9 results on '"Sears, Cynthia"'
Search Results
2. Bacteroides fragilis Toxin Coordinates a Pro-carcinogenic Inflammatory Cascade via Targeting of Colonic Epithelial Cells
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Chung, Liam, Orberg, Erik Thiele, Geis, Abby L, Chan, June L, Fu, Kai, Shields, Christina E DeStefano, Dejea, Christine M, Fathi, Payam, Chen, Jie, Finard, Benjamin B, Tam, Ada J, McAllister, Florencia, Fan, Hongni, Wu, Xinqun, Ganguly, Sudipto, Lebid, Andriana, Metz, Paul, Van Meerbeke, Sara W, Huso, David L, Wick, Elizabeth C, Pardoll, Drew M, Wan, Fengyi, Wu, Shaoguang, Sears, Cynthia L, and Housseau, Franck
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Biochemistry and Cell Biology ,Biomedical and Clinical Sciences ,Biological Sciences ,Digestive Diseases ,Colo-Rectal Cancer ,Cancer ,Adenomatous Polyposis Coli Protein ,Animals ,Bacterial Toxins ,Bacteroides fragilis ,Carcinogenesis ,Cell Line ,Tumor ,Colon ,Colorectal Neoplasms ,Enzyme Activation ,Epithelial Cells ,Female ,Gene Deletion ,HT29 Cells ,Humans ,Inflammation ,Interleukin-17 ,Male ,Metalloendopeptidases ,Mice ,Mice ,Inbred C57BL ,Mice ,Knockout ,Myeloid Cells ,Receptors ,Interleukin-17 ,Receptors ,Interleukin-8B ,STAT3 Transcription Factor ,Transcription Factor RelA ,Nf-κB ,STAT-3 ,colorectal cancer ,inflammation ,mucosal immunology ,myeloid cells ,Microbiology ,Medical Microbiology ,Immunology ,Biochemistry and cell biology ,Medical microbiology - Abstract
Pro-carcinogenic bacteria have the potential to initiate and/or promote colon cancer, in part via immune mechanisms that are incompletely understood. Using ApcMin mice colonized with the human pathobiont enterotoxigenic Bacteroides fragilis (ETBF) as a model of microbe-induced colon tumorigenesis, we show that the Bacteroides fragilis toxin (BFT) triggers a pro-carcinogenic, multi-step inflammatory cascade requiring IL-17R, NF-κB, and Stat3 signaling in colonic epithelial cells (CECs). Although necessary, Stat3 activation in CECs is not sufficient to trigger ETBF colon tumorigenesis. Notably, IL-17-dependent NF-κB activation in CECs induces a proximal to distal mucosal gradient of C-X-C chemokines, including CXCL1, that mediates the recruitment of CXCR2-expressing polymorphonuclear immature myeloid cells with parallel onset of ETBF-mediated distal colon tumorigenesis. Thus, BFT induces a pro-carcinogenic signaling relay from the CEC to a mucosal Th17 response that results in selective NF-κB activation in distal colon CECs, which collectively triggers myeloid-cell-dependent distal colon tumorigenesis.
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- 2018
3. Metabolism Links Bacterial Biofilms and Colon Carcinogenesis
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Johnson, Caroline H, Dejea, Christine M, Edler, David, Hoang, Linh T, Santidrian, Antonio F, Felding, Brunhilde H, Ivanisevic, Julijana, Cho, Kevin, Wick, Elizabeth C, Hechenbleikner, Elizabeth M, Uritboonthai, Winnie, Goetz, Laura, Casero, Robert A, Pardoll, Drew M, White, James R, Patti, Gary J, Sears, Cynthia L, and Siuzdak, Gary
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Biological Sciences ,Biomedical and Clinical Sciences ,Microbiology ,Medical Biochemistry and Metabolomics ,Oncology and Carcinogenesis ,Digestive Diseases ,Cancer ,Colo-Rectal Cancer ,2.1 Biological and endogenous factors ,Aetiology ,Bacteria ,Bacterial Physiological Phenomena ,Biofilms ,Colonic Neoplasms ,Female ,Humans ,Male ,Spermine ,Biochemistry and Cell Biology ,Endocrinology & Metabolism ,Biochemistry and cell biology ,Medical biochemistry and metabolomics - Abstract
Bacterial biofilms in the colon alter the host tissue microenvironment. A role for biofilms in colon cancer metabolism has been suggested but to date has not been evaluated. Using metabolomics, we investigated the metabolic influence that microbial biofilms have on colon tissues and the related occurrence of cancer. Patient-matched colon cancers and histologically normal tissues, with or without biofilms, were examined. We show the upregulation of polyamine metabolites in tissues from cancer hosts with significant enhancement of N(1), N(12)-diacetylspermine in both biofilm-positive cancer and normal tissues. Antibiotic treatment, which cleared biofilms, decreased N(1), N(12)-diacetylspermine levels to those seen in biofilm-negative tissues, indicating that host cancer and bacterial biofilm structures contribute to the polyamine metabolite pool. These results show that colonic mucosal biofilms alter the cancer metabolome to produce a regulator of cellular proliferation and colon cancer growth potentially affecting cancer development and progression.
