17 results on '"Yamani, Laura Navika"'
Search Results
2. Seroprevalence of SARS-CoV-2 anti-spike IgG antibody among COVID-19 vaccinated individuals residing in Surabaya, East Java, Indonesia
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Megasari, Ni Luh Ayu, primary, Yamani, Laura Navika, additional, Juniastuti, Juniastuti, additional, Lusida, Maria Inge, additional, and Mori, Yasuko, additional
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- 2023
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3. SARS-CoV-2 IgG antibody status in unvaccinated and 2-dose vaccinated Indonesians by AstraZeneca.
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YAMANI, LAURA NAVIKA, JUNIASTUTI, MEGASARI, NI LUH AYU, UTSUMI, TAKAKO, SAHILA, NUR, PANGESTIKA, ALIFIA SALMA, PUTRI, SERIUS MILIYANI DWI, YI LI, CHUNG, MARTINI, SANTI, ISFANDIARI, MUHAMMAD ATOILLAH, and LUSIDA, MARIA INGE
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MEDICAL personnel , *VACCINATION status , *VACCINATION , *IMMUNOGLOBULIN G , *ANTIBODY formation - Abstract
Indonesia began deploying a COVID-19 vaccine in January 2021, prioritising vaccination for high-risk groups such as healthcare workers, the elderly and those with comorbidities, and ending with the general public due to limited vaccine availability. Our study aimed to evaluate antibody response in Indonesians who had received two doses of the vaccine vs. those who had not. The study design was a cohort study involving 46 unvaccinated people and 23 people who had received the second dose of the AstraZeneca vaccine in three months. Methods used for the qualitative and quantitative detection of IgG antibodies included rapid RI-GHA and ELISA tests. Findings showed that positive IgG antibodies qualitatively detected by the rapid RI-GHA test were significantly higher in those vaccinated (60.9%) than in unvaccinated people (26.1%). Using the ELISA assay, all vaccinated individuals qualitatively showed positive antibodies (cut-off ≥4.33 BAU/ml), and the average quantitative titer of anti-SARS-CoV-2 s-RBD IgG was significantly higher in vaccinated (157.06±238.68 BAU/ml) than in unvaccinated (51.90±87.60 BAU/ml) individuals. Some unvaccinated individuals with no history of infection were found to have anti-SARS-CoV-2 antibodies that may have been previously asymptomatic, although their mean antibody titers were certainly lower than those in the 2-dose group. Approximately 56% of vaccinated individuals had antibody titers above 60 BAU/ml as a cut-off for protective threshold, a significantly higher proportion than unvaccinated individuals. In conclusion, vaccination with two doses AstraZeneca increased anti-SARS-CoV-2 antibodies which resulted in enhanced immunity against symptomatic COVID-19. [ABSTRACT FROM AUTHOR]
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- 2023
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4. Detection of Tuberculosis in Toddlers and its Risk Factor at East Perak Health Center Surabaya
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Azzahrain, Alifa Salsabila, primary, Afifah, Anisa Nur, additional, and Yamani, Laura Navika, additional
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- 2023
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5. Adverse Reactions of COVID-19 Vaccines: A Scoping Review of Observational Studies
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Dhamanti, Inge, primary, Suwantika, Auliya A, additional, Adlia, Amirah, additional, Yamani, Laura Navika, additional, and Yakub, Fitri, additional
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- 2023
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6. Adverse Reactions of COVID-19 Vaccines: A Scoping Review of Observational Studies
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Dhamanti,Inge, Suwantika,Auliya A, Adlia,Amirah, Yamani,Laura Navika, Yakub,Fitri, Dhamanti,Inge, Suwantika,Auliya A, Adlia,Amirah, Yamani,Laura Navika, and Yakub,Fitri
- Abstract
Inge Dhamanti,1â 3 Auliya A Suwantika,4â 6 Amirah Adlia,7 Laura Navika Yamani,8,9 Fitri Yakub10 1Department of Health Policy and Administration, Faculty of Public Health, Universitas Airlangga, Surabaya, Indonesia; 2Center for Patient Safety Research, Universitas Airlangga, Surabaya, Indonesia; 3School of Psychology and Public Health, La Trobe University, Melbourne, VIC, Australia; 4Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, Indonesia; 5Center of Excellence in Higher Education for Pharmaceutical Care Innovation, Universitas Padjadjaran, Bandung, Indonesia; 6Center for Health Technology Assessment, Universitas Padjadjaran, Bandung, Indonesia; 7Department of Pharmaceutics, School of Pharmacy, Institut Teknologi Bandung, Bandung, Indonesia; 8Division Epidemiology, Faculty of Public Health, Universitas Airlangga, Surabaya, Indonesia; 9Research Center on Global Emerging and Re-Emerging Infectious Diseases, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia; 10Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Skudai, MalaysiaCorrespondence: Inge Dhamanti, Tel +62315920948, Email inge-d@fkm.unair.ac.idAbstract: The COVID-19 pandemic had a severe global impact. A range of campaigns and activities, including vaccines, are being implemented to counteract this pandemic. Using observational data, the goal of this scoping review is to identify adverse events connected with COVID-19 vaccinations. We conduct a scoping study and searched three databases from the start of the COVID-19 pandemic in 2020 through June 2022. Based on our criteria and searched keywords, the review included eleven papers in total, with the majority of the studies being conducted in developed countries. The study populations varied and included general community populations, healthcare professionals, military forces, and patients with systemic lupus and cancer. This study includes vacc
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- 2023
7. Seroprevalence of SARS-CoV-2 anti-spike IgG antibody among COVID-19 vaccinated individuals residing in Surabaya, East Java, Indonesia.
