33 results on '"Vunduk, Jovana"'
Search Results
2. Bioprospecting of Selected Species of Polypore Fungi from the Western Balkans
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Kozarski, Maja, primary, Klaus, Anita, additional, Špirović-Trifunović, Bojana, additional, Miletić, Srdjan, additional, Lazić, Vesna, additional, Žižak, Željko, additional, and Vunduk, Jovana, additional
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- 2024
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3. Effect of modified atmosphere packaging on selected functional characteristics of Agaricus bisporus
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Vunduk, Jovana, Kozarski, Maja, Djekic, Ilija, Tomašević, Igor, and Klaus, Anita
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- 2021
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4. Impact of grape pomace as a cultivation substrate on the Pleurotus ostreatus chemical and biological properties
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Doroški Ana, Klaus Anita, Kozarski Maja, Nikolić Biljana, Vunduk Jovana, Lazić Vesna, and Đekić Ilija
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pleurotus ostreatus ,food waste ,quality index ,grape pomace ,mushroom ,Technology (General) ,T1-995 - Abstract
The objective of this study was to develop a single quality index of chemical characteristics of Pleurotus ostreatus extracts on 7th and 14th day of its shelf life, derived from the mushroom fruiting bodies. P. ostreatus was cultivated on four substrates containing different ratio of wine industry waste-grape pomace (P) and wheat straw (S): 100P, 80P20S, 50P50S, 20P80S. Four quality parameters of P. ostreatus mushroom extracts, i.e. antioxidative parameters: ABTS+ and DPPH• free radical scavenging capability, total phenolic compounds (TPC) and total polysaccharides (TPS) were used to define the final extract quality index. Analysis indicated 100P and 80P20S as the samples cultivated on the substrate with higher percent of grape pomace, as the best quality at the 7th day of its shelf life. On the other hand, final quality score indicated 50P50S and 20P80S, cultivated on a substrate with a lower percent of grape pomace, as the best quality samples at the 14th day of its shelf life. According to the results, samples cultivated on a higher pomace content substrate are of better quality in a shorter storage time period.
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- 2021
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5. Improvement of Alginate Extraction from Brown Seaweed (Laminaria digitata L.) and Valorization of Its Remaining Ethanolic Fraction.
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Savić Gajić, Ivana M., Savić, Ivan M., Ivanovska, Aleksandra M., Vunduk, Jovana D., Mihalj, Ivana S., and Svirčev, Zorica B.
- Abstract
This study aimed to improve the conventional procedure of alginate isolation from the brown seaweed (Laminaria digitata L.) biomass and investigate the possibility of further valorization of the ethanolic fraction representing the byproduct after the degreasing and depigmentation of biomass. The acid treatment of biomass supported by ultrasound was modeled and optimized regarding the alginate yield using a response surface methodology based on the Box–Behnken design. A treatment time of 30 min, a liquid-to-solid ratio of 30 mL/g, and a treatment temperature of 47 °C were proposed as optimal conditions under which the alginate yield related to the mass of dry biomass was 30.9%. The use of ultrasonic radiation significantly reduced the time required for the acid treatment of biomass by about 4 to 24 times compared to other available conventional procedures. The isolated alginate had an M/G ratio of 1.08, which indicates a greater presence of M-blocks in its structure and the possibility of forming a soft and elastic hydrogel with its use. The chemical composition of the ethanolic fraction including total antioxidant content (293 mg gallic acid equivalent/g dry weight), total flavonoid content (14.9 mg rutin equivalent/g dry weight), contents of macroelements (the highest content of sodium, 106.59 mg/g dry weight), and microelement content (the highest content of boron, 198.84 mg/g dry weight) was determined, and the identification of bioactive compounds was carried out. The results of ultra high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry analysis confirmed the presence of 48 compounds, of which 41 compounds were identified as sugar alcohol, phenolic compounds, and lipids. According to the 2,2-diphenyl-1-picrylhydrazyl assay, the radical scavenging activity of the ethanolic fraction (the half-maximal inhibitory concentration of 42.84 ± 0.81 μg/mL) indicated its strong activity, which was almost the same as in the case of the positive control, synthetic antioxidant butylhydroxytoluene (the half-maximal inhibitory concentration of 36.61 ± 0.79 μg/mL). Gram-positive bacteria (Staphylococcus aureus, Enterococcus faecalis, and Bacillus cereus) were more sensitive to the ethanolic fraction compared to Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, and Shigella sonnei). The obtained results indicated the possibility of the further use of the ethanolic fraction as a fertilizer for plant growth in different species and antifouling agents, applicable in aquaculture. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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6. The influence of mushroom Coriolus versicolor and hazelnuts enrichment on antioxidant activities and bioactive content of dark chocolate
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Kozarski Maja S., Klaus Anita S., Vunduk Jovana Đ., and Nikšić Miomir P.
