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Plant pathogens cause diseases by suppressing plant immune response and interacting with plant cells. Investigating these interactions assists in decoding the molecular strategies the pathogen uses to overcome plant immunity. Among plant pathogens, the nematodes parasitizing various plants incur a profound impact on food production across the globe. To deal with these parasites, plants have developed a complicated defense system, including performed defenses like rigid cell walls and reinforcements acting as the first line of defense to combat any invader. Plants also have a wide diversity of constitutively released phytochemicals that are toxic to the invading microbes as their defense arsenals. Additionally, a substantial system of host responses is triggered in response to infection based on the abilities of the host plants to sense and recognize the invading pathogen. Nematodes have evolved the strategies to perceive and respond to host defense through their nervous system which help them escape, avoid, or neutralize the host plant defense systems. For developing an effective management strategy, it is crucial to understand the mechanism by which the nematode suppress the host defense. Previous reviews mainly discussed the interaction of plants with the nematodes for their immunity against nematodes. The present review will discuss the strategies employed by the plant parasitic nematodes for suppressing plant defense along with an overall insights into the basic nematode recognition mechanism and basal immunity response of the host plant. The mechanism of modulating host defense by nematodes including the role of their effectors were also discussed. The latest research progress about the release of metabolites by plants, and the mode of action of these defensive chemicals at the molecular level in combating the nematode invasion was also analyzed. [ABSTRACT FROM AUTHOR]

A study was conducted in the mountains of Magoebaskloof, Limpopo Province, where oak trees grow along the banks of the Broederstroom River. This study revealed that 22 nematode genera were associated with oak trees (Quercus robur). The most frequently occurring nematodes were Aphelenchus sp. (100%) and Plectus sp. (100%), followed by Helicotylenchus sp. (90%). This study examined the relationship between nematodes and the physicochemical properties of the soil using Pearson correlation. It uncovered that the organic matter content (OMC) had a negative correlation with the number of Panagrolaimus sp. (r = −0.770) and Hemicycliophora sp. (r = −0.674). Conversely, the sand percentage positively correlated (r = 0.695) with the number of Hemicycliophora sp. The clay content of the soil showed a positive correlation (r = 0.617) with the number of Ditylenchus. Soil pH demonstrated a significant negative correlation with Acrobeloides sp. (r = −0.877). The canonical correspondence analysis (CCA) explained 63.3% of the relationship between nematodes and soil physicochemical properties. The CCA results indicated that Ditylenchus exhibited a positive correlation with OMC, while the Panagrolaimus and Hemicycliophora species showed a negative correlation with OMC. The results indicated that none of the soil sample sites were under stress. The soil food web analysis revealed that most soil samples were nutrient-enriched with a low C/N ratio. In conclusion, this study revealed that oak trees harbor a high diversity of plant-parasitic and free-living nematodes. The results suggest that soil nematodes, particularly free-living bacterivores, such as Panagrolaimus, can indicate organic matter content in the soil. [ABSTRACT FROM AUTHOR]

In the present study, the DNA barcoding of the nematode parasite infecting Ompok bimaculatus and Nemacheilus anguilla fish species was carried out in Barvi Reservoir, Maharashtra. To ascertain the taxonomic status of these nematode parasites, an 18S gene marker was used. Accurate identification of fish parasites is essential to formulate preventive strategies and to study host–environment relations. The present study did barcoding of the nematode parasites of the fishes caught from the Barvi Reservoir using the nuclear 18S rDNA (SSU) sequence. The nuclear 18S rDNA (SSU) was amplified into two overlapping amplicons and sequenced to identify the species based on the sequence similarity with the NCBI GenBank database. The present study sequences (both fragments) showed 98% similarity with the species of Eustrongylides. The average genetic distance value between the present study sample and species of Eustrongylides was 0.003. In the phylogenetic tree also, the sequence was clustered with the species of Eustrongylides with significant bootstrap values. The present study identified the nematode parasite of the fish caught from the Barvi Reservoir, as species of Eustrongylides. The species‐level identification could not be possible due to the insufficient/lack of reference sequences in the database. It indicates the knowledge gap concerning the species‐specific molecular markers for nematode parasites of the fish. [ABSTRACT FROM AUTHOR]

The bovine lungworm, Dictyocaulus viviparus (Bloch, 1782), is highly pathogenic and disease outbreaks can be difficult to predict and manage. Rapid and accurate diagnosis is vital, but without a sensitive diagnostic test this remains challenging in clinical practice. High performance molecular detection tools are therefore required to improve the diagnosis of this parasite and promote the implementation of strategic control measures. Loop-mediated isothermal amplification (LAMP), a rapid DNA assay, offers potential for field-based detection. Here we report a novel LAMP assay (DviLAMP), that was designed to target the D. viviparus internal transcribed spacer 2 (ITS2) ribosomal DNA region. Firstly, genomic DNA was extracted from a single D. viviparus L1 larva to amplify and clone the ITS2 into the recombinant plasmid (DviITS2). The DviLAMP successfully detected the target, with results shown by gel electrophoresis and real-time analysis, in addition to point-of-care amenable end-point detection: colorimetry and lateral flow dipstick (LFD). Analytical sensitivity can detect 0.5 ng DviITS2 following 45 min of incubation at 64°C, increasing to just 1 pg following 90 min of incubation. Using the same primers, other nematodes of cattle, Ostertagia ostertagi and Cooperia oncophora, were also detectable both by gel electrophoresis and real-time. However, when FITC and biotin tagged primers were incorporated to adapt the DviLAMP to LFD end-point detection, the LFD showed specific detection of D. viviparus. Further development of DviLAMP as a point-of-care test could significantly improve the sensitivity of lungworm diagnosis in the field. [ABSTRACT FROM AUTHOR]

Background: Parascaris spp. represent a significant threat to equine health worldwide, particularly in foals. The long-term survival of parasites in the host necessitates persistent modulation of the host immune response. Intercellular communication achieved through the exchange of molecules via extracellular vesicles (EVs) released from the parasite could be a crucial factor in this regard. This study aimed to isolate and characterize EVs released by adult male and female Parascaris worms and conduct a proteomic analysis to identify sex-specific proteins and potential immunomodulatory factors. Methods: Live adult Parascaris worms were collected, and EVs were isolated from spent culture media using differential ultracentrifugation. Nanoparticle tracking analysis and transmission electron microscopy confirmed the size, concentration, and morphology of the isolated EVs. Proteins within the isolated EVs were analyzed using mass spectrometry-based proteomics (LC–MS/MS). Results: Proteomic analysis revealed a total of 113 proteins in Parascaris EVs, with several proteins showing homology to known helminth exosome proteins and exhibiting immunomodulatory functions. Sex-specific differences in EV protein composition were observed, with a distinct abundance of C-type lectins in female EVs, suggesting potential sex-specific roles or regulation. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed metabolic pathways shared between male and female Parascaris EVs, as well as differences in signal transduction, and cell growth and death pathways, indicating sex-specific variations. Conclusions: These findings imply that Parascaris EVs and their protein cargo are complex. This data potentially opens avenues for discovering innovative approaches to managing and understanding helminth infection. [ABSTRACT FROM AUTHOR]

