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1. Production of Polyunsaturated Fatty Acids by Mucor recurvus sp. with Sugarcane Molasses as the Carbon Source

Author

Zhi-Nian Deng, Nan Li, Yong-Ling Qin, Chun-Lan Chen, and Zhi-Qun Liang

Subjects
Mucor recurvus sp., polyunsaturated fatty acids, sugarcane molasses, fermentation, cultivation condition, fatty acid analysis, Biotechnology, TP248.13-248.65, Food processing and manufacture, TP368-456
Abstract

Sugarcane molasses is rich in nutriments and vitamins. It may be used as the carbon source for the production of polyunsaturated fatty acids (PUFA) by Mucor recurvus sp. Using sugarcane molasses, the effects of fermentation parameters and media components on polyunsaturated fatty acid production through both linear and orthogonal array experiments were investigated. The best fermentation conditions for PUFA production were found as follows: 15 % sugarcane molasses, pH=6.0, 28 °C, 5 days, 160 rpm. It was also found that molasses and urea enhanced PUFA production with the optimal carbon to nitrogen (C/N) ratio of 35. Under the most favourable conditions, the total lipid content at 7.13 g/L and PUFA up to 5.74 g/L including (0.82±0.05) g/L of linolenic acid (LA), (1.35±0.02) g/L of γ-linolenic acid (GLA), (0.17±0.06) g/L of α-linolenic acid (ALA), (0.57±0.06) g/L of arachidonic acid (ARA), (0.46±0.07) g/L of eicosapentaenoic acid (EPA) and (0.34±0.08) g/L of docosahexaenoic acid (DHA) were obtained. Our study suggests that sugarcane molasses is a superior alternative carbon source for industrial PUFA production.

Published
2008

2. Synthesis and crystal structure of trans-tetraaqua-bis(3-(((7-hydroxy-3-(4-hydroxy-3-sulfonatophenyl)-4-oxo-4H-chromen-8-yl)methyl)ammonio)propanoato-κO)zinc(II) tetrahydrate, C38H48N2O26S2Zn

Author

Peng Zhang, Hai-Lin Chen, Yan-Jun Xie, Yong-Ling Qin, and Li-Wei Pan

Subjects
Tetrahydrate, 010405 organic chemistry, chemistry.chemical_element, Zinc, Crystal structure, Condensed Matter Physics, 01 natural sciences, Medicinal chemistry, 0104 chemical sciences, Inorganic Chemistry, 010404 medicinal & biomolecular chemistry, chemistry.chemical_compound, chemistry, General Materials Science
Abstract

C38H48N2O26S2Zn, monoclinic, P21/c (no. 14), a = 15.7219(7) Å, b = 8.0394(4) Å, c = 17.1968(8) Å, β = 92.210(4)°, V = 2171.97(18) Å3, Z = 2, Rgt(F) = 0.0395, wRref(F2) = 0.0951, T = 293(2) K.

Published
2018

3. Synthesis and crystal structure poly[aqua(μ3-2-(((7-hydroxy-3-(4-hydroxy-3-sulfonatophenyl)-4-oxo-4H-chromen-8-yl)methyl)ammonio)acetate-κ4 O,O′:O′′:O′′′) sodium] monohydrate, C18H18NNaO11S

Author

Ting Chen, Hong-Fang Lai, Xiao-Yuan Huang, Hai-Lin Chen, Yong-Ling Qin, and Yue-Ning Guo

Subjects
Inorganic Chemistry, 010405 organic chemistry, Chemistry, General Materials Science, Crystal structure, 010403 inorganic & nuclear chemistry, Condensed Matter Physics, 01 natural sciences, Medicinal chemistry, SODIUM MONOHYDRATE, 0104 chemical sciences
Abstract

C18H18NNaO11S, triclinic, P1̅ (no. 2), a = 8.2627(12) Å, b = 8.6490(11) Å, c = 14.1791(17) Å, α = 82.883(10)°, β = 76.954(12)°, γ = 69.729(12)°, V = 924.8(2) Å3, Z = 2, R gt(F) = 0.0574, wR ref(F 2) = 0.1609, T = 293(2) K.

Published
2018

4. Proizvodnja polinezasićenih masnih kiselina s pomoću plijesni Mucor recurvus sp. upotrebom melase šećerne trske kao izvora ugljika

Author

Nan Li, Zhi-Nian Deng, Yong-Ling Qin, Chun-Lan Chen, and Zhi-Qun Liang

Subjects
Mucor recurvus sp, polinezasićene masne kiseline, melasa šećerne trske, fermentacija, uvjeti uzgoja, analiza masnih kiselina, food and beverages, lipids (amino acids, peptides, and proteins), polyunsaturated fatty acids, sugarcane molasses, fermentation, cultivation condition, fatty acid analysis
Abstract

