1. Surface engineered bimetallic gold/silver nanoclusters for in situ imaging of mercury ions in living organisms.
- Author
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Wang, Jianping, Wang, Wang, Yang, Linlin, Zhao, Jun, Han, Guangmei, Yu, Xiaoping, ShenTu, Xuping, and Ye, Zihong
- Subjects
MERCURY ,METAL detectors ,HEAVY metals ,SILVER ,DETECTION limit ,ENVIRONMENTAL protection ,GOLD nanoparticles - Abstract
Chemical sensing for the sensitive and reliable detection of mercury(II) ions (Hg
2+ ) is of great importance in environmental protection, food safety, and biomedical applications. Due to the bio-enrichment property of Hg2+ in organisms, it is particularly meaningful to develop an effective tool that can in situ and rapidly monitor the level of Hg2+ in living organisms. In this work, we report ligand functionalized gold-silver bimetallic nanoclusters with bright red fluorescence as intracellular probes for imaging Hg2+ in living cells and zebrafish. The bimetallic nanoclusters of DTT-GSH@Au/AgNCs (DG-Au/AgNCs) with strong fluorescence that benefited from the synergistic effect of Au and Ag atoms were obtained through a one-pot synthesis method, incorporating glutathione (GSH) and dithiothreitol (DTT) as the reducers and functionalized ligands. Attractively, the bright red fluorescence of DG-Au/AgNCs could be rapidly and selectively quenched by Hg2+ within 1 min with a very low detection limit of 1.01 nM. Additionally, DG-Au/AgNCs had a great advantage in the detection of Hg2+ in living cells and zebrafish owing to its notably strong red fluorescence at 665 nm, which could avoid effectively auto-fluorescence interference from the organism. Such easily prepared bimetallic fluorescent nanoclusters would be expected to provide a noninvasive and sensitive approach in the detection of heavy metals in situ for environmental protection. [ABSTRACT FROM AUTHOR]- Published
- 2022
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