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Background: Hepatocellular carcinoma(HCC) cells-derived exosomal LncRNA SNHG16(LncSNHG16) is highly expressed and associated with the poor overall survival of patients. Telocytes(TCs), as novel interstitial cells, possess the function to promote HCC metastasis. Therefore, whether a molecular interaction between exosomal LncSNHG16 and TCs will be revealed in our study.Methods: LncSNHG16 expression in HCC tissues and cell lines were detected, and its bioinformatics analysis was screened. Exosomes were isolated and purified from HCC cells with over-expressed/knockdown LncSNHG16 vectors and co-cultured with TCs. Then, the expression of the LncSNHG16/miR-942-3p/MMP9 axis was tested in TCs. Transwell and cell wound healing assays were designed to examine the invasion and migration of HCC cells after co-incubating with TCs. RNA immunoprecipitation(RIP) assay and Dual-Luciferase gene report were utilized to verify the binding effect of LncSNHG16, miR-942-3p, and MMP9 mRNA. In vivo, experimental animal models were established to confirm the effect of exosomal LncSNHG16-induced MMP9 expression in HCC metastasis.Results: Exosomal LncSNHG16 could be phagocytized by TCs and down-regulated miR-942-3p, which induced the targeted MMP9 up-regulation. LncSNHG16 had special binding sites with miR-942-3p in TCs to facilitate the migration of HCC cells in vitro and in vivo. Exosomal LncSNHG16 was finally supported as competing endogenous RNA of the miR-942-3p/MMP9 axis in TCs.Conclusion: Tumor-derived exosomal LncSNHG16 modulates MMP9 by competitively binding to miR-942-3p in TCs, thus promoting the metastasis of HCC.

Objective: The objective of this study was to determine net energy (NE) of expeller-press (EP-RSM) and solvent-extracted rapeseed meal (SE-RSM) and to establish equations for predicting the NE in rapeseed meal (RSM) fed to growing pigs.Methods: Thirty-six barrows (initial body weight [BW], 41.1±2.2 kg) were allotted into 6 diets comprising a corn-soybean meal basal diet and 5 diets containing 19.50% RSM added at the expense of corn and soybean meal. The experiment had 6 periods and 6 replicate pigs per diet. During each period, the pigs were individually housed in metabolism crates for 16 days which included 7 days for adaption to diets. On day 8, pigs were transferred to respiration chambers and fed their respective diet at 2,000 kJ metabolizable energy (ME)/kg BW0.6/d. Feces and urine were collected, and daily heat production was measured from day 9 to 13. On days 14 and 15, the pigs were fed at 890 kJ ME/kg BW0.6/d and fasted on day 16 for evaluation of fasting heat production (FHP).Results: The FHP of pigs averaged 790 kJ/kg BW0.6/d and was not affected by the diet composition. The NE values were 10.80 and 8.45 MJ/kg DM for EP-RSM and SE-RSM, respectively. The NE value was positively correlated with gross energy (GE), digestible energy (DE), ME, and ether extract (EE). The best fit equation for NE of RSM was NE (MJ/kg DM) = 1.14×DE (MJ/kg DM)+0.46×crude protein (% of DM)–25.24 (n = 8, R2 = 0.96, p2 = 0.77, p = 0.018) may be utilized to quickly determine the NE in RSM when DE or ME values are unavailable.Conclusion: The NE values of EP-RSM and SE-RSM were 10.80 and 8.45 MJ/kg DM. The NE value of RSM can be well predicted based on energy content (GE, DE, and ME) and proximate analysis.

