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10. Polysaccharides from Brasenia schreberi with Great Antioxidant Ability and the Potential Application in Yogurt.

Author

Wang, Yujie, Zou, Yue, Fang, Qiong, Feng, Ruizhang, Zhang, Jihong, Zhou, Wanhai, and Wei, Qin

Subjects
YOGURT, POLYSACCHARIDES, CELL culture, LACTIC acid bacteria, CAENORHABDITIS elegans, BIOACTIVE compounds, OXIDANT status
Abstract

Brasenia schreberi is a widely consumed aquatic plant, yet the knowledge regarding its bioactive components, particularly polysaccharides, remains limited. Therefore, this study aimed to optimize the extraction process of polysaccharides from B. schreberi using the response surface method (RSM). Additionally, we characterized the polysaccharides using various methods and assessed their antioxidant capabilities both in vitro and in vivo, employing cell cultures and Caenorhabditis elegans. Furthermore, these polysaccharides were incorporated into a unique yogurt formulation. Our findings demonstrated that hot water extraction was the most suitable method for extracting polysaccharides from B. schreberi, yielding samples with high sugar content, significant antioxidant capacity, and a well-defined spatial structure. Moreover, pectinase was employed for polysaccharide digestion, achieving an enzymolysis rate of 10.02% under optimized conditions using RSM. Notably, the results indicated that these polysaccharides could protect cells from oxidative stress by reducing apoptosis. Surprisingly, at a concentration of 250 μg/mL, the polysaccharides significantly increased the survival rate of C. elegans from 31.05% to 82.3%. Further qPCR results revealed that the polysaccharides protected C. elegans by up-regulating the daf-16 gene and down-regulating mTOR and insulin pathways, demonstrating remarkable antioxidant abilities. Upon addition to the yogurt, the polysaccharides significantly enhanced the water retention, viscosity, and viability of lactic acid bacteria. These outcomes underscore the potential of polysaccharides from B. schreberi as a valuable addition to novel yogurt formulations, thereby providing additional theoretical support for the utilization of B. schreberi. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Puerarin protects against acetaminophen‐induced oxidative damage in liver through activation of the Keap1/Nrf2 signaling pathway

Author

Zhou, Wanhai, primary, He, Heng, additional, Wei, Qin, additional, Che, Litao, additional, Zhao, Xin, additional, Liu, Wenwen, additional, Yan, Yue, additional, Hu, Lianqing, additional, Du, Yonghua, additional, Yin, Zhongqiong, additional, Shuai, Yongkang, additional, Yang, Li, additional, and Feng, Ruizhang, additional

Published
2023
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17. Activating sulfur oxidation reaction via six-electron redox mesocrystal NiS2 for sulfur-based aqueous batteries

Author

Yang, Zhoudong, primary, Wang, Boya, additional, Chen, Yongjin, additional, Zhou, Wanhai, additional, Li, Hongpeng, additional, Zhao, Ruizheng, additional, Li, Xinran, additional, Zhang, Tengsheng, additional, Bu, Fanxing, additional, Zhao, Zaiwang, additional, Li, Wei, additional, Chao, Dongliang, additional, and Zhao, Dongyuan, additional

Published
2022
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20. An energetic Sn metal aqueous battery

Author

Zhou, Wanhai, Ding, Shixiang, Zhao, Dongyuan, and Chao, Dongliang

Abstract

Despite a promising prospect, aqueous batteries (ABs) are facing challenges of limited energy density (30∼100 Wh/kg). Metal anodes offer prospectively high energy with large voltage and specific capacity. However, the development of current metal anodes (e.g., Zn, Cu, Mn, Al, Fe, etc.) are impeded due to inevitable dendrite formation, corrosion, and hydrogen evolution reaction. Recently in Jouleand Journal of the American Chemical Society, Meng, Chao, and their co-workers demonstrated novel paradigms of high-energy Sn-based aqueous batteries (SnABs), which show promising reversibility and durability of Sn metail anodes. Here, we present the development of Sn metal anodes and appraise critically the state-of-the-art strategies toward energetic SnABs.

