Search

The purpose of the present study was to investigate whether preserving capsule and primary intraocular lens implantation in vitrectomy could influence the therapeutic effect and safety in the treatment of post-traumatic endophthalmitis. A prospective, randomized, controlled trial was performed in this study. A total of 89 patients with post-traumatic endophthalmitis that occurred between January 2008 and December 2010 were randomly divided into two groups: 45 patients underwent complete vitrectomy with preserved capsule and primary intraocular lens implantation, and 44 patients underwent complete vitrectomy with lensectomy and without intraocular lens implantation. There were no significant differences in patients’ age, sex, injuries in detail, intraocular tamponade, culture-proven bacteria, and preoperative and postoperative visual acuity between two groups. No recurrence of bacterial infection was found in any case in both groups, and there was no severe complications happened in two groups during follow-up. Our results suggested that preserving capsule and primary intraocular lens implantation with complete vitrectomy in post-traumatic endophthalmitis was a safe and feasible surgery procedure with benefits in postoperative visual quality, which could be recommended as an alternative therapy. Key words: Post-traumatic endophthalmitis, intraocular lens implantation, capsule, vitrectomy, infection.

Purpose To investigate the mRNA and protein expression of nitric oxide synthase (NOS) in human ciliary bodies in vitro. The effect of the adenylcyclase activator forskolin and/or the α2-adrenergic agonist brimonidine (an ocular hypotensive agent that inhibits aqueous humor formation) on NOS mRNA or protein expression was also studied. Methods Frozen human ciliary bodies obtained from local eye bank were thawed and incubated with 0.1 mM forskolin for 24 h in the absence or in the presence of 100 μM brimonidine. The mRNA and protein expression of three NOS isoforms (neuronal NOS or nNOS, inducible NOS or iNOS, endothelial NOS or eNOS) were assessed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. Results mRNA and protein expression of three NOS isoforms were detected in human ciliary bodies. Forskolin significantly up-regulated the mRNA and protein expression of nNOS, but not that of iNOS or of eNOS. In the presence of brimonidine, the forskolin-induced up-regulation of nNOS mRNA or protein expression was significantly inhibited. Conclusions In human ciliary body (where aqueous humor is produced), brimonidine inhibits the up-regulation of nNOS expression induced by forskolin.