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Ni Luh Ayu Megasari, Yamani, Laura Navika, Juniastuti, Juniastuti, Lusida, Maria Inge, and Yasuko Mori
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MEDICAL personnel ,BOOSTER vaccines ,COVID-19 ,IMMUNOGLOBULINS ,VACCINATION ,IMMUNOGLOBULIN G - Abstract
Background: To limit the SARS-CoV-2 transmission, the Indonesian government launched a COVID-19 vaccination program in January 2021. Studies on the clinical treatment and implementation of COVID-19 vaccination have shown promising results; however, it is necessary to estimate the effectiveness of the vaccines. With the ongoing COVID-19 pandemic, studies have highlighted the impact of COVID-19 vaccines, especially CoronaVac, on Indonesian healthcare workers. To get a better picture of how the vaccines work in Indonesia, it is necessary to estimate the prevalence of SARS-CoV-2 anti-S IgG antibody induced by the COVID-19 vaccine in individuals who have already received two-to-three doses of vaccines. Materials and Methods: Four-hundred and ninety-six whole-blood samples were collected from participants residing in Surabaya, East Java, Indonesia, who received a minimum of a two-dose COVID-19 vaccine. Serums were then isolated from the blood and subjected to detect SARS-CoV-2 anti-S IgG antibodies using a lateral flow immunochromatographic assay. Results: The prevalence of positive anti-S-IgG antibodies was 91.7% (455/496) in all participants receiving a minimum of a two-dose COVID-19 vaccine. As many as 209 (85.3%) and 141 (96.6%) participants were seropositive for receiving CoronaVac and AstraZeneca, respectively. Meanwhile, all participants receiving two-dose CoronaVac with one booster dose of Moderna (105/100%) were seropositive (p < 0.05). Age, comorbidity, and time after the last vaccine were significantly correlated with seropositivity (p < 0.05). Conclusion: Different vaccines might produce different antibody responses. Adopting a stronger policy regarding the administration of booster doses might be beneficial to elicit positive anti-S-IgG antibodies, especially among older individuals, those with comorbid diseases, and those with a longer time after the second vaccination dose. [ABSTRACT FROM AUTHOR]
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- 2023
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8. Different Variants in Reverse Transcriptase Domain Determined by Ultra-deep Sequencing in Treatment-naive and Treated Indonesian Patients Infected with Hepatitis B Virus
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Wasityastuti, Widya, Yano, Yoshihiko, Widasari, Dewiyani Indah, Yamani, Laura Navika, Ratnasari, Neneng, Heriyanto, Didik Setyo, Okada, Rina, Tanahashi, Toshihito, Murakami, Yoshiki, Azuma, Takeshi, and Hayashi, Yoshitake