Published
2023
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21. Evolution analysis of FRIZZY PANICLE (FZP) orthologs explored the mutations in DNA coding sequences in the grass family (Poaceae)

Author

Li, Jia, primary, Zhang, Litian, additional, G. Elbaiomy, Rania, additional, Chen, Lilan, additional, Wang, Zhenrong, additional, Jiao, Jie, additional, Zhu, Jiliang, additional, Zhou, Wanhai, additional, Chen, Bo, additional, Soaud, Salma A., additional, Abbas, Manzar, additional, Lin, Na, additional, and El-Sappah, Ahmed H., additional

Published
2022
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24. In Silico Functional Prediction and Expression Analysis of C2H2 Zinc-Finger Family Transcription Factor Revealed Regulatory Role of ZmZFP126 in Maize Growth

Author

Li, Jia, primary, Zhang, Litian, additional, Yuan, Yibing, additional, Wang, Qi, additional, Elbaiomy, Rania G., additional, Zhou, Wanhai, additional, Wu, Hui, additional, Soaud, Salma A., additional, Abbas, Manzar, additional, Chen, Bo, additional, Zhao, Deming, additional, and El-Sappah, Ahmed H., additional

Published
2021
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27. Transcriptome analysis of Cinnamomum longepaniculatum by high-throughput sequencing

Author

Kuan Yan, Rui-Zhang Feng, Fang Chen, Zhou Wanhai, and Qin Wei

Subjects
0106 biological sciences, 0301 basic medicine, Gene annotation, lcsh:Biotechnology, Breeding, Crop, Biology, 01 natural sciences, Applied Microbiology and Biotechnology, DNA sequencing, Transcriptome, 03 medical and health sciences, lcsh:TP248.13-248.65, KEGG, Transcriptomics, Secondary metabolism, Unigenes, lcsh:QH301-705.5, Gene, Genetics, Simple sequence repeats (SSR), Gene Annotation, Metabolic pathway, Gene Ontology, 030104 developmental biology, lcsh:Biology (General), Microsatellite, Volatile terpenoids, Transcription, Pathway, 010606 plant biology & botany, Biotechnology
Abstract

Normal 0 false false false ES-CL X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:8.0pt; mso-para-margin-left:0cm; line-height:107%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-ansi-language:ES-CL;} Background: Cinnamomum longepaniculatum is an important commercial crop and the main source of volatile terpenoids. The biosynthesis of key bioactive metabolites of C. longepaniculatum is not well understood due to the lack of available genomic and transcriptomic information. To address this issue, we performed transcriptome sequencing of C. longepaniculatum leaves to identify factors involved in terpenoid metabolite biosynthesis. Results: Transcriptome sequencing of C. longepaniculatum leaves generated over 56 million raw reads. The transcriptome was assembled using Trinity and yielded 82,061 unigenes with an average length of 879.43bp and N50 value of 1,387 bp. Furthermore, Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis indicated that our assembly is 91% complete. The unigenes were used to query the non-redundant database based on sequence similarity; 42,809 unigenes were homologous to known genes indifferent species, with an annotation rate of 42.87%. The transcript abundance and Gene Ontology analyses revealed that numerous unigenes were associated with metabolism, while others were annotated in functional categories including transcription, signal transduction, and secondary metabolism. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that 19,260 unigenes were involved in 385 metabolic pathways, with 233 unigenes found to be involved in terpenoid metabolism. Additionally, 23,463 simple sequence repeats were identified using the microsatellite identification tool. Conclusion: This is the first detailed transcriptome analysis of C. longepaniculatum . The findings provide insights into the molecular basis of terpenoid biosynthesis and a reference for future studies on the genetics and breeding of C. longepaniculatum . Normal 0 false false false ES-CL X-NONE X-NONE

Published
2017

30. Activating sulfur oxidation reaction via six-electron redox mesocrystal NiS 2 for sulfur-based aqueous batteries.

Author

Yang Z, Wang B, Chen Y, Zhou W, Li H, Zhao R, Li X, Zhang T, Bu F, Zhao Z, Li W, Chao D, and Zhao D

Abstract

Sulfur-based aqueous batteries (SABs) are deemed promising candidates for safe, low-cost, and high-capacity energy storage. However, despite their high theoretical capacity, achieving high reversible value remains a great challenge due to the thermodynamic and kinetics problems of elemental sulfur. Here, the reversible six-electron redox electrochemistry is constructed by activating the sulfur oxidation reaction (SOR) process of the elaborate mesocrystal NiS 2 (M-NiS 2 ). Through the unique 6e - solid-to-solid conversion mechanism, SOR efficiency can reach an unprecedented degree of ca. 96.0%. The SOR efficiency is further revealed to be closely associated with the kinetics feasibility and thermodynamic stability of the M-NiS 2 intermedium in the formation of elemental sulfur. Benefiting from the boosted SOR, compared with the bulk electrode, the M-NiS 2 electrode exhibits a high reversible capacity (1258 mAh g -1 ), ultrafast reaction kinetics (932 mAh g -1 at 12 A g -1 ), and long-term cyclability (2000 cycles at 20 A g -1 ). As a proof of concept, a new M-NiS 2 ‖Zn hybrid aqueous battery exhibits an output voltage of 1.60 V and an energy density of 722.4 Wh kg cath -1 , which opens a new opportunity for the development of high-energy aqueous batteries., (© The Author(s) 2022. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd.)

Published
2022
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