Objective: The objective of this study was to determine the digestible energy (DE) and metabolizable energy (ME) of yellow dent corn sourced from different meteorological origins fed to growing pigs and develop equations to predict the DE and ME of yellow dent corn from southwestern China.Methods: Sixty crossbred barrows were allotted to 20 treatments in a triplicate 20×2 incomplete Latin square design with 3 replicated pigs per dietary treatment during 2 consecutive periods. Each period lasted for 12 days, and total feces and urine during the last 5 days of each period were collected to calculate the energy contents.Results: On dry matter (DM) basis, the DE and ME in 20 corn grain samples ranged from 15.38 to 16.78 MJ/kg and from 14.93 to 16.16 MJ/kg, respectively. Selected best-fit prediction equations for DE and ME (MJ/kg DM basis) for yellow dent corn (n = 16) sourced from southwestern China were as follows: DE = 28.58–(0.12×% hemicellulose)+(0.35×% ether extract)–(0.83×MJ/kg gross energy)+(0.20×% crude protein)+(0.49×% ash); ME = 30.42– (0.11×% hemicellulose)+(0.31×% ether extract)–(0.81×MJ/kg gross energy).Conclusion: Our results indicated that the chemical compositions, but not the meteorological conditions or physical characteristics could explain the variation of energy contents in yellow dent corn sourced from southwestern China fed to growing pigs.

Additional void ratio and yield stress that can be easily determined by one-dimensional consolidation test are two representative parameters for the assessment of clayey soil structure. In this paper, a “structural parameter” that defined as the normalization of the integral of additional void ratio times effective vertical stress until yield stress was proposed to evaluate the disturbance induced by soil structure changes. By conducting oedometer tests on Hangzhou clay at various disturbed states, the compression index, yield stress, and structural parameter were obtained. The evolution of normalized additional void ratio during one-dimensional loading is presented to establish the soil structural indexing strategies for assessment of clay structure disturbances. Based on the laboratory investigations, a linear relationship between the normalized structural parameter and normalized yield additional void ratio was obtained. For a certain soil, specimens with the same initial void ratio but different disturbed state have a nearly constant void ratio when yielding. It is also found that soils with a higher plasticity index exhibit stronger structure and lower compressibility. In addition, quick direct shear tests were carried out to examine the undrained shear strength of soils at different disturbed states. The soil disturbance degree quantified from the undrained shear strength is found slightly higher than that from the structural parameter. This study can provide a reference for assessing the clayey soil structure.

This study was conducted to determine the digestible energy (DE) and metabolizable energy (ME) values and apparent total tract digestibility (ATTD) of nutrients in four fibrous ingredients [corn distillers’ dried grains with solubles (DDGS), soybean hull, wheat bran, and corn bran] fed to barrows at three different growth stages. Thirty growing barrows, 30 finishing barrows, and 30 fattening barrows (initial body weights of 29.04, 58.57, and 105.65 kg, respectively) were individually housed in metabolism crates and allotted to one of four test diets or a basal corn–soybean meal diet in a 3 × 5 factorial design. Fecal and urine samples were collected for 5 d after a 12 d adaption period. The DE and ME values and ATTD of gross energy (GE), organic matter (OM), crude protein, and acid detergent fiber (ADF) in wheat bran, as well as the ATTD of GE, OM, neutral detergent fiber (NDF), and ADF in corn DDGS, and ATTD of NDF and ADF in soybean hull, were greater (P

This study was conducted to determine the effect of different growing stages on digestible energy (DE) and metabolizable energy (ME) in soybean meal (SBM), cottonseed meal (CSM), rapeseed meal (RSM), corn gluten meal (CGM), and corn distillers dried grains with solubles (DDGS) fed to crossbred barrows. Thirty-six growing barrows (GS 1: 29.1 ± 2.85 kg BW), 36 growing barrows (GS 2: 59.2 ± 4.91 kg BW), and 36 finishing barrows (GS 2: 105.4 ± 6.91 kg BW) were allotted to 6 diets in a completely randomized design with 6 replicated pigs per diet per growth stage or BW. Faecal and urine samples were collected for 5 days after a 12-day adaption period. The results showed that the concentration of DE in corn for pigs at GS 3 was greater (P

