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Abstract [Objective] The diurnal variation characteristics of photosynthesis and chlorophyll fluorescence of greenhouse grape varieties in Hotan area were investigated, and their photosynthetic capacity was comprehensively evaluated, in order to provide reference for the introduction and cultivation management of greenhouse grapes in this area. [Methods] Six grape varieties were introduced into Hotan greenhouse as materials. The photosynthetic active radiation (PAR), relative chlorophyll content (SPAD), photosynthesis, and chlorophyll fluorescence parameters of each variety were analyzed, and the principal component analysis was used to comprehensively evaluate the ability of each grape variety. [Results] (1) The PAR levels of greenhouse in different positions were in the order: Before the shed>behind the shed>middle of the shed, while the overall performance in different trellis was in the order: On the trellis>middle of the trellis>under the trellis. (2) The SPAD values of grape leaves on different trellis were in the order: on the trellis>middle of the trellis>under the trellis, while the order from high to low among varieties: ‘Crimson Seedless’, ‘Summer Black’, ‘Xinyu’, ‘Hutai 8’, ‘Sunshine Muscat’, and ‘Queen Nina’. (3) The diurnal variation of Pn, Gs, and Tr of each variety showed a bimodal curve, and the diurnal variation of Ci showed a U-shaped and W-shaped variation. Fv/Fo and Fv/Fm generally showed a downward trend and then an upward trend. (4) The photosynthetic capacity of the six grape varieties was in the order: ‘Crimson Seedless’ >‘Summer Black’ >‘Sunshine Muscat’ >‘Xinyu’ > ‘Hutai 8’ >‘Queen Nina’. [Conclusion] ‘Crimson Seedless’ and ‘Summer Black’ have higher Pn, Gs, Tr, Fo, Fm and lower Fv/Fo and Fv/Fm, which are able to adapt to the Hotan environment of high temperature and high light intensity.

In Mexico, viticulture represents the second source of employment in the agricultural area after the fruit and vegetable sector. In developed countries, remote sensing is widely used for vineyard monitoring; however, this tool is barely used in the developing countries of Iberoamerica. In this research, our overall objective is to characterise two vineyards in the state of Queretaro (Mexico) using Sentinel-2 and meteorological data, specifically spectral and thermal indices. Results show that spectral indices obtained from Sentinel-2 bands have adequately characterised the phenological dynamics of the different varieties of the vineyards. The Modified Soil-Adjusted Vegetation Index (MSAVI) was adequately used to discriminate between the first stages of vineyards, while the Normalized Difference Vegetation Index (NDVI) was useful for monitoring vineyards during the rest stages of vineyards. Thermal indices have shown that the best grape varieties are those that can adapt to both cooler and warmer temperatures, have a reasonable ripening period, and can produce wines with balanced acidity and flavours. In conclusion, the combination of meteorological (including thermal indices) and remote sensing data (NDVI and MSAVI) provide information for choosing a suitable grape variety for this region. [ABSTRACT FROM AUTHOR]

The article presents the findings from a study evaluating the morphological, biological and technological characteristics of various table and technical grape varieties cultivated in the Republic of Azerbaijan. The research adhered to the descriptors of the International Organization of Vine and Wine (OIV). The study reveals a wide range in the size of grape bunches, with medium, large and small bunches being predominant. Notably, the local and introduced varieties Arna-grna, Khindogny, Hamashara, Shirvanshahy and Moldova exhibited superior morphological traits such as mass, volume and size of bunches. Juice yield, a key indicator of technological suitability, ranged from 45.5% to 80.0%. The Shiraz variety had the lowest juice yield (45.8%), whereas the Moldova (76.8%) and Uni Blanc (80.0%) varieties had the highest. Most varieties had small berries, but Arna-grna (481 g), Gyanjavi (341 g) and Moldova (317 g) were considered medium. The sugar content in the berries was categorized as average, high or very high, with Shirvanshahy having the highest sugar content (25.0 g/100 cm³). The titratable acidity of the varieties was low to average, while the active acidity was mainly low. [ABSTRACT FROM AUTHOR]

The monitoring of new grapevine varieties with superior agrobiological and technological characteristics, in relation to the evolution of the climatic factors, represents an important and continuous objective of the worldwide viticultural research and breeding programs. Observations and determinations of the current study were performed on 11 new table grapes varieties created in Romania, growing in the Ampelographic collection of the University of Life Sciences Iasi, north-eastern area of Romania. The grapevine varieties were evaluated under the morpho-structural aspect, regarding the leaf area, the average weight of the grapes, the number and weight of the berries, rachis weight, the number and weight of the seeds, the weight of the skin and pulp, calculating the main technological indices. From a biochemical point of view, the content of photosynthetic pigments in leaves, the concentration of soluble dry solids and titratable acidity of the grapes were determined in the climatic condition of the Copou - Iasi vineyard. Therefore, the monitoring of genetic resources provides useful data for grape producers and researchers regarding the integration of new table grape varieties into the viticultural ecosystems, evaluating their yield and quality in correlation with the evolution and influence of the climatic factors. [ABSTRACT FROM AUTHOR]

Grapes are a globally important fruit with significant economic value, influenced by factors such as sugar content, organic acids, hormones, and antioxidants. Understanding the dynamics of these compounds during grape development and ripening is critical for optimizing berry quality and production. This study investigates the changes in sugar, organic acids, hormones, and antioxidants in two grape varieties, 'Italia' and 'Bronx Seedless', at various growth stages (BBCH-77, BBCH-79, BBCH-81, BBCH-83, BBCH-85, and BBCH-89). Regarding sugars, significant variations were observed due to grapevine cultivar and phenological stage. 'Bronx Seedless' grapes consistently displayed lower sugar content than 'Italia' grapes, regardless of the type of sugar being examined. The BBCH-77 stage consistently exhibited lower sugar levels compared to BBCH-89. The varieties 'Bronx Seedless' and 'Italia' exhibited distinct nutritional profiles, each with their unique advantages in terms of sugar content and organic acid composition. Both varieties were rich in the primary sugar glucose and fructose, with 'Bronx Seedless' displaying notably high levels of the beneficial tartaric acid, enhancing its nutritional value. On the other hand, 'Italia' stood out for its higher concentrations of fumaric, butyric, and oxalic acids, contributing to its unique taste and health benefits. Throughout their growth stages from BBCH-77 to BBCH-89, an increase in organic acid levels was observed, peaking at the BBCH-85 stage, except for maleic acid. In terms of hormonal content, 'Italia' exhibited higher levels compared to 'Bronx Seedless'. The predominant hormone, abscisic acid (ABA), alongside lower quantities of zeatin, indicated a strong physiological response to environmental and developmental cues in both varieties, with hormone levels increasing as the grapes approached maturity. Antioxidant profiles also varied between the two varieties, with 'Italia' consistently showing higher antioxidant levels than 'Bronx Seedless'. Antioxidant levels consistently increased from BBCH-77 to BBCH-89. This comprehensive analysis contributes to our understanding of the complex processes underlying grape berry development and ripening, with potential implications for enhancing grape quality and refining production strategies. [ABSTRACT FROM AUTHOR]

