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3. A Novel 3-O-rhamnoside: 2″-O-xylosyltransferase Responsible for Terminal Modification of Prenylflavonol Glycosides in Epimedium pubescens Maxim.

Author

Yao, Yu, primary, Gu, Jiajun, additional, Luo, Yanjiao, additional, Zhang, Yixin, additional, Wang, Yuanyue, additional, Pang, Yongzhen, additional, Jia, Shangang, additional, Xu, Chaoqun, additional, Li, Doudou, additional, Suo, Fengmei, additional, Shen, Guoan, additional, and Guo, Baolin, additional

Published
2022
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14. Natural Resource Monitoring ofRheum tanguticumby Multilevel Remote Sensing

Author

Xie, Caixiang, primary, Song, Jingyuan, additional, Suo, Fengmei, additional, Li, Xiwen, additional, Li, Ying, additional, Yu, Hua, additional, Xu, Xiaolan, additional, Luo, Kun, additional, Li, Qiushi, additional, Xin, Tianyi, additional, Guan, Meng, additional, Xu, Xiuhai, additional, Miki, Eiji, additional, Takeda, Osami, additional, and Chen, Shilin, additional

Published
2014
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15. Comprehensive Analysis of Alternative Splicing in Digitalis purpurea by Strand-Specific RNA-Seq.

Author

Wu, Bin, Suo, Fengmei, Lei, Wanjun, and Gu, Lianfeng

Subjects
*ALTERNATIVE RNA splicing, *PURPLE foxglove, *AMINO acid sequence, *MEDICINAL plants, *HEART failure treatment, *GLYCOSYLTRANSFERASES, *MONOOXYGENASES
Abstract

Digitalis purpurea (D. purpurea) is one of the most important medicinal plants and is well known in the treatment of heart failure because of the cardiac glycosides that are its main active compounds. However, in the absence of strand specific sequencing information, the post-transcriptional mechanism of gene regulation in D. purpurea thus far remains unknown. In this study, a strand-specific RNA-Seq library was constructed and sequenced using Illumina HiSeq platforms to characterize the transcriptome of D. purpurea with a focus on alternative splicing (AS) events and the effect of AS on protein domains. De novo RNA-Seq assembly resulted in 48,475 genes. Based on the assembled transcripts, we reported a list of 3,265 AS genes, including 5,408 AS events in D. purpurea. Interestingly, both glycosyltransferases and monooxygenase, which were involved in the biosynthesis of cardiac glycosides, are regulated by AS. A total of 2,422 AS events occurred in coding regions, and 959 AS events were located in the regions of 882 unique protein domains, which could affect protein function. This D. purpurea transcriptome study substantially increased the expressed sequence resource and presented a better understanding of post-transcriptional regulation to further facilitate the medicinal applications of D. purpurea for human health. [ABSTRACT FROM AUTHOR]

Published
2014
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16. Natural Resource Monitoring of Rheum tanguticum by Multilevel Remote Sensing.

Author

Xie C, Song J, Suo F, Li X, Li Y, Yu H, Xu X, Luo K, Li Q, Xin T, Guan M, Xu X, Miki E, Takeda O, and Chen S

Abstract

Remote sensing has been extensively applied in agriculture for its objectiveness and promptness. However, few applications are available for monitoring natural medicinal plants. In the paper, a multilevel monitoring system, which includes satellite and aerial remote sensing, as well as ground investigation, was initially proposed to monitor natural Rheum tanguticum resource in Baihe Pasture, Zoige County, Sichuan Province. The amount of R. tanguticum from images is M = S*ρ and S is vegetation coverage obtained by satellite imaging, whereas ρ is R. tanguticum density obtained by low-altitude imaging. Only the R. tanguticum which coverages exceeded 1 m(2) could be recognized from the remote sensing image because of the 0.1 m resolution of the remote sensing image (called effective resource at that moment), and the results of ground investigation represented the amounts of R. tanguticum resource in all sizes (called the future resource). The data in paper showed that the present available amount of R. tanguticum accounted for 4% to 5% of the total quantity. The quantity information and the population structure of R. tanguticum in the Baihe Pasture were initially confirmed by this system. It is feasible to monitor the quantitative distribution for natural medicinal plants with scattered distribution.

Published
2014
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