Sugarcane molasses is rich in nutriments and vitamins. It may be used as the carbon source for the production of polyunsaturated fatty acids (PUFA) by Mucor recurvus sp. Using sugarcane molasses, the effects of fermentation parameters and media components on polyunsaturated fatty acid production through both linear and orthogonal array experiments were investigated. The best fermentation conditions for PUFA production were found as follows: 15 % sugarcane molasses, pH=6.0, 28 °C, 5 days, 160 rpm. It was also found that molasses and urea enhanced PUFA production with the optimal carbon to nitrogen (C/N) ratio of 35. Under the most favourable conditions, the total lipid content at 7.13 g/L and PUFA up to 5.74 g/L including (0.82±0.05) g/L of linolenic acid (LA), (1.35±0.02) g/L of γ-linolenic acid (GLA), (0.17±0.06) g/L of α-linolenic acid (ALA), (0.57±0.06) g/L of arachidonic acid (ARA), (0.46±0.07) g/L of eicosapentaenoic acid (EPA) and (0.34±0.08) g/L of docosahexaenoic acid (DHA) were obtained. Our study suggests that sugarcane molasses is a superior alternative carbon source for industrial PUFA production., Melasa šećerne trske je bogata hranjivim tvarima i vitaminima. Upotrebljava se kao izvor ugljika za proizvodnju polinezasićenih masnih kiselina s pomoću plijesni Mucor recurvus sp. Linearnom i ortogonalnom metodom istražen je utjecaj uvjeta fermentacije i sastojaka podloge, uz dodatak melase šećerne trske, na proizvodnju polinezasićenih masnih kiselina. Najbolji uvjeti fermentacije za proizvodnju polinezasićenih masnih kiselina bili su: 15 % melase šećerne trske; pH=6,0; 28 °C; 5 dana i 160 rpm. Također je utvrđeno da dodatak melase i uree povećava proizvodnju pri optimalnom omjeru ugljika i dušika C/N=35. Pri optimalnim uvjetima proizvedeno je ukupno 7,13 g/L lipida i 5,74 g/L polinezasićenih masnih kiselina, od toga (0,82±0,05) g/L linolenske kiseline, (1,35±0,02) g/L γ-linolenske kiseline, (0,17±0,06) g/L α-linolenske kiseline, (0,57±0,06) g/L arahidonske kiseline, (0,46±0,07) g/L eikosapentenoične kiseline i (0,34±0,08) g/L dokosaheksaenoične kiseline. Ovo je istraživanje pokazalo da je melasa šećerne trske najbolji alternativni izvor ugljika za industrijsku proizvodnju polinezasićenih masnih kiselina.

Published
2008

5. Production of Polyunsaturated Fatty Acids by Mucor recurvus sp. with Sugarcane Molasses as the Carbon Source

Author

Nan Li, Zhi-Nian Deng, Yong-Ling Qin, Chun-Lan Chen, Zhi-Qun Liang, Nan Li, Zhi-Nian Deng, Yong-Ling Qin, Chun-Lan Chen, and Zhi-Qun Liang

Abstract

Sugarcane molasses is rich in nutriments and vitamins. It may be used as the carbon source for the production of polyunsaturated fatty acids (PUFA) by Mucor recurvus sp. Using sugarcane molasses, the effects of fermentation parameters and media components on polyunsaturated fatty acid production through both linear and orthogonal array experiments were investigated. The best fermentation conditions for PUFA production were found as follows: 15 % sugarcane molasses, pH=6.0, 28 °C, 5 days, 160 rpm. It was also found that molasses and urea enhanced PUFA production with the optimal carbon to nitrogen (C/N) ratio of 35. Under the most favourable conditions, the total lipid content at 7.13 g/L and PUFA up to 5.74 g/L including (0.82±0.05) g/L of linolenic acid (LA), (1.35±0.02) g/L of γ-linolenic acid (GLA), (0.17±0.06) g/L of α-linolenic acid (ALA), (0.57±0.06) g/L of arachidonic acid (ARA), (0.46±0.07) g/L of eicosapentaenoic acid (EPA) and (0.34±0.08) g/L of docosahexaenoic acid (DHA) were obtained. Our study suggests that sugarcane molasses is a superior alternative carbon source for industrial PUFA production., Melasa šećerne trske je bogata hranjivim tvarima i vitaminima. Upotrebljava se kao izvor ugljika za proizvodnju polinezasićenih masnih kiselina s pomoću plijesni Mucor recurvus sp. Linearnom i ortogonalnom metodom istražen je utjecaj uvjeta fermentacije i sastojaka podloge, uz dodatak melase šećerne trske, na proizvodnju polinezasićenih masnih kiselina. Najbolji uvjeti fermentacije za proizvodnju polinezasićenih masnih kiselina bili su: 15 % melase šećerne trske; pH=6,0; 28 °C; 5 dana i 160 rpm. Također je utvrđeno da dodatak melase i uree povećava proizvodnju pri optimalnom omjeru ugljika i dušika C/N=35. Pri optimalnim uvjetima proizvedeno je ukupno 7,13 g/L lipida i 5,74 g/L polinezasićenih masnih kiselina, od toga (0,82±0,05) g/L linolenske kiseline, (1,35±0,02) g/L γ-linolenske kiseline, (0,17±0,06) g/L α-linolenske kiseline, (0,57±0,06) g/L arahidonske kiseline, (0,46±0,07) g/L eikosapentenoične kiseline i (0,34±0,08) g/L dokosaheksaenoične kiseline. Ovo je istraživanje pokazalo da je melasa šećerne trske najbolji alternativni izvor ugljika za industrijsku proizvodnju polinezasićenih masnih kiselina.

Published
2008

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