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antioxidant potency composite index ,dark chocolate ,enriched dark chocolate products ,Food processing and manufacture ,TP368-456 - Abstract
Oxidative stress is among the main culprits for the progression of chronic diseases such as cardiovascular diseases, diabetes, and neurodegenerative disorders. It is well known that dark chocolate possesses polyphenols as major constituents which dietary consumption has been associated to health beneficial effects. Consequently, this study aimed to analyze dark chocolate (DC) and dark chocolate enriched with medicinal mushroom Coriolus versicolor (DCC) and hazelnuts (DCH) regarding antioxidant potential and total polyphenol (TPC), flavonoid (TFC), flavan-3-ol (FLA) and proanthocyanidin (PCA) content. DPPH•, CUPRAC and ABTS•+ assays were applied for measuring antioxidant capacity. The average of all antioxidant tests for each product was used for calculating the antioxidant potency composite index (ACI). The mean values of all antioxidant assays indicated that all chocolate products contained potent antioxidants. The addition of hazelnuts to dark chocolate significantly affected its total flavonoid content. Regression analysis among results obtained with antioxidant assays revealed that dark chocolate and dark chocolate products may act in mixed mode, by direct reduction via electron transfer or by radical quenching via H atom transfer. Flavan-3ols had the most significant impact on the ability of the analyzed samples to reduce metals, while proanthocyanidins primarily acted as radical scavengers. The obtained results provided additional information regarding the value-added dark chocolates enriched with bioactive compounds of medicinal mushroom and plant origin.
- Published
- 2020
7. Health impact of the commercially cultivated mushroom Agaricus bisporus and wild-growing mushroom Ganoderma resinaceum - a comparative overview
- Author
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Kozarski Maja S., Klaus Anita S., Vunduk Jovana Đ., Jakovljević Dragica M., Jadranin Milka B., and Nikšić Miomir P.
- Subjects
hot water extracts ,cytotoxicity ,enzyme inhibition ,lipid peroxidation ,Chemistry ,QD1-999 - Abstract
The health promoting effects of hot water extracts obtained from fruiting bodies of the commercially cultivated mushroom Agaricus bisporus (AbHW) and the wild-growing mushroom Ganoderma resinaceum (GrHW) originating from northern Serbia are presented in this research. These abilities were compared in vitro by the prevention of lipid peroxidation (LPx) in a linoleic acid model system, inhibition of the angiotension converting I enzyme (ACE) that could help in the maintenance of a normal blood pressure level and strengthening the ability of the central cholinergic neuron by inhibiting the activity of acetylcholinesterase (AChE). Cytotoxic activities were observed towards selected human malignant (HeLa and K562) cell lines and normal- -human peripheral blood mononuclear cells (PBMC). GrHW contains higher phenolics (5.9 g (100 g)-1), inhibition of LPx (EC50 = 1.07 mg mL-1), ACE (IC50 = 0.54 mg mL-1) and AChE (IC50 = 0.37 mg mL-1), and exhibited a significant selectivity in the antitumour action against HeLa (IC50 = 0.14 mg mL-1) and K562 (IC50 = 0.11 mg mL-1) cells. AbHW contained higher total protein (6.4 g (100 g)-1), carbohydrate (75.4 g (100 g)-1) and β-glucan (55.1 g (100 g)-1) contents and induced significant proliferation of healthy PBMC from 152–116 % in the concentration range of 0.047–0.187 mg mL-1. The difference in the biological activity of the extracts provides guidance on their use as functional food. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III 46001, Grant no. III 46010 and Grant no. III 43004]
- Published
- 2020
- Full Text
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8. The Effect of Green Extraction Technologies on the Chemical Composition of Medicinal Chaga Mushroom Extracts.
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Lazić, Vesna, Klaus, Anita, Kozarski, Maja, Doroški, Ana, Tosti, Tomislav, Simić, Siniša, and Vunduk, Jovana
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INDUSTRIAL chemistry ,GLUCANS ,POLYSACCHARIDES ,PHENOLS ,CHLOROGENIC acid ,EXTRACTION techniques ,MUSHROOMS ,ETHANOL - Abstract
The mushroom industry should implement green extraction technologies; however, there is not enough information on the differences between these techniques expressed as the chemical composition of the resulting extract. In this study, selected types of green extraction techniques (GETs) were used on Chaga (Inonotus obliquus) (Fr.) Pilát from Serbia (IS) and Mongolia (IM) to examine the differences that would enable the composition-based technology choices in the mushroom supplement industry. Subcritical water extraction (SWE), microwave-assisted (MW) extraction, and ultrasonic-assisted extraction (VAE) were used to prepare the extracts. SWE was performed at two different temperatures (120 and 200 °C), while 96% ethanol, 50% ethanol, and water were used for MW and VAE. The yield, the content of total phenols, total proteins, and carbohydrates, qualitative and quantitative analysis of phenolic compounds, carbohydrates, including α- and β- and total glucans, and fatty acids, were determined in the obtained extracts. SWE resulted in a significantly higher yield, total polysaccharide, and glucan content than any other technique. Glucose was the most dominant monosaccharide in the SWE samples, especially those extracted at 200 °C. The MW 50% EtOH extracts showed the highest yield of total phenols. Among the tested phenolic compounds, chlorogenic acid was the most dominant. SWE can be recommended as the most efficient method for extracting commercially important compounds, especially glucans and phenols. [ABSTRACT FROM AUTHOR]
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- 2024
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9. Immobilization of Chaga extract in alginate beads for modified release: Simplicity meets efficiency
- Author
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Petrović Predrag, Ivanović Katarina, Octrue Charly, Tumara Mića, Jovanović Aleksandra, Vunduk Jovana, Nikšić Miomir, Pjanović Rada, Bugarski Branko, and Klaus Anita
- Subjects
mushrooms ,natural product ,antioxidant ,antimicrobial ,encapsulation ,Chemical technology ,TP1-1185 - Abstract