Grapes are globally popular with wine production being one of the most well-known uses of grapes worldwide. Brazil has a growing wine industry, and the Serra Gaúcha region is a significant contributor to the country's wine production. Nonetheless, other states are increasing their relevance in this segment. Environmental factors and the soil microbiome (bacteria and fungi) heavily influence grape quality, shaping the crucial ''terroir'' for wines. Here, soil quality was assessed through nutrient analysis and bacteria microbial diversity, which could significantly impact grape health and final wine attributes. Soil samples from São Paulo's vineyards, focusing on Syrah, Malbec, and Cabernet Sauvignon, underwent chemical and microbial analysis via 16S rRNA metabarcoding and highlighted significant differences in soil composition between vineyards. Statistical analyses including PCA and CAP showcased region-based separation and intricate associations between microbiota, region, and grape variety. Correlation analysis pinpointed microbial genera linked to specific soil nutrients. Random Forest analysis identified abundant bacterial genera per grape variety and the Network analysis revealed varied co-occurrence patterns, with Cabernet Sauvignon exhibiting complex microbial interactions. This study unveils complex relationships between soil microbiota, nutrients, and diverse grape varieties in distinct vineyard regions. Understanding how these specific microorganisms are associated with grapes can improve vineyard management, grape quality, and wine production. It can also potentially optimize soil health, bolster grapevine resilience against pests and diseases, and contribute to the unique character of wines known as terroir.

The goal of this work is to assess the impact of grape varieties on the prices of Italian wines. As an important share of this wine is exported worldwide, we look at international markets. We gauge this influence by estimating a hedonic price model based on a sample of 2315 Italian still wines reviewed in Robert Parker’s Wine Advocate. The work expands results offered in literature so far as it considers quite a large number of international national and autochthonous varieties used for producing both red and white wines. Moreover, we propose an original perspective by exploring the different impacts of ageing on wine prices according to the different grape varieties utilized. Results show that, besides the well-known Italian geographical divide, many grape varieties significantly associate with different price levels. Overall, this impact is larger in the case of red wines than for the white ones. Furthermore, for the formers there are few well known varieties associated with positive price premiums, while for white wines, less widespread autochthonous varieties gain higher prices. Last, we found that successful ageing process involve both native varieties of northern and southern Italy as well as international ones. [ABSTRACT FROM AUTHOR]

Little is known about the relationship between distribution and market share in the wine category. Understanding the influences of product and distribution characteristics at the SKU-level and incorporating them into marketing strategy and planning has important managerial and academic implications. Sales of 3,524 wine SKUs across 4,218 stores in 4 states in the US over one year of observation are analyzed. We use an established distribution velocity model (Reibstein & Farris 1995) to estimate the relationship between distribution and market share. We then use the market share deviations from the expected values and apply a secondary robust regression to investigate possible relationships between various product- and distribution characteristics and those market share deviations. The results show that the distribution velocity in wine retailing is convex and increasing, in line with previous findings for other consumerpackaged goods in the marketing literature. Beyond distribution breadth, we find that overall parent brand performance (above), unit price (above), packaging type (above), country-of-origin, grape variety, sales consistency (above) and store specialization (below) are associated with above or below expected market share of wine SKUs. [ABSTRACT FROM AUTHOR]

In this study, the biology of grape mealybug, Planococcus ficus (Signoret, 1875) (Hemiptera: Pseudococcidae), was investigated in different grape varieties under laboratory conditions at 25 ± 1°C temperature, 65 ± 5% relative humidity, and 16:8 hours light:darkness photoperiod. Life tables of four different grapevine cultivars of Planococcus ficus were created, two of which are for wine (Emir, Kalecik karası) and two for table grapes (Yalova incisi, Trakya ilkeren). The results determined that the most suitable vine varieties for pest development were Emir and Yalova incisi, which are white grape varieties. The Yalova incisi and Emir cultivars were determined to be the most suitable host plants, with the highest values of life table parameters [generation time=31.6.4 ± 0.96 days, net reproductive rate = 32.3 ± 10.0 females/female/offspring, r=0.1 ± 0.01 females/female/day, gross reproductive rate=99.1 ± 16.99 nymphs/female] and (T0=38.4 ± 1.22 days, R0 = 40.8 ± 11.53 females/female/offspring, r = 9.7 ± 0.01 females/female/day, gross reproductive rate=155.1 ± 12.14 nymphs/female).

In Mexico, viticulture represents the second source of employment in the agricultural area after the fruit and vegetable sector. In developed countries, remote sensing is widely used for vineyard monitoring; however, this tool is barely used in the developing countries of Iberoamerica. In this research, our overall objective is to characterise two vineyards in the state of Queretaro (Mexico) using Sentinel-2 and meteorological data, specifically spectral and thermal indices. Results show that spectral indices obtained from Sentinel-2 bands have adequately characterised the phenological dynamics of the different varieties of the vineyards. The Modified Soil-Adjusted Vegetation Index (MSAVI) was adequately used to discriminate between the first stages of vineyards, while the Normalized Difference Vegetation Index (NDVI) was useful for monitoring vineyards during the rest stages of vineyards. Thermal indices have shown that the best grape varieties are those that can adapt to both cooler and warmer temperatures, have a reasonable ripening period, and can produce wines with balanced acidity and flavours. In conclusion, the combination of meteorological (including thermal indices) and remote sensing data (NDVI and MSAVI) provide information for choosing a suitable grape variety for this region.

The FTIR-ATR method coupled with the multivariate analysis of specific spectral areas of samples was developed to characterize two white grape varieties (Sauvignon Blanc and Hibernal) and two blue grape varieties (André and Cabernet Moravia) of wine planted and harvested in the Moravia region, Czech Republic. Principal component analysis and hierarchical cluster analysis were performed using fingerprint regions of FTIR spectra for all wines. The results obtained by principal component analysis in combination with linear discriminant analysis (PCA-LDA) scores yielded clear separation between the four classes of samples and showed very good discrimination between the wine samples, with a 91.7% overall classification rate for the samples. The conducted FTIR spectroscopy studies coupled with chemometrics allowed for the swift analysis of multiple wine components with minimal sample preparation. These methods can be used in research to improve specific properties of these wines, which will undoubtedly enhance the quality of the final wine samples obtained. [ABSTRACT FROM AUTHOR]