Ageratum conyzoides L. is native to Tropical America, and it has naturalized in many other tropical, subtropical, and temperate countries in South America, Central and Southern Africa, South and East Asia, Eastern Austria, and Europe. The population of the species has increased dramatically as an invasive alien species, and it causes significant problems in agriculture and natural ecosystems. The life history traits of Ageratum conyzoides, such as its short life cycle, early reproductive maturity, prolific seed production, and high adaptive ability to various environmental conditions, may contribute to its naturalization and increasing population. Possible evidence of the molecules involved in the defense of Ageratum conyzoides against its natural enemies, such as herbivore insects and fungal pathogens, and the allelochemicals involved in its competitive ability against neighboring plant species has been accumulated in the literature. The volatiles, essential oils, extracts, residues, and/or rhizosphere soil of Ageratum conyzoides show insecticidal, fungicidal, nematocidal, and allelopathic activity. The pyrrolizidine alkaloids lycopsamine and echinatine, found in the species, are highly toxic and show insecticidal activity. Benzopyran derivatives precocenes I and II show inhibitory activity against insect juvenile hormone biosynthesis and trichothecene mycotoxin biosynthesis. A mixture of volatiles emitted from Ageratum conyzoides, such as β-caryophyllene, β-bisabolene, and β-farnesene, may work as herbivore-induced plant volatiles, which are involved in the indirect defense function against herbivore insects. Flavonoids, such as nobiletin, eupalestin, 5′-methoxynobiletin, 5,6,7,3′,4′,5′-hexamethoxyflavone, and 5,6,8,3,4′,5′-hexamethoxyflavone, show inhibitory activity against the spore germination of pathogenic fungi. The benzoic acid and cinnamic acid derivatives found in the species, such as protocatechuic acid, gallic acid, p-coumaric acid, p-hydroxybenzoic acid, and ferulic acid, may act as allelopathic agents, causing the germination and growth inhibition of competitive plant species. These molecules produced by Ageratum conyzoides may act as defense molecules against its natural enemies and as allelochemicals against neighboring plant species, and they may contribute to the naturalization of the increasing population of Ageratum conyzoides in new habitats as an invasive plant species. This article presents the first review focusing on the defense function and allelopathy of Ageratum conyzoides. [ABSTRACT FROM AUTHOR]

Patterns of lineal descent play a critical role in the development of metazoan embryos. In eutelic organisms that generate a fixed number of somatic cells, invariance in the topology of their cell lineage provides a powerful opportunity to interrogate developmental events with empirical repeatability across individuals. Studies of embryonic development using the nematode Caenorhabditis elegans have been drivers of discovery. These studies have depended heavily on high-throughput lineage tracing enabled by 4D fluorescence microscopy and robust computer vision pipelines. For a range of applications, computer-aided yet manual lineage tracing using 4D label-free microscopy remains an essential tool. Deep learning approaches to cell detection and tracking in fluorescence microscopy have advanced significantly in recent years, yet solutions for automating cell detection and tracking in 3D label-free imaging of dense tissues and embryos remain inaccessible. Here, we describe embGAN, a deep learning pipeline that addresses the challenge of automated cell detection and tracking in label-free 3D time-lapse imaging. embGAN requires no manual data annotation for training, learns robust detections that exhibits a high degree of scale invariance, and generalizes well to images acquired in multiple labs on multiple instruments. We characterize embGAN's performance using lineage tracing in the C. elegans embryo as a benchmark. embGAN achieves near–state-of-the-art performance in cell detection and tracking, enabling high-throughput studies of cell lineage without the need for fluorescent reporters or transgenics. [ABSTRACT FROM AUTHOR]

This article explores the issue of anthelmintic resistance in Europe and the environmental concerns associated with its use. It suggests sustainable worm control practices, such as diagnostics, grazing management, and selective breeding, as alternatives to reduce the reliance on anthelmintics. The article emphasizes the need for a Community of Practice across Europe to promote sustainable worm control and involve all relevant stakeholders. It provides examples of different approaches to worm control in various European countries, including Ireland, Poland, the United Kingdom, and Greece. The article also discusses the need for improved deworming practices in the ruminant livestock population in Spain, highlighting the lack of registered anthelmintics for goats and the inefficient use of deworming due to the lack of diagnostics. It suggests the use of complementary control approaches and diagnostic tools for effective and sustainable control of helminths. The authors stress the importance of implementing sustainable worm control practices across Europe and propose the establishment of local and national networks, supported by an international stakeholder network, to ensure long-term sustainability. [Extracted from the article]

Abstract Understanding the syntenic relationships among genomes is crucial to elucidate the genomic mechanisms that drive the evolution of species. The nematode Caenorhabditis is a good model for studying genomic evolution due to the well-established biology of Caenorhabditis elegans and the availability of > 50 genomes in the genus. However, effective alignment of more than ten species in Caenorhabditis has not been conducted before, and there is currently no tool to visualize the synteny of more than two species. In this study, we used Progressive Cactus, a recently developed multigenome aligner, to align the genomes of eleven Caenorhabditis species. Through the progressive alignment, we reconstructed nine ancestral genomes, analyzed the mutational types that cause genomic rearrangement during speciation, and found that insertion and duplication are the major driving forces for genome expansion. Dioecious species appear to expand their genomes more than androdioecious species. We then built an online interactive app called WormSynteny to visualize the syntenic relationship among the eleven species. Users can search the alignment dataset using C. elegans query sequences, construct synteny plots at different genomic scales, and use a set of options to control alignment output and plot presentation. We showcased the use of WormSynteny to visualize the syntenic conservation of one-to-one orthologues among species, tandem and dispersed gene duplication in C. elegans, and the evolution of exon and intron structures. Importantly, the integration of orthogroup information with synteny linkage in WormSynteny allows the easy visualization of conserved genomic blocks and disruptive rearrangement. In conclusion, WormSynteny provides immediate access to the syntenic relationships among the most widely used Caenorhabditis species and can facilitate numerous comparative genomics studies. This pilot study with eleven species also serves as a proof-of-concept to a more comprehensive larger-scale analysis using hundreds of nematode genomes, which is expected to reveal mechanisms that drive genomic evolution in the Nematoda phylum. Finally, the WormSynteny software provides a generalizable solution for visualizing the output of Progressive Cactus with interactive graphics, which would be useful for a broad community of genome researchers.

Abstract Background Parascaris spp. represent a significant threat to equine health worldwide, particularly in foals. The long-term survival of parasites in the host necessitates persistent modulation of the host immune response. Intercellular communication achieved through the exchange of molecules via extracellular vesicles (EVs) released from the parasite could be a crucial factor in this regard. This study aimed to isolate and characterize EVs released by adult male and female Parascaris worms and conduct a proteomic analysis to identify sex-specific proteins and potential immunomodulatory factors. Methods Live adult Parascaris worms were collected, and EVs were isolated from spent culture media using differential ultracentrifugation. Nanoparticle tracking analysis and transmission electron microscopy confirmed the size, concentration, and morphology of the isolated EVs. Proteins within the isolated EVs were analyzed using mass spectrometry-based proteomics (LC–MS/MS). Results Proteomic analysis revealed a total of 113 proteins in Parascaris EVs, with several proteins showing homology to known helminth exosome proteins and exhibiting immunomodulatory functions. Sex-specific differences in EV protein composition were observed, with a distinct abundance of C-type lectins in female EVs, suggesting potential sex-specific roles or regulation. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed metabolic pathways shared between male and female Parascaris EVs, as well as differences in signal transduction, and cell growth and death pathways, indicating sex-specific variations. Conclusions These findings imply that Parascaris EVs and their protein cargo are complex. This data potentially opens avenues for discovering innovative approaches to managing and understanding helminth infection. Graphical Abstract

Abstract Background There is a higher risk for nematode infections associated with outdoor-reared pigs. Next to Ascaris suum, Oesophagostomum dentatum and Trichuris suis, there is the potential of infections with other nodular worm species, Hyostrongylus rubidus, Stongyloides ransomi and Metastrongylus spp. lungworms. Next-generation sequencing methods describing the nemabiome have not yet been established for porcine nematodes. Methods FLOTAC was used for faecal egg counts of porcine gastrointestinal nematodes and lungworms in piglets, fatteners and adults individually. A nemabiome analyses based on ITS-2 gene region metabarcoding was used to differentiate strongyle species. Additionally, questionnaire data was analysed using mixed-effect regression to identify potential risk factors associated with parasite occurrences and egg shedding intensity. Results On 15 of 17 farms nematode eggs were detected. Ascaris suum, strongyles and T. suis were detected on 82%, 70% and 35% of the 17 farms, respectively. Lungworms were detected on one out of four farms with access to pasture. Strongyloides ransomi was not detected. 32% (CI 28–36%), 27% (24–31%), 5% (4–7%) and 3% (0.9–8%) of the samples where tested positive for strongyles, A. suum, T. suis and lungworms, respectively. The nemabiome analysis revealed three different strongyle species, with O. dentatum being the most common (mean 93.9%), followed by O. quadrispinulatum (5.9%) and the hookworm Globocephalus urosubulatus (0.1%). The bivariate and multivariate risk factor analyses showed among others that cleaning once a week compared to twice a week increased the odds significantly for being infected with A. suum (OR 78.60) and strongyles (2077.59). Access to pasture was associated with higher odds for A. suum (43.83) and strongyles (14.21). Compared to shallow litter systems, deep litter and free range systems resulted in significant higher odds for strongyles (85.74, 215.59, respectively) and T. suis (200.33, 623.08). Conclusions Infections with A. suum, O. dentatum, O. quadrispinulatum, T. suis, Metastrongylus spp. and G. urosubulatus are present in German outdoor-reared pigs. This is the first report of G. urosubulatus in domestic pigs in Europe. Metabarcoding based on the ITS-2 region is a suitable tool to analyse the porcine nemabiome. Furthermore, management practices have the potential of reducing the risk of parasite infections.

