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This essay proposes Animal Drag as an activist performance in which a human critically and consciously performs animality to de-centre the human. Dress and adornment are key to its dissidence. Animal Drag's potential is demonstrated through an analysis of Terry Notary's performance of a nonhuman primate within Ruben Östlund's 2017 film The Square. Drawing on posthuman studies, scholarship on subordinated groups and from the field of animal studies, the reading of this performance shows how Animal Drag inherently queers humanist and essentialist notions of classification, particularly the human / animal divide. Paying homage to drag, the essay demonstrates how the material and social semiotics of performance can render transparent the constructions of race, coloniality, ability, and gender, as much as species -- in other words, "doing human". Through theories of dress and prosthesis, highlighting how techne facilitates in-between and "becoming" states, and affect theory, this article argues that humans and nonhumans might share vulnerabilities via Animal Drag. Culture is understood through language and dress, which have been mobilised to construct Otherness. As such, this essay posits that Animal Drag should take place within capitalist Culture industries, as both weapons and targets, to undress powers present and performed. Animal Drag is offered as both practice and theory, content and form. [ABSTRACT FROM AUTHOR]

The predominant approach of protecting or restoring floral and faunal life after harming, displacing, or destroying them in service of human interests does not hold much promise for nature on Earth in the age of the Anthropocene. Such approaches fail to address the ethical and political-economic cores of what tend to be presented as techno-scientific or ecological problems. If the planet is to remain home to life beyond the human, mainstream human societies need to rethink their place, role, and entitlements on Earth, and relearn to cohabit with human and nonhuman others, even in the face of risk and uncertainty. [ABSTRACT FROM AUTHOR]

Artificial intelligence technologies were developed to assist authors in bettering the organization and caliber of their published papers, which are both growing in quantity and sophistication. Even though the usage of artificial intelligence tools in particular ChatGPT's natural language processing systems has been shown to be beneficial in research, there are still concerns about accuracy, responsibility, and transparency when it comes to the norms regarding authorship credit and contributions. Genomic algorithms quickly examine large amounts of genetic data to identify potential disease-causing mutations. By analyzing millions of medications for potential therapeutic benefits, they can quickly and relatively economically find novel approaches to treatment. Researchers from several fields can collaborate on difficult tasks with the assistance of nonhuman writers, promoting interdisciplinary research. Sadly, there are a number of significant disadvantages associated with employing nonhuman authors, including the potential for algorithmic prejudice. Biased data may be reinforced by the algorithm since machine learning algorithms can only be as objective as the data they are trained on. It is overdue that scholars bring forth basic moral concerns in the fight against algorithmic prejudice. Overall, even if the use of nonhuman authors has the potential to significantly improve scientific research, it is crucial for scientists to be aware of these drawbacks and take precautions to avoid bias and limits. To provide accurate and objective results, algorithms must be carefully designed and implemented, and researchers need to be mindful of the larger ethical ramifications of their usage. [ABSTRACT FROM AUTHOR]

The present paper seeks to lay bare how the grim realities of settler colonialism and petro-capitalism have a tolling effect on the indigenous ways of life. The present era, termed the 'Anthropocene' or 'Capitalocene,' as it is the capitalist concern of some of the privileged anthropos that inflict socio-economic and ecological injustices on earth, heralds the birth of cautionary literature that deconstructs anthropocentric fixation to purge the earth off the catastrophic impacts of rapacious human activities. In order to examine how capitalism and its resultant climate emergency have spurred environmental activists and authors to reflect upon this theme, the paper undertakes a close textual analysis of The Breathing Hole (2020) by Colleen Murphy (b. 1954), a contemporary Quebecois playwright. By employing indigenous ecocriticism and non-human turn in literary studies, the study examines how the playwright jettisons the standardised category of the Anthropos by prioritising the non-human character, Angu'ruaq, an anthropomorphised polar bear. Attempts have been made to highlight how the capitalist motive of the neo-colonial agents leaves a breach in the fabric of the succouring connection between the indigenous Inuit existence and the non-human beings in the Nunavut territory of the Canadian Arctic. The findings of the paper demonstrate how the playwright approaches contemporary eco-crisis using indigenous ecodrama. [ABSTRACT FROM AUTHOR]

