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2 results on '"zymographic analysis"'

1. Zymographic analysis of lipolytical enzymes of subcellular Vibrio cholerae fractions of O1 and O139 serogroups

Author

S. N. Kozlov, V. B. Nikolaev, E. Y. Markov, L. Y. Urbanovich, and S. K. Mitkeeva

Subjects
vibrio cholerae, zymographic analysis, subcellular fraction, lipolytical enzyme, substrate electrophoresis, Science
Abstract

Zymographic analysis showed that all subcellular fractions of the serogroups possessed different degree of lipolytical activity. Maximal hydrolysis in the radial enzyme-diffusion reaction in agarose gel was observed in Tween-20, Tween-80, Triton Х-305 and Triton Х-405. Total hydrolase activity of urea extracts in Tween-20, Tween-80, Triton Х-405, Span-85 excelled those in the preparations of outer membranes. Maximal lipolytical activity in regard to the Tweens was shown by the preparations of urea extracts prepared from the nontoxigenic strains of O1 serogroups, while in relation to the Tritons - preparations of urea extracts from the toxigenic strains (p < 0,05).

Published
2016
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2. ZYMOGRAPHIC ANALYSIS OF LIPOLYTICAL ENZYMES OF SUBCELLULAR VIBRIO CHOLERAE FRACTIONS OF O1 AND O139 SEROGROUPS

Author

Svetlana Mitkeeva, null ��������, Lyudmila Urbanovich, null ���������, Stanislav Kozlov, null ������, Valeriy Nikolaev, and Evgeniy Markov

Subjects
chemistry.chemical_classification, integumentary system, Science, vibrio cholerae, medicine.disease_cause, substrate electrophoresis, Microbiology, subcellular fraction, Enzyme, chemistry, lipolytical enzyme, Vibrio cholerae, zymographic analysis, medicine
Abstract

Zymographic analysis showed that all subcellular fractions of the serogroups possessed different degree of lipolytical activity. Maximal hydrolysis in the radial enzyme-diffusion reaction in agarose gel was observed in Tween-20, Tween-80, Triton �-305 and Triton �- 405. Total hydrolase activity of urea extracts in Tween-20, Tween-80, Triton �- 405, Span-85 excelled those in the preparations of outer membranes. Maximal lipolytical activity in regard to the Tweens was shown by the preparations of urea extracts prepared from the nontoxigenic strains of O1 serogroups, while in relation to the Tritons � preparations of urea extracts from the toxigenic strains (p < 0,05).

Published
2016
Full Text
View/download PDF

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