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Introduction: Climate change has been driving warming trends and changes in precipitation patterns and regimes throughout Europe. Future projections indicate a continuation of these trends in the next decades. This situation is challenging the sustainability of viniculture and, thus, significant efforts towards adaptation should be then carried out by local winegrowers. Method: Ecological Niche Models were built, using the ensemble modelling approach, to estimate the bioclimatic suitability of four main wine-producing European countries, namely France, Italy, Portugal, and Spain, in the recent past (1989–2005), for the cultivation of twelve Portuguese grape varieties. The models were then used to project the bioclimatic suitability to two future periods (2021– 2050 and 2051–2080) to better understand the potential shifts related to climate change (modeled after Intergovernmental Panel on Climate Change’s Representative Concentration Pathways 4.5 and 8.5 scenarios). The models were obtained with the modeling platform BIOMOD2, using four bioclimatic indices, namely the “Huglin Index”, the “Cool Night index”, the “Growing Season Precipitation index”, and the “Temperature Range during Ripening index” as predictor variables, as well as the current locations of the chosen grape varieties in Portugal. Results: All models performed with high statistical accuracy (AUC > 0.9) and were able to discriminate several suitable bioclimatic areas for the different grape varieties, in and around where they are currently located but also in other parts of the study area. The distribution of the bioclimatic suitability changed, however, when looking at future projections. For both climatic scenarios, projected bioclimatic suitability suffered a considerable shift to the north of Spain and France. In some cases, bioclimatic suitability also moved towards areas of higher elevation. Portugal and Italy barely retained any of the initially projected varietal areas. These shifts were mainly due to the overall rise in thermal accumulation and lower accumulated precipitation in the southern regions projected for the future. Conclusion: Ensemble models of Ecological Niche Models were shown to be valid tools for winegrowers who want to adapt to a changing climate. The long-term sustainability of viniculture in southern Europe will most likely have to go through a process of mitigation of the effects of increasing temperatures and decreasing precipitation. [ABSTRACT FROM AUTHOR]

Fruit cracking is a phenomenon in which the peel cracks during grape berry development, which seriously affects the yield and quality of the fruit. However, there are few studies on the mining of candidate genes related to berry cracking. In order to better understand the genetic basis of berry cracking, we used the results of previous quantitative trait locus (QTL) mapping, combined with field surveys of berry-cracking types and the berry-cracking rate, to mine candidate berry-cracking genes. The results showed that three identical QTL loci were detected in two years (2019 and 2020); and three candidate genes were annotated in the QTL interval. In mature berries, the expressions of the candidate genes were more abundant in the cracking-susceptible parent ('Crimson Seedless') than in the cracking-resistant parent ('Muscat Hamburg'). Grape berry cracking is a complex trait controlled by multiple genes, mainly including genes encoding cellulose synthaseelike protein H1, glucan endo-1,3-beta-glucosidase 12, and brassinosteroid insensitive 1-associated receptor kinase 1. The high expression of the candidate berry-cracking genes may promote the occurrence of berry cracking. This study helps elucidate the genetic mechanism of grape berry cracking. [ABSTRACT FROM AUTHOR]

Xinjiang is the largest grape-producing region in China and the main grape cultivation area in the world. The Eurasian grape resources grown in Xinjiang are very rich in diversity. The sugar composition and content are the main factors that determine the quality of berries. However, there are currently no systematic reports on the types and contents of sugars in grapes grown in Xinjiang region. In this research, we evaluated the appearance and fruit maturity indicators of 18 grape varieties during fruit ripening and determined their sugar content using GC-MS. All cultivars primarily contained glucose, D-fructose, and sucrose. The glucose content in varieties varied from 42.13% to 46.80% of the total sugar, whereas the fructose and sucrose contents varied from 42.68% to 50.95% and 6.17% to 12.69%, respectively. The content of trace sugar identified in grape varieties varied from 0.6 to 2.3 mg/g. The comprehensive assessment by principal component analysis revealed strong positive correlations between some sugar components. A comprehensive study on the content and types of sugar will provide the foundation to determine the quality of grape cultivars and effective ways to utilize resources to improve sugar content through breeding. [ABSTRACT FROM AUTHOR]

Grapes are a globally important fruit with significant economic value, influenced by factors such as sugar content, organic acids, hormones, and antioxidants. Understanding the dynamics of these compounds during grape development and ripening is critical for optimizing berry quality and production. This study investigates the changes in sugar, organic acids, hormones, and antioxidants in two grape varieties, ‘Italia’ and ‘Bronx Seedless’, at various growth stages (BBCH-77, BBCH-79, BBCH-81, BBCH-83, BBCH-85, and BBCH-89). Regarding sugars, significant variations were observed due to grapevine cultivar and phenological stage. ‘Bronx Seedless’ grapes consistently displayed lower sugar content than ‘Italia’ grapes, regardless of the type of sugar being examined. The BBCH-77 stage consistently exhibited lower sugar levels compared to BBCH-89. The varieties ‘Bronx Seedless’ and ‘Italia’ exhibited distinct nutritional profiles, each with their unique advantages in terms of sugar content and organic acid composition. Both varieties were rich in the primary sugar glucose and fructose, with ‘Bronx Seedless’ displaying notably high levels of the beneficial tartaric acid, enhancing its nutritional value. On the other hand, ‘Italia’ stood out for its higher concentrations of fumaric, butyric, and oxalic acids, contributing to its unique taste and health benefits. Throughout their growth stages from BBCH-77 to BBCH-89, an increase in organic acid levels was observed, peaking at the BBCH-85 stage, except for maleic acid. In terms of hormonal content, ‘Italia’ exhibited higher levels compared to ‘Bronx Seedless’. The predominant hormone, abscisic acid (ABA), alongside lower quantities of zeatin, indicated a strong physiological response to environmental and developmental cues in both varieties, with hormone levels increasing as the grapes approached maturity. Antioxidant profiles also varied between the two varieties, with ‘Italia’ consistently showing higher antioxidant levels than ‘Bronx Seedless’. Antioxidant levels consistently increased from BBCH-77 to BBCH-89. This comprehensive analysis contributes to our understanding of the complex processes underlying grape berry development and ripening, with potential implications for enhancing grape quality and refining production strategies.

The rootstock had a significant impact on the quantity and quality of grapes of the varieties grafted on it. That had been determined by the different growth strength of the rootstocks, their different absorption capacity and compatibility with the cultivated vine. During the period 2017-2020, a study was carried out on the agrobiological and technological properties of grapes and wine of the Kaylashki Rubin variety, grafted on vine rootstocks SO4, 110 Rihter, 44-53 Malegue and Fercal. Differences in the investigated indicators were found as a result of the influence of the used rootstocks. A mechanical analysis was made to determine the composition and structure of the clusters and berries. For the period, the highest average yield per vine was reported for Fercal rootstock, while the best theoretical yield had the variant on 44-53 Malegue rootstock. The chemical composition of grapes and experimental wines had been determined. On the average for the period the highest sugar accumulation and respectively the lowest titratable acids in grapes were reported in the variant of 44-53 Malegue rootstock. The opposite relationship was observed for 110 Richter. Differences in the chemical composition of the wines depending on the used rootstocks were found. The wines from 44-53 Malegue variant had the highest average alcohol content and sugar-free extract, but the lowest rates of titratable acids. The average amounts of the total phenolic compounds and anthocyanins in the wines from the variants of the different rootstocks were close. Their values were the lowest in the samples 2017 vintage. The rootstock type also affected the organoleptic properties of the Kaylashki Rubin wines; however, there was no common trend for the studied period. In 2018 and 2020, the samples from the 110 Rihter variant showed the best tasting qualities. [ABSTRACT FROM AUTHOR]