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- 2016
9. Seroepidemiological study of SARS-CoV-2 infection in East Java, Indonesia.
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Megasari, Ni Luh Ayu, Utsumi, Takako, Yamani, Laura Navika, Juniastuti, Gunawan, Emily, Furukawa, Koichi, Nishimura, Mitsuhiro, Lusida, Maria Inge, and Mori, Yasuko
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COVID-19 ,SARS-CoV-2 ,PANDEMICS ,MEDICAL personnel ,COVID-19 pandemic ,HEALTH facilities - Abstract
Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic, including Indonesia. However, there are only limited data regarding the precise prevalence of the COVID-19 pandemic in Indonesia. Here, to estimate the magnitude of SARS-CoV-2 infection in East Java, Indonesia, we investigated the prevalence of immunoglobulin G (IgG) antibodies. We enrolled 1,819 individuals from June to December 2020 and observed that the subjects' overall prevalence of IgG antibody to SARS-CoV-2 was 11.4% (207/1,819). The prevalence of anti-SARS-CoV-2 antibodies differed significantly between the job/occupation groups (P = 0.0001). A greater prevalence of IgG was detected in laboratory technicians (who take samples from suspected cases and deal with polymerase chain reaction [PCR] procedures, 22.2%) compared to medical personnel who see and take direct care of patients with COVID-19 (e.g., physicians and nurses, 6.0%), other staff in medical facilities (2.9%), general population (12.1%) and non-COVID-19 patients (14.6%). The highest prevalence among age groups was in the 40–49-year-olds (14.8%), and the lowest prevalence was in the 20–29-year-olds (7.4%). However, the younger population still showed a higher prevalence than generally reported, suggesting greater exposure to the virus but less susceptibility to the disease. A geographical difference was also observed: a higher prevalence in Surabaya (13.1%) than in Jombang (9.9%). In conclusion, the COVID-19 outbreak among asymptomatic populations was characterized by a high prevalence of infection in East Java, Indonesia. [ABSTRACT FROM AUTHOR]
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- 2021
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10. Prevalence and Distribution of Rotavirus Genotypes Among Children With Acute Gastroenteritis in Areas Other Than Java Island, Indonesia, 2016–2018.
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Wahyuni, Rury Mega, Utsumi, Takako, Dinana, Zayyin, Yamani, Laura Navika, Juniastuti, Wuwuti, Ishak Samuel, Fitriana, Elsa, Gunawan, Emily, Liang, Yujiao, Ramadhan, Fitratul, Soetjipto, Lusida, Maria Inge, and Shoji, Ikuo
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GASTROENTERITIS ,NOROVIRUS diseases ,ROTAVIRUSES ,GENOTYPES ,CHILD patients ,ISLANDS ,SEQUENCE analysis - Abstract
Group A rotaviruses (RVAs) are the leading cause of acute gastroenteritis, which is often associated with severe symptoms in children under 5 years old. Genetic reassortments and interspecies transmission commonly occur, resulting in a great diversity of RVA circulating in the world. The aim of this study is to determine the prevalence and distribution of RVA genotypes among children in Indonesia over the years 2016–2018 across representative areas of the country. Stool samples were collected from 202 pediatric patients with acute gastroenteritis in three regions of Indonesia (West Nusa Tenggara, South Sumatra, and West Papua) in 2016–2018. Rotavirus G and P genotypes were determined by reverse transcription PCR (RT-PCR) and direct sequencing analysis. The prevalences of RVA in South Sumatra (55.4%) and West Papua (54.0%) were significantly higher than that in East Java (31.7%) as determined in our previous study. The prevalence in West Nusa Tenggara (42.6%) was the lowest among three regions, but higher than that in East Java. Interestingly, equine-like G3 rotavirus strains were found as predominant strains in South Sumatra in 2016 and in West Papua in 2017–2018. Moreover, the equine-like G3 strains in South Sumatra detected in 2016 were completely replaced by human G1 and G2 in 2018. In conclusion, RVA infection in South Sumatra and West Papua was highly endemic. Equine-like G3 strains were also spread to South Sumatra (West Indonesia) and West Papua (East Indonesia), as well as Java Island. Dynamic change in rotavirus genotypes from equine-like G3 to human genotypes was also observed. Continuous monitoring may be warranted in isolated areas in Indonesia. [ABSTRACT FROM AUTHOR]
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- 2021
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11. Equine-like G3 rotavirus strains as predominant strains among children in Indonesia in 2015–2016
