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18 results on '"Yin, Huancai"'

10. Assessment of Clofazimine and TB47 Combination Activity against Mycobacterium abscessus Using a Bioluminescent Approach

Author

Liu, Yang, primary, Tan, Yaoju, additional, Islam, M. Mahmudul, additional, Cao, Yuanyuan, additional, Lu, Xiaoyun, additional, Zeng, Sheng, additional, Hameed, H. M. Adnan, additional, Zhou, Peipei, additional, Cai, Xingshan, additional, Wang, Shuai, additional, Mugweru, Julius N., additional, Zhang, Guoliang, additional, Yin, Huancai, additional, Liu, Jianxiong, additional, Nuermberger, Eric, additional, and Zhang, Tianyu, additional

Published
2020
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16. Discrimination of human and nonhuman blood using Raman spectroscopy with self-reference algorithm

Author

Haiyi Bian, Xiaodong Wu, Peng Wang, Yuguo Tang, Jing Gao, Ning Wang, Yin Huancai, Tian Yubing, Jun Wang, and Bai Pengli

Subjects
Materials science, Biomedical Engineering, 02 engineering and technology, Spectrum Analysis, Raman, 01 natural sciences, Biomaterials, symbols.namesake, Dogs, Species Specificity, Animals, Humans, Statistical analysis, Least-Squares Analysis, Lasers, Forensic Sciences, 010401 analytical chemistry, 021001 nanoscience & nanotechnology, Atomic and Molecular Physics, and Optics, Rats, 0104 chemical sciences, Electronic, Optical and Magnetic Materials, Blood, Laser intensity, symbols, Rabbits, 0210 nano-technology, Raman spectroscopy, Algorithm, True positive rate, Algorithms
Abstract

We report a self-reference algorithm to discriminate human and nonhuman blood by calculating the ratios of identification Raman peaks to reference Raman peaks and choosing appropriate threshold values. The influence of using different reference peaks and identification peaks was analyzed in detail. The Raman peak at 1003 cm-1 was proved to be a stable reference peak to avoid the influencing factors, such as the incident laser intensity and the amount of sample. The Raman peak at 1341 cm-1 was found to be an efficient identification peak, which indicates that the difference between human and nonhuman blood results from the C-H bend in tryptophan. The comparison between self-reference algorithm and partial least square method was made. It was found that the self-reference algorithm not only obtained the discrimination results with the same accuracy, but also provided information on the difference of chemical composition. In addition, the performance of self-reference algorithm whose true positive rate is 100% is significant for customs inspection to avoid genetic disclosure and forensic science.

Published
2017

17. Assessment of Clofazimine and TB47 Combination Activity against Mycobacterium abscessusUsing a Bioluminescent Approach

Author

Liu, Yang, Tan, Yaoju, Islam, M. Mahmudul, Cao, Yuanyuan, Lu, Xiaoyun, Zeng, Sheng, Hameed, H. M. Adnan, Zhou, Peipei, Cai, Xingshan, Wang, Shuai, Mugweru, Julius N., Zhang, Guoliang, Yin, Huancai, Liu, Jianxiong, Nuermberger, Eric, and Zhang, Tianyu

Abstract

Mycobacterium abscessusis intrinsically resistant to most antimicrobial agents. The emerging infections caused by M. abscessusand the lack of effective treatment call for rapid attention. Here, we intended to construct a selectable marker-free autoluminescent M. abscessusstrain (designated UAlMab) as a real-time reporter strain to facilitate the discovery of effective drugs and regimens for treating M. abscessus. The UAlMab strain was constructed using the dif/Xer recombinase system.

Published
2020
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18. Development of a class-specific immunochromatographic strip test for the rapid detection of organophosphorus pesticides with a thiophosphate group.

Author

Su J, Yang H, Chen J, Yin H, Tang R, Xie Y, Song K, Huyan T, Wang H, Wang W, and Xue X

Subjects
Animals, Antibodies, Monoclonal immunology, Chlorpyrifos, Enzyme-Linked Immunosorbent Assay, Female, Haptens chemistry, Haptens metabolism, Mice, Mice, Inbred BALB C, Reference Standards, Gold Colloid chemistry, Organothiophosphorus Compounds analysis, Pesticide Residues analysis, Phosphates chemistry, Reagent Strips
Abstract

In recent decades, organophosphorus (OP) pesticides are widely used in agricultural and domestic fields. There is increasing demand for more rapid and economical methods of detecting pesticide residues. We have developed a gold immunochromatography assay (GICA) strip test for detecting a group of OP pesticides based on monoclonal antibodies (MAb) against a generic hapten, O,O-diethyl O-(4-carboxy-3-methylphenyl) phosphorothioate. The MAb showed desirable properties for use in indirect competitive ELISA, and the minimum IC50 value (93 ng/mL) was observed in chlorpyrifos-ethyl curve. The partially purified monoclonal antibodies were conjugated with colloidal gold particles. The conjugation of the colloidal gold and antibodies was monitored by ultraviolet-visible (UV-vis) spectroscopy and fluorescence spectroscopy, while the transmission electron microscopy (TEM) images were used to characterize the particle size and shape of the conjugates. Dynamic light scattering (DLC) was applied as a less expensive and faster method to substitute TEM. The colloidal gold and antibody conjugates were used for the production of a GICA strip test to detect OP pesticides. Preliminary assessment suggests that the OP pesticide, chlorpyrifos-ethyl (CE), in environmental samples could be detected by the GICA strip. This assay could potentially be used for screening large volume of samples and on-spot monitoring before the precise analysis. But the homogeneity of the detection needs to be improved further.

Published
2010
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