On January 1, 2023, a "reformed" Common Agricultural Policy (CAP) will come into force, which is innovative by nature in structural terms, and focused on environmental and social sustainability issues, aimed at a comprehensive digitizationbased modernization of the agri-food sector. The new CAP keeps the current structure based on expenditure and regulatory measures, but includes a new planning tool, the national CAP Strategic Plan, a new CAP management model, and the new delivery model (NDM). Concerning EU wine policy, the new regulations foresee a number of specific amendments to existing rules, including changes that will apply to financial support for the wine sector with a reduced budget and to the regulatory measures. Among the latter, the most globally impacting are labelling rules, which require more information to consumers and allow the use of an e-label, the use of hybrid grape varieties for the production of appellation wines, and the inclusion among CAP regulated products of partially or totally de-alcoholised wines. [ABSTRACT FROM AUTHOR]

IntroductionClimate change has been driving warming trends and changes in precipitation patterns and regimes throughout Europe. Future projections indicate a continuation of these trends in the next decades. This situation is challenging the sustainability of viniculture and, thus, significant efforts towards adaptation should be then carried out by local winegrowers.MethodEcological Niche Models were built, using the ensemble modelling approach, to estimate the bioclimatic suitability of four main wine-producing European countries, namely France, Italy, Portugal, and Spain, in the recent past (1989–2005), for the cultivation of twelve Portuguese grape varieties. The models were then used to project the bioclimatic suitability to two future periods (2021– 2050 and 2051–2080) to better understand the potential shifts related to climate change (modeled after Intergovernmental Panel on Climate Change’s Representative Concentration Pathways 4.5 and 8.5 scenarios). The models were obtained with the modeling platform BIOMOD2, using four bioclimatic indices, namely the “Huglin Index”, the “Cool Night index”, the “Growing Season Precipitation index”, and the “Temperature Range during Ripening index” as predictor variables, as well as the current locations of the chosen grape varieties in Portugal.ResultsAll models performed with high statistical accuracy (AUC > 0.9) and were able to discriminate several suitable bioclimatic areas for the different grape varieties, in and around where they are currently located but also in other parts of the study area. The distribution of the bioclimatic suitability changed, however, when looking at future projections. For both climatic scenarios, projected bioclimatic suitability suffered a considerable shift to the north of Spain and France. In some cases, bioclimatic suitability also moved towards areas of higher elevation. Portugal and Italy barely retained any of the initially projected varietal areas. These shifts were mainly due to the overall rise in thermal accumulation and lower accumulated precipitation in the southern regions projected for the future.ConclusionEnsemble models of Ecological Niche Models were shown to be valid tools for winegrowers who want to adapt to a changing climate. The long-term sustainability of viniculture in southern Europe will most likely have to go through a process of mitigation of the effects of increasing temperatures and decreasing precipitation.

The FTIR-ATR method coupled with the multivariate analysis of specific spectral areas of samples was developed to characterize two white grape varieties (Sauvignon Blanc and Hibernal) and two blue grape varieties (André and Cabernet Moravia) of wine planted and harvested in the Moravia region, Czech Republic. Principal component analysis and hierarchical cluster analysis were performed using fingerprint regions of FTIR spectra for all wines. The results obtained by principal component analysis in combination with linear discriminant analysis (PCA-LDA) scores yielded clear separation between the four classes of samples and showed very good discrimination between the wine samples, with a 91.7% overall classification rate for the samples. The conducted FTIR spectroscopy studies coupled with chemometrics allowed for the swift analysis of multiple wine components with minimal sample preparation. These methods can be used in research to improve specific properties of these wines, which will undoubtedly enhance the quality of the final wine samples obtained.

Grape canes represent a valuable source of numerous polyphenols with antioxidant properties, whose compositions vary depending on the genotype and environmental factors. Antioxidant activities of pure molecules are often reported without considering possible interactions that may occur in complex polyphenol mixture. Using UPLC-MS-based metabolomics and unsupervised classification, we explored the polyphenol variations in grape cane extracts from a collection of European varieties. Antioxidant activities were assessed using ORAC, ABTS, DPPH, FRAP, CUPRAC and chelation assays. Pairwise correlations between polyphenols and antioxidant capacities were performed to identify molecules that contributed more to the antioxidant capacities within a complex mixture of polyphenols. [ABSTRACT FROM AUTHOR]

The article examines the geographical area delimited for the production of the Divine and the specific characteristics and physical-geographical conditions of the Călărași vineyard through the prism of different methods of distillation and production of wine distillates. Technological regimes for distilling wine raw materials and organoleptic and physico-chemical indices such as: alcohol content, optical density, pH, aldehyde content, higher alcohol content and volatile acid content are scientifically argued. The quality indicators were determined for both wines and distillates obtained at the 'Charente' batch plant and the 'VAND-M-0' plant. The impact of the distillation method on the quality and production process of the wine distillate is evaluated.

This study analyses the differences between wines obtained from grapes cultivated conventionally and those cultivated biodynamically. The wine samples studied were obtained from Rhein Riesling, Italian Riesling, Muscat Ottonel and Chardonnay grape varieties. Among these, four variants (one from each grape variety) were obtained from grapes following conventional cultural technologies, while the rest were from the same grape varieties cultivated biodynamically. All grapes and wines were produced in the Murfatlar vineyard, in south-east Romania. Basic chemical and sensorial analyses were applied to evaluate the differences appearing between the conventional and organic wine samples. All sets of data, PCA analysed, underlined that there are no systematic differences between the two grape cultivation methods and the obtained products. [ABSTRACT FROM AUTHOR]

Grapevine is one of the major horticultural crops of the world with the cultivated area exceeding 7.5 million ha used for a myriad of products ranging through fresh table grape, preserves, juice, wine, and raisins. The main objective of this study was to introduce twenty-eight grapevine cultivars (ten wild, ten wine, four table, and four raisin grapes) into Gedeo Zone for the first time and ampelographically characterize them in Dilla and Yirgacheffe agroecological conditions in Gedeo Zone, Southern Ethiopia, from August 2018 to July 2021. Ten Vitis abyssinica wild grapevine cultivars were collected from Adama, Addis Ababa, Alamata, Arba Minch, Bahir Dar, Dire Dawa, Gondar, Hawassa, Jimma, and Jinka areas. Additional ten world class wine grapes were gathered from Ziway Castel Winery, and four table and four raisin grapes were also collected from Raya Horti Farm and Koka Vineyard at the same time. The experiment was a 2 × 28 factorial arranged in randomized complete block design (RCBD) with three replications, and data were analyzed using the R-software. The analysis of variance revealed that the interaction of cultivar and location significantly (P < 0.001) affected grapevine plant height, leaf number, number of fruits per plant, and tendril number per vine, while grapevine trunk diameter, flower cluster, root length, and number of suckers per vines were not significantly (P > 0.05) influenced by the interaction of the two factors. Generally, the wine grapevine cultivars had lower canopy such as plant height, leaf number, number of tendrils, and suckering vines while these registered a higher number of fruits per plant, trunk diameter, flower cluster, and root length compared to the wild grapevine cultivars. The results of the present study suggested that Syrah, Chenin Blanc, and Grenache can produce high grapevine berry yield and wine quality in Gedeo Zone agroecology particularly in Dilla location. The wild grapevines collected from Dire Dawa, Arba Minch, Jinka, and Alamata were the potential candidates for the world class wine, raisin, and table grapevines which could open new frontiers in the future for Ethiopian native Vitis abyssinica wild grapevine breeding and genetic engineering that will help to move the national and international viticulture and enology industry forward. As the Ethiopian native grapevines are at the risk of total extinction, adequate conservation strategies are required. Breeding, detailed identification, and introducing the potential grapes in different regions of the country are needed. This finding represents a step forward in efforts to understand hybridization of Vitis abyssinica grapevine with Vitis vinifera and other new world Vitis species. [ABSTRACT FROM AUTHOR]