Chaga (Inonotus obliquus) is a parasitic fungus, which has been used in traditional medicine in Russia and other northern European countries in the treatment of cancer, gastrointestinal andliver diseases. It has been a subject of intensive researchrecently, confirming many of its health-beneficial effects. In order to obtain a product that would allow modified and prolonged release of the Chaga’s active metabolites, hot water Chaga extract was immobilized using calcium - alginate. The extract, which was predominantly composed of carbohydrates (57 %), also contained a relatively high amount of antioxidants/phenolic compounds (130 mg gallic acid equivalents per g of dry extract) and exhibited pronounced radical scavenging activity. It showed significant antibacterial activity as well, inhibiting growth of tested bacterial strains at concentrations of 1.25-20 mg/mL. Entrapment efficiency was about 80 %, and the extract-alginate system showed pH-dependant extract release; there was negligible release at pH 1.75 (gastric pH), and the release gradually increased with the increase in pH, reaching ~43 % of immobilized extract at pH 8.5 after 90 min. Such a product could be used as a dietary supplement, adjuvant in therapy of gastrointestinal diseases or as a food additive. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III46010]
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- 2019
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10. Artificial Neural Network Prediction of Antiadhesion and Antibiofilm-Forming Effects of Antimicrobial Active Mushroom Extracts on Food-Borne Pathogens
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Vunduk, Jovana and Vunduk, Jovana
- Abstract
The problem of microbial biofilms has come to the fore alongside food, pharmaceutical, and healthcare industrialization. The development of new antibiofilm products has become urgent, but it includes bioprospecting and is time and money-consuming. Contemporary efforts are directed at the pursuit of effective compounds of natural origin, also known as “green” agents. Mushrooms appear to be a possible new source of antibiofilm compounds, as has been demonstrated recently. The existing modeling methods are directed toward predicting bacterial biofilm formation, not in the presence of antibiofilm materials. Moreover, the modeling is almost exclusively targeted at biofilms in healthcare, while modeling related to the food industry remains under-researched. The present study applied an Artificial Neural Network (ANN) model to analyze the anti-adhesion and anti-biofilm-forming effects of 40 extracts from 20 mushroom species against two very important food-borne bacterial species for food and food-related industries—Listeria monocytogenes and Salmonella enteritidis. The models developed in this study exhibited high prediction quality, as indicated by high r2 values during the training cycle. The best fit between the modeled and measured values was observed for the inhibition of adhesion. This study provides a valuable contribution to the field, supporting industrial settings during the initial stage of biofilm formation, when these communities are the most vulnerable, and promoting innovative and improved safety management. © 2023 by the authors.
- Published
- 2023
11. Artificial Neural Network Prediction of Antiadhesion and Antibiofilm-Forming Effects of Antimicrobial Active Mushroom Extracts on Food-Borne Pathogens
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Vunduk, Jovana, primary, Klaus, Anita, additional, Lazić, Vesna, additional, Kozarski, Maja, additional, Radić, Danka, additional, Šovljanski, Olja, additional, and Pezo, Lato, additional
- Published
- 2023
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12. Molecular Approaches for Detection of Trichoderma Green Mold Disease in Edible Mushroom Production
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Šašić Zorić, Ljiljana, primary, Janjušević, Ljiljana, additional, Djisalov, Mila, additional, Knežić, Teodora, additional, Vunduk, Jovana, additional, Milenković, Ivanka, additional, and Gadjanski, Ivana, additional
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- 2023
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13. Roles of Medicinal Mushrooms as Natural Food Dyes and Dye-Sensitised Solar Cells (DSSC): Synergy of Zero Hunger and Affordable Energy for Sustainable Development
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Ahmad, Nurfadzilah, primary, Vunduk, Jovana, additional, Klaus, Anita, additional, Dahlan, Nofri Yenita, additional, Ghosh, Soumya, additional, Muhammad-Sukki, Firdaus, additional, Dufossé, Laurent, additional, Bani, Nurul Aini, additional, and Wan-Mohtar, Wan Abd Al Qadr Imad, additional
- Published
- 2022
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14. Zeolites as possible biofortifiers in Maitake cultivation
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Vunduk Jovana, Klaus Anita, Kozarski Maja, Đorđević R., Jovanović Lj., and Nikšić M.
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Grifola frondosa ,Maitake mushroom ,biofortification ,zeolite ,Minazel Plus ,ICP-OES ,Biology (General) ,QH301-705.5 - Abstract
The levels of Ni, Cu and Mg in Grifola frondosa (also known as Maitake mushroom) fruit body produced on zeolite Minazel Plus (MG)-supplemented substrate were measured with inductively coupled plasma optical emission spectrometry (ICP-OES). Two different concentrations of MG were added to the substrate for mushroom cultivation. Levels of selected metals were measured in cultivated dry carpophores. The content of Ni increased in fruit bodies produced on supplemented substrate, while in case of Cu, a pronounced decrease was observed. When two different concentrations of MG were implemented, the Mg level showed both positive and negative trend, depending on the applied concentration of zeolite. MG in a concentration of 1% showed the strongest influence on the observed elements in the cultivated fruiting body of Maitake mushroom. [Projekat Ministarstva nauke Republike Srbije, br. 46010]
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- 2014
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15. Roles of medicinal mushrooms as natural food dyes and dye-sensitised solar cells (DSSC): synergy of zero hunger and affordable energy for sustainable development
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Ahmad, Nurfadzilah, Vunduk, Jovana, Klaus, Anita, Dahlan, Nofri Yenita, Ghosh, Soumya, Wan-Mohtar, Wan Abd Al Qadr Imad, Technological University of the Shannon: Midlands Midwest, N.A. would like to thank Universiti Teknologi MARA (UiTM) under FRGS: 600-RMC/FRGS 5/3 (119/2021), W.A.A.Q.I.W.-M. acknowledges Universiti Malaya under IIRG003A-2020IISS, and and F.M.-S. recognizes Edinburgh Napier University through the Strategic Research & Knowledge Ex change Fund (Project ID: 2848909). 'Agreement on the implementation and financing of scientific research work in 2022 between the Institute of General and Physical Chemistry in Belgrade and the Ministry of Education, Science and Technological Development of the Republic of Serbia', con tract record number: 451-03-68/2022/200051. 'Agreement on the implementation and financing of scientific research work in 2022 between the Faculty of Agriculture in Belgrade and the Ministry of Education, Science and Technological Development of the Republic of Serbia', contract record number: 451-03-68/2022-14/200116.
