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After acute wound formation, the oxygen supply is reduced, which results in the formation of an acute hypoxic microenvironment; whether this hypoxic microenvironment enhances epidermal cell migration and the underlying regulatory molecular mechanism of this effect are unclear. In this study, HaCaT cells were maintained under hypoxic (1% oxygen) or normoxic conditions. Methods including immunofluorescence staining, wound scratch assays, transwell assays, Western blotting and high‐ and low‐expression lentiviral vector transfection were utilized to observe the changes in cell migration, microtubule dynamics and the expression levels of microtubule‐associated protein (MAP) 4 and the light chain protein DYNLT1 (Tctex‐1). The possible mechanisms were studied and discussed. The results showed that epidermal cell migration was enhanced during early hypoxia. Further experiments revealed that MAP4 regulates microtubule dynamics and promotes epidermal cell migration through Tctex‐1. MAP4 and Tctex‐1 play important roles in regulating the migration of epidermal cells under hypoxia. This evidence will provide a basis for further revealing the cellular and molecular mechanisms of local wound hypoxia and for promoting wound healing. [ABSTRACT FROM AUTHOR]

Nitroreductase (NTR) is an important biomarker widely used to evaluate the degree of tumor hypoxia. Although a few optical methods have been reported for detecting nitroreductase at low concentration ranges, an effective strategy for nitroreductase monitoring in vivo without limits to the imaging depth is still lacking. Herein, a novel dual‐mode NIR fluorescence and 19F MRI agent, FCy7‐NO2, is proposed for imaging tumor hypoxia. We show that FCy7‐NO2 serves as not only a rapid NIR fluorescence enhanced probe for monitoring and bioimaging of nitroreductase in tumors, but also a novel 19F MR chemical shift‐sensitive contrast agent for selectively detecting nitroreductase catalyzed reduction. Notably, integrating two complementary imaging technologies into FCy7‐NO2 enables sensitive detection of nitroreductase in a broad concentration range without tissue‐depth limit. In general, this agent has a remarkable response to nitroreductase, which provides a promising method for understanding tumor evolution and its physiological role in the hypoxic microenvironment. [ABSTRACT FROM AUTHOR]

Nitroreductase (NTR) is an important biomarker widely used to evaluate the degree of tumor hypoxia. Although a few optical methods have been reported for detecting nitroreductase at low concentration ranges, an effective strategy for nitroreductase monitoring in vivo without limits to the imaging depth is still lacking. Herein, a novel dual‐mode NIR fluorescence and 19F MRI agent, FCy7‐NO2, is proposed for imaging tumor hypoxia. We show that FCy7‐NO2 serves as not only a rapid NIR fluorescence enhanced probe for monitoring and bioimaging of nitroreductase in tumors, but also a novel 19F MR chemical shift‐sensitive contrast agent for selectively detecting nitroreductase catalyzed reduction. Notably, integrating two complementary imaging technologies into FCy7‐NO2 enables sensitive detection of nitroreductase in a broad concentration range without tissue‐depth limit. In general, this agent has a remarkable response to nitroreductase, which provides a promising method for understanding tumor evolution and its physiological role in the hypoxic microenvironment. [ABSTRACT FROM AUTHOR]

A bimodal imaging probe FCy7‐NO2 was applied to image nitroreductase overexpressed by lung cancer in vivo, as reported by Xin Zhou and co‐workers in their Research Article (e202213495). Integrating two complementary imaging technologies into FCy7‐NO2 enables sensitive detection of nitroreductase over a broad concentration range without tissue‐depth limit. [ABSTRACT FROM AUTHOR]

Inside Back Cover: In Vivo Nitroreductase Imaging via Fluorescence and Chemical Shift Dependent 19F NMR (Angew. Integrating two complementary imaging technologies into FCy7-NO SB 2 sb enables sensitive detection of nitroreductase over a broad concentration range without tissue-depth limit. 19F MRI, Biosensors, Fluorescence Imaging, Hypoxia, Nitroreductase. [Extracted from the article]

Eine bimodale Bildgebungssonde FCy7‐NO2 wurde eingesetzt, um die bei Lungenkrebs überexprimierte Nitroreduktase in vivo abzubilden, wie Xin Zhou et al. in ihrem Forschungsartikel berichten (e202213495). Die Integration zweier komplementärer Bildgebungstechnologien in FCy7‐NO2 ermöglicht einen empfindlichen Nachweis von Nitroreduktase in einem breiten Konzentrationsbereich ohne Gewebetiefenbegrenzung. [ABSTRACT FROM AUTHOR]

Keywords: 19F MRI; Biosensors; Fluorescence Imaging; Hypoxia; Nitroreductase EN 19F MRI Biosensors Fluorescence Imaging Hypoxia Nitroreductase 1 1 1 12/07/22 20221212 NES 221212 B Eine bimodale Bildgebungssonde b FCy7-NO SB 2 sb wurde eingesetzt, um die bei Lungenkrebs überexprimierte Nitroreduktase in vivo abzubilden, wie Xin Zhou et al. in ihrem Forschungsartikel berichten (e202213495). 19F MRI, Biosensors, Fluorescence Imaging, Hypoxia, Nitroreductase Die Integration zweier komplementärer Bildgebungstechnologien in FCy7-NO SB 2 sb ermöglicht einen empfindlichen Nachweis von Nitroreduktase in einem breiten Konzentrationsbereich ohne Gewebetiefenbegrenzung. [Extracted from the article]