Search

Cover cropping plays a key role in the maintenance of arable soil health and the enhancement of agroecosystem services. However, our understanding of how cover crop management impacts soil microbial communities and how these interactions might affect soil nutrient cycling is still limited. Here, we studied the impact of four cover crop mixtures varying in species richness and functional diversity, three cover crop termination strategies (i.e., frost, rolling, and glyphosate) and two levels of irrigation at the cover crop sowing on soil nitrogen and carbon dynamics, soil microbial diversity, and structure as well as the abundance of total bacteria, archaea, and N -cycling microbial guilds. We found that total nitrogen and soil organic carbon were higher when cover crops were killed by frost compared to rolling and glyphosate termination treatments, while cover crop biomass was positively correlated to soil carbon and C:N ratio. Modifications of soil properties due to cover crop management rather than the composition of cover crop mixtures were related to changes in the abundance of ammonia oxidizers and denitrifiers, while there was no effect on the total bacterial abundance. Unraveling the underlying processes by which cover crop management shapes soil physico-chemical properties and bacterial communities is of importance to help selecting optimized agricultural practices for sustainable farming systems. [ABSTRACT FROM AUTHOR]

Climate change models predict shifts in the frequency and magnitude of rain events (precipitation patterns). We studied how precipitation history shapes microbial community responses to rewetting and how these effects depend on N status. Twelve weeks of contrasting precipitation and N input left a legacy effect by shaping present (DNA-based) and potentially active (rRNA-based) bacterial and fungal communities. This legacy effect determined the microbial response to rewetting, as demonstrated by differences in the post-wet potentially active bacterial and fungal communities as well as the flux of soil-emitted CO 2 over a 29-h period. Despite contrasting effects of precipitation and N input history on fungal:bacterial ratio and microbial community composition, the timing of the potentially active bacterial and fungal response to rewetting was not altered. Thus, regardless of precipitation or N input history, potentially active bacteria responded with a small shift in community composition within 1 h of rewetting but did not change further for the remaining 28 h analyzed. The potentially active fungi did not respond to rewetting within 29 h. Even though more extreme fluctuations in soil moisture changed soil microbial community composition, the short-term response of microbial communities to rewetting was conserved. Soil CO 2 efflux upon rewetting was higher from systems with a history of frequent precipitation and underline the role of plants in drying-rewetting processes. We suggest that shifts in the fungal:bacterial ratio, as well as N-cycling potentials, may have consequences on food web stability and soil biogeochemical cycling. • Precipitation and N input legacy shaped soil bacterial and fungal communities. • Soils with a frequent precipitation legacy emitted more CO 2 upon rewetting. • The potentially active bacterial community shifted regardless of legacy. • Fungal communities did not respond to rewetting (assessed over 29-h). [ABSTRACT FROM AUTHOR]