The objectives of this experiment were to (a) determine the effects of fiber increase in diets on heat production (HP), (b) determine the net energy (NE) of oat bran (OB), wheat bran (WB), and palm kernel expellers (PKE) fed to growing pigs using indirect calorimetry (IC). Twenty-four growing barrows (29.2 ± 2.6 kg) were randomly allotted to one of four diets with six replicate pigs per diet. Diets included a corn-soybean meal basal diet and three test diets containing 30% OB, WB or PKE, respectively. During each period, pigs were individually housed in metabolism crates for 20 days, including 14 days to adapt to the diets. On day (d) 15, pigs were transferred to the open-circuit respiration chambers for determination of daily total HP and were fed one of the four diets at 2.3MJ ME/kg body weight (BW)0.6 /day. Total feces and urine were collected for the determination of digestible energy (DE) and metabolizable energy (ME) and daily total HP was measured from d 15 to d 19 and fasted on day 20 for the measurement of fasting heat production (FHP). The apparent total tract digestibility (ATTD) of dry matter (DM), gross energy (GE), and organic matter (OM) were greater (p

Helicobacter pylori (HP) infection is one of the most frequent bacterial infections in humans and is associated with the pathogenesis of gastric motility disorders such as delayed gastric emptying (DGE). Although HP infection is considered to delay gastric emptying, there has been little research on the underlying mechanism. Gastric motility involves interactions among gastrointestinal hormones, smooth muscle, enteric and extrinsic autonomic nerves and interstitial cells of Cajal (ICCs), and ICCs play an important role in gastrointestinal motility. Mutation or loss of stem cell factor (SCF) expression is known to reduce the number of ICCs or alter the integrity of the ICC network, contributing to gastrointestinal dysmotility. The aim of the present study was to investigate whether a reduction in ICCs contributes to the DGE caused by HP. A mouse model of HP infection was established and gastric emptying was compared between HP-infected and uninfected mice using the bead method. In addition, ICC counts and SCF expression levels in gastric tissue were evaluated using immunohistochemistry and western blotting, respectively. The results revealed that gastric emptying was significantly slower, the number of ICCs in gastric tissue was significantly reduced and the protein level of SCF in gastric tissue was significantly decreased in HP-infected mice compared with uninfected mice. Therefore, it may be concluded that HP reduced the number of ICCs by decreasing the expression of SCF protein in gastric tissue, thereby causing DGE.

Apart from energy balance trials such as calorimetry, growth trials could also be used to estimate the energy values of feed ingredients with caloric efficiency as an indicator. Recent work used such methods reported greater net energy (NE) value of soybean meal (SBM) relative to corn in nursery pigs. We theoretically compared the NE values of SBM and corn according to the definition of NE and properties of the major chemical compositions in each ingredient. Meanwhile, we thoroughly examined the diet formulations and related analysis used in this work and compared this study with some peer works. We found that this study may suffer from problems with experimental design, reference citation, and data interpretation. In summary, the conclusion from the recent work that the SBM NE value may be greater than the corn NE value is likely to be erroneous.

Hepatocellular carcinoma (HCC) is regarded as a leading cause of cancer-related deaths, and its progression is associated with hypoxia and the induction of hypoxia-inducible factor (HIF). Meloxicam, a selective cyclooxygenase-2 (COX-2) inhibitor, induces cell death in various malignancies. However, the underlying mechanism remains to be elucidated in HCC, especially under hypoxic conditions. The alteration of COX-2 and HIF-1α oncogenicity was evaluated in HCC specimens by tissue microarray. Cell viability, angiogenesis assays, and xenografted nude mice were used to evaluate the effects of meloxicam, along with flow cytometry to detect the cell cycle, apoptosis, and mitochondrial membrane potential (ΔΨm) of HCC. qRT-PCR, Western blotting, immunofluorescence, immunohistochemistry, luciferase assay, and RNAi were carried out to determine the HIF-1α signaling affected by meloxicam. In this study, we showed that meloxicam exerts antiproliferative and antiangiogenesis efficacy in vitro and in vivo and causes disruption of mitochondrial membrane potential (ΔΨm), thus leading to caspase-dependent apoptosis under hypoxic environments. Exposure to meloxicam significantly reduced HIF-1α transcriptional activation and expression through sequestering it in the cytoplasm and accelerating degradation via increasing the von Hippel-Lindau tumor suppressor protein (pVHL) in HCC. These data demonstrated that inhibition of HIF-1α by meloxicam could suppress angiogenesis and enhance apoptosis of HCC cells. This discovery highlights that COX-2 specific inhibitors may be a promising therapy in the treatment of HCC.

