Search

Multiple sclerosis (MS) is a complicated, inflammatory disease that causes demyelination of the central nervous system (CNS), resulting in a variety of neurological abnormalities. Over the past several decades, different animal models have been used to replicate the clinical symptoms and neuropathology of MS. The experimental model of experimental autoimmune/allergic encephalomyelitis (EAE) and viral and toxin-induced model was widely used to investigate the clinical implications of MS. Discoidin domain receptor 1 (DDR1) signaling in oligodendrocytes (OL) brings a new dimension to our understanding of MS pathophysiology. DDR1 is effectively involved in the OL during neurodevelopment and remyelination. It has been linked to many cellular processes, including migration, invasion, proliferation, differentiation, and adhesion. However, the exact functional involvement of DDR1 in developing OL and myelinogenesis in the CNS remains undefined. In this review, we critically evaluate the current literature on DDR1 signaling in OL and its proliferation, migration, differentiation, and myelination mechanism in OL in association with the progression of MS. It increases our knowledge of DDR1 in OL as a novel target molecule for oligodendrocyte-associated diseases in the CNS, including MS.

The purpose of the study was to assess a phenolic compound that was extracted from the brown seaweed Enteromorpha intestinalis (Linn.), Nees (1820) for its antibacterial activities on different bacterial pathogens of fish illnesses. Hexane and methanol were utilized to extract bioactive compounds from the seaweed. Phytochemical analysis of the methnaolic extracts have revealed the presence of carbohydrates and glycosides, saponins, alkaloids, amino acids, flavonoids, fixed oil and fat, phenol compounds, tannins, and steroids. The bacterial pathogens of fish, including Providencia vermicola, Aeromonas hydrophila, Vibrio harveyi, and Aeromonas caviae, were targeted for antibacterial assessment. Among them, the methanolic crude extract and fraction-1 of E. intestinalis have shown maximum activity against A. hydrophila (22 ± 0.13 and 33 ± 0.56 µg/mL). Column chromatography was employed to isolate effective seaweed fraction, while FT-IR and HPLC were utilized for characterization. The phytochemical analysis confirmed the presence of various compounds in both extracts. The crude methanolic extract of E. intestinalis have demonstrated the maximum efficacy beside the tested bacterial pathogens. The FT-IR analysis revealed functional groups associated with polymeric structures in the seaweed extracts, including alcohols, phenols, aromatics, alkyl halides, aliphatic amines, alkynes, and alkenes. Overall, the methanolic extract of E. intestinalis exhibited significant antibacterial activity as a result of the phenolic chemicals that are present. The study provides valuable baseline data, emphasizing the need for further research towards developing antibacterial drug formulations.

Background: In recent years, various phytomedicines have been used in the treatment of hepatic disorders. The aim of this study is to find out whether Peltophorum pterocarpum bark and leaves can help rats that have been exposed to isoniazid and rifampicin-induced hepatotoxicity. Materials and Methods: The rats were divided into 10 groups each with 6 rats. The liver damage is induced by isoniazid and rifampicin. The leaf and bark of P. pterocarpum extracted with ethanol are freshly mixed in sterile water (100, 200 and 400 mg/kg body weight), and given to rats orally in the early morning as a single dosage per day until the study period. The animals were sacrificed and the tissue and serum samples were collected for further investigations. Results: The liver damage induced by isoniazid and rifampicin altered the various biochemical parameters levels, but after the treatment of P. pterocarpum barks and leaves the levels were significantly altered when compared to the negative control rats. The drug silymarin was used as a standard. Conclusion: The extracts have the protective effect of liver markers and membrane-bound enzymes against the toxin-treated rats. This result highlights the hepatoprotective properties of the leaves and barks of the plant in a similar manner and the formulation (Group 10) have high beneficial effects than other groups.

