3 results on '"Zhai Shana"'
Search Results
2. Diabetes mellitus - basic research
- Author
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Sara Cattaneo, Keisuke Satoh, Li Ying, Renate Koppensteiner, Cassia Toledo Bergamaschi, Akira Nishiyama, Ma. Lorena Roldán, Andrew P. Levy, Lislaine A. Wensing, Francesca D'Addio, Maki Takeuchi, Piergiorgio Messa, Benedita Sampaio-Maia, Shigetaka Yoshida, Yoko Saito, Andrea Remuzzi, Hitoshi Nakashima, Elisa Mieko Suemitsu Higa, Maho Watanabe, Fabiola Carrara, Caihong Zeng, Nakhoul Nakhoul, Margaret Gori Mouro, Daniela Corna, Rabea Asleh, Miki Nagase, Hideharu Tanaka, Liliana A. Monasterolo, Romina Pagotto, Michael Brownlee, Jun Okabe, Bi-Cheng Liu, Sho-ichi Yamagishi, Hyung Wook Kim, Mark E. Cooper, Gabriel Cao, Yasuo Kawaba, Carine Prisco Arnoni, Jonathan Barasch, Guoping Zheng, Min Li, Hirotaka Imamaki, Luciana G. Pereira, Akira Sugawara, Masakazu Kohno, Yixin Qian, Tomoko Kawanishi, Manuel Pestana, Hui-Ping Chen, Franck Molina, Silvia Armelloni, Rui Alves, Yanna Dou, Wei-Song Qin, Shinichi Okada, Juliana L. Dreyfuss, Zhangsuo Liu, Seok Joon Shin, Hidenori Arai, Casandra Margarita Monzón, Daniela Rottoli, Reza Abdi, Shan Mou, Alexandre H. Campos, Toru Kita, Li Ya, Hiroshi Kanamori, Yong Gu, Thomas Hoertenhuber, Natsuko Iga, Catia Cerqueira, Shigeru Shibata, Marina Munoz, Maria Pia Rastaldi, Mi Lee, Joanne K. Ferguson, Chi-Chih Hung, Andrea Vergani, Fabio Sangalli, Yiping Wang, Elena Gagliardini, Hassan Kulaksiz, Yukako Kinoshita, Nicolas Salvetat, Li Zhang, Tullio Bertani, Antonio Cabrita, Young Ae Kang, Vincent Lee, Takashige Kuwabara, Daisuke Nakano, Mollie Jurewicz, Toshiro Fujita, Hyun Wha Chung, Rachel Miller-Lotan, Kiyoshi Mori, Muh Geot Wong, Motoaki Saito, Lei-Shi Li, Paula Serrao, Eman El Eter, Yan Qiu, Yi-Mei Hong, Hung-Chun Chen, Christina Maeda Takiya, Romano Nosadini, Birgit Rami, David Harris, Florian Hoellerl, Weier Qi, Zhihong Liu, Ryuji Iwatani, Y. Ogawa, Emi Kazuyama, Mirian A. Boim, Tetsuya Nagae, Kanako Matsubara, Qing Li, Giacomo Garibotto, Li-Min Xu, Kenji Ito, Xiao Ru Huang, Steven J. Harper, Giuseppe Remuzzi, Adelson Marçal Rodrigues, Jiaze Li, Hong-Lang Xie, Takashi Oite, Takuya Ishimura, Margarita Angerosa, Rodrigo Tambellini, Claude Granier, Liu Maodong, Yanling Zhang, Yong-Chun Ge, Ayako Fujimi, Dong Zheng, Yoshimi Takamiya, Mohamed H. Sayegh, Yu-Chi Cheng, Sara Conti, Qingxian Zhang, Hye Kyoung Song, Yu-Yan Fan, Jackson Souza-Menezes, Masashi Mukoyama, Hideki Yokoi, Xin Ming Chen, Ma. Mónica Elías, Zhai Shana, Melina A. Pagotto, Lorena Longaretti, Yonghong Shi, Assam El-Osta, Carol A. Pollock, Zanzhe Yu, Jad Kheir, Yoon Sik Chang, Takao Saito, Maha El Enazy, Laura Giardino, Ya Wang, Aneta Balcerczyk, Raquel C. Castiglione, Alessio Mocci, Sharma Prabhakar, Piotr Ksiazek, Ariela Benigni, Carla Zoja, Edith Schober, Shi Yonghong, Yan Sun, Duo Li, Wei-Wei Zhu, Yi-chun Tsai, Stephen I. Alexander, Seiya Okuda, Qi Cao, Edgar Maquigussa, Cheol Whee Park, Sara Molinas, Takako Mizutani, Kei Fukami, Yusuke Kaida, Paulo Roberto Santos, Jee Han, Lin Tang, Wakako Kawarazaki, Noriko Satoh, Alex Yuri S. Sato, Hui Y. Lan, Anabela Almeida, Zhang Yanling, Zhaohui Ni, David Barit, Susumu Kanzaki, Jiaqi Qian, Yukinori Tamura, Farid Nakhoul, David O. Bates, Fernando Dominici, Arthur C.K. Chung, Shirine Dada, Ying Li, Ji Hee Lim, Leyi Gu, Zhao-Hong Chen, Gastón Rojic, Sungjin Chung, Paolo Fiorina, Felipe M. Ornellas, Dae Cha, Roy Asaf, Huili Dai, Itaru Satoh, Laura Trumper, Masato Kasahara, Kazuwa Nakao, Jorge Giani, Carolina M.L. Barbosa, Oh Yeun Kwon, Atsushi Fukatsu, Jorge Toblli, Weiming Zhan, Alexander J. Szalai, Hoda Awad, Kiyomi Koike, Atsushi Hayashi, Shanyan Lin, Yucheng Yan, Fei Liu, Anna Watson, Sabine Peres, Jun Gao, Andrew H.J. Salmon, Faical Jarraya, Karin Jandeleit-Dahm, Monica Moreira-Rodrigues, Omar P. Pignataro, Gerit-Holger Schernthaner, Andrea Augello, Guntram Schernthaner, Giuseppe Garigali, Marcelo M. Morales, Michal Dragan, Hiroyuki Kobori, Monika Buraczynska, Grazyna Orlowska-Kowalik, Daniella Brasacchio, Janete Quelhas-Santos, Tatiana Maron-GutierrezGutierrez, Seiji Ueda, and Guo Quin Wang more...