Dynamic fractures are a geological attribute of water flooding development in tight fractured oil reservoirs. However, previous studies have mainly focused on the opening mechanism of dynamic fractures and the influence of dynamic fractures on development. Few attempts have been made to investigate the optimization of the dynamic fracture parameter. In this study, the inverted square nine-spot well pattern model is established by taking fractured reservoir’s heterogeneity and its threshold pressure gradients into account. This simulation model optimizes the various parameters in a tight fractured oil reservoir with dynamic fractures, that is, the intersection angle between the dynamic fractures and the well array, the number of dynamic fractures, the penetration ratio, and the conductivity of the oil well’s hydraulic fractures. The results of this optimization are used to investigate the oil displacement mechanism of dynamic fractures and to discuss a mechanism to enhance oil recovery using an inverted square nine-spot well pattern. The simulation results indicate that a 45° intersection angle can effectively restrain the increase in the water cut. A single dynamic fracture can effectively control the displacement direction of the injected water and improve the oil displacement efficiency. Moreover, the optimal penetration ratio and the conductivity of the hydraulic fracture are 0.6 and 40 D-cm, respectively.

Variation laws of earth pressure accounting for the displacement of are taining wall can be well described by mathmatical fitting in the study of the relationship between earth pressure and retaining wall displacement. The common mathematical function expressions of earth pressure displacement of retaining wall can be divided into sinusoidal function model, exponential like function model, hyperbolic function model, fitting function and semi-numerical and semi-analytical model function, etc. The characteristics and shortcomings of the current expression of earth pressure displacement function are summarized. Then combined with the field test and model test, the applicability and characteristics of various mathematical functions in predicting the displacement of earth pressure with retaining structures are analyzed. The results show that when the displacement is small, the sinusoidal function model and the quasi-exponential function model are close to the measured results. When the displacement of retaining structure is large, the fitting results of hyperbolic model and semi-numerical and semi-analytical model are better. For the prediction of earth pressure displacement relationship in passive area, the buried depth has a great influence. And the error between the theoretical value and the actual value has a great influence on the fitting result of the model.

Background. RAB9, as a member of the Rab GTPase family, is required for the transport of the mannose-6-phosphate receptor (MPR) from late endosomes to trans-Golgi network (TGN). However, the role of RAB9A in tumors, including liver cancer, is still unknown. Methods. We used pcDNA3.1 plasmid to upregulate the expression of RAB9A in Hep3b cells and used specific shRNA to downregulate the expression of RAB9A in HepG2 cells. Biological functions of RAB9A were performed by CCK-8 assay, colony formation assay, apoptosis analysis, transwell assays, and wound healing assays. Finally, an in-depth mechanism study was performed by western blot. Results. RAB9A promoted the proliferation and clonality of Hep3b and HepG2 cells. RAB9A also inhibited apoptosis and the activation of mitochondrial apoptotic pathway. In addition, RAB9A promoted the invasion and migration of Hep3b and HepG2 cells. Importantly, RAB9A activated the AKT/mTOR signaling pathway in human liver cancer cells. A double-effect inhibitor (BEZ235) significantly hindered the effect of RAB9A overexpression on the proliferation and invasion of Hep3b cells. Conclusion. Our data suggest that RAB9A plays a carcinogenic role in human liver cancer progression partially through AKT signaling pathways, suggesting that RAB9A may serve as a potential therapeutic target for liver cancer therapy.