Objective: Characterize the parameters that determine quality of wines variety Tempranillo made with berries grown in three geographical zones of Chihuahua, Mexico. Metodology: Determination of wine quality from 12 month berry maturity grown in three vineyards: Faciatec, Hacienda Bustillos and Paralelo meridian. Physicochemical analysis, total titratable acidity (ATT), total polyphenol index and color composition were determined in a complete block design with three replicates. Results: The values of pH and ATT were optimal for winemaking. However, volatile acidity was low, the wine with the highest alcoholic content and phenolic composition was vinified with the berries from Faciatec vineyard, it is also suitable for aging. Besides, its highest hue value was due to the chromatic and sensory characteristics. Limitations: Environmental conditions during the growing season and lack of surveillance to people coming inside the vineyard. Conclusions: Height above sea level, temperature and precipitation during maturity influence winemaking because they impact in chromatic and phenolic characteristics of the wine. Therefore, the wine from Faciatec vineyard is the most suitable for aging in barrels and the others for young wines. [ABSTRACT FROM AUTHOR]

Consumer interest in innovative wines has increased in recent years in Romania, in correlation with the development of the wine sector. Nowadays, the winemaking technique proposed by Dom Perignon (blanc de noirs) with applicability to sparkling wines is increasingly used to obtain still wines. This research aimed to identify the most suitable technology for obtaining white wines from Fetească neagră and Busuioacă de Bohotin grapes. The resulting wines were analysed from physicalchemical and chromatic points of view, and their sensory properties and volatile compounds content were also registered. The obtained samples had characteristics usually found in wines obtained from white grape varieties. Although frequently used in the production of base wine for sparkling wines, this technique can give original results in obtaining still wines as well. The use of activated carbon has led to the production of wines with a higher commercial value (improved colour characteristics). Given that red wines are significantly less consumed compared to white ones, this technique allows the use of black grape varieties and the production of innovative white wines. [ABSTRACT FROM AUTHOR]

The aim of this work was to analyze and compare the concentration of higher alcohols, esters, and acids in wines from Tempranillo and Tempranillo Blanco. Tempranillo Blanco is a new and little-studied white variety that originated from Tempranillo by a natural mutation. During three seasons, grapevines of both varieties were harvested, and nine wines were made from each. The volatile composition of the wines was determined by GC-MS. In the wines of both varieties, the content of higher alcohols was higher than those of esters and acids. Wines from Tempranillo Blanco had lower content of 2-phenylethanol, methionol, 1-hexanol, benzyl alcohol, and total higher alcohols, but higher hexyl acetate and ethyl decanoate than Tempranillo wines. Total ethyl esters and total esters were higher in Tempranillo wines due to the higher ethyl lactate and ethyl succinate content derivate from the malolactic fermentation that was not made in Tempranillo Blanco. The content of hexanoic and octanoic acids and total acids was also higher in Tempranillo Blanco wines than in Tempranillo. This is one of the first studies carried out on the wine volatile composition of Tempranillo Blanco and therefore contributes to a better understanding of the oenological characteristics of this white variety. [ABSTRACT FROM AUTHOR]

New Plant Breeding Techniques (NPBTs) protocols have been developed to produce new grape varieties with improved quantitative and qualitative characteristics. Reliable transformation protocols for grapes are based on the generation/induction of embryogenic callus cells that are then transformed. Varieties such as Italia have proven to be very recalcitrant to regeneration via somatic embryogenesis. In this work, the development of a protocol for improved production of embryogenic calluses is described. Two sterilization protocols were tested: (a) a lower active chlorine concentration for a longer time (LS); and (b) a higher chlorine concentration for a shorter time (HS), in combination with the absence or presence of citric acid in the growing substrate in the first growth media. The embryogenic calluses formation in Chardonnay, a cv. with a high embryogenic response, was significantly higher in presence of citric acid in the initial growing substrate regardless of the sterilization protocol. In Aglianico, a cv. with a lower embryogenic response, no significant differences were observed. Instead, in a recalcitrant cv. as Italia, we obtained a 13-fold increase in embryogenic calluses formation performing sterilization of flowers with the HS protocol compared to LS. [ABSTRACT FROM AUTHOR]

The paper investigates the effect of the enzymatic pre-treatment of grape seeds from six Romanian cultivars on the oil extracted. The grape seeds of some white and red Romanian grape varieties were separated from winery waste, washed, dried and ground, with the oil then obtained by extraction with petroleum ether. The extraction was performed directly or after a preliminary treatment with a commercial pectin lyase. The enzymatic procedure applied was more cost effective compared to other treatments previously described in which a cocktail of enzymes was used. The quantity of the extracted oil was measured in both types of processing, with an increase being observed for pre-treated samples. The fatty acid profiles (FAPs) of the oils resulted for the treated and untreated seeds were determined. No change in the composition was noticed. The reductive power of these oils was also investigated. Compared to the untreated samples for the same variety, the enzyme pre-treatment resulted in a superior antioxidant capacity. [ABSTRACT FROM AUTHOR]

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Making raisins (dried grapes) is a laborious process and grape growers around the world are increasingly interested in adopting mechanized methods, especially dry-on-vine (DOV). The DOV method entails severing canes bearing mature fruit, causing them to DOV, after which they may be harvested by machine. The overhead arbor is an important DOV raisin trellis system, but basic agronomic performance of raisin varieties on that system are scant. Therefore, a three-year experiment was conducted to compare the performance of several raisin grape varieties (Vitis vinifera L.), on an overhead arbor trellis, for production of DOV raisins. Grapevine variety, ‘DOVine’, ‘Fiesta’, ‘Selma Pete’, or ‘Thompson Seedless’, was the main-plot factor, and number of canes, six or eight, was the sub-plot. ‘Fiesta’ produced the highest yields, averaging about 12.24 Mg/ha raisins. However, ‘Fiesta’ generally had the lowest soluble solids and among the lowest raisin grades. The later ripening of ‘Fiesta’ fruit delayed harvest pruning to initiate fruit drying compared to the other varieties. Therefore, its raisins did not dry sufficiently to meet industry standards. ‘Selma Pete’, ‘DOVine’, and ‘Thompson Seedless’ had similar yields, generally about 20% less than ‘Fiesta’, but ‘Selma Pete’ produced fruit that were the largest in size, with the highest soluble solids, and that produced raisins of the highest grades, compared to the other varieties. Vines pruned to eight canes produced higher yields without sacrificing raisin quality. In conclusion, ‘Fiesta’ was notable for its high productivity, and ‘Selma Pete’ for its earliness and high quality, and both varieties had sufficient capacity to carry eight 15-node canes. [ABSTRACT FROM AUTHOR]

