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1. Development of Amperometric Hydrogen Peroxide Sensor Based on Horseradish Peroxidase-Immobilized Poly(Thiophene-co-EpoxyThiophene)

Author

Young-Taeg Lee, Chang-Ho Shin, Seong-Ho Choi, Ming-Hua Piao, and Hwa-Jung Kim

Subjects
Poly(Th-co-EpoxyTh) electrode, Horseradish peroxidase, Cyclic voltammetry, H2O2, Chemical technology, TP1-1185
Abstract

A modified electrode for hydrogen peroxide (H2O2) sensing was prepared via thiophene (Th) with epoxy group. Thiophene (EpoxyTh) with epoxy group was synthesized by reaction of 3-bromothiophene and glycidyl methacrylate (GMA) in acetonitrile according to Heck Reaction. The electrocopolymerization of Th and EpoxyTh was performed on the surface of indium tin oxide (ITO) electrode by cycling the potential between -1.0 and +2.5 V in mixture of thiophene (Th) and EpoxyTh. Poly(Th-co- EpoxyTh) grown onto the ITO electrode was successfully confirmed by SEM, AFM, and water contact angle analysis, respectively. Finally, the HRP was immobilized on the surface of poly(Th-co-EpoxyTh) electrode by covalent binding. The amperometric response of the HRP-immobilized poly(Th-co-EpoxyTh) electrode for H2O2 was examined by cyclic voltammetry (CV). The HRP-immobilized poly(Th-co-EpoxyTh) electrode showed linearity from 0.1 to 30 mM H2O2, good reproducibility, and long life time.

Published
2008

2. Development of Amperometric Hydrogen Peroxide Sensor Based on Horseradish Peroxidase-Immobilized Poly(Thiophene-co-EpoxyThiophene)

Author

Chang-Ho Shin, Ming-Hua Piao, Young-Taeg Lee, Seong-Ho Choi, and Hwa-Jung Kim

Subjects
Glycidyl methacrylate, Cyclic voltammetry, Chemistry, Communication, H2O2, Nanotechnology, lcsh:Chemical technology, Biochemistry, Atomic and Molecular Physics, and Optics, Amperometry, Horseradish peroxidase, Analytical Chemistry, Indium tin oxide, chemistry.chemical_compound, Poly(Th-co-EpoxyTh) electrode, Electrode, Thiophene, lcsh:TP1-1185, Electrical and Electronic Engineering, Hydrogen peroxide, Acetonitrile, Instrumentation, Nuclear chemistry
Abstract

A modified electrode for hydrogen peroxide (H2O2) sensing was prepared via thiophene (Th) with epoxy group. Thiophene (EpoxyTh) with epoxy group was synthesized by reaction of 3-bromothiophene and glycidyl methacrylate (GMA) in acetonitrile according to Heck Reaction. The electrocopolymerization of Th and EpoxyTh was performed on the surface of indium tin oxide (ITO) electrode by cycling the potential between -1.0 and +2.5 V in mixture of thiophene (Th) and EpoxyTh. Poly(Th-co- EpoxyTh) grown onto the ITO electrode was successfully confirmed by SEM, AFM, and water contact angle analysis, respectively. Finally, the HRP was immobilized on the surface of poly(Th-co-EpoxyTh) electrode by covalent binding. The amperometric response of the HRP-immobilized poly(Th-co-EpoxyTh) electrode for H2O2 was examined by cyclic voltammetry (CV). The HRP-immobilized poly(Th-co-EpoxyTh) electrode showed linearity from 0.1 to 30 mM H2O2, good reproducibility, and long life time.

Published
2008
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