The present study was conducted to investigate the effects of feeding level and dietary supplementation with crystalline amino acids (AA) on the determination of energy contents in corn for growing pigs. A total of 36 barrows with an average initial body weight of 41.4 ± 2.0 kg were allocated across six treatments in a 2 × 3 factorial arrangement that included 2 feeding levels (2400 kJ ME/kg BW0.6 per day or ad libitum) and 3 dietary AA supplementation patterns (corn diet with no AA; corn + 3AA diet with addition of lysine, threonine and tryptophan; corn + 5AA diet with greater levels of crystalline lysine, threonine and tryptophan supplementation than those in corn + 3AA diet plus crystalline methionine and isoleucine supplementation). The experiment was conducted in 6 consecutive periods. During each period, 6 pigs were allotted to 1 of 6 treatments, and each treatment corresponded to 1 of 6 respiration chambers. In each period, pigs were individually housed in metabolism crates and fed their respective diets at two feeding levels for 13 d, including 7 d adaptation to the feed, metabolism crate and environmental conditions. On d 8, the pigs were transferred to respiration chambers. Total feces and urine samples were collected and heat production was measured from d 8 to 12. On the last day of each period, the pigs were fasted for 24 h and fasting heat production was subsequently measured. The apparent total tract digestibility (ATTD) of dry matter (DM), organic matter (OM) and gross energy (GE) in diets and corn were not affected by feeding level; however, increasing feeding level tended (P = 0.09) to decrease the ATTD of crude protein (CP). Nitrogen retention increased (P

This study was conducted to determine the digestible (DE) and metabolisable energy (ME) content and the coefficient of apparent total tract digestibility (CATTD) of gross energy (GE) in 4 cereal grains (corn, wheat, barley and sorghum) fed to barrows and to quantify the effect of body weight (BW) on the digestibility of the 4 cereal grains. Twenty-four weanling barrows (13.3 ± 0.8 BW, stage 1), 24 growing barrows (31.6 ± 1.5 BW, stage 2), 24 finishing barrows (56.7 ± 1.8 BW, stage 3) and 24 fattening barrows (111 ± 3.8 BW, stage 4) were individually housed in metabolic crates and allotted to 1 of 4 diets with 6 pigs fed each diet at each BW in a completely randomized design. The 4 diets consisted of 973.4 g/kg of a cereal grain and 26.6 g/kg of vitamins and minerals. Faeces and urine samples were collected for 5 d following a 10 d adaptation period. The DE and ME content and the CATTD of GE in corn and wheat were greater (P

The objective of this study was to compare the effect of source (corn DDGS, rice bran, or soybean) or form of oil (extracted or intact) on apparent total tract digestibility (ATTD) and true total tract digestibility (TTTD) of acid-hydrolyzed ether extract (AEE) and fatty acids. The study determined and compared the ATTD or TTTD of AEE and fatty acids in extracted corn oil, rice oil, and soybean oil with intact oil in corn DDGS, full-fat rice bran, and full-fat soybean. Seventy-eight barrows (initial BW = 47.2 ± 3.9 kg; Duroc × Landrace × Yorkshire) were allotted to 1 of 13 dietary treatments in a randomized complete block design with 6 barrows in each dietary treatment. The 13 experimental diets included 1 cornstarch-soybean meal basal diet (AEE, 0.56%) and 3 diets containing 6% extracted oils (corn oil, rice oil, and soybean oil) and 9 diets supplemented with 3 levels of corn DDGS (17%, 34%, and 51%), full-fat rice bran (14%, 28%, and 42%), and full-fat soybean (12%, 24%, and 36%). These diets provided about 2%, 4%, and 6% intact oil, respectively. The barrows were housed in individual metabolism crates and were fed the assigned test diets at 4% of initial BW per day. A 5-d total collection of feces followed a 7-d diet adaptation period. The ATTD of AEE were calculated for each diet. The endogenous flow of AEE associated with each ingredient and values for TTTD were calculated using regression methods. The ATTD of AEE were greater ( < 0.05) for extracted oil than for intact oil. Compared to extracted oil, intact oil had lower ( < 0.01) ATTD of palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), and linolenic acid (C18:3). However, a source by form interaction ( < 0.01) was observed for ATTD of C18:0, C18:2, and C18:3. The ATTD of C18:0, C18:2, and C18:3 in extracted corn and rice oil were greater compared with intact corn DDGS and rice oil ( < 0.05), while there was no significant difference for the ATTD of C18:0, C18:2, and C18:3 between extracted soybean oil and intact oil in full-fat soybean. In conclusion, lipids that are extracted have a greater digestibility compared with intact lipids, and this is especially true regarding saturated fatty acids. The ATTD of AEE in 2 forms of rice oil (intact oil and extracted oil) was less than the values in corn oil and soybean oil. The TTTD of AEE in corn DDGS and full-fat soybeans were greater than in full-fat rice bran.