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Sustainability ,Food colourants ,Medicinal mushrooms ,Clean energy ,Fungal dyes ,MM [Bioscience Research Institute TUS] - Abstract
In 2015, approximately 195 countries agreed with the United Nations that by 2030, they would work to make the world a better place. There would be synergies in accomplishing the 17 Sustainable Development Goals (SDGs). Synergy using a single sustainable resource is critical to assist developing nations in achieving the SDGs as cost-effectively and efficiently possible. To use fungal dye resources, we proposed a combination of the zero hunger and affordable energy goals. Dyes are widely used in high-tech sectors, including food and energy. Natural dyes are more environment-friendly than synthetic dyes and may have medicinal benefits. Fungi are a natural source of dye that can be substituted for plants. For example, medicinal mushrooms offer a wide range of safe organic dyes that may be produced instantly, inexpensively, and in large quantities. Meanwhile, medicinal mushroom dyes may provide a less expensive choice for photovoltaic (PV) technology due to their non-toxic and environmentally friendly qualities. This agenda thoroughly explains the significance of pigments from medicinal mushrooms in culinary and solar PV applications. If executed effectively, such a large, unwieldy and ambitious agenda may lead the world towards inclusive and sustainable development. yes
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- 2022
16. Lignicolous mushroom Fomitopsis pinicola as a potent inhibitor of lipid peroxidation
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Kozarski, Maja, Klaus, Anita, Vunduk, Jovana, Lazić, Vesna, Spirović Trifunović, Bojana, Miletić, Srđan, Spasić, Snežana, and Jakovljević, Dragica
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functional food ,antioxidant potential ,lipid peroxidation ,phenol profile ,lignicolus mushroom - Abstract
Excessive production of reactive oxygen species (ROS) often results in irreversible cell damage and leads to a number of disorders such as hypertension, heart disease, diabetes, inflammation, premature aging and cancer. Considering the importance of diet in prevention of diseases associated with oxidative stress, this study was conducted to evaluate antioxidant activity of the methanol extract of wild lignicolus mushroom Fomitopsis pinicola as natural source of functional food ingredients. Nowadays functional food products based on the lignicolous mushroom species are increasingly available on the market. Their powders and extracts are consumed as dietary supplements in the form of capsules or tablets and as additives in the formulation of healthier food products.
- Published
- 2022
17. The role of Gentiana lutea extracts in reducing UV-induced DNA damage.
- Author
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Cvetković, Stefana, Vuletić, Stefana, Vunduk, Jovana, Klaus, Anita, Mitić-Ćulafić, Dragana, and Nikolić, Biljana
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GENETIC toxicology ,DNA damage ,GENTIANA ,REACTIVE oxygen species ,IRON ions ,EXTRACTS - Abstract
Ultraviolet (UV) radiation can result in DNA damage, mainly through direct formation of pyrimidine dimers and generation of reactive oxygen species, which can lead to the skin disorders including cancer. In accordance with this, the use of natural antigenotoxins and/or antioxidants could contribute to human health protection. Considering that plants are rich in both, the aim of this study was to investigate UV-protective and antioxidative properties of yellow gentian (Gentiana lutea), being well established in pharmacopeias and traditional medicine. Tested extracts were derived from root and shoot of the in vitro cultivated plants. Prescreening of the genotoxic properties of UVC, UVA, and the extracts, as well as the extracts' antigenotoxicity were estimated by applying alkaline comet assay on normal fetal lung fibroblast (MRC-5) and human melanoma cells (Hs 294T). Antioxidant potential was tested in ferrous ions chelating ferric reducing antioxidant power and cupric reducing antioxidant capacity assays. Genotoxicity testing, which revealed moderate DNA-damaging potential of root extract on MRC-5 cells and high genotoxicity of shoot extract on both cell lines, pointed out nongenotoxic concentrations that could be used in antigenotoxicity assay. Doses of 63 and 3 J/cm
2 for UVC and UVA, respectively, were established for antigenotoxicity study, since they induced sufficient DNA damage without notable cytotoxicity. Results of antigenotoxicity revealed strong protective effect of both extracts against UVC (the highest inhibitions 58% and 47%) and UVA (the highest inhibitions 69% and 60%), in Hs 294T and MRC-5 cells, respectively. Study of the antioxidative properties demonstrated stronger activity of shoot extract. Results obtained proved to be encouraging but further research of the UV-protective role of Gentiana lutea extracts and underlying molecular mechanisms is recommended. [ABSTRACT FROM AUTHOR]- Published
- 2023
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18. The role of Gentiana lutea extracts in reducing UV-induced DNA damage
- Author
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Cvetković, Stefana, primary, Vuletić, Stefana, additional, Vunduk, Jovana, additional, Klaus, Anita, additional, Mitić-Ćulafić, Dragana, additional, and Nikolić, Biljana, additional
- Published
- 2022
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19. Effects of Agaricus bisporus Mushroom Extract on Honey Bees Infected with Nosema ceranae
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Glavinic, Uros, primary, Rajkovic, Milan, additional, Vunduk, Jovana, additional, Vejnovic, Branislav, additional, Stevanovic, Jevrosima, additional, Milenkovic, Ivanka, additional, and Stanimirovic, Zoran, additional
- Published
- 2021
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20. Mushroom β-glucan and polyphenol formulations as natural immunityboosters and balancers:nature of the application
- Author
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Kozarski, Maja, Klaus, Anita, van Griensven, Leo, Jakovljevic, Dragica, Todorovic, Nina, Wan-Mohtar, Wan Abd Al Qadr Imad, and Vunduk, Jovana
- Abstract
Mushrooms are experiencing a kind of renaissance as a part of the contemporary human diet. These valuable organisms are more than food, they fit in perfectly as a novel market group known as nutra-mycoceuticals. Immune-balancing mushroom dietary fibers and secondary metabolites such as polyphenols are the main focus of the healthcare industry. Wellness and cosmetic companies are increasingly using mushroom extracts rich in these ingredients. This review considers the basic molecular immunomodulatory mechanisms of action of the most commonly used mushroom dietary fibers, β-glucans. The literature data on their bioavailability, metabolic transformations, preclinical and human clinical research, and safety are discussed. Immunomodulatory mechanisms of polyphenol ingredients are also considered. These molecules present great potential in the design of the new immunity balancerformulations according to their widespread structural diversity. Finally, we draw attention to the perspectives of modern trends in mushroom nutraceutical and cosmeceutical formulations to strengthen and balance immunity.