Grape production has a long history in the Elazig province and surrounding vicinity and produced grapes have been used for table consumption and also processed into traditional beverages, ¸Sıra (special non-alcoholic grape juice) and wine. In the Elazig province, the main grape cultivars are ‘A˘gın Beyazı’, ‘Öküzgözü’, ‘Bo˘gazkere’, ‘¸Silfoni’, ‘Tahannebi’, and ‘Köhnü’. Among them, ‘Köhnü’ cultivar is highly preferred by consumers due to its black color and perfect berry characteristics. The cultivar has grown for centuries in different parts of Elazig and shows a great variability for most of its morphological and biochemical characteristics. In the present study, we aimed to determine morphological and biochemical traits in six ‘Köhnü’ clones sampled from Elazı ˘ g. The cluster weight of six clones was found between 334–394 g. The highest total phenolic content was observed in seeds followed by peel and pulp samples. The seed extract of Clone 2 had the highest total phenolic content at 254 mg gallic acid equivalent/100 g fresh weight. The results also showed that peel, pulp, and seed samples of ‘Köhnü’ grape clones had considerable amounts of antioxidant components determined by DPPH (1,1-Diphenyl-2-picryl-hydrazyl), FRAP (ferric reducing antioxidant power), and TEAC assays and might be rich sources of natural antioxidants. Among the six ‘Köhnü’ clones, Clone 3, and Clone 6 differed from the others in respect to the highest cluster weight, the highest concentrations of total phenolic content, and antioxidant activity. The results also implied that all clones could be used potentially as a readily accessible source of natural antioxidants and as a possible pharmaceutical supplement. [ABSTRACT FROM AUTHOR]

The growing demand in recent years for sustainable wine production has led to the management of waste and by-products. Among them, vine-shoots could be used as additives comparable to the oak fragments widely employed in enology. This work analyzes the feasibility of applying vine-shoot chips during winemaking and the aging of Malbec and Bonarda wines from Mendoza (Argentina) and evaluates their chemical and sensory impact. Toasted (CHT) and untoasted (CHWT) vine-shoot chips obtained from a Bonarda vineyard were added in Malbec and Bonarda grapes during winemaking (Experiment A): C, control; CHWT, 12 g/L; CHT, 12 g/L. Furthermore, the same treatments were applied during aging (1M, 30 days; 2M, 60 days; 4M, 120 days) to the finished wines under controlled conditions (Experiment B). The impact of vine-shoot chips during winemaking was different between varieties. For Malbec alone, CHT caused a significant decrease in tannins, anthocyanins, and polymeric pigments, and a modification of wine color. During aging, CHWT and CHT had an impact mainly at the sensory level, increasing the wine’s complexity in terms of aromatic attributes and mouthfeel. In conclusion, the proposed technology could be a simple and economical tool for red wine production of high chemical and organoleptic quality. [ABSTRACT FROM AUTHOR]

The quality of wines has often been associated with their geographical area of production, as well as the grape variety used in their elaboration. Many research studies have been carried out to characterize and differentiate between red wines labeled with Protected Designation of Origin (PDO) from different geographical areas, but very few have been carried out on white and rosé wines. The objective of this work was to characterize white and rosé PDO wines from different geographical areas of Spain very close to each other elaborated with different grape varieties and select the variables that most contribute to their differentiation. Analysis of variance (ANOVA) and principal component analysis (PCA) were used as statistical methods. The ethanol content was the nonvolatile variable that most contributed to differentiating between some of the white and rosé wines according to their PDO. The white wines from RD (Ribera del Duero) and BI (Bierzo) were characterized by a high terpenic content (floral notes) while the wines from RU (Rueda), TO (Toro) and CI (Cigales)by a high content of ethyl esters and alcohol acetates (fruity aromas). The rosé wines elaborated with the Mencía grape variety from BI were characterized by their highest polysaccharidic content, which could have a positive sensory effect on the mouthfeel. The rosé wines from CI were characterized by their volatile profile complexity, having the highest content of volatile compounds from the oak wood, terpenes and C6 alcohols which provide pleasant woody, floral and herbaceous aromas. On the contrary, the RD wines were richest in alcohol acetates responsible for fruity aromas. [ABSTRACT FROM AUTHOR]

(1) Background: Currently, some ampelographic methods are developing in order to identify grapevine varieties. For this purpose, morpho-colorimetric parameters in leaves have been analyzed by digital imagen analysis, but some environmental conditions may affect their determinations. (2) Methods: A research study was conducted to characterize leaf morpho-colorimetric parameters in five grapevine varieties growing under different plant water status and to discriminate them under these conditions. Leaves were collected in vines, and twelve leaf morpho-colorimetric and fractal dimension variables were assessed. (3) Results: Merlot presented the highest values of perimeter and area to perimeter ratio in leaves and higher leaf area than Chardonnay in both plant water conditions. Most of the leaf morpho-colorimetric variables allowed discriminating the grapevine varieties under the contrasted hydric conditions. Under non-water stress, Carmenère was not related to any measured parameters. Merlot was positively related to most of the leaf morphometric parameters, whereas Chardonnay presented the opposite behavior. RGB color system variables allowed discriminating the grapevine varieties under water stress conditions, and Sauvignon Blanc was not related to any measured parameter. Chardonnay and Pinot Noir were positively related to green color and negatively related to most of the leaf morphometric parameters, whereas Merlot showed the opposite behavior. (4) Conclusions: Leaf morpho-colorimetric and fractal dimension parameters were affected by plant water stress and more variables should be incorporated into the new ampelographic methods in order to characterize leaf morpho-colorimetric parameters of the different grapevine varieties more clearly. [ABSTRACT FROM AUTHOR]

Breeding technologies exploiting marker-assisted selection have accelerated the selection of new cross-bred pathogen-resistant grapevine varieties. Several genotypes have been patented and admitted to cultivation; however, while their tolerance to fungal diseases has been the object of several in vitro and field studies, their productive and fruit composition traits during ripening are still poorly explored, especially in warm sites. In this study, five white pathogen-resistant varieties (PRV) listed as UD 80-100, Soreli, UD 30-080, Sauvignon Rytos, Sauvignon Kretos were tested over two consecutive seasons in a site with a seasonal heat accumulation of about 2000 growing degree days (GDDs), and their performances were compared to two Vitis vinifera L. traditional varieties, Ortrugo and Sauvignon Blanc. Berries were weekly sampled from pre-veraison until harvest to determine total soluble solids (TSS) and titratable acidity (TA) dynamics. All tested PRV exhibited an earlier onset of veraison and a faster sugar accumulation, as compared to Ortrugo and Sauvignon Blanc, especially in 2019. At harvest, Sauvignon Blanc was the cultivar showing the highest titratable acidity (8.8 g/L). Ortrugo and PRV showed very low TA (about 4.7 g/L), with the exception of Sauvignon Rytos (6.5 g/L). However, data disclose that Sauvignon Rytos higher acidity at harvest relies on higher tartrate (+1.1 to +2.2 g/L, as compared to other PRV), whereas in Sauvignon Blanc, high TA at harvest is due to either tartaric (+1 g/L, compared to PRV) and malic (+2.5 g/L, compared to PRV) acid retention. Overall, Sauvignon Rytos is the most suited PRV to be grown in a warm climate, where retaining adequate acidity at harvest is crucial to produce high-quality white wines. Nevertheless, canopy and ripening management strategies must be significantly adjusted, as compared to the standard practice employed for the parental Sauvignon Blanc. [ABSTRACT FROM AUTHOR]