The T7 peptide, an active fragment of full-length tumstatin [the non-collagenous 1 domain of the type IV collagen α3 chain, α3 (IV) NC1], has exhibited potential antitumor effects in several types of cancer cells. However, the mechanism underlying its action against human hepatocellular carcinoma (HCC) remains unclear. The present study aimed to investigate the role of autophagy in T7 peptide-induced cytotoxicity in HCC cells in vitro and in vivo. The results revealed that the T7 peptide significantly reduced cell viability and induced cell cycle arrest in HCC cells. The T7 peptide induced apoptosis in HCC cells through upregulation of Bax, Fas, and Fas ligand, and through upregulation of the anti-apoptotic protein Bcl-2. In addition, treatment with the T7 peptide induced protective autophagy in HCC cells. Blocking autophagy by 3-methyladenineor bafilomycin A1 enhanced T7 peptide-induced apoptosis. Furthermore, co-treatment with MK-2206 (an Akt specific inhibitor) or rapamycin (an inhibitor of mTOR) enhanced T7 peptide-induced autophagy, whereas co-treatment with insulin (an activator of the Akt/mTOR signaling pathway) alleviated T7 peptide-induced autophagy, which suggested that the T7 peptide may induce autophagy activation via inhibition of the Akt/mTOR signaling pathway. Taken together, the present results demonstrated that suppression of autophagy potentiated the cytotoxic effects of the T7 peptide, and suggested that the T7 peptide may serve as a potential alternative compound for HCC therapy.

Objective: Feed energy required for pigs is first prioritized to meet maintenance costs. Additional energy intake in excess of the energy requirement for maintenance is retained as protein and fat in the body, leading to weight gain. The objective of this study was to estimate the metabolizable energy requirements for maintenance (MEm) by regressing body weight (BW) gain against metabolizable energy intake (MEI) in growing pigs.[br/] Methods: Thirty-six growing pigs (26.3 +/- 1.7 kg) were allotted to 1 of 6 treatments with 6 replicates per treatment in a randomized complete block design. Treatments were 6 feeding levels which were calculated as 50%, 60%, 70%, 80%, 90%, or 100% of the estimated ad libitum MEI (2,400 kJ/kg BW0.60 d). All pigs were individually housed in metabolism crates for 30 d and weighed every 5 d. Moreover, each pig from each treatment was placed in the open-circuit respiration chambers to measure heat production (HP) and energy retained as protein (REp) and fat (REf) every 5 d. Serum biochemical parameters of pigs were analyzed at the end of the experiment.[br/] Results: The average daily gain (ADG) and HP as well as the REp and REf linearly increased with increasing feed intake (p< 0.010). beta-hydroxybutyrate concentration of serum tended to increase with increasing feed intake (p = 0.080). The regression equations of MEI on ADG were MEI, kJ/kg BW0.60 d = 1.88xADG, g/d+782 (R-2 = 0.86) and MEm was estimated at 782 kJ/kg BW0.60 d. Protein retention of growing pigs would be positive while REf would be negative at this feeding level via regression equations of REp and REf on MEI.[br/] Conclusion: The MEm was estimated at 782 kJ/kg BW0.60 d in current experiment. Furthermore, growing pigs will deposit protein and oxidize fat if provided feed at the estimated maintenance level.