Mansonella ozzardi, a filarioid parasite, causes human mansonellosis in the Americas. We identified raccoons (Procyon lotor) as wildlife reservoirs of M. ozzardi in Costa Rica. Noting the sympatry of free-ranging raccoons and humans, we conclude that mansonellosis is a considerable public health risk in the region.

Zoo conditions are unique for reindeer, since domestic reindeer are not kept in captive facilities like cattle. In the zoo, reindeer are usually surrounded by many different animals that they would never encounter naturally. Thus, they might be infected with new helminths. Numerous petting zoos raise concerns about the safety of tactile interactions for human visitors. Our study is the first large-scale one. Qualitative and quantitative fecal analyses were carried out for 233 reindeer distributed over 50 Russian zoos according to the National Standard of the Russian Federation (GOST R 54627-2011) Ruminant animals—Methods of Laboratory Helminthological Diagnostics. Where possible, DNA analyses of helminths were performed targeting internal transcribed spacer region. As a result, F. hepatica, Paramphistomum sp., Moniezia sp. (including M. expansa), gastrointestinal strongylids (including Nematodirus spp.), Dictyocaulus sp., E. rangiferi, Trichuris sp., and Capillaria sp. were found in 106 (45%) zoo reindeer. All these helminths were previously reported in reindeer and pose no direct danger for humans. The intensity of invasions was mostly low. Fecal examination might be considered as an indirect method for mange diagnostics, as Chorioptes and Demodex mites were found in reindeer fecal samples. The latter may represent a novel species of mite specific for reindeer.

Parasitic otitis is an inflammatory process that can affect the external to internal cattle ear, causing discomfort in animals, impairing performance, and even leading to animal death. The infection was initially associated with nematodes of the Rhabditis genus in tropical and subtropical regions. Currently, the nematode species described as associated with bovine otitis are Metarhabditis costai, Metarhabditis freitasi, and, more recently, M. blumi. It is worth highlighting that there is still a lack of robust information regarding the morphological details, ultrastructural aspects, and molecular biology data of these species. The Metarhabditis genus is composed of seven species and two more have recently been added. The objective of this study is to update the morphological data using advanced microscopy techniques to emphasize and clarify the main morphological differences between three species of Metarhabditis currently associated with parasitic otitis. Samples of inflammatory exudate were collected from four adult female Gir cattle (Bos taurus indicus) on a farm in Itabira, Minas Gerais state, Brazil. Specimens were analyzed using light microscopy and scanning electron microscopy. Two species, M. costai and M. freitasi, were morphologically identified, consistent with previous reports. Scanning electron microscopy revealed new structural characteristics of the nematode species involved in parasitic otitis compared with M. blumi obtained from the CGC Center. Significant differences were observed in the male posterior region, bursa, and tail. Molecular analysis was conducted to differentiate these three species, and it was observed that the species first associated with otitis formed distinct clusters compared to M. blumi. However, it is important to note that further studies are needed to genetically characterize species of the Metarhabditis genus.

Abstract Plant-parasitic nematodes are a major problem for horticultural production, causing significant economic losses. Chemical nematicides are a common nematode control strategy but can negatively impact the environment and human health. In this review, we provide an overview of the use of fungi for the biological control of plant-parasitic nematodes in horticultural crops. Fungal bio-control represents an alternative, environmentally friendly strategy for managing nematodes. The use of fungi for the bio-control of nematodes has gained increasing attention due to the potential benefits of selectivity, sustainability, and long-term control. Here, we discuss the different types of fungi used for nematode management, the mechanisms of their action, the advantages and limitations of using fungi for bio-control, and the factors affecting their efficacy. Additionally, we provide several case studies of fungal bio-control against root-knot, cyst, and lesion nematodes in horticultural crops. The review concludes with a discussion of future directions and research recommendations for implementing fungal bio-control in horticultural crops.

Gebeyehu Alkadir, Dinka Ayana Department of Veterinary Pathology & Parasitology, College of Veterinary Medicine & Agriculture, Addis Ababa University, Bishoftu, EthiopiaCorrespondence: Gebeyehu Alkadir, Email gebeyehu.alkadir@aau.edu.etIntroduction: Gastrointestinal helminth parasitism is an important problem of livestock in many places of the globe. Despite the fact that the disease is one of the leading causes of economic losses, there is little information on the occurrence of the infection in cattle and sheep in Ethiopia.Methods: A cross-sectional study was conducted from June 2022 to February 2023 in urban and peri-urban areas of the Ada’a District with the objective of estimating the prevalence of gastrointestinal helminth parasites (GIHPs) in sheep and cattle and to assess the commercial anthelmintic utilization practices and associated risk factors in sheep and cattle. A total of 351 randomly selected fecal samples (192 cattle and 159 sheep) were collected from the rectum and examined using flotation and sedimentation. One hundred respondents were interviewed for questionnaire surveys on anthelmintic utilization practices.Results and Discussion: The overall prevalence of GIHPs (61.25%) was recorded during the study. Among the animals, 55.21% and 68.55% prevalence of the infection was observed in cattle and sheep, respectively. There was a statistically significant difference (P < 0.05) in gastrointestinal helminth parasite (GIHP) infection between the study animal species, breeds, body condition of the animals, and production systems. However, there were no significant differences in gastrointestinal helminth parasite (GIHP) infection (P > 0.05) according to sex and age of the animals. A higher prevalence of strongyle (55.34%), fasciola (13.48%), and moniezia eggs (10.69%) was recorded. According to a questionnaire survey, ivermectin was found to be the most used anthelmintic drug. This study addresses the challenge of anthelmintic resistance (AR) against currently available anthelmintics; highly prevalent parasitic infections are causing tremendous economic losses to the animal industry.Keywords: nematode, trematode, cestode, strongyle, fasciola, moniezia

A newspecies of nematode, Molinostrongylus longmenensis n.sp., parasite of the genus Molinostrongylus, is described based on specimens recovered from the small intestine of Scotophilus kuhlii Leach, 1822 (Chiroptera: Vespertilionidae) in Longmen County, Guangdong Province, China. To date, 135 species of bat-parasitic nematodes have been reported worldwide. Overall, 13 species belonging to seven genera in three families have been described in China. The new species is characterized by the presence of three ventral and three dorsal longitudinal cuticular ridges perpendicular to the body surface, which appear posterior to the cephalic vesicle and extend to the caudal bursa in males and the posterior end in females. The female tail has two medium-sized subventral conical processes of equal length, as well as one large dorsal conical process, and one thin spine, lateral alae that extend to the position of the vulva, with a fin-like ending. In addition, the new species was also characterized using molecular approaches, such as sequencing and analyzing the internal transcribed spacer 1 (ITS-1) of the ribosomal DNA. [ABSTRACT FROM AUTHOR]

