig production poses a persistent and significant challenge that directly impacts sow reproductive performance as well as producer economics. Pectin, a dietary fiber with immense potential, was investigated in this study for its potential to enhance the health of pregnant sows. Throughout gestation, we supplemented the basic diet with 3% of low-ester pectin to examine its potential effects in pregnant sows. We collected and analyzed serum and fecal samples from the sows to evaluate their fecal microbiota, serum metabolites, and cytokines, aiming to provide fundamental data for pectin utilization in animal production. This study aimed to investigate the effects of the dietary fiber pectin on the gut microbiota and health of parturient sows. A total of 30 parity 5–7, multiparous gestation sows (Large White × Landrace) were randomly assigned to two treatment groups after mating: Con (control, basic diet) and Pec (pectin, 3%). The sows received the two diets during gestation, and all sows were fed the same standard basic diet during lactation. The results of β-diversity showed that the composition of the gut microbiota was different in the Con and Pec groups. Compared with the sows in the Con group, the Pec sows showed a higher abundance of the gut bacteria Clostridium and Romboutsia and a lower abundance of harmful bacteria (Micrococcaceae, Coriobacteriaceae, Dorea, Actinomyces). On the other hand, the SCFA plasma concentration was increased in the Pec group, while pro-inflammatory cytokine (IL-6, IL-1β, and TNF-α) concentrations were decreased. In conclusion, the soluble dietary fiber pectin could improve the reproductive performance and health of sows by increasing the abundance of some commensal bacteria enhancing the metabolite SCFA levels and reducing the pro-inflammatory cytokine plasma levels. [ABSTRACT FROM AUTHOR]

It is possible to identify sub-populations of sows in every pig herd that consistently give birth to low birth weight (BW) piglets, irrespective of the litter size. A previous study from our group demonstrated that placental development is a main factor affecting the litter birth weight phenotype (LBWP) in sows, thereby impacting the BW of entire litters, but the biological and molecular pathways behind this phenomenon are largely unknown. The aim of this study was to investigate the differential gene expression in placental tissues at day 30 of gestation between low LBWP (LLBWP) vs. high LBWP (HLBWP) sows from a purebred Large White maternal line. Using mRNA sequencing, we found 45 differentially expressed genes (DEGs) in placental tissues of LLBWP and HLBWP sows. Furthermore, (GO) enrichment of upregulated DEGs predicted that there were two biological processes significantly related to cornification and regulation of cell population proliferation. To better understand the molecular interaction between cell proliferation and cornification, we conducted transcriptional factor binding site (TFBS) prediction analysis. The results indicated that a highly significant TFBS was located at the 5′ upstream of all four upregulated genes (CDSN, DSG3, KLK14, KRT17), recognized by transcription factors EGR4 and FOSL1. Our findings provide novel insight into how transcriptional regulation of two different biological processes interact in placental tissues of LLBWP sows. [ABSTRACT FROM AUTHOR]

Premating maternal nutrition is crucial for postweaning follicle growth, thereby influencing piglet birth weight in subsequent litters. The present study investigated the impact of supplementing a carbohydrate-rich premating diet in sows on metabolic hormones, subsequent piglet birth weight and reproductive performance. Sows were distributed into three groups, control (n=42) received standard diets; treatment I (n=41) received the same diets supplemented with 500 g of a carbohydrate-rich premating diet from weaning until insemination; treatment II (n=42) received the same diets supplemented with 500 g of a carbohydrate-rich premating diet from 7 days before weaning until insemination. Blood samples were taken from sows around weaning to measure serum insulin-like growth factor-1 and insulin, and blood glucose after feeding. The study found that sows on a carbohydrate-rich diet (treatment II) had higher postprandial glucose (P<0.05) and insulin levels (P=0.06) than others. This diet did not affect overall reproductive performance, but it did increase piglet birth weight and reduce the number of low-birth weight piglets compared to the control (P<0.001) and treatment I groups (P<0.05). Supplementing a carbohydrate-rich premating diet for 7 days before weaning until insemination enhanced postprandial glucose and insulin concentrations in weaned sows. This dietary intervention led to improved subsequent piglet birth weight and reduced the proportion of low-birth weight piglets. [ABSTRACT FROM AUTHOR]