Vine varieties react differently to the influence of environmental factors by decreasing or significantly intensifying the vigour of shoot growth, a biological reaction that also affects fertility and productivity. This article presents data on some agrobiological properties (vegetative growth of shoots, fertility and productivity) of some white wine varieties, created at the Research and Development Station for Viticulture and Vinification (R.D.S.V.V.) Odobeşti, Vrancea County, Romania) - Şarba, Băbească gri, Miorița and Vrancea, in the period 2020 - 2021. The data obtained were compared with the control variety Fetească regală. In Odobeşti vineyard, 2020 was much warmer than normal, characterized by a severely deficient rainfall regime resulting in atmospheric drought, followed by the gradual development of pedological drought. The vigour of shoots was negatively influenced by the climatic conditions of the vegetation period in 2020, the highest values being recorded for the varieties Şarba (159.6 cm) and Vrancea (141.5 cm), which showed significant positive differences, compared to the control variety. The fertility of the buds was lower in 2020, compared to 2021, the Vrancea variety being distinguished by higher potential fertility than the varieties Şarba, Băbească gri and Miorița, but lower potential fertility than the control variety. The absolute fertility coefficient (Cfa) had superunitary values for all varieties (1.07 - 1.50), and the relative fertility coefficient (Cfr) had subunitary values, except for the Vrancea variety (1.32). The varieties Şarba, Băbească gri and Miorița achieved an absolute productivity index (Ipa) superior to the control variety Fetească regală due to the higher average weight of the grapes, and the relative productivity index (Ipr) ranged from 199 for the Vrancea variety to 182 for the variety Şarba. [ABSTRACT FROM AUTHOR]

Nemea and Mantinia are famous wine regions in Greece known for two indigenous grape varieties, Agiorgitiko and Moschofilero, which produce high quality PDO wines. In the present study, indigenous Saccharomyces cerevisiae yeast strains were isolated and identified from spontaneous alcoholic fermentation of Agiorgitiko and Moschofilero musts in order to evaluate their oenological potential. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) recovered the presence of five distinct profiles from a total of 430 yeast isolates. The five obtained strains were evaluated at microvinifications trials and tested for basic oenological and biochemical parameters including sulphur dioxide and ethanol tolerance as well as H2S production in sterile grape must. The selected autochthonous yeast strains named, Soi2 (Agiorgitiko wine) and L2M (Moschofilero wine), were evaluated also in industrial (4000L) fermentations to assess their sensorial and oenological characteristics. The volatile compounds of the produced wines were determined by GC-FID. Our results demonstrated the feasibility of using Soi2 and L2M strains in industrial fermentations for Agiorgitiko and Moschofilero grape musts, respectively. [ABSTRACT FROM AUTHOR]

Sauvignon blanc is the most important grape cultivar within the New Zealand wine industry, and wines from the Marlborough region are renowned for their intense aromas including tropical, passionfruit, and green capsicum. Quality Sauvignon blanc wines are usually made from free run juice, although press fractions can be included. The chemical aroma composition and sensory profiles of two wine sets made from three press fractions (free run, light press and heavy press) were compared. The compounds 3-mercaptohexan-1-ol and 3-mercaptohexyl acetate were found to decrease between free run and heavily pressed wines while hexyl acetate, hexanol, and benzyl alcohol increased. The accompanying sensory analysis showed that free run wines were marked by aromas of Passionfruit/sweaty, Boxwood and Fresh green capsicum, while the heavy pressed wines were described by French vanilla/bourbon, Floral and Banana lolly attributes, consistent with the aroma chemical composition. [ABSTRACT FROM AUTHOR]

Grape canes represent a valuable source of numerous polyphenols with antioxidant properties, whose compositions vary depending on the genotype and environmental factors. Antioxidant activities of pure molecules are often reported without considering possible interactions that may occur in complex polyphenol mixture. Using UPLC-MS-based metabolomics and unsupervised classification, we explored the polyphenol variations in grape cane extracts from a collection of European varieties. Antioxidant activities were assessed using ORAC, ABTS, DPPH, FRAP, CUPRAC and chelation assays. Pairwise correlations between polyphenols and antioxidant capacities were performed to identify molecules that contributed more to the antioxidant capacities within a complex mixture of polyphenols.

The evaluation of the variety suitability regarding each appellation's specificities should be a strategy for maximizing the varieties' oenological potential while contributing to the sustainable production of quality wines, keeping their typicity and rationalizing winemaking costs. Thus, the combination of several grape physicochemical attributes, modulated by climate and vineyard characteristics, providing knowledge for each grape variety's oenological potential, is a relevant and reliable support for winemakers' decisions. To prove this hypothesis, six mature grape varieties from three harvests, each one from three vineyard parcels with different topographical conditions from Bairrada Appellation (Portugal), were studied using analysis of variance–simultaneous components analysis (ASCA). The effects of harvest year and parcel on grape berry weight, pH, titratable acidity, total sugars, total phenolics, antiradical activity, and volatile composition in free and glycosidically-linked forms were analyzed. The compositional plasticity of autochthonous varieties (white Arinto and Bical and red Baga, Castelão, and Touriga Nacional) was observed. Sauvignon Blanc grape composition was significantly modulated by harvest. This study represents an important contribution for the maintenance of varieties' biodiversity while contributing to establishing their peculiarities. Autochthonous varieties, if accurately exploited, can provide higher characteristic diversity than worldwide used varieties, an aspect to be more objectively taken into consideration by winemakers. [ABSTRACT FROM AUTHOR]

The cuticle-covered surface forms the interface between plant parts, including fruits, and their environment. The physical and chemical properties of fruit surfaces profoundly influence plant-frugivore interactions by shaping the susceptibility and suitability of the host for the attacker. Grapevine (Vitis vinifera , Vitaceae) serves as one of the various host plants of the spotted wing drosophila, Drosophila suzukii Matsumura (Diptera: Drosophilidae), which is invasive in several parts of the world and can cause major crop losses. The susceptibility of wine towards this pest species differs widely among varieties. The objective of our study was to identify physical and chemical traits of the berry surface that may explain the differences in susceptibility of five grape varieties to D. suzukii. Both preferences of adult D. suzukii and offspring performance on intact versus dewaxed (epicuticular wax layer mechanically removed) grape berries were investigated in dual-choice assays. Moreover, the morphology and chemical composition of cuticular waxes and cutin of the different varieties were analyzed. Bioassays revealed that the epicuticular wax layer of most tested grape varieties influenced the preference behavior of adult flies; even less susceptible varieties became more susceptible after removal of these waxes. In contrast, neither offspring performance nor berry skin firmness were affected by the epicuticular wax layer. The wax morphology and the composition of both epi- and intracuticular waxes differed pronouncedly, especially between more and less susceptible varieties, while cutin was dominated by ω-OH-9/10-epoxy-C18 acid and the amount was comparable among varieties within sampling time. Our results highlight the underestimated role of the epicuticular surface and cuticle integrity in grape susceptibility to D. suzukii. [ABSTRACT FROM AUTHOR]