Colorectal cancer (CRC) is the third most prevalent cancer worldwide. Vitamin D receptor (VDR) gene mutations and Vitamin D deficiency contribute to CRC development and progression. Certain long non-coding RNAs (lncRNAs) directly inhibit VDR gene transcription, leading to VDR mutation. Thus, targeting oncogenic lncRNAs and VDR expression is a promising strategy for effective cancer treatment. Here, we green-synthesized Lactobacillus plantarum loaded nickel oxide nanoparticles (LpNiONPs) to assess their anticancer potential in CRC by targeting long non-coding RNA EIF3J- divergent transcript (lncRNA EIF3J-DT) and VDR. The potent bioactive component present in L. plantarum was identified via gas chromatography-mass spectrometry (GC–MS) analysis, and its interaction with VDR, as well as the functional interaction with lncRNA EIF3J-DT, were evaluated using the PyRx program and RPISeq-software, respectively. The LpNiONPs were characterized using UV–Vis spectroscopy, Zeta Potential, dynamic light scattering (DLS), fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray (EDX) and X-ray diffraction (XRD) techniques. The anticancer potential of LpNiONPs against HT-29 cells was assessed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, scratch assay, 4′,6-diamidino-2-phenylindole (DAPI)/ acridine orange-ethidium bromide (AO-EtBr) staining experiments, and reverse transcriptase-PCR to evaluate the expression of lncRNA EIF3J-DT/VDR and apoptotic-related genes. The potent bioactive compound Pyrrolo (1,2-a) pyrazine-1,4-dione in L. plantarum strongly interacts with VDR, highlighting its drug design potential. The formation of LpNiONPs was confirmed via UV–Vis spectroscopy with an absorption peak at 394 nm. LpNiONPs were positively charged, monodispersed, and stable square-shaped nanoparticles. LpNiONPs show dose-dependent cytotoxicity and induced apoptosis, confirmed by staining images in HT-29 cells. Moreover, LpNiONPs downregulated lncRNA EIF3J-DT, CYP24-A1 and BCL2 genes while upregulating VDR, cas-3, cas-9 and BAX in HT-29 cells. These findings suggest that LpNiONPs exhibit anticancer activity by promoting VDR-associated apoptosis by inhibiting lncRNA EIF3J-DT in CRC cells.

The rapid advancements in electric vehicle technology have elevated the lithium-ion battery to the forefront as the paramount energy storage solution. The battery’s health tends to deteriorate gradually as it ages. Due to the inevitable physiochemical reactions that take place inside the battery, it undergoes degradation and at a certain point, it becomes unserviceable. The battery degradation can be estimated using state of health (SOH). This paper employs data-driven techniques to estimate the state of health (SOH) of a battery. To estimate health parameters, a vast quantity of data, such as voltage, current, and temperature, is gathered from the NASA Prognostics Center of Excellence. The data is resampled using the superior Fourier Resampling method and then fed to a machine-learning algorithm. In this study, SOH estimation is carried out using three different machine-learning techniques i.e. Long Short Term Memory (LSTM), Deep Neural Networks (DNN), and Gated Recurrent Unit (GRU). However, the performance and accuracy of SOH estimation using these algorithms are highly dependent on hyperparameter tuning. Therefore, the optimal hyperparameter tuning has been adopted in the present work to reduce the time and complexity of the estimation. Further, the performance of various proposed techniques has been compared against each other using different performance indices such as root mean square error (RMSE), mean absolute error (MAE), and R-square error. GRU technique proved to be excelling with RMSE of 0.003, MAE of 0.003, and R-square error of 0.004 while estimating the SOH of various samples of batteries. This detailed analysis will be helpful for users to evaluate the performance of a battery and plan for maintenance accurately and effectively with minimum downtime.

Abstract The plant‐based medicine and diet is gaining importance in recent days. The consumption of Triticum aestivum grass in the form of juice and tablets is increasing among common people. The present study elaborates on the nutritional, antioxidant, and antimicrobial potential of a nongenetically modified type of T. aestivum grass, along with the evidence of molecular docking studies. The T. aestivum grass extracts like decoction, aqueous, ethanol, and chloroform were subjected to preliminary phytochemical tests, quantitative estimation, antioxidant analysis, and antimicrobial activity determination. The ethanolic extract that had good antioxidant and antimicrobial potential was subjected to gas column–mass spectroscopy (GC–MS) analysis and the compounds identified were docked against the antioxidant and antimicrobial receptors. The decoction and aqueous extracts performed well in preliminary qualitative tests with the presence of most of the phytochemicals tested. The decoction, aqueous, and ethanolic extracts possessed good concentrations of the phytochemicals. The decoction had about 210.83 ± 9.16 and 154.16 ± 0.33 mg/g of carbohydrates and proteins, respectively, while the aqueous extract had about 10.91 ± 0.08 mg/g of amino acids and the ethanolic extract had about 52.5 ± 1.4 mg/g of phenolic content, which were the highest concentration of the phytochemicals observed among the extracts. Along with phytochemical potential, good antioxidant potential in the DPPH and ABTS by decoction as well as ethanolic extract with nearly 40 and 90% inhibition, respectively, and in FRAP by aqueous extract with maximum OD value. The ethanolic extract exhibited the best inhibition potential against the Staphylococcus aureus about 28 ± 1 mm, Pseudomonas aeruginosa with 20 ± 2 mm, Bacillus cereus at 20 ± 1 mm by the ethanolic extract at 200 μg concentration, and Aspergillus fumigatus and A. niger at 15 ± 0 mm by the aqueous extract at 200 μg concentration. The GC–MS analysis revealed the presence of terpenoids, alkaloids, and phenols, which on docking had highest binding capacity toward the antioxidant and antimicrobial receptors.