The research's main objective is to explore and encourage modes of architectural practice that can foster multispecies co-living to reduce biodiversity loss and increase the quality of life for both human and nonhuman inhabitants of architecture. This is achieved through conceptual discussions, comprehensive architectural case studies and work-based design explorations that support cross-species co-living in the context of Eastern Norway (Østlandet)—a geographical region of south-eastern Norway consisting of the counties Vestfold, Telemark, Viken, Oslo and Innlandet. A pluralistic method builds on analytical, critical and work-based explorative studies consisting of two parts: (a) historical and contemporary case studies in Norway that support modes of cross-species co-living and (b) design explorations by the second author investigating the operational potential of kindness in architecture. The notion of kindness in this research is built upon an understanding of the amalgam of concepts: solidarity, kinship and being kind, explained in the article's introduction. The potential for designing with and for nonhumans to reinvigorate modes of co-living and support existing habitats is investigated, focusing on the ways three bird species relate to a specific building in Eastern Norway due to their habitat needs in the region: Cyanistes caeruleus, Eurasian blue tit (blåmeis in Norwegian); Passer montanus, Eurasian tree sparrow (pilfink in Norwegian); and Delichon urbicum, northern or common house martin (taksvale in Norwegian). The research contributes to ongoing discussions within architectural discourse regarding multispecies inhabitation and architecture's role in the current biodiversity crisis and provides insight into both historical and contemporary/ongoing design solutions for multispecies co-living. [ABSTRACT FROM AUTHOR]

Loneliness and isolation were two factors introduced as "effective measures" during the COVID-19 crisis. The lockdown exacerbated loneliness among those already suffering from acute illnesses. In this context, a rereading of the film Lars and the Real Girl by Craig Gillespie is particularly relevant as it offers novel perspectives on loneliness. The interplay between Lars's desire to be in a compassionate relationship and the fear of meeting and socializing is comparable to what was witnessed across the coronavirus-afflicted world. This paper explores the potential for understanding delusion caused by traumatic experiences as a form of communication rather than a mental disorder. The film explains how a silicone sex doll functions as a medium between the lonesome Lars and society in resolving the trauma. The paper focuses on the infantile nature of humans and uses infantilism in a conducive manner to understand anthropomorphism for bridging the gap between a lonely/delusional person and society while drawing examples from the film. The introduction of a nonhuman actor—an anatomically correct doll—becomes an opportunity for a traumatized person such as Lars to know himself well and gradually open up to socializing. As he moves from external to threshold en-rolling, followed by internal en-rolling, it indicates his opening up to communication as he moves from language to lalangue and creates his world with the doll. This film presents a therapeutic approach to treating schizoid personality disorder with the assistance of a nonhuman actor. [ABSTRACT FROM AUTHOR]

Brain microbleeds are increased in chronic kidney disease (CKD) and their presence increases risk of cognitive decline and stroke. We examined the interaction between CKD and brain microhemorrhages (the neuropathological substrate of microbleeds) in mouse and cell culture models and studied progression of microbleed burden on serial brain imaging from humans. Mouse studies: Two CKD models were investigated: adenine-induced tubulointerstitial nephritis and surgical 5/6 nephrectomy. Cell culture studies: bEnd.3 mouse brain endothelial cells were grown to confluence, and monolayer integrity was measured after exposure to 5-15% human uremic serum or increasing concentrations of urea. Human studies: Progression of brain microbleeds was evaluated on serial MRI from control, pre-dialysis CKD, and dialysis patients. Microhemorrhages were increased 2-2.5-fold in mice with CKD independent of higher blood pressure in the 5/6 nephrectomy model. IgG staining was increased in CKD animals, consistent with increased blood-brain barrier permeability. Incubation of bEnd.3 cells with uremic serum or elevated urea produced a dose-dependent drop in trans-endothelial electrical resistance. Elevated urea induced actin cytoskeleton derangements and decreased claudin-5 expression. In human subjects, prevalence of microbleeds was 50% in both CKD cohorts compared with 10% in age-matched controls. More patients in the dialysis cohort had increased microbleeds on follow-up MRI after 1.5 years. CKD disrupts the blood-brain barrier and increases brain microhemorrhages in mice and microbleeds in humans. Elevated urea alters the actin cytoskeleton and tight junction proteins in cultured endothelial cells, suggesting that these mechanisms explain (at least in part) the microhemorrhages and microbleeds observed in the animal and human studies.

Understanding the mitotic DNA damage response (DDR) is critical to our comprehension of cancer, premature aging and developmental disorders which are marked by DNA repair deficiencies. In this study we use a micro-focused laser to induce DNA damage in selected mitotic chromosomes to study the subsequent repair response. Our findings demonstrate that (1) mitotic cells are capable of DNA repair as evidenced by DNA synthesis at damage sites, (2) Repair is attenuated when DNA-PKcs and ATM are simultaneously compromised, (3) Laser damage may permit the observation of previously undetected DDR proteins when damage is elicited by other methods in mitosis, and (4) Twenty five percent of mitotic DNA-damaged cells undergo a subsequent mitosis. Together these findings suggest that mitotic DDR is more complex than previously thought and may involve factors from multiple repair pathways that are better understood in interphase.