- Published
- 2023
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21. Компаративни прглед здравствених ефеката комерцијално узгајане гљиве Agaricus bisporus и самоникле врсте гливе Ganoderma resinaceum
- Author
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Kozarski, Maja S., Klaus, Anita, Vunduk, Jovana, Jakovljević, Dragica, Jadranin, Milka, and Nikšić, Miomir
- Subjects
cytotoxicity ,hot water extracts ,lipid peroxidation ,enzyme inhibition - Abstract
The health promoting effects of hot water extracts obtained from fruiting bodies of the commercially cultivated mushroom Agaricus bisporus (AbHW) and the wild-growing mushroom Ganoderma resinaceum (GrHW) originating from northern Serbia are presented in this research. These abilities were compared in vitro by the prevention of lipid peroxidation (LPx) in a linoleic acid model system, inhibition of the angiotension converting I enzyme (ACE) that could help in the maintenance of a normal blood pressure level and strengthening the ability of the central cholinergic neuron by inhibiting the activity of acetylcholinesterase (AChE). Cytotoxic activities were observed towards selected human malignant (HeLa and K562) cell lines and normal- -human peripheral blood mononuclear cells (PBMC). GrHW contains higher phenolics (5.9 g (100 g)-1), inhibition of LPx (EC50 = 1.07 mg mL-1), ACE (IC50 = 0.54 mg mL-1) and AChE (IC50 = 0.37 mg mL-1), and exhibited a significant selectivity in the antitumour action against HeLa (IC50 = 0.14 mg mL-1) and K562 (IC50 = 0.11 mg mL-1) cells. AbHW contained higher total protein (6.4 g (100 g)-1), carbohydrate (75.4 g (100 g)-1) and β-glucan (55.1 g (100 g)-1) contents and induced significant proliferation of healthy PBMC from 152–116 % in the concentration range of 0.047–0.187 mg mL-1. The difference in the biological activity of the extracts provides guidance on their use as functional food. У овоме раду поређен је здраствени ефекaт врелих водених екстраката добијених из плодоносних тела комерцијално узгајане јестиве гљиве Agaricus bisporus (AbHW) и самоникле врсте гљиве Ganoderma resinaceum (GrHW) из региона северне Србије. Здраствени ефекат је поређен in vitro превенцијом липидне пероксидације (LPx) у модел систему линолеинске киселине, инхибицијом ангиотензин конвертујућег ензима (ACE) који има улогу у одржавању нормалног нивоа крвног притиска и јачањем способности централних холинергичких неурона инхибицијом активности ацетилхолинестеразе (AChE). Цитотоксична активност је праћена на хуманим ћелијама тумора грлића материц (HeLa) и ћелијама хроничне мијелоидне леукемије (K562), као и на здравим мононуклеарним ћелијама периферног крвотока (PBMC). GrHW је показао већи садржај фенолних компоненти (5,9 g (100 g)-1), већу способност инхибиције LPx (EC50 = 1,07 mg mL-1), ACE (IC50 = 0,54 mg mL-1) и AChE (IC50 = 0,37 mg mL-1); показао је већу селективност у антитуморском дејству према HeLa (IC50 = 0,14 mg mL-1) и K562 (IC50 = 0,11 mg mL-1) ћелијама. AbHW је показао већи укупни садржај протеина (6,4 g (100 g)-1), угљених хидрата (75,4 g (100 g)-1) и β-глукана (55,1 g (100 g)-1) и значајно je стимулисао пролиферацију PBMC ћелија од 152–116 % у распону концентрација од 0,046–0,187 mg mL-1. Разлика у биолошкој активности екстраката даје смернице у њиховој примени као функционалнe хранe.
