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Abstract Bioaccessibility of particle-bound hydrophobic organic contaminants and related particle size effects are significant for assessing the potential human health risk via inhalation exposure, but have not been clearly evaluated. To fill this knowledge gap, the present study developed an in vitro method to estimate the inhalation bioaccessibility of particulate polycyclic aromatic hydrocarbons (PAHs) using simulated human lung fluids, i.e. , a modified Gamble's solution (MGS) and artificial lysosomal fluid (ALF) with Tenax as the absorption media. Assay parameters, namely incubation time (10 d) and influence of filter use, were optimized for establishing the in vitro method. The results showed that the bioaccessibility of PAHs increased with increasing particle size, but other factors, such as total organic carbon and chemical hydrophobicity, also played a large role in the fate of these compounds. The results from this portion of the present study were then used to evaluate human health risk, which showed that the risk of these particle-bound PAHs by incorporating size-dependent PAHs bioaccessibility and deposition efficiency in the human respiratory tract into inhalation exposure risk calculations was reduced by >90% when compared to using total concentration. This suggested that the inhalation bioaccessibility and deposition efficiency of hydrophobic organic chemicals should be included in human health risk assessment. Graphical abstract Unlabelled Image Highlights • An in vitro method was developed for evaluating the IBAF of particle-bound HOCs. • The inhalation bioaccessibility of PAHs decreased with increasing hydrophobicity. • The IBAF of particle-bound HOCs is significant to human health risk assessment. [ABSTRACT FROM AUTHOR]

Human health concerns are rising with polybrominated diphenyl ethers' (PBDEs) analogues, methoxylated and hydroxylated PBDEs (MeO-PBDEs and OH-PBDEs), due to their occurrences in foods and greater potential toxicological effects than PBDEs. While the oral bioaccessibilities (BA%) of PBDEs in foods are available, such information on MeO-PBDEs and OH-PBDEs, and the effects of cooking on them have not been adequately addressed. The present study was conducted with fish and chicken egg as typical foods to assess the bioaccessibility (BA%) of PBDEs, MeO-PBDEs, and OH-PBDEs using the colon extended physiologically based extraction test and examine the effects of cooking processes (boiling, frying, and steaming) on them. The results showed that thermal degradation or transformation of the target compounds did not occur during boiling and frying of fish. The BA% of individual PBDEs, MeO-PBDEs, and OH-PBDEs were 20–51% for boiled fish, 11–20% for pan-fried fish, 15–77% for steamed egg, and 42–68% for pan-fried egg. Cooking decreased the BA% of all target compounds in fish due to protein denaturation. However, the BA% of OH-PBDEs in pan-fried egg were greater than those in steamed egg. In addition, the substituent groups of CH 3 O- and OH- did not pose any effects on the BA% of BDE-47 in fish, but OH-group decreased its BA% in egg. These findings suggested that MeO-PBDEs and OH-PBDEs exhibited the similar oral BA% in fish to PBDEs, but the underlying mechanism for the effects of cooking on BA% of OH-PBDEs in egg needs to be further investigated. Unlabelled Image • MeO- and OH-PBDEs exhibited the similar bioaccessibility in fish and egg to PBDEs. • Cooking decreased the bioaccessibility of PBDEs, MeO-PBDEs, and OH-PBDEs in fish. • PBDEs and MeO- and OH-PBDEs were more bioaccessible in fried egg than fried fish. [ABSTRACT FROM AUTHOR]