Understanding the mitotic DNA damage response (DDR) is critical to our comprehension of cancer, premature aging and developmental disorders which are marked by DNA repair deficiencies. In this study we use a micro-focused laser to induce DNA damage in selected mitotic chromosomes to study the subsequent repair response. Our findings demonstrate that (1) mitotic cells are capable of DNA repair as evidenced by DNA synthesis at damage sites, (2) Repair is attenuated when DNA-PKcs and ATM are simultaneously compromised, (3) Laser damage may permit the observation of previously undetected DDR proteins when damage is elicited by other methods in mitosis, and (4) Twenty five percent of mitotic DNA-damaged cells undergo a subsequent mitosis. Together these findings suggest that mitotic DDR is more complex than previously thought and may involve factors from multiple repair pathways that are better understood in interphase.

Decentrare l’umano tramite forme narrative. La ‘chiusura impossibile’ del Pasticciaccio di Gadda e della Trilogia dell’Area X di VanderMeer Il presente articolo si pone l’obiettivo di individuare ed esaminare le caratteristiche narrative formali capaci di suggerire una concezione di vita come intricata matassa di incontri e relazioni fra esseri umani e non umani. Partendo dal presupposto che le innovazioni formali moderniste abbiano anticipato le narrative postumane contemporanee, l’articolo offre una comparazione neo-formalista fra Quer pasticciaccio brutto della via Merulana (1957) di Carlo Emilio Gadda e La trilogia dell’Area X (Annientamento, Autorità, Accettazione, 2015) di Jeff VanderMeer. In entrambe le opere sono identificabili due caratteristiche peculiari: la prima consiste in una comune impronta autoriale che segue un principio di accumulamento e complicazione manifestantesi in un accavallarsi di teorie non soddisfacenti e spesso incomplete, ma che comunque cercano di dare una soluzione o spiegazione ai misteri al centro dei rispettivi testi. La seconda corrisponde all’affidamento della narrazione ad una moltitudine di personaggi che si rivelano sempre parziali in quanto inabili a comprendere i fenomeni accaduti nella loro interezza. Infatti, i testi analizzati riescono a ridimensionare l’eccezionalità umana dimostrando che la realtà è sempre più complessa ed intrecciata di quanto sia umanamente comprensibile. Da questo ridimensionamento consegue l’impossibilità ad avere una conclusione che si possa definire finale.

Oral anticoagulants are a critical component of stroke prevention, but carry a risk of brain hemorrhage. These hemorrhagic complications tend to occur in elderly individuals, especially those with predisposing conditions such as cerebral amyloid angiopathy (CAA). Clinical evidence suggests that non-vitamin K antagonist oral anticoagulants are safer than traditional oral anticoagulants. We analyzed whether the anticoagulant dabigatran produces cerebral microhemorrhage (the pathological substrate of MRI-demonstrable cerebral microbleeds) or intracerebral hemorrhage in aged mice with and without hemorrhage-predisposing angiopathy. We studied aged (22 months old) Tg2576 (a model of CAA) and wild-type (WT) littermate mice. Mice received either dabigatran etexilate (DE) (Tg N = 7; WT N = 10) or vehicle (Tg N = 9; WT N = 7) by gavage for 4 weeks. Anticoagulation effects of DE were confirmed using thrombin time assay. No mice experienced intracerebral hemorrhage. Cerebral microhemorrhage analysis, performed using Prussian-blue and H&E staining, showed no significant change in either number or size of cerebral microhemorrhage in DE-treated animals. Analysis of biochemical parameters for endothelial activation (ICAM-1), blood-brain barrier disruption (IgG, claudin-5, fibrinogen), microglial activation (Iba-1), or astrocyte activation (GFAP) showed neither exacerbation nor protective effects of DE in either Tg2576 or WT mice. Our study provides histological and biochemical evidence that aged mice, with or without predisposing factors for brain hemorrhage, tolerate anticoagulation with dabigatran. The absence of dabigatran-induced intracerebral hemorrhage or increased frequency of acute microhemorrhage may provide some reassurance for its use in high-risk patient populations.