Societal Impact Statement: Leaf shapes are critical to the identification of grapevine varieties. Historically, measures of grapevine leaves were used to enforce appellation law and leaf shape is used to this day to distinguish economically important clones that genetic analyses fail to discriminate. The ampelometry ("vine" + "process of measuring") of Pierre Galet (1921‐2019), through quantitative measurements and hand‐drawings, illuminated the beauty of grapevine diversity for the world. Here, mathematical methods rigorously quantifying hand‐drawings of grapevine leaves are presented, with implications for how we document and preserve the stunning diversity of plants we so readily see with our eyes. Summary: People have an innate ability to recognize different patterns in among plants. Quantifying these differences, that we assimilate so effortlessly with our senses, is more challenging. Grapevines are a special case in which leaf shape has been critical to identify varieties correctly. The distinct morphology of grapevine leaves lends itself to the application of mathematical approaches that quantify shape.Here, corresponding points on every grapevine leaf (landmarks) and equidistant points between landmarks (pseudo‐landmarks) were used to capture intricate, local features in grapevine leaves: the curvature of veins, lobes, and sinuses and the shapes of serrations. Using these points, averaged leaf shapes for 60 varieties of wine and table grapes were calculated that quantify shape to the same resolution as hand drawings.The overall morphological similarity of each variety to the other classified the leaves into two discrete groups: (i) deeply lobed and (ii) more entire—corresponding to the measurements of sinus depth put forward by the ampelographer Pierre Galet. Using the system of Galet, landmarks, and pseudo‐landmarks were converted into relative distance and angle measurements. Using a high number of landmarks and pseudolandmarks increased the accuracy of predicting variety correctly, compared to using a finite number of landmarks alone.The ability to identify correctly a vine variety from leaf shape will have significant implications for the wine industry, from ensuring correct scion and rootstock plantings in vineyards, to enforcing appellation law to discerning clones that are genetically indistinguishable. [ABSTRACT FROM AUTHOR]

Purpose: to assess the impact of foliar nutrition with soluble fertilizers Plantafol and Boroplus on economic and biological indicators and the efficiency of cultivation of table grape varieties in dry conditions under irrigation. The relevance of research is due to the problem of ensuring food security at the national and regional levels. Materials and methods. The subject of research was the grape varieties Codryanka (early), Rizamat (medium) and Moskovsky (late). The studies were carried out in the period 2015–2017 in Astrakhan region. Irrigation was carried out depending on the phase of development at a rate of 500–2250 cubic meters per hectare. The timing of the fertilization treatment was tied to the development phases, and the rates were in accordance with the manufacturer's recommendations. The methodological basis was M. A. Lazarevsky and B. A. Dospekhov methods. The calculation of economic efficiency was carried out on the basis of actual costs associated with technological operations, fertilization, irrigation, protective measures, collection of main and additional products. Results. The most cost-effective is the combined use of soluble foliar fertilizers Plantafol and Boroplus on grape plantations. The Rizamat variety was distinguished. Conclusions. The economic efficiency of the soluble foliar fertilizers application on grape plantations on average for 2015–2017 is the most significant in all three varieties Kodryanka, Rizamat and Moskovsky during combined application of fertilizers, especially in the Rizamat variety, when growing and receiving grape products of which the cost price decreased by 24.6 %, reaching a minimum value of 14947.4 rubles/t, profitability increased to 234.5 %, and the payback up to 3.35 rubles/rubles.

'Microbial terroir' relates to the influence of autochthonous yeasts associated with a grape cultivar on the resultant wine. Geographic region, vineyard site and topography, climate and vintage influence the biodiversity of these microbial communities. Current research focus attempts to correlate their 'microbial fingerprint' to the sensorial and chemical characteristics of varietal wines from distinct geographical wine regions. This study focuses on the minor red grape variety, Negro Saurí, which has seen a resurgence in the León Appellation of Origin in Spain as a varietal wine. An experimental vineyard at Melgarajo S.A. (42° 15′ 48.68_N 5° 9′ 56.66_W) was sampled over four consecutive vintages, with autochthonous yeasts being isolated from grapes, must and pilot-scale un-inoculated fermentations, and identified by ITS sequencing. Forty-nine isolates belonging to Metschnikowia pulcherrima, Lachancea thermotolerans, Hanseniaspora uvarum and Torulaspora delbrueckii were isolated from grapes and must, and early stages of fermentation dependent on seasonal variation. Saccharomyces cerevisiae predominated throughout fermentation, as a heterogeneous and dynamic population, with seven major biotypes identified amongst 110 isolates across four consecutive vintages. Twenty-four S. cerevisiae isolates representing five strains dominated in two or more vintages. Their persistence through fermentation warrants further validation of their oenological properties as starter cultures. [ABSTRACT FROM AUTHOR]

Microbial diversity present on grapes in wineries, and throughout fermentation has been associated with important metabolites for final wine quality. Although microbiome-metabolome associations have been well characterized and could be used as indicators of wine quality, the impact of regionality on the microbiome and metabolome is not well known. Additionally, studies between microbiome and metabolome have been conducted on single species grape such as Vitis vinifera instead of other species and interspecific hybrids. Although the Pennsylvania wine industry is relatively young compared to California, the industry has been experiencing rapid growth over the past decade and is expected to continue to grow in the future. Pennsylvania's climate of cold winters and high levels of rainfall throughout the growing season favors cultivation of interspecific hybrid grapes such as Vitis ssp. Chambourcin, one of the most commonly grown hybrid varieties in the state. Chambourcin is a prime candidate for studying the impact of regionality on microbiome-metabolome interactions as interspecific hybrid varieties could shape the future of winemaking. Here, we identify for the first time the regional distribution of microbial communities and their interactions with volatile metabolome during fermentation (0–20 days) by integrating high throughput Illumina sequencing (16S and ITS) and headspace-solid phase microextraction-gas chromatography-mass spectrometry. Analyzing 88 samples from nine wineries in the Central and East Pennsylvania regions, we observed high microbial diversity during early stages of fermentation (1–4 days) where non- Saccharomyces yeasts such as Starmerella and Aureobasidium and non- Oenococcus bacteria, Sphingomonas , likely contribute to microbial terroir to the resulting wines. Furthermore, key differentiators between two regions in Pennsylvania, as identified by LEfSe analysis, include the fungal genera Cladosporium and Kazachstania and the bacterial genera Lactococcus and Microbacterium. Moreover, 29 volatile fermentation metabolites were discriminated significantly (variable importance in projection > 1) between the two regions as shown by Partial Least Squares-Discriminant Analysis. Finally, Spearman's correlation identified regional differences of microbial-metabolite associations throughout fermentation that could be used for targeted microbiome manipulation to improve wine quality and preserve regionality. In summary, these results demonstrate the microbial signatures during fermentation and differential microorganisms and metabolites further support impact of regionality on Chambourcin wines in Pennsylvania. [ABSTRACT FROM AUTHOR]