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1000010364051, Utsumi, Takako, Wahyuni, Rury Mega, Doan, Yen Hai, Dinana, Zayyin, Soegijanto, Soegeng, Fujii, Yoshiki, Juniastuti, Yamani, Laura Navika, 1000070778414, Matsui, Chieko, Deng, Lin, 1000090403203, Abe, Takayuki, Soetjipto, Lusida, Maria Inge, Ishii, Koji, Shimizu, Hiroyuki, Katayama, Kazuhiko, 1000040356241, Shoji, Ikuo, 1000010364051, Utsumi, Takako, Wahyuni, Rury Mega, Doan, Yen Hai, Dinana, Zayyin, Soegijanto, Soegeng, Fujii, Yoshiki, Juniastuti, Yamani, Laura Navika, 1000070778414, Matsui, Chieko, Deng, Lin, 1000090403203, Abe, Takayuki, Soetjipto, Lusida, Maria Inge, Ishii, Koji, Shimizu, Hiroyuki, Katayama, Kazuhiko, 1000040356241, and Shoji, Ikuo
- Abstract
Rotavirus A (RVA) is a major cause of acute gastroenteritis in humans and animals worldwide. As a result of the segmented nature of the rotavirus genome, genetic reassortment commonly occurs. This study aims to clarify the genetic characteristics of RVAs circulating in Indonesia. From June 2015 through August 2016, stool samples were collected from 134 children aged <5 years (71 male and 63 female) with acute gastroenteritis who were inpatients at a private hospital in Surabaya, Indonesia. All stool samples were screened for RVA antigen using immunochromatography. Forty-two samples (31.3%, 42/134) were RVA antigen-positive. All RVA positive samples tested showed the unusual combinations of G3P[8] (n = 36) and G3P[6] (n = 3) with a short RNA pattern by G/P typing and polyacrylamide gel electrophoresis (PAGE). Whole genome analysis by next-generation sequencing (NGS) was performed for 11 strains to determine the RVA genotypes. Eleven rotavirus strains were found to carry a DS-like genetic backbone; nine strains showed a G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 genome constellation, which was recently reported in Australia, Hungary, Spain and Brazil; as well, two strains showed a G3-P[6]-I2-R2-C2-M2-A2-N2-T2-E2-H2 genome constellation. The phylogenetic tree based on the VP7 gene showed that all 11 strains were classified as equine-like G3, which is genetically distinct and different in origin from typical human G3 strains. The phylogenetic tree based on the NSP4 gene showed that six strains were classified as bovine-like strain and the remaining five were classified as human strain. In conclusion, we identified the strains which are intergenogroup reassortants containing an equine-like G3 VP7, a P[8])/P[6] VP4, with a DS-1-like genetic backbone. These findings suggest that equine-like G3P[8] and P[6] RVA strains have been circulating in the Indonesian population for at least 1 year and probably longer, indicating a diversity of RVAs in this area.
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- 2018
12. Analysis of hepatitis B virus genotype and gene mutation in patients with advanced liver disease in East Kalimantan, Indonesia.
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Wahyuni, Rury Mega, Utsumi, Takako, Juniastuti, Yano, Yoshihiko, Murti, Ignatia Sinta, Amin, Mochamad, Yamani, Laura Navika, Istimagfiroh, Anittaqwa, Purwono, Priyo Budi, Soetjipto, Lusida, Maria Inge, and Hayashi, Yoshitake
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CHRONIC hepatitis B ,HEPATITIS B virus ,HEPATITIS associated antigen ,LIVER diseases ,GENOTYPES ,VIRAL variation - Abstract
Liver cirrhosis (LC) and hepatocellular carcinoma (HCC) are life-threatening conditions frequently associated with chronic hepatitis B virus (HBV) infection in Asian countries, including Indonesia. HBV genotypes and several specific mutations are associated with disease progression. To clarify the geographical variation in viral characteristics, HBV genotypes and gene mutations were investigated in patients with advanced liver disease (ALD) in Samarinda, East Kalimantan, Indonesia. Sera were collected from 41 patients with ALD at Abdul Wahab Sjahranie Hospital and HBV carriers from Red Cross Center blood bank in Samarinda, and screened for hepatitis B surface antigen and hepatitis B e-antigen. Liver function data were obtained from the medical records from each patient. HBV genotype and gene mutations were determined by polymerase chain reaction sequencing. Analysis of HBV isolates indicated that genotype B was the most frequent genotype, at 85.4 and 97.8%, followed by C, at 14.6 and 2.2%, in patients with ALD and in HBV carriers, respectively. The C1505A mutation in X region, T1753V and A1762T/G1764A mutations in the basal core promoter region and C1858T in precore (PC) region were frequent and only detected in patients with ALD (28.9, 40, 73.5 and 17.6%, respectively), whereas the G1896A mutation in the PC region was frequently detected in HBV carriers. The presence of HBV genotype B and certain HBV gene mutations were characteristic of patients with ALD in East Kalimantan. [ABSTRACT FROM AUTHOR]
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- 2019
13. THE PRELIMINARY STUDY OF ANTIOXIDANT ACTIVITY FROM XYLO-OLIGOSACCHARIDE OF CORNCOB (ZEA MAYS) HYDROLYSIS PRODUCT WITH ENDO-β-XYLANASE ENZYME