This paper examines Nathaniel Hawthorne’s ecophilosophy in “Rappaccini’s Daughter” by focusing on his various representations of the natural environment and the human relationship to it. It suggests that the story reflects his belief that nature is divinely arranged and that humanity should protect rather than manipulate its systems. From his perspective, the disruption of natural systems would not only cause the extinction of many animal species but also endanger human life and existence on earth. The paper further suggests that Hawthorne promotes a view of nature as a living organism whose entities possess souls and spirits. Their capacity to have feelings and emotions makes them entitled to moral respect and consideration. In its study of the author’s environmental values and ethics, the essay claims that Hawthorne advocates the idea that human beings do not occupy a privileged position in the universe and that they are not superior or more important than nonhumans. In contrast to the Biblical vision of humankind, he portrays humans as weak and flawed creatures that cannot attain divine perfection. For these reasons, the paper asserts that “Rappaccini’s Daughter” exhibits Hawthorne’s deep ecological awareness and underlines his stature as a pioneer of American literary environmentalism.

BACKGROUND:Cerebral microhemorrhages (CMH) are commonly found in the aging brain. CMH are also the neuropathological substrate of cerebral microbleeds (CMB), demonstrated on brain MRI. Recent studies demonstrate the importance of systemic inflammation in CMH development, but the relationships among inflammation, aging, and CMH development are not well-defined. In the current study, we hypothesized that the pathogenesis of inflammation-induced CMH in mice differs by age. METHODS:We studied young (3 months, n = 20) and old (18 months, n = 25) C57BL/6 mice injected with low-dose lipopolysaccharide (LPS; 1 mg/kg, i.p.) or saline at 0, 6, and 24 h. Seven days after the first LPS/saline injection, brains were harvested, sectioned, and stained with hematoxylin and eosin (H&E) and Prussian blue (PB) to estimate acute/fresh and sub-acute CMH development, respectively. The relationships between microglial/macrophage activation (ionized calcium-binding adapter molecule-1), astrocyte activation (glial fibrillary acidic protein), blood-brain barrier (BBB) disruption (brain immunoglobulin G), aging, and CMH development were examined using immunohistochemistry. RESULTS:Aging alone did not increase spontaneous H&E-positive CMH development but significantly increased the number, size, and total area of LPS-induced H&E-positive CMH in mice. LPS- and saline-treated aged mice had significantly larger PB-positive CMH compared with young mice, but the total area of PB-positive CMH was increased only in LPS-treated aged mice. Aged mice had significantly increased microglial/macrophage activation, which correlated with H&E- and PB-positive CMH development. Aged mice treated with LPS had significantly increased astrocyte activation and BBB disruption compared with young LPS-treated mice. CONCLUSIONS:Aging makes the brain more susceptible to inflammation-induced CMH in mice, and this increase in CMH with aging is associated with microglial/macrophage activation.

The paper discusses the more-than-human aspects of Charles S. Peirce's semiotic philosophy, contextualizes it within the history of ideas (Aristotle, the Medievals, Montaigne, Descartes) and examines its syntony with and differences from 21st century trends of cultural and philosophical studies (posthumanism, nonhuman studies, Object-Oriented-Ontology, and the more-than-human paradigm). [ABSTRACT FROM AUTHOR]

Peripheral endothelial cells are capable of erythrophagocytosis, but data on brain endothelial erythrophagocytosis are limited. We studied the relationship between brain endothelial erythrophagocytosis and cerebral microhemorrhage, the pathological substrate of MRI-demonstrable cerebral microbleeds. To demonstrate the erythrophagocytic capability of the brain endothelium, we studied the interactions between brain endothelial cells and red blood cells exposed to oxidative stress in vitro, and developed a new in vitro cerebral microbleeds model to study the subsequent passage of hemoglobin across the brain endothelial monolayer. Using multiple approaches, our results show marked brain endothelial erythrophagocytosis of red blood cells exposed to oxidative stress compared with control red blood cells in vitro. This brain endothelial erythrophagocytosis was accompanied by passage of hemoglobin across the brain endothelial monolayer with unaltered monolayer integrity. In vivo and confocal fluorescence microscopy studies confirmed the extravasation of RBC exposed to oxidative stress across brain endothelium. These findings, demonstrating erythrophagocytosis mediated by the brain endothelial monolayer and the subsequent passage of iron-rich hemoglobin in vitro and RBC in vivo, may have implications for elucidating mechanisms involved in the development of cerebral microbleeds that are not dependent on disruption of the microvasculature.