The inevitable influence of large excavation in soft soils on nearby tunnels is of great concern in practice. In this paper, the influence of a nearby large excavation on existing metro tunnels of the Ningbo Metro Line 1 in sensitive soft soils is investigated and presented. Considerable displacement in the left tunnel closer to the excavation induced by the nearby excavation was revealed by field monitoring. Visible cracks and leakages were observed in left tunnel linings. Three dimensional numerical simulations are conducted to investigate the responses of the ground and left tunnel due to the adjacent excavation. The development of bending moment and displacement of the left tunnel during different construction stages of the nearby excavation is obtained. Then the interaction mechanism between the nearby excavation, surrounding soils and existing twin tunnels is investigated, which is of significance to the interpretation of the influence of the nearby excavation on the existing twin tunnels. Several protective measures for alleviating the influence of adjacent excavation on left tunnel are studied, including divided excavation, soil improvement and a cut-off wall. It is found that the left tunnel is influenced to varying degrees during different construction stages and the time effect is distinct for this large excavation in soft soils, which would be suggestive to engineers to pay more attention to the protection of adjacent tunnel during the crucial construction stages. The bending moment and displacement of the left tunnel is strongly related to the unloading effects and displacement of surrounding soils, which can be alleviated by means of proper improvement of excavation sequence. Comparatively, longitudinally divided excavation is more effective in protecting the left tunnel than soil improvement or a cut-off wall. This study is of certain reference value for protecting metro tunnels adjacent excavation in soft soils.

This experiment was conducted to determine the effect of variety and planting year on the nutritive values of corn fed to growing pigs. Four corn varieties examined in this experiment were planted in the same village located in Longhua County, Heibei Province, China, in 2012, 2013, and 2014, respectively. During each year, corn was hand-harvested in early October and sun dried to about 14% moisture content. Three batches of twenty-four barrows (33.27±4.30, 31.88±2.93, 34.21±3.81 kg body wight [BW] in 2012, 2013, and 2014, respectively) were used and allotted to a complete block design with 4 diets and 6 replicate pigs per diet. Pigs were individually placed in metabolic crates. The four experimental diets were formulated by mixing each variety of corn and vitamins and minerals, respectively. A five-day collection period followed a seven-day diet acclimation period. The results indicated that variety of corn significantly influenced the available energy content (digestible energy [DE] on dry matter basis, p

Two experiments were conducted to determine the digestible (DE) and metabolizable energy (ME) content as well as the apparent (AID) and standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in double-low rapeseed cakes (DLRSC) fed to growing pigs. In Exp. 1, 66 growing pigs (initial body weight (BW): 36.6 ± 4.1 kg) were randomly allotted to 1 of 11 diets (n = 6) including a corn-soybean meal basal diet and 10 DLRSC test diets. The basal diet was formulated using corn and soybean meal while the DLRSC test diets contained 192.2 g/kg DLRSC added at the expense of the corn, soybean meal and lysine. In Exp. 2, 9 growing barrows (initial BW: 29.7 ± 3.1 kg) were surgically fitted with a T-cannula in the distal ileum and allotted to a 6 × 9 Youden square design with 6 periods and 9 diets. The diets included a N-free diet containing 689 g/kg cornstarch and 200 g/kg sucrose and 8 test diets which were formulated to include 400 g/kg of one of the DLRSC samples. The chemical composition of the 10 DLRSC samples varied considerably especially for aNDF, ADF, calcium, phosphorus and total glucosinolates. The DLRSC samples had a similar AA composition (CV

This experiment was conducted to determine the digestible and metabolizable energy (DE and ME) content of lipid sources when fed to growing pigs and to establish prediction equations for determining the DE and ME content of the lipid sources based on their fatty acid composition. Eighty-four crossbred growing barrows (Duroc × Landrace × Yorkshire, weighing 39.4 ± 3.5 kg) were randomly allotted to 1 of 14 diets with 6 pigs per diet involving 13 lipid sources and a basal diet composed of corn and soybean meal according to a randomized complete block design. The lipid sources were substituted into the basal diet at a level of 10% at the expense of corn and soybean meal. The barrows were housed in individual metabolism crates to facilitate separate collection of feces and urine, and were fed the assigned test diets at 4% of initial body weight per day. A 5-d total collection of feces and urine followed a 7-d diet adaptation period. The results showed that the DE and ME content of lipid sources were affected by the composition of their fatty acids. The DE and ME content of lipid sources containing more polyunsaturated fatty acids (PUFA) were usually greater. The DE and ME content of the most examined vegetable and animal lipid sources can be predicted by the content of PUFA, oleic acid (C18:1) and stearic acid (C18:0). The best prediction equation is: DE = 34.15 + 0.07 × PUFA + 0.21 × C18:0 − 0.04 × C18:1 ( R 2 = 0.84, P R 2 = 0.85, P