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Yamani, Laura Navika, primary, Kristanti, Alfinda Novi, additional, and Puspaningsih, Ni Nyoman, additional
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- 2016
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14. Ultradeep Sequencing for Detection of Quasispecies Variants in the Major Hydrophilic Region of Hepatitis B Virus in Indonesian Patients
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Yamani, Laura Navika, Yano, Yoshihiko, Utsumi, Takako, Juniastuti, Wandono, Hadi, Widjanarko, Doddy, Triantanoe, Ari, Wasityastuti, Widya, Liang, Yujiao, Okada, Rina, Tanahashi, Toshihito, Murakami, Yoshiki, Azuma, Takeshi, Soetjipto, Lusida, Maria Inge, and Hayashi, Yoshitake
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ABSTRACTQuasispecies of hepatitis B virus (HBV) with variations in the major hydrophilic region (MHR) of the HBV surface antigen (HBsAg) can evolve during infection, allowing HBV to evade neutralizing antibodies. These escape variants may contribute to chronic infections. In this study, we looked for MHR variants in HBV quasispecies using ultradeep sequencing and evaluated the relationship between these variants and clinical manifestations in infected patients. We enrolled 30 Indonesian patients with hepatitis B infection (11 with chronic hepatitis and 19 with advanced liver disease). The most common subgenotype/subtype of HBV was B3/adw (97%). The HBsAg titer was lower in patients with advanced liver disease than that in patients with chronic hepatitis. The MHR variants were grouped based on the percentage of the viral population affected: major, =20% of the total population; intermediate, 5% to <20%; and minor, 1% to <5%. The rates of MHR variation that were present in the major and intermediate viral population were significantly greater in patients with advanced liver disease than those in chronic patients. The most frequent MHR variants related to immune evasion in the major and intermediate populations were P120Q/T, T123A, P127T, Q129H/R, M133L/T, and G145R. The major population of MHR variants causing impaired of HBsAg secretion (e.g., G119R, Q129R, T140I, and G145R) was detected only in advanced liver disease patients. This is the first study to use ultradeep sequencing for the detection of MHR variants of HBV quasispecies in Indonesian patients. We found that a greater number of MHR variations was related to disease severity and reduced likelihood of HBsAg titer.
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- 2015
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15. Association Between HBx Variations and Development of Severe Liver Disease Among Indonesian Patients.
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Putri WA, Yano Y, Yamani LN, Lusida MI, Soetjipto, Liang Y, Mardian Y, Wasityastuti W, and Hayashi Y
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- Hepatitis B virus immunology, Humans, Immune Evasion, Viral Regulatory and Accessory Proteins, Liver Neoplasms etiology, Mutation, Trans-Activators genetics
- Abstract
Multi-site mutations in the hepatitis B virus (HBV) X gene are often found in patients with advanced liver diseases such as liver cirrhosis and hepatocellular carcinoma. It has been reported that modifications in the X protein play crucial roles in the development of HBV-related severe liver disease. However, the prevalence of genetic variations in Indonesian strains has not been systematically assessed. In this study, we sought to investigate the profile of nonsynonymous mutations in the X gene. Overall, 114 Indonesian HBV strains, including 12 in-house samples, were retrieved from GenBank. The mutation frequency in the X gene was compared among strains obtained from patients with chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. The mutation frequencies of the domain and basal core promoter regions were significantly greater in advanced liver diseases compared with chronic hepatitis. In addition, the double mutation K130M/V131I and the triple mutation N88V/K130M/V131I were associated with a 2.5 times higher risk of advanced liver disease. However, the roles of two novel X gene mutations (A12S/T and L16F/P) on hepatocarcinogenesis are unclear relative to wild-type X gene. In conclusion, the development of multi-site mutations in the X gene may represent a strategy by which HBV can escape immune surveillance and thus contribute to hepatocarcinogenesis, even though the biological roles of some variants remain unclear.
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- 2019
16. Molecular Epidemiology and Clinical Features of Rotavirus Infection Among Pediatric Patients in East Java, Indonesia During 2015-2018: Dynamic Changes in Rotavirus Genotypes From Equine-Like G3 to Typical Human G1/G3.