One of the most crucial features of new materialism is its critical stance towards the philosophies of linguistic or poststructuralist turn. It argues that too much emphasis on language has pushed the question of matter and materiality into oblivion, and by ascribing agential role to language it has foreclosed any possibility of approaching matter’s autopoietic capabilities. Since language has historically been thought to be the privileged domain of anthropos, new materialist thought is by right non-anthropocentric. But despite their claims to the contrary, it often appears that a significant section of new materialist scholarship finds itself mired into the humanist paradigm. This article is divided into two segments. The first segment explores how the nonhumanism that new materialist theses espouses functions through an additive approach that subscribes to metaphysical notions of human and nonhuman, leaving the constitution of these categories intact. The second part takes up the works of Vicki Kirby and delineates how a radical nonhumanism that renders the human/nonhuman binary untenable can still be possible from a new materialist premise.

BackgroundCerebral microbleeds (CMB) are MRI-demonstrable cerebral microhemorrhages (CMH) which commonly coexist with ischemic stroke. This creates a challenging therapeutic milieu, and a strategy that simultaneously protects the vessel wall and provides anti-thrombotic activity is an attractive potential approach. Phosphodiesterase 3A (PDE3A) inhibition is known to provide cerebral vessel wall protection combined with anti-thrombotic effects. As an initial step in the development of a therapy that simultaneously treats CMB and ischemic stroke, we hypothesized that inhibition of the PDE3A pathway is protective against CMH development.MethodsThe effect of PDE3A pathway inhibition was studied in the inflammation-induced and cerebral amyloid angiopathy (CAA)-associated mouse models of CMH. The PDE3A pathway was modulated using two approaches: genetic deletion of PDE3A and pharmacological inhibition of PDE3A by cilostazol. The effects of PDE3A pathway modulation on H&E- and Prussian blue (PB)-positive CMH development, BBB function (IgG, claudin-5, and fibrinogen), and neuroinflammation (ICAM-1, Iba-1, and GFAP) were investigated.ResultsRobust development of CMH in the inflammation-induced and CAA-associated spontaneous mouse models was observed. Inflammation-induced CMH were associated with markers of BBB dysfunction and inflammation, and CAA-associated spontaneous CMH were associated primarily with markers of neuroinflammation. Genetic deletion of the PDE3A gene did not alter BBB function, microglial activation, or CMH development, but significantly reduced endothelial and astrocyte activation in the inflammation-induced CMH mouse model. In the CAA-associated CMH mouse model, PDE3A modulation via pharmacological inhibition by cilostazol did not alter BBB function, neuroinflammation, or CMH development.ConclusionsModulation of the PDE3A pathway, either by genetic deletion or pharmacological inhibition, does not alter CMH development in an inflammation-induced or in a CAA-associated mouse model of CMH. The role of microglial activation and BBB injury in CMH development warrants further investigation.

This paper seeks to situate the anxieties engendered by the COVID-19 pandemic within the framework of the Anthropocene to analyse the multi-faceted ramifications of human and nonhuman interaction. By connecting this ongoing global crisis of human health with the politics of climate change, it attempts to read the forgotten agency of the nonhuman microbe in the light of the rude disruption of the traditional understandings of biopolitics (where bare life has taken centre stage) and the difficulties it has brought in bridging the rift between abstract and concrete information, leading to the scapegoating of victims. It ends with the suggestion of preparation for greener futures by imagining human health within planetary health instead of an anxious wait for a return to pre-pandemic times. [ABSTRACT FROM AUTHOR]

Research on the resilience of young people who were raised by substance-abusing caregivers is limited. This study aims to explore the internal interactional processes between nonhuman systems and young adults raised by alcohol-abusing caregivers in Lesotho. Multiple in-depth interviews were conducted and a draw-and-write technique applied with 15 university students, six of whom described having interacted with diverse nonhuman systems in their environment. A grounded theory analysis generated two themes: (1) interacting with empowering messages from non-present writers (through songs and books) and inspirational speakers (through videos) and (2) interacting with imaginary friends and inanimate objects (dolls and tattoos) in order to enhance their resilience. Van Breda’s interactional resilience approach, developed from person-in-the-environment perspective, and Margaret Archer’s theory of agency were found to be useful in interpreting the findings. The implications of the study include the need for social workers’ greater focus on young people’s interactions with nonhuman systems for resilience building.