This study was performed to investigate the influence of maternal and post-weaning rumen-protected folic acid (RPFA) addition on growth performance, total tract digestibility, rumen fermentation parameters and blood metabolites of lambs. Sixty-six Hu lambs (50 ± 4.3 days of age and 16.4 ± 3.84 kg of body weight [BW], mean ± SD) from 120 Hu ewes (24 ± 4.2 months of age and 44.6 ± 5.43 kg of BW) were selected based on litter size, sex, BW and maternal diets and then assigned to six groups using a randomized block experimental design in a 3 × 2 factorial arrangement. The first factor was supplementary level of RPFA in the maternal diets and the different treatments included 0 mg (M0F), 16 mg (M16 F) and 32 mg (M32 F) FA per kg dry matter (DM). The second factor was the RPFA addition level in the offspring diets with 0 mg [OC] and 4.0 mg [OF] FA per kg DM. Lambs were fed a total mixed ration with a roughage-to-concentrate ratio of 50:50 on a DM basis. Average daily gain (ADG) of pre-weaning lambs showed a quadratic increase in response to RPFA added in the maternal diets. After weaning, dry matter intake was not affected by treatments. The ADG increased and the feed conversion ratio (FCR) decreased by lamb RPFA addition. Total tract digestibility of DM, organic matter and crude protein was higher for OF than for OC, and total tract digestibility of ether extract and neutral detergent fibre increased with maternal RPFA addition. The ruminal pH decreased, whereas total volatile fatty acid levels increased with maternal RPFA addition. The acetate to propionate ratio was elevated with maternal and/or lamb RPFA addition. Activities of amylase, trypsin, chymotrypsin and lipase in the intestinal chyme also increased with maternal RPFA addition. Concentrations of blood glucose, albumin, total protein, very low-density lipoprotein (VLDL), folate, growth hormone and insulin-like growth factor-1 (IGF-1) were greater for OF than for OC. Similarly, blood glucose, high density lipoprotein cholesterol, VLDL, folate and IGF-1 were elevated with maternal RPFA addition. These results indicate that addition of RPFA to the maternal and/or lamb diets promoted growth performance of the lambs by improving ruminal fermentation, intestinal enzymes activity, total tract digestibility and hormone secretion.

This experiment was conducted to determine the effects of inclusion level of soybean oil (SO) and palm oil (PO) on their digestible and metabolism energy (DE and ME) contents when fed to growing pigs by difference and regression method. Sixty-six crossbred growing barrows (Duroc×Landrace×Yorkshire and weighing 38.1±2.4 kg) were randomly allotted to a 2×5 factorial arrangement involving 2 lipid sources (SO and PO), and 5 levels of lipid (2%, 4%, 6%, 8%, and 10%) as well as a basal diet composed of corn and soybean meal. The barrows were housed in individual metabolism crates to facilitate separate collection of feces and urine, and were fed the assigned test diets at 4% of initial body weight per day. A 5-d total collection of feces and urine followed a 7-d diet adaptation period. The results showed that the DE and ME contents of SO and PO determined by the difference method were not affected by inclusion level. The DE and ME determined by the regression method for SO were greater compared with the corresponding respective values for PO (DE: 37.07, ME: 36.79 MJ/kg for SO; DE: 34.11, ME: 33.84 MJ/kg for PO, respectively). These values were close to the DE and ME values determined by the difference method at the 10% inclusion level (DE: 37.31, ME: 36.83 MJ/kg for SO; DE: 34.62, ME: 33.47 MJ/kg for PO, respectively). A similar response for the apparent total tract digestibility of acid-hydrolyzed ether extract (AEE) in lipids was observed. The true total tract digestibility of AEE in SO was significantly (p