Infertility has a remarkable global impact affecting approximately about 48 million couples worldwide. One of the most contended concerns in reproductive biology is the prospective influence of psychological stress on pregnancy rates. Individuals struggling to conceive face a stupendous amount of emotional turbulence and have a greater risk for psychological vulnerability. Both stress and infertility reinforce the impact of each other; hence, there exists a reciprocal relationship. Stress may be the major contributor to subsequent infertility. Infertility treatments may enhance stress levels as well as reduce treatment outcomes. The biological mechanisms that interlink stress and infertility are the outcome of the hormonal actions at the brain level, where they stimulate or suppress the hypothalamic-pituitary-adrenal axis (HPA) and have a potential influence on the secretion of the respective hormone by the reproductive organs and the pregnancy outcomes. Sex hormones play an essential role in reproductive biology as well as in general physiology where they generate the cycle and provide a potential environment for pregnancy. This article reviews the impact of stress on reproductive rates and the implications of sex hormones on infertility. Additionally, it suggests strategies to overcome the stress conditions and the scenarios that may lead to stress.

Introduction: The current healthcare system is insufficient to deal with the global impact of rising diabetes, necessitating the development of better alternatives, such as plant-derived natural compounds, to improve glucose tolerance. Therefore, the present study evaluated the phytoconstituents of the aqueous leaf and rhizome extracts of an unnoticed plant species, Kaempferia pulchra. In addition, the in vitro anti-hyperglycemic efficacy and antioxidant activities of the derived terpenoid fraction from the plant extracts were also assessed. Methods: The aqueous extracts of K. pulchra leaves and rhizomes were screened for phytoconstituents using gas chromatography-mass spectrometry. Colorimetric techniques were used to determine the terpenoid fraction’s total phenolic and flavonoid content. Terpenoids’ in vitro antioxidant properties were examined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (RSA) and ferric reducing power assay, proceeded by α-amylase and α-glucosidase inhibition tests, and anti-hyperglycemic potential was determined utilizing the terpenoid’s fraction. Results: The preliminary phytochemical analysis revealed that the terpenoids obtained from the leaf had the highest total phenol and flavonoid content. Both leaf and rhizome extracts had modest antioxidant capacities compared to ascorbic acid. Similarly, the rhizome extract had significantly higher α-amylase inhibitory activity than the standard acarbose (P < 0.05). Overall, the rhizome extract of K. pulchra outperformed the leaf extract in terms of antioxidant and antidiabetic potential. Conclusion: Kaempferia pulchra is a natural source of terpenoids with several therapeutic qualities, especially for managing diabetes. However, further research is needed to validate some of the claims ascribed to this plant.

Cellulitis is a skin disease which is caused by the pathogen Staphylococcus aureus. The protein Dihydrofolate reductase is an important drug based target for Trimethoprim. Dihydrofolate reductase is reversibly inhibited by the drug trimethoprim. One of the major enzymes responsible for converting dihydrofolic acid into tetrahydrofolic acid. The screening of microbial natural products, Soil isolation is a rich source of actinomycetes. The actinomycetes sp. KK21-2 strain isolated from the soil, the aqueous extract of the isolates yielded 33 active metabolites from the LC-MS analysis. Based on the ADMETox analysis, 8 compounds were excluded and finally 25 compounds were selected for the docking analysis. Protein structure of S. aureus retrieved from PDB, drug and compounds were obtained from the PubChem Database. Molecular docking (XP) and Induced Fit Docking (IFD) were completed utilizing the Glide Module of Schrodinger. Molecular dynamics simulation employing the Schrodinger Desmond module was used to examine the stability of the docked complex. From the results of docking analysis and MD Simulation result 5-(3,4-Dihydroxy-1,5-cyclohexadien-1-yl) phenylhydantoin have suggest this compound have multi- targeting potency against the cellulitis skin disease from the pathogen Staphylococcus aureus, in future need to be verified through in vitro and in vivo analysis.

To stay in sync with environmental cues, the body's metabolic activities must be rhythmic, and these rhythmic functions are known as circadian rhythms, which repeat every 24 h. People's sleep–wake and eating patterns were interrupted as a result of house confinement, making them more vulnerable to noncommunicable chronic diseases during the coronavirus disease 2019 (COVID-19) period. During the epidemic, there was a greater degree of misalignment with this synchronization. The effects of severe acute respiratory syndrome coronavirus 2 (COVID-19) on the human circadian clock are studied in depth. The literature review was conducted fully online, with the website utilized to collect all of the papers from PubMed, and duplicates were handled only in the first phase. Researchers found that individuals of all ages who are pushed to adjust their daily routines shift to the later chronotype, resulting in lifestyle modifications and an altered biological timing system that contributes to noncommunicable chronic illnesses. Chronic illnesses have bidirectional conductance, which means they can be caused by both environmental and self-modification in daily activity, as was the case during the COVID-19 outbreak, which forced people to stay at home. This review comes to the conclusion that fighting the pandemic may be best done by changing medications and focusing on immune health.