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- 2015
4. The Role of the Gut Microbiome in Cancer: A Review, With Special Focus on Colorectal Neoplasia and Clostridioides difficile.
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Anderson, Sean M and Sears, Cynthia L
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TUMOR risk factors , *HOMEOSTASIS , *LIFESTYLES , *GUT microbiome , *CLOSTRIDIOIDES difficile , *BIOFILMS , *IMMUNE system , *COLORECTAL cancer , *CLOSTRIDIUM diseases , *BACTERIAL toxins , *INTESTINAL mucosa , *METABOLITES , *DISEASE risk factors , *DISEASE complications ,TUMOR prevention - Abstract
The gut microbiome has coevolved with humans to aid in physiologic functions and prevent disease. An increasing prevalence of gut dysbiosis in modern society exists and has strong linkages to multiple disease processes common in the developed world. Mechanisms for microbiome-human interactions that impact host homeostasis include bacterial metabolite/toxin production, biofilm formation with mucous layer infiltration, and host immune system modulation. Most of this crosstalk occurs at the epithelial layer of the gut, and as such the role of these interactions in the induction of colorectal cancer—a highly prevalent disease globally and one undergoing significant epidemiologic shifts—is under increasing scrutiny. Although multiple individual gut bacteria have been hypothesized as possible driver organisms in the oncogenic process, no bacterium has been definitively identified as a causal agent of colorectal cancer, suggesting that host lifestyle factors, microbiome community interactions, and the mucosal and/or systemic immune response may play a critical role in the process. Recent evidence has emerged implicating the ubiquitous human pathogen Clostridioides difficile as a possible promoter of colorectal cancer through chronic toxin-mediated cellular changes. Although much remains to be defined regarding the natural history of infections caused by this pathogen and its potential for oncogenesis, it provides a strong model for the role of both individual bacteria and of the gut microbial community as a whole in the development of colorectal cancer. The gut microbiome can play a crucial role in the development and prevention of human cancers through a variety of individual bacterial and broader community mechanisms. Emerging evidence suggests that Clostridioides difficile could be an important contributor to this balance. [ABSTRACT FROM AUTHOR]
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- 2023
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5. Subtle, persistent shaping of the gut microbiome by host genes: A critical determinant of host biology.
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Dzierozynski, Lindsey, Queen, Jessica, and Sears, Cynthia L.
- Abstract
Although environmental impacts on the host microbiome have been well studied, it is less certain whether and how host genetics impact the microbiome. This commentary discusses current literature supporting host genetic influences on resident species and pathogenic microbes. Mechanistic experimental studies are warranted to understand host gene-microbiome interplay. [ABSTRACT FROM AUTHOR]
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- 2023
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6. Polyamine catabolism contributes to enterotoxigenic Bacteroides fragilis-induced colon tumorigenesis
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Goodwin, Andrew C., Shields, Christina E. Destefano, Wu, Shaoguang, Huso, David L., Wu, XinQun, Murray-Stewart, Tracy R., Hacker-Prietz, Amy, Rabizadeh, Shervin, Woster, Patrick M., Sears, Cynthia L., and Casero,, Robert A.
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- 2011
7. Perspective: Alpha-Bugs, Their Microbial Partners, and the Link to Colon Cancer
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Sears, Cynthia L. and Pardoll, Drew M.
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- 2011
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8. 2845. Oral Antibiotic Use and Risk of Colorectal Cancer in the UK, 1989–2012: A Matched Case–Control Study.