The research’s main objective is to explore and encourage modes of architectural practice that can foster multispecies co-living to reduce biodiversity loss and increase the quality of life for both human and nonhuman inhabitants of architecture. This is achieved through conceptual discussions, comprehensive architectural case studies and work-based design explorations that support cross-species co-living in the context of Eastern Norway (Østlandet)—a geographical region of south-eastern Norway consisting of the counties Vestfold, Telemark, Viken, Oslo and Innlandet. A pluralistic method builds on analytical, critical and work-based explorative studies consisting of two parts: (a) historical and contemporary case studies in Norway that support modes of cross-species co-living and (b) design explorations by the second author investigating the operational potential of kindness in architecture. The notion of kindness in this research is built upon an understanding of the amalgam of concepts: solidarity, kinship and being kind, explained in the article’s introduction. The potential for designing with and for nonhumans to reinvigorate modes of co-living and support existing habitats is investigated, focusing on the ways three bird species relate to a specific building in Eastern Norway due to their habitat needs in the region: Cyanistes caeruleus, Eurasian blue tit (blåmeis in Norwegian); Passer montanus, Eurasian tree sparrow (pilfink in Norwegian); and Delichon urbicum, northern or common house martin (taksvale in Norwegian). The research contributes to ongoing discussions within architectural discourse regarding multispecies inhabitation and architecture’s role in the current biodiversity crisis and provides insight into both historical and contemporary/ongoing design solutions for multispecies co-living.

This article proposes a posthumanist reading of Joyce Carol Oates's "Poe Posthumous; or, The Light-House," and argues that posthumanism provides human beings with a new way of living. In the story, the narrator comes to the Light-House at Viña de Mar to participate in a scientific experiment. As an "exemplary specimen of Homo sapiens," the narrator endeavours to preserve human knowledge, which symbolises his superiority over nonhuman species. However, on becoming further involved in nature, he gradually abandons his anthropocentric thought, and learns to live with other species. The posthumanist thought finds its full expression in the symbiotic coexistence of multiple species and culminates in the narrator's cross-species marriage to a female Cyclophagus. The juxtaposition of the decentring of anthropocentrism with the ascent of nonhuman agents highlights the posthumanist coexistence of humans and nonhumans. [ABSTRACT FROM AUTHOR]

The argument behind our introductory essay, as well as behind our special issue of the Metacritic Journal for Comparative Studies and Theory, is that the human can emerge as part of the texture of the world only by being reminded of and by objects, that is, only by engaging in a dialogue with them, by recognising both their strangeness and their familiarity, by conferring upon them the distinction of containing the traces and archives of individual and collective history. We examine the turn towards things enacted by recent critical and artistic trends as a complex amalgam of de-anthropocentrising modes recalibrating experience under the pressure of imminent extinction (of humans, animals, inanimate objects) which produces a new imperative of togetherness by excavating the memory of our shared materiality.

This paper builds on biomedical and anthropological discourses of microbial agency to explore the important opportunities this discourse offers medicine, politics, anthropology, and patients. “Borrelia burgdorferi”, often termed “the Great Imitator”, is an ideal candidate for this discussion as it reveals how difficult it is to speak about Lyme disease without engaging with microbial agency. Based on 12-months research with Lyme disease patients and clinicians in Scotland, this paper offers a social rendering of the bacteria that reveals epistemologies of illness not available in medical accounts: the impact of social and psychological symptoms such as body dysmorphia, depression, shame, post-traumatic stress disorder, and suicide-related deaths on patients’ illness narratives. Divorcing agency from the bacteria silences these important patient narratives with the consequence of a limited medical and social understanding of the signification of Lyme disease and the holistic methods needed for treatment. This paper furthermore argues that the inclusion of patient worldings of Borrelia acting in the medical renderings offers a democratic determination of what the illness is. Finally, building on Giraldo Herrera and Cadena, I argue for a decolonization of Borrelia, exploring how the pluriverse both takes the epistemologies of patients seriously and reveals medical equivocation.

We describe the general occurrence in animal cells of elastic components ("tethers") that connect individual chromosomes moving to opposite poles during anaphase. Tethers, originally described in crane-fly spermatocytes, exert force on chromosome arms opposite to the direction the anaphase chromosomes move. We show that they exist in a broad range of animal cells. Thus tethers are previously unrecognised components of general mitotic mechanisms that exert force on chromosomes and they need to be accounted for in general models of mitosis in terms of forces on chromosomes and in terms of what their roles might be.

Chronic kidney disease (CKD) is an independent risk factor for the development of cerebrovascular disease, particularly small vessel disease which can manifest in a variety of phenotypes ranging from lacunes to microbleeds. Small vessel disease likely contributes to cognitive dysfunction in the CKD population. Non-traditional risk factors for vascular injury in uremia include loss of calcification inhibitors, hyperphosphatemia, increased blood pressure variability, elastinolysis, platelet dysfunction, and chronic inflammation. In this review, we discuss the putative pathways by which these mechanisms may promote cerebrovascular disease and thus increase risk of future stroke in CKD patients.