The present study validated the green synthesis and characterization of AgNPs using the leaf extracts of Solanum melongena (SM-AgNPs). The efficiency of the phytocompounds in the leaf extract in reducing Ag+ to Ag0 ions was studied. The synthesized SM-AgNPs were characterized by spectroscopic methods and standard methods were adopted for the antioxidant activity, HPTLC, cytotoxicity and antimicrobial assays and pesticidal activity as well. The synthesised nanoparticle was measured to be 20.54 nm which was confirmed by the characterization. The total phenolic content was 5.98 ± 0. GAE/g which indicated potent antioxidant activity. HPTLC profile showed thirteen peaks corresponding to the phenolic compounds with Rf values ranging from 0.05 to 0.95. The average particle size and zeta potential was 75.14 nm-19.8 mV respectively. Cytotoxicity assay of the synthesized AgNP’s showed viability percentage of 50.23 at 100 mg/mL. Treatment of MDA-MB-231 cell lines with the SM-AgNPs induced early apoptosis in a greater number of cells. The synthesizedAgNPs were effective against the bacterial isolates Escherichia coli, Pseudomonas aeruginosa, Shigella flexneri, Proteus vulgaris, Klebsiella pneumoniae and Staphylococcus aureus. High mortality of Bemisiatabaci was observed in the100 µg/mL of the SM-AgNPs treated concentrations. The study demonstrated the efficiency of SM-AgNPs against cancer cell lines, microbial isolates and insect pests.

The tyrosine kinase family receptor of discoidin domain receptors (DDR1 and DDR2) is known to be activated by extracellular matrix collagen catalytic binding protein receptors. They play a remarkable role in cell proliferation, differentiation, migration, and cell survival. DDR1 of the DDR family regulates matrix-metalloproteinase, which causes extracellular matrix (ECM) remodeling and reconstruction during unbalanced homeostasis. Collagenous-rich DDR1 triggers the ECM of cartilage to regenerate the cartilage tissue in osteoarthritis (OA) and temporomandibular disorder (TMD). Moreover, DDR2 is prominently present in the fibroblasts, smooth muscle cells, myofibroblasts, and chondrocytes. It is crucial in generating and breaking collagen vital cellular activities like proliferation, differentiation, and adhesion mechanisms. However, the deficiency of DDR1 rather than DDR2 was detrimental in cases of OA and TMDs. DDR1 stimulated the ECM cartilage and improved bone regeneration. Based on the above information, we made an effort to outline the advancement of the utmost promising DDR1 and DDR2 regulation in bone and cartilage, also summarizing their structural, biological activity, and selectivity.

Background: Methods like the bio-synthesis of silver nanoparticles (Ag NPs) using plant extracts have become promising due to their eco-friendly approach. The study aimed to examine the utilization of Garcinia gummi-gutta fruit phytochemicals as agents in the biosynthesis of Ag NPs, evaluation of the antimicrobial, antioxidant, and anti-cancerous properties, as well as the photocatalytic ability of bio-synthesized Ag NPs against Crystal Violet (CV), a triphenylmethane dye. Methods: The characterization of the physical properties of the Ag NPs synthesized via the green route was done using UV–Vis spectrophotometry (UV–Vis), X-ray Diffraction (XRD), Fourier Transform Infrared Spectrophotometry (FTIR), Scanning Electron Microscopy (SEM), Zeta potential analysis, and Transmission Electron Microscopy (TEM). The dye degradation efficiency of CV was determined using synthesized Ag NPs under UV light by analyzing the absorption maximum at 579 nm. The antimicrobial efficacy of Ag NPs against E. coli, S. aureus, Candida tropicalis, and Candida albicans was examined using the broth dilution method. The antioxidant and anti-cancer properties of the synthesized Ag NPs were assessed using the DPPH and MTT assays. Results: The UV analysis revealed that the peak of synthesized Ag NPs was 442 nm. Data from FTIR, XRD, Zeta potential, SEM, and TEM analysis confirmed the formation of nanoparticles. The SEM and TEM analysis identified the presence of spherical nanoparticles with an average size of 29.12 nm and 24.18 nm, respectively. Maximum dye degradation efficiency of CV was observed at 90.08% after 320 min without any silver leaching, confirming the photocatalytic activity of Ag NPs. The bio-efficiency of the treatment was assessed using the Allium cepa root growth inhibition test, toxicity analysis on Vigna radiata, and Brine shrimp lethality assay. Conclusions: The findings revealed the environmentally friendly nature of green Ag NPs over physical/chemically synthesized Ag NPs. The synthesized Ag NPs can effectively be used in biomedical and photocatalytic applications.

