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Long-term changes of soil nematode diversity and distribution patterns in replant peach orchard (RPO) and continuous-planting peach orchard (CPPO) were assessed to evaluate different effects on nematode community and function by RPO and CPPO, in relation to peach replant problem from the point of soil fauna. Observations were conducted on a silt loam soil, and soil sampling was performed four times through the growing season of peach trees in the period from 2006 to 2008 in Pinggu, Beijing. The result showed that RPO differed from CPPO by its higher abundance of plant feeding nematodes but lower abundance of bacterivore nematodes, as well as significantly higher values of plant parasite index but lower nematode biodiversity. Obviously, the absolute abundance of Paratylenchus in RPO was higher than that in CPPO, which could be a key factor for the peach replant problems from the part of soil nematode. Nematode function indices and weighted nematode fauna profile analysis were no significantly different in the two peach ecosystems, but they represented an indication of high disturbance, N-enriched, bacterial decomposition pathway.