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Pulp and paper industry is traditionally known to be a large contributor to environmental pollution due its large consumption of energy and chemicals. To reduce the chemical consumption, rate of delignification was increased by Schizophyllum commune in destructured sample of Dendrocalamus stictus, which was destructured by Impressafiner (compression- cum dewatering process). The extent of delignification was determined and comparison was made between the non-destructured and destructured samples. The influence of physical parameters like incubation time, moisture level, media, media concentration, pH and temperature were also examined during the study. It was found that rate of delignification was significantly 6.43% more in destructured sample than non-destructured sample. Kraft pulping of treated destructured sample shows 2.59 point reduction in kappa number than untreated non-destructured sample. Thus this paper provides an insight of the delignification extent in Dendrocalamus strictus after mechanical operation at varying physical parameters. [ABSTRACT FROM AUTHOR]