- Published
- 2020
22. Antioxidant Activity of Mushrooms in vitro and in Frankfurters
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Novaković, Saša, primary, Đekic, Ilija, additional, Klaus, Anita, additional, Vunduk, Jovana, additional, Đorđević, Vesna, additional, Tomovic, Vladimir, additional, Šojić, Branislav, additional, Kocić-Tanackov, Sunčica, additional, and Tomašević, Igor, additional
- Published
- 2020
- Full Text
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23. The Effect of Cantharellus Cibarius Addition on Quality Characteristics of Frankfurter during Refrigerated Storage
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Novakovic, Sasa, primary, Djekic, Ilija, additional, Klaus, Anita, additional, Vunduk, Jovana, additional, Djordjevic, Vesna, additional, Tomović, Vladimir, additional, Šojić, Branislav, additional, Kocić-Tanackov, Sunčica, additional, Lorenzo, Jose M., additional, Barba, Francisco J., additional, and Tomasevic, Igor, additional
- Published
- 2019
- Full Text
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24. Polysaccharides of Pleurotus flabellatus strain Mynuk produced by submerged fermentation as a promising novel tool against adhesion and biofilm formation of foodborne pathogens
- Author
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Vunduk, Jovana and Vunduk, Jovana
- Abstract
Foodborne bacteria biofilms present a major concern for the food industry. Although their numerous biological activities are well established, there is little research to date on the use of polysaccharides of mushroom origin as a possible solution for preventing biofilm formation. Therefore, the aim of this study was to examine the anti adhesion and antibiofilm effects of several types of Pleurotus flabellatus strain Mynuk polysaccharide extracts (PFSMpe), produced by air-lift submerged fermentation, against American Type Culture Collection (ATCC) and clinical strains of foodborne bacteria, as well as the cytotoxicity of these extracts. PFSMpe exhibited antiadhesion activity toward clinical isolates, and the percentage of adhesion inhibition was highest for water (WE) and exopolysaccharide (EXOPE) extracts (> 50%) against Enterococcus faecalis. Selected ATCC strains were more resistant than clinical strains, indicating the antiquorum sensing mechanism of PFSMpe action. Antibiofilm activity was similar to antiadhesion activity and WE showed the strongest effect, again on E. faecalis. Differences in antiadhesion and antibiofilm effects of PFSMpe may be explained by differences in chemical composition, with crude extracts showing greater efficiency due to a synergistic effect. PFSMpe did not exhibit cyctotoxic activity against normal human cell lines. Overall, the findings of this study show that PFSMpe represents a promising novel strategy against bacterial biofilms.
- Published
- 2019
25. Challenging the difference between white and brown Agaricus bisporus mushrooms: Science behind consumers choice
- Author
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Vunduk, Jovana and Vunduk, Jovana
- Abstract
Purpose The purpose of this paper is to examine compositional differences between brown and white varieties of Agaricus bisporus during shelf life and to determine if the growing demand for the brown variety is scientifically justified. Design/methodology/approach Field research enabled analyzing consumers' perceptions on intrinsic, extrinsic and quality characteristics of mushrooms. A total of 275 consumers participated in the survey. Obtained results were used for comparing white and brown varieties of A. bisporus over a period of 22 days. Mushrooms were packed in air and in modified atmosphere packaging (MAP) at 4 degrees C. Samples were analyzed for their nutritional, antioxidative and microbiological characteristics. Findings Two weeks from harvest, white variety showed higher amount of essential nutrients, while during the third week, brown mushrooms were more nutritionally valuable. Brown variety had better antioxidative ability for all 22 days of storage. Aerobic plate count (APC) was as expected for the mushrooms. MAPs significantly lowered APC in both varieties. The number of Enterobacteriaceae was equal for both varieties at the beginning, but later on they developed much faster in the case of brown variety. Field research combined with specific analyses clarified that there are no nutritive or microbiological reasons for the precedence of brown variety over white. Research limitations/implications Sensory aspect of the quality of mushrooms was not analyzed. Originality/value Market trend toward brown variety was scientifically challenged.
- Published
- 2018
26. Chemical characterization and biological properties of polysaccharide extracts of fungi Fomes fomentarius, Auricularia auricula-judae and Sparassis crispa
- Author
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Vunduk, Jovana Đ., Nikšić, Miomir, Klaus, Anita, Jakovljević, Dragica, Kozarski, Maja, and Dimitrijević-Branković, Suzana
- Subjects
polysaccharide extracts ,Fomes fomentarius ,Sparassis crispa ,enyzme modification ,polisaharidni ekstrakti ,biološka aktivnost ,Auricularia auricula-judae ,biological activity ,enzimska modifikacija - Abstract
Savremeno tržište i potrošači sve više prepoznaju sektor funkcionalne hrane. Hrana je više nego ikad energija, medicina, filozofija, trend ali i nauka. Tako više nije dovoljno tvrditi da je neka namirnica blagotvorna po zdravlje čoveka. Potrebno je identifikovati aktivne komponente, utvrditi koje biološke osobine ispoljavaju, a potom i na koji način ih je moguće izolovati, te postupak učiniti komercijalnim. Medicinske gljive predstavljaju upravo takvu namirnicu, jer sadrže brojna aktivna jedinjenja poput polisaharida, fenolnih jedinjenja, proteina, triterpena. Polisaharidi gljiva najkompleksniji su molekuli u prirodi i kao takvi modulatori imunog odgovora. Biološki odgovori polisaharida su raznovrsni što je posledica različite građe, molekulske mase, konformacije i rastvorljivosti ovih molekula. Iako mehanizmi delovanja nisu potpuno razjašnjeni uočeno je da biološki efekat polisaharida gljiva može biti umanjen zbog relativno male rastvorljivosti u vodi. Takođe, postupak ekstrakcije može se negativno odraziti na biološka svojstva ekstrakta. Cilj ove teze bilо je dobijanje polisaharidnih ekstrakata odabranih gljiva te njihova strukturna modifikacija primenom specifičnih enzima koji vrše hidrolizu α-D-glikozidnih veza. Zatim, hemijska karakterizacija ekstrakata, a potom ispitivanje bioloških osobina (antimikrobnih, antioksidativnih, citotoksičnosti i sposobnosti inhibicije angiotenzin I-konvertujućeg enzima (ACE)). Konačno, ekstrakti su upoređeni na osnovu rezultata primenjenih