1. Determination of olprinone in human plasma utilizing liquid chromatography tandem mass spectrometry
- Author
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Zhu, Ping, Wen, Yu-Guan, Chen, Ji-Mei, Zhuang, Jian, Zhou, Zhi-Ling, Zheng, Shao-Yi, Wu, Ruo-Bin, Xiao, Xue-Jun, Lu, Cong, Fan, Rui-Xin, Guo, Hui-Ming, and Fan, Xiao-Ping
- Subjects
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CARDIOTONIC agents , *LIQUID chromatography , *TANDEM mass spectrometry , *QUANTITATIVE chemical analysis , *PHARMACOKINETICS , *BLOOD plasma , *INTRAVENOUS therapy - Abstract
Abstract: A sensitive and rapid method was developed for quantification of olprinone in human plasma utilizing liquid chromatography tandem mass spectrometry (LC–MS/MS). An aliquot of 1mL plasma sample was extracted with ethyl acetate–dichloromethane. Separation of olprinone and the milrinone (internal standard, IS) from the interferences was achieved on a C18 column followed by MS/MS detection. The analytes were monitored in the positive ionization mode. Multiple reaction monitoring using the transition of m/z 251→ m/z 155 and m/z 212→ m/z 140 was performed to quantify olprinone and IS, respectively. The method had a total chromatographic run time of 3min and linear calibration curves over the concentration range of 0.5–60ng/mL. The lower limit of quantification (LLOQ) was 0.5ng/mL. The intra- and inter-day precisions were less than 16.3% for low QC level, and 7.1% for other QC levels, respectively. The intra- and inter-day relative errors were ranged between −12.2% and 3.7% for three QC concentration levels. The validated method was successfully applied to the quantification of olprinone concentration in human plasma after intravenous (i.v.) administration of olprinone at a constant rate of infusion of 2μg/(kgmin) for 5min in order to evaluate the pharmacokinetics. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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