Abstract Since vitamin D's discovery, strenuous efforts to investigate its physiological exploit and deficiency on human health were done. Our body synthesizes fat‐soluble vitamin D when get exposed to sunlight. In recent years, experimental data indicate that sunlight exposure and an adequate level of circulating vitamin D can reduce the incidence of cancer. Several in vitro and in vivo studies also suggest vitamin D as a potentially valuable supplement for cancer treatment and prevention. Nevertheless, there need to be adequate clinical studies performed to substantiate the suppressive ability of vitamin D concerning cancer incidence. Thus, understanding the cellular mechanisms of vitamin D can be advantageous for preventing several chronic diseases. Consequently, this review concentrates on different studies that have been conducted to characterize the outcome of vitamin D in reducing cancer incidence and its medication by cellular progression mechanism.

Being considered minor vexations, fungal infections hinder the life of about 15% of the world population superficially, with rare threats to life in case of invasive sepsis. A significant rise in the intrusive mycoses due to machiavellian fungal species is observed over the years due to increased pathology and fatality in people battling life-threatening diseases. Individuals undergoing therapy with immune suppressive drugs plus recovering from viral infections have shown to develop fungal sepsis as secondary infections while recovering or after. Currently, the whole world is fighting against the fright of Coronavirus disease (COVID-19), and corticosteroids being the primitive therapeutic to combat the COVID-19 inflammation, leads to an immune-compromised state, thereby allowing the not so harmful fungi to violate the immune barrier and flourish in the host. A wide range of fungal co-infection is observed in the survivors and patients of COVID-19. Fungal species of Candida, Aspergillus and Mucorales, are burdening the lives of COVID-19 patients/survivors in the form of Yellow/Green, White and Black fungus. This is the first article of its kind to assemble note on fungal infections seen in the current human health scenario till date and provides a strong message to the clinicians, researchers and physicians around the world “non-pathological fungus should not be dismissed as contaminants, they can quell immunocompromised hosts”.

Diabetes mellitus (DM) is a collection of metabolic and pathophysiological disorders manifested with high glucose levels in the blood due to the inability of β-pancreatic cells to secrete an adequate amount of insulin or insensitivity of insulin towards receptor to oxidize blood glucose. Nevertheless, the preceding definition is only applicable to people who do not have inherited or metabolic disorders. Suppose a person who has been diagnosed with Type 1 or Type 2DM sustains an injury and the treatment of the damage is complicated and prolonged. In that case, the injury is referred to as a diabetic foot ulcer (DFU). In the presence of many proliferating macrophages in the injury site for an extended period causes the damage to worsen and become a diabetic wound. In this review, the scientific information and therapeutic management of DM/DFU with nanomedicine, and other related data were collected (Web of Science and PubMed) from January 2000 to January 2022. Most of the articles revealed that standard drugs are usually prescribed along with hypoglycaemic medications. Conversely, such drugs stabilize the glucose transporters and homeostasis for a limited period, resulting in side effects such as kidney damage/failure, absorption/gastrointestinal problems, and hypoglycemic issues. In this paper, we review the current basic and clinical evidence about the potential of medicinal plants, gene therapy, chemical/green synthesized nanoparticles to improving the metabolic profile, and facilitating the DM and DFU associated complications. Preclinical studies also reported lower plasma glucose with molecular targets in DM and DFU. Research is underway to explore chemical/green synthesized nanoparticle-based medications to avoid such side effects. Hence, the present review is intended to address the current challenges, recently recognized factors responsible for DM and DFU, their pathophysiology, insulin receptors associated with DM, medications in trend, and related complications.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus can cause a sudden respiratory disease spreading with a high mortality rate arising with unknown mechanisms. Still, there is no proper treatment available to overcome the disease, which urges the research community and pharmaceutical industries to screen a novel therapeutic intervention to combat the current pandemic. This current study exploits the natural phytochemicals obtained from clove, a traditional natural therapeutic that comprises important bioactive compounds used for targeting the main protease of SARS-CoV-2. As a result, inhibition of viral replication effectively procures by targeting the main protease, which is responsible for the viral replication inside the host. Pharmacokinetic studies were evaluated for the property of drug likeliness. A total of 53 bioactives were subjected to the study, and four among them, namely, eugenie, syzyginin B, eugenol, and casuarictin, showed potential binding properties against the target SARS-CoV-2 main protease. The resultant best bioactive was compared with the commercially available standard drugs. Furthermore, validation of respective compounds with a comprehensive molecular dynamics simulation was performed using Schrödinger software. To further validate the bioactive phytochemicals and delimit the screening process of potential drugs against coronavirus disease 2019, in vitro and in vivo clinical studies are needed to prove their efficacy.