Background: There is a higher risk for nematode infections associated with outdoor-reared pigs. Next to Ascaris suum, Oesophagostomum dentatum and Trichuris suis, there is the potential of infections with other nodular worm species, Hyostrongylus rubidus, Stongyloides ransomi and Metastrongylus spp. lungworms. Next-generation sequencing methods describing the nemabiome have not yet been established for porcine nematodes. Methods: FLOTAC was used for faecal egg counts of porcine gastrointestinal nematodes and lungworms in piglets, fatteners and adults individually. A nemabiome analyses based on ITS-2 gene region metabarcoding was used to differentiate strongyle species. Additionally, questionnaire data was analysed using mixed-effect regression to identify potential risk factors associated with parasite occurrences and egg shedding intensity. Results: On 15 of 17 farms nematode eggs were detected. Ascaris suum, strongyles and T. suis were detected on 82%, 70% and 35% of the 17 farms, respectively. Lungworms were detected on one out of four farms with access to pasture. Strongyloides ransomi was not detected. 32% (CI 28–36%), 27% (24–31%), 5% (4–7%) and 3% (0.9–8%) of the samples where tested positive for strongyles, A. suum, T. suis and lungworms, respectively. The nemabiome analysis revealed three different strongyle species, with O. dentatum being the most common (mean 93.9%), followed by O. quadrispinulatum (5.9%) and the hookworm Globocephalus urosubulatus (0.1%). The bivariate and multivariate risk factor analyses showed among others that cleaning once a week compared to twice a week increased the odds significantly for being infected with A. suum (OR 78.60) and strongyles (2077.59). Access to pasture was associated with higher odds for A. suum (43.83) and strongyles (14.21). Compared to shallow litter systems, deep litter and free range systems resulted in significant higher odds for strongyles (85.74, 215.59, respectively) and T. suis (200.33, 623.08). Conclusions: Infections with A. suum, O. dentatum, O. quadrispinulatum, T. suis, Metastrongylus spp. and G. urosubulatus are present in German outdoor-reared pigs. This is the first report of G. urosubulatus in domestic pigs in Europe. Metabarcoding based on the ITS-2 region is a suitable tool to analyse the porcine nemabiome. Furthermore, management practices have the potential of reducing the risk of parasite infections. [ABSTRACT FROM AUTHOR]

Parasitic otitis is an inflammatory process that can affect the external to internal cattle ear, causing discomfort in animals, impairing performance, and even leading to animal death. The infection was initially associated with nematodes of the Rhabditis genus in tropical and subtropical regions. Currently, the nematode species described as associated with bovine otitis are Metarhabditis costai, Metarhabditis freitasi, and, more recently, M. blumi. It is worth highlighting that there is still a lack of robust information regarding the morphological details, ultrastructural aspects, and molecular biology data of these species. The Metarhabditis genus is composed of seven species and two more have recently been added. The objective of this study is to update the morphological data using advanced microscopy techniques to emphasize and clarify the main morphological differences between three species of Metarhabditis currently associated with parasitic otitis. Samples of inflammatory exudate were collected from four adult female Gir cattle (Bos taurus indicus) on a farm in Itabira, Minas Gerais state, Brazil. Specimens were analyzed using light microscopy and scanning electron microscopy. Two species, M. costai and M. freitasi, were morphologically identified, consistent with previous reports. Scanning electron microscopy revealed new structural characteristics of the nematode species involved in parasitic otitis compared with M. blumi obtained from the CGC Center. Significant differences were observed in the male posterior region, bursa, and tail. Molecular analysis was conducted to differentiate these three species, and it was observed that the species first associated with otitis formed distinct clusters compared to M. blumi. However, it is important to note that further studies are needed to genetically characterize species of the Metarhabditis genus. [ABSTRACT FROM AUTHOR]

Zoo conditions are unique for reindeer, since domestic reindeer are not kept in captive facilities like cattle. In the zoo, reindeer are usually surrounded by many different animals that they would never encounter naturally. Thus, they might be infected with new helminths. Numerous petting zoos raise concerns about the safety of tactile interactions for human visitors. Our study is the first large-scale one. Qualitative and quantitative fecal analyses were carried out for 233 reindeer distributed over 50 Russian zoos according to the National Standard of the Russian Federation (GOST R 54627-2011) Ruminant animals—Methods of Laboratory Helminthological Diagnostics. Where possible, DNA analyses of helminths were performed targeting internal transcribed spacer region. As a result, F. hepatica, Paramphistomum sp., Moniezia sp. (including M. expansa), gastrointestinal strongylids (including Nematodirus spp.), Dictyocaulus sp., E. rangiferi, Trichuris sp., and Capillaria sp. were found in 106 (45%) zoo reindeer. All these helminths were previously reported in reindeer and pose no direct danger for humans. The intensity of invasions was mostly low. Fecal examination might be considered as an indirect method for mange diagnostics, as Chorioptes and Demodex mites were found in reindeer fecal samples. The latter may represent a novel species of mite specific for reindeer. [ABSTRACT FROM AUTHOR]

Infections of endoparasites in primates in natural habitats are highly prevalent and can cause disease, reduce health quality, and disrupt their life. This study investigated endoparasites prevalence value in the endangered Javan gibbon (Hylobates moloch) in Citalahab Forest, Gunung Halimun Salak National Park, Indonesia, from June to August 2022 by collected fecal samples (N = 10) and analyzed it using floatation methods. As a result, we found five genera of nematodes Trichuris trichiura (10% egg worm prevalence, Oesophagostomum spp. (50%), Trichostrongylus spp. (60%), Ancylostoma spp. (80%), and Strongyloides spp. (100%). The prevalence value of the worms in the larvae stage of Trichostrongylus spp. 20% and Strongyloides spp. 70%. Nematode infection status successively is Strongyloides spp., which is, frequently; Trichostrongylus spp. and Ancylostoma spp., which is, often; Oesophagostomum spp. and Trichuris trichiura which is, occasionally. Four species of nematode were found in both ages, and only Trichuris trichiura was found in one adolescent individual. The threat posed by this parasite deserves attention; further research is needed to fill the gap in our knowledge of their pathogenicity and transmission in Javan gibbon. [ABSTRACT FROM AUTHOR]

Plant pathogenic nematodes play an important role in crop production and are difficult to control. One of them is Heterodera schachtii—a cyst nematode, pathogenic to sugar beet. Nature suggests a natural way to resolve this problem by using nematode-trapping fungi, one of which is Pleurotus ostreatus. It is one of the most widespread mushrooms in the world. It is a valuable and widely cultivated edible mushroom with nematocidal properties. The mycelium of this mushroom produces toxin droplets that paralyze nematodes, after which the nematodes become infected with the hyphae filament, resulting in their death. This feature can be put to practical use as a natural nematocidal agent. In this paper, we have described studies on the variability of the nematocidal properties in the progeny of three wild strains of P. ostreatus (Po1, Po2, Po4), obtained by crossing dikaryons with monokaryons (Buller phenomenon). The toxicity of mycelium was tested against the model organism Caenorhabditis elegans and against sugar beet pest—H. schachtii. The results of the study allowed the selection of the progeny heterokaryotic mycelia with the best parameters useful for in vitro control of both nematodes. They differed in the activity against C. elegans and H. schachtii, and in the toxic knobs production. The results indicated that the progeny of the Po1 strain presented a good ability to produce hyphal knobs in the presence of C. elegans, and progenies of the Po4 strain presented good quality of growth in preferred temperatures (10–25 °C). Three strains Po1 5dix27, Po2 20dix21, and Po4 2dix1, as well as a maternal strain Po4 controlled H. schachtii by entwining cysts better than other strains. These strains were moderately effective against C. elegans. Strains Po2 15dix17, Po4 1dix18, and Po4 1dix30 may be considered good controlling isolates against both tested organisms. The results of the research also clearly suggest that the killing properties of P. ostreatus mycelia should not be assessed only by their ability to produce toxic hyphal knobs. Their nematocidal properties also depend on other mechanisms developed by mycelia, which is observed as the lethality of nematodes. The results of this research will lead to a natural way to protect plants against nematodes. The research also proved the nematocidal properties of the wild strains to reduce the H. schachtii population in the soil. [ABSTRACT FROM AUTHOR]