metoda, te su njihove hemijske osobine dovedene u vezu sa biološkim svojstvima. To je trebalo da omogući selekciju optimalnih uslova za dobijanje biološki aktivnih polisaharida gljiva, i tako obezbedi standardizacija postupka za njihovo najbolje iskorišćenje. Ekstrakcija polisaharida odabranih gljiva, Fomes fomentarius, Auricularia auricula-judae i Sparassis crispa, izvršena je postupcima vrele vodene i alkalne ekstrakcije, a sirovi vodeni ekstrakt je i naknadno prečišćen dijalizom. Sirovi polisaharidni ekstrakti modifikovani su sukcesivnim dodavanjem izoamilaze, β-amilaze, α-amilaze i pronaze. Hemijska karakterizacija ekstrakata sprovedena je primenom Megazyme glukanskog kita, spektrofotometrijskim određivanjem sadržaja ukupnih ugljenih hidrata, proteina i fenolnih jedinjenja... ector of functional food is more and more appreciated by modern market and consumers. Food is energy, medicine, philosophy, trend and science, now more than ever. Claiming that some type of food has beneficial effect on human health without proving it is past. Active components have to be identified, as well as their biological properties. Furthermore, those components have to be extracted, and the extraction procedure has to be economically justified. Medicinal mushrooms fulfill all listed characteristics, since they contain active compounds like polysaccharides, proteins, triterpene, and phenolic compounds. Polysaccharides isolated from mushrooms are marked as the most complex molecules in nature, which makes them excellent immune response modulators. Biological effects of mushrooms polysaccharides are various due to the differences in their structure, molecular mass, conformation, and water solubility. Although the mechanisms of their action are not completely clear, it was noticed that the ability to dissolve in water strongly affects mushroom’s polysaccharides biological activity. Extraction procedure can have negative impact on biological effect of these molecules, too. The aim of this thesis was to obtain polysaccharide extracts of selected mushroom species, and to modify their structure with enzymes which hydrolyze α-D-glucoside bondages. Then, to characterize extracts, and to examine their biological activity (antimicrobial, antioxidative, cytotoxic and the ability to inhibit angiotensin I-converting enzyme (ACE)). Finally, the extracts were compared, and based on the results of applied methods their chemical characteristics were connected with biological activities. This approach should enable the selection of optimal conditions for the production of biologicaly active mushroom polysaccharides. Overall, results should provide the standardized procedure for the most efficient utilization of mushroom extracts. Polysaccharides of selected mushrooms, Fomes fomentarius, Auricularia auricula-judae and Sparassis crispa, were extracted by hot water and alkali extraction, while crude water extractswere further purified by dialysis...
- Published
- 2017
27. Total quality index of Agaricus bisporus mushrooms packed in modified atmosphere
- Author
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Đekić, Ilija, Vunduk, Jovana, Tomašević, Igor, Kozarski, Maja, Petrović, Predrag, Nikšić, Miomir, and Klaus, Anita
- Subjects
modified atmosphere packaging ,total quality index ,Agaricus bisporus ,quality characteristics - Abstract
BACKGROUND: The aim of this study was to develop a total quality index and examine the effects of modified atmosphere packaging (MAP) on the quality of Agaricus bisporus mushrooms stored for 22 days at 4 ∘C. Mushrooms were packaged under three MAPs: high nitrogen packaging (HNP), low carbon dioxide packaging (LCP) and low oxygen packaging (LOP). Passive MAP with air inside initially was used as the atmosphere treatment (AIR). RESULTS: This research revealed two phases in quality deterioration of A. bisporus mushrooms. During the first week, most of the quality parameters were not statistically different. Thereafter, odor intensities were stronger for all four types of packaging. Color difference and browning index values showed significantly lower color changes for AIR and LOP compared with HNP and LCP mushrooms. CONCLUSION: The best total quality index was calculated for LOP, followed by LCP and AIR. The findings of this study are useful with respect to examining two-component MAPs, separating the limiting factors (O2 and CO2) and evaluating quality deterioration effects and the total quality index of A. bisporus mushrooms. © 2016 Society of Chemical Industry
- Published
- 2017
28. Effect of zeolite on growth of some medical industrial and mushrooms
- Author
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Nikšić, Miomir, Klaus, Anita, Vunduk, Jovana, and Savić, Milena
- Abstract
We studied the influence of natural zeolite Minazel (Z) on the dynamics of growth of mycelium and fruiting bodies of several important edible and medicinal mushrooms. Phenotypic and biological efficacy as well as the content of selenium in carpophores Grifola frondosa mushrooms. In order to more fully assessed the effect of supplementation was done and comparative testing with the addition of some mineral raw materials: apatite and bentonite, for which there are states that can induce an increase in the yield of different crops. It was found that the addition of natural zeolite from Serbia has a positive effect on all monitored parameters, and that the most effective concentration of 1%. Achieved effects can be attributed to the properties of zeolites and ion exchange capacity, sorption, buffering operation. Actual results indicate that the zeolites are very suitable as supplements in industrial production conditions mushrooms. Their use allows for faster growth and change of the functional properties of the obtained mushrooms. Ispitivan je uticaj prirodnog zeolita Minazel (Z) na dinamiku rasta micelijuma i plodonosnih tela nekoliko važnih jestivih i medicinskih gljiva. Praćena je i biološka efikasnost kao i sadržaj selena u karpoforima gljive Grifola frondosa. Da bi se potpunije ocenio efekat suplementacije, rađeno je i uporedno ispitivanje uz dodatak nekih mineralnih sirovina: apatita i bentonita, za koj postoje navodi da mogu usloviti povećanje prinosa pojedinih poljoprivrednih kultura. Utvrđeno je da dodatak prirodnog zeolita sa područja Srbije ima pozitivno delovanje na sve praćene parametre, a da je najefikasnija koncentracija 1%. Postignuti efekti mogu se pripisati osobinama zeolita: jonoizmenjivačka sposobnost, sorpcija, pufersko delovanje. Ostvareni rezultati ukazuju na to da su zeoliti veoma pogodni kao suplementi u industrijskim uslovima proizvodnje gljiva. Njihova primena omogućava brži rast i izmenu funkcionalnih osobina dobijenih pečuraka.