BackgroundCerebral microhemorrhages (CMH) are tiny deposits of blood degradation products in the brain and are pathological substrates of cerebral microbleeds. The existing CMH animal models are β-amyloid-, hypoxic brain injury-, or hypertension-induced. Recent evidence shows that CMH develop independently of hypoxic brain injury, hypertension, or amyloid deposition and CMH are associated with normal aging, sepsis, and neurodegenerative conditions. One common factor among the above pathologies is inflammation, and recent clinical studies show a link between systemic inflammation and CMH. Hence, we hypothesize that inflammation induces CMH development and thus, lipopolysaccharide (LPS)-induced CMH may be an appropriate model to study cerebral microbleeds.MethodsAdult C57BL/6 mice were injected with LPS (3 or 1 mg/kg, i.p.) or saline at 0, 6, and 24 h. At 2 or 7 days after the first injection, brains were harvested. Hematoxylin and eosin (H&E) and Prussian blue (PB) were used to stain fresh (acute) hemorrhages and hemosiderin (sub-acute) hemorrhages, respectively. Brain tissue ICAM-1, IgG, Iba1, and GFAP immunohistochemistry were used to examine endothelium activation, blood-brain barrier (BBB) disruption, and neuroinflammation. MRI and fluorescence microscopy were used to further confirm CMH development in this model.ResultsLPS-treated mice developed H&E-positive (at 2 days) and PB-positive (at 7 days) CMH. No surface and negligible H&E-positive CMH were observed in saline-treated mice (n = 12). LPS (3 mg/kg; n = 10) produced significantly higher number, size, and area of H&E-positive CMH at 2 days. LPS (1 mg/kg; n = 9) produced robust development of PB-positive CMH at 7 days, with significantly higher number and area compared with saline (n = 9)-treated mice. CMH showed the highest distribution in the cerebellum followed by the sub-cortex and cortex. LPS-induced CMH were predominantly adjacent to cerebral capillaries, and CMH load was associated with indices of brain endothelium activation, BBB disruption, and neuroinflammation. Fluorescence microscopy confirmed the extravasation of red blood cells into the brain parenchyma, and MRI demonstrated the presence of cerebral microbleeds.ConclusionsLPS produced rapid and robust development of H&E-positive (at 2 days) and PB-positive (at 7 days) CMH. The ease of development of both H&E- and PB-positive CMH makes the LPS-induced mouse model suitable to study inflammation-induced CMH.

Loneliness and isolation were two factors introduced as “effective measures” during the COVID-19 crisis. The lockdown exacerbated loneliness among those already suffering from acute illnesses. In this context, a rereading of the film Lars and the Real Girl by Craig Gillespie is particularly relevant as it offers novel perspectives on loneliness. The interplay between Lars’s desire to be in a compassionate relationship and the fear of meeting and socializing is comparable to what was witnessed across the coronavirus-afflicted world. This paper explores the potential for understanding delusion caused by traumatic experiences as a form of communication rather than a mental disorder. The film explains how a silicone sex doll functions as a medium between the lonesome Lars and society in resolving the trauma. The paper focuses on the infantile nature of humans and uses infantilism in a conducive manner to understand anthropomorphism for bridging the gap between a lonely/delusional person and society while drawing examples from the film. The introduction of a nonhuman actor—an anatomically correct doll—becomes an opportunity for a traumatized person such as Lars to know himself well and gradually open up to socializing. As he moves from external to threshold en-rolling, followed by internal en-rolling, it indicates his opening up to communication as he moves from language to lalangue and creates his world with the doll. This film presents a therapeutic approach to treating schizoid personality disorder with the assistance of a nonhuman actor.

Salmonellosis ranks among the most frequently reported zoonosis worldwide and is often associated with foodborne outbreaks. Since the 1950s, the distribution of Salmonella serotypes in Sao Paulo State, Brazil, has been documented and periodically reported. In this study, we updated the data on the distribution of Salmonella serotypes received in our reference laboratory, isolated from human infections and nonhuman sources, from 2004 to 2020. In that period, a total of 9,014 Salmonella isolates were analyzed, of which 3,553 (39.4%) were recovered from human samples, mainly of stool (65%) and blood (25.6%), and 5,461 (60.6%) were isolated from nonhuman origins, such as animals (47.2%), food (27.7%) and animal environments (18.6%). In human isolates, a total of 104 serotypes were identified and the most frequent ones were Enteritidis, Typhimurium, S. I. 4,[5],12:i:-, Dublin and Typhi. A consistent reduction of the Enteritidis proportion was observed over the years. Among the 156 serotypes identified in isolates with nonhuman origins, Enteritidis, Mbandaka, Typhimurium, Agona and Anatum were ranked as the top five Salmonella serotypes; in more recent years, S. Heidelberg has increased in frequency. Although with different proportions, the top 10 prevalent serotypes were identified in both human and nonhuman origins, underscoring the role of animals, food products and environment as reservoirs of Salmonella with potential to cause human salmonellosis. [ABSTRACT FROM AUTHOR]

