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Delayed footpad reactions and acquired cellular resistance to Listeria monocytogenes were studied in mice whose mononuclear phagocyte system (MPS) had been blocked or stimulated. Colloidal carbon was used for the blockade of MPS and Corynebacterium parrum used for the stimulation. Strong delayed footpad reactions. On the other hand, the i.v. rejection of 3 × 101; listeria induced an appreciable level mice, while in MPS-stimulated mice, i.v. injection with even 4 × 103; listeria could not induce such strong delayed footpad reaction. On the other hand, the i.v. injection of 3 × 10¹ listeria induced an appreciable level of delayed footpad reaction only in MPS-blocked mice. Acquired cellular resistance was depressed by MPS stimulation, whereas it was augmented by MPS blockade. These results suggested that non-specific activity of MPS modulates subsequent immune responses after inoculation of listeria. [ABSTRACT FROM AUTHOR]

We have previously reported that heat-shock protein (hsp) 60-reactive T-cell receptor (TCR)γδ+ T cells appear in the peritoneal cavity during the early stage of infection with Listeria monocytogenes in mice. In this study, we examined the kinetics of TCRγδ+ T cells during listeriosis in F344 ruts by flow cytometry using a V65 monoclonal antibody (mAb) directed to a constant determinant of rat TCRγδ chains. TCRγδ+ T cells significantly increased in the peritoneal cavity on day 6 and then decreased by day 10 after infection, in parallel with the kinetics of hsp 60 expression in the peritoneal macrophages during listeriosis in F344 rats. Most of the early appearing TCRγδ+ T cells were of the CD4- CD8αβ+ CD5+ lymphocyte function-associated antigen (LFA)-1αhigh CD45RC- interleukin-2 receptor (IL-2R) α- phenotype, although a significant fraction of the TCRγδ+ T cells expressed CD8σ only. The increase in TCRγδ+ T cells during listeriosis was prominent in F1 (F344 × Lewis) rats but only marginal in Lewis rats, which was correlated with the expression level of hsp 60 in the peritoneal macrophages. The peritoneal TCRγδ+ T cells in naive F344 rats appeared to proliferate significantly in response to recombinant hsp 60 (rhsp 60) derived from Mycobacterium bovis bacillus Calmette-Guérin (BCG). These results imply that the early appearance of hsp 60-reactive TCRγδ+ T cells during listerial infection can be generalized across species. [ABSTRACT FROM AUTHOR]