- Published
- 2015
29. Addition of Zeolites to Improve the Functional Characteristics of the Hen of the Wood or Maitake Medicinal Mushroom, Grifola frondosa (Agaricomycetes) '
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Vunduk, Jovana and Vunduk, Jovana
- Abstract
Kaolinite and the modified natural zeolite minazel plus (M+) were used as supplements in substrate used for the production of the medicinal mushroom Grifola frondosa. Growth stimulation, expressed as yield and biological efficiency, was observed when M+ (1%) was added. The production cycle was shortened by half as a result of the zeolites' ion-exchange ability, stimulation of enzyme activity, and water retain capacity. Inductively coupled plasma optical emission spectrometry of fruiting bodies showed the absence of heavy metals (arsenic, cadmium, and lead), whereas the concentration of calcium increased greatly and the concentrations of iron, magnesium, and zinc increased moderately under the influence of M+. Solid-state nuclear magnetic resonance showed a positive impact on the beta-glucanratio, which could have been caused by the epimerization reaction stimulated by zeolites. The functionality of the mushroom was evaluated through several antioxidant activity assays, and in all cases a positive effect was established: M+ was statistically more effective in comparison with kaolinite. A strong correlation was established between the antioxidative activity of cultivated fruiting bodies and the tested compounds (total phenolic compounds, carbohydrates, and minerals).
- Published
- 2016
30. Antibacterial and antifungal potential of wild basidiomycete mushroom Ganoderma applanatum
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Klaus, Anita, primary, Kozarski, Maja, additional, Vunduk, Jovana, additional, Petrovic, Predrag, additional, and Niksic, Miomir, additional
- Published
- 2016
- Full Text
- View/download PDF
31. Did the Iceman Know Better? Screening of the Medicinal Properties of the Birch Polypore Medicinal Mushroom, Piptoporus betulinus (Higher Basidiomycetes)
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Vunduk, Jovana and Vunduk, Jovana
- Abstract
The birch polypore Piptoporus betulinus was among two mushrooms that were found in the Iceman's bag. Recent studies indicated that P. betulinus was probably used as a religious and medicinal item. In order to examine the medicinal potential of P. betulinus, hot water (HW), partially purified (PP), and alkali extract (HA) were prepared and tested for antioxidant, antimicrobial, cytotoxic, and angiotensin I-converting enzyme (ACE) inhibitory activity. All tested samples exhibited moderate cytotoxic activity, and HW appeared as the most effective (IC50 = 0.8 +/- 0.1 mg/ml for HeLa cells). HA proved to be a good 1,1-diphenyl-2-picrylhydrazyl radical scavenger and exhibited the strongest ferric-reducing power (EC50 = 0.07 +/- 0.3 mg/ml). The same extract (HA) also expressed the strongest ferric-reducing power (EC50 = 0.99 +/- 0.1 mg/ml). Hot alkali extraction contributed significantly to ACE inhibitory activity (EC50 = 0.06 +/- 0.00 mg/ml) and to antimicrobial activity, especially against highly resistant Enterococcus faecalis (minimum inhibitory concentration: 0.156 +/- 0.000 mg/ml; and minimum bactericidal concentration: 1.25 +/- 0.00 mg/ml).
- Published
- 2015
32. Antioxidants of Edible Mushrooms
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Kozarski, Maja, primary, Klaus, Anita, additional, Jakovljevic, Dragica, additional, Todorovic, Nina, additional, Vunduk, Jovana, additional, Petrović, Predrag, additional, Niksic, Miomir, additional, Vrvic, Miroslav, additional, and van Griensven, Leo, additional
- Published
- 2015
- Full Text
- View/download PDF
33. Zeolites as possible biofortifiers in maitake cultivation
- Author
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Vunduk, Jovana and Vunduk, Jovana
- Abstract
The levels of Ni, Cu and Mg in Grifola frondosa (also known as Maitake mushroom) fruit body produced on zeolite Minazel Plus (MG)-supplemented substrate were measured with inductively coupled plasma optical emission spectrometry (ICP-OES). Two different concentrations of MG were added to the substrate for mushroom cultivation. Levels of selected metals were measured in cultivated dry carpophores. The content of Ni increased in fruit bodies produced on supplemented substrate, while in case of Cu, a pronounced decrease was observed. When two different concentrations of MG were implemented, the Mg level showed both positive and negative trend, depending on the applied concentration of zeolite. MG in a concentration of 1% showed the strongest influence on the observed elements in the cultivated fruiting body of Maitake mushroom.
- Published
- 2014
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