2 results on '"Szili, Noémi"'
Search Results
2. Regulation of PI-2b Pilus Expression in Hypervirulent Streptococcus agalactiae ST-17 BM110
- Author
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Périchon, Bruno, Szili, Noémi, du Merle, Laurence, Rosinski-Chupin, Isabelle, Gominet, Myriam, Bellais, Samuel, Poyart, Claire, Trieu-Cuot, Patrick, Dramsi, Shaynoor, Bidault, Floran, Biologie des Bactéries pathogènes à Gram-positif, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Ecologie et Evolution de la Résistance aux Antibiotiques / Ecology and Evolution of Antibiotics Resistance (EERA), Institut Pasteur [Paris] (IP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Sud Orsay-Centre National de la Recherche Scientifique (CNRS), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), This work was supported by the DIM Malinf from the Conseil Régional d'Ile-de-France (Grant DIM130065) to CP and SD. NS was recipient of a doctoral fellowship from the DIM Malinf. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., Université Paris-Sud - Paris 11 (UP11)-Institut Pasteur [Paris] (IP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Sud Orsay-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] - Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11) - Institut Pasteur [Paris] - Assistance publique - Hôpitaux de Paris (AP-HP) - Centre National de la Recherche Scientifique (CNRS), Institut Cochin (UM3 (UMR 8104 / U1016)), and Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Centre National de la Recherche Scientifique (CNRS)
- Subjects
Transcription, Genetic ,MESH: Codon, Initiator ,[SDV]Life Sciences [q-bio] ,Codon, Initiator ,lcsh:Medicine ,MESH: Virulence ,Biochemistry ,Nucleic Acids ,Lactococcus ,lcsh:Science ,Pathology and laboratory medicine ,Virulence ,Genomics ,Medical microbiology ,[SDV] Life Sciences [q-bio] ,Group B streptococci ,Fimbriae Proteins ,Pathogens ,Cellular Structures and Organelles ,MESH: Genes, Bacterial ,Lactococcus Lactis ,Research Article ,Pathogen Motility ,MESH: Operon ,Virulence Factors ,DNA transcription ,Microbiology ,Streptococcus agalactiae ,MESH: Fimbriae, Bacterial ,Operon ,Genetics ,Operons ,Gene Prediction ,Medicine and health sciences ,Bacteria ,MESH: Transcription, Genetic ,lcsh:R ,Organisms ,Biology and Life Sciences ,Streptococcus ,Computational Biology ,Cell Biology ,DNA ,Genome Analysis ,MESH: Fimbriae Proteins ,MESH: Streptococcus agalactiae ,Microbial pathogens ,Pili and Fimbriae ,Genetic Loci ,Genes, Bacterial ,MESH: Gene Deletion ,Fimbriae, Bacterial ,Bacterial pathogens ,lcsh:Q ,Gene expression ,Gene Deletion - Abstract
International audience; The widely spread Streptococcus agalactiae (also known as Group B Streptococcus, GBS) "hypervirulent" ST17 clone is strongly associated with neonatal meningitis. The PI-2b locus is mainly found in ST17 strains but is also present in a few non ST17 human isolates such as the ST-7 prototype strain A909. Here, we analysed the expression of the PI-2b pilus in the ST17 strain BM110 as compared to the non ST17 A909. Comparative genome analyses revealed the presence of a 43-base pair (bp) hairpin-like structure in the upstream region of PI-2b operon in all 26 ST17 genomes, which was absent in the 8 non-ST17 strains carrying the PI-2b locus. Deletion of this 43-bp sequence in strain BM110 resulted in a 3-to 5-fold increased transcription of PI-2b. Characterization of PI-2b promoter region in A909 and BM110 strains was carried out by RNAseq, primer extension, qRT-PCR and transcriptional fusions with gfpas reporter gene. Our results indicate the presence of a single promoter (Ppi2b) with a transcriptional start site (TSS) mapped 37 bases upstream of the start codon of the first PI-2b gene. The large operon of 16 genes located upstream of PI-2b codes for the group B carbohydrate (also known as antigen B), a major constituent of the bacterial cell wall. We showed that the hairpin sequence located between antigen B and PI-2b operons is a transcriptional terminator. In A909, increased expression of PI-2b probably results from read-through transcription from antigen B operon. In addition, we showed that an extended 5′ promoter region is required for maximal transcription of gfpas a reporter gene in S. agalactiae from Ppi2b promoter. Gene reporter assays performed in Lactococcus lactis strain NZ9000, a related non-pathogenic Gram-positive species, revealed that GBS-specific regulatory factors are required to drive PI-2b transcription. PI-2b expression is up-regulated in the BM110AcovR mutant as compared to the parental BM110 strain, but this effect is probably indirect. Collectively, our results indicate that PI-2b expression is regulated in GBS ST17 strains, which may confer a selective advantage in the human host either by reducing host immune responses and/or increasing their dissemination potential.
- Published
- 2017
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