1. The Growth of Transgenic Tobacco Plants with Estradiol-Induced Expression of Tomato Xyloglucan Endotransglucosylase Gene <italic>tXET-B2</italic> under Stress Conditions.
- Author
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Berezhneva, Z. A., Mikhaylova, E. V., and Kuluev, B. R.
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TRANSGENIC plants , *ESTRADIOL , *XYLOGLUCANS , *ABIOTIC stress , *GENETIC regulation - Abstract
The role of xyloglucan endotransglycosylases in the regulation and promotion of plant growth in response to such widespread stress factors as drought, salinization, and hypothermia remains poorly understood. The
tXET-B2 (SlXTH10 ) gene encodes one of the xyloglucan endotransglycosylases of tomato, which is most closely related in the nucleotide sequence to theAtXTH15 andAtXTH16 genes of Arabidopsis thaliana. At present, the specific functions of the tXET-B2 gene, as well as of its homologs,AtXTH15 andAtXTH16 , remain obscure. To study the role oftXET-B2 in the regulation of growth and adaptation to abiotic stress factors, transgenic tobacco plants with estradiol-inducible expression of the tomatotXET-B2 gene were generated. Overexpression of this gene promoted tobacco root growth in a medium containing 50 mM NaCl. Under drought conditions, exogenous treatment with estradiol resulted in a considerable increase in fresh and dry weight in many of the studied transgenic lines. Under normal conditions, as well as under salinization and hypothermia stress, such positive effect was detected only for some transgenic lines. The obtained data point to the possibility of using genetically engineered constructs of thetXET-B2 gene to correct growth parameters of transgenic plants under the influence of stress factors. [ABSTRACT FROM AUTHOR]- Published
- 2018
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