This article delves into the problem of nonhuman subjectivity in two literary texts: Jessica Grant's Come, Thou Tortoise with the first-person tortoise narration, and Colin McAdam's A Beautiful Truth that employs the collective primate narrator. While nonhumans cannot actively participate in the act of creation of the text, their presence in the story, arranged by the author, conveys multiple meanings. Considerations of the narrative techniques are critical for negotiating the relevance of nonhuman actors. I argue that although each author finds different methods of giving voice to nonhumans and both ensure practical significance of animal particularity, nonhuman subjectivity should not be perceived as a fixed value of the presented literary texts. [ABSTRACT FROM AUTHOR]

Yoko Tawada, an author writing in both Japanese and German, is what critics call an exophonic writer, that is, a writer who uses a language other than one's mother tongue for creative purposes. Writing from a foreign point of view is part of Tawada's interest in acquiring perceptions of otherness both linguistically and culturally. We might apply Tawada's exophonic writing when entering animal worlds by creating what Frederike Middelhoff terms 'literary auto-zoographies'. Tawada's novel Memoirs of a Polar Bear contains three generations of polar bear narratives: two circus performers and one zoo inhabitant. The text takes a postmodern metafictional approach to problems that arise in speaking for the animal other, a subject under much discussion in Animal Studies scholarship today. My article examines each of the three characters and their corresponding narrative modes. First, the grandmother polar bear writes a first-person autobiography of her life as a performer; in doing so, Tawada combines fiction and nonfiction to deconstruct the bear character's identity thus resulting in what might be called a more authentic animal autobiography. Second, the article focuses on Tawada's fascination with translation through the human-animal shared spaces between Tosca (the daughter of the unnamed grandmother polar bear character) and her human trainer. Lastly, the article examines the grandson, Knut, as an example of the current humanimal subject of ecopoetics with an emphasis on Knut as an environmental figure. [ABSTRACT FROM AUTHOR]

Research on the resilience of young people who were raised by substance-abusing caregivers is limited. This study aims to explore the internal interactional processes between nonhuman systems and young adults raised by alcohol-abusing caregivers in Lesotho. Multiple in-depth interviews were conducted and a draw-and-write technique applied with 15 university students, six of whom described having interacted with diverse nonhuman systems in their environment. A grounded theory analysis generated two themes: (1) interacting with empowering messages from non-present writers (through songs and books) and inspirational speakers (through videos) and (2) interacting with imaginary friends and inanimate objects (dolls and tattoos) in order to enhance their resilience. Van Breda's interactional resilience approach, developed from person-in-the-environment perspective, and Margaret Archer's theory of agency were found to be useful in interpreting the findings. The implications of the study include the need for social workers' greater focus on young people's interactions with nonhuman systems for resilience building. [ABSTRACT FROM AUTHOR]

Axonal injury and stress have long been thought to play a pathogenic role in a variety of neurodegenerative diseases. However, a model for studying single-cell axonal injury in mammalian cells and the processes of repair has not been established. The purpose of this study was to examine the response of neuronal growth cones to laser-induced axonal damage in cultures of embryonic rat hippocampal neurons and induced pluripotent stem cell (iPSC) derived human neurons. A 532-nm pulsed [Formula: see text] picosecond laser was focused to a diffraction limited spot at a precise location on an axon using a laser energy/power that did not rupture the cell membrane (subaxotomy). Subsequent time series images were taken to follow axonal recovery and growth cone dynamics. After laser subaxotomy, axons thinned at the damage site and initiated a dynamic cytoskeletal remodeling process to restore axonal thickness. The growth cone was observed to play a role in the repair process in both hippocampal and iPSC-derived neurons. Immunofluorescence staining confirmed structural tubulin damage and revealed initial phases of actin-based cytoskeletal remodeling at the damage site. The results of this study indicate that there is a repeatable and cross-species repair response of axons and growth cones after laser-induced damage.