The marine parasitic nematode Anisakis simplex has a complex life cycle including marine mammals (mainly cetaceans) as definitive hosts, invertebrates (e.g., copepods and euphausiids) as the first paratenic hosts, and a wide range of fish species acting as second paratenic hosts. During the life cycle, the larva encounters a wide range of biotic (host immune factors and microelectric activity) and abiotic conditions (temperature and pH). We hypothesized that these factors may explain the differential behaviour of the nematode, recognized during the different life cycle stages. In this study, third-stage larvae (L3) of A. simplex were isolated from freshly caught Atlantic herring (Clupea harengus) from FAO zone 27. We exposed nematodes to different pH values (pH 2 to 9) at different temperature levels (4, 14, 21 and 37 °C), electric currents (6 mA, 12 mA, 18 mA) and different concentrations of fish immune cells. The nematode larvae exhibited significantly differential behaviour (stretched non-aggregated, spiral non-aggregated and aggregated) and activity levels when exposed to the different physicochemical conditions. We recorded negative correlations between activity and pH (maximum at pH 2) and positive correlations between activity and temperature (maximum at 37 °C). The nematode larvae were affected when exposed to electricity and fish immune cells. Electric currents at 6 mA induced minor changes, but at 12 mA and 18 mA, the majority or all nematode larvae aggregated and rolled up into spirals. Exposure to leukocytes, isolated from rainbow trout head kidney and spleen, induced a similar concentration-dependent spiralling process in larvae. We discuss these behavioural patterns of A. simplex as adaptations to conditions encountered by the worm larvae during the different stages of their complex life cycle. [ABSTRACT FROM AUTHOR]

It is not uncommon for pigeons kept for entertainment or competition to be infected with a variety of intestinal parasites, including nematodes and cestodes. Infection by these worms is a risk not only to birds but also to native wildlife and humans. The objective of this study is to assess the prevalence of helminth infections in pigeons in Zabol County, Iran, and to develop strategies for the prevention and treatment of these infections. A total of 220 pigeons were examined for the presence of parasites in their gastrointestinal tracts between 2021 and 2022. The pigeons were categorized according to gender, breed, age, medication, and feed type. The digestive tract contents were scraped and washed with warm water in order to collect intestinal helminths. A variety of techniques employed for the isolation, observation, and identification of nematodes and cestodes. Additionally,the parasites around the esophageal tissue were also examined in this study. A total of 96 positive cases of helminth infection (43.63%) were identified in the 220 samples of pigeon gastrointestinal tracts examined. In addition, 52 pigeons exhibited exclusive cestode infections, 14 had nematode infections, and 31 had co-infections. Heterakis gallinarum (H. gallinarum), Eulimdana clava (E. clava), Hadjelia truncate (H. truncate), and Ascaridia columbae (A. columbae) were among the nematodes found. Cestode species identified include Raillietina tetragona (R. tetragona), Raillietina echinobothrida (R. echinobothrida), Raillietina cesticillus (R. cesticillus), Raillietina magninumida (R. magninumida), and Cotugnia digonopora (C. digonopora). A statistical analysis of data revealed a significant correlation between helminth infection and drug consumption (p=0.001). Infection with nematodes and cestodes is also associated with pigeon age (p=0.00001). T Notably, despite the hot and dry conditions, there was a high prevalence of cestode and nematode infestations in pigeons in Zabol, Iran. It is imperative that a comprehensive control program be implemented to prevent parasite transmission to pigeons and the local avian ecosystem. [ABSTRACT FROM AUTHOR]

Adult female and male Gongylonema nematodes were found in the oesophagus of a free-living roe deer (Capreolus capreolus) in Slovenia during passive health surveillance of wildlife. The genus Gongylonema was determined by light microscopy based on the genus-specific cuticular bosses in the anterior part of the parasite. Molecular methods were used to confirm the species Gongylonema pulchrum, which has zoonotic potential. Although Gongylonema species are considered common and distributed worldwide, this is the first report of G. pulchrum in an animal on the territory of Slovenia and the first molecular report in a roe deer worldwide. The parasite is likely to be underdiagnosed, misdiagnosed or goes unnoticed as the animals show little or no clinical signs and minor pathological lesions. Slaughterhouse workers, hunters and veterinarians should be aware of this elusive parasite. Examination and evisceration of the upper digestive tract of animals should therefore be carried out more carefully. [ABSTRACT FROM AUTHOR]

Increasing evidence has suggested that nanoplastic pollution has become a global concern. More importantly, transgenerational toxicity can be induced by nanoplastics at predicted environmentally relevant doses (ERDs). Considering that amino modification could increase nanoplastic toxicity, we compared transgenerational neurotoxicity between pristine polystyrene nanoparticle (PS-NP) and amino-modified PS-NP (NH2-PS-NP) in Caenorhabditis elegans. At 0.1–10 μg/L, NH2-PS-NP caused more severe transgenerational toxicity on locomotion and neuronal development. Accompanied with a difference in transgenerational neuronal damage, compared to PS-NP (10 μg/L), NH2-PS-NP (10 μg/L) induced more severe transgenerational activation of mec-4, crt-1, itr-1, and tra-3, which are required for the induction of neurodegeneration. Moreover, NH2-PS-NP (10 μg/L) caused more severe transgenerational inhibition in expressions of mpk-1, jnk-1, dbl-1, and daf-7 than PS-NP (10 μg/L), and RNA interference (RNAi) of these genes conferred susceptibility to the toxicity of PS-NP and NH2-PS-NP on locomotion and neuronal development. NH2-PS-NP (10 μg/L) further caused more severe transgenerational activation of germline ligand genes (ins-3, ins-39, daf-28, lin-44, egl-17, efn-3, and lag-2) than PS-NP (10 μg/L), and RNAi of these ligand genes caused resistance to the toxicity of PS-NP and NH2-PS-NP on locomotion and neuronal development. Our results highlighted more severe exposure risk of amino-modified nanoplastics at ERDs in causing transgenerational neurotoxicity in organisms. [ABSTRACT FROM AUTHOR]

Plant-parasitic nematodes are a major problem for horticultural production, causing significant economic losses. Chemical nematicides are a common nematode control strategy but can negatively impact the environment and human health. In this review, we provide an overview of the use of fungi for the biological control of plant-parasitic nematodes in horticultural crops. Fungal bio-control represents an alternative, environmentally friendly strategy for managing nematodes. The use of fungi for the bio-control of nematodes has gained increasing attention due to the potential benefits of selectivity, sustainability, and long-term control. Here, we discuss the different types of fungi used for nematode management, the mechanisms of their action, the advantages and limitations of using fungi for bio-control, and the factors affecting their efficacy. Additionally, we provide several case studies of fungal bio-control against root-knot, cyst, and lesion nematodes in horticultural crops. The review concludes with a discussion of future directions and research recommendations for implementing fungal bio-control in horticultural crops. [ABSTRACT FROM AUTHOR]

We report a case of infection with the zoonotic nematode Gongylonema sp. in a 24-year-old woman from Varna, Bulgaria. Two days before seeking medical attention, the patient had noticed a filamentous mass in her buccal mucosa that migrated when touched with the tongue. She manually removed a thread-like worm from the mucosa near her lower lip. Albendazole was prescribed for three days. The patient had no history of travelling abroad or contact with animals. To our knowledge, this is the sixth case that has been reported in the country. [ABSTRACT FROM AUTHOR]