The main objective of the present research work is to assess the biological properties of the aqueous plant extract (ACAE) synthesised silver nanoparticles from the herbal plant Ageratum conyzoides, and their biological applications. The silver nanoparticle syntheses from Ageratum conyzoides (Ac-AgNPs) were optimised with different parameters, such as pH (2, 4, 6, 8 and 10) and varied silver nitrate concentration (1 mM and 5 mM). Based on the UV–vis spectroscopy analysis of the synthesised silver nanoparticles, the concentration of 5 mM with the pH at 8 was recorded as the peak reduction at 400 nm; and these conditions were optimized were used for further studies. The results of the FE-SEM analysis recorded the size ranges (~30–90 nm), and irregular spherical and triangular shapes of the AC-AgNPs were captured. The characterization reports of the HR-TEM investigation of AC-AgNPs were also in line with the FE-SEM studies. The antibacterial efficacies of AC-AgNPs have revealed the maximum zone of inhibition against S. typhi to be within 20 mm. The in vitro antiplasmodial activity of AC-AgNPs is shown to have an effective antiplasmodial property (IC50:17.65 μg/mL), whereas AgNO3 has shown a minimum level of IC50: value 68.03 μg/mL, and the Ac-AE showed >100 μg/mL at 24 h of parasitaemia suppression. The α-amylase inhibitory properties of AC-AgNPs have revealed a maximum inhibition similar to the control Acarbose (IC50: 10.87 μg/mL). The antioxidant activity of the AC-AgNPs have revealed a better property (87.86% ± 0.56, 85.95% ± 1.02 and 90.11 ± 0.29%) when compared with the Ac-AE and standard in all the three different tests, such as DPPH, FRAP and H2O2 scavenging assay, respectively. The current research work might be a baseline for the future drug expansion process in the area of nano-drug design, and its applications also has a lot of economic viability and is a safer method in synthesising or producing silver nanoparticles.

Advancements and developments in the 3D bioprinting have been promising and have met the needs of organ transplantation. Current improvements in tissue engineering constructs have enhanced their applications in regenerative medicines and other medical fields. The synergistic effects of 3D bioprinting have brought technologies such as tissue engineering, microfluidics, integrated tissue organ printing, in vivo bioprinted tissue implants, artificial intelligence and machine learning approaches together. These have greatly impacted interventions in medical fields, such as medical implants, multi-organ-on-chip models, prosthetics, drug testing tissue constructs and much more. This technological leap has offered promising personalized solutions for patients with chronic diseases, and neurodegenerative disorders, and who have been in severe accidents. This review discussed the various standing printing methods, such as inkjet, extrusion, laser-assisted, digital light processing, and stereolithographic 3D bioprinter models, adopted for tissue constructs. Additionally, the properties of natural, synthetic, cell-laden, dECM-based, short peptides, nanocomposite and bioactive bioinks are briefly discussed. Sequels of several tissue-laden constructs such as skin, bone and cartilage, liver, kidney, smooth muscles, cardiac and neural tissues are briefly analyzed. Challenges, future perspectives and the impact of microfluidics in resolving the limitations in the field, along with 3D bioprinting, are discussed. Certainly, a technology gap still exists in the scaling up, industrialization and commercialization of this technology for the benefit of stakeholders.

The Asteraceae family is one of the largest families in the plant kingdom with many of them extensively used for significant traditional and medicinal values. Being a rich source of various phytochemicals, they have found numerous applications in various biological fields and have been extensively used for therapeutic purposes. Owing to its potential phytochemicals present and biological activity, these plants have found their way into pharmaceutical industry as well as in various aspects of nanotechnology such as green synthesis of metal oxide nanoparticles. The nanoparticles developed from the plants of Asteraceae family are highly stable, less expensive, non-toxic, and eco-friendly. Synthesized Asteraceae-mediated nanoparticles have extensive applications in antibacterial, antifungal, antioxidant, anticancer, antidiabetic, and photocatalytic degradation activities. This current review provides an opportunity to understand the recent trend to design and develop strategies for advanced nanoparticles through green synthesis. Here, the review discussed about the plant parts, extraction methods, synthesis, solvents utilized, phytochemicals involved optimization conditions, characterization techniques, and toxicity of nanoparticles using species of Asteraceae and their potential applications for human welfare. Constraints and future prospects for green synthesis of nanoparticles from members of the Asteraceae family are summarized.

An emerging field of science “Nanotechnology” which is involved in manipulation of atoms and molecules has shown great potential in all fields of sciences. Nanotechnology deals with nanoparticles ranging from size 1 to 100 nm in diameter, due to small size and high surface area eventually increases the state of activity. This review focuses on metal and metal oxide nanoparticles and mainly on green synthesis, characterization and application of copper nanoparticles. Green synthesis of copper and copper oxide (Cu and CuO) is economically beneficial and ecofriendly. Copper nanoparticles are used in diverse fields such as biomedicine, pharmaceuticals, bioremediation, molecular biology, bioengineering, genetic engineering, dye degradation, catalysis, cosmetics and textiles. Structural properties and biological effects of copper nanoparticles have promising effectivity in field of life sciences.