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Athiyyah AF, Utsumi T, Wahyuni RM, Dinana Z, Yamani LN, Soetjipto, Sudarmo SM, Ranuh RG, Darma A, Juniastuti, Raharjo D, Matsui C, Deng L, Abe T, Doan YH, Fujii Y, Shimizu H, Katayama K, Lusida MI, and Shoji I
- Abstract
Group A rotavirus (RVA) is the most important cause of severe gastroenteritis among children worldwide, and effective RVA vaccines have been introduced in many countries. Here we performed a molecular epidemiological analysis of RVA infection among pediatric patients in East Java, Indonesia, during 2015-2018. A total of 432 stool samples were collected from hospitalized pediatric patients with acute gastroenteritis. None of the patients in this cohort had been immunized with an RVA vaccine. The overall prevalence of RVA infection was 31.7% (137/432), and RVA infection was significantly more prevalent in the 6- to 11-month age group than in the other age groups ( P < 0.05). Multiplex reverse transcription-PCR (RT-PCR) revealed that the most common G-P combination was equine-like G3P[8] (70.8%), followed by equine-like G3P[6] (12.4%), human G1P[8] (8.8%), G3P[6] (1.5%), and G1P[6] (0.7%). Interestingly, the equine-like strains were exclusively detected until May 2017, but in July 2017 they were completely replaced by a typical human genotype (G1 and G3), suggesting that the dynamic changes in RVA genotypes from equine-like G3 to typical human G1/G3 in Indonesia can occur even in the country with low RVA vaccine coverage rate. The mechanism of the dynamic changes in RVA genotypes needs to be explored. Infants and children with RVA-associated gastroenteritis presented more frequently with some dehydration, vomiting, and watery diarrhea, indicating a greater severity of RVA infection compared to those with non-RVA gastroenteritis. In conclusion, a dynamic change was found in the RVA genotype from equine-like G3 to a typical human genotype. Since severe cases of RVA infection were prevalent, especially in children aged 6 to 11 months or more generally in those less than 2 years old, RVA vaccination should be included in Indonesia's national immunization program.
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- 2019
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17. Profile of Mutations in the Reverse Transcriptase and Overlapping Surface Genes of Hepatitis B Virus (HBV) in Treatment-Naïve Indonesian HBV Carriers.
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Yamani LN, Yano Y, Utsumi T, Wasityastuti W, Rinonce HT, Widasari DI, Juniastuti, Lusida MI, Soetjipto, and Hayashi Y
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- Amino Acid Substitution, DNA Mutational Analysis, Genome, Viral, Genotype, Hepatitis B Surface Antigens genetics, Hepatitis B virus classification, Hepatitis B virus enzymology, Humans, Indonesia epidemiology, Phylogeography, Prevalence, Carrier State, Drug Resistance, Viral, Hepatitis B epidemiology, Hepatitis B virology, Hepatitis B virus genetics, Mutation, RNA-Directed DNA Polymerase genetics
- Abstract
Mutations in the reverse transcriptase (RT) region of the hepatitis B virus (HBV) genome are an important factor in low therapeutic effectiveness. Nonetheless, the prevalence of these mutations in HBV strains isolated previously in Indonesia has not been systematically examined. Therefore, in this study, we investigated the profile of mutations in the RT region and the associations of these mutations with amino acid changes in the surface protein in the virus of treatment-naïve Indonesian HBV carriers. Overall, 96 sequences of the full-length Indonesian HBV genomes (genotype B, n = 54; genotype C, n = 42) were retrieved from the National Center for Biotechnology Information. Naturally occurring primary and/or compensatory drug resistance mutations were found in 6/54 (11.1%) genotype B strains and in 1/42 (2.4%) genotype C strains. The potential mutations underlying resistance to a nucleos(t)ide analog and/or pretreatment mutations were more frequent in both genotypes but more frequent in genotype C strains than in genotype B strains. The A-B interdomain region in the RT gene was more frequently mutated in genotype C than in genotype B (3.51 ± 2.53 vs. 1.08 ± 1.52, P < 0.001). Knowledge about the mutational profiles of the RT gene and changes in the surface protein may help clinicians to select the most appropriate antiviral drug and vaccination or HBV immunoglobulin regimen for management of HBV infection in Indonesia.
- Published
- 2017
- Full Text
- View/download PDF
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