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Although more than 100 genome sequences of Pasteurella multocida are available, comprehensive and complete genome sequence analysis is limited. This study describes the analysis of complete genome sequence and pathogenomics of P. multocida strain PMTB2.1. The genome of PMTB2.1 has 2176 genes with more than 40 coding sequences associated with iron regulation and 140 virulence genes including the complete tad locus. The tad locus includes several previously uncharacterized genes such as flp2, rcpC and tadV genes. A transposable phage resembling to Mu phages was identified in P. multocida that has not been identified in any other serotype yet. The multi-locus sequence typing analysis assigned the PMTB2.1 genome sequence as type ST101, while the comparative genome analysis showed that PMTB2.1 is closely related to other P. multocida strains with the genomic distance of less than 0.13. The expression profiling of iron regulating-genes of PMTB2.1 was characterized under iron-limited environment. Results showed significant changes in the expression profiles of iron-regulating genes (p < 0.05) whereas the highest expression of fecE gene (281 fold) at 30 min suggests utilization of the outer-membrane proteins system in iron acquisition at an early stage of growth. This study showed the phylogenomic relatedness of P. multocida and improved annotation of important genes and functional characterization of iron-regulating genes of importance to the bacterial growth.

Pasteurella multocida causes pneumonic pasteurellosis and hemorrhagic septicemia (HS) in large ruminants. In this study, we determined the complete genome sequence of P. multocida strain PMTB2.1 capsular serotype A isolated from buffaloes that died of septicemia.

Pasteurella multocida serotypes B:2 and E:2 are the main causative agents of ruminant hemorrhagic septicemia in Asia and Africa, respectively. Pasteurella multocida strain PMTB was isolated from a buffalo with hemorrhagic septicemia and has been determined to be serotype B:2. Here we report the draft genome sequence of strain PMTB.

Pasteurella multocida serotype B:2 and E:2 are the main causative agents of hemorrhagic septicaemia (HS) in ruminants. Hemorrhagic septicemia is an acute,fatal and septicemic disease of economic importance in tropical regions of the world especially in Asian and African countries. To date, the available vaccines are unable to induce long term immunity and require individual injection which is not feasible since animals normally live in large wild herds or kept semi-wild by the farmers. The molecular basis for pathogenesis of HS caused by P. multocida during natural infections is still vague. Thus, there is an imminent need for whole genome sequencing of HS causing strain as the first step towards understanding of the pathogenicity of P. multocida in causing HS in infected ruminants. Hence, in previous study, the genome of P. multocida serotype B:2 strain PMTB isolated from a dead buffalo during an outbreak of HS in Kelantan, Malaysia, year 2003 was sequenced using Illumina Genome Analyzer. Approximately 7.2 million single end reads with data size 250 MB and an average length of 35 bp was generated, providing ~100 fold genome coverage. De novo assembly using software Velvet produced scaffold with 2191094 bp in length and 89 genome gaps. In this study,Polymerase Chain Reaction (PCR) sequencing approach was used and successfully closed 80 genome gaps with 9 unresolved gaps. The 9 remaining unresolved gaps were due to the large length of the gaps, repeat sequence related or missasemblies of contigs. The predicted total length of the remaining unresolved gap was about 215 k bp, which is impossible to amplify in PCR assay even by using pfu polymerase that specialize in long range PCR. The misassembled contigs and repeat sequence related gaps caused no amplification in PCR assay or the sequences obtained were unable to map to the targeted region. The improved scaffold with 2203419 bp in length with G + C content of 40.46 % was submitted to Prokaryotic Genome Annotation Pip