A field experiment was conducted in 2016-17 and 2017-18 during Rabi season at Regional Agricultural Research Station, Palem, Nagarkurnool District of Telangana State, India. The treatments consisted of 7 sowing dates with 15-day intervals (T1- October 10th, T2- October 25th, T3- November 10th, T4- November 25th, T5- December 10th, T6- December 25th and T7- January 10th). The experiment was laid out in a randomized block design and was replicated three times. This study was conducted with the objective of determining the optimum sowing time for quinoa in Telangana state, India. A greater seed yield was detected for the crops sown between October 10th (2174 kg/ha) and November 25th (1931 kg/ha); on further delay in sowing after November 25th,i.e., December 10th (1026 kg/ha), December 25th (600 kg/ha) and January 10th(590 kg/ha), the seed yields were drastically reduced due to the higher temperature at the reproductive stage of the crop. Hence, from this study, it can be noted that the optimum sowing date for quinoa in Telangana was from October 10th to November 25th. [ABSTRACT FROM AUTHOR]

Acquiring real-time feeding information for monitoring lactating sows and their feeding requirements is a challenging task. Real-time data represent an important input for numerous tasks, such as disease monitoring, nutritional regulation, and feeding modeling. However, concurrently monitoring large numbers of sows and processing the real-time information for modeling is challenging using existing platforms. In this paper, we describe the design and development of a system that monitors and processes sows' feed and water consumption in real time. The system was custom-developed using open-source networking technologies. The system consists of three components: an electronic sow feeder connected to a central controller via a CAN network, an MQTT service cluster, and a data processing program. The MQTT service cluster uses Netty to develop a single service node, and it uses Zookeeper and Redis to complete node registration, discovery, and scheduling. The data processing program is based on Spark and Flink. We conducted comparative testing of three common codecs (Java Serializer, Marshalling, and Protostuff) to further speed up data transmission. The results of the experiment show that, with three service nodes, the system can concurrently monitor up to 20,000 sows. Moreover, the system achieves optimal performance when monitoring 10,000 sows at the same time, with a TPS of 6399 pcs/s and an RT of 643 ms. [ABSTRACT FROM AUTHOR]

To boost productivity in pig production, it is essential to improve the litter size per sow. However, achieving this goal requires reducing newborn mortality rates and increasing their weight gain. In this regard, prioritizing animal welfare and adopting practices that promote sustainability in the activity is crucial. The study aimed to evaluate the sustainable flooring coverage of farrowing crates, considering piglet adaptation, thermal comfort, animal development, and increased productivity. Three types of flooring treatments were defined: T1—wood shavings; T2—solid rubber mat; T3—perforated rubber mat, for 12 litters (±50 animals/treatment). Behavior, physiology, piglet production indices, and facility microclimate were assessed. The facility's microclimate indicated the possibility of cold stress. There was a greater preference for the mat treatments among the animals, as they remained on them for longer periods and showed increased activity in the farrowing crates. The presence of mats resulted in lower mortality due to crushing, positively contributing to production. These findings suggest the potential of using recyclable rubber mats in pig production and encourage the exploration of new designs and installation locations. [ABSTRACT FROM AUTHOR]

Maternal dietary supplementation with chitosan oligosaccharide (COS) has been considered as a potential intervention to mitigate the occurrence of intrauterine growth restriction (IUGR) and improve postnatal growth. The present study investigated the effect of COS as a dietary supplement for sows during late gestation and lactation on their productivity, placental function, and the intestinal health of IUGR piglets. From day (d) 85 of late gestation to d 21 of lactation, 30 sows were randomly divided into either a control group (basal diet) or a COS group (basal diet + 100 mg kg−1 COS). At d 21 of lactation, eight normal and eight IUGR littermates from eight litters belong to control sows, as well as eight IUGR littermates from COS sows, were selected for further analysis. The results showed a significant reduction in the number of stillbirths and mummies in COS groups (p

Abstract The aim of this study was to classify the sows for longevity based on the total number of piglets born alive in the first parities. Data comprising the period from January 2017 to March 2020 were obtained from a commercial farm with 5,500 sows. A total of 8,869 sows were used in the analyses. The animals were divided into three parity groups according to the 1st and 3rd quartiles of the total number of piglets born alive in the first and second farrowing: parity 1 - 1L (low): 16 piglets; parity 2 - 2L: 16 piglets. Sows exhibiting high performance in the first parity continued to do so up to the sixth parity. Regarding parity 1 classification, 1H sows (>16 piglets) produced more piglets than the other classes up to the fifth parity. When the parity 2 classification was added, 1H2H and 1H2M sows produced more piglets up to the fourth parity. Comparison of the length of stay of sows in the herd showed a longer mean stay of the 1H2M class. The main reasons for culling were cystitis, uterine prolapse, hoof problems, and physical condition. In general, the results demonstrated that it is possible to use the number of piglets born alive in the first two parities of sows to predict the productivity of subsequent parities. The method is able may therefore help to maintenance of the sow in the herd or culling of sows.