The Old-World bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae), is a significant threat to crops worldwide and has become resistant to traditional synthetic insecticides. The present study investigated the pathogenicity of the entomopathogenic fungi (EPF) Metarhizium robertsii (Hypocreales: Clavicipitaceae) strain WG-04 or Beauveria bassiana (Hypocreales: Cordycipitaceae) strain WG-10, and an entomopathogenic nematode (EPN) species Heterorhabditis bacteriophora against the second and fourth instar larvae of H. armigera. Both fungal species and H. bacteriophora were evaluated, singly or in combination. After 24 and 48 h post-application of the fungal spores (106 spores/mL), H. bacteriophora was introduced at a rate of 50 infective juveniles/mL, and mortality was observed at 3-, 5-, and 7-days post-treatment. Adult emergence, egg hatching, and percentage pupation were recorded. The integration of both types of biocontrol agents exhibited additive and synergistic interactions in larval stages, and enhanced mortality was recorded when EPF was used in combination with the nematodes. In the individual application of all three biocontrol agents alone, the order of efficacy was H. bacteriophora > B. bassiana > M. robertsii; however, in joint treatments, the increase in mortality and decrease in percentage egg hatching, pupation, and adult emergence was determined to be directly linked to the exposure period of the H. bacteriophora. The results of this study suggest that combining H. bacteriophora with EPF could provide a solid foundation for an economically viable method for managing H. armigera larvae in chickpea fields. [ABSTRACT FROM AUTHOR]

Invasive plants are capable of homogenizing both aboveground and belowground biota and, along with climate change, are recognized as one of the biggest threats to global biodiversity. Soil nematode communities reflect the surroundings they inhabit and are therefore frequently employed as biological indicators of soil condition. In this study, soil properties and nematode communities in Carpathian beech forest floor covered by dense vegetation of invasive Impatiens parviflora (small balsam) were investigated over two vegetation seasons. We assumed that the spread of invasive I. parviflora could influence soil fauna through litter accumulation when established and could also change several soil properties, consequently altering soil nematode communities. A total of 52 nematode species were found in the soil samples. The mean number of species varied from 18 to 31, but did not significantly differ between invaded and uninvaded plots across all sampling dates. However, redundancy analysis indicated that the nematode community in plots with small balsam differed significantly from that in uninvaded plots, reflecting different proportions of genera in the two communities. Invasion by small balsam significantly enhanced the relative abundance of bacterivores, whereas it decreased the abundance of plant parasites and root-fungal feeders, mainly in the spring and summer season. Ordination of nematode species along the structure index and enrichment index trajectories revealed a maturing food web, low to moderately disturbed in the I. parviflora invaded soils as well as in uninvaded forest plots. Decomposition channels of soil food webs in both plots were balanced and fungal–bacterial mediated, although low values of the channel index suggested prevailing bacterial decomposition. Our study reveals that the expansion of I. parviflora moderately influenced the composition of nematode communities and the soil food web, increased soil nitrogen, carbon and C/N ratio, but did not modify soil acidity. [ABSTRACT FROM AUTHOR]

Purpose: To report the application of an infrared fundus imaging and navigated laser system to photocoagulate a nematode in diffuse unilateral subacute neuroretinitis (DUSN). Observations: A 14-year-old boy with DUSN was treated with systemic albendazole and corticosteroids. Laser photocoagulation of the visible nematode was performed using a navigated laser in live infrared fundus view (Navilas 577s, OD-OS GmbH, Berlin, Germany). While the localization of the nematode was difficult in regular fundoscopy due to the light-shy helminth, it could be well localized and targeted with the infrared live video mode and navigated laser system. No inflammatory flare-up was observed after the nematode was killed. Conclusions and Importance: Laser photocoagulation and systemic antihelminthic therapy are an established treatment for DUSN. Infrared imaging and navigated laser systems seem useful in targeting and killing mobile nematodes.

This study focused on the nematicidal potential of sea urchin-like zinc oxide nanometrics (SUZN) against the root-knot nematode Meloidogyne incognita. XRD, SEM, UV-vis spectroscopy, TEM, and EDX have been utilized to characterize the SUZN. Various SUZN concentrations (150–750 ppm) were examined for their implications for M. incognita second-stage juveniles (J2s) and egg masses, including mortality as well as hatching inhibition. The largest J2s hatching inhibition (83.07%) was seen at 750 ppm SUZN after 48 hours of incubation, whereas the lowest hatching occurred at 150 ppm when compared to the control. SUZN illustrated increased nematicidal efficiency with increasing concentration and incubation duration. LC50 values for J2s were calculated at various time points. In root-dip trials, all SUZN doses substantially reduced J2s penetration into eggplant roots relative to the untreated control. The study emphasizes SUZN’s strong nematicidal activity against M. incognita, suggesting its promise as a long-term alternative to chemical nematicides for root-knot nematode management while reducing the environmental concerns associated with traditional pesticides.

I summarize my recent theorizing on orientation and navigation across life. Organisms use navigational servomechanisms working with oscillators to get to goals. Navigational servomechanisms track errors from the best direction of travel and initiate action to correct the error. They work with endogenously generated action patterns, oscillations produced by oscillators, to adjust the course of travel. The theme applies to all scales of life from micrometers to thousands of kilometers. Servomechanisms and oscillators also characterize some other domains of cognition.

Plant pathogens cause diseases by suppressing plant immune response and interacting with plant cells. Investigating these interactions assists in decoding the molecular strategies the pathogen uses to overcome plant immunity. Among plant pathogens, the nematodes parasitizing various plants incur a profound impact on food production across the globe. To deal with these parasites, plants have developed a complicated defense system, including performed defenses like rigid cell walls and reinforcements acting as the first line of defense to combat any invader. Plants also have a wide diversity of constitutively released phytochemicals that are toxic to the invading microbes as their defense arsenals. Additionally, a substantial system of host responses is triggered in response to infection based on the abilities of the host plants to sense and recognize the invading pathogen. Nematodes have evolved the strategies to perceive and respond to host defense through their nervous system which help them escape, avoid, or neutralize the host plant defense systems. For developing an effective management strategy, it is crucial to understand the mechanism by which the nematode suppress the host defense. Previous reviews mainly discussed the interaction of plants with the nematodes for their immunity against nematodes. The present review will discuss the strategies employed by the plant parasitic nematodes for suppressing plant defense along with an overall insights into the basic nematode recognition mechanism and basal immunity response of the host plant. The mechanism of modulating host defense by nematodes including the role of their effectors were also discussed. The latest research progress about the release of metabolites by plants, and the mode of action of these defensive chemicals at the molecular level in combating the nematode invasion was also analyzed.

I summarize my recent theorizing on orientation and navigation across life. Organisms use navigational servomechanisms working with oscillators to get to goals. Navigational servomechanisms track errors from the best direction of travel and initiate action to correct the error. They work with endogenously generated action patterns, oscillations produced by oscillators, to adjust the course of travel. The theme applies to all scales of life from micrometers to thousands of kilometers. Servomechanisms and oscillators also characterize some other domains of cognition. [ABSTRACT FROM AUTHOR]

The editorial in "Frontiers in Microbiology" discusses the significant role of Wolbachia, an endosymbiotic bacteria found in insects, arthropods, and nematodes, in various biological processes such as reproductive phenotypes and host fitness. The research on Wolbachia has led to advancements in understanding host-microbe interactions, as well as applications in controlling vector-borne diseases and pests. The editorial highlights studies on Wolbachia-induced reproductive manipulations, population dynamics, and genomic/transcriptomic analyses, providing valuable insights into this field of research. The document also mentions the 11th Wolbachia Conference held in 2023 and previews the upcoming 12th Wolbachia Conference in 2025. [Extracted from the article]

