1. Novel Variants of Respiratory Syncytial Virus A ON1 Associated With Increased Clinical Severity of Bronchiolitis.
- Author
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Midulla, Fabio, Di Mattia, Greta, Nenna, Raffaella, Scagnolari, Carolina, Viscido, Agnese, Oliveto, Giuseppe, Petrarca, Laura, Frassanito, Antonella, Arima, Serena, Antonelli, Guido, and Pierangeli, Alessandra
- Subjects
BRONCHIOLITIS ,RESPIRATORY syncytial virus ,G proteins ,LOGISTIC regression analysis ,RESEARCH ,GENETICS ,BIOLOGICAL evolution ,RESEARCH methodology ,EVALUATION research ,MEDICAL cooperation ,SEVERITY of illness index ,COMPARATIVE studies ,BRONCHIOLE diseases ,GENOTYPES ,RESPIRATORY syncytial virus infections - Abstract
Background: A study of respiratory syncytial virus-A (RSV A) genotype ON1 genetic variability and clinical severity in infants hospitalized with bronchiolitis over 6 epidemic seasons (2012-2013 to 2017-2018) was carried out.Methods: From prospectively enrolled term infants hospitalized for bronchiolitis, samples positive for RSV A ON1 (N = 139) were sequenced in the second half of the G gene. Patients' clinical data were obtained from medical files and each infant was assigned a clinical severity score. ANOVA comparison and adjusted multinomial logistic regression were used to evaluate clinical severity score and clinical parameters.Results: The phylogenetic analysis of 54 strains showed 3 distinct clades; sequences in the last 2 seasons differed from previous seasons. The most divergent and numerous cluster of 2017-2018 strains was characterized by a novel pattern of amino acid changes, some in antigenic sites. Several amino acid changes altered predicted glycosylation sites, with acquisition of around 10 new O-glycosylation sites. Clinical severity of bronchiolitis increased in 2016-2017 and 2017-2018 and changed according to the epidemic seasons only.Conclusions: Amino acid changes in the hypervariable part of G protein may have altered functions and/or changed its immunogenicity, leading to an impact on disease severity. [ABSTRACT FROM AUTHOR]- Published
- 2020
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