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Additional file 7. Apheresis Cohort 1 laboratory standard operating procedure.

Additional file 6. Supplementary Material v1.0.

Additional file 5. Apheresis Cohort 4 standard operating procedure.

Additional file 2. Apheresis Cohort 1 standard operating procedure.

Additional file 4. Apheresis Cohort 3 standard operating procedure.

Additional File 2. Supplementary methods and results for immunogenicity assessments of GAP vaccine. Text S1. Methodology for determining antibody mediated immune response to GAP vaccine. Text S2. Methodology for determining cell mediated immune response to GAP vaccine. Table S1. Antibodies used for flow cytometry. Figure S1. The gating strategy used to identify CD4+ T cells and subsets, as well as the expression of T cell activation markers. Figure S2. The anti-MSP2 IgM response over the course of the study in each subject. Figure S3. The IgG and IgM response to whole parasite lysate over the course of the study in each subject. Figure S4. The frequency of CD4+ T cells, Treg cells, Th1 cells and Tfh cells in peripheral blood mononuclear cells over the course of the study in each subject.

Additional file 1. Apheresis of subjects with induced blood stage p. vivax protocol.

Additional file 1. Additional tables.

Additional File 3. Supplementary methods and results for in vitro assessments of GAP vaccine. Text S1. Methodology for parasite genotyping. Text S2. Methodology for cytoadherence assays. Text S3. Methodology for scanning electron microscopy. Text S4. Methodology for var gene expression analysis. Table S1. Duration of in vitro culture of P. falciparum prior to harvest for RNA extraction for var gene transcription analysis. Text S5. Results of parasite genotyping. Text S6. Results of scanning electron microscopy analysis of parasite-infected erythrocytes. Text S7. Results of cytoadherence assays. Text S8. Results of var gene expression analysis. Table S2. Most abundantly expressed var genes.

Additional file 8. Apheresis Cohort 2 laboratory standard operating procedure.

Additional file 1: Table S1. Schedule of events for both studies. Table S2. Growth parameters of the 3D7-MBE-008 pilot bank (n = 2) compared to 3D7 bank using historical data (n = 177). Table S3. Growth parameters of the 3D7-V1 pilot bank (n = 2) compared to 3D7-V2 bank using historical data (n = 177). Table S4. Overall clinical score recorded for each participant during 3D7-MBE-008 study. Table S5. Overall clinical score recorded for each participant during 3D7-V1 study.

Additional file 3. Apheresis Cohort 2 standard operating procedure.

Additional File 1. Supplementary methods and results for subject eligibility and safety assessments. Text S1: Subject eligibility criteria. Table S1: Incidence of adverse events by subject. Table S2: Red blood cell alloantibody test results for Cohort 4 and phenotype of donor red blood cells used for GAP master cell bank manufacture.

Additional file 9. Apheresis Cohort 3 laboratory standard operating procedure.

This is a case of Actinomyces europaeus in the breast abscess of a penicillin-allergic woman. The mainstay of treatment for actinomycosis is penicillin, and there is a lack of literature describing nonpenicillin treatment options. A 69-year-old woman presented acutely with a breast abscess which was managed with incision and drainage and antibiotic therapy to good response. 21 days after presentation, Actinomyces were grown from the culture of pus, so the patient was recalled and more rigorous treatment and follow-up were initiated. The penicillin allergy led to difficulty in the identification of an appropriate antimicrobial agent that was also logistically feasible to be given on an outpatient IV basis. IV tigecycline followed by oral clarithromycin was found to be effective treatment.

Conscious Sedation is a pharmacological intervention which enables anxious patients to have dental treatment. Although there is a strong research tradition into\ud the efficacy of sedation modalities, there is a weak evidence base for the experience of sedation by those who use it.\ud The aim of this thesis was to explore patients’, referrers’ and providers’understandings and experiences of conscious sedation and the Secondary Care Sedation Clinics which use it.\ud A qualitative study was undertaken of stakeholders’ experiences of conscious sedation provided by five Secondary Care Sedation Clinics within the United\ud Kingdom. Data were collected through thirty one semi-structured interviews, which were transcribed verbatim and subsequently analysed using a constant comparative\ud method.\ud The data show that sedation and secondary care sedation clinics are imbued with a variety of interpretations by differing participants. Patients primarily perceive\ud sedation clinics as access points for addressing dental needs, whilst clinicians also anticipate an influential role in rehabilitating patients to primary care and recognise the importance of such settings for training future dentists. Successful sedation provision requires a variety of work, and sedation clinics play a hosting role to\ud visiting patients as hinterlands to the dental world. The outcome of patients’engagement with sedation clinics varies from breaking avoidant patterns to creating\ud a cycle of sedation dependency, whilst the process of sedation performance has a potential negative impact for providers.\ud 2\ud The purposes and processes reported by participants mirror those of Victorian\ud domestic parlours. This thesis suggests a novel theoretical construct of clinical ‘Parlours’. Such frontier social structures provide safe interaction for patients in a\ud temporarily hosting environment. They require front-stage performance augmented by back-stage work, and seek to influence patients in the long-term whilst providing\ud short-term clinical services. Further research is required to explore the transferability of such a concept to other clinical settings.