Bacterivore nematodes are the most abundant animals in the biosphere, largely contributing to global biogeochemistry. Thus, the effects of environmental microbes on the nematodes' life-history traits are likely to contribute to the general health of the biosphere. Caenorhabditis elegans is an excellent model to study the behavioral and physiological outputs of microbial diets. However, the effects of complex natural bacterial assemblies have only recently been reported, as most studies have been carried out with monoxenic cultures of laboratory-reared bacteria. Here, we quantified the physiological, phenotypic, and behavioral traits of C. elegans feeding on two bacteria that were coisolated with wild nematodes from a soil sample. These bacteria were identified as a putative novel species of Stenotrophomonas named Stenotrophomonas sp. strain Iso1 and a strain of Bacillus pumilus designated Iso2. The distinctive behaviors and developmental patterns observed in animals fed with individual isolates changed when bacteria were mixed. We studied in more depth the degeneration rate of the touch circuit of C. elegans and show that B. pumilus alone is protective, while the mix with Stenotrophomonas sp. is degenerative. The analysis of the metabolite contents of each isolate and their combination identified NAD+ as being potentially neuroprotective. In vivo supplementation shows that NAD+ restores neuroprotection to the mixes and also to individual nonprotective bacteria. Our results highlight the distinctive physiological effects of bacteria resembling native diets in a multicomponent scenario rather than using single isolates on nematodes. IMPORTANCE Do behavioral choices depend on animals' microbiota? To answer this question, we studied how different bacterial assemblies impact the life-history traits of the bacterivore nematode C. elegans using isolated bacteria found in association with wild nematodes in Chilean soil. We identified the first isolate, Iso1, as a novel species of Stenotrophomonas and isolate Iso2 as Bacillus pumilus. We find that worm traits such as food choice, pharyngeal pumping, and neuroprotection, among others, are dependent on the biota composition. For example, the neurodegeneration of the touch circuit needed to sense and escape from predators in the wild decreases when nematodes are fed on B. pumilus, while its coculture with Stenotrophomonas sp. eliminates neuroprotection. Using metabolomics analysis, we identify metabolites such as NAD+, present in B. pumilus yet lost in the mix, as being neuroprotective and validated their protective effects using in vivo experiments.

Abstract Background Nematodes are the most abundant and diverse metazoans on Earth, and are known to significantly affect ecosystem functioning. A better understanding of their biology and ecology, including potential adaptations to diverse habitats and lifestyles, is key to understanding their response to global change scenarios. Mitochondrial genomes offer high species level characterization, low cost of sequencing, and an ease of data handling that can provide insights into nematode evolutionary pressures. Results Generally, nematode mitochondrial genomes exhibited similar structural characteristics (e.g., gene size and GC content), but displayed remarkable variability around these general patterns. Compositional strand biases showed strong codon position specific G skews and relationships with nematode life traits (especially parasitic feeding habits) equal to or greater than with predicted phylogeny. On average, nematode mitochondrial genomes showed low non-synonymous substitution rates, but also high clade specific deviations from these means. Despite the presence of significant mutational saturation, non-synonymous (dN) and synonymous (dS) substitution rates could still be significantly explained by feeding habit and/or habitat. Low ratios of dN:dS rates, particularly associated with the parasitic lifestyles, suggested the presence of strong purifying selection. Conclusions Nematode mitochondrial genomes demonstrated a capacity to accumulate diversity in composition, structure, and content while still maintaining functional genes. Moreover, they demonstrated a capacity for rapid evolutionary change pointing to a potential interaction between multi-level selection pressures and rapid evolution. In conclusion, this study helps establish a background for our understanding of the potential evolutionary pressures shaping nematode mitochondrial genomes, while outlining likely routes of future inquiry.

The purpose of the research is to study pathology findings in heterakidosis in broilers.Materials and methods. The study used pathological material (cecum, liver) from 8 laying hens aged one year. Organ samples were delivered to the Pathology Sector in 10% buffered formalin. They were fixed for 36 hours and examined histologically using paraffin embedding. Semi-automatic Thermo Scientific equipment was used to process tissue samples. Histologic specimens were stained with hematoxylin and eosin. The specimen histoarchitecture was assessed with an Axio A1.0 microscope; photographs were taken using the AxioVision software.Results and discussion. Visual assessment of the pathological material revealed thickening of the distal cecum while the liver samples had no signs of pathology. It was found that Heterakis spp. was about 1 cm long; helminth eggs were oval-shaped and had a strong, uniform shell; their size was 49 × 26 micrometers in the mean. When dwelling in the cecum, Heterakis spp. causes atony of the distal cecum wall, and development of inflammatory and necrotic processes in the intestinal mucosa, which promotes active growth of bacterial flora, accumulation of bacterial byproducts and intoxication. In places where the helminth is localized, eggs accumulate and a risk of Histomonas spp. infection increases, namely, amoebic (in the intestinal lumen) and spherical (in the intestinal mucosa) forms of the protozoan were identified. It was not possible to differentiate Histomonas spp. in assessing the histological specimens of the liver from the infected birds. In diagnostics, we should consider the great similarity of morphological characteristics of Heterakis spp. and Ascaridia spp. eggs.

Equine strongylid parasites are ubiquitous around the world and are main targets of parasite control programmes. In recent years, automated fecal egg counting systems based on image analysis have become available allowing for collection and analysis of large-scale egg count data. This study aimed to evaluate equine strongylid fecal egg count (FEC) data generated with an automated system over three years in the US with specific attention to seasonal and regional trends in egg count magnitude and sampling activity. Five US regions were defined; North East, South East, North Central, South Central and West. The data set included state, region and zip code for each FEC. The number of FECs falling in each of the following categories were recorded: (1) 0 eggs per gram (EPG), (2) 1 ⩽ 200 EPG, (3) 201 ⩽ 500 EPG and (4) >500 EPG. The data included 58 329 FECs. A fixed effects model was constructed fitting the number of samples analysed per month, year and region, and a mixed effects model was constructed to fit the number of FECs falling in each of the 4 egg count categories defined above. The overall proportion of horses responsible for 80% of the total FEC output was 18.1%, and this was consistent across years, months and all regions except West, where the proportion was closer to 12%. Statistical analyses showed significant seasonal trends and regional differences of sampling frequency and FEC category. The data demonstrated that veterinarians tended to follow a biphasic pattern when monitoring strongylid FECs in horses, regardless of location.

Abstract Mitogen-activated protein kinase (MAPK) activation is one of the significant immune events that respond to pathogens in plants. A MAPK cascade often contains a MAPK kinase kinase (MAPKKK), a MAPK kinase (MAPKK/MKK), and a MAPK. The well-characterized MAPK cascade, to date, is the MAPKKK3/4/5-MKK4/5-MPK3/6 module. Soybean cyst nematodes (SCN) is one of the most devastating soybean pathogens. However, the early immune components contributing to soybean resistance to SCN and the role of the MAPK cascade in the soybean–SCN interaction remain unclear. A recent study published in Plant Cell discovered that GmMPK3/6 phosphorylates a receptor-like cytoplasmic kinase (RLCK), CDG1-LIKE1 (GmCDL1), and maintains the stability of GmCDL1 in soybean. Remarkably, GmCDL1 enhances GmMPK3/6 activation and resistance to SCN by phosphorylating GmMAPKKK5 and activating the GmMAPKKK5-GmMKK4-GmMPK3/6 cascade. In addition, two L-type lectin receptor kinases (LecRKs), GmLecRK02g and GmLecRK08g, are involved in the GmCDL1 function after the perception of SCN. taken together, this study not only discovers a complete early immune pathway that responds to SCN infection in soybean, but also reveals a molecular mechanism by which plants maintain the activation of the MAPK cascade and resistance.

Dirofilariasis is a helminthiasis that occurs in carnivorous animals (dogs, cats, foxes, wolves, etc.) and less often in humans, although according to some authors it is believed that the number of infected animals approximately corresponds to the number of infected people. Dirofilariasis of the maxillofacial area is not a widespread condition, its cases are described in short series, therefore, the aim of this work is to share our own experience of managing patients with this rare condition.The work presents our own experience in the management of 14 dirofilariasis patients with lesions of the maxillofacial area, of them there are 3 men and 11 women aged 29 to 54. Most patients sought help in the late autumn, winter and spring periods and only 2 people in the summer period. All patients were examined by a dentist, and an ultrasound examination of the area with a formation was performed. According to the clinical manifestations, the disease began acutely in nine patients and had a picture of inflamed atheroma (2 – in the zygomatic area, 1 – in the parotid, 2 – in the infraorbital area, 2 – in the temporal area, and 2 – in the area of the nasal bridge). All patients were operated on and tumor-like formations in dense membranes were removed, inside which one live nematode 11-18 cm long was found. In 3 observations, fragments of dead, partially lysed nematodes were removed from the oral cavity, the capsules of which imitated a migrating granuloma. According to the location – 5 capsules with dirofilaria were located in the subcutaneous fatty tissue, 3 – in the submucosal layer of the gums fused to the periosteum of the upper jaw, 1 – in the submucous layer of the lower jaw, 2 – in the fibers of the temporalis muscle and 1 – in the fibers of the buccal muscle. In each case, the localization of the nematode was close to the places of accumulation of adipose tissue or large blood vessels. In the postoperative period complex therapy was prescribed, including symptomatic anti-inflammatory and desensitizing agents, and consultation with infectionist was recommended. Thus, according to our observations, dirofilariasis most often affects areas of the face in which there are large vessels and accumulation of fatty tissue. In connection with the prevalence of dirofilariosis in humans and the predominant localization of parasitic infection in the face area, dentists, ophthalmologists and otolaryngologists need to be familiar with this pathology and show definite vigilance. Treatment dirofilariosis patients should be carried out comprehensively, together with parasitologists, with surgical excision of the lesion and appropriate drug therapy.