Newly emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causing coronavirus disease 2019 (COVID-19) pandemic has now spread across the globe in past few months while affecting 26 million people and leading to more than 0.85 million deaths as on 2nd September, 2020. Severity of SARS-CoV-2 infection increases in COVID-19 patients due to pre-existing health co-morbidities. This mini-review has focused on the three significant co-morbidities viz., heart disease, hypertension, and diabetes, which are posing high health concerns and increased mortality during this ongoing pandemic. The observed co-morbidities have been found to be associated with the increasing risk factors for SARS-CoV-2 infection and COVID-19 critical illness as well as to be associated positively with the worsening of the health condition of COVID-19 suffering individuals resulting in the high risk for mortality. SARS-CoV-2 enters host cell via angiotensin-converting enzyme 2 receptors. Regulation of crucial cardiovascular functions and metabolisms like blood pressure and sugar levels are being carried out by ACE2. This might be one of the reasons that contribute to the higher mortality in COVID-19 patients having co-morbidities. Clinical investigations have identified higher levels of creatinine, cardiac troponin I, alanine aminotransferase, NT-proBNP, creatine kinase, D-dimer, aspartate aminotransferase and lactate dehydrogenase in patients who have succumbed to death from COVID-19 as compared to recovered individuals. More investigations are required to identify the modes behind increased mortality in COVID-19 patients having co-morbidities of heart disease, hypertension, and diabetes. This will enable us to design and develop suitable therapeutic strategies for reducing the mortality. More attention and critical care need to be paid to such high risk patients suffering from co-morbidities during COVID-19 pandemic.

This study extensively investigated to find out the in-vivo acute toxicity and lethal concentration (LC50) of biosynthesized Ag NPs in Labeo rohita (L. rohita). The haematological studies and antioxidative responses were considered in three tissues such as gill, liver and muscle of L. rohita. The results of this study showed that increasing the dose of Ag NPs led to bioaccumulation in the tissues. The haematological analysis showed considerable alterations in the Ag NPs-treated fish. The impact of histological changes induced by Ag NPs were confirmed by the damages in the tissues, primary lamella, blood vessels and formation of vacuolation in liver and muscle when compared with the control L. rohita.

Abstract Angiotensin converting enzyme 2 (ACE2) and main protease (MPro) are significant target proteins, mainly involved in the attachment of viral genome to host cells and aid in replication of severe acute respiratory syndrome‐coronaviruses or SARS‐CoV genome. In the present study, we identified 11 potent bioactive compounds from ethanolic leaf extract of Ipomoea obscura (L.) by using GC‐MS analysis. These potential bioactive compounds were considered for molecular docking studies against ACE2 and MPro target proteins to determine the antiviral effects against SARS‐COV. Results exhibits that among 11 compounds from I. obscura (L.), urso‐deoxycholic acid, demeclocycline, tetracycline, chlorotetracycline, and ethyl iso‐allocholate had potential viral inhibitory activity. Hence, the present findings suggested that chemical constitution present in I. obscura (L.) will address inhibition of corona viral replication in host cells.

Autism and autism spectrum disorders (ASD) affects the areas of social awareness and interaction, verbal and nonverbal communication, and behaviors and interests, which are grouped among neurobehavioral disorders. One in 68 children is affected with ASD according to the estimate from the Center for Disease Control and Prevention's autism and developmental disabilities monitoring network and an increasing prevalence observed worldwide. A characteristic heterogeneity in ASD determined genetic variability as a major contributor. Whole-exome sequencing of ASD individuals revealed that discrete de novo mutation (single-nucleotide variation or small indels) also contribute to the overall genetic risk of ASD apart from rare genetic variation affecting single nucleotides of protein-coding DNA or rare genomic copy number variants as assessed through other high-throughput genomic methods. These genes are involved in synaptic transmission and regulated during brain development by acting upstream or downstream of Wingless- related integration site (WNT), bone morphogenetic proteins/transforming growth factor-β, sonic hedgehog, fibroblast growth factor, and retinoic acid signaling pathways. The current review focuses on genes involved in synaptic function, undergoing de novo mutation leading to ASD condition.

Alzheimer's disease is a degenerative brain disease and the most common cause of dementia. The characteristic symptoms of dementia are difficulties with memory, language, problem solving, and other cognitive skills which affects a person's ability to perform everyday activities. Trace elements have found to play a major role in the Alzheimer's disease. Nowadays, the association of trace elements in the pathogenesis of Alzheimer's disease is more considered, and trace elements such as aluminum (Al), iron (Fe), copper (Cu), and zinc (Zn) imbalances in the amyloid beta peptides have been seen in the Alzheimer's disease This review focuses on the accumulation of trace elements aluminum (Al), copper (Cu), iron (Fe), and zinc (Zn) in the pathogenesis of Alzheimer's disease.