- Subjects
Transplantation ,medicine.medical_specialty ,Nephrology ,business.industry ,Basic research ,Diabetes mellitus ,Medicine ,business ,Intensive care medicine ,medicine.disease - Published
- 2009
- Full Text
- View/download PDF
Catalog
3. Delivery of megsin siRNA plasmid reveals therapeutic potential against diabetic nephropathy by down-regulating p27(kip1) level.
- Author
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Liu M, Zhang Y, Chi Y, Zhai S, Wang B, Shi Y, and Li Y
- Subjects
- Animals, Blotting, Western, Cell Proliferation, Cells, Cultured, Collagen Type IV metabolism, Creatinine blood, Diabetes Mellitus, Experimental complications, Diabetes Mellitus, Experimental genetics, Diabetes Mellitus, Experimental metabolism, Diabetes Mellitus, Experimental pathology, Diabetic Nephropathies etiology, Diabetic Nephropathies genetics, Diabetic Nephropathies metabolism, Diabetic Nephropathies pathology, Down-Regulation, Male, Matrix Metalloproteinase 2 metabolism, Mesangial Cells pathology, Mice, Nephrectomy, Proteinuria etiology, Proteinuria genetics, Proteinuria metabolism, Proteinuria prevention & control, RNA, Small Interfering administration & dosage, Serpins metabolism, Time Factors, Tissue Inhibitor of Metalloproteinase-2 metabolism, Transfection, Cyclin-Dependent Kinase Inhibitor p27 metabolism, Diabetes Mellitus, Experimental therapy, Diabetic Nephropathies prevention & control, Genetic Therapy methods, Mesangial Cells metabolism, RNA Interference, RNA, Small Interfering metabolism, Serpins genetics
- Abstract
Background: Diabetic nephropathy is a complex disease with poor outcomes, and our current treatment measures are limited. It is urgent to search for novel therapeutic targets. Recently, a mesangium-predominant gene, megsin, has emerged as a participant in mesangial cell proliferation and/or mesangial matrix expansion. This study investigated the effect of megsin down-regulation on the progression of diabetic nephropathy., Methods: Streptozotocin (STZ)-induced diabetic CD-1 mice after uninephrectomy received a pBAsi mU6 Neo megsin siRNA plasmid for 12 weeks and were compared with age-matched nondiabetic mice. In vitro mouse mesangial cells were transfected with pBAsi mU6 Neo megsin siRNA plasmid using Lipofectamine 2000 reagent and further cultured in DMEM containing high glucose for up to 48 hours. All of the cells were collected for protein extraction and the supernatant for type IV collagen measurement. The expression of megsin, matrix metalloproteinase-2 (MMP-2), tissue inhibitor of metalloproteases-2 (TIMP-2) and p27(Kip1) was determined by Western blotting., Results: The megsin siRNA plasmid alleviated proteinuria and glomerular type IV collagen accumulation 12 weeks after the STZ injection, down-regulated renal cell proliferation and normalized the imbalance between MMP-2 and TIMP-2. Also, in vitro experiments showed that the glomerular mesangial cellular proliferation and type IV collagen production induced by high glucose were relieved after the transfection of megsin siRNA plasmid. The level of p27(kip1) was down-regulated in transfected mesangial cells significantly., Conclusions: The study suggests that the down-regulation of megsin might exert beneficial effects on the diabetic kidney partly through down-regulation of p27(kip1) level and that megsin may serve as a novel therapeutic target in the management of diabetic nephropathy. more...
- Published
- 2012
- Full Text
- View/download PDF
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