1. Development of a duplex SYBR Green I-based quantitative real-time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses
- Author
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Fusong Yu, Xiao Shifeng, Xiuqin Chen, Chen Shaoying, Cheng Xiaoxia, Shao Wang, Chen Shilong, and Lin Su
- Subjects
0301 basic medicine ,GPV ,viruses ,animal diseases ,Oropharynx ,law.invention ,Parvovirus ,chemistry.chemical_compound ,0302 clinical medicine ,Cloaca ,law ,Geese ,Transition Temperature ,Organic Chemicals ,Polymerase chain reaction ,Phylogeny ,biology ,virus diseases ,Viral Load ,Infectious Diseases ,Quantitative Real Time PCR ,Ducks ,Quinolines ,030211 gastroenterology & hepatology ,Muscovy duck parvovirus ,Short Report ,Duplex real-time PCR ,Genome, Viral ,Diamines ,Real-Time Polymerase Chain Reaction ,lcsh:Infectious and parasitic diseases ,MDPV ,Parvoviridae Infections ,03 medical and health sciences ,Goose ,Virology ,biology.animal ,Animals ,lcsh:RC109-216 ,Benzothiazoles ,Poultry Diseases ,Goose parvovirus ,SYBR Green I ,biology.organism_classification ,030104 developmental biology ,chemistry ,Duplex (building) - Abstract
Background Waterfowl parvoviruses, including goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV), can cause seriously diseases in geese and ducks. Developing a fast and precise diagnosis assay for these two parvoviruses is particularly important. Results A duplex SYBR Green I-based quantitative real-time PCR assay was developed for the simultaneous detection and differentiation of GPV and MDPV. The assay yielded melting curves with specific single peak (Tm = 87.3 ± 0.26 °C or Tm = 85.4 ± 0.23 °C) when GPV or MDPV was evaluated, respectively. When both parvoviruses were assessed in one reaction, melting curves with specific double peaks were yielded. Conclusion This duplex quantitative RT-PCR can be used to rapid identify of GPV and MDPV in field cases and artificial trials, which make it a powerful tool for diagnosing, preventing and controlling waterfowl parvovirus infections.
- Published
- 2019