The tracing of an alternative drug, Phytochemicals is a promising approach to the viral threats that have emerged over the past two years. Across the world, herbal medicine is a better solution against anti-viral diseases during pandemic periods. Goniothalamus wightii is an herbal plant, which has diverse bioactive compounds with anticancer, antioxidant, and anti-viral properties. The aim of the study was to isolate the compound by chromatography studies and functionalization by FT-IR, LC-MS, and NMR (C-NMR, H-NMR). As a result, the current work focuses on whether (S)-Goniathalamin and its analogue could act as natural anti-viral molecules for multiple target proteins viz., MPro, RdRp, and SPro, which are required for SARS-CoV-2 infection. Overall, 954 compounds were examined and the molecular-docking studies were performed on the maestro platform of Schrodinger software. Molecular-dynamics simulation studies were performed on two complex major compounds to confirm their affinity across 150 simulations. This research suggests that plant-based drugs have high levels of antiviral properties against coronavirus. However, more research is needed to verify its antiviral properties.

In the present study, the ethanolic leaf extract of Azima tetracantha Lam (A. tetracantha) was investigated for antipyretic activity in rats using Brewer’s yeast induced Pyrexia. The leaves of A. tetracantha were collected from Ponnamaravathi and cut into small pieces and shade dried. The dried powdered leaves (100 gm) were extracted in a Soxhlet apparatus by using 95% ethanol. Albino rats weighing (200-250g) were taken for the experiment divided into four groups of six animals each. Group 1 received 3% aqueous suspension of gum Acacia (1 ml/200g) as vehicle orally, group 2 and group 3 received ethanolic leaf extract of A. tetracantha 100 and 200 mg/kg with 3% aqueous suspension of gum Acacia orally and the group 4 served as standard received paracetamol 25 mg/kg with 3% aqueous suspension of gum Acacia orally. The subcutaneous injection of yeast suspension markedly elevated the rectal temperature after 18h of administration. Treatment with A. tetracantha extract at a dose of 100, 200 mg/kg decreased the rectal temperature of the rats in dose dependent manner. This effect was maximal at dose of 200 mg/kg and it caused significant lowering of body temperature (P< 0.01) up to 4 hour after its administration. The antipyretic effect started as early as 1h and the effect was maintained for 4h, after its administration. Both the standard drug paracetamol 25 mg/kg and tested drug A. tetracantha extract were significantly reduced the yeast elevated rectal temperature, at 2nd, 3rd and 4th hour compared to control group.

Cardiovascular disease, in particular coronary artery disease (CAD), is the principal cause of mortality in developed countries. The classical acute phase protein, C-reactive protein (CRP) is an exquisitely sensitive systemic marker of disease with broad clinical utility for monitoring and differential diagnosis. In recent years, acute phase reactants have been shown to predict future cardiovascular events in individuals with and without established CAD. Atorvastatin, a 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, substantially reduce cardiovascular morbidity and mortality, and recently their anti-inflammatory properties have been investigated. The present study was therefore designed to determine the effects of atorvastatin on CRP in patients with CAD. Ninety two patients with or without or at the risk of CAD were recruited for the study, of which 35 belongs to control (untreated) and 57 were test group, in which, 30 of them received daily with 20 mg/day of atorvastatin and the remaining 27 were untreated. The patients were followed for over a period of 6 weeks. For entire study population, CRP along with lipid profile, SGOT, SGPT, urea and creatinine were measured 1st day and at the end of 6th week of the treatment. For patients with or at risk of CAD, the reduced rate of progression of atherosclerosis associated with intensive atorvastatin treatment, as compared with control is significantly related to greater reduction in the levels of both atherogenic lipoproteins and CRP. This may be important with respect to the early benefits of atorvastatin therapy.

Boerhavia diffusa L. Nyctanginaceae (B. diffusa) is a medicinal herb commonly considered as a weed. The exploration of phytochemicals in different parts of B. diffusa with different solvents will create awareness, along with the suitable solvent and method for extraction of pharmaceutical compounds. Hence, the present study focuses on phytochemical analysis of B. diffusa leaves, stems, and roots in various solvents with hot and cold extraction. The decoctions performed well in most of the qualitative and quantitative tests, along with the DPPH assay. The aqueous extract showed a good result in the FRAP assay and ABTS assay. In the antimicrobial test, the B. diffusa root ethanol extract inhibited the growth of Pseudomonas aeruginosa and Staphylococcus aureus with zones of inhibition of about 8 mm and 20 mm at 200 µg concentration, respectively. Using a molecular docking approach, the top four ranked molecules from the crude extract of B. diffusa profiled from GC–MS spectroscopy in terms of growth inhibition of the pathogenic bacterium P. aeruginosa were selected; among them, 2-(1,2 dihydroxyethyl)-5-[[2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-3,4-dihydrochromen-6-yl]oxy]oxolane-3,4-diol exhibited the minimum binding score, revealing high affinity in complex. B. diffusa is highly nutritious, and the maceration and decoction extracts were similar except for the chloroform extract that was found to be weak.