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Zhang, Jiajia, Haines, Charles, Watson, Alastair, Hart, Andrew, Platt, Mary Jane, Pardoll, Drew, Cosgrove, Sara E, Gebo, Kelly, and Sears, Cynthia
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COLORECTAL cancer ,RECTAL cancer ,CASE-control method ,ANTIBIOTICS ,TETRACYCLINES ,HEREDITARY nonpolyposis colorectal cancer - Abstract
Background Microbiome dysbiosis predisposes to colorectal cancer (CRC), but a population-based study of oral antibiotic exposure and CRC risk is lacking. Methods A matched case–control study (incident CRC cases and up to 5 matched controls) was conducted in the Clinical Practice Research Datalink (CPRD; 1989–2012). The CRPD is validated as 92% and 99% sensitive and specific for CRC detection (98% PPV). Antibiotic exposure [categorical and continuous terms (spline)] was investigated for risk pattern, stratified by tumor location, using conditional logistic regression and adjusting for known confounders. Results In total, 28,980 CRC cases and 137,077 controls were identified. Oral antibiotic use increased risk of colon cancer in a dose-dependent fashion (P
trend < 0.001), but effects differed by anatomic location. Colon cancer risk was greatest in the proximal colon and with antibiotics with anti-anaerobic activity (Figure 1). In contrast, an inverse association was detected between antibiotic use and rectal cancers (Ptrend = 0.003), particularly with length of antibiotic exposure >60 days (adjusted odds ratio [AOR], 0.85, 95% CI 0.79–0.93) when compared with no antibiotic exposure. Nonlinearity models showed significantly increased colon cancer risk after minimal antibiotic use, but decreased rectum cancer risk with cumulative use of over 30 days (Figure 2). Penicillins, particularly ampicillin/amoxicillin, increased risk of colon cancer (AOR,1.09, [1.05–1.13]) whereas tetracyclines reduced risk for rectal cancer (AOR, 0.90, [0.84–0.97]). Significant interactions were detected between antibiotic use and tumor location (colon vs. rectum, Pinteraction < 0.001). The antibiotic-cancer association was found for antibiotic exposure occurring >10 years before diagnosis (AOR, 1.17, [1.06–1.31]). Conclusion We conclude that oral antibiotic use associates with increased colon cancer risk, particularly in the right colon, but a reduced risk for rectal cancer. This effect heterogeneity suggests unabsorbed antibiotics impact gut microbiota in the right colon to enhance carcinogenesis whereas antibiotic anti-inflammatory or anti-proliferative actions may yield an inverse effect on carcinogenesis in the rectum. Disclosures Sara E. Cosgrove, MD, MS, Basilea: Consultant; Theravance: Consultant. [ABSTRACT FROM AUTHOR]- Published
- 2019
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9. 2611. Enterotoxigenic Bacteroides fragilis Alters the Genome of Colon Epithelial Cells.
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Allen, Jawara, Wu, Shaoguang, Wu, Xinqun, and Sears, Cynthia
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BACTEROIDES fragilis ,DOUBLE-strand DNA breaks ,EPITHELIAL cells ,GENOMES ,GUT microbiome - Abstract
Background Individuals born in 1990 have twice the risk of developing colon cancer and four times the risk of developing rectal cancer as those born in 1950. The gut microbiome is being proposed as a potential contributor to this difference because of the surge in obesity in the United States, the link between obesity and gut dysbiosis, and the growing number of studies which have associated a dysbiotic gut microbiome with CRC. Enterotoxigenic Bacteroides fragilis (ETBF) is one of the bacteria most studied in relation to CRC development; it is found at a higher frequency in both the stool and mucosa of CRC patients, and it rapidly induces tumor formation in an Apc
min/+ mouse model of CRC. In this model, tumor formation typically occurs via loss of heterozygosity (LOH) of the Apc gene, the genetic mutation found in approximately 80% of sporadic CRC cases. ETBF produces a potent exotoxin (BFT) which induces E-cadherin cleavage, β-catenin nuclear localization and colonic epithelial cell proliferation. But we still do not understand how these downstream effects cause lasting changes in the genome of colon epithelial cells that then initiate tumor formation and growth. As cancer is ultimately a disease that arises and progresses via changes in the genome, understanding these interactions is essential. Methods We hypothesize that ETBF induces DNA mutations via BFT that encourage tumor formation, and enhance tumor growth. To test this hypothesis, we performed whole-exome sequencing on tumors and normal tissue isolated from Apcmin/+ mice after ETBF or sham inoculation. Additionally, we isolated colon organoids from Apcmin/+ mouse normal tissue (colonoids) and Apcmin/+ mouse tumors (tumoroids) after ETBF or sham inoculation. We performed in vitro DNA damage assays and qPCR for Apc LOH on these colon organoids. Results Our preliminary data indicate that ETBF-induced tumors have lower rates of Apc LOH and that double-stranded DNA breaks are observed as soon as 3-hours after BFT treatment of colonoids and as soon as 72-hours after ETBF inoculation. Conclusion These data suggest that in vivo, ETBF may induce mutations in cancer-driver genes which cause tumor formation via pathways other than somatic recombination at the Apc locus, a result we are now testing with additional (N = 19) whole-exome tumor sequencing in-progress. Disclosures All authors: No reported disclosures. [ABSTRACT FROM AUTHOR]- Published
- 2019
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