Background: Nematodes are the most abundant and diverse metazoans on Earth, and are known to significantly affect ecosystem functioning. A better understanding of their biology and ecology, including potential adaptations to diverse habitats and lifestyles, is key to understanding their response to global change scenarios. Mitochondrial genomes offer high species level characterization, low cost of sequencing, and an ease of data handling that can provide insights into nematode evolutionary pressures. Results: Generally, nematode mitochondrial genomes exhibited similar structural characteristics (e.g., gene size and GC content), but displayed remarkable variability around these general patterns. Compositional strand biases showed strong codon position specific G skews and relationships with nematode life traits (especially parasitic feeding habits) equal to or greater than with predicted phylogeny. On average, nematode mitochondrial genomes showed low non-synonymous substitution rates, but also high clade specific deviations from these means. Despite the presence of significant mutational saturation, non-synonymous (dN) and synonymous (dS) substitution rates could still be significantly explained by feeding habit and/or habitat. Low ratios of dN:dS rates, particularly associated with the parasitic lifestyles, suggested the presence of strong purifying selection. Conclusions: Nematode mitochondrial genomes demonstrated a capacity to accumulate diversity in composition, structure, and content while still maintaining functional genes. Moreover, they demonstrated a capacity for rapid evolutionary change pointing to a potential interaction between multi-level selection pressures and rapid evolution. In conclusion, this study helps establish a background for our understanding of the potential evolutionary pressures shaping nematode mitochondrial genomes, while outlining likely routes of future inquiry. [ABSTRACT FROM AUTHOR]

Simple Summary: There are growing concerns about wildlife and livestock interactions and the impacts of those interactions on the sustainability of livestock. One of those concerns is the spillover of wildlife pathogens, including helminths, into livestock. This concern will likely become realized as the demand for free-range animal products increases. One such helminth with spillover potential is the eyeworm Oxyspirura petrowi. This eyeworm is common in many wild birds, and particularly common in Northern bobwhite quail. Related helminths are already commonly found in poultry raised in free-range conditions in developing nations. The purpose of this research was to investigate the lethality of fenbendazole, a widely available drug for treating parasites, to these eyeworms. The lethality estimates were similar to estimates of lethality to other roundworm parasites. However, studies that have investigated concentrations in host blood following administration of the drug indicate that it does not stay in the system long enough to achieve elimination of the parasite after a single dose. This indicates that in order to effectively treat eyeworm, fenbendazole must be delivered in a repeated or continuous manner. Oxyspirura petrowi is a heteroxenous nematode that infects the harderian gland and other ocular tissues in birds. High-intensity infections often cause damage to the infected tissues. Due to the nature of the infection sites, treatment of O. petrowi in these hosts can be difficult. Fenbendazole (FBZ) is a common anthelmintic used to treat birds for helminth infections; however, little information exists as to the efficacy of the drug on O. petrowi infections. The present study aims to estimate lethal concentrations of FBZ to O. petrowi. Adult O. petrowi were maintained in vitro and exposed to doses of 5, 50, 100, and 200 µM concentrations of FBZ and included both negative and vehicle controls. Exposure lasted 7.5 days and lethality was determined for each treatment. Negative and vehicle controls did not differ, and both had 75% survival at the end of the treatment period. The percentage survivorship in ascending order of concentration, corrected for the controls, was 66.67%, 44.44%, 33.33%, and 0%. LC10, LC50, and LC90 estimates were 7.5 ± 0.26, 49.1 ± 1.69, and 163.2 ± 5.63 µM, respectively. In the context of known pharmacokinetics of FBZ in birds, a single oral dose of FBZ can achieve exposure levels that are lethal to O. petrowi, but the drug does not stay in the system long enough. Thus, treatment of O. petrowi infections will require multiple oral doses over several days. [ABSTRACT FROM AUTHOR]

The article presents the first overview of animal invasions in Armenia with a national comprehensive combined list of documented invasive alien animal species. This list includes 40 species from various taxa of invertebrates and vertebrates, including nematodes, annelids, crustaceans, insects, mollusks, fish and mammals. For each species, we present data on the range of invasion in the territory of Armenia, indicating the corresponding naturalization time and introduction pathway. The number of invasions has been growing exponentially since 1940. Fifteen out of the overall 40 species have been registered in the last five years. Most of the invertebrate species were likely introduced unintentionally, while vertebrate species were mainly intentionally introduced through release into the nature or escape from aquaculture or farming. Importantly, the inventory of invasive alien animal species in Armenia, which could potentially cause negative effects on the economy, nature, or human health, will aid in risk assessment and development of a country- and region-wide management policy for biological invasions. [ABSTRACT FROM AUTHOR]

Transgenerational nanoplastic toxicity could be detected in Caenorhabditis elegans after exposure at the parental generation (P0-G); however, the underlying mechanisms remain largely unclear. We aimed to examine the role of germline nuclear hormone receptors (NHRs) in controlling the transgenerational toxicity of polystyrene nanoparticles (PS-NPs) based on gene expression screening and functional analysis. Among germline NHR genes, daf-12, nhr-14, and nhr-47 expressions were increased and nhr-12 expression was decreased by PS-NPs (1 and 10 μg/L). Transgenerational alterations in expressions of these four NHR genes were also induced by PS-NPs (1 and 10 μg/L). RNAi of daf-12, nhr-14, and nhr-47 caused resistance, whereas RNAi of nhr-12 conferred susceptibility to transgenerational PS-NP toxicity. After PS-NP exposure, expressions of ins-3, daf-28, and ins-39 encoding insulin ligands, efn-3 encoding Ephrin ligand, and lin-44 encoding Wnt ligand, as well as expressions of their receptor genes (daf-2, vab-1, and/or mig-1), were dysregulated by the RNAi of daf-12, nhr-14, nhr-47, and nhr-12. Therefore, alteration in certain germline NHRs could mediate the induction of transgenerational nanoplastic toxicity by affecting secreted ligands and their receptors in the offspring of exposed organisms. [ABSTRACT FROM AUTHOR]

Species of Pterygodermatites are spirurid nematodes that have expanded their geographic distribution worldwide. They infect a variety of mammalian definitive hosts with few reports of potential paratenic infections in amphibian and reptile hosts. In this study, we report Pterygodermatites sp. larvae identified in free-ranging, invasive Cuban treefrogs (Osteopilus septentrionalis), from central Florida, United States. Encysted larvae were recovered from the skeletal muscle and/or the coelomic cavity of three frogs; molecular characterization of the small subunit (18S) ribosomal RNA and cytochrome oxidase I genes of the parasites matched reported sequences of Pterygodermatites (Mesopectines) whartoni (Tubangui, 1931). This is a parasite native to Southeastern Asia and to the best of the authors' knowledge, it is the first report of the species in the New World. The recovery of invasive Pterygodermatites from invasive Cuban treefrogs in North America highlights the growing concern regarding the potential impact non-native parasites and invasive species may have on native wildlife populations. [ABSTRACT FROM AUTHOR]